Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001656

Physical Location & Seq

Genomic Coordinates Forward (+)

30543657 .. 30548777

Gene Structure 5121 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1225500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1383 bp

ATGGCGGCTGGTGGTGTTGGTGGTGGTCAGGTGGTGAAAGTTAAGAGGGAGAAGCTGGAGGCATGCATGACGTGCCCTCTTTGTAATAAGCTATTGAAGGAGGCTACAACCATTTCACTCTGCCTCCACACGTTTTGCAGGAAGTGCATCTTCGAAAAGCTCTCGGATGAGGAAGGGGACTGCTGTCCCGTCTGTGAAATTGATCTCGGAATTTTACCTGTCGAAAAACTCAGGCCAGACCATAATTTGCAAGATATTATAGCCAAGATATTTCCCGTTAAAGGAAAGAAAGTTGATACACCAGAAGCTAACGTGCCCGAAGCTAATGATCCTGATGTTACACCTTCTTCATTGCCAGTGAAAAGGAAGGAGAGATCATTATCTTCATTGGTAGTCAGTACCCCCAAAGTTCCATTACAGTCAGGTTTGACTGGAAAGAGATCAAGAGCTCTGTCAAGAAAGGGTGCTGTTTTACGAGGATGCAGTTTTTCCGCTGAAGAAGCTGCAAAGAGAGATGATTCTATAGAAGATCATCCAATGAGCTCCAACTCAACTGGTACTCTTGATGAATTCGCACAAAATAAGAGCCAGGATTCACCTGAGCTTTCATATGAGCGAACTGCTAATAAATATATGAACAATGACGTTGAAATAATAGAAGGGAAAGCTGATCTGTGGACGCCCTTAAACTGTCTCGTAGAGGCTGCTAACCGAACCAAGTCCTCGAAGCCAAATTCACAAGGGTTGCATGCCAAGTCTGATCCAACCAGTTCCCTTGATAGTGAACTTTATATGTCTCAAATTAAGGCAGAAGGAGAATTGCCAAATGCTCTGGACTGCATCAGGAATGCATATACGCCCAAAACCAAAAAAAAGGAGGCCGACCAGAAGGCAAGAGGAAAAAATAACAGTAACGGAACAGGGGCATCAGGACCAGTGAAACGTAAAAGGGTGCGCACAGCTAATCAGAACAGGGTAGCTGCATCTGGGGAATCAAGTGCCTCAACTCAACTTATGCTAGATACATGTGGGGCCAAGTATAGAAATGTTCCTGTTTGGTTCTCCTTGGTTGCATCTGAAGACTGGAAAGGAGAAATTTCATTGCCACAGATATCAGCATGTTGCTTGAAAATAAAGGATGGTAAAATGCCCGTATCAACAATCCAGAAGTACCTTGCAATGAAACTTGAACTTAGCAGTGAAAATGAAGTGGAGATACTGTGTCGGGGTCAGCCGGTGCTTCCAACATGGCAACTAGAATCCTTAGTAGAGTTGTGGTGCCGAACAGCACCAACAACAAAAAAAATACCGGTAACAGTTGGCTCCTCTGCTAAAGATTTTGTGATGGTCCTCAATTATTGTCGTAGGGTTCAAACTCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050896 GO:0051865 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_2

Protein Analysis

461

Amino Acids

50.29

Weight (kDa)

8.74

Isoelectric Point (pI)

51.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_2	PF13923	25 - 65	1.5e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	25 - 65	4.3e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001656)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06770 AT1G06770 AT1G06770 AT2G30580 AT2G30580 AT2G30580
fragaria_vesca	FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22360 FvH4_2g22400 FvH4_6g02710 FvH4_6g02710
malus_domestica	MD04G1225500.v1.1 MD05G1204700.v1.1 MD10G1191800.v1.1 MD12G1242500.v1.1
prunus_persica	Prupe.6G344100_v2.0.a1 Prupe.6G344100_v2.0.a1 Prupe.8G231300_v2.0.a1
pyrus_communis	pycom04g19880 pycom05g19090 pycom10g16540 pycom12g22160
rosa_chinensis	RchiOBHm_Chr3g0450721 RchiOBHm_Chr6g0289351
rosa_laevigata	RLG00000012310 RLG00000025707
rosa_multiflora	Rmu_sc0000078.1_g000029 Rmu_sc0041561.1_g000001
rosa_roxburghii	Rroxscaffold_6G00428390 Rroxscaffold_7G00179330
rosa_rugosa	Rorug02G0629900 Rorug02G0630000 Rorug06G0202900
rosa_samantha	Rh3AG030800 Rh3BG031300 Rh3CG030100 Rh3DG031000 Rh6AG313800 Rh6BG321300 Rh6CG327600 Rh6DG313200
rosa_wichuraiana	Rw3G002380 Rw6G027130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	956
AccB1I	GGYRCC	1 cut(s)	1278
AciI	CCGC	2 cut(s)	5, 492
AclWI	GGATC	2 cut(s)	323, 755
AcsI	RAATTY	4 cut(s)	210, 569, 733, 1095
AcuI	CTGAAG	2 cut(s)	516, 1098
AcyI	GRCGYC	1 cut(s)	680
AfaI	GTAC	3 cut(s)	400, 559, 1172
AfiI	CCNNNNNNNGG	1 cut(s)	281
AflIII	ACRYGT	2 cut(s)	129, 1025
AgeI	ACCGGT	1 cut(s)	1309
AgsI	TTSAA	5 cut(s)	97, 650, 1129, 1190, 1373
AjiI	CACGTC	1 cut(s)	72
AjnI	CCWGG	1 cut(s)	588
Alw21I	GWGCWC	2 cut(s)	451, 545
Alw26I	GTCTC	2 cut(s)	698, 801
AlwI	GGATC	2 cut(s)	323, 755
AoxI	GGCC	3 cut(s)	233, 879, 1032
ApeKI	GCWGC	3 cut(s)	503, 704, 980
ApoI	RAATTY	4 cut(s)	210, 569, 733, 1095
AsiGI	ACCGGT	1 cut(s)	1309
AspLEI	GCGC	1 cut(s)	957
AspS9I	GGNCC	3 cut(s)	932, 1032, 1348
AsuHPI	GGTGA	2 cut(s)	46, 588
AsuII	TTCGAA	1 cut(s)	153
AvaII	GGWCC	2 cut(s)	932, 1348
BaeGI	GKGCMC	2 cut(s)	77, 318
BanI	GGYRCC	1 cut(s)	1278
BanII	GRGCYC	2 cut(s)	451, 545
BarI	GAAGNNNNNNTAC	2 cut(s)	1200, 1232
BbsI	GAAGAC	1 cut(s)	1086
Bbv12I	GWGCWC	2 cut(s)	451, 545
BbvI	GCAGC	3 cut(s)	490, 691, 967
BccI	CCATC	2 cut(s)	1133, 1339
BciT130I	CCWGG	1 cut(s)	590
BcoDI	GTCTC	2 cut(s)	698, 801
BfaI	CTAG	2 cut(s)	1019, 1256
BfmI	CTRYAG	1 cut(s)	522
BisI	GCNGC	4 cut(s)	6, 504, 705, 981
BlsI	GCNGC	4 cut(s)	7, 505, 706, 982
Bme1390I	CCNGG	1 cut(s)	590
Bme18I	GGWCC	2 cut(s)	932, 1348
BmgBI	CACGTC	1 cut(s)	72
BmgT120I	GGNCC	3 cut(s)	932, 1032, 1348
BmiI	GGNNCC	3 cut(s)	1033, 1280, 1324
BmrFI	CCNGG	1 cut(s)	590
BmsI	GCATC	6 cut(s)	156, 470, 849, 935, 992, 1082
BoxI	GACNNNNGTC	1 cut(s)	183
BpiI	GAAGAC	1 cut(s)	1086
BpmI	CTGGAG	1 cut(s)	77
Bpu10I	CCTNAGC	1 cut(s)	600
Bpu14I	TTCGAA	1 cut(s)	153
BsaBI	GATNNNNATC	1 cut(s)	379
BsaHI	GRCGYC	1 cut(s)	680
BsaJI	CCNNGG	1 cut(s)	1065
BsaWI	WCCGGW	1 cut(s)	1309
BsaXI	ACNNNNNCTCC	4 cut(s)	108, 138, 1205, 1235
Bsc4I	CCNNNNNNNGG	1 cut(s)	281
Bse118I	RCCGGY	2 cut(s)	1234, 1309
Bse1I	ACTGG	6 cut(s)	356, 436, 559, 768, 935, 1088
Bse3DI	GCAATG	3 cut(s)	350, 1100, 1185
Bse8I	GATNNNNATC	1 cut(s)	379
BseBI	CCWGG	1 cut(s)	590
BseDI	CCNNGG	1 cut(s)	1065
BseGI	GGATG	4 cut(s)	172, 485, 532, 1144
BseJI	GATNNNNATC	1 cut(s)	379
BseLI	CCNNNNNNNGG	1 cut(s)	281
BseMI	GCAATG	3 cut(s)	350, 1100, 1185
BseMII	CTCAG	2 cut(s)	244, 591
BseNI	ACTGG	6 cut(s)	356, 436, 559, 768, 935, 1088
BseRI	GAGGAG	1 cut(s)	1315
BseSI	GKGCMC	2 cut(s)	77, 318
BseXI	GCAGC	3 cut(s)	490, 691, 967
BshFI	GGCC	3 cut(s)	235, 881, 1034
BshNI	GGYRCC	1 cut(s)	1278
BshTI	ACCGGT	1 cut(s)	1309
BsiHKAI	GWGCWC	2 cut(s)	451, 545
BsiSI	CCGG	2 cut(s)	1235, 1310
BslFI	GGGAC	2 cut(s)	171, 191
BslI	CCNNNNNNNGG	1 cut(s)	281
BsmAI	GTCTC	2 cut(s)	698, 801
BsmFI	GGGAC	2 cut(s)	171, 191
BsmI	GAATGC	1 cut(s)	853
BsnI	GGCC	3 cut(s)	235, 881, 1034
Bsp119I	TTCGAA	1 cut(s)	153
Bsp1286I	GDGCHC	4 cut(s)	77, 318, 451, 545
Bsp143I	GATC	7 cut(s)	202, 328, 374, 440, 529, 670, 760
BspACI	CCGC	2 cut(s)	5, 492
BspANI	GGCC	3 cut(s)	235, 881, 1034
BspCNI	CTCAG	2 cut(s)	243, 592
BspLI	GGNNCC	3 cut(s)	1033, 1280, 1324
BspPI	GGATC	2 cut(s)	323, 755
BspT104I	TTCGAA	1 cut(s)	153
BspT107I	GGYRCC	1 cut(s)	1278
BsrDI	GCAATG	3 cut(s)	350, 1100, 1185
BsrFI	RCCGGY	2 cut(s)	1234, 1309
BsrI	ACTGG	6 cut(s)	356, 436, 559, 768, 935, 1088
BssAI	RCCGGY	2 cut(s)	1234, 1309
BssECI	CCNNGG	1 cut(s)	1065
BssMI	GATC	7 cut(s)	202, 328, 374, 440, 529, 670, 760
BssNI	GRCGYC	1 cut(s)	680
BssT1I	CCWWGG	1 cut(s)	1065
Bst2UI	CCWGG	1 cut(s)	590
Bst4CI	ACNGT	5 cut(s)	420, 692, 911, 1221, 1318
BstACI	GRCGYC	1 cut(s)	680
BstAPI	GCANNNNNTGC	2 cut(s)	72, 144
BstBI	TTCGAA	1 cut(s)	153
BstC8I	GCNNGC	2 cut(s)	64, 750
BstDEI	CTNAG	4 cut(s)	230, 600, 1193, 1264
BstF5I	GGATG	4 cut(s)	172, 485, 532, 1144
BstHHI	GCGC	1 cut(s)	957
BstKTI	GATC	7 cut(s)	205, 331, 377, 443, 532, 673, 763
BstMAI	GTCTC	2 cut(s)	698, 801
BstMBI	GATC	7 cut(s)	202, 328, 374, 440, 529, 670, 760
BstMWI	GCNNNNNNNGC	3 cut(s)	72, 144, 500
BstNI	CCWGG	1 cut(s)	590
BstNSI	RCATGY	4 cut(s)	66, 752, 1029, 1122
BstPAI	GACNNNNGTC	1 cut(s)	183
BstSCI	CCNGG	1 cut(s)	588
BstSFI	CTRYAG	1 cut(s)	522
BstSLI	GKGCMC	2 cut(s)	77, 318
BstV1I	GCAGC	3 cut(s)	490, 691, 967
BstV2I	GAAGAC	1 cut(s)	1086
BsuRI	GGCC	3 cut(s)	235, 881, 1034
BtrI	CACGTC	1 cut(s)	72
BtsCI	GGATG	4 cut(s)	172, 485, 532, 1144
BtsI	GCAGTG	1 cut(s)	1204
BtsIMutI	CAGTG	3 cut(s)	363, 942, 1204
Cac8I	GCNNGC	2 cut(s)	64, 750
CfoI	GCGC	1 cut(s)	957
Cfr10I	RCCGGY	2 cut(s)	1234, 1309
Cfr13I	GGNCC	3 cut(s)	932, 1032, 1348
CseI	GACGC	1 cut(s)	688
Csp6I	GTAC	3 cut(s)	399, 558, 1171
CspAI	ACCGGT	1 cut(s)	1309
CspCI	CAANNNNNGTGG	2 cut(s)	116, 151
CviAII	CATG	7 cut(s)	63, 67, 749, 1026, 1119, 1248, 1380
CviQI	GTAC	3 cut(s)	399, 558, 1171
DdeI	CTNAG	4 cut(s)	230, 600, 1193, 1264
DpnI	GATC	7 cut(s)	204, 330, 376, 442, 531, 672, 762
DpnII	GATC	7 cut(s)	202, 328, 374, 440, 529, 670, 760
Ecl136II	GAGCTC	2 cut(s)	449, 543
Eco130I	CCWWGG	1 cut(s)	1065
Eco24I	GRGCYC	2 cut(s)	451, 545
Eco32I	GATATC	1 cut(s)	1113
Eco47I	GGWCC	2 cut(s)	932, 1348
Eco53kI	GAGCTC	2 cut(s)	449, 543
Eco57I	CTGAAG	2 cut(s)	516, 1098
EcoICRI	GAGCTC	2 cut(s)	449, 543
EcoRI	GAATTC	1 cut(s)	569
EcoRII	CCWGG	1 cut(s)	588
EcoRV	GATATC	1 cut(s)	1113
EcoT14I	CCWWGG	1 cut(s)	1065
EcoT22I	ATGCAT	2 cut(s)	68, 853
EcoT38I	GRGCYC	2 cut(s)	451, 545
ErhI	CCWWGG	1 cut(s)	1065
FaeI	CATG	7 cut(s)	66, 70, 752, 1029, 1122, 1251, 1383
FalI	AAGNNNNNCTT	2 cut(s)	134, 166
FaqI	GGGAC	2 cut(s)	171, 191
FatI	CATG	7 cut(s)	62, 66, 748, 1025, 1118, 1247, 1379
FauNDI	CATATG	1 cut(s)	610
Fnu4HI	GCNGC	4 cut(s)	6, 504, 705, 981
FokI	GGATG	4 cut(s)	179, 492, 519, 1151
FriOI	GRGCYC	2 cut(s)	451, 545
Fsp4HI	GCNGC	4 cut(s)	6, 504, 705, 981
FspAI	RTGCGCAY	1 cut(s)	956
FspBI	CTAG	2 cut(s)	1019, 1256
FspI	TGCGCA	1 cut(s)	956
GlaI	GCGC	1 cut(s)	956
GluI	GCNGC	4 cut(s)	6, 504, 705, 981
GsuI	CTGGAG	1 cut(s)	77
HaeIII	GGCC	3 cut(s)	235, 881, 1034
HapII	CCGG	2 cut(s)	1235, 1310
HgaI	GACGC	1 cut(s)	688
HhaI	GCGC	1 cut(s)	957
Hin1I	GRCGYC	1 cut(s)	680
Hin1II	CATG	7 cut(s)	66, 70, 752, 1029, 1122, 1251, 1383
Hin6I	GCGC	1 cut(s)	955
HinP1I	GCGC	1 cut(s)	955
HinfI	GANTC	4 cut(s)	518, 593, 992, 1259
HpaII	CCGG	2 cut(s)	1235, 1310
HphI	GGTGA	2 cut(s)	46, 588
Hpy166II	GTNNAC	2 cut(s)	678, 785
Hpy188I	TCNGA	5 cut(s)	166, 209, 760, 969, 1078
Hpy188III	TCNNGA	8 cut(s)	332, 444, 456, 563, 833, 844, 930, 1165
Hpy8I	GTNNAC	2 cut(s)	678, 785
HpyAV	CCTTC	7 cut(s)	91, 167, 354, 361, 653, 806, 883
HpyCH4III	ACNGT	5 cut(s)	420, 692, 911, 1221, 1318
HpyCH4IV	ACGT	5 cut(s)	71, 131, 312, 645, 943
HpyF10VI	GCNNNNNNNGC	3 cut(s)	72, 144, 500
HpyF3I	CTNAG	4 cut(s)	230, 600, 1193, 1264
HpySE526I	ACGT	5 cut(s)	71, 131, 312, 645, 943
Hsp92I	GRCGYC	1 cut(s)	680
Hsp92II	CATG	7 cut(s)	66, 70, 752, 1029, 1122, 1251, 1383
HspAI	GCGC	1 cut(s)	955
Kzo9I	GATC	7 cut(s)	202, 328, 374, 440, 529, 670, 760
LmnI	GCTCC	2 cut(s)	548, 1328
Lsp1109I	GCAGC	3 cut(s)	490, 691, 967
LweI	GCATC	6 cut(s)	156, 470, 849, 935, 992, 1082
MaeI	CTAG	2 cut(s)	1019, 1256
MaeII	ACGT	5 cut(s)	71, 131, 312, 645, 943
MaeIII	GTNAC	3 cut(s)	337, 911, 1312
MalI	GATC	7 cut(s)	204, 330, 376, 442, 531, 672, 762
MboI	GATC	7 cut(s)	202, 328, 374, 440, 529, 670, 760
MboII	GAAGA	6 cut(s)	142, 339, 375, 509, 539, 1091
MhlI	GDGCHC	4 cut(s)	77, 318, 451, 545
MluCI	AATT	9 cut(s)	198, 210, 244, 569, 733, 801, 818, 1095, 1354
MmeI	TCCRAC	3 cut(s)	570, 788, 1268
Mph1103I	ATGCAT	2 cut(s)	68, 853
MseI	TTAA	4 cut(s)	42, 279, 686, 804
MspA1I	CMGCKG	1 cut(s)	494
MspI	CCGG	2 cut(s)	1235, 1310
MspR9I	CCNGG	1 cut(s)	590
Mva1269I	GAATGC	1 cut(s)	853
MvaI	CCWGG	1 cut(s)	590
MwoI	GCNNNNNNNGC	3 cut(s)	72, 144, 500
NdeI	CATATG	1 cut(s)	610
NdeII	GATC	7 cut(s)	202, 328, 374, 440, 529, 670, 760
NlaIII	CATG	7 cut(s)	66, 70, 752, 1029, 1122, 1251, 1383
NlaIV	GGNNCC	3 cut(s)	1033, 1280, 1324
NsbI	TGCGCA	1 cut(s)	956
NsiI	ATGCAT	2 cut(s)	68, 853
NspI	RCATGY	4 cut(s)	66, 752, 1029, 1122
NspV	TTCGAA	1 cut(s)	153
PaeI	GCATGC	2 cut(s)	66, 752
PciI	ACATGT	1 cut(s)	1025
PctI	GAATGC	1 cut(s)	853
PfeI	GAWTC	4 cut(s)	518, 593, 992, 1259
PinAI	ACCGGT	1 cut(s)	1309
PkrI	GCNGC	4 cut(s)	7, 505, 706, 982
PscI	ACATGT	1 cut(s)	1025
PshAI	GACNNNNGTC	1 cut(s)	183
Psp124BI	GAGCTC	2 cut(s)	451, 545
Psp6I	CCWGG	1 cut(s)	588
PspGI	CCWGG	1 cut(s)	588
PspN4I	GGNNCC	3 cut(s)	1033, 1280, 1324
PspPI	GGNCC	3 cut(s)	932, 1032, 1348
RsaI	GTAC	3 cut(s)	400, 559, 1172
RsaNI	GTAC	3 cut(s)	399, 558, 1171
SacI	GAGCTC	2 cut(s)	451, 545
SaqAI	TTAA	4 cut(s)	42, 279, 686, 804
SatI	GCNGC	4 cut(s)	6, 504, 705, 981
Sau3AI	GATC	7 cut(s)	202, 328, 374, 440, 529, 670, 760
Sau96I	GGNCC	3 cut(s)	932, 1032, 1348
ScrFI	CCNGG	1 cut(s)	590
SduI	GDGCHC	4 cut(s)	77, 318, 451, 545
SfaNI	GCATC	6 cut(s)	156, 470, 849, 935, 992, 1082
SfcI	CTRYAG	1 cut(s)	522
SfuI	TTCGAA	1 cut(s)	153
SinI	GGWCC	2 cut(s)	932, 1348
SphI	GCATGC	2 cut(s)	66, 752
Sse9I	AATT	9 cut(s)	198, 210, 244, 569, 733, 801, 818, 1095, 1354
SsiI	CCGC	2 cut(s)	5, 492
SspMI	CTAG	2 cut(s)	1019, 1256
SstI	GAGCTC	2 cut(s)	451, 545
StyD4I	CCNGG	1 cut(s)	588
StyI	CCWWGG	1 cut(s)	1065
TaaI	ACNGT	5 cut(s)	420, 692, 911, 1221, 1318
TaiI	ACGT	5 cut(s)	74, 134, 315, 648, 946
TaqI	TCGA	3 cut(s)	153, 222, 725
TasI	AATT	9 cut(s)	198, 210, 244, 569, 733, 801, 818, 1095, 1354
TauI	GCSGC	1 cut(s)	8
TfiI	GAWTC	4 cut(s)	518, 593, 992, 1259
Tru1I	TTAA	4 cut(s)	42, 279, 686, 804
Tru9I	TTAA	4 cut(s)	42, 279, 686, 804
TscAI	CASTG	3 cut(s)	363, 942, 1204
TseI	GCWGC	3 cut(s)	503, 704, 980
TspDTI	ATGAA	8 cut(s)	339, 375, 582, 597, 650, 1089, 1196, 1221
TspGWI	ACGGA	1 cut(s)	930
TspRI	CASTG	3 cut(s)	363, 942, 1204
VpaK11BI	GGWCC	2 cut(s)	932, 1348
XapI	RAATTY	4 cut(s)	210, 569, 733, 1095
XceI	RCATGY	4 cut(s)	66, 752, 1029, 1122
XspI	CTAG	2 cut(s)	1019, 1256
Zsp2I	ATGCAT	2 cut(s)	68, 853

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.