MD04G1226600.v1.1

Domain in histone families 1 and 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003009
Physical Location & Seq
Forward (+)
30615370 .. 30616529
1160 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1226600.v1.1.491

Sequence Viewer

Length: 696 bp
ATGACTGGAACTGACGTCTCCGCCGCCAAGGCCAAGAAGGCGAGGTCTCCGCCCGCACACCCGCCGTTCGTCGAGATGATCACGATGGCGATTGTGGCGCTGAAGGAGAGGACGGGGTCGAGCCAGTACGCGATCACCAAGTTCGTGGAGGAGAAGCACAAGAATCTGCCGCCGACCTTCAGGAAGCTTCTGCTCTACCATTTGAAGAAGCTCGTGGCTGCCGGAAAGCTCGTCAAGGTGAAGAACTCGTTCAAGCTGCCTCCGGTTCGCGGCGCCGCTCCGGTGAAAGAGAAGACGGCCGCTGCACCGAAGCCTAAGAAGGCGGCGGTGGTCACCAAGCCGAAGACCAAAGCGGCTGCGAAGCCCAAGGCGAAGGTTGCGTCCAAGCCCAAGTCGAAAGCGGTTGCTGCTAAACCCAAGTCGAAAGCGGTTGCTGCTAAACCCAAGTCGAAAGCGGTTGCTGCTAAACCCAAGTCGGCAGCTGCGAAGCCAAAGGTGAAGAAGGCGGCGGCGGCGGCCAAGCCTGCTGCCAAGGCGGCAAGAACATCGACGAGGACGTCGCCGGGGAAGAAGGTGGACGTGAAGTCTAAGGCTAAGGCGAAGAAGCCGGTGGCTCCGGCGAGATCTGTGAGGAAGCCGAAGAGTGTGAAGTCTCCGGTGAAGAGGAAGGCTCCGGCGAGGAAGGCGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Linker_histone

Protein Analysis

232

Amino Acids

24.25

Weight (kDa)

11.43

Isoelectric Point (pI)

29.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 20 - 86 5.3e-18 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 556
AatII GACGTC 2 cut(s) 18, 560
AccB1I GGYRCC 1 cut(s) 272
AccBSI CCGCTC 1 cut(s) 278
AccII CGCG 2 cut(s) 131, 270
AcoI YGGCCR 2 cut(s) 297, 516
AcuI CTGAAG 2 cut(s) 122, 163
AcyI GRCGYC 3 cut(s) 15, 273, 557
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 1 cut(s) 269
AgsI TTSAA 2 cut(s) 205, 253
AhdI GACNNNNNGTC 1 cut(s) 583
AjiI CACGTC 1 cut(s) 580
AluBI AGCT 5 cut(s) 187, 211, 229, 256, 482
AluI AGCT 5 cut(s) 187, 211, 229, 256, 482
Alw26I GTCTC 3 cut(s) 22, 51, 657
AoxI GGCC 3 cut(s) 30, 297, 516
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
Asp700I GAANNNNTTC 1 cut(s) 248
AspLEI GCGC 2 cut(s) 100, 275
AsuC2I CCSGG 1 cut(s) 564
AsuHPI GGTGA 6 cut(s) 127, 250, 295, 325, 508, 670
BanI GGYRCC 1 cut(s) 272
BauI CACGAG 1 cut(s) 212
BbsI GAAGAC 2 cut(s) 299, 350
BccI CCATC 1 cut(s) 79
BceAI ACGGC 2 cut(s) 49, 312
BcgI CGANNNNNNTGC 2 cut(s) 528, 562
BclI TGATCA 1 cut(s) 78
BcnI CCSGG 1 cut(s) 564
BcoDI GTCTC 3 cut(s) 22, 51, 657
BfoI RGCGCY 2 cut(s) 101, 276
BglI GCCNNNNNGGC 3 cut(s) 29, 38, 536
BglII AGATCT 1 cut(s) 623
Bme1390I CCNGG 1 cut(s) 564
BmeRI GACNNNNNGTC 1 cut(s) 583
BmgBI CACGTC 1 cut(s) 580
BmiI GGNNCC 3 cut(s) 274, 615, 672
BmrFI CCNGG 1 cut(s) 564
BpiI GAAGAC 2 cut(s) 299, 350
BplI GAGNNNNNCTC 2 cut(s) 655, 687
Bpu10I CCTNAGC 1 cut(s) 594
BpuMI CCSGG 1 cut(s) 564
BsaHI GRCGYC 3 cut(s) 15, 273, 557
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 4 cut(s) 27, 366, 531, 563
BsaWI WCCGGW 3 cut(s) 262, 280, 655
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 2 cut(s) 10, 124
BseDI CCNNGG 4 cut(s) 27, 366, 531, 563
BseLI CCNNNNNNNGG 1 cut(s) 269
BseNI ACTGG 2 cut(s) 10, 124
BseRI GAGGAG 1 cut(s) 164
BseX3I CGGCCG 1 cut(s) 297
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 2 cut(s) 131, 270
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 3 cut(s) 32, 299, 518
BshNI GGYRCC 1 cut(s) 272
BsiEI CGRYCG 1 cut(s) 300
BsiSI CCGG 8 cut(s) 222, 263, 281, 563, 608, 617, 656, 674
BslI CCNNNNNNNGG 1 cut(s) 269
BsmAI GTCTC 3 cut(s) 22, 51, 657
BsmBI CGTCTC 1 cut(s) 22
BsnI GGCC 3 cut(s) 32, 299, 518
Bso31I GGTCTC 1 cut(s) 51
Bsp143I GATC 3 cut(s) 78, 132, 623
BspANI GGCC 3 cut(s) 32, 299, 518
BspFNI CGCG 2 cut(s) 131, 270
BspLI GGNNCC 3 cut(s) 274, 615, 672
BspT107I GGYRCC 1 cut(s) 272
BspTNI GGTCTC 1 cut(s) 51
BsrBI CCGCTC 1 cut(s) 278
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 2 cut(s) 10, 124
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 4 cut(s) 27, 366, 531, 563
BssMI GATC 3 cut(s) 78, 132, 623
BssNI GRCGYC 3 cut(s) 15, 273, 557
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 3 cut(s) 27, 366, 531
Bst2BI CACGAG 1 cut(s) 212
Bst6I CTCTTC 2 cut(s) 635, 656
BstACI GRCGYC 3 cut(s) 15, 273, 557
BstC8I GCNNGC 2 cut(s) 54, 525
BstDEI CTNAG 3 cut(s) 315, 588, 594
BstEII GGTNACC 1 cut(s) 331
BstFNI CGCG 2 cut(s) 131, 270
BstH2I RGCGCY 2 cut(s) 101, 276
BstHHI GCGC 2 cut(s) 100, 275
BstKTI GATC 3 cut(s) 81, 135, 626
BstMAI GTCTC 3 cut(s) 22, 51, 657
BstMBI GATC 3 cut(s) 78, 132, 623
BstMCI CGRYCG 1 cut(s) 300
BstPI GGTNACC 1 cut(s) 331
BstSCI CCNGG 1 cut(s) 562
BstUI CGCG 2 cut(s) 131, 270
BstV2I GAAGAC 2 cut(s) 299, 350
BstX2I RGATCY 1 cut(s) 623
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 1 cut(s) 623
BstZI CGGCCG 1 cut(s) 297
BsuRI GGCC 3 cut(s) 32, 299, 518
BtrI CACGTC 1 cut(s) 580
Cac8I GCNNGC 2 cut(s) 54, 525
CfoI GCGC 2 cut(s) 100, 275
Cfr10I RCCGGY 1 cut(s) 607
CseI GACGC 1 cut(s) 369
Csp6I GTAC 1 cut(s) 127
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 3 cut(s) 315, 588, 594
DinI GGCGCC 1 cut(s) 274
DpnI GATC 3 cut(s) 80, 134, 625
DpnII GATC 3 cut(s) 78, 132, 623
DrdI GACNNNNNNGTC 1 cut(s) 556
DriI GACNNNNNGTC 1 cut(s) 583
DseDI GACNNNNNNGTC 1 cut(s) 556
EaeI YGGCCR 2 cut(s) 297, 516
EagI CGGCCG 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 635, 656
Eam1105I GACNNNNNGTC 1 cut(s) 583
EarI CTCTTC 2 cut(s) 635, 656
EciI GGCGGA 2 cut(s) 10, 39
EclXI CGGCCG 1 cut(s) 297
Eco130I CCWWGG 3 cut(s) 27, 366, 531
Eco31I GGTCTC 1 cut(s) 51
Eco52I CGGCCG 1 cut(s) 297
Eco57I CTGAAG 2 cut(s) 122, 163
Eco91I GGTNACC 1 cut(s) 331
EcoO65I GGTNACC 1 cut(s) 331
EcoT14I CCWWGG 3 cut(s) 27, 366, 531
EgeI GGCGCC 1 cut(s) 274
EheI GGCGCC 1 cut(s) 274
ErhI CCWWGG 3 cut(s) 27, 366, 531
Esp3I CGTCTC 1 cut(s) 22
FauI CCCGC 2 cut(s) 61, 69
FbaI TGATCA 1 cut(s) 78
GlaI GCGC 2 cut(s) 99, 274
HaeII RGCGCY 2 cut(s) 101, 276
HaeIII GGCC 3 cut(s) 32, 299, 518
HapII CCGG 8 cut(s) 222, 263, 281, 563, 608, 617, 656, 674
HgaI GACGC 1 cut(s) 369
HhaI GCGC 2 cut(s) 100, 275
Hin1I GRCGYC 3 cut(s) 15, 273, 557
Hin6I GCGC 2 cut(s) 98, 273
HinP1I GCGC 2 cut(s) 98, 273
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 1 cut(s) 163
HpaII CCGG 8 cut(s) 222, 263, 281, 563, 608, 617, 656, 674
HphI GGTGA 6 cut(s) 127, 250, 295, 325, 508, 670
Hpy166II GTNNAC 1 cut(s) 577
Hpy188III TCNNGA 3 cut(s) 73, 82, 181
Hpy8I GTNNAC 1 cut(s) 577
Hpy99I CGWCG 3 cut(s) 74, 553, 562
HpyAV CCTTC 9 cut(s) 31, 97, 187, 313, 367, 496, 565, 661, 676
HpyCH4IV ACGT 3 cut(s) 15, 557, 579
HpyCH4V TGCA 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 315, 588, 594
HpySE526I ACGT 3 cut(s) 15, 557, 579
Hsp92I GRCGYC 3 cut(s) 15, 273, 557
HspAI GCGC 2 cut(s) 98, 273
KasI GGCGCC 1 cut(s) 272
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 3 cut(s) 78, 132, 623
LmnI GCTCC 3 cut(s) 283, 619, 676
MaeII ACGT 3 cut(s) 15, 557, 579
MaeIII GTNAC 1 cut(s) 331
MalI GATC 3 cut(s) 80, 134, 625
MbiI CCGCTC 1 cut(s) 278
MboI GATC 3 cut(s) 78, 132, 623
MboII GAAGA 9 cut(s) 217, 253, 304, 355, 511, 580, 613, 652, 673
MflI RGATCY 1 cut(s) 623
Mly113I GGCGCC 1 cut(s) 273
MnlI CCTC 8 cut(s) 36, 102, 142, 270, 546, 624, 657, 672
MroXI GAANNNNTTC 1 cut(s) 248
MspA1I CMGCKG 2 cut(s) 302, 482
MspI CCGG 8 cut(s) 222, 263, 281, 563, 608, 617, 656, 674
MspR9I CCNGG 1 cut(s) 564
MvnI CGCG 2 cut(s) 131, 270
NarI GGCGCC 1 cut(s) 273
NciI CCSGG 1 cut(s) 564
NdeII GATC 3 cut(s) 78, 132, 623
NlaIV GGNNCC 3 cut(s) 274, 615, 672
NmuCI GTSAC 1 cut(s) 331
PcsI WCGNNNNNNNCGW 2 cut(s) 482, 554
PdmI GAANNNNTTC 1 cut(s) 248
PfeI GAWTC 1 cut(s) 163
PflFI GACNNNGTC 1 cut(s) 115
PluTI GGCGCC 1 cut(s) 276
PspEI GGTNACC 1 cut(s) 331
PspN4I GGNNCC 3 cut(s) 274, 615, 672
PsuI RGATCY 1 cut(s) 623
PsyI GACNNNGTC 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
Sau3AI GATC 3 cut(s) 78, 132, 623
ScrFI CCNGG 1 cut(s) 564
SfoI GGCGCC 1 cut(s) 274
SspDI GGCGCC 1 cut(s) 272
StyD4I CCNGG 1 cut(s) 562
StyI CCWWGG 3 cut(s) 27, 366, 531
TaiI ACGT 3 cut(s) 18, 560, 582
TaqI TCGA 6 cut(s) 72, 119, 395, 422, 449, 548
TfiI GAWTC 1 cut(s) 163
TseFI GTSAC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 331
Tth111I GACNNNGTC 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 248
ZraI GACGTC 2 cut(s) 16, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.