Rorug06G0202300

Histone H1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003009
Physical Location & Seq
Forward (+)
32458597 .. 32460185
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0202300.1

Sequence Viewer

Length: 786 bp
ATGGCAAGGAGTCAAGGACGTCCATTTTATTATGGAACTCATACAGAAGACAATGAGTACAAAGAAGAGGTAGAACCAACCTTGGAATTTGTGCTTACCAAGAAAGACATAACAGGGACTGGAGCACCAGCTACTCTGTTGGTTTTTAATAACGAGATTGAATTTTCCCAAAAGAACATTGATTCTATCTGCAGCGTGGGCCGTTCTACCAAGAAGGGGAAGAGGCAACAAGGCTTTATTGGAGAAAAAGGCATTGGATTTAAAAGTGTGTTTCTTGTGAGTTCACAGCCCCACATATTCAGCAATGGATATCGTGTCAGATTTAATGAAGAACCAGATCACAATTGTGGTATTGGGTATGTAGTGCCTGAATGCATGGATAAGGTGGAAGCTGTTCGAGAGCAACTGTCTAAGTTGCACCCAGAGCTTCTCCTCTTTTTAATAGTTTTATCCAAGATAAAGTGTCTAGATGTACGTGGTTGTGATCCTGAAGGAGCTCAATCAGTGTCTATAGTTTCTATATGTAGCCAGACTGATGATGAGGATCTTAGAGGCAAAACAGCCAAGTCTCATGTTGTTCAACTTTCAGTTAAAGAAAGCATGTGTGATAATCAGGATTTGTGCAAGTACTACTTGTGGAGAGAAGCATTTCCAGTTAAACCTGGGAATAGAGTTGGTATAAGGTCGGATGTGGAGAAATGGGTGGTTACCCTAGCTTTCCCATTTGGAGAAAGACTGAGACGGGGAACATCATGTGTTGGTGTCTTTGCCTTCTTGCCAACATGA

Protein Analysis

261

Amino Acids

29.31

Weight (kDa)

6.95

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 33 - 104 1.9e-09 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 22
AclWI GGATC 2 cut(s) 479, 552
AcsI RAATTY 2 cut(s) 86, 161
AcuI CTGAAG 1 cut(s) 510
AcyI GRCGYC 1 cut(s) 19
AfaI GTAC 3 cut(s) 59, 474, 629
AfiI CCNNNNNNNGG 1 cut(s) 216
AgsI TTSAA 2 cut(s) 161, 581
AjnI CCWGG 1 cut(s) 661
AjuI GAANNNNNNNTTGG 2 cut(s) 237, 269
AleI CACNNNNGTG 1 cut(s) 345
AluBI AGCT 5 cut(s) 131, 392, 427, 497, 716
AluI AGCT 5 cut(s) 131, 392, 427, 497, 716
Alw21I GWGCWC 2 cut(s) 127, 499
Alw26I GTCTC 2 cut(s) 573, 733
AlwI GGATC 2 cut(s) 479, 552
AlwNI CAGNNNCTG 1 cut(s) 119
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 1 cut(s) 192
ApoI RAATTY 2 cut(s) 86, 161
Asp700I GAANNNNTTC 2 cut(s) 393, 648
AspS9I GGNCC 1 cut(s) 199
BanII GRGCYC 1 cut(s) 499
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 2 cut(s) 127, 499
BbvI GCAGC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 1 cut(s) 663
BcoDI GTCTC 2 cut(s) 573, 733
BfaI CTAG 2 cut(s) 467, 713
BfmI CTRYAG 2 cut(s) 190, 510
BisI GCNGC 1 cut(s) 193
BlsI GCNGC 1 cut(s) 194
BmcAI AGTACT 1 cut(s) 629
Bme1390I CCNGG 1 cut(s) 663
BmgT120I GGNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 663
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 141
BsaAI YACGTR 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 19
BsaJI CCNNGG 2 cut(s) 81, 662
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse1I ACTGG 2 cut(s) 124, 653
Bse3DI GCAATG 1 cut(s) 310
Bse8I GATNNNNATC 1 cut(s) 543
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 2 cut(s) 81, 662
BseGI GGATG 1 cut(s) 694
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 728
BseNI ACTGG 2 cut(s) 124, 653
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 1 cut(s) 204
BshFI GGCC 1 cut(s) 201
BsiHKAI GWGCWC 2 cut(s) 127, 499
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 1 cut(s) 216
BsmAI GTCTC 2 cut(s) 573, 733
BsmBI CGTCTC 1 cut(s) 733
BsmFI GGGAC 1 cut(s) 130
BsmI GAATGC 1 cut(s) 377
BsnI GGCC 1 cut(s) 201
Bsp1286I GDGCHC 2 cut(s) 127, 499
Bsp143I GATC 3 cut(s) 337, 484, 544
BspANI GGCC 1 cut(s) 201
BspCNI CTCAG 1 cut(s) 729
BspMAI CTGCAG 1 cut(s) 194
BspPI GGATC 2 cut(s) 479, 552
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 2 cut(s) 124, 653
BssECI CCNNGG 2 cut(s) 81, 662
BssMI GATC 3 cut(s) 337, 484, 544
BssNI GRCGYC 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 663
Bst4CI ACNGT 1 cut(s) 408
Bst6I CTCTTC 2 cut(s) 60, 215
BstACI GRCGYC 1 cut(s) 19
BstBAI YACGTR 1 cut(s) 476
BstDEI CTNAG 3 cut(s) 411, 548, 737
BstEII GGTNACC 1 cut(s) 706
BstF5I GGATG 1 cut(s) 694
BstKTI GATC 3 cut(s) 340, 487, 547
BstMAI GTCTC 2 cut(s) 573, 733
BstMBI GATC 3 cut(s) 337, 484, 544
BstMWI GCNNNNNNNGC 2 cut(s) 198, 424
BstNI CCWGG 1 cut(s) 663
BstNSI RCATGY 1 cut(s) 604
BstPI GGTNACC 1 cut(s) 706
BstSCI CCNGG 1 cut(s) 661
BstSFI CTRYAG 2 cut(s) 190, 510
BstV1I GCAGC 1 cut(s) 204
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
BsuRI GGCC 1 cut(s) 201
BtsCI GGATG 1 cut(s) 694
BtsIMutI CAGTG 1 cut(s) 510
CaiI CAGNNNCTG 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 199
Csp6I GTAC 3 cut(s) 58, 473, 628
CviAII CATG 5 cut(s) 376, 572, 601, 753, 783
CviQI GTAC 3 cut(s) 58, 473, 628
DdeI CTNAG 3 cut(s) 411, 548, 737
DpnI GATC 3 cut(s) 339, 486, 546
DpnII GATC 3 cut(s) 337, 484, 544
DraI TTTAAA 1 cut(s) 262
Eam1104I CTCTTC 2 cut(s) 60, 215
EarI CTCTTC 2 cut(s) 60, 215
Ecl136II GAGCTC 1 cut(s) 497
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 499
Eco32I GATATC 1 cut(s) 311
Eco53kI GAGCTC 1 cut(s) 497
Eco57I CTGAAG 1 cut(s) 510
Eco91I GGTNACC 1 cut(s) 706
EcoICRI GAGCTC 1 cut(s) 497
EcoO65I GGTNACC 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 661
EcoRV GATATC 1 cut(s) 311
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 377
EcoT38I GRGCYC 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 733
FaeI CATG 5 cut(s) 379, 575, 604, 756, 786
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 1 cut(s) 130
FatI CATG 5 cut(s) 375, 571, 600, 752, 782
Fnu4HI GCNGC 1 cut(s) 193
FokI GGATG 1 cut(s) 701
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 1 cut(s) 193
FspBI CTAG 2 cut(s) 467, 713
GluI GCNGC 1 cut(s) 193
GsuI CTGGAG 1 cut(s) 141
HaeIII GGCC 1 cut(s) 201
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 5 cut(s) 379, 575, 604, 756, 786
HinfI GANTC 2 cut(s) 10, 182
Hpy166II GTNNAC 1 cut(s) 284
Hpy188I TCNGA 2 cut(s) 320, 688
Hpy188III TCNNGA 4 cut(s) 398, 467, 488, 614
Hpy8I GTNNAC 1 cut(s) 284
HpyAV CCTTC 3 cut(s) 208, 485, 781
HpyCH4III ACNGT 1 cut(s) 408
HpyCH4IV ACGT 2 cut(s) 19, 475
HpyCH4V TGCA 4 cut(s) 192, 375, 418, 624
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 424
HpyF3I CTNAG 3 cut(s) 411, 548, 737
HpySE526I ACGT 2 cut(s) 19, 475
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 5 cut(s) 379, 575, 604, 756, 786
Kzo9I GATC 3 cut(s) 337, 484, 544
LmnI GCTCC 2 cut(s) 122, 494
Lsp1109I GCAGC 1 cut(s) 204
MaeI CTAG 2 cut(s) 467, 713
MaeII ACGT 2 cut(s) 19, 475
MaeIII GTNAC 1 cut(s) 706
MalI GATC 3 cut(s) 339, 486, 546
MboI GATC 3 cut(s) 337, 484, 544
MboII GAAGA 4 cut(s) 59, 77, 232, 341
MfeI CAATTG 1 cut(s) 343
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 2 cut(s) 127, 499
MluCI AATT 3 cut(s) 86, 161, 343
MlyI GAGTC 1 cut(s) 19
MmeI TCCRAC 1 cut(s) 666
MnlI CCTC 5 cut(s) 61, 216, 443, 535, 545
Mph1103I ATGCAT 1 cut(s) 377
MroXI GAANNNNTTC 2 cut(s) 393, 648
MseI TTAA 6 cut(s) 147, 261, 324, 440, 591, 657
MslI CAYNNNNRTG 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 663
MunI CAATTG 1 cut(s) 343
Mva1269I GAATGC 1 cut(s) 377
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 2 cut(s) 198, 424
NdeII GATC 3 cut(s) 337, 484, 544
NlaIII CATG 5 cut(s) 379, 575, 604, 756, 786
NsiI ATGCAT 1 cut(s) 377
NspI RCATGY 1 cut(s) 604
OliI CACNNNNGTG 1 cut(s) 345
PctI GAATGC 1 cut(s) 377
PdmI GAANNNNTTC 2 cut(s) 393, 648
PfeI GAWTC 1 cut(s) 182
PkrI GCNGC 1 cut(s) 194
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
Ppu21I YACGTR 1 cut(s) 476
Psp124BI GAGCTC 1 cut(s) 499
Psp6I CCWGG 1 cut(s) 661
PspEI GGTNACC 1 cut(s) 706
PspGI CCWGG 1 cut(s) 661
PspPI GGNCC 1 cut(s) 199
PstI CTGCAG 1 cut(s) 194
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 544
RsaI GTAC 3 cut(s) 59, 474, 629
RsaNI GTAC 3 cut(s) 58, 473, 628
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 1 cut(s) 499
SaqAI TTAA 6 cut(s) 147, 261, 324, 440, 591, 657
SatI GCNGC 1 cut(s) 193
Sau3AI GATC 3 cut(s) 337, 484, 544
Sau96I GGNCC 1 cut(s) 199
ScaI AGTACT 1 cut(s) 629
SchI GAGTC 1 cut(s) 19
ScrFI CCNGG 1 cut(s) 663
SduI GDGCHC 2 cut(s) 127, 499
SfcI CTRYAG 2 cut(s) 190, 510
SmiMI CAYNNNNRTG 1 cut(s) 345
Sse9I AATT 3 cut(s) 86, 161, 343
SspMI CTAG 2 cut(s) 467, 713
SstI GAGCTC 1 cut(s) 499
StyD4I CCNGG 1 cut(s) 661
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 408
TaiI ACGT 2 cut(s) 22, 478
TaqI TCGA 1 cut(s) 397
TasI AATT 3 cut(s) 86, 161, 343
TatI WGTACW 2 cut(s) 57, 627
TfiI GAWTC 1 cut(s) 182
Tru1I TTAA 6 cut(s) 147, 261, 324, 440, 591, 657
Tru9I TTAA 6 cut(s) 147, 261, 324, 440, 591, 657
TscAI CASTG 1 cut(s) 510
TseI GCWGC 1 cut(s) 192
TspDTI ATGAA 1 cut(s) 342
TspRI CASTG 1 cut(s) 510
XapI RAATTY 2 cut(s) 86, 161
XbaI TCTAGA 1 cut(s) 466
XceI RCATGY 1 cut(s) 604
XmnI GAANNNNTTC 2 cut(s) 393, 648
XspI CTAG 2 cut(s) 467, 713
ZraI GACGTC 1 cut(s) 20
ZrmI AGTACT 1 cut(s) 629
Zsp2I ATGCAT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.