MD10G1191200.v1.1

Histone H1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003009
Physical Location & Seq
Reverse (-)
28799694 .. 28801231
1538 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1191200.v1.1.491

Sequence Viewer

Length: 891 bp
ATGGCGACCGAAGAAGCCACCGTGGTCGTCGTGACCGAGACGGTCGAGAATCCTCCTCCCGAGCCGGCCACCACCGAGACGGCGGCCGAAAAACCGCCGGCCAAGACCGGCAAGGCCAAGGAGCCCAAGGCGAAGAAGGCGCCTGCTCCGAGGAAGCCTAGAAGCGCTCCAGCTCACCCTCCGTACGAAGAGATGGTTAAGGACGCGATTGTGACGTTGAAGGAGAGGACTGGTTCGAGCCAGTACGCGATCACTAAGTTCATCGAGGATAAGCAAAAGCAGCTGCCGCCCAACTTCAAGAAGCTTCTGCTCTTCCATTTGAAGAAGCTTGTGAGTTCTAATAAGATCGTCAAGGTCAAAAACTCCTTCAAGCTCCCGCCTGTGAGGTCCTCGGTGCCGAAGCCTGCATCTCCGGTGAAGAAGAAGAAGCCGGCGGCCGCCGCTAAGCCGAAGGCAAAAGCTGTTGCTGCCAAGCCTAAGGGAGGGAAAAAACCAGCTGCCAAAGCTCCGGCAAAGCCAAAGGCTGCTGCCGCCAAGCCTAAGGCGAAACCGAAGGCAAAACCCGCGGCTAAGCCAAAAGCTGCTGCTCCGAAGCCCAAAGCGGCTGCTCCGGCAAAGCCTAAGCCTGCTGCCAAGCCTAAAGCCCCCGCCGCCAAGCCAAAAGCTGCCGCTGCCAAGCCCAAGGCGGCTGCTCCGAAGCCGAAGGCAAAGGAGAAGCCAGCGAAGGCGGCGAGGACACTGACGAGGTCATCACCCGGAAGGAAGGCTCCGACCGCCCGCCCTGCCCCGAAGAAAGCGGCGACGCCAAAGAAAGCTCCGGCCCGGAGCGTTAAGCCACGAAGCGTGAAGTCGCCGGCGAAGAAAGCTCCAGCTCGCAAGGGAAGAAAGTGA

Protein Analysis

297

Amino Acids

30.86

Weight (kDa)

10.96

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 59 - 125 2.1e-18 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 139, 394
AccII CGCG 3 cut(s) 206, 248, 566
AcoI YGGCCR 4 cut(s) 66, 84, 99, 435
AcyI GRCGYC 2 cut(s) 140, 803
AfaI GTAC 2 cut(s) 185, 245
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 482
AgsI TTSAA 4 cut(s) 220, 298, 322, 370
Alw26I GTCTC 2 cut(s) 32, 71
Ama87I CYCGRG 1 cut(s) 59
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 6 cut(s) 66, 84, 99, 114, 435, 819
AspLEI GCGC 2 cut(s) 142, 167
AspS9I GGNCC 2 cut(s) 387, 820
AsuC2I CCSGG 2 cut(s) 756, 823
AsuHPI GGTGA 3 cut(s) 167, 427, 744
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 1 cut(s) 387
AxyI CCTNAGG 2 cut(s) 477, 540
BanI GGYRCC 2 cut(s) 139, 394
BanII GRGCYC 1 cut(s) 126
BccI CCATC 1 cut(s) 187
BceAI ACGGC 1 cut(s) 96
BcnI CCSGG 2 cut(s) 756, 823
BcoDI GTCTC 2 cut(s) 32, 71
BfaI CTAG 1 cut(s) 159
BfoI RGCGCY 2 cut(s) 143, 168
BlpI GCTNAGC 2 cut(s) 444, 570
Bme1390I CCNGG 2 cut(s) 756, 823
Bme18I GGWCC 1 cut(s) 387
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 387, 820
BmiI GGNNCC 4 cut(s) 123, 141, 396, 768
BmrFI CCNGG 2 cut(s) 756, 823
BmsI GCATC 1 cut(s) 416
BpmI CTGGAG 2 cut(s) 153, 852
Bpu10I CCTNAGC 1 cut(s) 621
Bpu1102I GCTNAGC 2 cut(s) 444, 570
BpuMI CCSGG 2 cut(s) 756, 823
BsaHI GRCGYC 2 cut(s) 140, 803
BsaJI CCNNGG 7 cut(s) 21, 117, 126, 149, 390, 564, 681
BsaWI WCCGGW 1 cut(s) 412
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse118I RCCGGY 5 cut(s) 64, 97, 107, 430, 853
Bse1I ACTGG 2 cut(s) 235, 241
Bse21I CCTNAGG 2 cut(s) 477, 540
BseDI CCNNGG 7 cut(s) 21, 117, 126, 149, 390, 564, 681
BseLI CCNNNNNNNGG 1 cut(s) 482
BseNI ACTGG 2 cut(s) 235, 241
BseRI GAGGAG 1 cut(s) 45
BseX3I CGGCCG 2 cut(s) 84, 435
Bsh1236I CGCG 3 cut(s) 206, 248, 566
Bsh1285I CGRYCG 5 cut(s) 9, 45, 87, 438, 774
BshFI GGCC 6 cut(s) 68, 86, 101, 116, 437, 821
BshNI GGYRCC 2 cut(s) 139, 394
BsiEI CGRYCG 5 cut(s) 9, 45, 87, 438, 774
BsiHKCI CYCGRG 1 cut(s) 59
BsiWI CGTACG 1 cut(s) 183
BslI CCNNNNNNNGG 1 cut(s) 482
BsmAI GTCTC 2 cut(s) 32, 71
BsmBI CGTCTC 2 cut(s) 32, 71
BsnI GGCC 6 cut(s) 68, 86, 101, 116, 437, 821
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 2 cut(s) 249, 345
Bsp1720I GCTNAGC 2 cut(s) 444, 570
BspANI GGCC 6 cut(s) 68, 86, 101, 116, 437, 821
BspFNI CGCG 3 cut(s) 206, 248, 566
BspLI GGNNCC 4 cut(s) 123, 141, 396, 768
BspQI GCTCTTC 1 cut(s) 317
BspT107I GGYRCC 2 cut(s) 139, 394
BsrFI RCCGGY 5 cut(s) 64, 97, 107, 430, 853
BsrI ACTGG 2 cut(s) 235, 241
BssAI RCCGGY 5 cut(s) 64, 97, 107, 430, 853
BssECI CCNNGG 7 cut(s) 21, 117, 126, 149, 390, 564, 681
BssMI GATC 2 cut(s) 249, 345
BssNI GRCGYC 2 cut(s) 140, 803
BssT1I CCWWGG 3 cut(s) 117, 126, 681
Bst4CI ACNGT 2 cut(s) 22, 43
Bst6I CTCTTC 2 cut(s) 183, 317
BstACI GRCGYC 2 cut(s) 140, 803
BstDEI CTNAG 6 cut(s) 255, 444, 477, 540, 570, 621
BstDSI CCRYGG 2 cut(s) 21, 564
BstENI CCTNNNNNAGG 1 cut(s) 480
BstFNI CGCG 3 cut(s) 206, 248, 566
BstH2I RGCGCY 2 cut(s) 143, 168
BstHHI GCGC 2 cut(s) 142, 167
BstKTI GATC 2 cut(s) 252, 348
BstMAI GTCTC 2 cut(s) 32, 71
BstMBI GATC 2 cut(s) 249, 345
BstMCI CGRYCG 5 cut(s) 9, 45, 87, 438, 774
BstSCI CCNGG 2 cut(s) 754, 821
BstUI CGCG 3 cut(s) 206, 248, 566
BstZI CGGCCG 2 cut(s) 84, 435
Bsu36I CCTNAGG 2 cut(s) 477, 540
BsuRI GGCC 6 cut(s) 68, 86, 101, 116, 437, 821
BtgI CCRYGG 2 cut(s) 21, 564
BtsIMutI CAGTG 1 cut(s) 737
CciNI GCGGCCGC 1 cut(s) 435
CfoI GCGC 2 cut(s) 142, 167
Cfr10I RCCGGY 5 cut(s) 64, 97, 107, 430, 853
Cfr13I GGNCC 2 cut(s) 387, 820
Cfr42I CCGCGG 1 cut(s) 567
CseI GACGC 2 cut(s) 212, 811
Csp6I GTAC 2 cut(s) 184, 244
CviQI GTAC 2 cut(s) 184, 244
DdeI CTNAG 6 cut(s) 255, 444, 477, 540, 570, 621
DinI GGCGCC 1 cut(s) 141
DpnI GATC 2 cut(s) 251, 347
DpnII GATC 2 cut(s) 249, 345
EaeI YGGCCR 4 cut(s) 66, 84, 99, 435
EagI CGGCCG 2 cut(s) 84, 435
Eam1104I CTCTTC 2 cut(s) 183, 317
EarI CTCTTC 2 cut(s) 183, 317
EclXI CGGCCG 2 cut(s) 84, 435
Eco130I CCWWGG 3 cut(s) 117, 126, 681
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 387
Eco47III AGCGCT 1 cut(s) 166
Eco52I CGGCCG 2 cut(s) 84, 435
Eco81I CCTNAGG 2 cut(s) 477, 540
Eco88I CYCGRG 1 cut(s) 59
EcoNI CCTNNNNNAGG 1 cut(s) 480
EcoO109I RGGNCCY 1 cut(s) 387
EcoT14I CCWWGG 3 cut(s) 117, 126, 681
EcoT38I GRGCYC 1 cut(s) 126
EgeI GGCGCC 1 cut(s) 141
EheI GGCGCC 1 cut(s) 141
ErhI CCWWGG 3 cut(s) 117, 126, 681
Esp3I CGTCTC 2 cut(s) 32, 71
FauI CCCGC 4 cut(s) 384, 571, 655, 785
FriOI GRGCYC 1 cut(s) 126
FspBI CTAG 1 cut(s) 159
GlaI GCGC 2 cut(s) 141, 166
GsuI CTGGAG 2 cut(s) 153, 852
HaeII RGCGCY 2 cut(s) 143, 168
HaeIII GGCC 6 cut(s) 68, 86, 101, 116, 437, 821
HgaI GACGC 2 cut(s) 212, 811
HhaI GCGC 2 cut(s) 142, 167
Hin1I GRCGYC 2 cut(s) 140, 803
Hin6I GCGC 2 cut(s) 140, 165
HinP1I GCGC 2 cut(s) 140, 165
HindIII AAGCTT 2 cut(s) 302, 326
HinfI GANTC 1 cut(s) 49
HphI GGTGA 3 cut(s) 167, 427, 744
Hpy188I TCNGA 4 cut(s) 150, 591, 696, 771
Hpy188III TCNNGA 4 cut(s) 31, 46, 59, 298
Hpy99I CGWCG 2 cut(s) 32, 805
HpyAV CCTTC 9 cut(s) 130, 214, 376, 445, 547, 697, 718, 753, 757
HpyCH4III ACNGT 2 cut(s) 22, 43
HpyCH4IV ACGT 1 cut(s) 215
HpyCH4V TGCA 1 cut(s) 407
HpyF3I CTNAG 6 cut(s) 255, 444, 477, 540, 570, 621
HpySE526I ACGT 1 cut(s) 215
Hsp92I GRCGYC 2 cut(s) 140, 803
HspAI GCGC 2 cut(s) 140, 165
KasI GGCGCC 1 cut(s) 139
KroI GCCGGC 4 cut(s) 64, 97, 430, 853
KroNI GCCGGC 4 cut(s) 66, 99, 432, 855
KspI CCGCGG 1 cut(s) 567
Kzo9I GATC 2 cut(s) 249, 345
LguI GCTCTTC 1 cut(s) 317
LweI GCATC 1 cut(s) 416
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 2 cut(s) 31, 211
MalI GATC 2 cut(s) 251, 347
MboI GATC 2 cut(s) 249, 345
MhlI GDGCHC 1 cut(s) 126
Mly113I GGCGCC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 794
MreI CGCCGGCG 1 cut(s) 853
MroNI GCCGGC 4 cut(s) 64, 97, 430, 853
MseI TTAA 2 cut(s) 198, 831
MspA1I CMGCKG 4 cut(s) 283, 497, 566, 671
MspR9I CCNGG 2 cut(s) 756, 823
MvnI CGCG 3 cut(s) 206, 248, 566
NaeI GCCGGC 4 cut(s) 66, 99, 432, 855
NarI GGCGCC 1 cut(s) 140
NciI CCSGG 2 cut(s) 756, 823
NdeII GATC 2 cut(s) 249, 345
NgoMIV GCCGGC 4 cut(s) 64, 97, 430, 853
NlaIV GGNNCC 4 cut(s) 123, 141, 396, 768
NmuCI GTSAC 2 cut(s) 31, 211
NotI GCGGCCGC 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdiI GCCGGC 4 cut(s) 66, 99, 432, 855
PfeI GAWTC 1 cut(s) 49
Pfl23II CGTACG 1 cut(s) 183
PflFI GACNNNGTC 1 cut(s) 745
PluTI GGCGCC 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 387
PspLI CGTACG 1 cut(s) 183
PspN4I GGNNCC 4 cut(s) 123, 141, 396, 768
PspPI GGNCC 2 cut(s) 387, 820
PspPPI RGGWCCY 1 cut(s) 387
PsyI GACNNNGTC 1 cut(s) 745
PvuII CAGCTG 2 cut(s) 283, 497
RsaI GTAC 2 cut(s) 185, 245
RsaNI GTAC 2 cut(s) 184, 244
SacII CCGCGG 1 cut(s) 567
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 2 cut(s) 198, 831
Sau3AI GATC 2 cut(s) 249, 345
Sau96I GGNCC 2 cut(s) 387, 820
ScrFI CCNGG 2 cut(s) 756, 823
SduI GDGCHC 1 cut(s) 126
SfaNI GCATC 1 cut(s) 416
SfoI GGCGCC 1 cut(s) 141
Sfr303I CCGCGG 1 cut(s) 567
SgrAI CRCCGGYG 1 cut(s) 853
SgrBI CCGCGG 1 cut(s) 567
SinI GGWCC 1 cut(s) 387
SspDI GGCGCC 1 cut(s) 139
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 2 cut(s) 754, 821
StyI CCWWGG 3 cut(s) 117, 126, 681
TaaI ACNGT 2 cut(s) 22, 43
TaiI ACGT 1 cut(s) 218
TaqI TCGA 3 cut(s) 45, 236, 264
TaqII GACCGA 2 cut(s) 23, 50
TfiI GAWTC 1 cut(s) 49
Tru1I TTAA 2 cut(s) 198, 831
Tru9I TTAA 2 cut(s) 198, 831
TscAI CASTG 1 cut(s) 744
TseFI GTSAC 2 cut(s) 31, 211
Tsp45I GTSAC 2 cut(s) 31, 211
TspDTI ATGAA 1 cut(s) 250
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 1 cut(s) 744
Tth111I GACNNNGTC 1 cut(s) 745
VpaK11BI GGWCC 1 cut(s) 387
XagI CCTNNNNNAGG 1 cut(s) 480
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.