Rroxscaffold_7G00179420

Histone H1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003009
Physical Location & Seq
Forward (+)
19009115 .. 19020413
11299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179420.1

Sequence Viewer

Length: 762 bp
ATGGCCGAGGACGAACCGATCGTCGCCACCACCGAGACCGTCGAGCCCGCAACCGAGACCGTCGACCCCCCGCCCGCCGACGACAAGCCGGAGGCCAAGTCCGGCAAGGCCTCCAAGGCCAAGGAGCCCAAGGCCAAGAAGCCCGCTGCCCCCAAGAAGGCCAGGACTGCTCCCACTCATCCTCCTTATGAAGAGATGATCAAGGATGCGATTGTGACTCTTAAGGAGAGGACTGGTTCGAGCCAGTATGCGATCACTAAGTTCATCGAGGAGAAGCACAAGCAGCTCCCTCCTAACTTCAAGAAGCTTCTGCTCTACCATTTGAAGAAGCTCGTCAGCTCCGATAAGCTCGCCAAGGTCAAAAGCTCCTATAAGATTCCGTCGGCTCGGTCCGCTGCAGCTCCACCTAAGCCCGCTGCTGCTCCGGCCAAGAAGAAGCCTGCCGCCGCGGCCAAGCCCAAGGCCAAGGCTGCTGCGAAGCCAAAGGGAAAGACCAAGTCGGTCAAGGCGGCGAGCAAGTCTCCGGCCAAGGCCAAGGCCGCTGCTGCTAAGCCAAAGCCGAAGCCCAAAGCCAAGCCTGCGGCAAAGGCAAAGCCTGCTCCGGCCAAGACCAAGGCTACTGCTCCGGCCAAGGCCAAGGCTGCTCCGGCCAAGGCAAAGGCAGCTCCGGCCAAGGCCAAGGCTGCCCGGCTGCCAAGCCTAAGGCAGCAGCTCCCAAGCCCAAACTTAAGGAGAAGCCAGCCAAGGCTGCGAGGACGTTGA

Protein Analysis

253

Amino Acids

26.57

Weight (kDa)

10.52

Isoelectric Point (pI)

52.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 60 - 121 8.8e-19 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 63
AccII CGCG 1 cut(s) 449
AcoI YGGCCR 8 cut(s) 3, 426, 450, 525, 603, 627, 648, 669
AfiI CCNNNNNNNGG 1 cut(s) 157
AflII CTTAAG 2 cut(s) 221, 727
AgsI TTSAA 2 cut(s) 301, 325
AjnI CCWGG 1 cut(s) 161
AluBI AGCT 9 cut(s) 286, 307, 331, 339, 349, 366, 401, 665, 712
AluI AGCT 9 cut(s) 286, 307, 331, 339, 349, 366, 401, 665, 712
Alw26I GTCTC 3 cut(s) 29, 50, 525
ArsI GACNNNNNNTTYG 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 390
AsuC2I CCSGG 1 cut(s) 688
AvaII GGWCC 1 cut(s) 390
AxyI CCTNAGG 1 cut(s) 701
BanII GRGCYC 2 cut(s) 48, 129
BciT130I CCWGG 1 cut(s) 163
BclI TGATCA 1 cut(s) 198
BcnI CCSGG 1 cut(s) 688
BcoDI GTCTC 3 cut(s) 29, 50, 525
BfmI CTRYAG 1 cut(s) 396
BfrI CTTAAG 2 cut(s) 221, 727
BglI GCCNNNNNGGC 2 cut(s) 116, 449
BlpI GCTNAGC 1 cut(s) 549
Bme1390I CCNGG 2 cut(s) 163, 688
Bme18I GGWCC 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 390
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 2 cut(s) 163, 688
BmsI GCATC 1 cut(s) 196
BplI GAGNNNNNCTC 2 cut(s) 505, 537
Bpu10I CCTNAGC 1 cut(s) 408
Bpu1102I GCTNAGC 1 cut(s) 549
BpuMI CCSGG 1 cut(s) 688
BsaI GGTCTC 2 cut(s) 29, 50
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 166, 196
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 2 cut(s) 238, 244
Bse21I CCTNAGG 1 cut(s) 701
BseBI CCWGG 1 cut(s) 163
BseGI GGATG 2 cut(s) 178, 211
BseLI CCNNNNNNNGG 1 cut(s) 157
BseNI ACTGG 2 cut(s) 238, 244
BseRI GAGGAG 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 449
Bsh1285I CGRYCG 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 21
BsiSI CCGG 9 cut(s) 89, 102, 425, 524, 602, 626, 647, 668, 688
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 3 cut(s) 29, 50, 525
Bso31I GGTCTC 2 cut(s) 29, 50
Bsp1286I GDGCHC 2 cut(s) 48, 129
Bsp143I GATC 3 cut(s) 18, 198, 252
Bsp1720I GCTNAGC 1 cut(s) 549
BspFNI CGCG 1 cut(s) 449
BspLI GGNNCC 1 cut(s) 126
BspMAI CTGCAG 1 cut(s) 400
BspTI CTTAAG 2 cut(s) 221, 727
BspTNI GGTCTC 2 cut(s) 29, 50
BsrI ACTGG 2 cut(s) 238, 244
BssMI GATC 3 cut(s) 18, 198, 252
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 2 cut(s) 40, 61
Bst6I CTCTTC 1 cut(s) 186
BstAFI CTTAAG 2 cut(s) 221, 727
BstAPI GCANNNNNTGC 1 cut(s) 596
BstDEI CTNAG 4 cut(s) 258, 408, 549, 701
BstDSI CCRYGG 1 cut(s) 447
BstF5I GGATG 2 cut(s) 178, 211
BstFNI CGCG 1 cut(s) 449
BstKTI GATC 3 cut(s) 21, 201, 255
BstMAI GTCTC 3 cut(s) 29, 50, 525
BstMBI GATC 3 cut(s) 18, 198, 252
BstMCI CGRYCG 1 cut(s) 21
BstNI CCWGG 1 cut(s) 163
BstSCI CCNGG 2 cut(s) 161, 686
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 1 cut(s) 449
Bsu36I CCTNAGG 1 cut(s) 701
BtgI CCRYGG 1 cut(s) 447
BtsCI GGATG 2 cut(s) 178, 211
Cfr13I GGNCC 1 cut(s) 390
Cfr42I CCGCGG 1 cut(s) 450
CpoI CGGWCCG 1 cut(s) 390
CspI CGGWCCG 1 cut(s) 390
DdeI CTNAG 4 cut(s) 258, 408, 549, 701
DpnI GATC 3 cut(s) 20, 200, 254
DpnII GATC 3 cut(s) 18, 198, 252
EaeI YGGCCR 8 cut(s) 3, 426, 450, 525, 603, 627, 648, 669
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Eco147I AGGCCT 1 cut(s) 110
Eco24I GRGCYC 2 cut(s) 48, 129
Eco31I GGTCTC 2 cut(s) 29, 50
Eco47I GGWCC 1 cut(s) 390
Eco81I CCTNAGG 1 cut(s) 701
EcoRII CCWGG 1 cut(s) 161
EcoT38I GRGCYC 2 cut(s) 48, 129
FaiI YATR 3 cut(s) 189, 249, 372
FauI CCCGC 5 cut(s) 55, 78, 82, 151, 421
FbaI TGATCA 1 cut(s) 198
FblI GTMKAC 1 cut(s) 63
FokI GGATG 2 cut(s) 165, 218
FriOI GRGCYC 2 cut(s) 48, 129
HapII CCGG 9 cut(s) 89, 102, 425, 524, 602, 626, 647, 668, 688
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 2 cut(s) 217, 376
HpaII CCGG 9 cut(s) 89, 102, 425, 524, 602, 626, 647, 668, 688
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 1 cut(s) 343
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 5 cut(s) 26, 44, 65, 83, 385
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 2 cut(s) 40, 61
HpyCH4IV ACGT 1 cut(s) 757
HpyCH4V TGCA 1 cut(s) 398
HpyF3I CTNAG 4 cut(s) 258, 408, 549, 701
HpySE526I ACGT 1 cut(s) 757
Ksp22I TGATCA 1 cut(s) 198
KspI CCGCGG 1 cut(s) 450
Kzo9I GATC 3 cut(s) 18, 198, 252
LweI GCATC 1 cut(s) 196
MaeII ACGT 1 cut(s) 757
MaeIII GTNAC 1 cut(s) 214
MalI GATC 3 cut(s) 20, 200, 254
MboI GATC 3 cut(s) 18, 198, 252
MboII GAAGA 3 cut(s) 203, 337, 445
MhlI GDGCHC 2 cut(s) 48, 129
MlyI GAGTC 1 cut(s) 211
MnlI CCTC 7 cut(s) 85, 121, 192, 222, 262, 300, 746
MseI TTAA 2 cut(s) 222, 728
MspA1I CMGCKG 5 cut(s) 146, 395, 416, 449, 542
MspCI CTTAAG 2 cut(s) 221, 727
MspI CCGG 9 cut(s) 89, 102, 425, 524, 602, 626, 647, 668, 688
MspR9I CCNGG 2 cut(s) 163, 688
MvaI CCWGG 1 cut(s) 163
MvnI CGCG 1 cut(s) 449
NciI CCSGG 1 cut(s) 688
NdeII GATC 3 cut(s) 18, 198, 252
NlaIV GGNNCC 1 cut(s) 126
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 214
PceI AGGCCT 1 cut(s) 110
PcsI WCGNNNNNNNCGW 3 cut(s) 18, 30, 339
PfeI GAWTC 1 cut(s) 376
PflFI GACNNNGTC 1 cut(s) 496
Ple19I CGATCG 1 cut(s) 21
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 1 cut(s) 390
PstI CTGCAG 1 cut(s) 400
PsyI GACNNNGTC 1 cut(s) 496
PvuI CGATCG 1 cut(s) 21
Rsr2I CGGWCCG 1 cut(s) 390
RsrII CGGWCCG 1 cut(s) 390
SacII CCGCGG 1 cut(s) 450
SalI GTCGAC 1 cut(s) 62
SaqAI TTAA 2 cut(s) 222, 728
Sau3AI GATC 3 cut(s) 18, 198, 252
Sau96I GGNCC 1 cut(s) 390
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 2 cut(s) 163, 688
SduI GDGCHC 2 cut(s) 48, 129
SfaNI GCATC 1 cut(s) 196
SfcI CTRYAG 1 cut(s) 396
SfiI GGCCNNNNNGGCC 1 cut(s) 116
Sfr303I CCGCGG 1 cut(s) 450
SgrBI CCGCGG 1 cut(s) 450
SinI GGWCC 1 cut(s) 390
SmlI CTYRAG 2 cut(s) 221, 727
SmoI CTYRAG 2 cut(s) 221, 727
SseBI AGGCCT 1 cut(s) 110
StuI AGGCCT 1 cut(s) 110
StyD4I CCNGG 2 cut(s) 161, 686
TaaI ACNGT 2 cut(s) 40, 61
TaiI ACGT 1 cut(s) 760
TaqI TCGA 4 cut(s) 42, 63, 239, 267
TaqII GACCGA 2 cut(s) 378, 490
TauI GCSGC 6 cut(s) 446, 449, 452, 512, 542, 584
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 2 cut(s) 222, 728
Tru9I TTAA 2 cut(s) 222, 728
TseFI GTSAC 1 cut(s) 214
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 204, 253
TspGWI ACGGA 1 cut(s) 369
Tth111I GACNNNGTC 1 cut(s) 496
Vha464I CTTAAG 2 cut(s) 221, 727
VpaK11BI GGWCC 1 cut(s) 390
XmiI GTMKAC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.