Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002016

Physical Location & Seq

Genomic Coordinates Forward (+)

31055451 .. 31059866

Gene Structure 4416 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1230700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2220 bp

ATGAAGGTGGTTGCTTTAGTAAGCGGCGGCAAGGACAGCTGCTATGCCATGATGAAGTGCATCCAATACGGCCACCAGATTGTTGCAGTGGCAAATTTGATGCCAGCTGATGATTCTGTGGACGAGCTTGATAGCTACATGTATCAAACTGTTGGGCACCAGATAGTCGTCAGCTATGCAGAATGCATGAGAGTCCCGTTGTTCAGAAGGAGAATACAAGGATCCACAAGGCATCAGAAGCTTAGCTATAGAATGACACCTGGCGATGAAGTCGAAGATATGTTTATTTTGTTAAATGAAGTGAAAAGACAGATTCCTTCTGTCACAGCAGTATCCTCTGGTGCTATTGCATCAGATTATCAAAGGCTGCGGGTGGAAAGTGTGTGTTCTAGGTTAGGGCTTGTTTCTCTAGCATACTTGTGGAAACAAGATCAGTCGATGCTCCTTCAAGAAATGATAACTAATGAGATTCTAGCAATTACAGTTAAGGTTGCTGCTATGGGGTTGGATCCCTCAAAGCACTTGGGCAAAGGAATAGCATTCTTACAACCCTATCTGCATAAGTTGAAAGATTTGTATGGTATTAATGTATGCGGCGAAGGAGGGGAATATGAAACCTTGACACTGGACTGCCCTCTCTTTGTTAATGCTAGAATTGTGCTGGATGAGTTTCAAATAATACTGCACTCTTCAGATTCCATAGCTCCGGTTGGGGTGCTTCATCCCTTGGCTTTTCATTTGGAAAATAAGGCAGAGTCCTGTACTTTGGGGAGCAGTGATCATACCAATCAGAAATGTCAGGAGAAGAAGAGTTTTCTATTTGAGGTGCAAGGAGACCATTCACAAGGATATGATGCTGCACGCAAAGCTGATGCCGAAGTTGAGAATGCAGTTGAACTTGCTGATTATAAACTTCACATTTCAAGAACACAAAAGGGTGATGCATTTTCTATTTGTTGCTGGTTACAAGATTCACGTACGACTTCTACAGGATTGCAAGGAGATCTGGAGGCTGTCCTGAAGAAAATTGAATCACTACTTGTAGAATATGGTTTTGGTTGGGAATATGTTCTCTATATTCATCTTTATATTGCCGACATGAAAGAGTTTGCTGCAGCAAATGATACATATGTGAGGTATATAACTCAAGGTAAGTGCCCTTTTGGTGTTCCATCCCGCAGTACAATTGAACTGCCTTTGTCGCAAGCGGGTTTAGGTAAGGCATACATGGAAGTTTTGGTGGCAAATGACCATTCCAAAAAGGTTCTACATGTACAGAGTATATCTTGCTGGGCACCCAGTTGTATTGGACCTTATAGCCAGGCAACTTTGCATAAGGAGATACTTCACATGGCTGGGCAGTTGGGGCTTGATCCTCCCACAATGACATTATGCCAAGGAGGCCCCATTGACGAGCTGGAAAAGGCACTAGAAAATAGTGAAGCAGTGGCTAAATCCTTTAACTGTTCAATATCAACCTCAGCTATTGTTTTTGTCACTTACTGTTCAACAAAAATTCTATCGTCAGAGAGATTTAAAATTCAGGAGAAGCAGGTTGCCTTCCTTAAACAAACAAGGAGCTTCGAGTTGGATCTAGGAACCAATTCTGAAGCATTTGATCCCATTCTTCTATATGTGCTTGTCCCTGATCTACCAAAAGGGGCCCTTGTAGAAATTAAGCCCATTTTATTTGTTGCAGAAGATATCGAAGAACCAGCTGAGAATGCAAAAGACCAATCCTGCTCAAGCACCCCTGGTTACTGGGGTTTTCAGCATGCAGACTGGCATGATTCTTGCTATCAGAGTTGCATTGTTCCTGGAAAATTATGTACGGTTGTTTTGTCCGTGAGTAGTGAACTTGCTTCAATGATCTGTCTGGAAGACAAGAACAAAGAGGATTATCAAAATTCTTTTGCAGAGAGGCAGATGGATAGAGTATCAAGATTTTGCATATATCTTCTGGATAAAATTATGACGGAGAACGCTTTTTCTTGGCAAGACACAATGTATTTGAGGTTGTATTGTCCAACAAGCCTTCATGTATCTGTGGATGCACTCTCACTGATGTTGACTAATGCATTCAATGAACTCGCCGAAATGGGTCGGATCAAGACTGGCAAGGAACCCGTCTTCAACCTCATTCCGGTCTTAGGTGCTGGGAGTTCTTCAGCAACCATGAATGATATAATCACCTGTGAATTATTGGCCCAGAAGTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Diphthami_syn_2 Pkinase Ribonuc_L-PSP

Protein Analysis

740

Amino Acids

82.09

Weight (kDa)

5.26

Isoelectric Point (pI)

43.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Diphthami_syn_2	PF01902	1 - 229	4e-36	Diphthamide synthase
Ribonuc_L-PSP	PF01042	329 - 398	2.6e-07	Endoribonuclease L-PSP
Ribonuc_L-PSP	PF01042	432 - 562	5.1e-09	Endoribonuclease L-PSP

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002016)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04480
fragaria_vesca	FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810
malus_domestica	MD04G1230700.v1.1
prunus_persica	Prupe.6G350500_v2.0.a1
pyrus_communis	pycom04g20440
rosa_chinensis	RchiOBHm_Chr2g0149091 RchiOBHm_Chr3g0449521 RchiOBHm_Chr6g0277751
rosa_laevigata	RLG00000025818 RLG00000036278
rosa_multiflora	Rmu_co8274617.1_g000001 Rmu_sc0000430.1_g000014
rosa_roxburghii	Rroxscaffold_6G00427480 Rroxscaffold_7G00175750
rosa_rugosa	Rorug02G0622400 Rorug02G0622500 Rorug02G0622600
rosa_samantha	Rh2AG410700 Rh2AG659700 Rh2BG670500 Rh2BG670600 Rh2CG396800 Rh3AG020100 Rh3BG020200 Rh3CG018900 Rh3DG020100
rosa_wichuraiana	Rw2G033330 Rw2G033560 Rw3G001600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	909
Acc36I	ACCTGC	1 cut(s)	1543
AccB1I	GGYRCC	2 cut(s)	156, 1294
AciI	CCGC	6 cut(s)	24, 27, 370, 594, 1177, 1208
AclWI	GGATC	8 cut(s)	216, 229, 503, 516, 1367, 1599, 1613, 2114
AcoI	YGGCCR	1 cut(s)	70
AcsI	RAATTY	4 cut(s)	94, 1515, 1539, 1906
AcuI	CTGAAG	4 cut(s)	675, 1040, 1629, 2151
AfaI	GTAC	5 cut(s)	763, 979, 1183, 1275, 1831
AfiI	CCNNNNNNNGG	2 cut(s)	2143, 2150
AflIII	ACRYGT	2 cut(s)	138, 1270
AjnI	CCWGG	4 cut(s)	259, 1320, 1753, 1816
AjuI	GAANNNNNNNTTGG	2 cut(s)	1038, 1070
Alw26I	GTCTC	1 cut(s)	828
AlwI	GGATC	8 cut(s)	216, 229, 503, 516, 1367, 1599, 1613, 2114
AoxI	GGCC	4 cut(s)	70, 1402, 1662, 2205
ApaI	GGGCCC	1 cut(s)	1666
ApeKI	GCWGC	6 cut(s)	39, 367, 494, 857, 1112, 1115
ApoI	RAATTY	4 cut(s)	94, 1515, 1539, 1906
AseI	ATTAAT	1 cut(s)	585
Asp700I	GAANNNNTTC	2 cut(s)	1068, 1603
AspS9I	GGNCC	5 cut(s)	1310, 1403, 1662, 1663, 2206
AsuHPI	GGTGA	2 cut(s)	950, 2182
AvaII	GGWCC	1 cut(s)	1310
BaeGI	GKGCMC	4 cut(s)	159, 1160, 1297, 1666
BaeI	ACNNNNGTAYC	4 cut(s)	315, 348, 1116, 1149
BamHI	GGATCC	2 cut(s)	221, 508
BanI	GGYRCC	2 cut(s)	156, 1294
BanII	GRGCYC	1 cut(s)	1666
BbsI	GAAGAC	2 cut(s)	1887, 2122
BbvCI	CCTCAGC	1 cut(s)	1480
BbvI	GCAGC	6 cut(s)	26, 354, 481, 844, 1099, 1127
BccI	CCATC	2 cut(s)	1180, 1921
BceAI	ACGGC	1 cut(s)	85
BciT130I	CCWGG	4 cut(s)	261, 1322, 1755, 1818
BciVI	GTATCC	1 cut(s)	343
BclI	TGATCA	1 cut(s)	778
BcoDI	GTCTC	1 cut(s)	828
BfaI	CTAG	6 cut(s)	390, 410, 473, 651, 1430, 1595
BfmI	CTRYAG	3 cut(s)	247, 987, 1113
BfuAI	ACCTGC	1 cut(s)	1543
BfuI	GTATCC	1 cut(s)	343
BglI	GCCNNNNNGGC	1 cut(s)	1401
BglII	AGATCT	1 cut(s)	1003
BisI	GCNGC	9 cut(s)	25, 28, 40, 368, 495, 595, 858, 1113, 1116
BlpI	GCTNAGC	1 cut(s)	242
BlsI	GCNGC	9 cut(s)	26, 29, 41, 369, 496, 596, 859, 1114, 1117
Bme1390I	CCNGG	4 cut(s)	261, 1322, 1755, 1818
Bme18I	GGWCC	1 cut(s)	1310
BmgT120I	GGNCC	5 cut(s)	1310, 1403, 1662, 1663, 2206
BmiI	GGNNCC	9 cut(s)	158, 223, 510, 1296, 1405, 1600, 1663, 1664, 2124
BmrFI	CCNGG	4 cut(s)	261, 1322, 1755, 1818
BmrI	ACTGGG	2 cut(s)	1293, 1771
BmsI	GCATC	9 cut(s)	69, 90, 241, 359, 429, 844, 862, 931, 2041
BmuI	ACTGGG	2 cut(s)	1293, 1771
BpiI	GAAGAC	2 cut(s)	1887, 2122
BpmI	CTGGAG	1 cut(s)	1028
Bpu10I	CCTNAGC	1 cut(s)	1480
Bpu1102I	GCTNAGC	1 cut(s)	242
BpuEI	CTTGAG	2 cut(s)	1131, 1729
BsaAI	YACGTR	1 cut(s)	977
BsaI	GGTCTC	1 cut(s)	828
BsaJI	CCNNGG	3 cut(s)	726, 1396, 1753
BsaWI	WCCGGW	2 cut(s)	706, 2143
Bsc4I	CCNNNNNNNGG	2 cut(s)	2143, 2150
Bse1I	ACTGG	5 cut(s)	630, 1299, 1766, 1787, 2119
BseBI	CCWGG	4 cut(s)	261, 1322, 1755, 1818
BseDI	CCNNGG	3 cut(s)	726, 1396, 1753
BseGI	GGATG	5 cut(s)	60, 670, 721, 1172, 2056
BseLI	CCNNNNNNNGG	2 cut(s)	2143, 2150
BseMII	CTCAG	2 cut(s)	1494, 1710
BseNI	ACTGG	5 cut(s)	630, 1299, 1766, 1787, 2119
BseSI	GKGCMC	4 cut(s)	159, 1160, 1297, 1666
BseXI	GCAGC	6 cut(s)	26, 354, 481, 844, 1099, 1127
BseYI	CCCAGC	3 cut(s)	1290, 1355, 2156
BsgI	GTGCAG	2 cut(s)	668, 843
BshFI	GGCC	4 cut(s)	72, 1404, 1664, 2207
BshNI	GGYRCC	2 cut(s)	156, 1294
BsiSI	CCGG	2 cut(s)	707, 2144
BsiWI	CGTACG	1 cut(s)	977
BslFI	GGGAC	2 cut(s)	179, 1628
BslI	CCNNNNNNNGG	2 cut(s)	2143, 2150
BsmAI	GTCTC	1 cut(s)	828
BsmFI	GGGAC	2 cut(s)	179, 1628
BsmI	GAATGC	5 cut(s)	188, 539, 892, 1729, 2078
BsnI	GGCC	4 cut(s)	72, 1404, 1664, 2207
Bso31I	GGTCTC	1 cut(s)	828
Bsp120I	GGGCCC	1 cut(s)	1662
Bsp1286I	GDGCHC	4 cut(s)	159, 1160, 1297, 1666
Bsp1407I	TGTACA	1 cut(s)	1273
Bsp1720I	GCTNAGC	1 cut(s)	242
BspACI	CCGC	6 cut(s)	24, 27, 370, 594, 1177, 1208
BspANI	GGCC	4 cut(s)	72, 1404, 1664, 2207
BspCNI	CTCAG	2 cut(s)	1493, 1711
BspLI	GGNNCC	9 cut(s)	158, 223, 510, 1296, 1405, 1600, 1663, 1664, 2124
BspMAI	CTGCAG	1 cut(s)	1117
BspMI	ACCTGC	1 cut(s)	1543
BspPI	GGATC	8 cut(s)	216, 229, 503, 516, 1367, 1599, 1613, 2114
BspT107I	GGYRCC	2 cut(s)	156, 1294
BspTNI	GGTCTC	1 cut(s)	828
BsrGI	TGTACA	1 cut(s)	1273
BsrI	ACTGG	5 cut(s)	630, 1299, 1766, 1787, 2119
BssECI	CCNNGG	3 cut(s)	726, 1396, 1753
BssT1I	CCWWGG	2 cut(s)	726, 1396
Bst2UI	CCWGG	4 cut(s)	261, 1322, 1755, 1818
Bst4CI	ACNGT	5 cut(s)	151, 484, 1466, 1505, 1834
Bst6I	CTCTTC	2 cut(s)	694, 803
BstAUI	TGTACA	1 cut(s)	1273
BstBAI	YACGTR	1 cut(s)	977
BstC8I	GCNNGC	4 cut(s)	105, 862, 1206, 1776
BstDEI	CTNAG	4 cut(s)	242, 1480, 1719, 2149
BstF5I	GGATG	5 cut(s)	60, 670, 721, 1172, 2056
BstMAI	GTCTC	1 cut(s)	828
BstMWI	GCNNNNNNNGC	7 cut(s)	36, 238, 866, 1201, 1366, 1401, 1724
BstNI	CCWGG	4 cut(s)	261, 1322, 1755, 1818
BstNSI	RCATGY	3 cut(s)	142, 1274, 1778
BstSCI	CCNGG	4 cut(s)	259, 1320, 1753, 1816
BstSFI	CTRYAG	3 cut(s)	247, 987, 1113
BstSLI	GKGCMC	4 cut(s)	159, 1160, 1297, 1666
BstV1I	GCAGC	6 cut(s)	26, 354, 481, 844, 1099, 1127
BstV2I	GAAGAC	2 cut(s)	1887, 2122
BstX2I	RGATCY	4 cut(s)	221, 508, 1003, 1591
BstYI	RGATCY	4 cut(s)	221, 508, 1003, 1591
BsuI	GTATCC	1 cut(s)	343
BsuRI	GGCC	4 cut(s)	72, 1404, 1664, 2207
BtgZI	GCGATG	1 cut(s)	279
BtsCI	GGATG	5 cut(s)	60, 670, 721, 1172, 2056
BtsI	GCAGTG	3 cut(s)	93, 781, 1452
BtsIMutI	CAGTG	5 cut(s)	93, 623, 781, 1452, 2060
BveI	ACCTGC	1 cut(s)	1543
Cac8I	GCNNGC	4 cut(s)	105, 862, 1206, 1776
Cfr13I	GGNCC	5 cut(s)	1310, 1403, 1662, 1663, 2206
Csp6I	GTAC	5 cut(s)	762, 978, 1182, 1274, 1830
CviQI	GTAC	5 cut(s)	762, 978, 1182, 1274, 1830
DdeI	CTNAG	4 cut(s)	242, 1480, 1719, 2149
DraI	TTTAAA	1 cut(s)	1537
EaeI	YGGCCR	1 cut(s)	70
Eam1104I	CTCTTC	2 cut(s)	694, 803
EarI	CTCTTC	2 cut(s)	694, 803
Eco130I	CCWWGG	2 cut(s)	726, 1396
Eco24I	GRGCYC	1 cut(s)	1666
Eco31I	GGTCTC	1 cut(s)	828
Eco32I	GATATC	1 cut(s)	1705
Eco47I	GGWCC	1 cut(s)	1310
Eco57I	CTGAAG	4 cut(s)	675, 1040, 1629, 2151
EcoO109I	RGGNCCY	3 cut(s)	1403, 1662, 1663
EcoRII	CCWGG	4 cut(s)	259, 1320, 1753, 1816
EcoRV	GATATC	1 cut(s)	1705
EcoT14I	CCWWGG	2 cut(s)	726, 1396
EcoT22I	ATGCAT	3 cut(s)	188, 946, 2080
EcoT38I	GRGCYC	1 cut(s)	1666
ErhI	CCWWGG	2 cut(s)	726, 1396
FalI	AAGNNNNNCTT	2 cut(s)	1650, 1682
FaqI	GGGAC	2 cut(s)	179, 1628
FauI	CCCGC	3 cut(s)	363, 1184, 1201
FauNDI	CATATG	1 cut(s)	1129
FbaI	TGATCA	1 cut(s)	778
Fnu4HI	GCNGC	9 cut(s)	25, 28, 40, 368, 495, 595, 858, 1113, 1116
FokI	GGATG	5 cut(s)	47, 677, 708, 1159, 2063
FriOI	GRGCYC	1 cut(s)	1666
Fsp4HI	GCNGC	9 cut(s)	25, 28, 40, 368, 495, 595, 858, 1113, 1116
FspBI	CTAG	6 cut(s)	390, 410, 473, 651, 1430, 1595
GluI	GCNGC	9 cut(s)	25, 28, 40, 368, 495, 595, 858, 1113, 1116
GsaI	CCCAGC	3 cut(s)	1294, 1359, 2160
GsuI	CTGGAG	1 cut(s)	1028
HaeIII	GGCC	4 cut(s)	72, 1404, 1664, 2207
HapII	CCGG	2 cut(s)	707, 2144
HincII	GTYRAC	1 cut(s)	2070
HindII	GTYRAC	1 cut(s)	2070
HindIII	AAGCTT	1 cut(s)	239
HinfI	GANTC	9 cut(s)	113, 192, 313, 469, 695, 755, 971, 1031, 1790
HpaII	CCGG	2 cut(s)	707, 2144
HphI	GGTGA	2 cut(s)	950, 2182
Hpy166II	GTNNAC	3 cut(s)	121, 1856, 2070
Hpy188I	TCNGA	9 cut(s)	206, 237, 355, 694, 792, 1528, 1609, 1803, 2106
Hpy8I	GTNNAC	3 cut(s)	121, 1856, 2070
HpyAV	CCTTC	6 cut(s)	201, 327, 455, 593, 1570, 2045
HpyCH4III	ACNGT	5 cut(s)	151, 484, 1466, 1505, 1834
HpyCH4IV	ACGT	1 cut(s)	976
HpyF10VI	GCNNNNNNNGC	7 cut(s)	36, 238, 866, 1201, 1366, 1401, 1724
HpyF3I	CTNAG	4 cut(s)	242, 1480, 1719, 2149
HpySE526I	ACGT	1 cut(s)	976
Ksp22I	TGATCA	1 cut(s)	778
LmnI	GCTCC	4 cut(s)	447, 709, 771, 1578
Lsp1109I	GCAGC	6 cut(s)	26, 354, 481, 844, 1099, 1127
LweI	GCATC	9 cut(s)	69, 90, 241, 359, 429, 844, 862, 931, 2041
MaeI	CTAG	6 cut(s)	390, 410, 473, 651, 1430, 1595
MaeII	ACGT	1 cut(s)	976
MaeIII	GTNAC	4 cut(s)	322, 963, 1495, 1757
MfeI	CAATTG	1 cut(s)	1185
MflI	RGATCY	4 cut(s)	221, 508, 1003, 1591
MhlI	GDGCHC	4 cut(s)	159, 1160, 1297, 1666
MlyI	GAGTC	2 cut(s)	201, 764
MmeI	TCCRAC	4 cut(s)	486, 1569, 2051, 2084
Mph1103I	ATGCAT	3 cut(s)	188, 946, 2080
MroXI	GAANNNNTTC	2 cut(s)	1068, 1603
MseI	TTAA	8 cut(s)	293, 486, 585, 645, 1461, 1536, 1566, 1677
MslI	CAYNNNNRTG	1 cut(s)	418
MspA1I	CMGCKG	3 cut(s)	39, 107, 1718
MspI	CCGG	2 cut(s)	707, 2144
MspR9I	CCNGG	4 cut(s)	261, 1322, 1755, 1818
MunI	CAATTG	1 cut(s)	1185
Mva1269I	GAATGC	5 cut(s)	188, 539, 892, 1729, 2078
MvaI	CCWGG	4 cut(s)	261, 1322, 1755, 1818
MwoI	GCNNNNNNNGC	7 cut(s)	36, 238, 866, 1201, 1366, 1401, 1724
NdeI	CATATG	1 cut(s)	1129
NlaIV	GGNNCC	9 cut(s)	158, 223, 510, 1296, 1405, 1600, 1663, 1664, 2124
NmuCI	GTSAC	2 cut(s)	322, 1495
NsiI	ATGCAT	3 cut(s)	188, 946, 2080
NspI	RCATGY	3 cut(s)	142, 1274, 1778
PaeI	GCATGC	1 cut(s)	1778
PciI	ACATGT	2 cut(s)	138, 1270
PctI	GAATGC	5 cut(s)	188, 539, 892, 1729, 2078
PdmI	GAANNNNTTC	2 cut(s)	1068, 1603
PfeI	GAWTC	7 cut(s)	113, 313, 469, 695, 971, 1031, 1790
Pfl23II	CGTACG	1 cut(s)	977
PfoI	TCCNGGA	1 cut(s)	1816
PkrI	GCNGC	9 cut(s)	26, 29, 41, 369, 496, 596, 859, 1114, 1117
PleI	GAGTC	2 cut(s)	200, 763
PpsI	GAGTC	2 cut(s)	200, 763
Ppu21I	YACGTR	1 cut(s)	977
PscI	ACATGT	2 cut(s)	138, 1270
PshBI	ATTAAT	1 cut(s)	585
PsiI	TTATAA	1 cut(s)	909
Psp6I	CCWGG	4 cut(s)	259, 1320, 1753, 1816
PspFI	CCCAGC	3 cut(s)	1290, 1355, 2156
PspGI	CCWGG	4 cut(s)	259, 1320, 1753, 1816
PspLI	CGTACG	1 cut(s)	977
PspN4I	GGNNCC	9 cut(s)	158, 223, 510, 1296, 1405, 1600, 1663, 1664, 2124
PspOMI	GGGCCC	1 cut(s)	1662
PspPI	GGNCC	5 cut(s)	1310, 1403, 1662, 1663, 2206
PstI	CTGCAG	1 cut(s)	1117
PsuI	RGATCY	4 cut(s)	221, 508, 1003, 1591
PvuII	CAGCTG	3 cut(s)	39, 107, 1718
RsaI	GTAC	5 cut(s)	763, 979, 1183, 1275, 1831
RsaNI	GTAC	5 cut(s)	762, 978, 1182, 1274, 1830
RseI	CAYNNNNRTG	1 cut(s)	418
SaqAI	TTAA	8 cut(s)	293, 486, 585, 645, 1461, 1536, 1566, 1677
SatI	GCNGC	9 cut(s)	25, 28, 40, 368, 495, 595, 858, 1113, 1116
Sau96I	GGNCC	5 cut(s)	1310, 1403, 1662, 1663, 2206
SchI	GAGTC	2 cut(s)	201, 764
ScrFI	CCNGG	4 cut(s)	261, 1322, 1755, 1818
SduI	GDGCHC	4 cut(s)	159, 1160, 1297, 1666
SfaNI	GCATC	9 cut(s)	69, 90, 241, 359, 429, 844, 862, 931, 2041
SfcI	CTRYAG	3 cut(s)	247, 987, 1113
SinI	GGWCC	1 cut(s)	1310
SmiMI	CAYNNNNRTG	1 cut(s)	418
SmlI	CTYRAG	2 cut(s)	1146, 1744
SmoI	CTYRAG	2 cut(s)	1146, 1744
SphI	GCATGC	1 cut(s)	1778
SsiI	CCGC	6 cut(s)	24, 27, 370, 594, 1177, 1208
SspMI	CTAG	6 cut(s)	390, 410, 473, 651, 1430, 1595
StyD4I	CCNGG	4 cut(s)	259, 1320, 1753, 1816
StyI	CCWWGG	2 cut(s)	726, 1396
TaaI	ACNGT	5 cut(s)	151, 484, 1466, 1505, 1834
TaiI	ACGT	1 cut(s)	979
TaqI	TCGA	4 cut(s)	273, 437, 1584, 1707
TatI	WGTACW	3 cut(s)	761, 1181, 1273
TauI	GCSGC	3 cut(s)	27, 30, 597
TfiI	GAWTC	7 cut(s)	113, 313, 469, 695, 971, 1031, 1790
Tru1I	TTAA	8 cut(s)	293, 486, 585, 645, 1461, 1536, 1566, 1677
Tru9I	TTAA	8 cut(s)	293, 486, 585, 645, 1461, 1536, 1566, 1677
TscAI	CASTG	5 cut(s)	93, 630, 781, 1452, 2067
TseFI	GTSAC	2 cut(s)	322, 1495
TseI	GCWGC	6 cut(s)	39, 367, 494, 857, 1112, 1115
Tsp45I	GTSAC	2 cut(s)	322, 1495
TspGWI	ACGGA	2 cut(s)	1834, 1991
TspRI	CASTG	5 cut(s)	93, 630, 781, 1452, 2067
VpaK11BI	GGWCC	1 cut(s)	1310
VspI	ATTAAT	1 cut(s)	585
XapI	RAATTY	4 cut(s)	94, 1515, 1539, 1906
XceI	RCATGY	3 cut(s)	142, 1274, 1778
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1414
XmnI	GAANNNNTTC	2 cut(s)	1068, 1603
XspI	CTAG	6 cut(s)	390, 410, 473, 651, 1430, 1595
Zsp2I	ATGCAT	3 cut(s)	188, 946, 2080

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.