Rh2CG396800

Diphthamide synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002016
Physical Location & Seq
Reverse (-)
53891395 .. 53895767
4373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG396800.1

Sequence Viewer

Length: 522 bp
ATGCTAGCTGATGATTCGGTGGACGAGCTTGATAGTTACATGTATCAAACTGTTGGGCACCAGATAGTTATCAGCTATGCAGAATGCATGGGAGTGCCATTGTTCAGAAGGCGAATACAAGGATCCACAAGGCATCAGAAGCTTAGCTATAGAATGACTCCTGGCGATGAAGTCGAAGATATGTTTATTTTGTTAAATGAAGTGAAACGACAGATTCCTTCTGTCACTGCTGTATCTTCTGGTGCTATTGCATCAGACTACCAAAGGTTGTGGGTGGAAAGTGTGTGTTCAAGGTTAGGTCTTGTTTCTCTAGCATACTTGTGGAAACAAGATCAGTCATTGCTCCTTCAAGAAATGATAACTAGTGGGATTGTGGCGAACAGTGAAGGCTCTATGATGCACGACTTCTTTATGAGAAATCATTGGCTTGTGTCGTCATATCAAGCTATCATCAGCCCCTTCAAGTACTTATGTAATCAATTGGAAGATAATTTTAATATAGCTAGCATGTTCTATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Diphthami_syn_2 Pkinase Ribonuc_L-PSP

Protein Analysis

173

Amino Acids

19.87

Weight (kDa)

5.1

Isoelectric Point (pI)

57.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthami_syn_2 PF01902 9 - 123 1.9e-14 Diphthamide synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 57
AclWI GGATC 2 cut(s) 117, 130
AfaI GTAC 1 cut(s) 467
AflIII ACRYGT 1 cut(s) 39
AgsI TTSAA 3 cut(s) 291, 350, 463
AhlI ACTAGT 1 cut(s) 362
AjnI CCWGG 1 cut(s) 160
AluBI AGCT 7 cut(s) 8, 28, 75, 142, 147, 446, 503
AluI AGCT 7 cut(s) 8, 28, 75, 142, 147, 446, 503
AlwI GGATC 2 cut(s) 117, 130
AsuNHI GCTAGC 2 cut(s) 4, 503
BaeGI GKGCMC 1 cut(s) 60
BaeI ACNNNNGTAYC 2 cut(s) 216, 249
BamHI GGATCC 1 cut(s) 122
BanI GGYRCC 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 162
BcuI ACTAGT 1 cut(s) 362
BfaI CTAG 4 cut(s) 5, 311, 363, 504
BfmI CTRYAG 1 cut(s) 148
BlpI GCTNAGC 1 cut(s) 143
BmcAI AGTACT 1 cut(s) 467
Bme1390I CCNGG 1 cut(s) 162
BmiI GGNNCC 2 cut(s) 59, 124
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 3 cut(s) 142, 260, 387
BmtI GCTAGC 2 cut(s) 8, 507
Bpu1102I GCTNAGC 1 cut(s) 143
BsaBI GATNNNNATC 1 cut(s) 68
Bse3DI GCAATG 1 cut(s) 338
Bse8I GATNNNNATC 1 cut(s) 68
BseBI CCWGG 1 cut(s) 162
BseJI GATNNNNATC 1 cut(s) 68
BseMI GCAATG 1 cut(s) 338
BseSI GKGCMC 1 cut(s) 60
BshNI GGYRCC 1 cut(s) 57
BsmI GAATGC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 2 cut(s) 122, 331
Bsp1720I GCTNAGC 1 cut(s) 143
BspLI GGNNCC 2 cut(s) 59, 124
BspOI GCTAGC 2 cut(s) 8, 507
BspPI GGATC 2 cut(s) 117, 130
BspT107I GGYRCC 1 cut(s) 57
BsrDI GCAATG 1 cut(s) 338
BssMI GATC 2 cut(s) 122, 331
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 52, 383
BstC8I GCNNGC 2 cut(s) 6, 505
BstDEI CTNAG 1 cut(s) 143
BstKTI GATC 2 cut(s) 125, 334
BstMBI GATC 2 cut(s) 122, 331
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstNI CCWGG 1 cut(s) 162
BstNSI RCATGY 2 cut(s) 43, 511
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 1 cut(s) 148
BstSLI GKGCMC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 122
BstYI RGATCY 1 cut(s) 122
BtgZI GCGATG 1 cut(s) 180
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 2 cut(s) 225, 388
Cac8I GCNNGC 2 cut(s) 6, 505
Csp6I GTAC 1 cut(s) 466
CspCI CAANNNNNGTGG 2 cut(s) 251, 286
CviAII CATG 3 cut(s) 40, 88, 508
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 1 cut(s) 143
DpnI GATC 2 cut(s) 124, 333
DpnII GATC 2 cut(s) 122, 331
EcoRII CCWGG 1 cut(s) 160
EcoT22I ATGCAT 1 cut(s) 89
FaeI CATG 3 cut(s) 43, 91, 511
FatI CATG 3 cut(s) 39, 87, 507
FspBI CTAG 4 cut(s) 5, 311, 363, 504
Hin1II CATG 3 cut(s) 43, 91, 511
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 3 cut(s) 14, 157, 214
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 3 cut(s) 107, 138, 256
Hpy188III TCNNGA 1 cut(s) 350
Hpy8I GTNNAC 1 cut(s) 22
HpyAV CCTTC 5 cut(s) 102, 228, 356, 380, 469
HpyCH4III ACNGT 2 cut(s) 52, 383
HpyCH4V TGCA 4 cut(s) 80, 87, 251, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 1 cut(s) 143
Hsp92II CATG 3 cut(s) 43, 91, 511
Kzo9I GATC 2 cut(s) 122, 331
LmnI GCTCC 1 cut(s) 348
LpnPI CCDG 4 cut(s) 74, 147, 174, 225
LweI GCATC 3 cut(s) 142, 260, 387
MaeI CTAG 4 cut(s) 5, 311, 363, 504
MaeIII GTNAC 2 cut(s) 35, 223
MalI GATC 2 cut(s) 124, 333
MboI GATC 2 cut(s) 122, 331
MboII GAAGA 3 cut(s) 188, 228, 497
MfeI CAATTG 1 cut(s) 479
MflI RGATCY 1 cut(s) 122
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 2 cut(s) 479, 490
MlyI GAGTC 1 cut(s) 151
Mph1103I ATGCAT 1 cut(s) 89
MseI TTAA 2 cut(s) 194, 495
MslI CAYNNNNRTG 2 cut(s) 92, 319
MspR9I CCNGG 1 cut(s) 162
MunI CAATTG 1 cut(s) 479
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 139
NdeII GATC 2 cut(s) 122, 331
NheI GCTAGC 2 cut(s) 4, 503
NlaIII CATG 3 cut(s) 43, 91, 511
NlaIV GGNNCC 2 cut(s) 59, 124
NmuCI GTSAC 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 2 cut(s) 43, 511
PciI ACATGT 1 cut(s) 39
PctI GAATGC 1 cut(s) 89
PfeI GAWTC 2 cut(s) 14, 214
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
PscI ACATGT 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 2 cut(s) 59, 124
PsuI RGATCY 1 cut(s) 122
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 2 cut(s) 92, 319
SaqAI TTAA 2 cut(s) 194, 495
Sau3AI GATC 2 cut(s) 122, 331
ScaI AGTACT 1 cut(s) 467
SchI GAGTC 1 cut(s) 151
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 1 cut(s) 60
SfaNI GCATC 3 cut(s) 142, 260, 387
SfcI CTRYAG 1 cut(s) 148
SmiMI CAYNNNNRTG 2 cut(s) 92, 319
SpeI ACTAGT 1 cut(s) 362
Sse9I AATT 2 cut(s) 479, 490
SspMI CTAG 4 cut(s) 5, 311, 363, 504
StyD4I CCNGG 1 cut(s) 160
TaaI ACNGT 2 cut(s) 52, 383
TaqI TCGA 1 cut(s) 174
TasI AATT 2 cut(s) 479, 490
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 2 cut(s) 14, 214
Tru1I TTAA 2 cut(s) 194, 495
Tru9I TTAA 2 cut(s) 194, 495
TscAI CASTG 2 cut(s) 232, 388
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 2 cut(s) 183, 213
TspRI CASTG 2 cut(s) 232, 388
XceI RCATGY 2 cut(s) 43, 511
XspI CTAG 4 cut(s) 5, 311, 363, 504
ZrmI AGTACT 1 cut(s) 467
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.