Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0002016

Physical Location & Seq

Genomic Coordinates Reverse (-)

48063065 .. 48069190

Gene Structure 6126 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00427480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGAAGGTGGTGGCTTTAGTAAGCGGCGGCAAGGACAGCTGCTATGCCATGATGAAGTGCCTCCAATACGGCCACCAGATTGTTGCTGTGGCAAATTTGATGCCAGCTGATGATTCGGTGGACGAGCTTGATAGTTACATGTATCAAACTGTAAGCCTCCTTCCAGTTGTCTATAATTCACCTTATTTGGATGTTTCTTCTTTCAAAAACAAAGACCAAATTTCTCAGAAGGCGAATACAAGGATCCACAAGGCAAGCCTTCTGAGAAGGGTATTTTGGCATCAGAAGCTTAGCTATAGAATGACTCCTGGCGATGAAGTCGAAGATATGTTTATTTTGTTAAATGAAGTGAAACGACAGATTCCTTCTGTCACTGCTGTATCTTCTGGTGCTATTGCATCAGACTACCAAAGGCTGCGGGTGGAAAGTGTCTGTTCAAGGTTAGGTCTTGTTTCTTTAGCATACTTGTGGAAACAAGATCAGTCTTTGCTCCTTCAAGAAATGATAACTAGTGGGATTGTGGCGATTACAGTGAAGGTTGCTGCCATGGGGTTGGACCCCTCAAAGCACTTGGGCAAAGAAATGTCAGTGTTGCAGCCGTATCTGCATAAGTTGAAAGAGTTATATGGTATTAATGTATGTGGTGAAGGAGGGGAATATGAAACGTTAACACTGGATTGCCCTCTCTTTGTTAATGCTCGAATCATGCTTGATGAGTCTCGAGTAGTAATGCACTCTTCTGATTCCATAGCTCCAGTTGGGGTGCTTCATCCGGTGGCTTTTCATTTGCAAAGTAAGGCAGAGTCTCATTCTTTGGAGAGCAATGACAGTGCTCATGAGATCTGTGAAGAGAAGGTGGGTTCTCTATTTGAAGTGCAAGGAGACACACAAGCATGTGATGCCATTTGCCAGGCTGATGCTGCAGTGGATGTCTTAGTTAAACTTCCTGAACATAAACTTCACATTTCAAGAACACAAAAGGACGATACATTTTCTATTTGTTGCTGGTTGCAAGATTCTTGTACAACTTCTGCAGGTTTGCAAGAAGACCTGGAGGCTGTTCTGAAGAAAATTGAATCGCTACTCGTGGAATATGGTTTTGGTTGGGAATATGTGCTCTACATTCATCTTTATATTGCTGATATGGGTGGGTTTTCTTCGGCAAACGATACATATGTGAGATATATAACCCAGGAGAAGTGCCCGTTTGGTGTTCCATCTCGCAGTACAGTTGAACTACCTTTGTTGCAAGTGGGTTTAGGTAATGCATACATGGAAGTTCTAGTAGCTAATGACCACACCAAAAGAGTTCTGCATGTACAAAGTATATCTTGTTGGGCACCCAGTTGTATTGGACCTTATAGCCAGGCAACTTTGCATAAGGAGATACTTCACATGGCTGGACAATTAGGGCTCGACCCTCCCACAATGACTTTATGTGATGGAGGTGTCACGGATGAGCTAGCAAAAGCACTGGACAATAGTGAAGCAGTGGCAAAAAGCTTTAACTGCTCTATATCTAATTCCACTATTATGTTTGTCAACTACTGTTCAACTAAAATTCCGCTGTCGGATAGATTAAATCTTCATGACAAGCATAAAGCTTTCCTTAAACAAACAAGGGACTCCAATTCAGATAAAGGAAGTAATTCTGAAGCATTGGAACCCATTTTCTTATATGTCCTTCTCCCGGATCTGCCAAAAGGGGCGCTTGTAGAAGTTAAGCCTGTTCTATTTGTTGCAGAAGATATTGAAGCAACAATTAGGGATGTGATAGACCAATCTCACTCAAGATCCACTGGTTACTGGGGTTTTCAACATGCAAATTGGCATGATTCCTGCATTCAAAAGTGCATTGTTCCTGAAAAGTTATGTGCAGTTGTTTTGTCTGTGAGTAGCGAACTCGCTGCCATGATCTGTGACAAGGACAAAGGGGTTCATCAAAATTCTGTCATAGGAAGGCAGATGGATAGAGTATTAAGATTTTGCTTATATCTTCTTGATAAGATTATCATGGAGAACGATTTTGGTTGGGAAGACACTATGTATTTGAGGTTCTACTTTCCTACTAGTCTTCAAGTGACTCTGGATGCACTGTCTTTCCTGTTGACCAATGCATTTAGTGAATTTGCTGAGATGAATCGGAATATCAAGATTGGCAAGGACCCCATCTTCAACCTAGTTCCTGTCTTAGGTGCTGGGAAGTCTTCCGCATCAATGAATGATATCATCACCTGTGAACTATTGGCTCGCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Diphthami_syn_2 Pkinase Ribonuc_L-PSP

Protein Analysis

752

Amino Acids

83.58

Weight (kDa)

5.42

Isoelectric Point (pI)

46.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Diphthami_syn_2	PF01902	105 - 246	2.4e-25	Diphthamide synthase
Ribonuc_L-PSP	PF01042	344 - 417	2.5e-07	Endoribonuclease L-PSP
Ribonuc_L-PSP	PF01042	447 - 513	9.6e-08	Endoribonuclease L-PSP

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002016)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04480
fragaria_vesca	FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810 FvH4_6g01810
malus_domestica	MD04G1230700.v1.1
prunus_persica	Prupe.6G350500_v2.0.a1
pyrus_communis	pycom04g20440
rosa_chinensis	RchiOBHm_Chr2g0149091 RchiOBHm_Chr3g0449521 RchiOBHm_Chr6g0277751
rosa_laevigata	RLG00000025818 RLG00000036278
rosa_multiflora	Rmu_co8274617.1_g000001 Rmu_sc0000430.1_g000014
rosa_roxburghii	Rroxscaffold_6G00427480 Rroxscaffold_7G00175750
rosa_rugosa	Rorug02G0622400 Rorug02G0622500 Rorug02G0622600
rosa_samantha	Rh2AG410700 Rh2AG659700 Rh2BG670500 Rh2BG670600 Rh2CG396800 Rh3AG020100 Rh3BG020200 Rh3CG018900 Rh3DG020100
rosa_wichuraiana	Rw2G033330 Rw2G033560 Rw3G001600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1025
AccB1I	GGYRCC	1 cut(s)	1339
AciI	CCGC	5 cut(s)	24, 27, 418, 1565, 2211
AclI	AACGTT	1 cut(s)	665
AclWI	GGATC	4 cut(s)	238, 251, 1701, 1788
AcoI	YGGCCR	1 cut(s)	70
AcsI	RAATTY	5 cut(s)	94, 219, 1560, 1945, 2126
AcuI	CTGAAG	2 cut(s)	1085, 1674
AfaI	GTAC	3 cut(s)	1024, 1228, 1320
AfiI	CCNNNNNNNGG	2 cut(s)	1690, 2192
AflIII	ACRYGT	1 cut(s)	138
AhlI	ACTAGT	2 cut(s)	509, 2069
AjnI	CCWGG	5 cut(s)	307, 909, 1050, 1191, 1365
AjuI	GAANNNNNNNTTGG	4 cut(s)	259, 291, 1083, 1115
Alw21I	GWGCWC	2 cut(s)	835, 1119
Alw26I	GTCTC	3 cut(s)	723, 810, 876
AlwI	GGATC	4 cut(s)	238, 251, 1701, 1788
Ama87I	CYCGRG	1 cut(s)	720
AoxI	GGCC	1 cut(s)	70
ApeKI	GCWGC	6 cut(s)	39, 415, 542, 595, 920, 1907
ApoI	RAATTY	5 cut(s)	94, 219, 1560, 1945, 2126
AseI	ATTAAT	1 cut(s)	633
Asp700I	GAANNNNTTC	1 cut(s)	1648
AspLEI	GCGC	1 cut(s)	1711
AspS9I	GGNCC	3 cut(s)	556, 1355, 2164
AsuC2I	CCSGG	1 cut(s)	1691
AsuHPI	GGTGA	3 cut(s)	171, 656, 2224
AsuNHI	GCTAGC	1 cut(s)	1462
AvaI	CYCGRG	1 cut(s)	720
AvaII	GGWCC	3 cut(s)	556, 1355, 2164
BaeGI	GKGCMC	2 cut(s)	1205, 1342
BaeI	ACNNNNGTAYC	4 cut(s)	363, 396, 1161, 1194
BamHI	GGATCC	1 cut(s)	243
BanI	GGYRCC	1 cut(s)	1339
BanII	GRGCYC	1 cut(s)	1416
BarI	GAAGNNNNNNTAC	2 cut(s)	144, 176
BauI	CACGAG	1 cut(s)	1085
BbsI	GAAGAC	4 cut(s)	1053, 2043, 2066, 2199
Bbv12I	GWGCWC	2 cut(s)	835, 1119
BbvI	GCAGC	6 cut(s)	26, 402, 529, 607, 907, 1894
BccI	CCATC	4 cut(s)	1225, 1436, 1960, 2177
BceAI	ACGGC	2 cut(s)	85, 583
BcgI	CGANNNNNNTGC	2 cut(s)	1889, 1923
BciT130I	CCWGG	5 cut(s)	309, 911, 1052, 1193, 1367
BcnI	CCSGG	1 cut(s)	1691
BcoDI	GTCTC	3 cut(s)	723, 810, 876
BcuI	ACTAGT	2 cut(s)	509, 2069
BfaI	CTAG	5 cut(s)	510, 1283, 1463, 2070, 2180
BfmI	CTRYAG	3 cut(s)	295, 921, 1032
BfoI	RGCGCY	1 cut(s)	1712
BfuAI	ACCTGC	1 cut(s)	1025
BglII	AGATCT	1 cut(s)	840
BisI	GCNGC	8 cut(s)	25, 28, 40, 416, 543, 596, 921, 1908
BlpI	GCTNAGC	1 cut(s)	290
BlsI	GCNGC	8 cut(s)	26, 29, 41, 417, 544, 597, 922, 1909
Bme1390I	CCNGG	6 cut(s)	309, 911, 1052, 1193, 1367, 1691
Bme18I	GGWCC	3 cut(s)	556, 1355, 2164
BmeT110I	CYCGRG	1 cut(s)	720
BmgT120I	GGNCC	3 cut(s)	556, 1355, 2164
BmiI	GGNNCC	5 cut(s)	245, 558, 1341, 1665, 2166
BmrFI	CCNGG	6 cut(s)	309, 911, 1052, 1193, 1367, 1691
BmrI	ACTGGG	2 cut(s)	1338, 1816
BmsI	GCATC	7 cut(s)	90, 289, 407, 889, 907, 2080, 2222
BmtI	GCTAGC	1 cut(s)	1466
BmuI	ACTGGG	2 cut(s)	1338, 1816
BpiI	GAAGAC	4 cut(s)	1053, 2043, 2066, 2199
BpmI	CTGGAG	2 cut(s)	738, 1073
Bpu1102I	GCTNAGC	1 cut(s)	290
BpuEI	CTTGAG	1 cut(s)	1774
BpuMI	CCSGG	1 cut(s)	1691
BsaJI	CCNNGG	2 cut(s)	546, 1191
BsaWI	WCCGGW	1 cut(s)	772
Bsc4I	CCNNNNNNNGG	2 cut(s)	1690, 2192
Bse1I	ACTGG	7 cut(s)	164, 678, 755, 1344, 1479, 1804, 1811
Bse3DI	GCAATG	1 cut(s)	829
BseBI	CCWGG	5 cut(s)	309, 911, 1052, 1193, 1367
BseDI	CCNNGG	2 cut(s)	546, 1191
BseGI	GGATG	6 cut(s)	196, 769, 934, 1462, 1774, 2095
BseLI	CCNNNNNNNGG	2 cut(s)	1690, 2192
BseMI	GCAATG	1 cut(s)	829
BseMII	CTCAG	3 cut(s)	239, 254, 2124
BseNI	ACTGG	7 cut(s)	164, 678, 755, 1344, 1479, 1804, 1811
BseSI	GKGCMC	2 cut(s)	1205, 1342
BseXI	GCAGC	6 cut(s)	26, 402, 529, 607, 907, 1894
BseYI	CCCAGC	1 cut(s)	2198
BsgI	GTGCAG	1 cut(s)	1896
BshFI	GGCC	1 cut(s)	72
BshNI	GGYRCC	1 cut(s)	1339
BsiHKAI	GWGCWC	2 cut(s)	835, 1119
BsiHKCI	CYCGRG	1 cut(s)	720
BsiSI	CCGG	2 cut(s)	773, 1691
BslFI	GGGAC	1 cut(s)	1637
BslI	CCNNNNNNNGG	2 cut(s)	1690, 2192
BsmAI	GTCTC	3 cut(s)	723, 810, 876
BsmFI	GGGAC	1 cut(s)	1637
BsmI	GAATGC	1 cut(s)	1842
BsnI	GGCC	1 cut(s)	72
BsoBI	CYCGRG	1 cut(s)	720
Bsp1286I	GDGCHC	5 cut(s)	835, 1119, 1205, 1342, 1416
Bsp1407I	TGTACA	2 cut(s)	1022, 1318
Bsp143I	GATC	6 cut(s)	243, 478, 840, 1693, 1793, 1914
Bsp1720I	GCTNAGC	1 cut(s)	290
Bsp19I	CCATGG	1 cut(s)	546
BspACI	CCGC	5 cut(s)	24, 27, 418, 1565, 2211
BspANI	GGCC	1 cut(s)	72
BspCNI	CTCAG	3 cut(s)	238, 255, 2125
BspHI	TCATGA	2 cut(s)	835, 1588
BspLI	GGNNCC	5 cut(s)	245, 558, 1341, 1665, 2166
BspMAI	CTGCAG	2 cut(s)	925, 1036
BspMI	ACCTGC	1 cut(s)	1025
BspOI	GCTAGC	1 cut(s)	1466
BspPI	GGATC	4 cut(s)	238, 251, 1701, 1788
BspT107I	GGYRCC	1 cut(s)	1339
BsrDI	GCAATG	1 cut(s)	829
BsrGI	TGTACA	2 cut(s)	1022, 1318
BsrI	ACTGG	7 cut(s)	164, 678, 755, 1344, 1479, 1804, 1811
BssECI	CCNNGG	2 cut(s)	546, 1191
BssMI	GATC	6 cut(s)	243, 478, 840, 1693, 1793, 1914
BssSI	CACGAG	1 cut(s)	1085
BssT1I	CCWWGG	1 cut(s)	546
Bst2BI	CACGAG	1 cut(s)	1085
Bst2UI	CCWGG	5 cut(s)	309, 911, 1052, 1193, 1367
Bst4CI	ACNGT	6 cut(s)	151, 532, 830, 1231, 1550, 2097
Bst6I	CTCTTC	2 cut(s)	742, 843
BstAPI	GCANNNNNTGC	1 cut(s)	899
BstAUI	TGTACA	2 cut(s)	1022, 1318
BstC8I	GCNNGC	4 cut(s)	105, 256, 1464, 2251
BstDEI	CTNAG	6 cut(s)	225, 263, 290, 934, 2133, 2191
BstDSI	CCRYGG	1 cut(s)	546
BstENI	CCTNNNNNAGG	1 cut(s)	2190
BstF5I	GGATG	6 cut(s)	196, 769, 934, 1462, 1774, 2095
BstH2I	RGCGCY	1 cut(s)	1712
BstHHI	GCGC	1 cut(s)	1711
BstKTI	GATC	6 cut(s)	246, 481, 843, 1696, 1796, 1917
BstMAI	GTCTC	3 cut(s)	723, 810, 876
BstMBI	GATC	6 cut(s)	243, 478, 840, 1693, 1793, 1914
BstMWI	GCNNNNNNNGC	6 cut(s)	36, 286, 604, 899, 920, 1509
BstNI	CCWGG	5 cut(s)	309, 911, 1052, 1193, 1367
BstNSI	RCATGY	4 cut(s)	142, 897, 1319, 1823
BstSCI	CCNGG	6 cut(s)	307, 909, 1050, 1191, 1365, 1689
BstSFI	CTRYAG	3 cut(s)	295, 921, 1032
BstSLI	GKGCMC	2 cut(s)	1205, 1342
BstV1I	GCAGC	6 cut(s)	26, 402, 529, 607, 907, 1894
BstV2I	GAAGAC	4 cut(s)	1053, 2043, 2066, 2199
BstX2I	RGATCY	4 cut(s)	243, 840, 1693, 1793
BstXI	CCANNNNNNTGG	1 cut(s)	553
BstYI	RGATCY	4 cut(s)	243, 840, 1693, 1793
BsuRI	GGCC	1 cut(s)	72
BtgI	CCRYGG	1 cut(s)	546
BtgZI	GCGATG	1 cut(s)	327
BtsCI	GGATG	6 cut(s)	196, 769, 934, 1462, 1774, 2095
BtsI	GCAGTG	3 cut(s)	372, 930, 1497
BveI	ACCTGC	1 cut(s)	1025
Cac8I	GCNNGC	4 cut(s)	105, 256, 1464, 2251
CciI	TCATGA	2 cut(s)	835, 1588
CfoI	GCGC	1 cut(s)	1711
Cfr13I	GGNCC	3 cut(s)	556, 1355, 2164
Csp6I	GTAC	3 cut(s)	1023, 1227, 1319
CviQI	GTAC	3 cut(s)	1023, 1227, 1319
DdeI	CTNAG	6 cut(s)	225, 263, 290, 934, 2133, 2191
DpnI	GATC	6 cut(s)	245, 480, 842, 1695, 1795, 1916
DpnII	GATC	6 cut(s)	243, 478, 840, 1693, 1793, 1914
EaeI	YGGCCR	1 cut(s)	70
Eam1104I	CTCTTC	2 cut(s)	742, 843
EarI	CTCTTC	2 cut(s)	742, 843
Eco130I	CCWWGG	1 cut(s)	546
Eco24I	GRGCYC	1 cut(s)	1416
Eco32I	GATATC	1 cut(s)	2227
Eco47I	GGWCC	3 cut(s)	556, 1355, 2164
Eco57I	CTGAAG	2 cut(s)	1085, 1674
Eco88I	CYCGRG	1 cut(s)	720
EcoNI	CCTNNNNNAGG	1 cut(s)	2190
EcoO109I	RGGNCCY	1 cut(s)	2164
EcoRII	CCWGG	5 cut(s)	307, 909, 1050, 1191, 1365
EcoRV	GATATC	1 cut(s)	2227
EcoT14I	CCWWGG	1 cut(s)	546
EcoT22I	ATGCAT	2 cut(s)	1270, 2119
EcoT38I	GRGCYC	1 cut(s)	1416
ErhI	CCWWGG	1 cut(s)	546
FalI	AAGNNNNNCTT	6 cut(s)	1315, 1347, 1593, 1625, 1695, 1727
FaqI	GGGAC	1 cut(s)	1637
FauI	CCCGC	1 cut(s)	411
FauNDI	CATATG	1 cut(s)	1174
Fnu4HI	GCNGC	8 cut(s)	25, 28, 40, 416, 543, 596, 921, 1908
FokI	GGATG	6 cut(s)	203, 756, 941, 1469, 1781, 2102
FriOI	GRGCYC	1 cut(s)	1416
Fsp4HI	GCNGC	8 cut(s)	25, 28, 40, 416, 543, 596, 921, 1908
FspBI	CTAG	5 cut(s)	510, 1283, 1463, 2070, 2180
GlaI	GCGC	1 cut(s)	1710
GluI	GCNGC	8 cut(s)	25, 28, 40, 416, 543, 596, 921, 1908
GsaI	CCCAGC	1 cut(s)	2202
GsuI	CTGGAG	2 cut(s)	738, 1073
HaeII	RGCGCY	1 cut(s)	1712
HaeIII	GGCC	1 cut(s)	72
HapII	CCGG	2 cut(s)	773, 1691
HhaI	GCGC	1 cut(s)	1711
Hin6I	GCGC	1 cut(s)	1709
HinP1I	GCGC	1 cut(s)	1709
HincII	GTYRAC	3 cut(s)	669, 1543, 2109
HindII	GTYRAC	3 cut(s)	669, 1543, 2109
HindIII	AAGCTT	3 cut(s)	287, 1501, 1602
HpaI	GTTAAC	1 cut(s)	669
HpaII	CCGG	2 cut(s)	773, 1691
HphI	GGTGA	3 cut(s)	171, 656, 2224
Hpy166II	GTNNAC	5 cut(s)	121, 669, 1543, 2109, 2240
Hpy8I	GTNNAC	5 cut(s)	121, 669, 1543, 2109, 2240
HpyCH4III	ACNGT	6 cut(s)	151, 532, 830, 1231, 1550, 2097
HpyCH4IV	ACGT	1 cut(s)	665
HpyF10VI	GCNNNNNNNGC	6 cut(s)	36, 286, 604, 899, 920, 1509
HpyF3I	CTNAG	6 cut(s)	225, 263, 290, 934, 2133, 2191
HpySE526I	ACGT	1 cut(s)	665
HspAI	GCGC	1 cut(s)	1709
KspAI	GTTAAC	1 cut(s)	669
Kzo9I	GATC	6 cut(s)	243, 478, 840, 1693, 1793, 1914
LmnI	GCTCC	2 cut(s)	495, 757
Lsp1109I	GCAGC	6 cut(s)	26, 402, 529, 607, 907, 1894
LweI	GCATC	7 cut(s)	90, 289, 407, 889, 907, 2080, 2222
MaeI	CTAG	5 cut(s)	510, 1283, 1463, 2070, 2180
MaeII	ACGT	1 cut(s)	665
MaeIII	GTNAC	6 cut(s)	134, 370, 1450, 1802, 1919, 2080
MalI	GATC	6 cut(s)	245, 480, 842, 1695, 1795, 1916
MboI	GATC	6 cut(s)	243, 478, 840, 1693, 1793, 1914
MflI	RGATCY	4 cut(s)	243, 840, 1693, 1793
MhlI	GDGCHC	5 cut(s)	835, 1119, 1205, 1342, 1416
MlyI	GAGTC	5 cut(s)	298, 725, 812, 1619, 2077
MmeI	TCCRAC	2 cut(s)	534, 1551
MnlI	CCTC	9 cut(s)	71, 167, 571, 644, 693, 1048, 1431, 1439, 2046
Mph1103I	ATGCAT	2 cut(s)	1270, 2119
MroXI	GAANNNNTTC	1 cut(s)	1648
MslI	CAYNNNNRTG	3 cut(s)	466, 892, 1532
MspA1I	CMGCKG	3 cut(s)	39, 107, 1567
MspI	CCGG	2 cut(s)	773, 1691
MspR9I	CCNGG	6 cut(s)	309, 911, 1052, 1193, 1367, 1691
Mva1269I	GAATGC	1 cut(s)	1842
MvaI	CCWGG	5 cut(s)	309, 911, 1052, 1193, 1367
MwoI	GCNNNNNNNGC	6 cut(s)	36, 286, 604, 899, 920, 1509
NciI	CCSGG	1 cut(s)	1691
NcoI	CCATGG	1 cut(s)	546
NdeI	CATATG	1 cut(s)	1174
NdeII	GATC	6 cut(s)	243, 478, 840, 1693, 1793, 1914
NheI	GCTAGC	1 cut(s)	1462
NlaIV	GGNNCC	5 cut(s)	245, 558, 1341, 1665, 2166
NmuCI	GTSAC	4 cut(s)	370, 1450, 1919, 2080
NsiI	ATGCAT	2 cut(s)	1270, 2119
NspI	RCATGY	4 cut(s)	142, 897, 1319, 1823
PaeR7I	CTCGAG	1 cut(s)	720
PagI	TCATGA	2 cut(s)	835, 1588
PciI	ACATGT	1 cut(s)	138
PctI	GAATGC	1 cut(s)	1842
PdmI	GAANNNNTTC	1 cut(s)	1648
PfeI	GAWTC	8 cut(s)	113, 361, 702, 743, 1016, 1076, 1835, 2140
PfoI	TCCNGGA	1 cut(s)	1689
PkrI	GCNGC	8 cut(s)	26, 29, 41, 417, 544, 597, 922, 1909
PleI	GAGTC	5 cut(s)	298, 724, 811, 1619, 2077
PpsI	GAGTC	5 cut(s)	298, 724, 811, 1619, 2077
PpuMI	RGGWCCY	1 cut(s)	2164
PscI	ACATGT	1 cut(s)	138
PshBI	ATTAAT	1 cut(s)	633
Psp1406I	AACGTT	1 cut(s)	665
Psp5II	RGGWCCY	1 cut(s)	2164
Psp6I	CCWGG	5 cut(s)	307, 909, 1050, 1191, 1365
PspFI	CCCAGC	1 cut(s)	2198
PspGI	CCWGG	5 cut(s)	307, 909, 1050, 1191, 1365
PspN4I	GGNNCC	5 cut(s)	245, 558, 1341, 1665, 2166
PspPI	GGNCC	3 cut(s)	556, 1355, 2164
PspPPI	RGGWCCY	1 cut(s)	2164
PsrI	GAACNNNNNNTAC	2 cut(s)	2039, 2071
PstI	CTGCAG	2 cut(s)	925, 1036
PsuI	RGATCY	4 cut(s)	243, 840, 1693, 1793
PvuII	CAGCTG	2 cut(s)	39, 107
RsaI	GTAC	3 cut(s)	1024, 1228, 1320
RsaNI	GTAC	3 cut(s)	1023, 1227, 1319
RseI	CAYNNNNRTG	3 cut(s)	466, 892, 1532
SatI	GCNGC	8 cut(s)	25, 28, 40, 416, 543, 596, 921, 1908
Sau3AI	GATC	6 cut(s)	243, 478, 840, 1693, 1793, 1914
Sau96I	GGNCC	3 cut(s)	556, 1355, 2164
SchI	GAGTC	5 cut(s)	298, 725, 812, 1619, 2077
ScrFI	CCNGG	6 cut(s)	309, 911, 1052, 1193, 1367, 1691
SduI	GDGCHC	5 cut(s)	835, 1119, 1205, 1342, 1416
SfaNI	GCATC	7 cut(s)	90, 289, 407, 889, 907, 2080, 2222
SfcI	CTRYAG	3 cut(s)	295, 921, 1032
Sfr274I	CTCGAG	1 cut(s)	720
SinI	GGWCC	3 cut(s)	556, 1355, 2164
SlaI	CTCGAG	1 cut(s)	720
SmiMI	CAYNNNNRTG	3 cut(s)	466, 892, 1532
SmlI	CTYRAG	2 cut(s)	720, 1789
SmoI	CTYRAG	2 cut(s)	720, 1789
SpeI	ACTAGT	2 cut(s)	509, 2069
SsiI	CCGC	5 cut(s)	24, 27, 418, 1565, 2211
SspMI	CTAG	5 cut(s)	510, 1283, 1463, 2070, 2180
StyD4I	CCNGG	6 cut(s)	307, 909, 1050, 1191, 1365, 1689
StyI	CCWWGG	1 cut(s)	546
TaaI	ACNGT	6 cut(s)	151, 532, 830, 1231, 1550, 2097
TaiI	ACGT	1 cut(s)	668
TaqI	TCGA	4 cut(s)	321, 700, 721, 1416
TatI	WGTACW	3 cut(s)	1022, 1226, 1318
TauI	GCSGC	2 cut(s)	27, 30
TfiI	GAWTC	8 cut(s)	113, 361, 702, 743, 1016, 1076, 1835, 2140
TseFI	GTSAC	4 cut(s)	370, 1450, 1919, 2080
TseI	GCWGC	6 cut(s)	39, 415, 542, 595, 920, 1907
Tsp45I	GTSAC	4 cut(s)	370, 1450, 1919, 2080
TspGWI	ACGGA	1 cut(s)	1469
VpaK11BI	GGWCC	3 cut(s)	556, 1355, 2164
VspI	ATTAAT	1 cut(s)	633
XagI	CCTNNNNNAGG	1 cut(s)	2190
XapI	RAATTY	5 cut(s)	94, 219, 1560, 1945, 2126
XceI	RCATGY	4 cut(s)	142, 897, 1319, 1823
XhoI	CTCGAG	1 cut(s)	720
XmnI	GAANNNNTTC	1 cut(s)	1648
XspI	CTAG	5 cut(s)	510, 1283, 1463, 2070, 2180
Zsp2I	ATGCAT	2 cut(s)	1270, 2119

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.