Rw2G033330

Diphthamide synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002016
Physical Location & Seq
Forward (+)
54619227 .. 54621689
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033330.1

Sequence Viewer

Length: 522 bp
ATGTTAAGATTTTTCAAGAGTTTCTGGTCCTTGAGAAGGATGGAATCTGGAAATAAAGAGTCTCAAAAGAAAAGTTCACTGGAAGAGTTTGATTCTGAGATTATTGCTGTGGCAAATTTGATGCTAGCTGATGATTCGGTGGACGAGCTTGATAGTTACATGTATCAAACTGTTGGGCACCAGATAGTTATCAGCTATGCAGAATGCATGGGAGTGCCATTGTTCAGAAGGCAAATACAAGGATCCACAAGGCATCAGAAGCTTAGCTATGGAATGACTCCTGGCGATGAAGTCGAAGATGTGTTTATTTTGTTAAATGAAGTGAAACGACAGATTCCTTCTGTCACTGCTGTATCGTCTGGTGCTATTGCATCAGACTACCAAAGGTTGTGGGTGGAAAGTGTGTGTTCAAGGTTGGGTCTTGTTTCTGTAGCATACTTGTGGAAACAAGATCAGTCATTGCTCCTTCAAGAAATGATAACTAGTGGGATTGTGGCGAACAGTGAAGTGAATTTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Diphthami_syn_2 Pkinase Ribonuc_L-PSP

Protein Analysis

173

Amino Acids

19.55

Weight (kDa)

4.95

Isoelectric Point (pI)

59.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthami_syn_2 PF01902 48 - 163 1.1e-14 Diphthamide synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 177
AclWI GGATC 2 cut(s) 237, 250
AcsI RAATTY 2 cut(s) 115, 511
AfiI CCNNNNNNNGG 1 cut(s) 36
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 3 cut(s) 16, 411, 470
AhlI ACTAGT 1 cut(s) 482
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 5 cut(s) 128, 148, 195, 262, 267
AluI AGCT 5 cut(s) 128, 148, 195, 262, 267
Alw26I GTCTC 1 cut(s) 66
AlwI GGATC 2 cut(s) 237, 250
ApoI RAATTY 2 cut(s) 115, 511
AspS9I GGNCC 1 cut(s) 27
AsuNHI GCTAGC 1 cut(s) 124
AvaII GGWCC 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 180
BaeI ACNNNNGTAYC 2 cut(s) 336, 369
BamHI GGATCC 1 cut(s) 242
BanI GGYRCC 1 cut(s) 177
BccI CCATC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 66
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 2 cut(s) 125, 483
BfmI CTRYAG 1 cut(s) 429
BlpI GCTNAGC 1 cut(s) 263
Bme1390I CCNGG 1 cut(s) 282
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 2 cut(s) 179, 244
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 111, 262, 380
BmtI GCTAGC 1 cut(s) 128
Bpu1102I GCTNAGC 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 52
BsaBI GATNNNNATC 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 84
Bse3DI GCAATG 1 cut(s) 458
Bse8I GATNNNNATC 1 cut(s) 188
BseBI CCWGG 1 cut(s) 282
BseGI GGATG 1 cut(s) 45
BseJI GATNNNNATC 1 cut(s) 188
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMI GCAATG 1 cut(s) 458
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 84
BseSI GKGCMC 1 cut(s) 180
BshNI GGYRCC 1 cut(s) 177
BslI CCNNNNNNNGG 1 cut(s) 36
BsmAI GTCTC 1 cut(s) 66
BsmI GAATGC 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 2 cut(s) 242, 451
Bsp1720I GCTNAGC 1 cut(s) 263
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 2 cut(s) 179, 244
BspOI GCTAGC 1 cut(s) 128
BspPI GGATC 2 cut(s) 237, 250
BspT107I GGYRCC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 458
BsrI ACTGG 1 cut(s) 84
BssMI GATC 2 cut(s) 242, 451
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 172, 503
Bst6I CTCTTC 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 96, 263
BstENI CCTNNNNNAGG 1 cut(s) 34
BstF5I GGATG 1 cut(s) 45
BstKTI GATC 2 cut(s) 245, 454
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 2 cut(s) 242, 451
BstMWI GCNNNNNNNGC 1 cut(s) 259
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 280
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BtgZI GCGATG 1 cut(s) 300
BtsCI GGATG 1 cut(s) 45
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 3 cut(s) 77, 345, 508
Cac8I GCNNGC 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 27
CspCI CAANNNNNGTGG 2 cut(s) 371, 406
CviAII CATG 2 cut(s) 160, 208
CviJI RGCY 5 cut(s) 128, 148, 195, 262, 267
CviKI_1 RGCY 5 cut(s) 128, 148, 195, 262, 267
DdeI CTNAG 2 cut(s) 96, 263
DpnI GATC 2 cut(s) 244, 453
DpnII GATC 2 cut(s) 242, 451
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 27
EcoNI CCTNNNNNAGG 1 cut(s) 34
EcoRII CCWGG 1 cut(s) 280
EcoT22I ATGCAT 1 cut(s) 209
FaeI CATG 2 cut(s) 163, 211
FaiI YATR 5 cut(s) 161, 198, 209, 270, 436
FatI CATG 2 cut(s) 159, 207
FokI GGATG 1 cut(s) 52
FspBI CTAG 2 cut(s) 125, 483
Hin1II CATG 2 cut(s) 163, 211
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 6 cut(s) 44, 59, 92, 134, 277, 334
Hpy166II GTNNAC 2 cut(s) 77, 142
Hpy188I TCNGA 4 cut(s) 97, 227, 258, 376
Hpy188III TCNNGA 3 cut(s) 16, 48, 470
Hpy8I GTNNAC 2 cut(s) 77, 142
HpyAV CCTTC 4 cut(s) 30, 222, 348, 476
HpyCH4III ACNGT 2 cut(s) 172, 503
HpyCH4V TGCA 3 cut(s) 200, 207, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 259
HpyF3I CTNAG 2 cut(s) 96, 263
Hsp92II CATG 2 cut(s) 163, 211
Kzo9I GATC 2 cut(s) 242, 451
LmnI GCTCC 1 cut(s) 468
LpnPI CCDG 7 cut(s) 10, 33, 65, 194, 267, 294, 345
LweI GCATC 3 cut(s) 111, 262, 380
MaeI CTAG 2 cut(s) 125, 483
MaeIII GTNAC 2 cut(s) 155, 343
MalI GATC 2 cut(s) 244, 453
MboI GATC 2 cut(s) 242, 451
MboII GAAGA 2 cut(s) 95, 308
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 2 cut(s) 115, 511
MlyI GAGTC 2 cut(s) 68, 271
Mph1103I ATGCAT 1 cut(s) 209
MseI TTAA 3 cut(s) 5, 314, 520
MslI CAYNNNNRTG 2 cut(s) 212, 439
MspR9I CCNGG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 209
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 259
NdeII GATC 2 cut(s) 242, 451
NheI GCTAGC 1 cut(s) 124
NlaIII CATG 2 cut(s) 163, 211
NlaIV GGNNCC 2 cut(s) 179, 244
NmuCI GTSAC 1 cut(s) 343
NsiI ATGCAT 1 cut(s) 209
NspI RCATGY 1 cut(s) 163
PciI ACATGT 1 cut(s) 159
PctI GAATGC 1 cut(s) 209
PfeI GAWTC 4 cut(s) 44, 92, 134, 334
PleI GAGTC 2 cut(s) 67, 271
PpsI GAGTC 2 cut(s) 67, 271
PscI ACATGT 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 179, 244
PspPI GGNCC 1 cut(s) 27
PsuI RGATCY 1 cut(s) 242
RseI CAYNNNNRTG 2 cut(s) 212, 439
SaqAI TTAA 3 cut(s) 5, 314, 520
Sau3AI GATC 2 cut(s) 242, 451
Sau96I GGNCC 1 cut(s) 27
SchI GAGTC 2 cut(s) 68, 271
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 1 cut(s) 180
SetI ASST 7 cut(s) 130, 150, 197, 264, 269, 389, 416
SfaNI GCATC 3 cut(s) 111, 262, 380
SfcI CTRYAG 1 cut(s) 429
SinI GGWCC 1 cut(s) 27
SmiMI CAYNNNNRTG 2 cut(s) 212, 439
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
SpeI ACTAGT 1 cut(s) 482
Sse9I AATT 2 cut(s) 115, 511
SspMI CTAG 2 cut(s) 125, 483
StyD4I CCNGG 1 cut(s) 280
TaaI ACNGT 2 cut(s) 172, 503
TaqI TCGA 1 cut(s) 294
TasI AATT 2 cut(s) 115, 511
TfiI GAWTC 4 cut(s) 44, 92, 134, 334
Tru1I TTAA 3 cut(s) 5, 314, 520
Tru9I TTAA 3 cut(s) 5, 314, 520
TscAI CASTG 3 cut(s) 84, 352, 508
TseFI GTSAC 1 cut(s) 343
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 2 cut(s) 303, 333
TspRI CASTG 3 cut(s) 84, 352, 508
VpaK11BI GGWCC 1 cut(s) 27
XagI CCTNNNNNAGG 1 cut(s) 34
XapI RAATTY 2 cut(s) 115, 511
XceI RCATGY 1 cut(s) 163
XspI CTAG 2 cut(s) 125, 483
Zsp2I ATGCAT 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.