Rh2BG670500

Diphthine--ammonia

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002016
Physical Location & Seq
Reverse (-)
88249420 .. 88254205
4786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG670500.1

Sequence Viewer

Length: 768 bp
ATGAATGAAGATGAAAAGGAACTCTTTATTGTTGCTGTGGCAAATTTGACGCCAGCTGATGATTCGGTGGACGAGCTTGATAGTTACATGTATCAAACTATAAGTCTCCTTCCTCTTGTCTATATTTCACCTTATTTGAATGTTTCTTCTTTCAAAAGCAAAGAGTTAAGTTCTGTATGTTGGGCACCAGATAGTTATCAGCTATGCAGATTGCATGGGAGTGCCATTGTTCAGAAGGCGAATACAAGGATCCACAAGGCAAGCCTTCTGAGAAGGGTATTTCGGCATCAAAAGCTTAGCTATAGAATGACTCCTGGTGATGAAGTCGAAGATATGTTTATTTTGTTAAATGAAGTGAAACGACAGATTCCTTCTGTCACTGCTGTATCTTCTGGTGCTATTGCATCAGACTACCAAAGGTTGCGGGTGGAAAGTGTCTGTTCAAGGTTAGGTCTTGTTTCTCTAGCATCCTTGTGGAAACAAGGTCAGTCATTGCTCCTTCAAGAAATGATAACTAGTGGGATTGTGGCGATAACAGTGAAGGTCGACTACTCTCTTCTTTCCAGTTGCAAATCCAGAATCTGGCGCAACAACCTACCTCTGTTCGTCAGGTTTCGGCCCACGGCAGGAAGCGTGCACAACGCTATTGTCAACGACAGGAAGCTCGTTGATGTGGTTAAGAGGATAGGAAGCTCGTCAACAGGAATCGGGGCTGGACCTTGTCAATCGGAGTCGAAATCGAAATCTCAGCTGGGCCTTGCCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Diphthami_syn_2 Pkinase Ribonuc_L-PSP

Protein Analysis

255

Amino Acids

28.24

Weight (kDa)

9.19

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthami_syn_2 PF01902 107 - 185 2.8e-10 Diphthamide synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 582
AccI GTMKAC 1 cut(s) 546
AciI CCGC 1 cut(s) 424
AclWI GGATC 2 cut(s) 244, 257
AcsI RAATTY 1 cut(s) 43
AcyI GRCGYC 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 582, 626
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 4 cut(s) 139, 154, 444, 503
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 8 cut(s) 56, 76, 202, 295, 300, 664, 693, 751
AluI AGCT 8 cut(s) 56, 76, 202, 295, 300, 664, 693, 751
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 1 cut(s) 110
Alw44I GTGCAC 1 cut(s) 635
AlwI GGATC 2 cut(s) 244, 257
AlwNI CAGNNNCTG 1 cut(s) 582
AoxI GGCC 2 cut(s) 617, 754
ApaLI GTGCAC 1 cut(s) 635
ApoI RAATTY 1 cut(s) 43
ArsI GACNNNNNNTTYG 2 cut(s) 88, 120
AspLEI GCGC 1 cut(s) 588
AspS9I GGNCC 3 cut(s) 618, 716, 754
AsuHPI GGTGA 2 cut(s) 120, 329
AvaII GGWCC 1 cut(s) 716
BaeGI GKGCMC 2 cut(s) 187, 639
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BamHI GGATCC 1 cut(s) 249
BanI GGYRCC 1 cut(s) 184
BarI GAAGNNNNNNTAC 2 cut(s) 533, 565
Bbv12I GWGCWC 1 cut(s) 639
BceAI ACGGC 2 cut(s) 639, 746
BciT130I CCWGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 110
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 3 cut(s) 464, 516, 766
BfmI CTRYAG 1 cut(s) 301
BlpI GCTNAGC 1 cut(s) 296
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 716
BmgT120I GGNCC 3 cut(s) 618, 716, 754
BmiI GGNNCC 2 cut(s) 186, 251
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 3 cut(s) 295, 413, 476
Bpu1102I GCTNAGC 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 621
Bsc4I CCNNNNNNNGG 2 cut(s) 582, 626
Bse1I ACTGG 1 cut(s) 564
Bse3DI GCAATG 1 cut(s) 491
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 621
BseGI GGATG 1 cut(s) 467
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 2 cut(s) 582, 626
BseMI GCAATG 1 cut(s) 491
BseMII CTCAG 2 cut(s) 260, 761
BseNI ACTGG 1 cut(s) 564
BseSI GKGCMC 2 cut(s) 187, 639
BseYI CCCAGC 1 cut(s) 751
BshFI GGCC 2 cut(s) 619, 756
BshNI GGYRCC 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 639
BslI CCNNNNNNNGG 2 cut(s) 582, 626
BsmAI GTCTC 1 cut(s) 110
BsnI GGCC 2 cut(s) 619, 756
Bsp1286I GDGCHC 2 cut(s) 187, 639
Bsp143I GATC 1 cut(s) 249
Bsp1720I GCTNAGC 1 cut(s) 296
BspACI CCGC 1 cut(s) 424
BspANI GGCC 2 cut(s) 619, 756
BspCNI CTCAG 2 cut(s) 261, 760
BspLI GGNNCC 2 cut(s) 186, 251
BspPI GGATC 2 cut(s) 244, 257
BspT107I GGYRCC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 491
BsrI ACTGG 1 cut(s) 564
BssECI CCNNGG 1 cut(s) 621
BssMI GATC 1 cut(s) 249
BssNI GRCGYC 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 538
Bst6I CTCTTC 1 cut(s) 561
BstACI GRCGYC 1 cut(s) 50
BstC8I GCNNGC 3 cut(s) 54, 262, 635
BstDEI CTNAG 3 cut(s) 269, 296, 747
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 1 cut(s) 467
BstHHI GCGC 1 cut(s) 588
BstKTI GATC 1 cut(s) 252
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 249
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 301
BstSLI GKGCMC 2 cut(s) 187, 639
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
BsuRI GGCC 2 cut(s) 619, 756
BtgI CCRYGG 1 cut(s) 621
BtsCI GGATG 1 cut(s) 467
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 2 cut(s) 378, 543
Cac8I GCNNGC 3 cut(s) 54, 262, 635
CaiI CAGNNNCTG 1 cut(s) 582
CfoI GCGC 1 cut(s) 588
Cfr13I GGNCC 3 cut(s) 618, 716, 754
CseI GACGC 1 cut(s) 58
CviAII CATG 2 cut(s) 88, 215
DdeI CTNAG 3 cut(s) 269, 296, 747
DpnI GATC 1 cut(s) 251
DpnII GATC 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 716
EcoRII CCWGG 1 cut(s) 313
FaeI CATG 2 cut(s) 91, 218
FaiI YATR 8 cut(s) 89, 101, 123, 178, 205, 216, 303, 335
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FatI CATG 2 cut(s) 87, 214
FauI CCCGC 1 cut(s) 417
FblI GTMKAC 1 cut(s) 546
FokI GGATG 1 cut(s) 454
FspBI CTAG 3 cut(s) 464, 516, 766
GlaI GCGC 1 cut(s) 587
GsaI CCCAGC 1 cut(s) 755
HaeIII GGCC 2 cut(s) 619, 756
HgaI GACGC 1 cut(s) 58
HhaI GCGC 1 cut(s) 588
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 2 cut(s) 91, 218
Hin6I GCGC 1 cut(s) 586
HinP1I GCGC 1 cut(s) 586
HincII GTYRAC 3 cut(s) 547, 652, 699
HindII GTYRAC 3 cut(s) 547, 652, 699
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 6 cut(s) 62, 310, 367, 579, 705, 731
HphI GGTGA 2 cut(s) 120, 329
Hpy166II GTNNAC 5 cut(s) 70, 547, 637, 652, 699
Hpy188I TCNGA 4 cut(s) 234, 270, 409, 730
Hpy188III TCNNGA 2 cut(s) 503, 576
Hpy8I GTNNAC 5 cut(s) 70, 547, 637, 652, 699
HpyAV CCTTC 7 cut(s) 119, 229, 267, 275, 381, 509, 535
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4V TGCA 5 cut(s) 207, 214, 404, 570, 637
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
HpyF3I CTNAG 3 cut(s) 269, 296, 747
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 2 cut(s) 91, 218
HspAI GCGC 1 cut(s) 586
Kzo9I GATC 1 cut(s) 249
LmnI GCTCC 1 cut(s) 501
LweI GCATC 3 cut(s) 295, 413, 476
MaeI CTAG 3 cut(s) 464, 516, 766
MaeIII GTNAC 2 cut(s) 83, 376
MalI GATC 1 cut(s) 251
MboI GATC 1 cut(s) 249
MboII GAAGA 5 cut(s) 20, 138, 341, 381, 548
MflI RGATCY 1 cut(s) 249
MhlI GDGCHC 2 cut(s) 187, 639
MluCI AATT 1 cut(s) 43
MlyI GAGTC 2 cut(s) 304, 740
MnlI CCTC 3 cut(s) 123, 609, 675
MseI TTAA 3 cut(s) 167, 347, 678
MslI CAYNNNNRTG 2 cut(s) 219, 472
MspA1I CMGCKG 2 cut(s) 56, 751
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 292
NdeII GATC 1 cut(s) 249
NlaIII CATG 2 cut(s) 91, 218
NlaIV GGNNCC 2 cut(s) 186, 251
NmuCI GTSAC 1 cut(s) 376
NspI RCATGY 1 cut(s) 91
PciI ACATGT 1 cut(s) 87
PfeI GAWTC 4 cut(s) 62, 367, 579, 705
PflFI GACNNNGTC 1 cut(s) 720
PflMI CCANNNNNTGG 1 cut(s) 582
PleI GAGTC 2 cut(s) 304, 739
PpsI GAGTC 2 cut(s) 304, 739
PscI ACATGT 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 751
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 2 cut(s) 186, 251
PspPI GGNCC 3 cut(s) 618, 716, 754
PstNI CAGNNNCTG 1 cut(s) 582
PsuI RGATCY 1 cut(s) 249
PsyI GACNNNGTC 1 cut(s) 720
PvuII CAGCTG 2 cut(s) 56, 751
RseI CAYNNNNRTG 2 cut(s) 219, 472
SalI GTCGAC 1 cut(s) 545
SaqAI TTAA 3 cut(s) 167, 347, 678
Sau3AI GATC 1 cut(s) 249
Sau96I GGNCC 3 cut(s) 618, 716, 754
SchI GAGTC 2 cut(s) 304, 740
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 2 cut(s) 187, 639
SfaNI GCATC 3 cut(s) 295, 413, 476
SfcI CTRYAG 1 cut(s) 301
SinI GGWCC 1 cut(s) 716
SmiMI CAYNNNNRTG 2 cut(s) 219, 472
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 1 cut(s) 43
SsiI CCGC 1 cut(s) 424
SspMI CTAG 3 cut(s) 464, 516, 766
StyD4I CCNGG 1 cut(s) 313
TaaI ACNGT 1 cut(s) 538
TaqI TCGA 4 cut(s) 327, 546, 734, 740
TasI AATT 1 cut(s) 43
TfiI GAWTC 4 cut(s) 62, 367, 579, 705
Tru1I TTAA 3 cut(s) 167, 347, 678
Tru9I TTAA 3 cut(s) 167, 347, 678
TscAI CASTG 2 cut(s) 385, 543
TseFI GTSAC 1 cut(s) 376
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 5 cut(s) 17, 21, 27, 336, 366
TspRI CASTG 2 cut(s) 385, 543
Tth111I GACNNNGTC 1 cut(s) 720
Van91I CCANNNNNTGG 1 cut(s) 582
VneI GTGCAC 1 cut(s) 635
VpaK11BI GGWCC 1 cut(s) 716
XapI RAATTY 1 cut(s) 43
XceI RCATGY 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 546
XspI CTAG 3 cut(s) 464, 516, 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.