RLG00000036278

Diphthamide synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002016
Physical Location & Seq
Forward (+)
78356507 .. 78358899
2393 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036278

Sequence Viewer

Length: 474 bp
ATGGCCATCAGAAGCGTCGATTTCAAGTGCTGGAATGGATTTATTGTTGCTGTGGCAAATTTGACGCCAGCTGCTGATTTGGTGGACGAGCTTGATAGTTACATGTATCAAACTGTAAGTCTCCTTCCTCTTGTCTATATTTCACCTTATTTGAATGTTTCTTCTTTCAAAAGCAAAGAGTTAAGTTCTGTATGTATGTATCTATGCAGAATGCATGGGAGTGCCATTGTTCAGAAGGCGAATTCAAGGATCCACAAGGCAAGCCTTCTGAGAAGGGTATTTTGGCATCAGAAGCTTAGCTATAGAATGACTCCTGGCGATGGTATCGAAGATATGTTTATTTTGTTAAATGAAGTGAAACGACAGATTCTTTCTGTCACTGCTGTATCTTCTGGTGCTATTGCATCAGACTACCAAAGGCTGCGGGTGGAAAGTGTCTGTTCAAGGTTAGGTCTTGTTTCTCTAGCATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Diphthami_syn_2 Pkinase Ribonuc_L-PSP

Protein Analysis

158

Amino Acids

17.65

Weight (kDa)

9.12

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 424
AclWI GGATC 2 cut(s) 244, 257
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 58, 241
AcyI GRCGYC 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 320
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 5 cut(s) 25, 154, 169, 246, 444
AjnI CCWGG 1 cut(s) 313
AjuI GAANNNNNNNTTGG 2 cut(s) 265, 297
AluBI AGCT 4 cut(s) 71, 91, 295, 300
AluI AGCT 4 cut(s) 71, 91, 295, 300
Alw26I GTCTC 1 cut(s) 125
AlwI GGATC 2 cut(s) 244, 257
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 71, 421
ApoI RAATTY 2 cut(s) 58, 241
ArsI GACNNNNNNTTYG 2 cut(s) 103, 135
AsuHPI GGTGA 1 cut(s) 135
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 249
BarI GAAGNNNNNNTAC 2 cut(s) 108, 140
BbvI GCAGC 2 cut(s) 58, 408
BccI CCATC 2 cut(s) 14, 314
BciT130I CCWGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 125
BfaI CTAG 2 cut(s) 464, 472
BfmI CTRYAG 1 cut(s) 301
BisI GCNGC 2 cut(s) 72, 422
BlpI GCTNAGC 1 cut(s) 296
BlsI GCNGC 2 cut(s) 73, 423
Bme1390I CCNGG 1 cut(s) 315
BmiI GGNNCC 1 cut(s) 251
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 2 cut(s) 295, 413
Bpu1102I GCTNAGC 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 65
Bsc4I CCNNNNNNNGG 1 cut(s) 320
BseBI CCWGG 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 320
BseMII CTCAG 1 cut(s) 260
BseXI GCAGC 2 cut(s) 58, 408
BshFI GGCC 1 cut(s) 5
BslI CCNNNNNNNGG 1 cut(s) 320
BsmAI GTCTC 1 cut(s) 125
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 249
Bsp1720I GCTNAGC 1 cut(s) 296
BspACI CCGC 1 cut(s) 424
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 261
BspLI GGNNCC 1 cut(s) 251
BspPI GGATC 2 cut(s) 244, 257
BssMI GATC 1 cut(s) 249
BssNI GRCGYC 1 cut(s) 65
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 115
BstACI GRCGYC 1 cut(s) 65
BstC8I GCNNGC 2 cut(s) 69, 262
BstDEI CTNAG 2 cut(s) 269, 296
BstKTI GATC 1 cut(s) 252
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 1 cut(s) 249
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 301
BstV1I GCAGC 2 cut(s) 58, 408
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 333
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 2 cut(s) 69, 262
CaiI CAGNNNCTG 1 cut(s) 74
CseI GACGC 2 cut(s) 4, 73
CviAII CATG 2 cut(s) 103, 215
CviJI RGCY 7 cut(s) 5, 71, 91, 264, 295, 300, 421
CviKI_1 RGCY 7 cut(s) 5, 71, 91, 264, 295, 300, 421
DdeI CTNAG 2 cut(s) 269, 296
DpnI GATC 1 cut(s) 251
DpnII GATC 1 cut(s) 249
EaeI YGGCCR 1 cut(s) 3
EcoRI GAATTC 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 313
EcoT22I ATGCAT 1 cut(s) 216
FaeI CATG 2 cut(s) 106, 218
FaiI YATR 9 cut(s) 104, 138, 193, 197, 205, 216, 303, 335, 469
FatI CATG 2 cut(s) 102, 214
FauI CCCGC 1 cut(s) 417
Fnu4HI GCNGC 2 cut(s) 72, 422
Fsp4HI GCNGC 2 cut(s) 72, 422
FspBI CTAG 2 cut(s) 464, 472
GluI GCNGC 2 cut(s) 72, 422
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 2 cut(s) 4, 73
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 2 cut(s) 106, 218
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 2 cut(s) 310, 367
HphI GGTGA 1 cut(s) 135
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 5 cut(s) 11, 234, 270, 291, 409
Hpy8I GTNNAC 1 cut(s) 85
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 4 cut(s) 134, 229, 267, 275
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4V TGCA 3 cut(s) 207, 214, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
HpyF3I CTNAG 2 cut(s) 269, 296
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 2 cut(s) 106, 218
Kzo9I GATC 1 cut(s) 249
LpnPI CCDG 5 cut(s) 16, 81, 300, 327, 378
Lsp1109I GCAGC 2 cut(s) 58, 408
LweI GCATC 2 cut(s) 295, 413
MaeI CTAG 2 cut(s) 464, 472
MaeIII GTNAC 2 cut(s) 98, 376
MalI GATC 1 cut(s) 251
MboI GATC 1 cut(s) 249
MboII GAAGA 3 cut(s) 153, 341, 381
MflI RGATCY 1 cut(s) 249
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 58, 241
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 304
MnlI CCTC 1 cut(s) 138
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 216
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 182, 347
MslI CAYNNNNRTG 1 cut(s) 219
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 292
NdeII GATC 1 cut(s) 249
NlaIII CATG 2 cut(s) 106, 218
NlaIV GGNNCC 1 cut(s) 251
NmuCI GTSAC 1 cut(s) 376
NsiI ATGCAT 1 cut(s) 216
NspI RCATGY 1 cut(s) 106
PciI ACATGT 1 cut(s) 102
PctI GAATGC 1 cut(s) 216
PfeI GAWTC 1 cut(s) 367
PkrI GCNGC 2 cut(s) 73, 423
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PscI ACATGT 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 251
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 1 cut(s) 249
PvuII CAGCTG 1 cut(s) 71
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 2 cut(s) 182, 347
SatI GCNGC 2 cut(s) 72, 422
Sau3AI GATC 1 cut(s) 249
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 1 cut(s) 315
SetI ASST 7 cut(s) 73, 93, 148, 297, 302, 449, 454
SfaNI GCATC 2 cut(s) 295, 413
SfcI CTRYAG 1 cut(s) 301
SmiMI CAYNNNNRTG 1 cut(s) 219
Sse9I AATT 2 cut(s) 58, 241
SsiI CCGC 1 cut(s) 424
SspMI CTAG 2 cut(s) 464, 472
StyD4I CCNGG 1 cut(s) 313
TaaI ACNGT 1 cut(s) 115
TaqI TCGA 2 cut(s) 18, 327
TasI AATT 2 cut(s) 58, 241
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 2 cut(s) 182, 347
Tru9I TTAA 2 cut(s) 182, 347
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 2 cut(s) 71, 421
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 1 cut(s) 366
TspRI CASTG 1 cut(s) 385
XapI RAATTY 2 cut(s) 58, 241
XceI RCATGY 1 cut(s) 106
XspI CTAG 2 cut(s) 464, 472
Zsp2I ATGCAT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.