Rw2G033560

Diphthamide synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002016
Physical Location & Seq
Forward (+)
54902938 .. 54905071
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033560.1

Sequence Viewer

Length: 480 bp
ATGGAATCTGGAAATAAAGAGTCTCAAAAGAAAAGTTCACTGGAAGAGTTTGATTCTGAGATTATTGCTGTGGAAAATTTGATGCTAGCTGATGATTCGGTGGACGAGCTTGATAGTTACATGTATCAAACTGTTGGGCACCAGATAGTTATCAGCTATGCAGAATGCATGGGAGTGCCATTGTTCAGAAGGCAAATACAAGGATCCACAAGGCATCAGAAGCTTAGCTATAGAATGACTCCTGGCGATGAAGTCGAAGATATGTTTATTTTGTTAAATGAAGTGAAACGACAGATTCCTTCTGTCACTGCTGTATCTTCTGGTGCTATTGCATCAGACTACCAAAGGTTGTGGGTGGAAAGTGTGTGTTCAAGGTTGGGTCTTGTTTCTGTAGCATACTTGTGGAAACAAGATCAGTCATTGCTCCTTCAAGAAATGATAACTAGTGGGATTGTGGCGAACAGTGAAGCTTCTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Diphthami_syn_2 Pkinase Ribonuc_L-PSP

Protein Analysis

159

Amino Acids

17.83

Weight (kDa)

4.58

Isoelectric Point (pI)

58.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthami_syn_2 PF01902 35 - 150 6.4e-15 Diphthamide synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 138
AclWI GGATC 2 cut(s) 198, 211
AcsI RAATTY 1 cut(s) 76
AflIII ACRYGT 1 cut(s) 120
AgsI TTSAA 2 cut(s) 372, 431
AhlI ACTAGT 1 cut(s) 443
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 6 cut(s) 89, 109, 156, 223, 228, 470
AluI AGCT 6 cut(s) 89, 109, 156, 223, 228, 470
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 2 cut(s) 198, 211
ApoI RAATTY 1 cut(s) 76
AsuNHI GCTAGC 1 cut(s) 85
BaeGI GKGCMC 1 cut(s) 141
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BamHI GGATCC 1 cut(s) 203
BanI GGYRCC 1 cut(s) 138
BciT130I CCWGG 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 27
BcuI ACTAGT 1 cut(s) 443
BfaI CTAG 2 cut(s) 86, 444
BfmI CTRYAG 2 cut(s) 229, 390
BlpI GCTNAGC 1 cut(s) 224
Bme1390I CCNGG 1 cut(s) 243
BmiI GGNNCC 2 cut(s) 140, 205
BmrFI CCNGG 1 cut(s) 243
BmsI GCATC 3 cut(s) 72, 223, 341
BmtI GCTAGC 1 cut(s) 89
Bpu1102I GCTNAGC 1 cut(s) 224
BsaBI GATNNNNATC 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 419
Bse8I GATNNNNATC 1 cut(s) 149
BseBI CCWGG 1 cut(s) 243
BseJI GATNNNNATC 1 cut(s) 149
BseMI GCAATG 1 cut(s) 419
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 45
BseSI GKGCMC 1 cut(s) 141
BshNI GGYRCC 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 2 cut(s) 203, 412
Bsp1720I GCTNAGC 1 cut(s) 224
BspCNI CTCAG 1 cut(s) 49
BspLI GGNNCC 2 cut(s) 140, 205
BspOI GCTAGC 1 cut(s) 89
BspPI GGATC 2 cut(s) 198, 211
BspT107I GGYRCC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 419
BsrI ACTGG 1 cut(s) 45
BssMI GATC 2 cut(s) 203, 412
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 2 cut(s) 133, 464
Bst6I CTCTTC 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 2 cut(s) 57, 224
BstKTI GATC 2 cut(s) 206, 415
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 2 cut(s) 203, 412
BstMWI GCNNNNNNNGC 1 cut(s) 220
BstNI CCWGG 1 cut(s) 243
BstNSI RCATGY 1 cut(s) 124
BstSCI CCNGG 1 cut(s) 241
BstSFI CTRYAG 2 cut(s) 229, 390
BstSLI GKGCMC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 203
BstYI RGATCY 1 cut(s) 203
BtgZI GCGATG 1 cut(s) 261
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 3 cut(s) 38, 306, 469
Cac8I GCNNGC 1 cut(s) 87
CspCI CAANNNNNGTGG 2 cut(s) 332, 367
CviAII CATG 2 cut(s) 121, 169
CviJI RGCY 6 cut(s) 89, 109, 156, 223, 228, 470
CviKI_1 RGCY 6 cut(s) 89, 109, 156, 223, 228, 470
DdeI CTNAG 2 cut(s) 57, 224
DpnI GATC 2 cut(s) 205, 414
DpnII GATC 2 cut(s) 203, 412
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EcoRII CCWGG 1 cut(s) 241
EcoT22I ATGCAT 1 cut(s) 170
FaeI CATG 2 cut(s) 124, 172
FaiI YATR 6 cut(s) 122, 159, 170, 231, 263, 397
FatI CATG 2 cut(s) 120, 168
FspBI CTAG 2 cut(s) 86, 444
Hin1II CATG 2 cut(s) 124, 172
HindIII AAGCTT 2 cut(s) 221, 468
HinfI GANTC 6 cut(s) 5, 20, 53, 95, 238, 295
Hpy166II GTNNAC 2 cut(s) 38, 103
Hpy188I TCNGA 4 cut(s) 58, 188, 219, 337
Hpy188III TCNNGA 2 cut(s) 9, 431
Hpy8I GTNNAC 2 cut(s) 38, 103
HpyAV CCTTC 3 cut(s) 183, 309, 437
HpyCH4III ACNGT 2 cut(s) 133, 464
HpyCH4V TGCA 3 cut(s) 161, 168, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 220
HpyF3I CTNAG 2 cut(s) 57, 224
Hsp92II CATG 2 cut(s) 124, 172
Kzo9I GATC 2 cut(s) 203, 412
LmnI GCTCC 1 cut(s) 429
LpnPI CCDG 5 cut(s) 26, 155, 228, 255, 306
LweI GCATC 3 cut(s) 72, 223, 341
MaeI CTAG 2 cut(s) 86, 444
MaeIII GTNAC 2 cut(s) 116, 304
MalI GATC 2 cut(s) 205, 414
MboI GATC 2 cut(s) 203, 412
MboII GAAGA 3 cut(s) 56, 269, 309
MflI RGATCY 1 cut(s) 203
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 1 cut(s) 76
MlyI GAGTC 2 cut(s) 29, 232
Mph1103I ATGCAT 1 cut(s) 170
MseI TTAA 1 cut(s) 275
MslI CAYNNNNRTG 2 cut(s) 173, 400
MspR9I CCNGG 1 cut(s) 243
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 220
NdeII GATC 2 cut(s) 203, 412
NheI GCTAGC 1 cut(s) 85
NlaIII CATG 2 cut(s) 124, 172
NlaIV GGNNCC 2 cut(s) 140, 205
NmuCI GTSAC 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 170
NspI RCATGY 1 cut(s) 124
PciI ACATGT 1 cut(s) 120
PctI GAATGC 1 cut(s) 170
PfeI GAWTC 4 cut(s) 5, 53, 95, 295
PleI GAGTC 2 cut(s) 28, 232
PpsI GAGTC 2 cut(s) 28, 232
PscI ACATGT 1 cut(s) 120
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 140, 205
PsuI RGATCY 1 cut(s) 203
RseI CAYNNNNRTG 2 cut(s) 173, 400
SaqAI TTAA 1 cut(s) 275
Sau3AI GATC 2 cut(s) 203, 412
SchI GAGTC 2 cut(s) 29, 232
ScrFI CCNGG 1 cut(s) 243
SduI GDGCHC 1 cut(s) 141
SetI ASST 8 cut(s) 91, 111, 158, 225, 230, 350, 377, 472
SfaNI GCATC 3 cut(s) 72, 223, 341
SfcI CTRYAG 2 cut(s) 229, 390
SmiMI CAYNNNNRTG 2 cut(s) 173, 400
SpeI ACTAGT 1 cut(s) 443
Sse9I AATT 1 cut(s) 76
SspMI CTAG 2 cut(s) 86, 444
StyD4I CCNGG 1 cut(s) 241
TaaI ACNGT 2 cut(s) 133, 464
TaqI TCGA 1 cut(s) 255
TasI AATT 1 cut(s) 76
TfiI GAWTC 4 cut(s) 5, 53, 95, 295
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TscAI CASTG 3 cut(s) 45, 313, 469
TseFI GTSAC 1 cut(s) 304
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 264, 294
TspRI CASTG 3 cut(s) 45, 313, 469
XapI RAATTY 1 cut(s) 76
XceI RCATGY 1 cut(s) 124
XspI CTAG 2 cut(s) 86, 444
Zsp2I ATGCAT 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.