Rorug02G0622400

Diphthamide synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002016
Physical Location & Seq
Forward (+)
73888582 .. 73890281
1700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0622400.1

Sequence Viewer

Length: 1392 bp
ATGGACAAGTTGCTTTGTGATGAACTTGTACAAGAAATCTTCTTGAGACTGCCACCACCTTCTTCCTCCTCTGTTTCTCTGGTCTCCAAGCGCTGGCTTCACCTCCACCGCACCTCCAGAACCTCTCTCACTCTCCGCCTCACCGGAGACGATTCCACAGTCGCTTCTCTCTCCTCCCTCCTCTCTCACTACCCTTTCCTCTCTTGCCTCTCTATTCTGTTATCTGATCCCAGAACCAAGACCACTTTGGCTGATCAGCTCCTTCTGTTGGTCTGTACCTTTTGTGCTCAGCTACACAGCCTCAGGTTCTTGGCTGGTCCAGTTTCTGTGTCCTCCCTCAATTCTCTCTCTACAGCATGTACCCAACTCACCTCTCTATGTATCAGTCTCTCCAGGCCTCTCTTCCTCATGTGGATCATCAGTTTTCCAAAATTGAAAGAGTTGTCAGTCATGGTCTGCTCTGGCTTTGGGGTTCAACAAGGAAATGATTATGGTGAGGATTCTGATGCTGAATTGGGTTTAGAGAGTCTTTGTTTATCTGGGATTGGAGCTGGTGATTGGGGATTGGGTTGGCTTTGGAGGAGCTGTAGAAAGCTGAAGAAATTGCAGCTGAAGAGCTGTGAGGGAGTTGGGGATGGGAACTCTTTTTCGTCATTTATTATGTGCTTGCAGGGTGTTCAAGAAGTGGAGCTCAGGACTTGTAGGAGTATACTCGATGGGGTTCTGTTGAAATTGGCCGAAAATTGTGATTCTCTGACTTCTCTTTTGGTTTATGATGGTGGTAGCAGGGAGGGTCTGCTCAGGTTCTTTAGCCAATGCCATTGCAATTTGCAAAAACTCGATTTTCGATTGCCTCTTGACCTTAAGAATGATCATCTATTGGCTGTTGCTACGAAATACAGAAGCCTTTCAAGTATCAGGCTTCAGAGTTGTTGTCTTGTCAGTGGTGAAGGCCTAAAGGCACTCGGGACTGCTGTGAGTTCTGGCCTCGAAGAATTGGCACTAATAAACTGTGATGTGGTTGAAAGGGAACCAGGGTTGCTGTCCACATTAGGACAGAATTTGAGGAAACTGAGGAAATTGGATTTGTCGTACAATGAATTGTTGCTTGACAAGGAATTCATGTCGATGCTAGTTTCGTGTAACTATTTGGTTGATTTGAGGGTGAGAGGGTGCAAAAGGCTTACTAATGCATCCATCATTTCTATGTTAAAGAGCTGTAAATGTTTGGAGAGTGTTGATATTATGCACTGTGGTGGCATTCAGGCTGAAGCAGTCGAGTCTTTCGTTCTGAACTCTCCTCAGCTGAAACGGGTACAGGTGGAACAAAGCAAGATATCAGATGTTGCAAAGACATGGGCCTCGCATAAGTTCATTGAAGCTGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Diphthami_syn_2 Pkinase Ribonuc_L-PSP

Protein Analysis

463

Amino Acids

50.95

Weight (kDa)

7.72

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 93
AccI GTMKAC 1 cut(s) 709
AciI CCGC 2 cut(s) 109, 136
AclWI GGATC 2 cut(s) 221, 422
AcoI YGGCCR 1 cut(s) 735
AcsI RAATTY 2 cut(s) 1060, 1118
AcuI CTGAAG 4 cut(s) 617, 632, 908, 1290
AfaI GTAC 5 cut(s) 30, 277, 361, 1094, 1317
AfeI AGCGCT 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 93, 268
AflII CTTAAG 1 cut(s) 863
AgsI TTSAA 7 cut(s) 436, 476, 680, 730, 912, 1025, 1379
AjnI CCWGG 2 cut(s) 392, 1033
AleI CACNNNNGTG 1 cut(s) 1254
Alw21I GWGCWC 2 cut(s) 289, 693
Alw26I GTCTC 4 cut(s) 40, 88, 141, 392
AlwI GGATC 2 cut(s) 221, 422
AlwNI CAGNNNCTG 1 cut(s) 326
Ama87I CYCGRG 1 cut(s) 965
Aor51HI AGCGCT 1 cut(s) 92
AoxI GGCC 5 cut(s) 395, 735, 952, 985, 1359
ApeKI GCWGC 1 cut(s) 607
ApoI RAATTY 2 cut(s) 1060, 1118
ArsI GACNNNNNNTTYG 2 cut(s) 748, 780
Asp700I GAANNNNTTC 1 cut(s) 907
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 2 cut(s) 317, 1359
AsuHPI GGTGA 7 cut(s) 92, 133, 361, 506, 566, 959, 1177
AvaI CYCGRG 1 cut(s) 965
AvaII GGWCC 1 cut(s) 317
AxyI CCTNAGG 1 cut(s) 302
BanII GRGCYC 1 cut(s) 693
Bbv12I GWGCWC 2 cut(s) 289, 693
BbvCI CCTCAGC 1 cut(s) 1302
BbvI GCAGC 1 cut(s) 619
BccI CCATC 4 cut(s) 629, 710, 770, 1205
BciT130I CCWGG 2 cut(s) 394, 1035
BclI TGATCA 2 cut(s) 253, 871
BcoDI GTCTC 4 cut(s) 40, 88, 141, 392
BfaI CTAG 1 cut(s) 1133
BfmI CTRYAG 2 cut(s) 351, 586
BfoI RGCGCY 1 cut(s) 94
BfrI CTTAAG 1 cut(s) 863
BisI GCNGC 1 cut(s) 608
BlpI GCTNAGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 609
Bme1390I CCNGG 2 cut(s) 394, 1035
Bme18I GGWCC 1 cut(s) 317
BmeT110I CYCGRG 1 cut(s) 965
BmgT120I GGNCC 2 cut(s) 317, 1359
BmiI GGNNCC 1 cut(s) 1032
BmrFI CCNGG 2 cut(s) 394, 1035
BmsI GCATC 3 cut(s) 496, 1119, 1202
BpmI CTGGAG 2 cut(s) 100, 376
Bpu10I CCTNAGC 3 cut(s) 692, 800, 1302
Bpu1102I GCTNAGC 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 64
BsaI GGTCTC 1 cut(s) 88
BsaJI CCNNGG 1 cut(s) 1034
BsaWI WCCGGW 1 cut(s) 143
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 571, 601
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 268
Bse1I ACTGG 1 cut(s) 320
Bse21I CCTNAGG 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 820
BseBI CCWGG 2 cut(s) 394, 1035
BseDI CCNNGG 1 cut(s) 1034
BseGI GGATG 2 cut(s) 640, 1193
BseLI CCNNNNNNNGG 2 cut(s) 93, 268
BseMI GCAATG 1 cut(s) 820
BseMII CTCAG 6 cut(s) 302, 316, 706, 814, 1064, 1316
BseNI ACTGG 1 cut(s) 320
BseRI GAGGAG 5 cut(s) 58, 163, 170, 595, 1290
BseXI GCAGC 1 cut(s) 619
BshFI GGCC 5 cut(s) 397, 737, 954, 987, 1361
BsiHKAI GWGCWC 2 cut(s) 289, 693
BsiHKCI CYCGRG 1 cut(s) 965
BsiSI CCGG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 982
BslI CCNNNNNNNGG 2 cut(s) 93, 268
BsmAI GTCTC 4 cut(s) 40, 88, 141, 392
BsmBI CGTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 982
BsmI GAATGC 1 cut(s) 1260
BsnI GGCC 5 cut(s) 397, 737, 954, 987, 1361
Bso31I GGTCTC 1 cut(s) 88
BsoBI CYCGRG 1 cut(s) 965
Bsp1286I GDGCHC 2 cut(s) 289, 693
Bsp1407I TGTACA 1 cut(s) 28
Bsp143I GATC 4 cut(s) 226, 253, 414, 871
Bsp1720I GCTNAGC 1 cut(s) 288
BspACI CCGC 2 cut(s) 109, 136
BspANI GGCC 5 cut(s) 397, 737, 954, 987, 1361
BspCNI CTCAG 6 cut(s) 301, 315, 705, 813, 1065, 1315
BspLI GGNNCC 1 cut(s) 1032
BspPI GGATC 2 cut(s) 221, 422
BspQI GCTCTTC 1 cut(s) 608
BspTI CTTAAG 1 cut(s) 863
BspTNI GGTCTC 1 cut(s) 88
BsrDI GCAATG 1 cut(s) 820
BsrGI TGTACA 1 cut(s) 28
BsrI ACTGG 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 1034
BssMI GATC 4 cut(s) 226, 253, 414, 871
BssNAI GTATAC 1 cut(s) 710
Bst1107I GTATAC 1 cut(s) 710
Bst2UI CCWGG 2 cut(s) 394, 1035
Bst4CI ACNGT 3 cut(s) 160, 1013, 1253
Bst6I CTCTTC 2 cut(s) 407, 608
BstAFI CTTAAG 1 cut(s) 863
BstAUI TGTACA 1 cut(s) 28
BstC8I GCNNGC 2 cut(s) 95, 668
BstDEI CTNAG 6 cut(s) 288, 302, 692, 800, 1073, 1302
BstF5I GGATG 2 cut(s) 640, 1193
BstH2I RGCGCY 1 cut(s) 94
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 4 cut(s) 229, 256, 417, 874
BstMAI GTCTC 4 cut(s) 40, 88, 141, 392
BstMBI GATC 4 cut(s) 226, 253, 414, 871
BstNI CCWGG 2 cut(s) 394, 1035
BstNSI RCATGY 1 cut(s) 360
BstSCI CCNGG 2 cut(s) 392, 1033
BstSFI CTRYAG 2 cut(s) 351, 586
BstV1I GCAGC 1 cut(s) 619
BstZ17I GTATAC 1 cut(s) 710
Bsu36I CCTNAGG 1 cut(s) 302
BsuRI GGCC 5 cut(s) 397, 737, 954, 987, 1361
BtsCI GGATG 2 cut(s) 640, 1193
BtsIMutI CAGTG 2 cut(s) 949, 1249
Cac8I GCNNGC 2 cut(s) 95, 668
CaiI CAGNNNCTG 1 cut(s) 326
CfoI GCGC 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 317, 1359
Csp6I GTAC 5 cut(s) 29, 276, 360, 1093, 1316
CviAII CATG 5 cut(s) 357, 409, 451, 1123, 1356
CviQI GTAC 5 cut(s) 29, 276, 360, 1093, 1316
DdeI CTNAG 6 cut(s) 288, 302, 692, 800, 1073, 1302
DpnI GATC 4 cut(s) 228, 255, 416, 873
DpnII GATC 4 cut(s) 226, 253, 414, 871
EaeI YGGCCR 1 cut(s) 735
Eam1104I CTCTTC 2 cut(s) 407, 608
EarI CTCTTC 2 cut(s) 407, 608
EciI GGCGGA 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 691
Eco147I AGGCCT 2 cut(s) 397, 954
Eco24I GRGCYC 1 cut(s) 693
Eco31I GGTCTC 1 cut(s) 88
Eco32I GATATC 1 cut(s) 1338
Eco47I GGWCC 1 cut(s) 317
Eco47III AGCGCT 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 691
Eco57I CTGAAG 4 cut(s) 617, 632, 908, 1290
Eco81I CCTNAGG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 965
EcoICRI GAGCTC 1 cut(s) 691
EcoRI GAATTC 1 cut(s) 1118
EcoRII CCWGG 2 cut(s) 392, 1033
EcoRV GATATC 1 cut(s) 1338
EcoT22I ATGCAT 1 cut(s) 1195
EcoT38I GRGCYC 1 cut(s) 693
Esp3I CGTCTC 1 cut(s) 141
FaeI CATG 5 cut(s) 360, 412, 454, 1126, 1359
FaqI GGGAC 1 cut(s) 982
FatI CATG 5 cut(s) 356, 408, 450, 1122, 1355
FbaI TGATCA 2 cut(s) 253, 871
FblI GTMKAC 1 cut(s) 709
Fnu4HI GCNGC 1 cut(s) 608
FokI GGATG 2 cut(s) 647, 1180
FriOI GRGCYC 1 cut(s) 693
Fsp4HI GCNGC 1 cut(s) 608
FspBI CTAG 1 cut(s) 1133
GlaI GCGC 1 cut(s) 92
GluI GCNGC 1 cut(s) 608
GsuI CTGGAG 2 cut(s) 100, 376
HaeII RGCGCY 1 cut(s) 94
HaeIII GGCC 5 cut(s) 397, 737, 954, 987, 1361
HapII CCGG 1 cut(s) 144
HhaI GCGC 1 cut(s) 93
Hin1II CATG 5 cut(s) 360, 412, 454, 1126, 1359
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HinfI GANTC 5 cut(s) 152, 500, 526, 749, 1280
HpaII CCGG 1 cut(s) 144
HphI GGTGA 7 cut(s) 92, 133, 361, 506, 566, 959, 1177
Hpy166II GTNNAC 2 cut(s) 710, 1047
Hpy188I TCNGA 6 cut(s) 226, 505, 756, 927, 1293, 1342
Hpy188III TCNNGA 6 cut(s) 43, 117, 680, 694, 857, 967
Hpy8I GTNNAC 2 cut(s) 710, 1047
HpyAV CCTTC 3 cut(s) 69, 272, 944
HpyCH4III ACNGT 3 cut(s) 160, 1013, 1253
HpyCH4V TGCA 8 cut(s) 607, 670, 825, 832, 1176, 1193, 1249, 1349
HpyF3I CTNAG 6 cut(s) 288, 302, 692, 800, 1073, 1302
Hsp92II CATG 5 cut(s) 360, 412, 454, 1126, 1359
HspAI GCGC 1 cut(s) 91
Ksp22I TGATCA 2 cut(s) 253, 871
Kzo9I GATC 4 cut(s) 226, 253, 414, 871
LguI GCTCTTC 1 cut(s) 608
LmnI GCTCC 4 cut(s) 264, 548, 582, 688
Lsp1109I GCAGC 1 cut(s) 619
LweI GCATC 3 cut(s) 496, 1119, 1202
MaeI CTAG 1 cut(s) 1133
MaeIII GTNAC 1 cut(s) 1142
MalI GATC 4 cut(s) 228, 255, 416, 873
MboI GATC 4 cut(s) 226, 253, 414, 871
MboII GAAGA 6 cut(s) 31, 54, 394, 610, 625, 1004
MhlI GDGCHC 2 cut(s) 289, 693
MlyI GAGTC 2 cut(s) 535, 1289
Mph1103I ATGCAT 1 cut(s) 1195
MroXI GAANNNNTTC 1 cut(s) 907
MseI TTAA 2 cut(s) 864, 1211
MslI CAYNNNNRTG 3 cut(s) 1127, 1205, 1254
MspA1I CMGCKG 2 cut(s) 610, 1306
MspCI CTTAAG 1 cut(s) 863
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 2 cut(s) 394, 1035
Mva1269I GAATGC 1 cut(s) 1260
MvaI CCWGG 2 cut(s) 394, 1035
NdeII GATC 4 cut(s) 226, 253, 414, 871
NlaIII CATG 5 cut(s) 360, 412, 454, 1126, 1359
NlaIV GGNNCC 1 cut(s) 1032
NsiI ATGCAT 1 cut(s) 1195
NspI RCATGY 1 cut(s) 360
OliI CACNNNNGTG 1 cut(s) 1254
PceI AGGCCT 2 cut(s) 397, 954
PciSI GCTCTTC 1 cut(s) 608
PcsI WCGNNNNNNNCGW 1 cut(s) 1284
PctI GAATGC 1 cut(s) 1260
PdmI GAANNNNTTC 1 cut(s) 907
PfeI GAWTC 3 cut(s) 152, 500, 749
PflMI CCANNNNNTGG 1 cut(s) 93
PkrI GCNGC 1 cut(s) 609
PleI GAGTC 2 cut(s) 534, 1288
PpsI GAGTC 2 cut(s) 534, 1288
Psp124BI GAGCTC 1 cut(s) 693
Psp6I CCWGG 2 cut(s) 392, 1033
PspGI CCWGG 2 cut(s) 392, 1033
PspN4I GGNNCC 1 cut(s) 1032
PspPI GGNCC 2 cut(s) 317, 1359
PstNI CAGNNNCTG 1 cut(s) 326
PvuII CAGCTG 2 cut(s) 610, 1306
RsaI GTAC 5 cut(s) 30, 277, 361, 1094, 1317
RsaNI GTAC 5 cut(s) 29, 276, 360, 1093, 1316
RseI CAYNNNNRTG 3 cut(s) 1127, 1205, 1254
SacI GAGCTC 1 cut(s) 693
SapI GCTCTTC 1 cut(s) 608
SaqAI TTAA 2 cut(s) 864, 1211
SatI GCNGC 1 cut(s) 608
Sau3AI GATC 4 cut(s) 226, 253, 414, 871
Sau96I GGNCC 2 cut(s) 317, 1359
SchI GAGTC 2 cut(s) 535, 1289
ScrFI CCNGG 2 cut(s) 394, 1035
SduI GDGCHC 2 cut(s) 289, 693
SfaNI GCATC 3 cut(s) 496, 1119, 1202
SfcI CTRYAG 2 cut(s) 351, 586
SinI GGWCC 1 cut(s) 317
SmiMI CAYNNNNRTG 3 cut(s) 1127, 1205, 1254
SmlI CTYRAG 2 cut(s) 43, 863
SmoI CTYRAG 2 cut(s) 43, 863
SseBI AGGCCT 2 cut(s) 397, 954
SsiI CCGC 2 cut(s) 109, 136
SspMI CTAG 1 cut(s) 1133
SstI GAGCTC 1 cut(s) 693
StuI AGGCCT 2 cut(s) 397, 954
StyD4I CCNGG 2 cut(s) 392, 1033
TaaI ACNGT 3 cut(s) 160, 1013, 1253
TaqI TCGA 6 cut(s) 714, 840, 847, 990, 1127, 1278
TatI WGTACW 1 cut(s) 28
TfiI GAWTC 3 cut(s) 152, 500, 749
Tru1I TTAA 2 cut(s) 864, 1211
Tru9I TTAA 2 cut(s) 864, 1211
TscAI CASTG 2 cut(s) 949, 1256
TseI GCWGC 1 cut(s) 607
TspDTI ATGAA 4 cut(s) 36, 1111, 1113, 1363
TspRI CASTG 2 cut(s) 949, 1256
Van91I CCANNNNNTGG 1 cut(s) 93
Vha464I CTTAAG 1 cut(s) 863
VpaK11BI GGWCC 1 cut(s) 317
XapI RAATTY 2 cut(s) 1060, 1118
XceI RCATGY 1 cut(s) 360
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 709
XmnI GAANNNNTTC 1 cut(s) 907
XspI CTAG 1 cut(s) 1133
Zsp2I ATGCAT 1 cut(s) 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.