Rh2AG659700

Diphthamide synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002016
Physical Location & Seq
Reverse (-)
87558179 .. 87564257
6079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG659700.1

Sequence Viewer

Length: 645 bp
ATGGCCACTGGGCAGATCTCACTTTCCTCACCAAACAGTAATCCCAAAACCAGAGGCCTCTTATTCTTCCTCCCTCTATTGCCGCAACATTTCCAGGCGTCGGAACTTCACCGAGATTTCGGAGAAGGCTTCAGAATGATGGCTAGTGTCCAGGCTGCTGTTTATGGGAGTGAGAATTGTGTGCTTGACCCAACCCTATTTCTTGAAGTTGTAGACTACAAGATTGTTGCTGTGGCAAATTTGACGCCAGCTGATGATTCGGTGGACGAGCTTGATAGTTACATGTATCAAACTATAAGTCTCCTTCCTCTTGTCTATATTTCACCTTATTTGAATGTTTCTTCTTTCAAAAGCAAAGAGTTAAGTTCTGTATGTTGGGCACCAGATAGTTATCAGCTATGCAGATTGCATGGGAGTGCCATTGTTCAGAAGGCGAATACAAGGATCCACAAGGCAAGCCTTCTGAGAAGGGTATTTCGGCATCAAAAGCTTAGCTATAGAATGACTCCTGGTGATGAAGTCGAAGATATGTTTATTTTGTTAAATGAAGTGAAACGACAGATTCCTTCTGTCACTGCTGTATCTTCTGGTGCTATTGCATCAGACTACCAAAGGTTGCGGGTGGAAAGTGTCTGTTCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Diphthami_syn_2 Pkinase Ribonuc_L-PSP

Protein Analysis

214

Amino Acids

23.97

Weight (kDa)

6.95

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 379
AccI GTMKAC 1 cut(s) 213
AciI CCGC 2 cut(s) 83, 619
AclWI GGATC 2 cut(s) 439, 452
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 238
AcuI CTGAAG 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 98, 245
AfiI CCNNNNNNNGG 1 cut(s) 100
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 4 cut(s) 206, 334, 349, 639
AjnI CCWGG 3 cut(s) 93, 150, 508
AluBI AGCT 5 cut(s) 251, 271, 397, 490, 495
AluI AGCT 5 cut(s) 251, 271, 397, 490, 495
Alw26I GTCTC 1 cut(s) 305
AlwI GGATC 2 cut(s) 439, 452
AoxI GGCC 2 cut(s) 3, 55
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 1 cut(s) 238
ArsI GACNNNNNNTTYG 2 cut(s) 283, 315
AsuHPI GGTGA 4 cut(s) 21, 101, 315, 524
BaeGI GKGCMC 1 cut(s) 382
BaeI ACNNNNGTAYC 2 cut(s) 564, 597
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 444
BanI GGYRCC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 142
BccI CCATC 1 cut(s) 133
BciT130I CCWGG 3 cut(s) 95, 152, 510
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 496
BglII AGATCT 1 cut(s) 15
BisI GCNGC 2 cut(s) 83, 156
BlpI GCTNAGC 1 cut(s) 491
BlsI GCNGC 2 cut(s) 84, 157
Bme1390I CCNGG 3 cut(s) 95, 152, 510
BmiI GGNNCC 2 cut(s) 381, 446
BmrFI CCNGG 3 cut(s) 95, 152, 510
BmrI ACTGGG 1 cut(s) 18
BmsI GCATC 2 cut(s) 490, 608
BmuI ACTGGG 1 cut(s) 18
Bpu1102I GCTNAGC 1 cut(s) 491
BsaBI GATNNNNATC 1 cut(s) 390
BsaHI GRCGYC 2 cut(s) 98, 245
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse1I ACTGG 1 cut(s) 13
Bse8I GATNNNNATC 1 cut(s) 390
BseBI CCWGG 3 cut(s) 95, 152, 510
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMII CTCAG 1 cut(s) 455
BseNI ACTGG 1 cut(s) 13
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 1 cut(s) 142
BshFI GGCC 2 cut(s) 5, 57
BshNI GGYRCC 1 cut(s) 379
BslI CCNNNNNNNGG 1 cut(s) 100
BsmAI GTCTC 1 cut(s) 305
BsnI GGCC 2 cut(s) 5, 57
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 2 cut(s) 15, 444
Bsp1720I GCTNAGC 1 cut(s) 491
BspACI CCGC 2 cut(s) 83, 619
BspANI GGCC 2 cut(s) 5, 57
BspCNI CTCAG 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 381, 446
BspPI GGATC 2 cut(s) 439, 452
BspT107I GGYRCC 1 cut(s) 379
BsrI ACTGG 1 cut(s) 13
BssMI GATC 2 cut(s) 15, 444
BssNI GRCGYC 2 cut(s) 98, 245
Bst2UI CCWGG 3 cut(s) 95, 152, 510
Bst4CI ACNGT 1 cut(s) 38
BstACI GRCGYC 2 cut(s) 98, 245
BstC8I GCNNGC 2 cut(s) 249, 457
BstDEI CTNAG 2 cut(s) 464, 491
BstKTI GATC 2 cut(s) 18, 447
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 2 cut(s) 15, 444
BstMWI GCNNNNNNNGC 1 cut(s) 487
BstNI CCWGG 3 cut(s) 95, 152, 510
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 3 cut(s) 93, 150, 508
BstSFI CTRYAG 1 cut(s) 496
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 1 cut(s) 142
BstX2I RGATCY 2 cut(s) 15, 444
BstYI RGATCY 2 cut(s) 15, 444
BsuRI GGCC 2 cut(s) 5, 57
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 2 cut(s) 6, 573
Cac8I GCNNGC 2 cut(s) 249, 457
CseI GACGC 2 cut(s) 87, 253
CviAII CATG 2 cut(s) 283, 410
DdeI CTNAG 2 cut(s) 464, 491
DpnI GATC 2 cut(s) 17, 446
DpnII GATC 2 cut(s) 15, 444
EaeI YGGCCR 1 cut(s) 3
Eco147I AGGCCT 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 115
EcoRII CCWGG 3 cut(s) 93, 150, 508
FaeI CATG 2 cut(s) 286, 413
FaiI YATR 9 cut(s) 165, 284, 296, 318, 373, 400, 411, 498, 530
FatI CATG 2 cut(s) 282, 409
FauI CCCGC 1 cut(s) 612
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 2 cut(s) 83, 156
Fsp4HI GCNGC 2 cut(s) 83, 156
FspBI CTAG 1 cut(s) 144
GluI GCNGC 2 cut(s) 83, 156
HaeIII GGCC 2 cut(s) 5, 57
HgaI GACGC 2 cut(s) 87, 253
Hin1I GRCGYC 2 cut(s) 98, 245
Hin1II CATG 2 cut(s) 286, 413
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 3 cut(s) 257, 505, 562
HphI GGTGA 4 cut(s) 21, 101, 315, 524
Hpy166II GTNNAC 2 cut(s) 214, 265
Hpy188I TCNGA 6 cut(s) 103, 122, 134, 429, 465, 604
Hpy188III TCNNGA 2 cut(s) 203, 639
Hpy8I GTNNAC 2 cut(s) 214, 265
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 6 cut(s) 119, 314, 424, 462, 470, 576
HpyCH4III ACNGT 1 cut(s) 38
HpyCH4V TGCA 3 cut(s) 402, 409, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 487
HpyF3I CTNAG 2 cut(s) 464, 491
Hsp92I GRCGYC 2 cut(s) 98, 245
Hsp92II CATG 2 cut(s) 286, 413
Kzo9I GATC 2 cut(s) 15, 444
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 2 cut(s) 490, 608
MaeI CTAG 1 cut(s) 144
MaeIII GTNAC 2 cut(s) 278, 571
MalI GATC 2 cut(s) 17, 446
MboI GATC 2 cut(s) 15, 444
MboII GAAGA 4 cut(s) 58, 333, 536, 576
MflI RGATCY 2 cut(s) 15, 444
MhlI GDGCHC 1 cut(s) 382
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 175, 238
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 499
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 6 cut(s) 37, 47, 68, 80, 84, 318
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 362, 542
MslI CAYNNNNRTG 1 cut(s) 414
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 251
MspR9I CCNGG 3 cut(s) 95, 152, 510
MvaI CCWGG 3 cut(s) 95, 152, 510
MwoI GCNNNNNNNGC 1 cut(s) 487
NdeII GATC 2 cut(s) 15, 444
NlaIII CATG 2 cut(s) 286, 413
NlaIV GGNNCC 2 cut(s) 381, 446
NmuCI GTSAC 1 cut(s) 571
NspI RCATGY 1 cut(s) 286
PceI AGGCCT 1 cut(s) 57
PciI ACATGT 1 cut(s) 282
PfeI GAWTC 2 cut(s) 257, 562
PkrI GCNGC 2 cut(s) 84, 157
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PscI ACATGT 1 cut(s) 282
Psp6I CCWGG 3 cut(s) 93, 150, 508
PspGI CCWGG 3 cut(s) 93, 150, 508
PspN4I GGNNCC 2 cut(s) 381, 446
PsuI RGATCY 2 cut(s) 15, 444
PvuII CAGCTG 1 cut(s) 251
RseI CAYNNNNRTG 1 cut(s) 414
SaqAI TTAA 2 cut(s) 362, 542
SatI GCNGC 2 cut(s) 83, 156
Sau3AI GATC 2 cut(s) 15, 444
SchI GAGTC 1 cut(s) 499
ScrFI CCNGG 3 cut(s) 95, 152, 510
SduI GDGCHC 1 cut(s) 382
SetI ASST 7 cut(s) 253, 273, 328, 399, 492, 497, 617
SfaNI GCATC 2 cut(s) 490, 608
SfcI CTRYAG 1 cut(s) 496
SmiMI CAYNNNNRTG 1 cut(s) 414
Sse9I AATT 2 cut(s) 175, 238
SseBI AGGCCT 1 cut(s) 57
SsiI CCGC 2 cut(s) 83, 619
SspMI CTAG 1 cut(s) 144
StuI AGGCCT 1 cut(s) 57
StyD4I CCNGG 3 cut(s) 93, 150, 508
TaaI ACNGT 1 cut(s) 38
TaqI TCGA 1 cut(s) 522
TasI AATT 2 cut(s) 175, 238
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 2 cut(s) 257, 562
Tru1I TTAA 2 cut(s) 362, 542
Tru9I TTAA 2 cut(s) 362, 542
TscAI CASTG 2 cut(s) 13, 580
TseFI GTSAC 1 cut(s) 571
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 1 cut(s) 571
TspDTI ATGAA 2 cut(s) 531, 561
TspRI CASTG 2 cut(s) 13, 580
XapI RAATTY 1 cut(s) 238
XceI RCATGY 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.