MD05G1089600.v1.1
MYB Family

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001796
Physical Location & Seq
Forward (+)
18990353 .. 18991237
885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1089600.v1.1.491

Sequence Viewer

Length: 885 bp
ATGGAGTCTTCTGCGATGGTGCAGAGAGAACTGGATCGGGTTAAAGGTCCCTGGAGCCCCGACGAGGATGAGAAGCTACGGCAAATCGTTCAGCGTTACGGCGCTCGCAACTGGTCAGTGATAAGCAAGTCGGTGCCGGGGCGATCGGGCAAGTCCTGCCGGCTGCGGTGGTGCAATCAGCTATCGCCGGAGGTGGAGCACCGGGCGTTCACTCCCGAGGAGGATGAGATCATCGCCGGGGCCCACGCCAAGTACGGAAATAAGTGGGCCACCATCGCCAGACTCCTGAACGGCCGTACGGACAATGCGATCAAGAACCACTGGAACTCGACGTTGAAGCGGAAGTTCTCGGCTCTCTCGCCGAACGAAGACGGCGCGACGATCGACGAAGGTATACTTCGTCCGGAGAAGAAAGCTGCCACGGCGGCGGTGTCGTTTTCTGGTCTCTGTTACAGTCCGGGGAGCCCGTCCGGATCCGACGTCAGCGAGTCTGACGTTCCCGCCAGCTCATCCTCTCAGGTATTCCGGCCCGTGGCAAGAACCGCCGCTATCGTAACTCGAGAGTCGCCTCCGAGTCAGACTGAGTGGTGCGAGCCGTCGACTCTGCTGACTCTGTCATTACCGGGAGCGGAGTCGGCGTTGCACGAGTTGCCAACCAATGAATCGGCCCCTCACCGAGGACAGAGAACGGCGTCGTTGCAGCCGGAGAAGGAGACGAATGTCAATAACAGCAATACGATGTCGTTTGGGCCGGAGTTTATGTCAGTGATGCAGGAGATGATTAGAAAGGAAGTGAGGAGTTACATGGAGGGAGCAGCGTGCTCACAGGGTGAGGGCGTGAGGAATGCTGGGGTTAAGCGCATTGGGATTAGCAGGCTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

295

Amino Acids

32.22

Weight (kDa)

7.66

Isoelectric Point (pI)

55.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 15 - 61 1.8e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 18 - 75 1.4e-16 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 69 - 111 5e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 70 - 120 5.2e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 483
AccB1I GGYRCC 1 cut(s) 133
AccBSI CCGCTC 1 cut(s) 629
AccI GTMKAC 2 cut(s) 394, 599
AccII CGCG 1 cut(s) 377
AccIII TCCGGA 2 cut(s) 403, 470
AciI CCGC 8 cut(s) 166, 340, 425, 428, 501, 543, 546, 629
AclWI GGATC 3 cut(s) 42, 468, 481
AcoI YGGCCR 1 cut(s) 292
AcyI GRCGYC 2 cut(s) 480, 692
AdeI CACNNNGTG 1 cut(s) 830
AfaI GTAC 2 cut(s) 254, 298
AfiI CCNNNNNNNGG 3 cut(s) 64, 532, 677
AgsI TTSAA 1 cut(s) 337
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 4 cut(s) 76, 181, 416, 507
AluI AGCT 4 cut(s) 76, 181, 416, 507
Alw21I GWGCWC 2 cut(s) 201, 824
Alw26I GTCTC 2 cut(s) 449, 707
AlwI GGATC 3 cut(s) 42, 468, 481
Ama87I CYCGRG 2 cut(s) 215, 558
Aor13HI TCCGGA 2 cut(s) 403, 470
AoxI GGCC 6 cut(s) 240, 267, 292, 527, 666, 749
ApaI GGGCCC 1 cut(s) 244
ApeKI GCWGC 4 cut(s) 163, 416, 700, 815
AspLEI GCGC 3 cut(s) 104, 377, 861
AspS9I GGNCC 7 cut(s) 47, 240, 241, 267, 528, 667, 749
AsuC2I CCSGG 5 cut(s) 138, 203, 238, 459, 624
AsuHPI GGTGA 2 cut(s) 665, 842
AvaI CYCGRG 2 cut(s) 215, 558
AvaII GGWCC 1 cut(s) 47
BaeGI GKGCMC 1 cut(s) 244
BamHI GGATCC 1 cut(s) 473
BanI GGYRCC 1 cut(s) 133
BanII GRGCYC 3 cut(s) 59, 244, 467
BauI CACGAG 1 cut(s) 644
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 2 cut(s) 201, 824
BbvI GCAGC 4 cut(s) 150, 403, 712, 827
BccI CCATC 2 cut(s) 10, 281
BceAI ACGGC 8 cut(s) 95, 115, 279, 307, 388, 438, 580, 705
BciT130I CCWGG 1 cut(s) 52
BcnI CCSGG 5 cut(s) 138, 203, 238, 459, 624
BcoDI GTCTC 2 cut(s) 449, 707
BfoI RGCGCY 1 cut(s) 105
BglI GCCNNNNNGGC 1 cut(s) 425
BisI GCNGC 6 cut(s) 164, 417, 426, 546, 701, 816
BlsI GCNGC 6 cut(s) 165, 418, 427, 547, 702, 817
Bme1390I CCNGG 6 cut(s) 52, 138, 203, 238, 459, 624
Bme18I GGWCC 1 cut(s) 47
BmeT110I CYCGRG 2 cut(s) 215, 558
BmgT120I GGNCC 7 cut(s) 47, 240, 241, 267, 528, 667, 749
BmiI GGNNCC 8 cut(s) 49, 56, 135, 241, 242, 464, 475, 669
BmrFI CCNGG 6 cut(s) 52, 138, 203, 238, 459, 624
BmsI GCATC 1 cut(s) 759
BoxI GACNNNNGTC 1 cut(s) 719
BpiI GAAGAC 1 cut(s) 375
BpmI CTGGAG 1 cut(s) 73
BpuMI CCSGG 5 cut(s) 138, 203, 238, 459, 624
BsaHI GRCGYC 2 cut(s) 480, 692
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 8 cut(s) 50, 137, 216, 237, 420, 458, 531, 676
BsaWI WCCGGW 2 cut(s) 403, 470
BsaXI ACNNNNNCTCC 4 cut(s) 623, 653, 746, 776
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 532, 677
Bse118I RCCGGY 1 cut(s) 159
Bse1I ACTGG 3 cut(s) 36, 116, 326
BseAI TCCGGA 2 cut(s) 403, 470
BseBI CCWGG 1 cut(s) 52
BseDI CCNNGG 8 cut(s) 50, 137, 216, 237, 420, 458, 531, 676
BseGI GGATG 3 cut(s) 73, 229, 509
BseLI CCNNNNNNNGG 3 cut(s) 64, 532, 677
BseMII CTCAG 2 cut(s) 530, 573
BseNI ACTGG 3 cut(s) 36, 116, 326
BseRI GAGGAG 2 cut(s) 233, 811
BseSI GKGCMC 1 cut(s) 244
BseX3I CGGCCG 1 cut(s) 292
BseXI GCAGC 4 cut(s) 150, 403, 712, 827
BseYI CCCAGC 1 cut(s) 848
BsgI GTGCAG 1 cut(s) 41
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 3 cut(s) 146, 295, 384
BshFI GGCC 6 cut(s) 242, 269, 294, 529, 668, 751
BshNI GGYRCC 1 cut(s) 133
BsiEI CGRYCG 3 cut(s) 146, 295, 384
BsiHKAI GWGCWC 2 cut(s) 201, 824
BsiHKCI CYCGRG 2 cut(s) 215, 558
BsiWI CGTACG 1 cut(s) 296
BslFI GGGAC 1 cut(s) 33
BslI CCNNNNNNNGG 3 cut(s) 64, 532, 677
BsmAI GTCTC 2 cut(s) 449, 707
BsmBI CGTCTC 1 cut(s) 707
BsmFI GGGAC 1 cut(s) 33
BsmI GAATGC 1 cut(s) 850
BsnI GGCC 6 cut(s) 242, 269, 294, 529, 668, 751
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 2 cut(s) 215, 558
Bsp120I GGGCCC 1 cut(s) 240
Bsp1286I GDGCHC 5 cut(s) 59, 201, 244, 467, 824
Bsp13I TCCGGA 2 cut(s) 403, 470
Bsp143I GATC 6 cut(s) 34, 143, 228, 309, 381, 473
BspACI CCGC 8 cut(s) 166, 340, 425, 428, 501, 543, 546, 629
BspANI GGCC 6 cut(s) 242, 269, 294, 529, 668, 751
BspCNI CTCAG 2 cut(s) 529, 574
BspEI TCCGGA 2 cut(s) 403, 470
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 8 cut(s) 49, 56, 135, 241, 242, 464, 475, 669
BspPI GGATC 3 cut(s) 42, 468, 481
BspT107I GGYRCC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 449
BsrBI CCGCTC 1 cut(s) 629
BsrFI RCCGGY 1 cut(s) 159
BsrI ACTGG 3 cut(s) 36, 116, 326
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 8 cut(s) 50, 137, 216, 237, 420, 458, 531, 676
BssMI GATC 6 cut(s) 34, 143, 228, 309, 381, 473
BssNAI GTATAC 1 cut(s) 395
BssNI GRCGYC 2 cut(s) 480, 692
BssSI CACGAG 1 cut(s) 644
Bst1107I GTATAC 1 cut(s) 395
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 1 cut(s) 455
BstACI GRCGYC 2 cut(s) 480, 692
BstAPI GCANNNNNTGC 2 cut(s) 156, 649
BstC8I GCNNGC 6 cut(s) 106, 161, 505, 593, 820, 875
BstDEI CTNAG 2 cut(s) 516, 582
BstDSI CCRYGG 2 cut(s) 420, 531
BstENI CCTNNNNNAGG 1 cut(s) 675
BstF5I GGATG 3 cut(s) 73, 229, 509
BstFNI CGCG 1 cut(s) 377
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 3 cut(s) 104, 377, 861
BstKTI GATC 6 cut(s) 37, 146, 231, 312, 384, 476
BstMAI GTCTC 2 cut(s) 449, 707
BstMBI GATC 6 cut(s) 34, 143, 228, 309, 381, 473
BstMCI CGRYCG 3 cut(s) 146, 295, 384
BstMWI GCNNNNNNNGC 7 cut(s) 156, 275, 422, 425, 542, 635, 649
BstNI CCWGG 1 cut(s) 52
BstPAI GACNNNNGTC 1 cut(s) 719
BstSCI CCNGG 6 cut(s) 50, 136, 201, 236, 457, 622
BstSLI GKGCMC 1 cut(s) 244
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 4 cut(s) 150, 403, 712, 827
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
BstZ17I GTATAC 1 cut(s) 395
BstZI CGGCCG 1 cut(s) 292
BsuRI GGCC 6 cut(s) 242, 269, 294, 529, 668, 751
BtgI CCRYGG 2 cut(s) 420, 531
BtgZI GCGATG 3 cut(s) 29, 217, 259
BtsCI GGATG 3 cut(s) 73, 229, 509
BtsIMutI CAGTG 3 cut(s) 123, 319, 771
Cac8I GCNNGC 6 cut(s) 106, 161, 505, 593, 820, 875
CfoI GCGC 3 cut(s) 104, 377, 861
Cfr10I RCCGGY 1 cut(s) 159
Cfr13I GGNCC 7 cut(s) 47, 240, 241, 267, 528, 667, 749
CseI GACGC 1 cut(s) 681
Csp6I GTAC 2 cut(s) 253, 297
CviAII CATG 1 cut(s) 805
CviQI GTAC 2 cut(s) 253, 297
DdeI CTNAG 2 cut(s) 516, 582
DpnI GATC 6 cut(s) 36, 145, 230, 311, 383, 475
DpnII GATC 6 cut(s) 34, 143, 228, 309, 381, 473
DraIII CACNNNGTG 1 cut(s) 830
EaeI YGGCCR 1 cut(s) 292
EagI CGGCCG 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 292
Eco24I GRGCYC 3 cut(s) 59, 244, 467
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 1 cut(s) 47
Eco52I CGGCCG 1 cut(s) 292
Eco88I CYCGRG 2 cut(s) 215, 558
EcoNI CCTNNNNNAGG 1 cut(s) 675
EcoO109I RGGNCCY 2 cut(s) 47, 240
EcoRII CCWGG 1 cut(s) 50
EcoT38I GRGCYC 3 cut(s) 59, 244, 467
Esp3I CGTCTC 1 cut(s) 707
FaeI CATG 1 cut(s) 808
FaiI YATR 3 cut(s) 395, 761, 806
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 1 cut(s) 33
FatI CATG 1 cut(s) 804
FauI CCCGC 1 cut(s) 508
FblI GTMKAC 2 cut(s) 394, 599
Fnu4HI GCNGC 6 cut(s) 164, 417, 426, 546, 701, 816
FokI GGATG 3 cut(s) 80, 236, 496
FriOI GRGCYC 3 cut(s) 59, 244, 467
Fsp4HI GCNGC 6 cut(s) 164, 417, 426, 546, 701, 816
GlaI GCGC 3 cut(s) 103, 376, 860
GluI GCNGC 6 cut(s) 164, 417, 426, 546, 701, 816
GsaI CCCAGC 1 cut(s) 852
GsuI CTGGAG 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 6 cut(s) 242, 269, 294, 529, 668, 751
HgaI GACGC 1 cut(s) 681
HhaI GCGC 3 cut(s) 104, 377, 861
Hin1I GRCGYC 2 cut(s) 480, 692
Hin1II CATG 1 cut(s) 808
Hin6I GCGC 3 cut(s) 102, 375, 859
HinP1I GCGC 3 cut(s) 102, 375, 859
HincII GTYRAC 1 cut(s) 600
HindII GTYRAC 1 cut(s) 600
HinfI GANTC 9 cut(s) 5, 282, 488, 563, 574, 601, 610, 632, 662
HphI GGTGA 2 cut(s) 665, 842
Hpy166II GTNNAC 3 cut(s) 210, 395, 600
Hpy188I TCNGA 4 cut(s) 478, 493, 573, 579
Hpy188III TCNNGA 6 cut(s) 215, 286, 313, 404, 471, 560
Hpy8I GTNNAC 3 cut(s) 210, 395, 600
Hpy99I CGWCG 7 cut(s) 65, 334, 382, 389, 482, 601, 697
HpyAV CCTTC 2 cut(s) 383, 703
HpyCH4III ACNGT 1 cut(s) 455
HpyCH4IV ACGT 3 cut(s) 332, 480, 495
HpyCH4V TGCA 5 cut(s) 22, 174, 643, 700, 772
HpyF10VI GCNNNNNNNGC 7 cut(s) 156, 275, 422, 425, 542, 635, 649
HpyF3I CTNAG 2 cut(s) 516, 582
HpySE526I ACGT 3 cut(s) 332, 480, 495
Hsp92I GRCGYC 2 cut(s) 480, 692
Hsp92II CATG 1 cut(s) 808
HspAI GCGC 3 cut(s) 102, 375, 859
Kpn2I TCCGGA 2 cut(s) 403, 470
KroI GCCGGC 1 cut(s) 159
KroNI GCCGGC 1 cut(s) 161
Kzo9I GATC 6 cut(s) 34, 143, 228, 309, 381, 473
LmnI GCTCC 5 cut(s) 54, 196, 462, 626, 812
Lsp1109I GCAGC 4 cut(s) 150, 403, 712, 827
LweI GCATC 1 cut(s) 759
MaeII ACGT 3 cut(s) 332, 480, 495
MaeIII GTNAC 4 cut(s) 95, 449, 553, 800
MalI GATC 6 cut(s) 36, 145, 230, 311, 383, 475
MbiI CCGCTC 1 cut(s) 629
MboI GATC 6 cut(s) 34, 143, 228, 309, 381, 473
MboII GAAGA 2 cut(s) 380, 421
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 5 cut(s) 59, 201, 244, 467, 824
MlyI GAGTC 8 cut(s) 14, 276, 497, 572, 583, 595, 604, 641
MmeI TCCRAC 1 cut(s) 501
MroI TCCGGA 2 cut(s) 403, 470
MroNI GCCGGC 1 cut(s) 159
MseI TTAA 2 cut(s) 42, 855
MspR9I CCNGG 6 cut(s) 52, 138, 203, 238, 459, 624
Mva1269I GAATGC 1 cut(s) 850
MvaI CCWGG 1 cut(s) 52
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 7 cut(s) 156, 275, 422, 425, 542, 635, 649
NaeI GCCGGC 1 cut(s) 161
NciI CCSGG 5 cut(s) 138, 203, 238, 459, 624
NdeII GATC 6 cut(s) 34, 143, 228, 309, 381, 473
NgoMIV GCCGGC 1 cut(s) 159
NlaIII CATG 1 cut(s) 808
NlaIV GGNNCC 8 cut(s) 49, 56, 135, 241, 242, 464, 475, 669
NmeAIII GCCGAG 1 cut(s) 329
PaeR7I CTCGAG 1 cut(s) 558
PcsI WCGNNNNNNNCGW 1 cut(s) 305
PctI GAATGC 1 cut(s) 850
PdiI GCCGGC 1 cut(s) 161
PfeI GAWTC 1 cut(s) 662
Pfl23II CGTACG 1 cut(s) 296
PflFI GACNNNGTC 1 cut(s) 613
PkrI GCNGC 6 cut(s) 165, 418, 427, 547, 702, 817
Ple19I CGATCG 2 cut(s) 146, 384
PleI GAGTC 8 cut(s) 13, 276, 496, 571, 582, 595, 604, 640
PpsI GAGTC 8 cut(s) 13, 276, 496, 571, 582, 595, 604, 640
PpuMI RGGWCCY 1 cut(s) 47
PshAI GACNNNNGTC 1 cut(s) 719
Psp5II RGGWCCY 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 848
PspGI CCWGG 1 cut(s) 50
PspLI CGTACG 1 cut(s) 296
PspN4I GGNNCC 8 cut(s) 49, 56, 135, 241, 242, 464, 475, 669
PspOMI GGGCCC 1 cut(s) 240
PspPI GGNCC 7 cut(s) 47, 240, 241, 267, 528, 667, 749
PspPPI RGGWCCY 1 cut(s) 47
PsuI RGATCY 1 cut(s) 473
PsyI GACNNNGTC 1 cut(s) 613
PvuI CGATCG 2 cut(s) 146, 384
RsaI GTAC 2 cut(s) 254, 298
RsaNI GTAC 2 cut(s) 253, 297
SalI GTCGAC 1 cut(s) 598
SaqAI TTAA 2 cut(s) 42, 855
SatI GCNGC 6 cut(s) 164, 417, 426, 546, 701, 816
Sau3AI GATC 6 cut(s) 34, 143, 228, 309, 381, 473
Sau96I GGNCC 7 cut(s) 47, 240, 241, 267, 528, 667, 749
SchI GAGTC 8 cut(s) 14, 276, 497, 572, 583, 595, 604, 641
ScrFI CCNGG 6 cut(s) 52, 138, 203, 238, 459, 624
SduI GDGCHC 5 cut(s) 59, 201, 244, 467, 824
SfaNI GCATC 1 cut(s) 759
Sfr274I CTCGAG 1 cut(s) 558
SinI GGWCC 1 cut(s) 47
SlaI CTCGAG 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 558
SmoI CTYRAG 1 cut(s) 558
SsiI CCGC 8 cut(s) 166, 340, 425, 428, 501, 543, 546, 629
StyD4I CCNGG 6 cut(s) 50, 136, 201, 236, 457, 622
TaaI ACNGT 1 cut(s) 455
TaiI ACGT 3 cut(s) 335, 483, 498
TaqI TCGA 5 cut(s) 329, 384, 559, 599, 879
TauI GCSGC 2 cut(s) 428, 548
TfiI GAWTC 1 cut(s) 662
Tru1I TTAA 2 cut(s) 42, 855
Tru9I TTAA 2 cut(s) 42, 855
TscAI CASTG 3 cut(s) 123, 326, 771
TseI GCWGC 4 cut(s) 163, 416, 700, 815
TspDTI ATGAA 1 cut(s) 675
TspGWI ACGGA 2 cut(s) 270, 314
TspRI CASTG 3 cut(s) 123, 326, 771
Tth111I GACNNNGTC 1 cut(s) 613
VpaK11BI GGWCC 1 cut(s) 47
XagI CCTNNNNNAGG 1 cut(s) 675
XhoI CTCGAG 1 cut(s) 558
XmiI GTMKAC 2 cut(s) 394, 599
ZraI GACGTC 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.