Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002826

Physical Location & Seq

Genomic Coordinates Reverse (-)

25188408 .. 25189937

Gene Structure 1530 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1128000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1077 bp

ATGGAAGATACTCAAAGAGTGTTAACATGGGAAGAGGAAGAAGAACATGCCAAGGTGGATGTGTGGAAATATGTTTTTGGGTTTGTTGAAATCGCGGTGGTAAAATGTGCCATTGAGCTCGGAATAGCTGAAGCAATTGAAAGCCATGGAAGCCCCATGACACTCTTAGAGCTCTCATCTGCTCTAAGTTGTGATCCTTCTCACCTTTACCGCGTCATGAGGGTACTAGTCCACCTCAAATTATTCAAAGAAATAACCACAAACCAATTAGGCTCCAAAGGTTATGCACAAACACCTCTGTCTCACCGGCTACTGAAATCCGGAGAAAATAGCATGGTGGCGCTGATCTTGCTGGAGAGTAGTCCGGTAATGCTGGCACCATGGCACGGCTTAAGTGCCCGAATTCGAGGAAATATTAGCAATCAATTGTTTGAGGAAGTACATGGGGAGGACTTATGGAGCTTTGGTGCGGCCAATCCTGATCACAGCAAGCTTTTCAATGATGCAATGACTTGTGATGTAAAGGCAGCTGTGCCTGCAGTGATTAAAAGTTGTATAGAGGTGTTCAAAGGCCTTGAGACAATAGTAGATGTCGGCGGGGGAAATGGGACTATGTTGCGCTTGTTGGTCGAGGGTTGCCCTTGGATTCGTGGCATTAACTTTGATCTTCCGCATGTTGTCTCTGCTGCTCAGGATTGTGATCGCGTTGAGAATGTTGGAGGTGACATGTTTGATCGCGTTCCAAAGGCTGATGCAGTAATCATCAAGGGGGTTCTTCATAACTGGGGTGACGATGACTGCATTCGTATCCTTAAAAAGTGCCGGGAAGCCGTTCCTAAGGACACGGGGAAGGTGATCATCATAGATGCCGTTATCGACGAAAAATATGAGAAAGAAGACAAGAAGCTAGCAAATCTGAAATTGATGTTAGATATGGTGATGATTGCCCATACTAACAAAGGCAAAGAAAGGAGCTTGAAGGAATGGGAATATGTTCTTGGGGGGGCTGGCTTTAGTGGACACACTATCACACCTGTTCCTGCTATCCAATATTCTGTTATTCAAGCTTTTCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008757 GO:0009058 GO:0009987 GO:0010817 GO:0016740 GO:0016741 GO:0017096 GO:0017144 GO:0018130 GO:0019438 GO:0030186 GO:0030187 GO:0032259 GO:0034641 GO:0034754 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0065007 GO:0065008 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dimerisation Methyltransf_2

Protein Analysis

359

Amino Acids

39.6

Weight (kDa)

5.5

Isoelectric Point (pI)

26.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dimerisation	PF08100	22 - 107	8.6e-21	O-methyltransferase dimerisation domain
Methyltransf_2	PF00891	128 - 339	8.8e-50	O-methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002826)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35150 AT4G35160
fragaria_vesca	FvH4_2g15840 FvH4_2g15880 FvH4_2g15880
malus_domestica	MD05G1127400.v1.1 MD05G1127600.v1.1 MD05G1127900.v1.1 MD05G1128000.v1.1 MD05G1128100.v1.1
prunus_persica	Prupe.8G171000_v2.0.a1 Prupe.8G171100_v2.0.a1 Prupe.8G171400_v2.0.a1 Prupe.8G171900_v2.0.a1 Prupe.8G172000_v2.0.a1
pyrus_communis	pycom05g12360 pycom05g12370 pycom05g12400
rosa_chinensis	RchiOBHm_Chr6g0279261 RchiOBHm_Chr6g0279331 RchiOBHm_Chr6g0279441
rosa_laevigata	RLG00000013151 RLG00000013152
rosa_multiflora	Rmu_sc0009584.1_g000002 Rmu_sc0026652.1_g000001
rosa_roxburghii	Rroxscaffold_7G00188960 Rroxscaffold_7G00188980 Rroxscaffold_7G00189010
rosa_rugosa	Rorug06G0124200 Rorug06G0124200
rosa_samantha	Rh6AG232600 Rh6AG232800 Rh6AG233200 Rh6BG237200 Rh6BG237500 Rh6CG239300 Rh6CG239700
rosa_wichuraiana	Rw6G020330 Rw6G020360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	376
AccII	CGCG	4 cut(s)	95, 213, 705, 738
AccIII	TCCGGA	1 cut(s)	320
AciI	CCGC	5 cut(s)	95, 211, 470, 597, 671
AclWI	GGATC	1 cut(s)	188
AcoI	YGGCCR	1 cut(s)	471
AcsI	RAATTY	1 cut(s)	402
AcuI	CTGAAG	1 cut(s)	150
AfaI	GTAC	2 cut(s)	225, 441
AfiI	CCNNNNNNNGG	1 cut(s)	386
AflII	CTTAAG	1 cut(s)	391
AflIII	ACRYGT	1 cut(s)	726
AgsI	TTSAA	7 cut(s)	89, 140, 247, 499, 568, 979, 1064
AhlI	ACTAGT	1 cut(s)	226
AjuI	GAANNNNNNNTTGG	2 cut(s)	981, 1013
AluBI	AGCT	9 cut(s)	118, 128, 172, 462, 493, 530, 907, 975, 1067
AluI	AGCT	9 cut(s)	118, 128, 172, 462, 493, 530, 907, 975, 1067
Alw21I	GWGCWC	2 cut(s)	120, 174
Alw26I	GTCTC	3 cut(s)	306, 572, 685
AlwI	GGATC	1 cut(s)	188
Aor13HI	TCCGGA	1 cut(s)	320
AoxI	GGCC	2 cut(s)	471, 571
ApeKI	GCWGC	2 cut(s)	527, 686
ApoI	RAATTY	1 cut(s)	402
Asp700I	GAANNNNTTC	2 cut(s)	831, 993
AspLEI	GCGC	2 cut(s)	343, 621
AsuC2I	CCSGG	1 cut(s)	824
AsuHPI	GGTGA	6 cut(s)	194, 296, 734, 800, 865, 949
AsuNHI	GCTAGC	1 cut(s)	907
AxyI	CCTNAGG	1 cut(s)	837
BaeGI	GKGCMC	1 cut(s)	400
BanI	GGYRCC	1 cut(s)	376
BanII	GRGCYC	2 cut(s)	120, 174
BbsI	GAAGAC	1 cut(s)	903
Bbv12I	GWGCWC	2 cut(s)	120, 174
BbvI	GCAGC	2 cut(s)	539, 673
BceAI	ACGGC	3 cut(s)	403, 815, 854
BciVI	GTATCC	1 cut(s)	818
BclI	TGATCA	2 cut(s)	481, 855
BcnI	CCSGG	1 cut(s)	824
BcoDI	GTCTC	3 cut(s)	306, 572, 685
BcuI	ACTAGT	1 cut(s)	226
BfaI	CTAG	2 cut(s)	227, 908
BfmI	CTRYAG	1 cut(s)	537
BfoI	RGCGCY	1 cut(s)	344
BfrI	CTTAAG	1 cut(s)	391
BfuI	GTATCC	1 cut(s)	818
BisI	GCNGC	3 cut(s)	471, 528, 687
BlsI	GCNGC	3 cut(s)	472, 529, 688
Bme1390I	CCNGG	1 cut(s)	824
BmiI	GGNNCC	2 cut(s)	274, 378
BmrFI	CCNGG	1 cut(s)	824
BmrI	ACTGGG	1 cut(s)	793
BmsI	GCATC	3 cut(s)	493, 742, 856
BmtI	GCTAGC	1 cut(s)	911
BmuI	ACTGGG	1 cut(s)	793
BpiI	GAAGAC	1 cut(s)	903
BpmI	CTGGAG	1 cut(s)	374
Bpu10I	CCTNAGC	1 cut(s)	690
BpuEI	CTTGAG	1 cut(s)	596
BpuMI	CCSGG	1 cut(s)	824
BsaBI	GATNNNNATC	1 cut(s)	699
BsaJI	CCNNGG	4 cut(s)	51, 145, 380, 641
BsaWI	WCCGGW	2 cut(s)	320, 364
BsaXI	ACNNNNNCTCC	2 cut(s)	451, 481
Bsc4I	CCNNNNNNNGG	1 cut(s)	386
Bse118I	RCCGGY	1 cut(s)	306
Bse1I	ACTGG	1 cut(s)	788
Bse21I	CCTNAGG	1 cut(s)	837
Bse3DI	GCAATG	1 cut(s)	513
Bse8I	GATNNNNATC	1 cut(s)	699
BseAI	TCCGGA	1 cut(s)	320
BseDI	CCNNGG	4 cut(s)	51, 145, 380, 641
BseGI	GGATG	1 cut(s)	64
BseJI	GATNNNNATC	1 cut(s)	699
BseLI	CCNNNNNNNGG	1 cut(s)	386
BseMI	GCAATG	1 cut(s)	513
BseMII	CTCAG	1 cut(s)	704
BseNI	ACTGG	1 cut(s)	788
BseSI	GKGCMC	1 cut(s)	400
BseXI	GCAGC	2 cut(s)	539, 673
Bsh1236I	CGCG	4 cut(s)	95, 213, 705, 738
BshFI	GGCC	2 cut(s)	473, 573
BshNI	GGYRCC	1 cut(s)	376
BsiHKAI	GWGCWC	2 cut(s)	120, 174
BsiSI	CCGG	4 cut(s)	307, 321, 365, 823
BslFI	GGGAC	1 cut(s)	622
BslI	CCNNNNNNNGG	1 cut(s)	386
BsmAI	GTCTC	3 cut(s)	306, 572, 685
BsmFI	GGGAC	1 cut(s)	622
BsmI	GAATGC	1 cut(s)	801
BsnI	GGCC	2 cut(s)	473, 573
Bsp1286I	GDGCHC	3 cut(s)	120, 174, 400
Bsp13I	TCCGGA	1 cut(s)	320
Bsp143I	GATC	7 cut(s)	193, 345, 481, 664, 700, 733, 855
Bsp19I	CCATGG	2 cut(s)	145, 380
BspACI	CCGC	5 cut(s)	95, 211, 470, 597, 671
BspANI	GGCC	2 cut(s)	473, 573
BspCNI	CTCAG	1 cut(s)	703
BspEI	TCCGGA	1 cut(s)	320
BspFNI	CGCG	4 cut(s)	95, 213, 705, 738
BspHI	TCATGA	1 cut(s)	216
BspLI	GGNNCC	2 cut(s)	274, 378
BspMAI	CTGCAG	1 cut(s)	541
BspOI	GCTAGC	1 cut(s)	911
BspPI	GGATC	1 cut(s)	188
BspT107I	GGYRCC	1 cut(s)	376
BspTI	CTTAAG	1 cut(s)	391
BsrDI	GCAATG	1 cut(s)	513
BsrFI	RCCGGY	1 cut(s)	306
BsrI	ACTGG	1 cut(s)	788
BssAI	RCCGGY	1 cut(s)	306
BssECI	CCNNGG	4 cut(s)	51, 145, 380, 641
BssMI	GATC	7 cut(s)	193, 345, 481, 664, 700, 733, 855
BssT1I	CCWWGG	4 cut(s)	51, 145, 380, 641
Bst6I	CTCTTC	1 cut(s)	27
BstAFI	CTTAAG	1 cut(s)	391
BstC8I	GCNNGC	5 cut(s)	375, 491, 537, 909, 1009
BstDEI	CTNAG	4 cut(s)	166, 185, 690, 837
BstDSI	CCRYGG	2 cut(s)	145, 380
BstF5I	GGATG	1 cut(s)	64
BstFNI	CGCG	4 cut(s)	95, 213, 705, 738
BstH2I	RGCGCY	1 cut(s)	344
BstHHI	GCGC	2 cut(s)	343, 621
BstKTI	GATC	7 cut(s)	196, 348, 484, 667, 703, 736, 858
BstMAI	GTCTC	3 cut(s)	306, 572, 685
BstMBI	GATC	7 cut(s)	193, 345, 481, 664, 700, 733, 855
BstMWI	GCNNNNNNNGC	3 cut(s)	150, 349, 536
BstNSI	RCATGY	3 cut(s)	50, 677, 730
BstSCI	CCNGG	1 cut(s)	822
BstSFI	CTRYAG	1 cut(s)	537
BstSLI	GKGCMC	1 cut(s)	400
BstUI	CGCG	4 cut(s)	95, 213, 705, 738
BstV1I	GCAGC	2 cut(s)	539, 673
BstV2I	GAAGAC	1 cut(s)	903
Bsu36I	CCTNAGG	1 cut(s)	837
BsuI	GTATCC	1 cut(s)	818
BsuRI	GGCC	2 cut(s)	473, 573
BtgI	CCRYGG	2 cut(s)	145, 380
BtsCI	GGATG	1 cut(s)	64
BtsI	GCAGTG	1 cut(s)	546
BtsIMutI	CAGTG	1 cut(s)	546
Cac8I	GCNNGC	5 cut(s)	375, 491, 537, 909, 1009
CciI	TCATGA	1 cut(s)	216
CfoI	GCGC	2 cut(s)	343, 621
Cfr10I	RCCGGY	1 cut(s)	306
CseI	GACGC	1 cut(s)	202
Csp6I	GTAC	2 cut(s)	224, 440
CviQI	GTAC	2 cut(s)	224, 440
DdeI	CTNAG	4 cut(s)	166, 185, 690, 837
DpnI	GATC	7 cut(s)	195, 347, 483, 666, 702, 735, 857
DpnII	GATC	7 cut(s)	193, 345, 481, 664, 700, 733, 855
EaeI	YGGCCR	1 cut(s)	471
Eam1104I	CTCTTC	1 cut(s)	27
EarI	CTCTTC	1 cut(s)	27
Ecl136II	GAGCTC	2 cut(s)	118, 172
Eco130I	CCWWGG	4 cut(s)	51, 145, 380, 641
Eco147I	AGGCCT	1 cut(s)	573
Eco24I	GRGCYC	2 cut(s)	120, 174
Eco53kI	GAGCTC	2 cut(s)	118, 172
Eco57I	CTGAAG	1 cut(s)	150
Eco81I	CCTNAGG	1 cut(s)	837
EcoICRI	GAGCTC	2 cut(s)	118, 172
EcoRI	GAATTC	1 cut(s)	402
EcoT14I	CCWWGG	4 cut(s)	51, 145, 380, 641
EcoT38I	GRGCYC	2 cut(s)	120, 174
ErhI	CCWWGG	4 cut(s)	51, 145, 380, 641
FaqI	GGGAC	1 cut(s)	622
FauI	CCCGC	1 cut(s)	590
FbaI	TGATCA	2 cut(s)	481, 855
Fnu4HI	GCNGC	3 cut(s)	471, 528, 687
FokI	GGATG	1 cut(s)	71
FriOI	GRGCYC	2 cut(s)	120, 174
Fsp4HI	GCNGC	3 cut(s)	471, 528, 687
FspBI	CTAG	2 cut(s)	227, 908
GlaI	GCGC	2 cut(s)	342, 620
GluI	GCNGC	3 cut(s)	471, 528, 687
GsuI	CTGGAG	1 cut(s)	374
HaeII	RGCGCY	1 cut(s)	344
HaeIII	GGCC	2 cut(s)	473, 573
HapII	CCGG	4 cut(s)	307, 321, 365, 823
HgaI	GACGC	1 cut(s)	202
HhaI	GCGC	2 cut(s)	343, 621
Hin6I	GCGC	2 cut(s)	341, 619
HinP1I	GCGC	2 cut(s)	341, 619
HincII	GTYRAC	1 cut(s)	24
HindII	GTYRAC	1 cut(s)	24
HindIII	AAGCTT	2 cut(s)	491, 1065
HinfI	GANTC	1 cut(s)	646
HpaI	GTTAAC	1 cut(s)	24
HpaII	CCGG	4 cut(s)	307, 321, 365, 823
HphI	GGTGA	6 cut(s)	194, 296, 734, 800, 865, 949
Hpy166II	GTNNAC	3 cut(s)	24, 232, 1019
Hpy188I	TCNGA	2 cut(s)	122, 918
Hpy188III	TCNNGA	4 cut(s)	217, 321, 479, 692
Hpy8I	GTNNAC	3 cut(s)	24, 232, 1019
Hpy99I	CGWCG	1 cut(s)	881
HpyAV	CCTTC	3 cut(s)	207, 844, 973
HpyCH4V	TGCA	5 cut(s)	287, 506, 539, 755, 801
HpyF10VI	GCNNNNNNNGC	3 cut(s)	150, 349, 536
HpyF3I	CTNAG	4 cut(s)	166, 185, 690, 837
HspAI	GCGC	2 cut(s)	341, 619
Kpn2I	TCCGGA	1 cut(s)	320
Ksp22I	TGATCA	2 cut(s)	481, 855
KspAI	GTTAAC	1 cut(s)	24
Kzo9I	GATC	7 cut(s)	193, 345, 481, 664, 700, 733, 855
LmnI	GCTCC	3 cut(s)	278, 459, 972
Lsp1109I	GCAGC	2 cut(s)	539, 673
LweI	GCATC	3 cut(s)	493, 742, 856
MaeI	CTAG	2 cut(s)	227, 908
MaeIII	GTNAC	2 cut(s)	722, 788
MalI	GATC	7 cut(s)	195, 347, 483, 666, 702, 735, 857
MboI	GATC	7 cut(s)	193, 345, 481, 664, 700, 733, 855
MboII	GAAGA	7 cut(s)	17, 44, 50, 53, 659, 767, 908
MfeI	CAATTG	2 cut(s)	135, 425
MhlI	GDGCHC	3 cut(s)	120, 174, 400
MluCI	AATT	6 cut(s)	135, 239, 266, 402, 425, 920
MmeI	TCCRAC	1 cut(s)	697
MroI	TCCGGA	1 cut(s)	320
MroXI	GAANNNNTTC	2 cut(s)	831, 993
MseI	TTAA	6 cut(s)	23, 392, 546, 657, 813, 1075
MspA1I	CMGCKG	1 cut(s)	530
MspCI	CTTAAG	1 cut(s)	391
MspI	CCGG	4 cut(s)	307, 321, 365, 823
MspR9I	CCNGG	1 cut(s)	824
MunI	CAATTG	2 cut(s)	135, 425
Mva1269I	GAATGC	1 cut(s)	801
MvnI	CGCG	4 cut(s)	95, 213, 705, 738
MwoI	GCNNNNNNNGC	3 cut(s)	150, 349, 536
NciI	CCSGG	1 cut(s)	824
NcoI	CCATGG	2 cut(s)	145, 380
NdeII	GATC	7 cut(s)	193, 345, 481, 664, 700, 733, 855
NheI	GCTAGC	1 cut(s)	907
NlaIV	GGNNCC	2 cut(s)	274, 378
NmuCI	GTSAC	2 cut(s)	722, 788
NspI	RCATGY	3 cut(s)	50, 677, 730
PagI	TCATGA	1 cut(s)	216
PceI	AGGCCT	1 cut(s)	573
PciI	ACATGT	1 cut(s)	726
PctI	GAATGC	1 cut(s)	801
PdmI	GAANNNNTTC	2 cut(s)	831, 993
PfeI	GAWTC	1 cut(s)	646
PkrI	GCNGC	3 cut(s)	472, 529, 688
PscI	ACATGT	1 cut(s)	726
Psp124BI	GAGCTC	2 cut(s)	120, 174
PspN4I	GGNNCC	2 cut(s)	274, 378
PstI	CTGCAG	1 cut(s)	541
PvuII	CAGCTG	1 cut(s)	530
RsaI	GTAC	2 cut(s)	225, 441
RsaNI	GTAC	2 cut(s)	224, 440
SacI	GAGCTC	2 cut(s)	120, 174
SaqAI	TTAA	6 cut(s)	23, 392, 546, 657, 813, 1075
SatI	GCNGC	3 cut(s)	471, 528, 687
Sau3AI	GATC	7 cut(s)	193, 345, 481, 664, 700, 733, 855
ScrFI	CCNGG	1 cut(s)	824
SduI	GDGCHC	3 cut(s)	120, 174, 400
SfaNI	GCATC	3 cut(s)	493, 742, 856
SfcI	CTRYAG	1 cut(s)	537
SmlI	CTYRAG	2 cut(s)	391, 575
SmoI	CTYRAG	2 cut(s)	391, 575
SpeI	ACTAGT	1 cut(s)	226
Sse9I	AATT	6 cut(s)	135, 239, 266, 402, 425, 920
SseBI	AGGCCT	1 cut(s)	573
SsiI	CCGC	5 cut(s)	95, 211, 470, 597, 671
SspI	AATATT	2 cut(s)	415, 1052
SspMI	CTAG	2 cut(s)	227, 908
SstI	GAGCTC	2 cut(s)	120, 174
StuI	AGGCCT	1 cut(s)	573
StyD4I	CCNGG	1 cut(s)	822
StyI	CCWWGG	4 cut(s)	51, 145, 380, 641
TaqI	TCGA	3 cut(s)	406, 630, 876
TasI	AATT	6 cut(s)	135, 239, 266, 402, 425, 920
TatI	WGTACW	1 cut(s)	439
TauI	GCSGC	1 cut(s)	473
TfiI	GAWTC	1 cut(s)	646
Tru1I	TTAA	6 cut(s)	23, 392, 546, 657, 813, 1075
Tru9I	TTAA	6 cut(s)	23, 392, 546, 657, 813, 1075
TscAI	CASTG	1 cut(s)	546
TseFI	GTSAC	2 cut(s)	722, 788
TseI	GCWGC	2 cut(s)	527, 686
Tsp45I	GTSAC	2 cut(s)	722, 788
TspDTI	ATGAA	1 cut(s)	767
TspRI	CASTG	1 cut(s)	546
Vha464I	CTTAAG	1 cut(s)	391
XapI	RAATTY	1 cut(s)	402
XceI	RCATGY	3 cut(s)	50, 677, 730
XmnI	GAANNNNTTC	2 cut(s)	831, 993
XspI	CTAG	2 cut(s)	227, 908

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.