MD05G1233800.v1.1

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005058
Physical Location & Seq
Forward (+)
36635128 .. 36635379
252 bp
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UTR
Exon/CDS
Intron
MD05G1233800.v1.1.491

Sequence Viewer

Length: 252 bp
ATGGAAGAGGAGAAAAGGATTATGCACTACAGCAGCTCTCAGAAGATATTGTTAGTAGGTGAGGGCAACTTTTCTTTTGCTGCTTCCTTAGCCACTGCATTTGGCTCTGCAAACAACATTGTCGCCACTTCTCTCGACTCCAAAGGTATTACGGGTGATAATCTATTTCGTTTTTGCTACATTGCACCGAGAATTCGCAATATTGATATCAAAAACCGTTTCAGAGTAGGTTCAACGATCAGAACCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

84

Amino Acids

9.23

Weight (kDa)

9.74

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 17 - 49 3.9e-10 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 192
AgsI TTSAA 1 cut(s) 234
AluBI AGCT 1 cut(s) 36
AluI AGCT 1 cut(s) 36
ApeKI GCWGC 2 cut(s) 33, 80
ApoI RAATTY 1 cut(s) 192
AsuHPI GGTGA 2 cut(s) 71, 167
BbvI GCAGC 2 cut(s) 45, 67
BfmI CTRYAG 1 cut(s) 28
BisI GCNGC 2 cut(s) 34, 81
BlsI GCNGC 2 cut(s) 35, 82
Bpu10I CCTNAGC 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 180
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 53
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 2 cut(s) 45, 67
Bsp143I GATC 1 cut(s) 237
BspCNI CTCAG 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 180
BssMI GATC 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 218
BstDEI CTNAG 2 cut(s) 39, 88
BstKTI GATC 1 cut(s) 240
BstMBI GATC 1 cut(s) 237
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstSFI CTRYAG 1 cut(s) 28
BstV1I GCAGC 2 cut(s) 45, 67
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviJI RGCY 3 cut(s) 36, 92, 105
CviKI_1 RGCY 3 cut(s) 36, 92, 105
DdeI CTNAG 2 cut(s) 39, 88
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
Eco32I GATATC 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 192
EcoRV GATATC 1 cut(s) 208
FaiI YATR 1 cut(s) 23
Fnu4HI GCNGC 2 cut(s) 34, 81
Fsp4HI GCNGC 2 cut(s) 34, 81
GluI GCNGC 2 cut(s) 34, 81
HinfI GANTC 1 cut(s) 137
HphI GGTGA 4 cut(s) 71, 167, 581, 1406
Hpy188I TCNGA 3 cut(s) 42, 224, 242
Hpy188III TCNNGA 1 cut(s) 134
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4V TGCA 4 cut(s) 25, 98, 110, 185
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 2 cut(s) 39, 88
Kzo9I GATC 1 cut(s) 237
Lsp1109I GCAGC 2 cut(s) 45, 67
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 2 cut(s) 17, 55
MluCI AATT 1 cut(s) 192
MlyI GAGTC 1 cut(s) 131
MnlI CCTC 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 89
NdeII GATC 1 cut(s) 237
PkrI GCNGC 2 cut(s) 35, 82
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
SatI GCNGC 2 cut(s) 34, 81
Sau3AI GATC 1 cut(s) 237
SchI GAGTC 1 cut(s) 131
SetI ASST 4 cut(s) 38, 61, 148, 232
SfcI CTRYAG 1 cut(s) 28
SgeI CNNG 3 cut(s) 146, 165, 201
Sse9I AATT 1 cut(s) 192
SspI AATATT 1 cut(s) 202
TaaI ACNGT 1 cut(s) 218
TaqI TCGA 1 cut(s) 135
TasI AATT 1 cut(s) 192
TscAI CASTG 1 cut(s) 100
TseI GCWGC 2 cut(s) 33, 80
TspRI CASTG 1 cut(s) 100
XapI RAATTY 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.