Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005058

Physical Location & Seq

Genomic Coordinates Reverse (-)

10231683 .. 10234569

Gene Structure 2887 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1133300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 708 bp

ATGCACCGTCATCCCACACTTAAGTCCATGAAATTTGATGTTATGATATTCAACTTTCCTCATGCAGGACATTACCATCGGCTGTGCGAAAAAGACGAGGAGCTCATAGAATTGCACAGGCATTTGTTAAAAGCCTTTTTCAAAAGCGCCCGCGGGATGCTCAGCAAAGGTGGTGAAATTCATGTTTCACACATGGTAGGCTACCCGTATGATCAATGGAAGTTGAAGGAGCTTGCTGAGAAAGCAGGCCTGTTTTTGAAAGAGAAAGTGTGGTTCAACAAGTCAGACTACCCTGGTTACCACAGTAAGAGGGGTGGCGGCATCCAGAGCGACAAAATGTTTCGTCCGAAAAAAAAAACATGTTACACCTTCAAATTTTCTCTGAAACAAACAAGTTCTGAACTTTCTGTTTGTAATCCCACAACCAGCATTGTAAGTTATGGCCCTTCACCACCTGGCTATCAGGGTTACATTTGTCCTCCAACTCACCCAAGCCCCACACTCTCTCCCCGCCTAGACGATTATGAACCTCCAGCTACTCCAAGCCCACCCTTACCCCCTTCCTGCCAAGACGATGATGACCCTCCAGCTCCCCCGAACCCGCCTTCACGCCAAGTTGATGAAGAAATCGGCTGCTACTCAGCCCTTAGAGACTGTTTGTCTTCAATTTTTCGCTGCTGGCCGGATATTGGACGCTCGCTGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008649 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016436 GO:0016740 GO:0016741 GO:0022613 GO:0031167 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0070042 GO:0070475 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:0140102 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF2431

Protein Analysis

236

Amino Acids

26.68

Weight (kDa)

8.54

Isoelectric Point (pI)

58.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BMT5-like	PF10354	2 - 104	2.6e-28	rRNA (uridine-N3-)-methyltransferase BTM5-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55790 AT1G55803
fragaria_vesca	FvH4_6g40260
malus_domestica	MD05G1233800.v1.1 MD09G1133300.v1.1 MD09G1133400.v1.1 MD09G1133600.v1.1 MD17G1120900.v1.1
prunus_persica	Prupe.3G193200_v2.0.a1 Prupe.3G193200_v2.0.a1 Prupe.3G193300_v2.0.a1 Prupe.6G197000_v2.0.a1
pyrus_communis	pycom09g05440 pycom09g05480 pycom17g11130
rosa_chinensis	RchiOBHm_Chr2g0155281 RchiOBHm_Chr2g0155291 RchiOBHm_Chr5g0024491
rosa_laevigata	RLG00000020835
rosa_multiflora	Rmu_sc0000761.1_g000011 Rmu_sc0012066.1_g000010
rosa_roxburghii	Rroxscaffold_2G00093790 Rroxscaffold_2G00093800
rosa_rugosa	Rorug02G0446700
rosa_samantha	Rh2AG513100 Rh2BG523700 Rh2CG498100 Rh2DG533400 Rh5BG172400
rosa_wichuraiana	Rw2G042160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	153
AciI	CCGC	5 cut(s)	151, 153, 318, 511, 602
AcoI	YGGCCR	1 cut(s)	680
AcsI	RAATTY	3 cut(s)	32, 177, 374
AfiI	CCNNNNNNNGG	2 cut(s)	65, 689
AflII	CTTAAG	1 cut(s)	20
AflIII	ACRYGT	1 cut(s)	359
AgsI	TTSAA	7 cut(s)	52, 142, 226, 259, 277, 373, 666
AhdI	GACNNNNNGTC	1 cut(s)	658
AjnI	CCWGG	2 cut(s)	292, 454
AluBI	AGCT	4 cut(s)	103, 232, 536, 590
AluI	AGCT	4 cut(s)	103, 232, 536, 590
Alw21I	GWGCWC	1 cut(s)	105
Alw26I	GTCTC	1 cut(s)	645
AoxI	GGCC	3 cut(s)	247, 442, 680
ApeKI	GCWGC	3 cut(s)	633, 675, 700
ApoI	RAATTY	3 cut(s)	32, 177, 374
AspLEI	GCGC	1 cut(s)	149
AspS9I	GGNCC	1 cut(s)	443
AsuHPI	GGTGA	3 cut(s)	185, 441, 479
BanII	GRGCYC	1 cut(s)	105
BbsI	GAAGAC	1 cut(s)	654
Bbv12I	GWGCWC	1 cut(s)	105
BbvI	GCAGC	3 cut(s)	620, 662, 687
BccI	CCATC	1 cut(s)	84
BciT130I	CCWGG	2 cut(s)	294, 456
BclI	TGATCA	1 cut(s)	211
BcoDI	GTCTC	1 cut(s)	645
BfaI	CTAG	1 cut(s)	515
BfoI	RGCGCY	1 cut(s)	150
BfrI	CTTAAG	1 cut(s)	20
BisI	GCNGC	4 cut(s)	319, 634, 676, 701
BlpI	GCTNAGC	1 cut(s)	161
BlsI	GCNGC	4 cut(s)	320, 635, 677, 702
Bme1390I	CCNGG	2 cut(s)	294, 456
BmeRI	GACNNNNNGTC	1 cut(s)	658
BmgT120I	GGNCC	1 cut(s)	443
BmrFI	CCNGG	2 cut(s)	294, 456
BmsI	GCATC	2 cut(s)	147, 330
BpiI	GAAGAC	1 cut(s)	654
BpmI	CTGGAG	2 cut(s)	516, 570
Bpu1102I	GCTNAGC	1 cut(s)	161
BsaJI	CCNNGG	2 cut(s)	151, 292
BsaXI	ACNNNNNCTCC	2 cut(s)	490, 520
Bsc4I	CCNNNNNNNGG	2 cut(s)	65, 689
BseBI	CCWGG	2 cut(s)	294, 456
BseDI	CCNNGG	2 cut(s)	151, 292
BseGI	GGATG	3 cut(s)	10, 162, 321
BseLI	CCNNNNNNNGG	2 cut(s)	65, 689
BseMII	CTCAG	3 cut(s)	175, 228, 654
BseRI	GAGGAG	1 cut(s)	113
BseXI	GCAGC	3 cut(s)	620, 662, 687
Bsh1236I	CGCG	1 cut(s)	153
BshFI	GGCC	3 cut(s)	249, 444, 682
BsiHKAI	GWGCWC	1 cut(s)	105
BsiSI	CCGG	1 cut(s)	683
BslI	CCNNNNNNNGG	2 cut(s)	65, 689
BsmAI	GTCTC	1 cut(s)	645
BsnI	GGCC	3 cut(s)	249, 444, 682
Bsp1286I	GDGCHC	1 cut(s)	105
Bsp143I	GATC	1 cut(s)	211
Bsp1720I	GCTNAGC	1 cut(s)	161
BspACI	CCGC	5 cut(s)	151, 153, 318, 511, 602
BspANI	GGCC	3 cut(s)	249, 444, 682
BspCNI	CTCAG	3 cut(s)	174, 229, 653
BspFNI	CGCG	1 cut(s)	153
BspTI	CTTAAG	1 cut(s)	20
BssECI	CCNNGG	2 cut(s)	151, 292
BssMI	GATC	1 cut(s)	211
Bst2UI	CCWGG	2 cut(s)	294, 456
Bst4CI	ACNGT	3 cut(s)	8, 305, 656
BstAFI	CTTAAG	1 cut(s)	20
BstC8I	GCNNGC	5 cut(s)	151, 234, 247, 680, 698
BstDEI	CTNAG	4 cut(s)	161, 237, 640, 647
BstDSI	CCRYGG	1 cut(s)	151
BstEII	GGTNACC	1 cut(s)	296
BstENI	CCTNNNNNAGG	1 cut(s)	63
BstF5I	GGATG	3 cut(s)	10, 162, 321
BstFNI	CGCG	1 cut(s)	153
BstH2I	RGCGCY	1 cut(s)	150
BstHHI	GCGC	1 cut(s)	149
BstKTI	GATC	1 cut(s)	214
BstMAI	GTCTC	1 cut(s)	645
BstMBI	GATC	1 cut(s)	211
BstMWI	GCNNNNNNNGC	2 cut(s)	242, 327
BstNI	CCWGG	2 cut(s)	294, 456
BstNSI	RCATGY	1 cut(s)	363
BstPI	GGTNACC	1 cut(s)	296
BstSCI	CCNGG	2 cut(s)	292, 454
BstUI	CGCG	1 cut(s)	153
BstV1I	GCAGC	3 cut(s)	620, 662, 687
BstV2I	GAAGAC	1 cut(s)	654
BsuRI	GGCC	3 cut(s)	249, 444, 682
BtgI	CCRYGG	1 cut(s)	151
BtsCI	GGATG	3 cut(s)	10, 162, 321
Cac8I	GCNNGC	5 cut(s)	151, 234, 247, 680, 698
CfoI	GCGC	1 cut(s)	149
Cfr13I	GGNCC	1 cut(s)	443
Cfr42I	CCGCGG	1 cut(s)	154
CseI	GACGC	1 cut(s)	702
CviAII	CATG	5 cut(s)	28, 62, 182, 193, 360
DdeI	CTNAG	4 cut(s)	161, 237, 640, 647
DpnI	GATC	1 cut(s)	213
DpnII	GATC	1 cut(s)	211
DriI	GACNNNNNGTC	1 cut(s)	658
EaeI	YGGCCR	1 cut(s)	680
Eam1105I	GACNNNNNGTC	1 cut(s)	658
Ecl136II	GAGCTC	1 cut(s)	103
Eco147I	AGGCCT	1 cut(s)	249
Eco24I	GRGCYC	1 cut(s)	105
Eco53kI	GAGCTC	1 cut(s)	103
Eco91I	GGTNACC	1 cut(s)	296
EcoICRI	GAGCTC	1 cut(s)	103
EcoNI	CCTNNNNNAGG	1 cut(s)	63
EcoO65I	GGTNACC	1 cut(s)	296
EcoRII	CCWGG	2 cut(s)	292, 454
EcoT38I	GRGCYC	1 cut(s)	105
FaeI	CATG	5 cut(s)	31, 65, 185, 196, 363
FatI	CATG	5 cut(s)	27, 61, 181, 192, 359
FauI	CCCGC	4 cut(s)	146, 158, 518, 609
FbaI	TGATCA	1 cut(s)	211
Fnu4HI	GCNGC	4 cut(s)	319, 634, 676, 701
FokI	GGATG	2 cut(s)	169, 308
FriOI	GRGCYC	1 cut(s)	105
Fsp4HI	GCNGC	4 cut(s)	319, 634, 676, 701
FspBI	CTAG	1 cut(s)	515
GlaI	GCGC	1 cut(s)	148
GluI	GCNGC	4 cut(s)	319, 634, 676, 701
GsuI	CTGGAG	2 cut(s)	516, 570
HaeII	RGCGCY	1 cut(s)	150
HaeIII	GGCC	3 cut(s)	249, 444, 682
HapII	CCGG	1 cut(s)	683
HgaI	GACGC	1 cut(s)	702
HhaI	GCGC	1 cut(s)	149
Hin1II	CATG	5 cut(s)	31, 65, 185, 196, 363
Hin6I	GCGC	1 cut(s)	147
HinP1I	GCGC	1 cut(s)	147
HpaII	CCGG	1 cut(s)	683
HphI	GGTGA	3 cut(s)	185, 441, 479
Hpy188I	TCNGA	4 cut(s)	286, 348, 384, 400
Hpy188III	TCNNGA	1 cut(s)	325
HpyAV	CCTTC	5 cut(s)	220, 379, 456, 570, 615
HpyCH4III	ACNGT	3 cut(s)	8, 305, 656
HpyCH4V	TGCA	3 cut(s)	4, 65, 115
HpyF10VI	GCNNNNNNNGC	2 cut(s)	242, 327
HpyF3I	CTNAG	4 cut(s)	161, 237, 640, 647
Hsp92II	CATG	5 cut(s)	31, 65, 185, 196, 363
HspAI	GCGC	1 cut(s)	147
Ksp22I	TGATCA	1 cut(s)	211
KspI	CCGCGG	1 cut(s)	154
Kzo9I	GATC	1 cut(s)	211
LmnI	GCTCC	3 cut(s)	100, 229, 595
Lsp1109I	GCAGC	3 cut(s)	620, 662, 687
LweI	GCATC	2 cut(s)	147, 330
MaeI	CTAG	1 cut(s)	515
MaeIII	GTNAC	3 cut(s)	296, 362, 467
MalI	GATC	1 cut(s)	213
MboI	GATC	1 cut(s)	211
MboII	GAAGA	2 cut(s)	635, 654
MhlI	GDGCHC	1 cut(s)	105
MluCI	AATT	5 cut(s)	32, 110, 177, 374, 666
MmeI	TCCRAC	1 cut(s)	506
MnlI	CCTC	6 cut(s)	69, 91, 303, 489, 540, 594
MseI	TTAA	2 cut(s)	21, 128
MspA1I	CMGCKG	1 cut(s)	153
MspCI	CTTAAG	1 cut(s)	20
MspI	CCGG	1 cut(s)	683
MspR9I	CCNGG	2 cut(s)	294, 456
MvaI	CCWGG	2 cut(s)	294, 456
MvnI	CGCG	1 cut(s)	153
MwoI	GCNNNNNNNGC	2 cut(s)	242, 327
NdeII	GATC	1 cut(s)	211
NlaIII	CATG	5 cut(s)	31, 65, 185, 196, 363
NspI	RCATGY	1 cut(s)	363
PceI	AGGCCT	1 cut(s)	249
PciI	ACATGT	1 cut(s)	359
PkrI	GCNGC	4 cut(s)	320, 635, 677, 702
PscI	ACATGT	1 cut(s)	359
Psp124BI	GAGCTC	1 cut(s)	105
Psp6I	CCWGG	2 cut(s)	292, 454
PspEI	GGTNACC	1 cut(s)	296
PspGI	CCWGG	2 cut(s)	292, 454
PspPI	GGNCC	1 cut(s)	443
SacI	GAGCTC	1 cut(s)	105
SacII	CCGCGG	1 cut(s)	154
SaqAI	TTAA	2 cut(s)	21, 128
SatI	GCNGC	4 cut(s)	319, 634, 676, 701
Sau3AI	GATC	1 cut(s)	211
Sau96I	GGNCC	1 cut(s)	443
ScrFI	CCNGG	2 cut(s)	294, 456
SduI	GDGCHC	1 cut(s)	105
SetI	ASST	8 cut(s)	105, 172, 234, 371, 457, 532, 538, 592
SfaNI	GCATC	2 cut(s)	147, 330
Sfr303I	CCGCGG	1 cut(s)	154
SgrBI	CCGCGG	1 cut(s)	154
SmlI	CTYRAG	1 cut(s)	20
SmoI	CTYRAG	1 cut(s)	20
Sse9I	AATT	5 cut(s)	32, 110, 177, 374, 666
SseBI	AGGCCT	1 cut(s)	249
SsiI	CCGC	5 cut(s)	151, 153, 318, 511, 602
SspMI	CTAG	1 cut(s)	515
SstI	GAGCTC	1 cut(s)	105
StuI	AGGCCT	1 cut(s)	249
StyD4I	CCNGG	2 cut(s)	292, 454
TaaI	ACNGT	3 cut(s)	8, 305, 656
TasI	AATT	5 cut(s)	32, 110, 177, 374, 666
TauI	GCSGC	1 cut(s)	321
Tru1I	TTAA	2 cut(s)	21, 128
Tru9I	TTAA	2 cut(s)	21, 128
TseI	GCWGC	3 cut(s)	633, 675, 700
TspDTI	ATGAA	4 cut(s)	44, 170, 540, 636
Vha464I	CTTAAG	1 cut(s)	20
XagI	CCTNNNNNAGG	1 cut(s)	63
XapI	RAATTY	3 cut(s)	32, 177, 374
XceI	RCATGY	1 cut(s)	363
XspI	CTAG	1 cut(s)	515

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.