RLG00000020835

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005058
Physical Location & Seq
Forward (+)
69222325 .. 69224977
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020835

Sequence Viewer

Length: 759 bp
ATGGAGAGATGGATTGAGCACTACTCTAGCTCACAGCAGATACTGCTGGTTGGGGAAGGCGACTTCTCATTCTCTGCTTGCTTGGCTCGAGCTTTTGGCTACGCGCCTAACATGGTTGCTACTTCTCTCGACTCCCAAGAATCTCTGTGGGAAAAGCATTGGAGCTGTGTGTCACACTTGGATGAGTTGAGGGGGAGAGGATGCCTAGTGTTGCATGATGTGAATGTGCATGACATGGACCTCCATTACATCCTGAAATGGAAGAAATTTGATGTTATCATCTTCAACTTTCCTCATGCAGGGCATTACCCTTGGCTCCGTGAGAGAGATAATCAGCTCATTCAGATGCACAGGCAATTGCTCAGTGGGTTTTTTGCAAGCGCCCGAGTGATGCTTCACTCTGGTGGTCAAATTCATGTGACAACCAGGGATGACTACCCATATAACATATGGAATGTGGAGGAACTTGCCGCTCAAGCAGGCCTGGTTTTGAGAGAGAAGGTGTGGTTCGAAAAGTCTGACTACCCTGGCTATCACAATAAGAGAGGCGGTGGCATTGACAGCAACAAGAAATTTCCACTGGGAGAGAGCTACACCTTCAAATTTTTAATGAAGACGGAAGAAGATGGGGTCTGCAAGCTGCCGACGGCTCGCGATACCAGCGAACGACCGTCTCATTTCCCCTTACTGGTGAAACCAGAGTCTCTACATAAGGTTGAAGCAACCAGCCCGGATTGCGAGTCTCATGAACATACCTAA

Protein Analysis

253

Amino Acids

29.12

Weight (kDa)

6.13

Isoelectric Point (pI)

57.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 15 - 182 2.5e-51 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 473
AccII CGCG 2 cut(s) 104, 654
AciI CCGC 2 cut(s) 471, 549
AcsI RAATTY 4 cut(s) 266, 411, 572, 602
AfiI CCNNNNNNNGG 2 cut(s) 299, 688
AgsI TTSAA 3 cut(s) 286, 601, 719
AjnI CCWGG 3 cut(s) 425, 483, 526
AleI CACNNNNGTG 1 cut(s) 402
AluBI AGCT 6 cut(s) 30, 92, 165, 337, 591, 640
AluI AGCT 6 cut(s) 30, 92, 165, 337, 591, 640
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 678, 708, 747
AlwNI CAGNNNCTG 1 cut(s) 43
Ama87I CYCGRG 2 cut(s) 87, 384
AoxI GGCC 1 cut(s) 481
ApeKI GCWGC 1 cut(s) 640
ApoI RAATTY 4 cut(s) 266, 411, 572, 602
AspLEI GCGC 2 cut(s) 106, 383
AspS9I GGNCC 1 cut(s) 238
AsuC2I CCSGG 1 cut(s) 731
AsuHPI GGTGA 1 cut(s) 703
AsuII TTCGAA 1 cut(s) 510
AvaI CYCGRG 2 cut(s) 87, 384
AvaII GGWCC 1 cut(s) 238
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 1 cut(s) 627
BccI CCATC 2 cut(s) 3, 620
BceAI ACGGC 1 cut(s) 663
BciT130I CCWGG 3 cut(s) 427, 485, 528
BcnI CCSGG 1 cut(s) 731
BcoDI GTCTC 3 cut(s) 678, 708, 747
BfaI CTAG 2 cut(s) 27, 206
BfoI RGCGCY 1 cut(s) 384
BisI GCNGC 2 cut(s) 471, 641
BlsI GCNGC 2 cut(s) 472, 642
Bme1390I CCNGG 4 cut(s) 427, 485, 528, 731
Bme18I GGWCC 1 cut(s) 238
BmeT110I CYCGRG 2 cut(s) 87, 384
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 1 cut(s) 317
BmrFI CCNGG 4 cut(s) 427, 485, 528, 731
BmrI ACTGGG 1 cut(s) 590
BmsI GCATC 3 cut(s) 191, 336, 381
BmuI ACTGGG 1 cut(s) 590
BpiI GAAGAC 1 cut(s) 620
BplI GAGNNNNNCTC 2 cut(s) 8, 40
Bpu14I TTCGAA 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 731
BsaJI CCNNGG 3 cut(s) 311, 426, 526
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 688
Bse1I ACTGG 2 cut(s) 585, 693
BseBI CCWGG 3 cut(s) 427, 485, 528
BseDI CCNNGG 3 cut(s) 311, 426, 526
BseGI GGATG 4 cut(s) 187, 206, 249, 436
BseLI CCNNNNNNNGG 2 cut(s) 299, 688
BseMII CTCAG 1 cut(s) 376
BseNI ACTGG 2 cut(s) 585, 693
BseXI GCAGC 1 cut(s) 627
Bsh1236I CGCG 2 cut(s) 104, 654
Bsh1285I CGRYCG 1 cut(s) 671
BshFI GGCC 1 cut(s) 483
BsiEI CGRYCG 1 cut(s) 671
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 2 cut(s) 87, 384
BsiSI CCGG 1 cut(s) 731
BslI CCNNNNNNNGG 2 cut(s) 299, 688
BsmAI GTCTC 3 cut(s) 678, 708, 747
BsmBI CGTCTC 1 cut(s) 678
BsnI GGCC 1 cut(s) 483
BsoBI CYCGRG 2 cut(s) 87, 384
Bsp119I TTCGAA 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 21
Bsp68I TCGCGA 1 cut(s) 654
BspACI CCGC 2 cut(s) 471, 549
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 1 cut(s) 375
BspFNI CGCG 2 cut(s) 104, 654
BspHI TCATGA 1 cut(s) 745
BspLI GGNNCC 1 cut(s) 317
BspT104I TTCGAA 1 cut(s) 510
BsrBI CCGCTC 1 cut(s) 473
BsrI ACTGG 2 cut(s) 585, 693
BssECI CCNNGG 3 cut(s) 311, 426, 526
BssT1I CCWWGG 1 cut(s) 311
Bst2UI CCWGG 3 cut(s) 427, 485, 528
Bst4CI ACNGT 1 cut(s) 672
BstAPI GCANNNNNTGC 1 cut(s) 43
BstBI TTCGAA 1 cut(s) 510
BstC8I GCNNGC 5 cut(s) 79, 379, 481, 638, 652
BstDEI CTNAG 1 cut(s) 362
BstENI CCTNNNNNAGG 1 cut(s) 297
BstF5I GGATG 4 cut(s) 187, 206, 249, 436
BstFNI CGCG 2 cut(s) 104, 654
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 2 cut(s) 106, 383
BstMAI GTCTC 3 cut(s) 678, 708, 747
BstMCI CGRYCG 1 cut(s) 671
BstMWI GCNNNNNNNGC 6 cut(s) 43, 83, 476, 561, 660, 735
BstNI CCWGG 3 cut(s) 427, 485, 528
BstSCI CCNGG 4 cut(s) 425, 483, 526, 729
BstUI CGCG 2 cut(s) 104, 654
BstV1I GCAGC 1 cut(s) 627
BstV2I GAAGAC 1 cut(s) 620
BsuRI GGCC 1 cut(s) 483
BtsCI GGATG 4 cut(s) 187, 206, 249, 436
BtsIMutI CAGTG 2 cut(s) 370, 578
BtuMI TCGCGA 1 cut(s) 654
Cac8I GCNNGC 5 cut(s) 79, 379, 481, 638, 652
CaiI CAGNNNCTG 1 cut(s) 43
CciI TCATGA 1 cut(s) 745
CfoI GCGC 2 cut(s) 106, 383
Cfr13I GGNCC 1 cut(s) 238
CviAII CATG 7 cut(s) 112, 215, 230, 235, 296, 416, 746
DdeI CTNAG 1 cut(s) 362
Eco130I CCWWGG 1 cut(s) 311
Eco147I AGGCCT 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 238
Eco88I CYCGRG 2 cut(s) 87, 384
EcoNI CCTNNNNNAGG 1 cut(s) 297
EcoRII CCWGG 3 cut(s) 425, 483, 526
EcoT14I CCWWGG 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 311
Esp3I CGTCTC 1 cut(s) 678
FaeI CATG 7 cut(s) 115, 218, 233, 238, 299, 419, 749
FatI CATG 7 cut(s) 111, 214, 229, 234, 295, 415, 745
FauNDI CATATG 1 cut(s) 449
Fnu4HI GCNGC 2 cut(s) 471, 641
FokI GGATG 4 cut(s) 194, 213, 236, 443
Fsp4HI GCNGC 2 cut(s) 471, 641
FspBI CTAG 2 cut(s) 27, 206
GlaI GCGC 2 cut(s) 105, 382
GluI GCNGC 2 cut(s) 471, 641
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 1 cut(s) 483
HapII CCGG 1 cut(s) 731
HhaI GCGC 2 cut(s) 106, 383
Hin1II CATG 7 cut(s) 115, 218, 233, 238, 299, 419, 749
Hin6I GCGC 2 cut(s) 104, 381
HinP1I GCGC 2 cut(s) 104, 381
HinfI GANTC 4 cut(s) 131, 140, 701, 740
HpaII CCGG 1 cut(s) 731
HphI GGTGA 1 cut(s) 703
Hpy188I TCNGA 2 cut(s) 345, 520
Hpy188III TCNNGA 4 cut(s) 128, 253, 653, 746
Hpy99I CGWCG 1 cut(s) 649
HpyAV CCTTC 3 cut(s) 50, 493, 607
HpyCH4III ACNGT 1 cut(s) 672
HpyCH4V TGCA 6 cut(s) 214, 229, 299, 349, 377, 636
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 83, 476, 561, 660, 735
HpyF3I CTNAG 1 cut(s) 362
Hsp92II CATG 7 cut(s) 115, 218, 233, 238, 299, 419, 749
HspAI GCGC 2 cut(s) 104, 381
LmnI GCTCC 2 cut(s) 162, 321
Lsp1109I GCAGC 1 cut(s) 627
LweI GCATC 3 cut(s) 191, 336, 381
MaeI CTAG 2 cut(s) 27, 206
MaeIII GTNAC 2 cut(s) 171, 418
MbiI CCGCTC 1 cut(s) 473
MboII GAAGA 5 cut(s) 274, 274, 625, 632, 635
MfeI CAATTG 1 cut(s) 356
MhlI GDGCHC 1 cut(s) 21
MluCI AATT 5 cut(s) 266, 356, 411, 572, 602
MlyI GAGTC 3 cut(s) 125, 710, 749
MnlI CCTC 6 cut(s) 183, 191, 251, 303, 454, 539
MseI TTAA 1 cut(s) 608
MslI CAYNNNNRTG 3 cut(s) 180, 344, 402
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 4 cut(s) 427, 485, 528, 731
MunI CAATTG 1 cut(s) 356
MvaI CCWGG 3 cut(s) 427, 485, 528
MvnI CGCG 2 cut(s) 104, 654
MwoI GCNNNNNNNGC 6 cut(s) 43, 83, 476, 561, 660, 735
NciI CCSGG 1 cut(s) 731
NdeI CATATG 1 cut(s) 449
NlaIII CATG 7 cut(s) 115, 218, 233, 238, 299, 419, 749
NlaIV GGNNCC 1 cut(s) 317
NmuCI GTSAC 2 cut(s) 171, 418
NruI TCGCGA 1 cut(s) 654
NspV TTCGAA 1 cut(s) 510
OliI CACNNNNGTG 1 cut(s) 402
PaeR7I CTCGAG 1 cut(s) 87
PagI TCATGA 1 cut(s) 745
PceI AGGCCT 1 cut(s) 483
PfeI GAWTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 472, 642
PleI GAGTC 3 cut(s) 125, 709, 748
PpsI GAGTC 3 cut(s) 125, 709, 748
Psp6I CCWGG 3 cut(s) 425, 483, 526
PspGI CCWGG 3 cut(s) 425, 483, 526
PspN4I GGNNCC 1 cut(s) 317
PspPI GGNCC 1 cut(s) 238
PspXI VCTCGAGB 1 cut(s) 87
PstNI CAGNNNCTG 1 cut(s) 43
RruI TCGCGA 1 cut(s) 654
RseI CAYNNNNRTG 3 cut(s) 180, 344, 402
SaqAI TTAA 1 cut(s) 608
SatI GCNGC 2 cut(s) 471, 641
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 3 cut(s) 125, 710, 749
ScrFI CCNGG 4 cut(s) 427, 485, 528, 731
SduI GDGCHC 1 cut(s) 21
SfaNI GCATC 3 cut(s) 191, 336, 381
Sfr274I CTCGAG 1 cut(s) 87
SfuI TTCGAA 1 cut(s) 510
SinI GGWCC 1 cut(s) 238
SlaI CTCGAG 1 cut(s) 87
SmiMI CAYNNNNRTG 3 cut(s) 180, 344, 402
SmlI CTYRAG 2 cut(s) 87, 474
SmoI CTYRAG 2 cut(s) 87, 474
Sse9I AATT 5 cut(s) 266, 356, 411, 572, 602
SseBI AGGCCT 1 cut(s) 483
SsiI CCGC 2 cut(s) 471, 549
SspMI CTAG 2 cut(s) 27, 206
StuI AGGCCT 1 cut(s) 483
StyD4I CCNGG 4 cut(s) 425, 483, 526, 729
StyI CCWWGG 1 cut(s) 311
TaaI ACNGT 1 cut(s) 672
TaqI TCGA 3 cut(s) 88, 129, 510
TasI AATT 5 cut(s) 266, 356, 411, 572, 602
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 1 cut(s) 608
Tru9I TTAA 1 cut(s) 608
TscAI CASTG 2 cut(s) 370, 585
TseFI GTSAC 2 cut(s) 171, 418
TseI GCWGC 1 cut(s) 640
Tsp45I GTSAC 2 cut(s) 171, 418
TspDTI ATGAA 2 cut(s) 404, 626
TspGWI ACGGA 2 cut(s) 308, 632
TspRI CASTG 2 cut(s) 370, 585
VpaK11BI GGWCC 1 cut(s) 238
XagI CCTNNNNNAGG 1 cut(s) 297
XapI RAATTY 4 cut(s) 266, 411, 572, 602
XcmI CCANNNNNNNNNTGG 1 cut(s) 447
XhoI CTCGAG 1 cut(s) 87
XspI CTAG 2 cut(s) 27, 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.