Rh5BG172400

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0005058
Physical Location & Seq
Forward (+)
18055561 .. 18056271
711 bp
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UTR
Exon/CDS
Intron
Rh5BG172400.1

Sequence Viewer

Length: 273 bp
ATGGAAGAGGCTATAGCAGATACTAAACGAGAGAAAAGGATTATGCATTACAGCAGCAATCAGAAGATACTGTTAGTGGGTGAGGGCAACTTCTCTTTTGCTGCTTGTTTAGCCAAAGAGTTTGGCTCAGCGAAGAACATGGTTGCCACTTCTCTCGACTCCAAAGGTAATAAGAATGTGATACGGAAGAACAATATTGAGACACATCAAAACTTTAGAGGACCAAACGTTGTTCAAATGTACAACAGAACCGACAAAGCTACAAAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

90

Amino Acids

10.14

Weight (kDa)

9.72

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 24 - 57 2.9e-10 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 228
AfaI GTAC 1 cut(s) 242
AgsI TTSAA 1 cut(s) 236
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
Alw26I GTCTC 1 cut(s) 194
ApeKI GCWGC 2 cut(s) 54, 101
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 92
AvaII GGWCC 1 cut(s) 221
BbvI GCAGC 2 cut(s) 66, 88
BcoDI GTCTC 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 12
BisI GCNGC 2 cut(s) 55, 102
BlpI GCTNAGC 1 cut(s) 127
BlsI GCNGC 2 cut(s) 56, 103
Bme18I GGWCC 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 221
BplI GAGNNNNNCTC 2 cut(s) 110, 142
Bpu1102I GCTNAGC 1 cut(s) 127
BseMII CTCAG 1 cut(s) 141
BseXI GCAGC 2 cut(s) 66, 88
BsmAI GTCTC 1 cut(s) 194
Bsp1407I TGTACA 1 cut(s) 240
Bsp1720I GCTNAGC 1 cut(s) 127
BspCNI CTCAG 1 cut(s) 140
BsrGI TGTACA 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 72
BstAUI TGTACA 1 cut(s) 240
BstDEI CTNAG 1 cut(s) 127
BstMAI GTCTC 1 cut(s) 194
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 66, 88
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 1 cut(s) 241
CviAII CATG 1 cut(s) 139
CviJI RGCY 4 cut(s) 11, 113, 126, 260
CviKI_1 RGCY 4 cut(s) 11, 113, 126, 260
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 1 cut(s) 127
Eco47I GGWCC 1 cut(s) 221
EcoT22I ATGCAT 1 cut(s) 48
FaeI CATG 1 cut(s) 142
FaiI YATR 4 cut(s) 14, 44, 140, 271
FatI CATG 1 cut(s) 138
Fnu4HI GCNGC 2 cut(s) 55, 102
Fsp4HI GCNGC 2 cut(s) 55, 102
GluI GCNGC 2 cut(s) 55, 102
Hin1II CATG 1 cut(s) 142
HinfI GANTC 1 cut(s) 158
HphI GGTGA 1 cut(s) 92
Hpy188I TCNGA 1 cut(s) 63
Hpy188III TCNNGA 1 cut(s) 155
HpyCH4III ACNGT 1 cut(s) 72
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 1 cut(s) 46
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 1 cut(s) 127
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 1 cut(s) 142
Lsp1109I GCAGC 2 cut(s) 66, 88
MaeII ACGT 1 cut(s) 228
MboII GAAGA 4 cut(s) 17, 76, 145, 199
MlyI GAGTC 1 cut(s) 152
MnlI CCTC 2 cut(s) 76, 212
Mph1103I ATGCAT 1 cut(s) 48
MwoI GCNNNNNNNGC 1 cut(s) 110
NlaIII CATG 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 48
PkrI GCNGC 2 cut(s) 56, 103
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Psp1406I AACGTT 1 cut(s) 228
PspPI GGNCC 1 cut(s) 221
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
SatI GCNGC 2 cut(s) 55, 102
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 1 cut(s) 152
SetI ASST 3 cut(s) 169, 231, 262
SfcI CTRYAG 1 cut(s) 12
SgeI CNNG 4 cut(s) 41, 117, 151, 167
SinI GGWCC 1 cut(s) 221
SspI AATATT 1 cut(s) 196
TaaI ACNGT 1 cut(s) 72
TaiI ACGT 1 cut(s) 231
TaqI TCGA 1 cut(s) 156
TatI WGTACW 1 cut(s) 240
TseI GCWGC 2 cut(s) 54, 101
TspGWI ACGGA 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 221
Zsp2I ATGCAT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.