MD05G1251600.v1.1

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003630
Physical Location & Seq
Forward (+)
38326370 .. 38331950
5581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1251600.v1.1.491

Sequence Viewer

Length: 4455 bp
ATGGTTGCTATGAATAGTGAATTTGACAGCTTTTTGTTAAGACCACCAATGGAGTCAGACGAGGAGCAGGATTGTTTTTGCGGGTTATATCCGGAGTATGGTGCAATTTTCATGTCAAGCAGTGCAACGATAGATGAGTGTTTTGAACGGAGACTGTTTGGCCTTCCATCTTCACAAGGCCAATTTGTAAAGCAGATTAAAACTGGAATGATTTTGTTTCTGTTTGAATTCGAGAGGAGAGAACTTCATGGTGTTTTTCAAGCATGCTCTGATGGTGAAATGAATATTTTGCCTTCTGCATACAATTCATCAGGAAGGCAGTTTCCTGCTCAGGTAAAAGTGAAGCTTATTTGGCGTTGTCATTCGCTTTCAGAACCTGAATTCAGTGGCGCAATCAAAGATAACTACTTTTCAAATTGGAAGTTCCGTTTCGGTCTCTCCAAAGCTCAGGTCCGAAGACTTTTATTGTTGTTCAGCTCACGAAAGTTAAAATATCAGCAGCGGCAGAGACAATTAGCCAGTAGAAGAGAACCAATATCAGTGGACACTGCTGGTAGAGTCAAGGAAGTCGATGATGGCAAGTCTGTATTGAGTGATAAGGCAATCAACAAGCTTGTTGCCGACAATCATAATGGGCCAAAAATGAGTCAGTCACCGTGGAATGTCTCTGGGAATATTGGAAAAGAAGATGGTGGCATGTTGGCAAGTGATGAAGTGGGTAATCTTCATGAAGTAGACAAGAAGGTTGGGAAGATCATGCAGCGTGTGTATCTTGGGCCTGTTCCAGGTGAAGTTGGAAAATTGGATGCTGCTTTTGCAATGTCTGAGAGGGCAGGGAATGAATTTAATGTGGATGTTGAATCTGTGTCATCTGAGCATCCTATGTCGGATCAATCTGGACGAGGTGATGACGATCTGGTAATTCATGATGATTCTAGCTGTTTAATTGGTGATAAATTACAAATTGGCAATAGTGAAAGTAATGTTTTTGGGTCAGCTGTACCTACTGCGAACCCTGCTTTCTTTCAGTCTAGTCTTGATAGACCTGTCTGTCCTGGCTTGCCTGTTGAAGAAACGGGTTCTTTGGTTCAAGATCAAACTAGATCAACTTCGACTGTTGGCAATCTAATGGAGCTGCCAATTGCTAGCCATATTTGTTCCGCATCTCCATCTTATAGAGATGCGATTGTAATCAGCACCTATGATCCTGATGCTCTTAGTCTGAACAATCCACGCTCACCATCACTTGGGGTCAATCAGAGTTCAAATTCCGTTCAATATTGTTATAAACAACATGGCATTCTTTCCATTACAAATCAATCATATACAGAGCTAAAAGGAATCAATCAAAGCCTAGAATCCAATCCAAAGTTTGATGTTCATGTTCCAATCGCATCCCATGACCTTTACGAGCACTCATGTCACGGGGACATCATGCCTTTTCCAGGGCTGGCATATTCTGAAGAATTGGCTATAGATTCTTCTGGAAAACATTGTTCCGGGGAGCCAGTTTTGAACAGTTCTTTATCAATTGAAAGTTTATCACAAATTGGAAGTAGTGGGACGGAAACCAAGAGGCCAACATCTTATTCTCTCAGTCCTAGTAACTGTCTGTCCTTTGTGATTGATCGTGGTCATCCTGTAGCATTACAAAAGAAACTTGAGCAAGAAATGGCACAGAAATTGAACGATGAACCATGTACTGCTAATGTAACTTCATCAGAGGAGCTTCAGTTCCAGTGGCAATGTCGCATTCATCCTGATGATCACAGCGTGGACCACCATATACAGAAATCTTATGATCGTGATCTCAATTCTTATGCATTCTCTGAAGGTAAGCATTCTGATCTGAAGAATGAAAGAAGTGTATTTTCTCGCTTGGCTTCAGATACGCCTGGCAAAGATAACAGTATGCATGCTGACAACGAGGAAAGTGATAACGATGCATCAGTGGATGAAGTCATGGCAATGTTGAGTGCGAGTAACTACAGCTGGGTGAAGTCAAAAAATCCTAAGCGGCCTACTAGACGGCACGATGACCAAGAGAAGTTTAGGAATAAGAAAAAGACCAAAGTGGACTCTGAGTTGGATAGTAATTACTTGGAATTGATTCCAAAAAAATCAAACATGGCTTCTGCTACACCTGTTGAAGACAAAGATGATCAAAGGCGCGAAGAGGTACCATTTGTGGATTTCAAGCGACGGAGTGAACTGCAGAAACTTAATGGTGATACCAAAGCCAGAGCCAAGGATGAAATTGCAGAAAATGATAGACTGTTGGGTGGGCAGTGCAAGAGAAGGAAGTTGGTCAGGCCGAGTTTCAATGACAACGAGCCACACGATGAAAAAAGCATGAATGAAAACCCATCTCTGATTTTACAAGCATCATCACAAGAATCTTCTGTTAGCGAAGATGTTAATGGCAAGAGAAGGAAGCTGGTTAGACCAAAGTTCAGGGTGAATGAGCCATGTGATGACAAAAGCATGAATGGAGACACTTCTAAAATTTTACAAGTATCATCTCAACACTGTTGTACTAGTGAAGATAGTGGAAGTTGCGAGGCTTCTGTTGGAAGCCAAGGTAAGTTGTTACCGCAAGTTATAGGTTTGTCGCTTATGGTGTGTCAAAGCAGTCATGAGAATGAGAATAATCAAACCGAAAGATCTTCAAAACATGAAAGAGAAATTGAAGCTGAAAGCTCCGCAGCATCAGCCAAATTAGGAGATGAAGGTCGAAAAGAGCCTCTTCATGATGTTGGCAGCCAAATTGCTGACCTTGCAATCCCTAAGTTGTTACCGCAATGTACAGAATCGCCTCAAGTGGTTCGTGAAGGTCAAAAAGAGCTTCTTCATGACGTTGGCAGCCAAATTGCTAACCTTGCAATTCTGTTACCGCAATATACAGAATCACCTCAAGTAGTTCATCAAAGCAGTTGTGAGAATGAGAATATTGAAACGGAAAGATTTCAAGAATATGAGCTGGAAATTAAAACAGAAATTTCCAGACGGTCTCTCAAACTTGGAGATGGTGGTGGAGTGGAGCCTCTTCATGATCTTGGCAGCCCAATTGGCAGCCTTGCAATCCCATATGGAGACAACAATCCTAATGTTCAGAAAGACTCGGATTATAAGGTTCCTATTGTAAACAAATCATCTCAAGATTGTAATGATATCAGTCACATCACTGTTCAGGAGTCAGCCCTGCGATTACCTGAACAGAATTCTGGAACAGAAAATGCATTTTCTGAAATTGAATGTGCAAGAGTCAATGAACTGGAGTTGCTTCCAAGCGTTGAACTTTGTCCTGAAAGTGGTGATCTTAGTAGCGGTAATGCAATAGGCAGCTCAAGTAATCTAAACTCTGGGTTGCAAAGAGGAACCAAAGAAGTTGCTATTTGTGTATATGCTGGTGTTGGTGATAACCACCATGAATCCAGTAATAAACAATCTGAGGCATCTCTTGACACCGTGGGTTGCTTGGACAAGCAAGAACCGTCTCAAGATCACAGATCCAGTAAATTGTCTGAGGCATCTTTTGGCACTGTCGGTTGCTTCAAAAAGCCAGAATCTTCTCTAGAATTTCGGGACCTGAATTTGGGATTTGGAGAGCGACTTTTTATACTTAGTAATAGTTTCAAAAGCTCTGATGTTACTGCTTCCAAGCTTGTGGGTGAAAGGAGAGATGGTCTTCAAATCAGCGGTAGTAATGTTTGCAAAAGTTCCGACATTGATACTTCTGAGATTGTGGAGGATACGATTAGCAAGGATCTTGGTCCAAGCAGAAAACTTGATCTCAAGGCAGCCGACAACCGCTCTGATATTGATACTTCTAAGCATGTGGGCGATGCACAGGATTCTCTTCCAATCAGCGGTAGTAATGTTTGCAAAAGCATTAGTACCTCTGAGAATGTATGCAAGGGTATAATTAGAAAACGTGTACTGAAGAGAGCAATCAACTGCTCTAAATTTGATACTTCTATGCATGTGGACGATGTGAAGGATGTTCATCCAATCCGCGACAGTAATGTTGACAAAAGCATTGGTACCTCCGAGGATGTGGAAGAGAAGAAGGTTATTTTCCCAAGCAGGGCTATCATTAGAAAACGTGTACTCAAGATAGCAGACAACTGCTCTAAAATTGATACTTCTAAGCACGTGGACGATGCAAAGGATTTTCTCCCAATCAGCGGCAGTAATTCTTGCAAAAGCTCAGACGGTGCTACCTCTGAGAGTGCGGAAGCAAGGGAGGATGTTCTTCAAAGCAACGTTGAAATCGGAAAAGGTGAACTCAAGACGGTCTGCAGCCAAAATACAAGTTTAGCTCATGATTTTGAGTTTGATGATGATAGTAAAACTGATGATACTAGAAGAGAAAACAAGAGGCTTTGGAGTATTCTAACGGCCAGGTTGAAGCAGCTGAAGAATGCTCAACAAACAACAGTGACAAGGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

1485

Amino Acids

163.68

Weight (kDa)

5.29

Isoelectric Point (pI)

55.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 34 - 158 5.4e-46 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1287, 3129
AasI GACNNNNNNGTC 1 cut(s) 1050
Acc65I GGTACC 2 cut(s) 2179, 4043
AccB1I GGYRCC 2 cut(s) 2179, 4043
AccB7I CCANNNNNTGG 1 cut(s) 1247
AccBSI CCGCTC 1 cut(s) 3813
AccI GTMKAC 1 cut(s) 735
AccII CGCG 2 cut(s) 2172, 4017
AccIII TCCGGA 1 cut(s) 91
AclI AACGTT 1 cut(s) 4266
AclWI GGATC 4 cut(s) 897, 1199, 3504, 3774
AcoI YGGCCR 1 cut(s) 4401
AcuI CTGAAG 7 cut(s) 1482, 1715, 1851, 1869, 1871, 3962, 4439
AcvI CACGTG 1 cut(s) 4156
AdeI CACNNNGTG 1 cut(s) 1774
AfaI GTAC 9 cut(s) 1002, 1702, 2181, 2536, 2806, 3898, 3939, 4045, 4110
AfiI CCNNNNNNNGG 9 cut(s) 98, 785, 1247, 1445, 3311, 3595, 3869, 4087, 4187
AflIII ACRYGT 2 cut(s) 3934, 4105
AhlI ACTAGT 1 cut(s) 2537
AjnI CCWGG 5 cut(s) 784, 1054, 1444, 1894, 4403
AjuI GAANNNNNNNTTGG 2 cut(s) 1573, 1605
AloI GAACNNNNNNTCC 2 cut(s) 1718, 1750
Alw21I GWGCWC 1 cut(s) 1416
Alw26I GTCTC 8 cut(s) 145, 440, 502, 670, 2487, 3015, 3087, 3501
AlwI GGATC 4 cut(s) 897, 1199, 3504, 3774
AlwNI CAGNNNCTG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 91
AoxI GGCC 8 cut(s) 160, 178, 635, 776, 1577, 2018, 2312, 4401
ArsI GACNNNNNNTTYG 2 cut(s) 435, 467
Asp700I GAANNNNTTC 2 cut(s) 2109, 2964
Asp718I GGTACC 2 cut(s) 2179, 4043
AspLEI GCGC 2 cut(s) 392, 2172
AspS9I GGNCC 7 cut(s) 451, 635, 776, 1777, 3586, 3773, 4449
AsuC2I CCSGG 1 cut(s) 1501
AsuNHI GCTAGC 1 cut(s) 1145
AvaII GGWCC 5 cut(s) 451, 1777, 3586, 3773, 4449
BanI GGYRCC 2 cut(s) 2179, 4043
BbrPI CACGTG 1 cut(s) 4156
BbsI GAAGAC 3 cut(s) 463, 2157, 3680
Bbv12I GWGCWC 1 cut(s) 1416
BccI CCATC 9 cut(s) 175, 266, 569, 683, 1177, 1249, 2374, 3020, 3677
BceAI ACGGC 2 cut(s) 2045, 4416
BciT130I CCWGG 5 cut(s) 786, 1056, 1446, 1896, 4405
BciVI GTATCC 1 cut(s) 3745
BclI TGATCA 2 cut(s) 1765, 2161
BcnI CCSGG 1 cut(s) 1501
BcoDI GTCTC 8 cut(s) 145, 440, 502, 670, 2487, 3015, 3087, 3501
BcuI ACTAGT 1 cut(s) 2537
BfmI CTRYAG 5 cut(s) 1473, 1641, 1987, 2213, 4300
BfuI GTATCC 1 cut(s) 3745
BglI GCCNNNNNGGC 1 cut(s) 3069
BglII AGATCT 1 cut(s) 2663
Bme1390I CCNGG 6 cut(s) 786, 1056, 1446, 1501, 1896, 4405
Bme18I GGWCC 5 cut(s) 451, 1777, 3586, 3773, 4449
BmgT120I GGNCC 7 cut(s) 451, 635, 776, 1777, 3586, 3773, 4449
BmiI GGNNCC 7 cut(s) 1506, 2181, 3042, 3135, 3379, 3587, 4045
BmrFI CCNGG 6 cut(s) 786, 1056, 1446, 1501, 1896, 4405
BmtI GCTAGC 1 cut(s) 1149
BpiI GAAGAC 3 cut(s) 463, 2157, 3680
BpmI CTGGAG 1 cut(s) 3296
Bpu10I CCTNAGC 3 cut(s) 330, 447, 2013
BpuEI CTTGAG 9 cut(s) 1682, 2802, 2898, 3141, 3331, 3483, 3779, 4097, 4274
BpuMI CCSGG 1 cut(s) 1501
BsaAI YACGTR 1 cut(s) 4156
BsaBI GATNNNNATC 4 cut(s) 912, 1190, 1806, 4005
BsaI GGTCTC 2 cut(s) 440, 3015
BsaJI CCNNGG 7 cut(s) 656, 1445, 1500, 2247, 2578, 3468, 4050
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 4 cut(s) 1718, 1748, 3670, 3700
Bsc4I CCNNNNNNNGG 9 cut(s) 98, 785, 1247, 1445, 3311, 3595, 3869, 4087, 4187
Bse1I ACTGG 7 cut(s) 208, 519, 1508, 1738, 3279, 3435, 3513
Bse3DI GCAATG 4 cut(s) 825, 1751, 1974, 2807
Bse8I GATNNNNATC 4 cut(s) 912, 1190, 1806, 4005
BseAI TCCGGA 1 cut(s) 91
BseBI CCWGG 5 cut(s) 786, 1056, 1446, 1896, 4405
BseDI CCNNGG 7 cut(s) 656, 1445, 1500, 2247, 2578, 3468, 4050
BseJI GATNNNNATC 4 cut(s) 912, 1190, 1806, 4005
BseLI CCNNNNNNNGG 9 cut(s) 98, 785, 1247, 1445, 3311, 3595, 3869, 4087, 4187
BseMI GCAATG 4 cut(s) 825, 1751, 1974, 2807
BseNI ACTGG 7 cut(s) 208, 519, 1508, 1738, 3279, 3435, 3513
BseRI GAGGAG 3 cut(s) 77, 250, 1739
BseYI CCCAGC 1 cut(s) 1992
Bsh1236I CGCG 2 cut(s) 2172, 4017
BshFI GGCC 8 cut(s) 162, 180, 637, 778, 1579, 2020, 2314, 4403
BshNI GGYRCC 2 cut(s) 2179, 4043
BsiHKAI GWGCWC 1 cut(s) 1416
BsiSI CCGG 2 cut(s) 92, 1500
BslFI GGGAC 3 cut(s) 1442, 1576, 3599
BslI CCNNNNNNNGG 9 cut(s) 98, 785, 1247, 1445, 3311, 3595, 3869, 4087, 4187
BsmAI GTCTC 8 cut(s) 145, 440, 502, 670, 2487, 3015, 3087, 3501
BsmBI CGTCTC 1 cut(s) 3501
BsmFI GGGAC 3 cut(s) 1442, 1576, 3599
BsmI GAATGC 5 cut(s) 1299, 1752, 1823, 1840, 4429
BsnI GGCC 8 cut(s) 162, 180, 637, 778, 1579, 2020, 2314, 4403
Bso31I GGTCTC 2 cut(s) 440, 3015
Bsp1286I GDGCHC 1 cut(s) 1416
Bsp13I TCCGGA 1 cut(s) 91
Bsp1407I TGTACA 1 cut(s) 2804
BspANI GGCC 8 cut(s) 162, 180, 637, 778, 1579, 2020, 2314, 4403
BspEI TCCGGA 1 cut(s) 91
BspFNI CGCG 2 cut(s) 2172, 4017
BspHI TCATGA 7 cut(s) 727, 925, 2635, 2749, 2851, 3049, 4324
BspLI GGNNCC 7 cut(s) 1506, 2181, 3042, 3135, 3379, 3587, 4045
BspMAI CTGCAG 2 cut(s) 2217, 4304
BspOI GCTAGC 1 cut(s) 1149
BspPI GGATC 4 cut(s) 897, 1199, 3504, 3774
BspT107I GGYRCC 2 cut(s) 2179, 4043
BspTNI GGTCTC 2 cut(s) 440, 3015
BsrBI CCGCTC 1 cut(s) 3813
BsrDI GCAATG 4 cut(s) 825, 1751, 1974, 2807
BsrGI TGTACA 1 cut(s) 2804
BsrI ACTGG 7 cut(s) 208, 519, 1508, 1738, 3279, 3435, 3513
BssECI CCNNGG 7 cut(s) 656, 1445, 1500, 2247, 2578, 3468, 4050
BssT1I CCWWGG 2 cut(s) 2247, 2578
Bst2UI CCWGG 5 cut(s) 786, 1056, 1446, 1896, 4405
Bst6I CTCTTC 8 cut(s) 520, 2169, 2751, 3051, 3864, 3938, 4056, 4363
BstAUI TGTACA 1 cut(s) 2804
BstBAI YACGTR 1 cut(s) 4156
BstC8I GCNNGC 5 cut(s) 265, 1061, 1147, 1452, 1917
BstDSI CCRYGG 2 cut(s) 656, 3468
BstENI CCTNNNNNAGG 2 cut(s) 783, 1443
BstFNI CGCG 2 cut(s) 2172, 4017
BstHHI GCGC 2 cut(s) 392, 2172
BstMAI GTCTC 8 cut(s) 145, 440, 502, 670, 2487, 3015, 3087, 3501
BstMWI GCNNNNNNNGC 7 cut(s) 352, 815, 1016, 2711, 2777, 2879, 3069
BstNI CCWGG 5 cut(s) 786, 1056, 1446, 1896, 4405
BstNSI RCATGY 5 cut(s) 267, 700, 1919, 3839, 3986
BstSCI CCNGG 6 cut(s) 784, 1054, 1444, 1499, 1894, 4403
BstSFI CTRYAG 5 cut(s) 1473, 1641, 1987, 2213, 4300
BstUI CGCG 2 cut(s) 2172, 4017
BstV2I GAAGAC 3 cut(s) 463, 2157, 3680
BstX2I RGATCY 3 cut(s) 2663, 3509, 3766
BstXI CCANNNNNNTGG 1 cut(s) 3667
BstYI RGATCY 3 cut(s) 2663, 3509, 3766
BsuI GTATCC 1 cut(s) 3745
BsuRI GGCC 8 cut(s) 162, 180, 637, 778, 1579, 2020, 2314, 4403
BtgI CCRYGG 2 cut(s) 656, 3468
BtgZI GCGATG 1 cut(s) 3858
BtsI GCAGTG 3 cut(s) 127, 546, 2294
Cac8I GCNNGC 5 cut(s) 265, 1061, 1147, 1452, 1917
CaiI CAGNNNCTG 1 cut(s) 377
CciI TCATGA 7 cut(s) 727, 925, 2635, 2749, 2851, 3049, 4324
CfoI GCGC 2 cut(s) 392, 2172
Cfr13I GGNCC 7 cut(s) 451, 635, 776, 1777, 3586, 3773, 4449
Csp6I GTAC 9 cut(s) 1001, 1701, 2180, 2535, 2805, 3897, 3938, 4044, 4109
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CviQI GTAC 9 cut(s) 1001, 1701, 2180, 2535, 2805, 3897, 3938, 4044, 4109
DraIII CACNNNGTG 1 cut(s) 1774
DrdI GACNNNNNNGTC 1 cut(s) 1050
DseDI GACNNNNNNGTC 1 cut(s) 1050
EaeI YGGCCR 1 cut(s) 4401
Eam1104I CTCTTC 8 cut(s) 520, 2169, 2751, 3051, 3864, 3938, 4056, 4363
EarI CTCTTC 8 cut(s) 520, 2169, 2751, 3051, 3864, 3938, 4056, 4363
Eco130I CCWWGG 2 cut(s) 2247, 2578
Eco31I GGTCTC 2 cut(s) 440, 3015
Eco32I GATATC 1 cut(s) 3172
Eco47I GGWCC 5 cut(s) 451, 1777, 3586, 3773, 4449
Eco57I CTGAAG 7 cut(s) 1482, 1715, 1851, 1869, 1871, 3962, 4439
Eco72I CACGTG 1 cut(s) 4156
EcoNI CCTNNNNNAGG 2 cut(s) 783, 1443
EcoO109I RGGNCCY 2 cut(s) 3586, 4449
EcoRI GAATTC 3 cut(s) 227, 380, 3220
EcoRII CCWGG 5 cut(s) 784, 1054, 1444, 1894, 4403
EcoRV GATATC 1 cut(s) 3172
EcoT14I CCWWGG 2 cut(s) 2247, 2578
EcoT22I ATGCAT 5 cut(s) 1825, 1917, 1948, 3241, 3984
ErhI CCWWGG 2 cut(s) 2247, 2578
Esp3I CGTCTC 1 cut(s) 3501
FalI AAGNNNNNCTT 6 cut(s) 330, 362, 2730, 2762, 2832, 2864
FaqI GGGAC 3 cut(s) 1442, 1576, 3599
FauI CCCGC 1 cut(s) 74
FauNDI CATATG 1 cut(s) 3088
FbaI TGATCA 2 cut(s) 1765, 2161
FblI GTMKAC 1 cut(s) 735
GlaI GCGC 2 cut(s) 391, 2171
GsaI CCCAGC 1 cut(s) 1996
GsuI CTGGAG 1 cut(s) 3296
HaeIII GGCC 8 cut(s) 162, 180, 637, 778, 1579, 2020, 2314, 4403
HapII CCGG 2 cut(s) 92, 1500
HhaI GCGC 2 cut(s) 392, 2172
Hin6I GCGC 2 cut(s) 390, 2170
HinP1I GCGC 2 cut(s) 390, 2170
HincII GTYRAC 1 cut(s) 4030
HindII GTYRAC 1 cut(s) 4030
HindIII AAGCTT 3 cut(s) 344, 611, 3662
HpaII CCGG 2 cut(s) 92, 1500
Hpy99I CGWCG 1 cut(s) 2205
HpyCH4IV ACGT 5 cut(s) 2856, 3934, 4105, 4155, 4266
HpyF10VI GCNNNNNNNGC 7 cut(s) 352, 815, 1016, 2711, 2777, 2879, 3069
HpySE526I ACGT 5 cut(s) 2856, 3934, 4105, 4155, 4266
HspAI GCGC 2 cut(s) 390, 2170
Kpn2I TCCGGA 1 cut(s) 91
KpnI GGTACC 2 cut(s) 2183, 4047
Ksp22I TGATCA 2 cut(s) 1765, 2161
LmnI GCTCC 6 cut(s) 64, 1132, 1504, 1726, 2705, 3040
MaeII ACGT 5 cut(s) 2856, 3934, 4105, 4155, 4266
MbiI CCGCTC 1 cut(s) 3813
MfeI CAATTG 3 cut(s) 1140, 1530, 3066
MflI RGATCY 3 cut(s) 2663, 3509, 3766
MhlI GDGCHC 1 cut(s) 1416
MlyI GAGTC 7 cut(s) 62, 567, 655, 2072, 3113, 3203, 3273
MmeI TCCRAC 5 cut(s) 775, 867, 2067, 2551, 3747
Mph1103I ATGCAT 5 cut(s) 1825, 1917, 1948, 3241, 3984
MroI TCCGGA 1 cut(s) 91
MroXI GAANNNNTTC 2 cut(s) 2109, 2964
MseI TTAA 8 cut(s) 38, 198, 488, 846, 944, 2223, 2418, 2988
MslI CAYNNNNRTG 3 cut(s) 1761, 1967, 2640
MspA1I CMGCKG 7 cut(s) 502, 998, 1992, 3699, 3870, 4188, 4417
MspI CCGG 2 cut(s) 92, 1500
MspR9I CCNGG 6 cut(s) 786, 1056, 1446, 1501, 1896, 4405
MunI CAATTG 3 cut(s) 1140, 1530, 3066
Mva1269I GAATGC 5 cut(s) 1299, 1752, 1823, 1840, 4429
MvaI CCWGG 5 cut(s) 786, 1056, 1446, 1896, 4405
MvnI CGCG 2 cut(s) 2172, 4017
MwoI GCNNNNNNNGC 7 cut(s) 352, 815, 1016, 2711, 2777, 2879, 3069
NciI CCSGG 1 cut(s) 1501
NdeI CATATG 1 cut(s) 3088
NheI GCTAGC 1 cut(s) 1145
NlaIV GGNNCC 7 cut(s) 1506, 2181, 3042, 3135, 3379, 3587, 4045
NmeAIII GCCGAG 1 cut(s) 2340
NmuCI GTSAC 4 cut(s) 651, 1421, 3176, 4441
NsiI ATGCAT 5 cut(s) 1825, 1917, 1948, 3241, 3984
NspI RCATGY 5 cut(s) 267, 700, 1919, 3839, 3986
PaeI GCATGC 2 cut(s) 267, 1919
PagI TCATGA 7 cut(s) 727, 925, 2635, 2749, 2851, 3049, 4324
PcsI WCGNNNNNNNCGW 1 cut(s) 2337
PctI GAATGC 5 cut(s) 1299, 1752, 1823, 1840, 4429
PdmI GAANNNNTTC 2 cut(s) 2109, 2964
PflFI GACNNNGTC 1 cut(s) 4447
PflMI CCANNNNNTGG 1 cut(s) 1247
PleI GAGTC 7 cut(s) 61, 566, 654, 2072, 3113, 3202, 3272
PmaCI CACGTG 1 cut(s) 4156
PmlI CACGTG 1 cut(s) 4156
PpsI GAGTC 7 cut(s) 61, 566, 654, 2072, 3113, 3202, 3272
Ppu21I YACGTR 1 cut(s) 4156
PpuMI RGGWCCY 2 cut(s) 3586, 4449
PsiI TTATAA 2 cut(s) 1287, 3129
Psp1406I AACGTT 1 cut(s) 4266
Psp5II RGGWCCY 2 cut(s) 3586, 4449
Psp6I CCWGG 5 cut(s) 784, 1054, 1444, 1894, 4403
PspCI CACGTG 1 cut(s) 4156
PspFI CCCAGC 1 cut(s) 1992
PspGI CCWGG 5 cut(s) 784, 1054, 1444, 1894, 4403
PspN4I GGNNCC 7 cut(s) 1506, 2181, 3042, 3135, 3379, 3587, 4045
PspPI GGNCC 7 cut(s) 451, 635, 776, 1777, 3586, 3773, 4449
PspPPI RGGWCCY 2 cut(s) 3586, 4449
PstI CTGCAG 2 cut(s) 2217, 4304
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 3 cut(s) 2663, 3509, 3766
PsyI GACNNNGTC 1 cut(s) 4447
PvuII CAGCTG 3 cut(s) 998, 1992, 4417
RsaI GTAC 9 cut(s) 1002, 1702, 2181, 2536, 2806, 3898, 3939, 4045, 4110
RsaNI GTAC 9 cut(s) 1001, 1701, 2180, 2535, 2805, 3897, 3938, 4044, 4109
RseI CAYNNNNRTG 3 cut(s) 1761, 1967, 2640
SaqAI TTAA 8 cut(s) 38, 198, 488, 846, 944, 2223, 2418, 2988
Sau96I GGNCC 7 cut(s) 451, 635, 776, 1777, 3586, 3773, 4449
SchI GAGTC 7 cut(s) 62, 567, 655, 2072, 3113, 3203, 3273
ScrFI CCNGG 6 cut(s) 786, 1056, 1446, 1501, 1896, 4405
SduI GDGCHC 1 cut(s) 1416
SfcI CTRYAG 5 cut(s) 1473, 1641, 1987, 2213, 4300
SinI GGWCC 5 cut(s) 451, 1777, 3586, 3773, 4449
SmiMI CAYNNNNRTG 3 cut(s) 1761, 1967, 2640
SmlI CTYRAG 9 cut(s) 1661, 2817, 2913, 3156, 3346, 3498, 3794, 4112, 4289
SmoI CTYRAG 9 cut(s) 1661, 2817, 2913, 3156, 3346, 3498, 3794, 4112, 4289
SpeI ACTAGT 1 cut(s) 2537
SphI GCATGC 2 cut(s) 267, 1919
SspI AATATT 4 cut(s) 286, 676, 1280, 2950
StyD4I CCNGG 6 cut(s) 784, 1054, 1444, 1499, 1894, 4403
StyI CCWWGG 2 cut(s) 2247, 2578
TaiI ACGT 5 cut(s) 2859, 3937, 4108, 4158, 4269
TaqI TCGA 4 cut(s) 231, 570, 1112, 2734
TaqII GACCGA 1 cut(s) 422
TatI WGTACW 5 cut(s) 1700, 2534, 2804, 3937, 4108
TauI GCSGC 3 cut(s) 505, 2020, 4191
Tru1I TTAA 8 cut(s) 38, 198, 488, 846, 944, 2223, 2418, 2988
Tru9I TTAA 8 cut(s) 38, 198, 488, 846, 944, 2223, 2418, 2988
TseFI GTSAC 4 cut(s) 651, 1421, 3176, 4441
Tsp45I GTSAC 4 cut(s) 651, 1421, 3176, 4441
TspGWI ACGGA 6 cut(s) 163, 416, 1261, 1580, 2218, 2972
Tth111I GACNNNGTC 1 cut(s) 4447
Van91I CCANNNNNTGG 1 cut(s) 1247
VpaK11BI GGWCC 5 cut(s) 451, 1777, 3586, 3773, 4449
XagI CCTNNNNNAGG 2 cut(s) 783, 1443
XbaI TCTAGA 1 cut(s) 3574
XceI RCATGY 5 cut(s) 267, 700, 1919, 3839, 3986
XmiI GTMKAC 1 cut(s) 735
XmnI GAANNNNTTC 2 cut(s) 2109, 2964
Zsp2I ATGCAT 5 cut(s) 1825, 1917, 1948, 3241, 3984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.