Rh5BG146600

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003630
Physical Location & Seq
Reverse (-)
15112448 .. 15116409
3962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG146600.1

Sequence Viewer

Length: 3732 bp
ATGGTTGTCATGATTAGTGAATTTGACAACCTTTTGCTAAGTACACCAATGGAGTCAGATGAGGAGCACAGGACTGTTCAAGGAATGTCTCCCGAATATGGTGCAATTTTCATGTCAAGCAGTTTTACAAAAGATGAATGTTTTAGACGAAGACTGTTTGGCCTTCCATCTGGACAAGGCCCATTTGTAAAGCAGATCAAAAGTGGAATGATTTTATTTCTCTTTGAATTTGAGAAGAGAGAACTCCATGGTGTATTCCAGGCATCCTCTGATGGTAGAATGAATATTGTTTCTAATGCATGGAGTAGATCAGGAAAGAAGTTTCCTGCTCAGGTGAGAGTCAACCCAATTTGGCATTGTCATCCACTTCCAGAATCTGAGTTTGGTGACGCTATCAAAGAAAACTATTTTTCAAAATGGAAATTCAATTTTGGTCTTTCCAAAGCTCAGGTTTGGAGGCTTCTAATGTTGTTTAATTTGCGAAAATTGAAAAGTCCACATCCTCAGAGAGCACTAGCTAGAAATACTGCTGCACAACCGGTGGACATTTATCGAGAACTTGATGATGGCAGATTATCAAGTGATAAAGTGGGTAATCTACATAAAGTGGACAATACGCTTCGGTCGATCATCCAGAGCAGGTATCTTGAACACCTGCCAGGCAAAGTTGAAAGAGATGATGCTAAGTTTGGGAATGGAGTTAATGTGGATTTTGAACACAGGGCATTGTCATCAACTGAGCATACTAGGTCAGGCCAAAATGGAAGATGTGATGGCAGCCTGGCAATGTGTGGAAGGGAAGGAAGTGTCAGTCACTTGGATGGTCCTTTTTATTCTGGAATGCCTCTTCAAGAAACATATTCTTTGGTTCAAGATAAAAAAACTTCTAGCCATCAGATGGAGCTGCCAATCACTGGCCACTCTTATGCAGCATCTCGTGGAGATGCAATTATTACGAGCATTCTTCCCTATGATCCTGATGATCCTAGTTTGAACCTTGCACATTCTCGATTAGTTGGAAATTATGATTCTGTTCAAGACTGTGATGGCCAGAATGGCATTCCCGCGGTGAGGAATGAAGTAAATCCCTCGTACACAGAGACAAATGGACTAAATCAAAGCCTAGAAGACATTTCTAAGGTTGATGTTCGCAGATCACTTTCAGCCATCAATATTACTCCTTTTCCAGGGGCAACATCTTCAGCAGAAATGGCTATTTACTATTCAAAGTCCTCTTTAGCTCCTGAATCTTTATCAGAATTTGGAAGTAATGGGAGGGACGGGCCTTCCTCTTATCCCCTTAGCCCCAGTAATTATCCTTCCTTTCTGGTTGAACGGAGGCATCCTGTAGCAATACAAAATAAACCAGTGCATGAAATAGGACCATTTACTGCAAATGTAACTTCATTAGAGGAGATACAGTGCCAGTCACCATGCCGCACTCATTCTGCTGACCTTGAGAGTGTGGACTACCACGACTGTAAATGTTCTGATAGAATGCTGTGTTCAGATCTTCTGGAAAACAGAAGTAGTGTGTTCTCTCGCCTGAGATTCAATAACCCTGGATGGTGTCTGGAAAACCTTGGACATCTTGGCCACGAGGAAGATGTTAAAGACACGTCATCAGTGGATGAAGTCATGGAAATGTTGAGCGAGAGTCACTACCCCTGGATGGAAAGTGAAAAATCTAAACTGCATAAGAGACGACAAGGTAATGTAGAGAAGTCTAGGAATTTGAAGCAGACTGCTGTGAAGTCTGAGTTGGAAATGATTCCAGAGAAGGCAAACACAGCTTCTGCTTCACCAACTGGAGATAAAGATGATCAAAGCAGTAAAAAGACACTGTTTGTGGACTTCAAGCGGCGGAGTGATTTGCGAAAAGTCGATAGCAATACTAATGGGGAAAGTGCTGGAAGCGAGGGATTGTTAGGTGGGCAGTGCAAGAGAAGGAAGTTAATCAGGCCTAACTTCAGTGAGAATGAGCCACGTGATAACAAAAGCTATAATAGAAGTCATTTTATGAATTCACCAGGATCAGCCCAAGAATGTCGTAGTTGTGAAGATGCTGGTGGCAAGAAAAGGAAGCTGGCTAAGCCAAATTTCAGAGAGAACGAGTCATCTCAGGAATCATCTGCGCAATGTTCTATTGGTGAAGATGCTGGTGGCAAGAGAAGGAAGCTGGCTAGGCCAAATTTCGGAGAAAATGAGTCATCTTTAATATCCTCGCCACAATGTTCTGTTGGTGAAGCTGCTGGTGGAAGTTGCGGGTCTTTTGTTGGAAACCAAGGTAAGTCGTTATTACAAGGTGCAGAATCTTCACACGATAATGAGAAGATCGATACTGAATGTGTTTCTAAATATGAAAGAGAAATTATAGTAGAAAGTTCTGGGGGTTCTCCCAAAATTGGAGATGATAGTGGAAAGGGGTGTTTGCATGCCTATCCTGGAAATTCTTGTCTGAACTTACAAGCATCAGTCGATGAATCTTCTTTTTGTGAAGATGCTAATTGTAAGAAGAAGTTAGTTGGGCCAGACTTCAGAGAGAATGAGCTATTCCAAGTGTCGCCACATGAATGTTCTAGTGGTGAATATGCTGGTGAAAATTATGAGGTATTGGTTGGAAGCCACGGTAACATTGATGCTCAAAGATGTCCAACATTCAAGATGGAAACTAAACTAGAAAGCTCTGGGGAGTCTCTCAAAATTGGAGATGAAAGTGGAAAGGATCCTGTGCATGATGTTGGCAGCCAAATTGCCATCGTCCCTTCCAAAGATGATATTCTTAATGCACAGGAAGACTTGGATCACAAGGACCCTATGGTGTTGTCTCGAGATGGTCATCAGCCCAGTCAGTCCACCATGCTAGAGTCAGCTTCTCAACTCAAGACAAATGGATTTTCTGAATTTGAAAGTGACAGAGGCAGTGAACTGGAGATGCTTACAAGGGTTGAATTTTCTCCAAACATAGCTGGCAATGGTGGCTCCAGGAAGCAGAACTCTGACTCACCAAGAGTATCTGAAAACATCTCTAATCGCGTGGATGCGGGTATGATAAATTATCAACACGGATCCAGTACTGAGCAGGGTCAGGCATCTTGCAACTCCAACCAGCATGATTCACTTAAACAGGAAGGGTTCGGGAATGACGTGCTGGAGGCTGTGGAGTTTGGTGATAATGTTGCTAGTGATTACACTTCAAGAGAAAGAGAAATAGACATTAAACTAGAAAGCTTTGTGGATTCTGTCAAAAGTGGAGATGAAAATGGAATGGAGAGCGTGCAGGTTGGTATTCTTAGTGCACTGGAAGACTCTATACTTACCAGGTTGTCTGAAGACTTTCCCGAGACCGATCAGATAACTGTGCTAGAGTCAGCTACGCAACCTAAGACGAATTCTGGAACAAGTAATGGATTTTCTGAAATCGAAGCTGACAGAGGCAGTGAACTGCAGGTGCTTCCAATAGTTGAACGTTGTCCAAACACAAACGCTTCCAATCGTGCAGATGCAAGTATGGTAAATAACCAACAAGGAGTCAATTCTGAGCAGTTGCAGACGTCTTGCAACTCCAACAATCACAGATCACATCAAGAGCATGGATCCAAACATAATGTGCAAGAGAGCATGGAGTTCGGCGATAATGAGGCAATTGATGACACTGTAAGAGAAAACAAGAGGCTATGGAGTATTTTAACAGCTAGATTGCAGCTTGTTAAGAGGGCACAACAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

1243

Amino Acids

137.25

Weight (kDa)

5.3

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 34 - 158 5.1e-48 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 663, 3439
AatII GACGTC 1 cut(s) 3557
Acc16I TGCGCA 1 cut(s) 2136
Acc36I ACCTGC 4 cut(s) 630, 663, 3271, 3439
AccB7I CCANNNNNTGG 2 cut(s) 898, 914
AccII CGCG 2 cut(s) 1067, 3036
AciI CCGC 7 cut(s) 1065, 1067, 1438, 1861, 1864, 2265, 3044
AclI AACGTT 1 cut(s) 3469
AcoI YGGCCR 3 cut(s) 916, 1048, 1594
AcuI CTGAAG 4 cut(s) 1185, 1954, 2521, 3351
AcvI CACGTG 1 cut(s) 1988
AcyI GRCGYC 1 cut(s) 3554
AfaI GTAC 3 cut(s) 43, 1094, 3076
AfiI CCNNNNNNNGG 8 cut(s) 98, 898, 914, 1071, 1187, 1672, 2195, 2405
AflIII ACRYGT 1 cut(s) 1617
AgeI ACCGGT 1 cut(s) 538
AjiI CACGTC 2 cut(s) 1620, 3148
AjuI GAANNNNNNNTTGG 4 cut(s) 366, 398, 1745, 1777
Alw21I GWGCWC 3 cut(s) 69, 514, 3301
Alw26I GTCTC 6 cut(s) 93, 1094, 1696, 2700, 2832, 3338
Alw44I GTGCAC 1 cut(s) 3297
AlwNI CAGNNNCTG 2 cut(s) 377, 1796
Ama87I CYCGRG 2 cut(s) 2829, 3341
ApaLI GTGCAC 1 cut(s) 3297
ApeKI GCWGC 7 cut(s) 530, 777, 904, 929, 2249, 2745, 3703
AsiGI ACCGGT 1 cut(s) 538
Asp700I GAANNNNTTC 2 cut(s) 1770, 3336
AspLEI GCGC 1 cut(s) 2137
AspS9I GGNCC 6 cut(s) 179, 824, 1283, 1382, 2528, 2812
AvaI CYCGRG 2 cut(s) 2829, 3341
AvaII GGWCC 3 cut(s) 824, 1382, 2812
BaeGI GKGCMC 2 cut(s) 3301, 3721
BalI TGGCCA 3 cut(s) 918, 1050, 1596
BamHI GGATCC 3 cut(s) 2725, 3068, 3596
BarI GAAGNNNNNNTAC 4 cut(s) 1303, 1335, 3297, 3329
BauI CACGAG 2 cut(s) 936, 1598
BbrPI CACGTG 1 cut(s) 1988
BbsI GAAGAC 5 cut(s) 157, 1134, 2802, 3312, 3339
Bbv12I GWGCWC 3 cut(s) 69, 514, 3301
BbvI GCAGC 7 cut(s) 517, 789, 891, 941, 2236, 2757, 3715
BcgI CGANNNNNNTGC 4 cut(s) 83, 117, 3610, 3644
BclI TGATCA 1 cut(s) 1822
BcoDI GTCTC 6 cut(s) 93, 1094, 1696, 2700, 2832, 3338
BfmI CTRYAG 2 cut(s) 1347, 3446
BfuAI ACCTGC 4 cut(s) 630, 663, 3271, 3439
BglI GCCNNNNNGGC 1 cut(s) 1056
BglII AGATCT 1 cut(s) 1510
BisI GCNGC 9 cut(s) 531, 778, 905, 930, 1438, 1862, 2250, 2746, 3704
BlpI GCTNAGC 1 cut(s) 2091
BlsI GCNGC 9 cut(s) 532, 779, 906, 931, 1439, 1863, 2251, 2747, 3705
BmcAI AGTACT 1 cut(s) 3076
Bme18I GGWCC 3 cut(s) 824, 1382, 2812
BmeT110I CYCGRG 2 cut(s) 2829, 3341
BmgBI CACGTC 2 cut(s) 1620, 3148
BmgT120I GGNCC 6 cut(s) 179, 824, 1283, 1382, 2528, 2812
BmiI GGNNCC 5 cut(s) 2727, 2814, 2983, 3070, 3598
BmrI ACTGGG 2 cut(s) 1302, 2841
BmuI ACTGGG 2 cut(s) 1302, 2841
BpiI GAAGAC 5 cut(s) 157, 1134, 2802, 3312, 3339
BpmI CTGGAG 4 cut(s) 1830, 2951, 2968, 3173
Bpu10I CCTNAGC 3 cut(s) 330, 447, 1301
Bpu1102I GCTNAGC 1 cut(s) 2091
BpuEI CTTGAG 2 cut(s) 1478, 2867
Bsa29I ATCGAT 1 cut(s) 2337
BsaAI YACGTR 1 cut(s) 1988
BsaBI GATNNNNATC 1 cut(s) 2838
BsaHI GRCGYC 1 cut(s) 3554
BsaI GGTCTC 1 cut(s) 3338
BsaJI CCNNGG 8 cut(s) 247, 1065, 1187, 1561, 1582, 1667, 2284, 2626
BsaWI WCCGGW 1 cut(s) 538
BsaXI ACNNNNNCTCC 4 cut(s) 690, 720, 1406, 1436
Bsc4I CCNNNNNNNGG 8 cut(s) 98, 898, 914, 1071, 1187, 1672, 2195, 2405
Bse118I RCCGGY 1 cut(s) 538
Bse1I ACTGG 9 cut(s) 919, 1308, 1367, 1426, 1813, 2847, 2934, 3072, 3306
Bse3DI GCAATG 3 cut(s) 792, 2144, 2980
Bse8I GATNNNNATC 1 cut(s) 2838
BseCI ATCGAT 1 cut(s) 2337
BseDI CCNNGG 8 cut(s) 247, 1065, 1187, 1561, 1582, 1667, 2284, 2626
BseJI GATNNNNATC 1 cut(s) 2838
BseLI CCNNNNNNNGG 8 cut(s) 98, 898, 914, 1071, 1187, 1672, 2195, 2405
BseMI GCAATG 3 cut(s) 792, 2144, 2980
BseNI ACTGG 9 cut(s) 919, 1308, 1367, 1426, 1813, 2847, 2934, 3072, 3306
BseRI GAGGAG 2 cut(s) 77, 1427
BseSI GKGCMC 2 cut(s) 3301, 3721
BseXI GCAGC 7 cut(s) 517, 789, 891, 941, 2236, 2757, 3715
BsgI GTGCAG 4 cut(s) 516, 2328, 3299, 3519
Bsh1236I CGCG 2 cut(s) 1067, 3036
Bsh1285I CGRYCG 1 cut(s) 626
BshTI ACCGGT 1 cut(s) 538
BshVI ATCGAT 1 cut(s) 2337
BsiEI CGRYCG 1 cut(s) 626
BsiHKAI GWGCWC 3 cut(s) 69, 514, 3301
BsiHKCI CYCGRG 2 cut(s) 2829, 3341
BsiSI CCGG 1 cut(s) 539
BslFI GGGAC 2 cut(s) 1292, 2747
BslI CCNNNNNNNGG 8 cut(s) 98, 898, 914, 1071, 1187, 1672, 2195, 2405
BsmAI GTCTC 6 cut(s) 93, 1094, 1696, 2700, 2832, 3338
BsmBI CGTCTC 1 cut(s) 1696
BsmFI GGGAC 2 cut(s) 1292, 2747
BsmI GAATGC 4 cut(s) 846, 960, 1059, 1503
Bso31I GGTCTC 1 cut(s) 3338
BsoBI CYCGRG 2 cut(s) 2829, 3341
Bsp1286I GDGCHC 4 cut(s) 69, 514, 3301, 3721
Bsp1720I GCTNAGC 1 cut(s) 2091
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 7 cut(s) 1065, 1067, 1438, 1861, 1864, 2265, 3044
BspDI ATCGAT 1 cut(s) 2337
BspFNI CGCG 2 cut(s) 1067, 3036
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 5 cut(s) 2727, 2814, 2983, 3070, 3598
BspMAI CTGCAG 1 cut(s) 3450
BspMI ACCTGC 4 cut(s) 630, 663, 3271, 3439
BspTNI GGTCTC 1 cut(s) 3338
BsrDI GCAATG 3 cut(s) 792, 2144, 2980
BsrFI RCCGGY 1 cut(s) 538
BsrI ACTGG 9 cut(s) 919, 1308, 1367, 1426, 1813, 2847, 2934, 3072, 3306
BssAI RCCGGY 1 cut(s) 538
BssECI CCNNGG 8 cut(s) 247, 1065, 1187, 1561, 1582, 1667, 2284, 2626
BssNI GRCGYC 1 cut(s) 3554
BssSI CACGAG 2 cut(s) 936, 1598
BssT1I CCWWGG 3 cut(s) 247, 1582, 2284
Bst2BI CACGAG 2 cut(s) 936, 1598
Bst4CI ACNGT 9 cut(s) 76, 156, 1043, 1422, 1481, 1845, 2630, 3361, 3658
Bst6I CTCTTC 2 cut(s) 230, 852
BstACI GRCGYC 1 cut(s) 3554
BstBAI YACGTR 1 cut(s) 1988
BstC8I GCNNGC 5 cut(s) 2088, 2181, 2436, 2971, 3278
BstDSI CCRYGG 3 cut(s) 247, 1065, 2626
BstENI CCTNNNNNAGG 1 cut(s) 1185
BstFNI CGCG 2 cut(s) 1067, 3036
BstHHI GCGC 1 cut(s) 2137
BstMAI GTCTC 6 cut(s) 93, 1094, 1696, 2700, 2832, 3338
BstMCI CGRYCG 1 cut(s) 626
BstMWI GCNNNNNNNGC 6 cut(s) 1056, 1211, 1790, 2092, 2185, 2979
BstNSI RCATGY 1 cut(s) 2438
BstSFI CTRYAG 2 cut(s) 1347, 3446
BstSLI GKGCMC 2 cut(s) 3301, 3721
BstUI CGCG 2 cut(s) 1067, 3036
BstV1I GCAGC 7 cut(s) 517, 789, 891, 941, 2236, 2757, 3715
BstV2I GAAGAC 5 cut(s) 157, 1134, 2802, 3312, 3339
BstX2I RGATCY 4 cut(s) 1510, 2725, 3068, 3596
BstYI RGATCY 4 cut(s) 1510, 2725, 3068, 3596
Bsu15I ATCGAT 1 cut(s) 2337
BsuTUI ATCGAT 1 cut(s) 2337
BtgI CCRYGG 3 cut(s) 247, 1065, 2626
BtrI CACGTC 2 cut(s) 1620, 3148
BtsI GCAGTG 3 cut(s) 1943, 2929, 3445
BveI ACCTGC 4 cut(s) 630, 663, 3271, 3439
Cac8I GCNNGC 5 cut(s) 2088, 2181, 2436, 2971, 3278
CaiI CAGNNNCTG 2 cut(s) 377, 1796
CciI TCATGA 1 cut(s) 9
CfoI GCGC 1 cut(s) 2137
Cfr10I RCCGGY 1 cut(s) 538
Cfr13I GGNCC 6 cut(s) 179, 824, 1283, 1382, 2528, 2812
Cfr42I CCGCGG 1 cut(s) 1068
ClaI ATCGAT 1 cut(s) 2337
CseI GACGC 1 cut(s) 398
CsiI ACCWGGT 1 cut(s) 3320
Csp6I GTAC 3 cut(s) 42, 1093, 3075
CspAI ACCGGT 1 cut(s) 538
CviQI GTAC 3 cut(s) 42, 1093, 3075
EaeI YGGCCR 3 cut(s) 916, 1048, 1594
Eam1104I CTCTTC 2 cut(s) 230, 852
EarI CTCTTC 2 cut(s) 230, 852
EciI GGCGGA 1 cut(s) 1879
Eco130I CCWWGG 3 cut(s) 247, 1582, 2284
Eco147I AGGCCT 1 cut(s) 1963
Eco31I GGTCTC 1 cut(s) 3338
Eco47I GGWCC 3 cut(s) 824, 1382, 2812
Eco57I CTGAAG 4 cut(s) 1185, 1954, 2521, 3351
Eco72I CACGTG 1 cut(s) 1988
Eco88I CYCGRG 2 cut(s) 2829, 3341
EcoNI CCTNNNNNAGG 1 cut(s) 1185
EcoO109I RGGNCCY 1 cut(s) 2812
EcoRI GAATTC 2 cut(s) 2023, 3391
EcoT14I CCWWGG 3 cut(s) 247, 1582, 2284
EcoT22I ATGCAT 1 cut(s) 301
ErhI CCWWGG 3 cut(s) 247, 1582, 2284
Esp3I CGTCTC 1 cut(s) 1696
FaqI GGGAC 2 cut(s) 1292, 2747
FauI CCCGC 3 cut(s) 1072, 2258, 3037
FbaI TGATCA 1 cut(s) 1822
Fnu4HI GCNGC 9 cut(s) 531, 778, 905, 930, 1438, 1862, 2250, 2746, 3704
Fsp4HI GCNGC 9 cut(s) 531, 778, 905, 930, 1438, 1862, 2250, 2746, 3704
FspI TGCGCA 1 cut(s) 2136
GlaI GCGC 1 cut(s) 2136
GluI GCNGC 9 cut(s) 531, 778, 905, 930, 1438, 1862, 2250, 2746, 3704
GsuI CTGGAG 4 cut(s) 1830, 2951, 2968, 3173
HapII CCGG 1 cut(s) 539
HgaI GACGC 1 cut(s) 398
HhaI GCGC 1 cut(s) 2137
Hin1I GRCGYC 1 cut(s) 3554
Hin6I GCGC 1 cut(s) 2135
HinP1I GCGC 1 cut(s) 2135
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 3229
HpaII CCGG 1 cut(s) 539
HpyCH4III ACNGT 9 cut(s) 76, 156, 1043, 1422, 1481, 1845, 2630, 3361, 3658
HpyCH4IV ACGT 5 cut(s) 1619, 1987, 3147, 3469, 3554
HpyF10VI GCNNNNNNNGC 6 cut(s) 1056, 1211, 1790, 2092, 2185, 2979
HpySE526I ACGT 5 cut(s) 1619, 1987, 3147, 3469, 3554
Hsp92I GRCGYC 1 cut(s) 3554
HspAI GCGC 1 cut(s) 2135
Ksp22I TGATCA 1 cut(s) 1822
KspI CCGCGG 1 cut(s) 1068
LmnI GCTCC 4 cut(s) 64, 901, 1246, 2987
Lsp1109I GCAGC 7 cut(s) 517, 789, 891, 941, 2236, 2757, 3715
MabI ACCWGGT 1 cut(s) 3320
MaeII ACGT 5 cut(s) 1619, 1987, 3147, 3469, 3554
MaeIII GTNAC 7 cut(s) 386, 812, 1399, 1428, 1658, 2630, 2912
MfeI CAATTG 1 cut(s) 3645
MflI RGATCY 4 cut(s) 1510, 2725, 3068, 3596
MhlI GDGCHC 4 cut(s) 69, 514, 3301, 3721
MlsI TGGCCA 3 cut(s) 918, 1050, 1596
MluNI TGGCCA 3 cut(s) 918, 1050, 1596
MmeI TCCRAC 7 cut(s) 997, 1743, 2257, 2599, 2678, 3129, 3591
Mox20I TGGCCA 3 cut(s) 918, 1050, 1596
Mph1103I ATGCAT 1 cut(s) 301
MroXI GAANNNNTTC 2 cut(s) 1770, 3336
MscI TGGCCA 3 cut(s) 918, 1050, 1596
MslI CAYNNNNRTG 5 cut(s) 819, 924, 1643, 2325, 2572
Msp20I TGGCCA 3 cut(s) 918, 1050, 1596
MspA1I CMGCKG 1 cut(s) 1067
MspI CCGG 1 cut(s) 539
MunI CAATTG 1 cut(s) 3645
Mva1269I GAATGC 4 cut(s) 846, 960, 1059, 1503
MvnI CGCG 2 cut(s) 1067, 3036
MwoI GCNNNNNNNGC 6 cut(s) 1056, 1211, 1790, 2092, 2185, 2979
NcoI CCATGG 1 cut(s) 247
NlaIV GGNNCC 5 cut(s) 2727, 2814, 2983, 3070, 3598
NmuCI GTSAC 5 cut(s) 386, 812, 1428, 1658, 2912
NsbI TGCGCA 1 cut(s) 2136
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 1 cut(s) 2438
PaeI GCATGC 1 cut(s) 2438
PaeR7I CTCGAG 1 cut(s) 2829
PagI TCATGA 1 cut(s) 9
PaqCI CACCTGC 2 cut(s) 663, 3439
PceI AGGCCT 1 cut(s) 1963
PcsI WCGNNNNNNNCGW 2 cut(s) 623, 3144
PctI GAATGC 4 cut(s) 846, 960, 1059, 1503
PdmI GAANNNNTTC 2 cut(s) 1770, 3336
PflMI CCANNNNNTGG 2 cut(s) 898, 914
PfoI TCCNGGA 2 cut(s) 2443, 2984
PinAI ACCGGT 1 cut(s) 538
PkrI GCNGC 9 cut(s) 532, 779, 906, 931, 1439, 1863, 2251, 2747, 3705
PmaCI CACGTG 1 cut(s) 1988
PmlI CACGTG 1 cut(s) 1988
Ppu21I YACGTR 1 cut(s) 1988
PpuMI RGGWCCY 1 cut(s) 2812
Psp1406I AACGTT 1 cut(s) 3469
Psp5II RGGWCCY 1 cut(s) 2812
PspCI CACGTG 1 cut(s) 1988
PspN4I GGNNCC 5 cut(s) 2727, 2814, 2983, 3070, 3598
PspPI GGNCC 6 cut(s) 179, 824, 1283, 1382, 2528, 2812
PspPPI RGGWCCY 1 cut(s) 2812
PstI CTGCAG 1 cut(s) 3450
PstNI CAGNNNCTG 2 cut(s) 377, 1796
PsuI RGATCY 4 cut(s) 1510, 2725, 3068, 3596
RsaI GTAC 3 cut(s) 43, 1094, 3076
RsaNI GTAC 3 cut(s) 42, 1093, 3075
RseI CAYNNNNRTG 5 cut(s) 819, 924, 1643, 2325, 2572
SacII CCGCGG 1 cut(s) 1068
SatI GCNGC 9 cut(s) 531, 778, 905, 930, 1438, 1862, 2250, 2746, 3704
Sau96I GGNCC 6 cut(s) 179, 824, 1283, 1382, 2528, 2812
ScaI AGTACT 1 cut(s) 3076
SduI GDGCHC 4 cut(s) 69, 514, 3301, 3721
SexAI ACCWGGT 1 cut(s) 3320
SfcI CTRYAG 2 cut(s) 1347, 3446
Sfr274I CTCGAG 1 cut(s) 2829
Sfr303I CCGCGG 1 cut(s) 1068
SgrBI CCGCGG 1 cut(s) 1068
SinI GGWCC 3 cut(s) 824, 1382, 2812
SlaI CTCGAG 1 cut(s) 2829
SmiMI CAYNNNNRTG 5 cut(s) 819, 924, 1643, 2325, 2572
SmlI CTYRAG 3 cut(s) 1457, 2829, 2882
SmoI CTYRAG 3 cut(s) 1457, 2829, 2882
SphI GCATGC 1 cut(s) 2438
SseBI AGGCCT 1 cut(s) 1963
SsiI CCGC 7 cut(s) 1065, 1067, 1438, 1861, 1864, 2265, 3044
SspI AATATT 2 cut(s) 286, 1174
StuI AGGCCT 1 cut(s) 1963
StyI CCWWGG 3 cut(s) 247, 1582, 2284
TaaI ACNGT 9 cut(s) 76, 156, 1043, 1422, 1481, 1845, 2630, 3361, 3658
TaiI ACGT 5 cut(s) 1622, 1990, 3150, 3472, 3557
TaqI TCGA 8 cut(s) 553, 626, 1009, 1884, 2337, 2478, 2830, 3423
TaqII GACCGA 2 cut(s) 612, 3362
TatI WGTACW 2 cut(s) 41, 3074
TauI GCSGC 2 cut(s) 1440, 1864
TseFI GTSAC 5 cut(s) 386, 812, 1428, 1658, 2912
TseI GCWGC 7 cut(s) 530, 777, 904, 929, 2249, 2745, 3703
Tsp45I GTSAC 5 cut(s) 386, 812, 1428, 1658, 2912
TspGWI ACGGA 2 cut(s) 1351, 3081
Van91I CCANNNNNTGG 2 cut(s) 898, 914
VneI GTGCAC 1 cut(s) 3297
VpaK11BI GGWCC 3 cut(s) 824, 1382, 2812
XagI CCTNNNNNAGG 1 cut(s) 1185
XceI RCATGY 1 cut(s) 2438
XhoI CTCGAG 1 cut(s) 2829
XmnI GAANNNNTTC 2 cut(s) 1770, 3336
ZraI GACGTC 1 cut(s) 3555
ZrmI AGTACT 1 cut(s) 3076
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.