Rh5DG146100

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003630
Physical Location & Seq
Reverse (-)
14912152 .. 14917736
5585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG146100.1

Sequence Viewer

Length: 3747 bp
ATGGTTGTCATGATTAGTGAATTTGACAGCCTTTTGCTAAGTACACCAATGGAGTCAGATGAGGAGCACAGGACTGTTCAAGGAATGTCTCCCGAATATGGTGCAATTTTCATGTCAAGCAGTTTTACAAAAGATGAATGTTTTAGACGAAGACTGTTTGGCCTTCCATCTGGACAAGGCCCATTTGTAAAGCAGATCAAAAGTGGAATGATTTTATTTCTCTTTGAATTTGAGAAGAGAGAACTCCATGGTGTATTCCAGGCATCCTCTGATGGTAGAATGAATATTGTTCCTAATGCATGGAGTAGATCAGGAAAGAAGTTTCCTGCTCAGGTGAGAGTCAACCCAATTTGGCATTGTCATCCGCTTCCAGAATCTGAGTTTGGTGACGCAATCAAAGAAAACTATTTTTCAAAATGGAAATTCAATTTTGGTCTTTCCAAAGCTCAGGTTTGGAGGCTTCTAATGTTGTTTAATTTGCGAAAATTGAAAAGCCCACATCCTCAGAGAGCACTAGCTAGAAATACTGCTGCACAACCAGTGGACATTTATCGAGAACTTGATGATGGCAGATTATCAAGTGATAAAGTGGGTAATCTACATAAAGTGGACAATACGCTTCGGTCGATCATCCAGAGCAGGTATCTTGAACACCTGCCAGGCAAAGTTGAAAGGGATGATGCTAAGTTTGGGAATGGAGTTAATGTGGATTTTGAACACAGGGCATTGCCATCAACTGAGCATACTAGGTCAGGCCAAAATGGAAGATGTGATGGCAGCCTGGAAATGTGTGGAAGGGAAGGAAGTGTCAGTCACTTGGATGGTCCTTTTTATTCTGGAATGCCTCTTCAAGAAACATATTCTTTGGTTCAAGATAAAGAAACTTCTAGCCATCAGATGGAGCTGCCAATCACTGGCCACTCTTATGCAGCATCTCGTGGAGATGCAATTATTACGAGCATTCTTCCCTATGATCCTGATGATCCTAGATTGAACCTTGCACATTCTCGACTAGTTGGAAATTATGATTCTGTTCAAGACTGTGATGGCCAGAATGGCATTCCCACGGTGAGGAATGAAGTAAATCCCTCGTACACAGAGACAAATTCACTAAATCAAAGCCTAGAAGACATTTCTAAGGTTGATGTTCGCAGATCTCTTTCAGCCATCAATATTACTCCTTTTCCAGGGGCAACATCTTCAGCAGAAATGGCTATTTACTATTCAAAGTCCTCTTTAGCTCCTGAATCTTTATCAGAATTTGGAAGTAATGGGAGGGACGGGCCTTCCTCTTATCCCCTTAGCCCCAGTGATTATCCTTCCTTTCTGGTTGACCGCAGGCATCCTGTAGCAATACAAAATAAACCAGTGCATGAAATAGGACCATTTACTGCAAATGTAACTTCATTAGAGGAGACACAGTGCCAGTCACCATGCCGCACTCATTCTGCTGACCTTGACAGTGTGGACTACCACGACTGTAAATGTTCTGATCATAAAATTTCTGATAGAATGCTGTGTTCAGATCTTCTGGAAAACAGAAATAGTGTGTTCGCTCGCCTGAGATTCAATAACCCTGGATGGTGTCTGGAAAACCTTGGACATCTTGGCCGCGAGGAAGATGTTAAAGACACGTCATCAGTGGATGAAGTCATGGAAATGTTGAGCGAGAGTCACTACCCCTGGATGGAGAGTGAAAAATCTATACCGCACAAGAGACGACAAGGTAATGTAGAGAAGTCTAGGAATTTGAAGCAGACAGCTGTGAAGTCTGAGTTGGAAATGATTCCAGAGAAGGCAAACACAGCTTCTGCTTCACCAACTGGAGATAAAGATGATCAAAGCAGTAAAAAGACACCGTTTGTGGACTTCAAGCGGCGGAGTGATTTGCGAAAAGTCAATAGCGATACTAATGGGGAAAGTGCTGGAAGCGAGGGATTGTTAGGTGGGCAGTGCAAGAGAAGGAAGTTAATCAGGCCTAACTTCAGTGAGAATGAGCCACGTGATAACAAAAGCTATAATAGAAGTCATTTTATGAATTCACCAGGATCAGCCCAAGAATGCCGTAGTTGTGAAGATGCTGGTGGCAAGAAAAGGAAGCTGGCTAAGCCAAATTTCAGAGAGAACGAGTCATCTCAGGAATCATCTGCGCAATGTTCTATTGGTGAAGATGCTGGTGGCAAGAGAAGGAAGCTGGCTAGGCCAAATTTTAGAGAAAATGAGTCATCTTTAATATCCTCGCCACAATGTTCTGTTGGTGAAGATGCTGGTGGAAGTTGCGGGTCTTTTGTTGGAAGCCAAGGTAAGTTGTTATTACAAGGTGCAGAATCTTCACACGACAATGAGAAGATCGATACTGAATGTGTTTCTAAATATGAAACAGAAATTATAGTAGAAAGTTCTGGGGGTTCTCCCAAAATTGGAGATGATAGTGTAAAGGGGTGTTTGCATGCCTATCCTGGAAATTCTTGTCTGAACTTACAAGCATCAGTCGATAAATCTTCTTTTTGTGAAGATGCTAATTGTAAGAAGGAGCTAGTTGGGCCTGATTTCAGAGAGAACGAGCTACTCCAAGTGTCGCCACATAAATGTTCTAGTGGTGAATATGCTGGTGAAAAGTATGAGGCATTGGTTGGAAGCCAAGGTAACATTGATGCTCAAATATTTCCAACATTTAAAATGGAAATTAAACAATTAAGCTCTGGGGAGTCTCTCAAAATTGGAGATGAAAGTGGAAAGGGTCCTGTGCATGATGTTGGCTGCCAAATTGCCATCGGCCCTTCCAAAGATGATATTCTTAATGCACAGGAAGACTTGGATCATAAGGACCCTATGGTGTTGTCTCGAGATGGTCATCAGCCCAGTCAGTCCACCGTGCTAAACTCAGCTTCGCAACCCAAGACAAATGGATTTTCCGAATTTGAAAGTGACAGAGGCAGTGAACTGGAGATGCTTCCAAGGGTTGAATTTTCTCCAAACGTAGTTGGCAATGGTGGCTCCAGTAAGCAGAACCCTGACTCACCTAGAGTAGCTGAAAACATCTCTAATCGCGTGGATGCGGGTATGATAAATTATCAACACGGATCCAGTACTCAGCAGGGTCAGGCATCTTGCAACTCCAACCAGCATGATTCACTTAAGCAGGAAGGGTTCGGGAATGACGTGCTGGAGGCTGTGGAGTTTGGTGATAATGTTGCAAGTGATAACACTTCAAGAGAAAGAGAAATAGACATCAAACTAGAAAGCTGTGTGGATTCTGTCAAAAGTGGAGATGAAAATGGAATGGAGAGCGTGCAGGTTGGTATTCTTAGTGCACTGGAAGACTCTATACTTACCAGGTTGTCTGAAGACTTTCCCGAGACCGATCAGATAACTGTGCTAGAGTCAGCTACGCAACCTAAGACGAATTCTGGAACAAGTAATGGATTTCCTGAAATCGAAGGTGACAGAGGCAGTGAACTGCAGGTGCTTCCAATAGTTGAACGTTGTCCAAATATAAACGCCTTTGATAGTGCAGATGCAAGTATGGTAAATAACCAACAAGGAGTCAATTCTGAGCAGTTGCAGACGTCTTGCAACTCCAACAATCACAGATCACTTCAAGAGCATGGATCCAAACATAATGTGCAAGAGAGCATGGAGTTCGGTGATAATGAGGCAATTGATGACACTGTAAGAGAAAACAAGAGGCTATGGAGAATTTTGACAGCTAGATTGCAGCTTGTTAAGAGGGCACAACAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

1248

Amino Acids

137.77

Weight (kDa)

5.26

Isoelectric Point (pI)

52.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 34 - 158 1.9e-48 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 663, 3454
AatII GACGTC 1 cut(s) 3572
Acc16I TGCGCA 1 cut(s) 2151
Acc36I ACCTGC 4 cut(s) 630, 663, 3286, 3454
AccB7I CCANNNNNTGG 2 cut(s) 898, 914
AccII CGCG 2 cut(s) 1614, 3051
AciI CCGC 9 cut(s) 365, 1336, 1438, 1612, 1709, 1876, 1879, 2280, 3059
AclI AACGTT 1 cut(s) 3484
AclWI GGATC 8 cut(s) 968, 977, 2056, 2826, 3078, 3091, 3606, 3619
AcoI YGGCCR 3 cut(s) 916, 1048, 1609
AcuI CTGAAG 3 cut(s) 1185, 1969, 3366
AcvI CACGTG 1 cut(s) 2003
AcyI GRCGYC 1 cut(s) 3569
AfaI GTAC 3 cut(s) 43, 1094, 3091
AfiI CCNNNNNNNGG 7 cut(s) 98, 898, 914, 1071, 1187, 1687, 2420
AflII CTTAAG 1 cut(s) 3137
AflIII ACRYGT 1 cut(s) 1632
AhlI ACTAGT 1 cut(s) 1012
AjiI CACGTC 2 cut(s) 1635, 3163
AjnI CCWGG 9 cut(s) 258, 658, 780, 1186, 1576, 1682, 2044, 2458, 3335
AjuI GAANNNNNNNTTGG 4 cut(s) 366, 398, 1760, 1792
Alw21I GWGCWC 3 cut(s) 69, 514, 3316
Alw26I GTCTC 7 cut(s) 93, 1094, 1409, 1711, 2715, 2847, 3353
Alw44I GTGCAC 1 cut(s) 3312
AlwI GGATC 8 cut(s) 968, 977, 2056, 2826, 3078, 3091, 3606, 3619
AlwNI CAGNNNCTG 2 cut(s) 377, 1811
Ama87I CYCGRG 2 cut(s) 2844, 3356
ApaLI GTGCAC 1 cut(s) 3312
ApeKI GCWGC 6 cut(s) 530, 777, 904, 929, 2760, 3718
Asp700I GAANNNNTTC 2 cut(s) 1785, 3351
AspLEI GCGC 1 cut(s) 2152
AspS9I GGNCC 8 cut(s) 179, 824, 1283, 1382, 2543, 2741, 2777, 2827
AvaI CYCGRG 2 cut(s) 2844, 3356
AvaII GGWCC 4 cut(s) 824, 1382, 2741, 2827
BaeGI GKGCMC 2 cut(s) 3316, 3736
BalI TGGCCA 2 cut(s) 918, 1050
BamHI GGATCC 2 cut(s) 3083, 3611
BarI GAAGNNNNNNTAC 2 cut(s) 3312, 3344
BauI CACGAG 1 cut(s) 936
BbrPI CACGTG 1 cut(s) 2003
BbsI GAAGAC 5 cut(s) 157, 1134, 2817, 3327, 3354
Bbv12I GWGCWC 3 cut(s) 69, 514, 3316
BbvI GCAGC 6 cut(s) 517, 789, 891, 941, 2747, 3730
BceAI ACGGC 1 cut(s) 2049
BcgI CGANNNNNNTGC 4 cut(s) 83, 117, 3625, 3659
BciT130I CCWGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3337
BclI TGATCA 2 cut(s) 1492, 1837
BcoDI GTCTC 7 cut(s) 93, 1094, 1409, 1711, 2715, 2847, 3353
BcuI ACTAGT 1 cut(s) 1012
BfmI CTRYAG 2 cut(s) 1347, 3461
BfrI CTTAAG 1 cut(s) 3137
BfuAI ACCTGC 4 cut(s) 630, 663, 3286, 3454
BglI GCCNNNNNGGC 1 cut(s) 1056
BglII AGATCT 2 cut(s) 1154, 1525
BisI GCNGC 9 cut(s) 531, 778, 905, 930, 1438, 1612, 1877, 2761, 3719
BlpI GCTNAGC 1 cut(s) 2106
BlsI GCNGC 9 cut(s) 532, 779, 906, 931, 1439, 1613, 1878, 2762, 3720
BmcAI AGTACT 1 cut(s) 3091
Bme1390I CCNGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3337
Bme18I GGWCC 4 cut(s) 824, 1382, 2741, 2827
BmeT110I CYCGRG 2 cut(s) 2844, 3356
BmgBI CACGTC 2 cut(s) 1635, 3163
BmgT120I GGNCC 8 cut(s) 179, 824, 1283, 1382, 2543, 2741, 2777, 2827
BmiI GGNNCC 5 cut(s) 2742, 2829, 2998, 3085, 3613
BmrFI CCNGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3337
BmrI ACTGGG 2 cut(s) 1302, 2856
BmuI ACTGGG 2 cut(s) 1302, 2856
BpiI GAAGAC 5 cut(s) 157, 1134, 2817, 3327, 3354
BpmI CTGGAG 4 cut(s) 1845, 2966, 2983, 3188
Bpu10I CCTNAGC 3 cut(s) 330, 447, 1301
Bpu1102I GCTNAGC 1 cut(s) 2106
Bsa29I ATCGAT 1 cut(s) 2352
BsaAI YACGTR 1 cut(s) 2003
BsaBI GATNNNNATC 1 cut(s) 2853
BsaHI GRCGYC 1 cut(s) 3569
BsaI GGTCTC 1 cut(s) 3353
BsaJI CCNNGG 9 cut(s) 247, 1065, 1187, 1576, 1597, 1682, 2299, 2641, 2957
BsaXI ACNNNNNCTCC 4 cut(s) 690, 720, 1406, 1436
Bsc4I CCNNNNNNNGG 7 cut(s) 98, 898, 914, 1071, 1187, 1687, 2420
Bse3DI GCAATG 3 cut(s) 725, 2159, 2995
Bse8I GATNNNNATC 1 cut(s) 2853
BseBI CCWGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3337
BseCI ATCGAT 1 cut(s) 2352
BseDI CCNNGG 9 cut(s) 247, 1065, 1187, 1576, 1597, 1682, 2299, 2641, 2957
BseJI GATNNNNATC 1 cut(s) 2853
BseLI CCNNNNNNNGG 7 cut(s) 98, 898, 914, 1071, 1187, 1687, 2420
BseMI GCAATG 3 cut(s) 725, 2159, 2995
BseRI GAGGAG 2 cut(s) 77, 1427
BseSI GKGCMC 2 cut(s) 3316, 3736
BseXI GCAGC 6 cut(s) 517, 789, 891, 941, 2747, 3730
BsgI GTGCAG 4 cut(s) 516, 2343, 3314, 3534
Bsh1236I CGCG 2 cut(s) 1614, 3051
Bsh1285I CGRYCG 1 cut(s) 626
BshVI ATCGAT 1 cut(s) 2352
BsiEI CGRYCG 1 cut(s) 626
BsiHKAI GWGCWC 3 cut(s) 69, 514, 3316
BsiHKCI CYCGRG 2 cut(s) 2844, 3356
BslFI GGGAC 1 cut(s) 1292
BslI CCNNNNNNNGG 7 cut(s) 98, 898, 914, 1071, 1187, 1687, 2420
BsmAI GTCTC 7 cut(s) 93, 1094, 1409, 1711, 2715, 2847, 3353
BsmBI CGTCTC 1 cut(s) 1711
BsmFI GGGAC 1 cut(s) 1292
BsmI GAATGC 5 cut(s) 846, 960, 1059, 1518, 2066
Bso31I GGTCTC 1 cut(s) 3353
BsoBI CYCGRG 2 cut(s) 2844, 3356
Bsp1286I GDGCHC 4 cut(s) 69, 514, 3316, 3736
Bsp1720I GCTNAGC 1 cut(s) 2106
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 9 cut(s) 365, 1336, 1438, 1612, 1709, 1876, 1879, 2280, 3059
BspDI ATCGAT 1 cut(s) 2352
BspFNI CGCG 2 cut(s) 1614, 3051
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 5 cut(s) 2742, 2829, 2998, 3085, 3613
BspMAI CTGCAG 1 cut(s) 3465
BspMI ACCTGC 4 cut(s) 630, 663, 3286, 3454
BspPI GGATC 8 cut(s) 968, 977, 2056, 2826, 3078, 3091, 3606, 3619
BspTI CTTAAG 1 cut(s) 3137
BspTNI GGTCTC 1 cut(s) 3353
BsrDI GCAATG 3 cut(s) 725, 2159, 2995
BssECI CCNNGG 9 cut(s) 247, 1065, 1187, 1576, 1597, 1682, 2299, 2641, 2957
BssNI GRCGYC 1 cut(s) 3569
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 5 cut(s) 247, 1597, 2299, 2641, 2957
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3337
Bst6I CTCTTC 2 cut(s) 230, 852
BstACI GRCGYC 1 cut(s) 3569
BstAFI CTTAAG 1 cut(s) 3137
BstBAI YACGTR 1 cut(s) 2003
BstC8I GCNNGC 6 cut(s) 1340, 1558, 2103, 2196, 2451, 3293
BstDSI CCRYGG 2 cut(s) 247, 1065
BstENI CCTNNNNNAGG 1 cut(s) 1185
BstFNI CGCG 2 cut(s) 1614, 3051
BstHHI GCGC 1 cut(s) 2152
BstMAI GTCTC 7 cut(s) 93, 1094, 1409, 1711, 2715, 2847, 3353
BstMCI CGRYCG 1 cut(s) 626
BstMWI GCNNNNNNNGC 7 cut(s) 1056, 1211, 1805, 2107, 2200, 2542, 2994
BstNI CCWGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3337
BstNSI RCATGY 1 cut(s) 2453
BstSCI CCNGG 9 cut(s) 258, 658, 780, 1186, 1576, 1682, 2044, 2458, 3335
BstSFI CTRYAG 2 cut(s) 1347, 3461
BstSLI GKGCMC 2 cut(s) 3316, 3736
BstUI CGCG 2 cut(s) 1614, 3051
BstV1I GCAGC 6 cut(s) 517, 789, 891, 941, 2747, 3730
BstV2I GAAGAC 5 cut(s) 157, 1134, 2817, 3327, 3354
BstX2I RGATCY 4 cut(s) 1154, 1525, 3083, 3611
BstYI RGATCY 4 cut(s) 1154, 1525, 3083, 3611
Bsu15I ATCGAT 1 cut(s) 2352
BsuTUI ATCGAT 1 cut(s) 2352
BtgI CCRYGG 2 cut(s) 247, 1065
BtrI CACGTC 2 cut(s) 1635, 3163
BtsI GCAGTG 3 cut(s) 1958, 2944, 3460
BveI ACCTGC 4 cut(s) 630, 663, 3286, 3454
Cac8I GCNNGC 6 cut(s) 1340, 1558, 2103, 2196, 2451, 3293
CaiI CAGNNNCTG 2 cut(s) 377, 1811
CciI TCATGA 1 cut(s) 9
CfoI GCGC 1 cut(s) 2152
Cfr13I GGNCC 8 cut(s) 179, 824, 1283, 1382, 2543, 2741, 2777, 2827
ClaI ATCGAT 1 cut(s) 2352
CseI GACGC 1 cut(s) 398
CsiI ACCWGGT 1 cut(s) 3335
Csp6I GTAC 3 cut(s) 42, 1093, 3090
CviQI GTAC 3 cut(s) 42, 1093, 3090
DraI TTTAAA 1 cut(s) 2677
EaeI YGGCCR 3 cut(s) 916, 1048, 1609
Eam1104I CTCTTC 2 cut(s) 230, 852
EarI CTCTTC 2 cut(s) 230, 852
EciI GGCGGA 1 cut(s) 1894
Eco130I CCWWGG 5 cut(s) 247, 1597, 2299, 2641, 2957
Eco147I AGGCCT 1 cut(s) 1978
Eco31I GGTCTC 1 cut(s) 3353
Eco47I GGWCC 4 cut(s) 824, 1382, 2741, 2827
Eco57I CTGAAG 3 cut(s) 1185, 1969, 3366
Eco72I CACGTG 1 cut(s) 2003
Eco88I CYCGRG 2 cut(s) 2844, 3356
EcoNI CCTNNNNNAGG 1 cut(s) 1185
EcoO109I RGGNCCY 2 cut(s) 2741, 2827
EcoRI GAATTC 2 cut(s) 2038, 3406
EcoRII CCWGG 9 cut(s) 258, 658, 780, 1186, 1576, 1682, 2044, 2458, 3335
EcoT14I CCWWGG 5 cut(s) 247, 1597, 2299, 2641, 2957
EcoT22I ATGCAT 1 cut(s) 301
ErhI CCWWGG 5 cut(s) 247, 1597, 2299, 2641, 2957
Esp3I CGTCTC 1 cut(s) 1711
FaqI GGGAC 1 cut(s) 1292
FauI CCCGC 2 cut(s) 2273, 3052
FbaI TGATCA 2 cut(s) 1492, 1837
Fnu4HI GCNGC 9 cut(s) 531, 778, 905, 930, 1438, 1612, 1877, 2761, 3719
Fsp4HI GCNGC 9 cut(s) 531, 778, 905, 930, 1438, 1612, 1877, 2761, 3719
FspI TGCGCA 1 cut(s) 2151
GlaI GCGC 1 cut(s) 2151
GluI GCNGC 9 cut(s) 531, 778, 905, 930, 1438, 1612, 1877, 2761, 3719
GsuI CTGGAG 4 cut(s) 1845, 2966, 2983, 3188
HgaI GACGC 1 cut(s) 398
HhaI GCGC 1 cut(s) 2152
Hin1I GRCGYC 1 cut(s) 3569
Hin6I GCGC 1 cut(s) 2150
HinP1I GCGC 1 cut(s) 2150
HincII GTYRAC 2 cut(s) 343, 1333
HindII GTYRAC 2 cut(s) 343, 1333
HpyCH4IV ACGT 6 cut(s) 1634, 2002, 2979, 3162, 3484, 3569
HpyF10VI GCNNNNNNNGC 7 cut(s) 1056, 1211, 1805, 2107, 2200, 2542, 2994
HpySE526I ACGT 6 cut(s) 1634, 2002, 2979, 3162, 3484, 3569
Hsp92I GRCGYC 1 cut(s) 3569
HspAI GCGC 1 cut(s) 2150
Ksp22I TGATCA 2 cut(s) 1492, 1837
LmnI GCTCC 5 cut(s) 64, 901, 1246, 2533, 3002
Lsp1109I GCAGC 6 cut(s) 517, 789, 891, 941, 2747, 3730
MabI ACCWGGT 1 cut(s) 3335
MaeII ACGT 6 cut(s) 1634, 2002, 2979, 3162, 3484, 3569
MaeIII GTNAC 8 cut(s) 386, 812, 1399, 1428, 1673, 2645, 2927, 3443
MfeI CAATTG 1 cut(s) 3660
MflI RGATCY 4 cut(s) 1154, 1525, 3083, 3611
MhlI GDGCHC 4 cut(s) 69, 514, 3316, 3736
MlsI TGGCCA 2 cut(s) 918, 1050
MluNI TGGCCA 2 cut(s) 918, 1050
MmeI TCCRAC 7 cut(s) 997, 1758, 2272, 2614, 2693, 3144, 3606
Mox20I TGGCCA 2 cut(s) 918, 1050
Mph1103I ATGCAT 1 cut(s) 301
MroXI GAANNNNTTC 2 cut(s) 1785, 3351
MscI TGGCCA 2 cut(s) 918, 1050
MslI CAYNNNNRTG 5 cut(s) 819, 924, 1658, 2340, 2587
Msp20I TGGCCA 2 cut(s) 918, 1050
MspA1I CMGCKG 1 cut(s) 1763
MspCI CTTAAG 1 cut(s) 3137
MspR9I CCNGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3337
MunI CAATTG 1 cut(s) 3660
Mva1269I GAATGC 5 cut(s) 846, 960, 1059, 1518, 2066
MvaI CCWGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3337
MvnI CGCG 2 cut(s) 1614, 3051
MwoI GCNNNNNNNGC 7 cut(s) 1056, 1211, 1805, 2107, 2200, 2542, 2994
NcoI CCATGG 1 cut(s) 247
NlaIV GGNNCC 5 cut(s) 2742, 2829, 2998, 3085, 3613
NmuCI GTSAC 6 cut(s) 386, 812, 1428, 1673, 2927, 3443
NsbI TGCGCA 1 cut(s) 2151
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 1 cut(s) 2453
PaeI GCATGC 1 cut(s) 2453
PaeR7I CTCGAG 1 cut(s) 2844
PagI TCATGA 1 cut(s) 9
PaqCI CACCTGC 2 cut(s) 663, 3454
PceI AGGCCT 1 cut(s) 1978
PcsI WCGNNNNNNNCGW 2 cut(s) 623, 3159
PctI GAATGC 5 cut(s) 846, 960, 1059, 1518, 2066
PdmI GAANNNNTTC 2 cut(s) 1785, 3351
PfeI GAWTC 9 cut(s) 374, 1028, 1247, 1566, 1786, 2141, 2327, 3131, 3254
PflMI CCANNNNNTGG 2 cut(s) 898, 914
PfoI TCCNGGA 1 cut(s) 2458
PkrI GCNGC 9 cut(s) 532, 779, 906, 931, 1439, 1613, 1878, 2762, 3720
PmaCI CACGTG 1 cut(s) 2003
PmlI CACGTG 1 cut(s) 2003
Ppu21I YACGTR 1 cut(s) 2003
PpuMI RGGWCCY 2 cut(s) 2741, 2827
Psp1406I AACGTT 1 cut(s) 3484
Psp5II RGGWCCY 2 cut(s) 2741, 2827
Psp6I CCWGG 9 cut(s) 258, 658, 780, 1186, 1576, 1682, 2044, 2458, 3335
PspCI CACGTG 1 cut(s) 2003
PspGI CCWGG 9 cut(s) 258, 658, 780, 1186, 1576, 1682, 2044, 2458, 3335
PspN4I GGNNCC 5 cut(s) 2742, 2829, 2998, 3085, 3613
PspPI GGNCC 8 cut(s) 179, 824, 1283, 1382, 2543, 2741, 2777, 2827
PspPPI RGGWCCY 2 cut(s) 2741, 2827
PstI CTGCAG 1 cut(s) 3465
PstNI CAGNNNCTG 2 cut(s) 377, 1811
PsuI RGATCY 4 cut(s) 1154, 1525, 3083, 3611
PvuII CAGCTG 1 cut(s) 1763
RsaI GTAC 3 cut(s) 43, 1094, 3091
RsaNI GTAC 3 cut(s) 42, 1093, 3090
RseI CAYNNNNRTG 5 cut(s) 819, 924, 1658, 2340, 2587
SatI GCNGC 9 cut(s) 531, 778, 905, 930, 1438, 1612, 1877, 2761, 3719
Sau96I GGNCC 8 cut(s) 179, 824, 1283, 1382, 2543, 2741, 2777, 2827
ScaI AGTACT 1 cut(s) 3091
ScrFI CCNGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3337
SduI GDGCHC 4 cut(s) 69, 514, 3316, 3736
SexAI ACCWGGT 1 cut(s) 3335
SfcI CTRYAG 2 cut(s) 1347, 3461
Sfr274I CTCGAG 1 cut(s) 2844
SinI GGWCC 4 cut(s) 824, 1382, 2741, 2827
SlaI CTCGAG 1 cut(s) 2844
SmiMI CAYNNNNRTG 5 cut(s) 819, 924, 1658, 2340, 2587
SmlI CTYRAG 2 cut(s) 2844, 3137
SmoI CTYRAG 2 cut(s) 2844, 3137
SpeI ACTAGT 1 cut(s) 1012
SphI GCATGC 1 cut(s) 2453
SseBI AGGCCT 1 cut(s) 1978
SsiI CCGC 9 cut(s) 365, 1336, 1438, 1612, 1709, 1876, 1879, 2280, 3059
SspI AATATT 3 cut(s) 286, 1174, 2664
StuI AGGCCT 1 cut(s) 1978
StyD4I CCNGG 9 cut(s) 258, 658, 780, 1186, 1576, 1682, 2044, 2458, 3335
StyI CCWWGG 5 cut(s) 247, 1597, 2299, 2641, 2957
TaiI ACGT 6 cut(s) 1637, 2005, 2982, 3165, 3487, 3572
TaqI TCGA 7 cut(s) 553, 626, 1009, 2352, 2493, 2845, 3438
TaqII GACCGA 2 cut(s) 612, 3377
TatI WGTACW 2 cut(s) 41, 3089
TauI GCSGC 3 cut(s) 1440, 1614, 1879
TfiI GAWTC 9 cut(s) 374, 1028, 1247, 1566, 1786, 2141, 2327, 3131, 3254
TseFI GTSAC 6 cut(s) 386, 812, 1428, 1673, 2927, 3443
TseI GCWGC 6 cut(s) 530, 777, 904, 929, 2760, 3718
Tsp45I GTSAC 6 cut(s) 386, 812, 1428, 1673, 2927, 3443
TspGWI ACGGA 1 cut(s) 3096
Van91I CCANNNNNTGG 2 cut(s) 898, 914
Vha464I CTTAAG 1 cut(s) 3137
VneI GTGCAC 1 cut(s) 3312
VpaK11BI GGWCC 4 cut(s) 824, 1382, 2741, 2827
XagI CCTNNNNNAGG 1 cut(s) 1185
XceI RCATGY 1 cut(s) 2453
XhoI CTCGAG 1 cut(s) 2844
XmnI GAANNNNTTC 2 cut(s) 1785, 3351
ZraI GACGTC 1 cut(s) 3570
ZrmI AGTACT 1 cut(s) 3091
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.