Rorug05G0057200

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003630
Physical Location & Seq
Forward (+)
5028768 .. 5031605
2838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0057200.1

Sequence Viewer

Length: 2838 bp
ATGGCCGAGGCACTCATTGCCGTGGTTCTGGAACAATTGGTTACAGTTGCATTTGACCAAACAAAAGAAGCTGTGAAGCTGATTTTGAATGCTAAGAAAGAGGTCAAAAAATTCAGCGACAATCTAAAAGCAATTCAAGCTGTGCTCGAGGATGCTGAGAAGCAGCAAGTGGAGAAGGCTAGCGTGAGAAACTGGTTGGATGGGCTGGATGATGTTTCTTACGAGATGGTTGACGTGCTAGATGAGTGGAACACTGAAATTATGAAACAAGAGACAAATGGAGTTGCTACTGAGAAAAAGAAAACCTCCAAGAAGGTATGTTTCTCCATTCCCTCGAATTGCTTTTGTTTTGGTCTAGTCCATAAGGCAACTTATCTTCATAAAATAGCTACTGAGATGAAAGAGTTGAATGAAAAGCTAACTCTGATTACTGCTCAGAGAGAGAAGTTTGGGTTTCATCAATCCTCAACTCGTAGGAATGATGAACAGCTTAAGCAACAGCCGAAAACTTCATCATTTGTCGATATTTCTAAAATATTTGGCAGAGAAGAAGCAAAAGCTGATTTGTTAAGCAAGTTGTTGAGTGATAGTAGAGAAGAAGGGAAAAGGGTCCTTGTCATCCCTATAGTGGGTATGGGAGGGATGGGCAAAACAACTCTTGCGCAATTAGCCCACAACAATGAAGCTGTAAAAACCTATTTTGAAAAGAGAATATGGGTTTGTGTCTCAGATCCTTTTGATGGGGTGAAGATTGCCAAAGCCATTATTAGTGGTACTGGTAATGACACCCCAAAGTCAAATGAGTTGCAAGATGTCTTGGAGTGTATGTCTAGATGTATTGAGAAGAAAAAGTGCTTGATTTTTCTAGATGATGTGTGGACCGAAGACCAAAGAGAGTGGGAAAGTTTAAACTTACCTTTAATAATGGAAACTTGTGCTAAAGGTAGCAAAATAGTGGTGACCACACGAAAAGAGAAAGTTGCTCAGATAATGGGAGCAACCACTCAAATGATCCATTTGGAGAAGCTGAGTGATGAAAATTGTTTGGCATTGTTCAATAGCATTGCATATTTAGGGAGGGAAGAAGATAAGGCTAATGGATTTGGAGCGATAGGTGAGAAAATAGCAAAAAAGTGTAACGGCCTGCCACTTGCTGCAAAAACTTTAGGGACTCTCGTGCGGTATAAGAAGGAAATGGGAGATTGGCAGAAGGTCTTGGATAGTGAGATATGGTCTCTAAAAGTGGTTGAGCAACAAGTTTTTCAACCACTTTTATTAAGTTATTATGAGTTGGTCCCTGTAGTTAAGCGTTGCCTTTTATACTGTAGTACATTTCCAAAAGGTTATCGGTTTAAGAAACACAATTTGATTGAGTTGTGGATGTCACAGGATTATCTTAATGGGAAAGACTATAAAGAAAAGGAAGAAAAAGGTCAAAATGTTTTCGATTCCTTAGTAATGCTATCATTTTTTCAAGATGTTGTGAAAGAAGTAGATTTTGATGACAAGAGCATTACTATATTTTGCAAAATGCATGATATTGTGCATGAGTTTGTGCAATTCCTCAATAAGAATGAATGCTCTACAATGGAGGTTAAAGAAGTAAACGGAAGAAAAACGGTAGTAAATGAGGAGGTCCGTCATTTGACTGTATTATTTGCAGTCTATGATTCTCTATTTTTGAATTTTATTTCACCGGTCAATTGCAAAAAGTTACGCACTCTTGCAACTTTTGATTCAAGAATGACTAGCAGAGATATAAAAGGAATCTCAAAGCTGAGATGTCTGAGGACTTTAAATTTAAGCTGCAACTACGACATTGAAGAACTTCCAGAAGAGATTGGTGAATTGATACATTTGAGGTATCTCGACTTGTCTGACAATATTTATTTGAAGCAACTACCCGACAGTGTTGGAAATTTATACAATTTGCAAACCTTGTGTCTTACTAATTTAAGATATCTTGAAGCACTGCCTGGAAATATGGGGAACTTGATTAATTTAAGACACCTTCATACCAAGGGTTGTGATGGGCTGGAGTATTTGCCGAGAGGTATGGCGAGATTAACAAGTCTGCAGACACTAAATAGGTGTCCTGTTACTAATGATAGCGGCCCCGGTGGACGCTTGAAATTAGGAGATCTGGGAAGCTTCGACCAGCTTCAGGGTAGTCTCAACATTCATATCGATGGGAATTTGGAGGATACGAGTGAGGCGCAGAAGGCTCGCTTGTGGAATAAAAAGCTTCATAATTTGGAGCTAAGATTTTCAAGTTCGAGGCAGAGCCGAGTACAAGTCGTCGAGGCGTTACGGCCACAACAAGATCTGGAAACTCTAACGATTGATTGTTATGATGGCACCACGGCGCCCAGTTGGATGATATCCTTGCAGAGTTTGACATCCCTTTCTCTTAGGGAATGGCATGGATGCGAATTTTTGCCTCCTCTTGGGAAATTGCCGTTCCTTGAAGAACTAAGAGTATACGGAATGGGCAAAGTGAAGACGGTTGGAGATGAATTTTTAGGAATAACGGGCACTCAAACATCACCATCCTCTATTTTTTTCCCAAAATTGAAACAACTCACCTTAGAATGGTTGAGTGGGTGCCAACAGTGGGAAGGAGTTGGAGGGTGGACAGAAAACGGTGGTGATGAAATTACAATTATGCCATGCCTTGTTTCCCTCAGAATTGGGGGCTGCCGTAAGCTGATGAACCTGCCACTCTTCCTGCAAAAGACACCGGTCAGGAATGTTGTCATCACCAAATGTCGTCAGAGTATTATAATGCCTGTCTGGCCCGAGAACTGTGACGTCAACATTTCTGAAGAGATCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

945

Amino Acids

107.22

Weight (kDa)

6.39

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 101 4.4e-19 Rx N-terminal domain
NB-ARC PF00931 188 - 357 3.1e-31 NB-ARC domain
WHD_DRP PF23559 445 - 519 2e-18 Disease resistance protein Winged helix domain
LRR_14 PF23598 572 - 864 3.3e-32 Leucine-rich repeat region
LRR_8 PF13855 596 - 650 1e-05 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 712 - 830 8e-27 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2783
AatII GACGTC 1 cut(s) 2814
Acc16I TGCGCA 1 cut(s) 663
Acc36I ACCTGC 1 cut(s) 2724
AccB1I GGYRCC 3 cut(s) 2357, 2365, 2604
AccI GTMKAC 1 cut(s) 2481
AciI CCGC 2 cut(s) 1180, 2112
AclWI GGATC 2 cut(s) 725, 1006
AcoI YGGCCR 2 cut(s) 3, 2312
AcsI RAATTY 7 cut(s) 110, 1684, 1798, 1918, 2194, 2432, 2516
AcuI CTGAAG 1 cut(s) 2147
AcyI GRCGYC 2 cut(s) 2366, 2811
AfaI GTAC 3 cut(s) 775, 1330, 2292
AfiI CCNNNNNNNGG 7 cut(s) 628, 629, 740, 2164, 2447, 2592, 2614
AflII CTTAAG 1 cut(s) 491
AgeI ACCGGT 2 cut(s) 1696, 2740
AjiI CACGTC 1 cut(s) 235
AjnI CCWGG 1 cut(s) 1975
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 4 cut(s) 266, 730, 1239, 2177
AlwI GGATC 2 cut(s) 725, 1006
Ama87I CYCGRG 2 cut(s) 146, 2798
AoxI GGCC 5 cut(s) 3, 1141, 2113, 2312, 2795
ApeKI GCWGC 4 cut(s) 163, 1154, 1806, 2697
ApoI RAATTY 7 cut(s) 110, 1684, 1798, 1918, 2194, 2432, 2516
ArsI GACNNNNNNTTYG 2 cut(s) 1927, 1959
AseI ATTAAT 1 cut(s) 1998
AsiGI ACCGGT 2 cut(s) 1696, 2740
Asp700I GAANNNNTTC 1 cut(s) 1827
AspLEI GCGC 3 cut(s) 664, 2218, 2368
AspS9I GGNCC 6 cut(s) 610, 879, 1294, 1636, 2114, 2796
AsuC2I CCSGG 1 cut(s) 2118
AsuHPI GGTGA 9 cut(s) 757, 970, 1127, 1686, 1856, 2538, 2575, 2660, 2752
AsuNHI GCTAGC 1 cut(s) 179
AvaI CYCGRG 2 cut(s) 146, 2798
AvaII GGWCC 4 cut(s) 610, 879, 1294, 1636
BaeGI GKGCMC 1 cut(s) 2537
BanI GGYRCC 3 cut(s) 2357, 2365, 2604
BauI CACGAG 1 cut(s) 1175
BbsI GAAGAC 2 cut(s) 891, 2507
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 4 cut(s) 175, 1141, 1793, 2684
BccI CCATC 8 cut(s) 194, 220, 637, 734, 2024, 2183, 2348, 2557
BceAI ACGGC 6 cut(s) 5, 1156, 2327, 2379, 2443, 2685
BcgI CGANNNNNNTGC 2 cut(s) 2207, 2241
BciT130I CCWGG 1 cut(s) 1977
BciVI GTATCC 1 cut(s) 2197
BcnI CCSGG 1 cut(s) 2118
BcoDI GTCTC 4 cut(s) 266, 730, 1239, 2177
BfaI CTAG 6 cut(s) 180, 239, 356, 831, 866, 1749
BfmI CTRYAG 4 cut(s) 624, 1299, 1324, 2075
BfoI RGCGCY 1 cut(s) 2369
BfrI CTTAAG 1 cut(s) 491
BfuAI ACCTGC 1 cut(s) 2724
BfuI GTATCC 1 cut(s) 2197
BglI GCCNNNNNGGC 1 cut(s) 2794
BglII AGATCT 2 cut(s) 2140, 2323
BisI GCNGC 5 cut(s) 164, 1155, 1807, 2113, 2698
BlsI GCNGC 5 cut(s) 165, 1156, 1808, 2114, 2699
Bme1390I CCNGG 2 cut(s) 1977, 2118
Bme18I GGWCC 4 cut(s) 610, 879, 1294, 1636
BmeT110I CYCGRG 2 cut(s) 146, 2798
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 6 cut(s) 610, 879, 1294, 1636, 2114, 2796
BmiI GGNNCC 6 cut(s) 611, 1296, 2116, 2359, 2367, 2606
BmrFI CCNGG 2 cut(s) 1977, 2118
BmrI ACTGGG 1 cut(s) 2364
BmsI GCATC 2 cut(s) 142, 2417
BmtI GCTAGC 1 cut(s) 183
BmuI ACTGGG 1 cut(s) 2364
BoxI GACNNNNGTC 1 cut(s) 2741
BpiI GAAGAC 2 cut(s) 891, 2507
BpmI CTGGAG 1 cut(s) 2057
BpuMI CCSGG 1 cut(s) 2118
Bsa29I ATCGAT 1 cut(s) 2187
BsaHI GRCGYC 2 cut(s) 2366, 2811
BsaI GGTCTC 1 cut(s) 1239
BsaJI CCNNGG 5 cut(s) 6, 21, 2019, 2116, 2361
BsaWI WCCGGW 2 cut(s) 1696, 2740
BsaXI ACNNNNNCTCC 2 cut(s) 812, 842
Bsc4I CCNNNNNNNGG 7 cut(s) 628, 629, 740, 2164, 2447, 2592, 2614
Bse118I RCCGGY 2 cut(s) 1696, 2740
Bse1I ACTGG 3 cut(s) 197, 781, 2370
Bse3DI GCAATG 2 cut(s) 15, 1062
BseBI CCWGG 1 cut(s) 1977
BseCI ATCGAT 1 cut(s) 2187
BseDI CCNNGG 5 cut(s) 6, 21, 2019, 2116, 2361
BseLI CCNNNNNNNGG 7 cut(s) 628, 629, 740, 2164, 2447, 2592, 2614
BseMI GCAATG 2 cut(s) 15, 1062
BseNI ACTGG 3 cut(s) 197, 781, 2370
BseRI GAGGAG 2 cut(s) 1646, 2433
BseSI GKGCMC 1 cut(s) 2537
BseXI GCAGC 4 cut(s) 175, 1141, 1793, 2684
BshFI GGCC 5 cut(s) 5, 1143, 2115, 2314, 2797
BshNI GGYRCC 3 cut(s) 2357, 2365, 2604
BshTI ACCGGT 2 cut(s) 1696, 2740
BshVI ATCGAT 1 cut(s) 2187
BsiHKAI GWGCWC 1 cut(s) 147
BsiHKCI CYCGRG 2 cut(s) 146, 2798
BsiSI CCGG 3 cut(s) 1697, 2118, 2741
BslFI GGGAC 2 cut(s) 1183, 1280
BslI CCNNNNNNNGG 7 cut(s) 628, 629, 740, 2164, 2447, 2592, 2614
BsmAI GTCTC 4 cut(s) 266, 730, 1239, 2177
BsmFI GGGAC 2 cut(s) 1183, 1280
BsmI GAATGC 2 cut(s) 94, 1583
BsnI GGCC 5 cut(s) 5, 1143, 2115, 2314, 2797
Bso31I GGTCTC 1 cut(s) 1239
BsoBI CYCGRG 2 cut(s) 146, 2798
Bsp1286I GDGCHC 2 cut(s) 147, 2537
Bsp143I GATC 5 cut(s) 730, 1011, 2140, 2323, 2829
BspACI CCGC 2 cut(s) 1180, 2112
BspANI GGCC 5 cut(s) 5, 1143, 2115, 2314, 2797
BspDI ATCGAT 1 cut(s) 2187
BspLI GGNNCC 6 cut(s) 611, 1296, 2116, 2359, 2367, 2606
BspMAI CTGCAG 1 cut(s) 2079
BspMI ACCTGC 1 cut(s) 2724
BspOI GCTAGC 1 cut(s) 183
BspPI GGATC 2 cut(s) 725, 1006
BspT107I GGYRCC 3 cut(s) 2357, 2365, 2604
BspTI CTTAAG 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 1239
BsrDI GCAATG 2 cut(s) 15, 1062
BsrFI RCCGGY 2 cut(s) 1696, 2740
BsrI ACTGG 3 cut(s) 197, 781, 2370
BssAI RCCGGY 2 cut(s) 1696, 2740
BssECI CCNNGG 5 cut(s) 6, 21, 2019, 2116, 2361
BssMI GATC 5 cut(s) 730, 1011, 2140, 2323, 2829
BssNAI GTATAC 1 cut(s) 2482
BssNI GRCGYC 2 cut(s) 2366, 2811
BssSI CACGAG 1 cut(s) 1175
BssT1I CCWWGG 1 cut(s) 2019
Bst1107I GTATAC 1 cut(s) 2482
Bst2BI CACGAG 1 cut(s) 1175
Bst2UI CCWGG 1 cut(s) 1977
Bst4CI ACNGT 9 cut(s) 46, 1325, 1621, 1651, 1910, 2506, 2613, 2645, 2807
Bst6I CTCTTC 3 cut(s) 1830, 2729, 2820
BstACI GRCGYC 2 cut(s) 2366, 2811
BstAFI CTTAAG 1 cut(s) 491
BstAPI GCANNNNNTGC 1 cut(s) 17
BstC8I GCNNGC 3 cut(s) 181, 1145, 2227
BstDSI CCRYGG 2 cut(s) 21, 2361
BstEII GGTNACC 1 cut(s) 958
BstH2I RGCGCY 1 cut(s) 2369
BstHHI GCGC 3 cut(s) 664, 2218, 2368
BstKTI GATC 5 cut(s) 733, 1014, 2143, 2326, 2832
BstMAI GTCTC 4 cut(s) 266, 730, 1239, 2177
BstMBI GATC 5 cut(s) 730, 1011, 2140, 2323, 2829
BstMWI GCNNNNNNNGC 5 cut(s) 17, 137, 668, 2222, 2794
BstNI CCWGG 1 cut(s) 1977
BstPAI GACNNNNGTC 1 cut(s) 2741
BstPI GGTNACC 1 cut(s) 958
BstSCI CCNGG 2 cut(s) 1975, 2116
BstSFI CTRYAG 4 cut(s) 624, 1299, 1324, 2075
BstSLI GKGCMC 1 cut(s) 2537
BstV1I GCAGC 4 cut(s) 175, 1141, 1793, 2684
BstV2I GAAGAC 2 cut(s) 891, 2507
BstX2I RGATCY 3 cut(s) 730, 2140, 2323
BstYI RGATCY 3 cut(s) 730, 2140, 2323
BstZ17I GTATAC 1 cut(s) 2482
Bsu15I ATCGAT 1 cut(s) 2187
BsuI GTATCC 1 cut(s) 2197
BsuRI GGCC 5 cut(s) 5, 1143, 2115, 2314, 2797
BsuTUI ATCGAT 1 cut(s) 2187
BtgI CCRYGG 2 cut(s) 21, 2361
BtrI CACGTC 1 cut(s) 235
BtsI GCAGTG 1 cut(s) 1970
BtsIMutI CAGTG 4 cut(s) 252, 1915, 1970, 2618
BveI ACCTGC 1 cut(s) 2724
Cac8I GCNNGC 3 cut(s) 181, 1145, 2227
CfoI GCGC 3 cut(s) 664, 2218, 2368
Cfr10I RCCGGY 2 cut(s) 1696, 2740
Cfr13I GGNCC 6 cut(s) 610, 879, 1294, 1636, 2114, 2796
ClaI ATCGAT 1 cut(s) 2187
CseI GACGC 1 cut(s) 2133
Csp6I GTAC 3 cut(s) 774, 1329, 2291
CspAI ACCGGT 2 cut(s) 1696, 2740
CspCI CAANNNNNGTGG 2 cut(s) 878, 913
CviAII CATG 4 cut(s) 1535, 1547, 2423, 2670
CviQI GTAC 3 cut(s) 774, 1329, 2291
DinI GGCGCC 1 cut(s) 2367
DpnI GATC 5 cut(s) 732, 1013, 2142, 2325, 2831
DpnII GATC 5 cut(s) 730, 1011, 2140, 2323, 2829
DraI TTTAAA 2 cut(s) 909, 1797
EaeI YGGCCR 2 cut(s) 3, 2312
Eam1104I CTCTTC 3 cut(s) 1830, 2729, 2820
EarI CTCTTC 3 cut(s) 1830, 2729, 2820
Eco130I CCWWGG 1 cut(s) 2019
Eco31I GGTCTC 1 cut(s) 1239
Eco32I GATATC 2 cut(s) 1961, 2382
Eco47I GGWCC 4 cut(s) 610, 879, 1294, 1636
Eco57I CTGAAG 1 cut(s) 2147
Eco88I CYCGRG 2 cut(s) 146, 2798
Eco91I GGTNACC 1 cut(s) 958
EcoO109I RGGNCCY 1 cut(s) 610
EcoO65I GGTNACC 1 cut(s) 958
EcoRII CCWGG 1 cut(s) 1975
EcoRV GATATC 2 cut(s) 1961, 2382
EcoT14I CCWWGG 1 cut(s) 2019
EcoT22I ATGCAT 1 cut(s) 1536
EgeI GGCGCC 1 cut(s) 2367
EheI GGCGCC 1 cut(s) 2367
ErhI CCWWGG 1 cut(s) 2019
FaeI CATG 4 cut(s) 1538, 1550, 2426, 2673
FalI AAGNNNNNCTT 2 cut(s) 2213, 2245
FaqI GGGAC 2 cut(s) 1183, 1280
FatI CATG 4 cut(s) 1534, 1546, 2422, 2669
FblI GTMKAC 1 cut(s) 2481
Fnu4HI GCNGC 5 cut(s) 164, 1155, 1807, 2113, 2698
Fsp4HI GCNGC 5 cut(s) 164, 1155, 1807, 2113, 2698
FspBI CTAG 6 cut(s) 180, 239, 356, 831, 866, 1749
FspI TGCGCA 1 cut(s) 663
GlaI GCGC 3 cut(s) 663, 2217, 2367
GluI GCNGC 5 cut(s) 164, 1155, 1807, 2113, 2698
GsuI CTGGAG 1 cut(s) 2057
HaeII RGCGCY 1 cut(s) 2369
HaeIII GGCC 5 cut(s) 5, 1143, 2115, 2314, 2797
HapII CCGG 3 cut(s) 1697, 2118, 2741
HgaI GACGC 1 cut(s) 2133
HhaI GCGC 3 cut(s) 664, 2218, 2368
Hin1I GRCGYC 2 cut(s) 2366, 2811
Hin1II CATG 4 cut(s) 1538, 1550, 2426, 2673
Hin6I GCGC 3 cut(s) 662, 2216, 2366
HinP1I GCGC 3 cut(s) 662, 2216, 2366
HincII GTYRAC 2 cut(s) 232, 2815
HindII GTYRAC 2 cut(s) 232, 2815
HindIII AAGCTT 2 cut(s) 2149, 2243
HinfI GANTC 5 cut(s) 1171, 1448, 1670, 1736, 1767
HpaII CCGG 3 cut(s) 1697, 2118, 2741
HphI GGTGA 9 cut(s) 757, 970, 1127, 1686, 1856, 2538, 2575, 2660, 2752
Hpy166II GTNNAC 7 cut(s) 232, 879, 1606, 2123, 2482, 2634, 2815
Hpy188I TCNGA 9 cut(s) 426, 438, 730, 987, 1788, 1879, 2687, 2775, 2824
Hpy8I GTNNAC 7 cut(s) 232, 879, 1606, 2123, 2482, 2634, 2815
Hpy99I CGWCG 1 cut(s) 2303
HpyAV CCTTC 8 cut(s) 169, 307, 593, 1183, 1204, 2021, 2215, 2612
HpyCH4III ACNGT 9 cut(s) 46, 1325, 1621, 1651, 1910, 2506, 2613, 2645, 2807
HpyCH4IV ACGT 2 cut(s) 234, 2811
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 137, 668, 2222, 2794
HpySE526I ACGT 2 cut(s) 234, 2811
Hsp92I GRCGYC 2 cut(s) 2366, 2811
Hsp92II CATG 4 cut(s) 1538, 1550, 2426, 2673
HspAI GCGC 3 cut(s) 662, 2216, 2366
KasI GGCGCC 1 cut(s) 2365
Kzo9I GATC 5 cut(s) 730, 1011, 2140, 2323, 2829
LmnI GCTCC 3 cut(s) 995, 1106, 2257
Lsp1109I GCAGC 4 cut(s) 175, 1141, 1793, 2684
LweI GCATC 2 cut(s) 142, 2417
MaeI CTAG 6 cut(s) 180, 239, 356, 831, 866, 1749
MaeII ACGT 2 cut(s) 234, 2811
MaeIII GTNAC 8 cut(s) 40, 958, 1136, 1383, 1713, 2098, 2307, 2807
MalI GATC 5 cut(s) 732, 1013, 2142, 2325, 2831
MboI GATC 5 cut(s) 730, 1011, 2140, 2323, 2829
MfeI CAATTG 2 cut(s) 35, 1702
MflI RGATCY 3 cut(s) 730, 2140, 2323
MhlI GDGCHC 2 cut(s) 147, 2537
Mly113I GGCGCC 1 cut(s) 2366
MlyI GAGTC 1 cut(s) 1165
MmeI TCCRAC 5 cut(s) 177, 1894, 2354, 2488, 2605
Mph1103I ATGCAT 1 cut(s) 1536
MroXI GAANNNNTTC 1 cut(s) 1827
MslI CAYNNNNRTG 4 cut(s) 20, 678, 1007, 2187
MspCI CTTAAG 1 cut(s) 491
MspI CCGG 3 cut(s) 1697, 2118, 2741
MspR9I CCNGG 2 cut(s) 1977, 2118
MssI GTTTAAAC 1 cut(s) 909
MunI CAATTG 2 cut(s) 35, 1702
Mva1269I GAATGC 2 cut(s) 94, 1583
MvaI CCWGG 1 cut(s) 1977
MwoI GCNNNNNNNGC 5 cut(s) 17, 137, 668, 2222, 2794
NarI GGCGCC 1 cut(s) 2366
NciI CCSGG 1 cut(s) 2118
NdeII GATC 5 cut(s) 730, 1011, 2140, 2323, 2829
NheI GCTAGC 1 cut(s) 179
NlaIII CATG 4 cut(s) 1538, 1550, 2426, 2673
NlaIV GGNNCC 6 cut(s) 611, 1296, 2116, 2359, 2367, 2606
NmeAIII GCCGAG 3 cut(s) 31, 2073, 2312
NmuCI GTSAC 3 cut(s) 958, 1383, 2807
NsbI TGCGCA 1 cut(s) 663
NsiI ATGCAT 1 cut(s) 1536
PaeR7I CTCGAG 1 cut(s) 146
PctI GAATGC 2 cut(s) 94, 1583
PdmI GAANNNNTTC 1 cut(s) 1827
PfeI GAWTC 4 cut(s) 1448, 1670, 1736, 1767
PinAI ACCGGT 2 cut(s) 1696, 2740
PkrI GCNGC 5 cut(s) 165, 1156, 1808, 2114, 2699
PleI GAGTC 1 cut(s) 1165
PluTI GGCGCC 1 cut(s) 2369
PmeI GTTTAAAC 1 cut(s) 909
PpsI GAGTC 1 cut(s) 1165
PpuMI RGGWCCY 1 cut(s) 610
PshAI GACNNNNGTC 1 cut(s) 2741
PshBI ATTAAT 1 cut(s) 1998
PsiI TTATAA 1 cut(s) 2783
Psp5II RGGWCCY 1 cut(s) 610
Psp6I CCWGG 1 cut(s) 1975
PspEI GGTNACC 1 cut(s) 958
PspGI CCWGG 1 cut(s) 1975
PspN4I GGNNCC 6 cut(s) 611, 1296, 2116, 2359, 2367, 2606
PspPI GGNCC 6 cut(s) 610, 879, 1294, 1636, 2114, 2796
PspPPI RGGWCCY 1 cut(s) 610
PspXI VCTCGAGB 1 cut(s) 146
PstI CTGCAG 1 cut(s) 2079
PsuI RGATCY 3 cut(s) 730, 2140, 2323
RsaI GTAC 3 cut(s) 775, 1330, 2292
RsaNI GTAC 3 cut(s) 774, 1329, 2291
RseI CAYNNNNRTG 4 cut(s) 20, 678, 1007, 2187
SatI GCNGC 5 cut(s) 164, 1155, 1807, 2113, 2698
Sau3AI GATC 5 cut(s) 730, 1011, 2140, 2323, 2829
Sau96I GGNCC 6 cut(s) 610, 879, 1294, 1636, 2114, 2796
SchI GAGTC 1 cut(s) 1165
ScrFI CCNGG 2 cut(s) 1977, 2118
SduI GDGCHC 2 cut(s) 147, 2537
SfaNI GCATC 2 cut(s) 142, 2417
SfcI CTRYAG 4 cut(s) 624, 1299, 1324, 2075
SfoI GGCGCC 1 cut(s) 2367
Sfr274I CTCGAG 1 cut(s) 146
SinI GGWCC 4 cut(s) 610, 879, 1294, 1636
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 4 cut(s) 20, 678, 1007, 2187
SmlI CTYRAG 2 cut(s) 146, 491
SmoI CTYRAG 2 cut(s) 146, 491
SsiI CCGC 2 cut(s) 1180, 2112
SspDI GGCGCC 1 cut(s) 2365
SspI AATATT 2 cut(s) 537, 1885
SspMI CTAG 6 cut(s) 180, 239, 356, 831, 866, 1749
StyD4I CCNGG 2 cut(s) 1975, 2116
StyI CCWWGG 1 cut(s) 2019
TaaI ACNGT 9 cut(s) 46, 1325, 1621, 1651, 1910, 2506, 2613, 2645, 2807
TaiI ACGT 2 cut(s) 237, 2814
TaqII GACCGA 1 cut(s) 896
TatI WGTACW 2 cut(s) 1328, 2290
TauI GCSGC 1 cut(s) 2115
TfiI GAWTC 4 cut(s) 1448, 1670, 1736, 1767
TscAI CASTG 4 cut(s) 259, 1915, 1977, 2618
TseFI GTSAC 3 cut(s) 958, 1383, 2807
TseI GCWGC 4 cut(s) 163, 1154, 1806, 2697
Tsp45I GTSAC 3 cut(s) 958, 1383, 2807
TspGWI ACGGA 3 cut(s) 1623, 1628, 2499
TspRI CASTG 4 cut(s) 259, 1915, 1977, 2618
Vha464I CTTAAG 1 cut(s) 491
VpaK11BI GGWCC 4 cut(s) 610, 879, 1294, 1636
VspI ATTAAT 1 cut(s) 1998
XapI RAATTY 7 cut(s) 110, 1684, 1798, 1918, 2194, 2432, 2516
XbaI TCTAGA 2 cut(s) 830, 865
XhoI CTCGAG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 2481
XmnI GAANNNNTTC 1 cut(s) 1827
XspI CTAG 6 cut(s) 180, 239, 356, 831, 866, 1749
ZraI GACGTC 1 cut(s) 2812
Zsp2I ATGCAT 1 cut(s) 1536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.