RchiOBHm_Chr5g0020111

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003630
Physical Location & Seq
Reverse (-)
14412115 .. 14417597
5483 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30022

Sequence Viewer

Length: 3744 bp
ATGGTTGTCATGATTAGTGAATTTGACAGCCTTTTGCTGAGTACACCAATGGAGTCAGATGAGGAGCACAAGACTGTTCAAGGAATGTCTCCCGAATATGGTGCAATTTTCATGTCAAGCAGTTTTACAAAAGATGAATGTTTTAGACGAAGACTGTTTGGCCTTCCATCTGGACAAGGCCCATTTGTAAAGCAGATCAAAAGTGGAATGATTTTATTTCTCTTTGAATTTGAGAAGAGAGAACTCCATGGTGTATTCCAGGCATCCTCTGATGGTAGAATGAATATTGTTCCTAATGCATGGAGTAGATCAGGAAAGAAGTTTCCTGCTCAGGTGAGAGTCAACCCAATTTGGCATTGTCATCCACTTCCAGAATCTGAGTTTGGTGACGCAATCAAAGAAAACTATTTTTCAAAATGGAAATTCAATTTTGGTCTTTCCAAAGCTCAGGTTTGGAGGCTTCTAATGTTGTTTAATTTGCGAAAATTGAAAAGTCCACATCCTCAGAGAGCACTAGCTAGAAATACTGCTGCACAACCGGTGGACATTTATCGAGAACTGGATGATGGCAGATTATCAAGTGATAAAGTGGGTAATCTACATAAAGTGGACAATATGCTTCGGTCGATCATCGAGAGCAGGTATCTTGAACACCTGCCAGGCAAAGTTGAAAGGGATGATGCTAAGTTTGGGAATGGAGTTAATGTGGATTTTGAACACAGGGCATTGCCATCAACTGAGCATACTAGGTCAGGCCAAAATGGAAGATGTGATGGCAGCCTGGCAATGTGTGGAAGGGAAGGAAGTGTCAGTCACTTGGATGGTCCTTTTTATTCTGGAATGCCTCTTCAAGAAACATATTCTTTGGTTCAAGATAAAGAAACTTCTAGCCATCAGATGGAGCTGCCAATCACTGGCCACTCTTATGCAGCATCTCGTGGAGATGCAATTATTACGAGCATTCTTCCCTATGATCCTGATGATCCTAGATTGAACCTTGCACATTCTCTATTAGTTGGAAATTATGATTCTGTTCAAGACTGTGATGGCCAGAATGGCATTCCCGCGGTGAGGAATGAAGTAAATCCCTCATACACAGAGACAAATTCACTAAATCAAAGCCTAGAAGACGTTTCTAAGGTTGATGTTCGCAGATCACTTTCAGCCATCAATATTACTCCTTTTCCAGGGGCAACTTCTTCAGCAGAAATGGCTATTTACTATTCAAAGTCCTCTTTAGCTCCTGAATCTTTATCAGAATTTGGAAGTAATGGGAGGGACGGGCCTTCCTCTTATCCCCTTAGCCCCAGTGATTATCCTTCCTTTCTGGTTGACCGCAGGCATCCTGTAGCAATACAAAATAAACCAGTGCATGAAATAGGACCATTTACTGCAAATGTAACTTCATTAGAGGAGATACAGTGCCAGTCACCATGCCGCACTCATTCTGCTGACCTTGACAGTGTGGACTACCACGACTGTAAATGTTCTGATCATAAAATTTCTGATAGAATGCTGTGTTCAGATCTTCTGGAAAACAGAAGTAGTGTGTTCTCTCGCCTGAGATTCAATAACCCTGGATGGTGTCTGGAAAACCTTGGACATCTTGGCCACGAGGAAGATGTTAAAGACACGTCATCAGTGGATGAAGTCATGGAAATGTTGAGCGAGAGTCACCATGCCTGGATGGAGAGTGAAAAATCTAAACTGCATAAGAGACGACAAGGTAATGTAGAGAAGTCTAGGAATTTGAAGCAGACAGCTGTGAAGTCTGAGTTGGAAATGATTCCAGAGAAGGCAAACACAGCTTCTGCTTCACCAACTGGAGATAAAGATGATCAAAGCAGTAAAAAGACACTGTTTGTGGACTTCAAGCGGCGGAGTGATTTGCGAAAAGTCAATAGCGATACTAATGGGGAAAGTGCTGGAAGCGAGGGATTGTTAGGTGGGCAGTGCAAGAGAAGGAAGTTAATCAGGCCTAACTTCAGTGAGAATGAGCCATGTGATAACAAAAGCTATAATAGAAGTCATTTTATGAATTCACCAGGATCAGCCCAAGAATGCCGTAGTTGTGAAGATGCTGGTGGCAAGAAAAGGAAGCTGGCTAAGCCAAATTTCAGAGAGAACGAGTCATCTCAGGAATCATCTGCGCAATGTTCTATTGGTGAAGATGCTGGTGGCAAGAGAAGGAAGCTGGCTAGGCCAAATTTTAGAGAAAATGAGTCGTCTTTAATATCCTCGCCACAATGTTCTGTTGGTGAAGATGCTGGTGGAAGTTGCGGGTCTTTTGTTGGAAGCCAAGGTAAGTCGTTATTACAAGGTGCAGAATCTTCACACGACAATGAGAAGATCGATACTGAATGTGTTTCTAAATATGAAACAGAAATTATAGTAGAAAGTTCTGGGGGTTCTCCCAAAATTGGAGATGATAGTGGAAAGGGGTGTTTGCATGCCTATCCTGGAAATTCTTGTCTGAACTTACAAGCATCTGTCGATGAATCTTCTTTTTGTGAAGATGCTAATTGTAAGGAGCTAGTTGGGCCTGATTTCAGAGAGAATGAGCTATTCCGAGTGTCGCCACATATATGTTCTATTGGTGAATATGCTGGTGAAAAGTATGAGGCATTGGTTGGAAGCCAAGGTAACACTGATGCTCAAATATTTCCAACATTTAAAATGGAAATTAAACAATTAAGCTCTGGGGAGTCTCTCAAAATTGGAGATGAAAGTGGAAAGAGTCCTGTGCATGATGTTGGCTGCCAAATTGTCATCGGCCCTTCCAAAGATGATATTCTTAATGCACAGGAAGACTTGGATCACAACGACCCTATGGTGTTGTCTCGAGATGGTCATCAGCCCAGTCAATCCACCGTGCTAAACTCGGCTTCGCAACCCAAGACAAATGGATTTTCCGAATTTGAAAGTGACAGAGGCAGTGAACTGGAGATGCTTCCAAGGGTTGAATTTTCTCCAAACATAGTTGGCAATGGTGGCTCAAGGAAGCAGAACCCTGACTCACCAAGAGTAGCTGAAAACATCACTAATCGCGTGGATGCGGGTATGATAAATTATCAACACGGATCCAGTACTGAGCAGGGTCAGGCATCTTGCAACTCCAACCAGCATGATTCACTTAAACAGGAAGGGTTCGGGAATGACGTGCTGGAGGCTGTGGAGTTTGGTGATAATGTTGCAAGTGATAACACTTCAAGAGAAAGAGAAATAGACATTAAACTAGAAAGCTTTGTGGATTCTGTCAAAAGTGGAGATGAAAATGGAATGGAGAGCGTGCAGGTTGGTATTCTTGGTGCACTGGAAGACCCTATACTTACCAGGTTGTCTGAAGACTTTCCCGGGACCGATCAGATAACTGTGCTAGAGTCAGCTACGCAACCTAAGACGAATTCTGGAACAAGTAATGGATTTTCTGAAATCGAAGGTGACAGAGGCAGTGAACTGCAGGTGCTTCCACTAGTTGAACGTTTTCCAAATATAAACGCCTCTGATAGTGCAGATGCAAGTATGGGAAATAACCAACAAGGAGTCAATTCTGAGCAGTTGCAGACGTCTTGCAACTCCAACAATCACAGATCACTTCAAGAGCATGGATCCAAACATAACGTGCAAGAGAGCATGGAGTTCGGCGATAATGAGGCAATTGATGACACTGTAAGAGAAAATAAGAGGCTATGGAGTATTTTAACAGCTAGATTGCAGCTTGTTAAGAGGGCACAACAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

1247

Amino Acids

137.39

Weight (kDa)

5.18

Isoelectric Point (pI)

53.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 34 - 158 1.9e-48 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 663, 3451
AatII GACGTC 1 cut(s) 3569
Acc16I TGCGCA 1 cut(s) 2151
Acc36I ACCTGC 4 cut(s) 630, 663, 3283, 3451
AccB7I CCANNNNNTGG 2 cut(s) 898, 914
AccII CGCG 2 cut(s) 1067, 3048
AciI CCGC 8 cut(s) 1065, 1067, 1336, 1438, 1876, 1879, 2280, 3056
AclI AACGTT 1 cut(s) 3481
AclWI GGATC 8 cut(s) 968, 977, 2056, 2823, 3075, 3088, 3603, 3616
AcoI YGGCCR 3 cut(s) 916, 1048, 1609
AcuI CTGAAG 3 cut(s) 1185, 1969, 3363
AcyI GRCGYC 1 cut(s) 3566
AfaI GTAC 2 cut(s) 43, 3088
AfiI CCNNNNNNNGG 6 cut(s) 98, 898, 914, 1071, 1187, 2420
AflIII ACRYGT 1 cut(s) 1632
AgeI ACCGGT 1 cut(s) 538
AhlI ACTAGT 1 cut(s) 3472
AjiI CACGTC 2 cut(s) 1635, 3160
AjnI CCWGG 9 cut(s) 258, 658, 780, 1186, 1576, 1682, 2044, 2458, 3332
AjuI GAANNNNNNNTTGG 4 cut(s) 366, 398, 1760, 1792
Alw21I GWGCWC 3 cut(s) 69, 514, 3313
Alw26I GTCTC 5 cut(s) 93, 1094, 1711, 2712, 2844
Alw44I GTGCAC 1 cut(s) 3309
AlwI GGATC 8 cut(s) 968, 977, 2056, 2823, 3075, 3088, 3603, 3616
AlwNI CAGNNNCTG 2 cut(s) 377, 1811
Ama87I CYCGRG 2 cut(s) 2841, 3353
ApaLI GTGCAC 1 cut(s) 3309
ApeKI GCWGC 6 cut(s) 530, 777, 904, 929, 2757, 3715
AsiGI ACCGGT 1 cut(s) 538
Asp700I GAANNNNTTC 3 cut(s) 1785, 3348, 3483
AspLEI GCGC 1 cut(s) 2152
AspS9I GGNCC 7 cut(s) 179, 824, 1283, 1382, 2540, 2774, 3357
AsuC2I CCSGG 2 cut(s) 3354, 3355
AvaI CYCGRG 2 cut(s) 2841, 3353
AvaII GGWCC 3 cut(s) 824, 1382, 3357
BaeGI GKGCMC 2 cut(s) 3313, 3733
BalI TGGCCA 3 cut(s) 918, 1050, 1611
BamHI GGATCC 2 cut(s) 3080, 3608
BarI GAAGNNNNNNTAC 2 cut(s) 3309, 3341
BauI CACGAG 2 cut(s) 936, 1613
BbsI GAAGAC 5 cut(s) 157, 1134, 2814, 3324, 3351
Bbv12I GWGCWC 3 cut(s) 69, 514, 3313
BbvI GCAGC 6 cut(s) 517, 789, 891, 941, 2744, 3727
BceAI ACGGC 1 cut(s) 2049
BcgI CGANNNNNNTGC 4 cut(s) 83, 117, 3622, 3656
BciT130I CCWGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3334
BclI TGATCA 2 cut(s) 1492, 1837
BcnI CCSGG 2 cut(s) 3354, 3355
BcoDI GTCTC 5 cut(s) 93, 1094, 1711, 2712, 2844
BcuI ACTAGT 1 cut(s) 3472
BfmI CTRYAG 2 cut(s) 1347, 3458
BfuAI ACCTGC 4 cut(s) 630, 663, 3283, 3451
BglI GCCNNNNNGGC 1 cut(s) 1056
BglII AGATCT 1 cut(s) 1525
BisI GCNGC 8 cut(s) 531, 778, 905, 930, 1438, 1877, 2758, 3716
BlpI GCTNAGC 1 cut(s) 2106
BlsI GCNGC 8 cut(s) 532, 779, 906, 931, 1439, 1878, 2759, 3717
BmcAI AGTACT 1 cut(s) 3088
Bme18I GGWCC 3 cut(s) 824, 1382, 3357
BmeT110I CYCGRG 2 cut(s) 2841, 3353
BmgBI CACGTC 2 cut(s) 1635, 3160
BmgT120I GGNCC 7 cut(s) 179, 824, 1283, 1382, 2540, 2774, 3357
BmiI GGNNCC 3 cut(s) 3082, 3358, 3610
BmrI ACTGGG 2 cut(s) 1302, 2853
BmuI ACTGGG 2 cut(s) 1302, 2853
BpiI GAAGAC 5 cut(s) 157, 1134, 2814, 3324, 3351
BpmI CTGGAG 3 cut(s) 1845, 2963, 3185
Bpu10I CCTNAGC 3 cut(s) 330, 447, 1301
Bpu1102I GCTNAGC 1 cut(s) 2106
BpuEI CTTGAG 1 cut(s) 2980
BpuMI CCSGG 2 cut(s) 3354, 3355
Bsa29I ATCGAT 1 cut(s) 2352
BsaBI GATNNNNATC 1 cut(s) 2850
BsaHI GRCGYC 1 cut(s) 3566
BsaJI CCNNGG 9 cut(s) 247, 1065, 1187, 1576, 1597, 2299, 2638, 2954, 3353
BsaWI WCCGGW 1 cut(s) 538
BsaXI ACNNNNNCTCC 4 cut(s) 690, 720, 1406, 1436
Bsc4I CCNNNNNNNGG 6 cut(s) 98, 898, 914, 1071, 1187, 2420
Bse118I RCCGGY 1 cut(s) 538
Bse3DI GCAATG 4 cut(s) 725, 792, 2159, 2992
Bse8I GATNNNNATC 1 cut(s) 2850
BseBI CCWGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3334
BseCI ATCGAT 1 cut(s) 2352
BseDI CCNNGG 9 cut(s) 247, 1065, 1187, 1576, 1597, 2299, 2638, 2954, 3353
BseJI GATNNNNATC 1 cut(s) 2850
BseLI CCNNNNNNNGG 6 cut(s) 98, 898, 914, 1071, 1187, 2420
BseMI GCAATG 4 cut(s) 725, 792, 2159, 2992
BseRI GAGGAG 2 cut(s) 77, 1427
BseSI GKGCMC 2 cut(s) 3313, 3733
BseXI GCAGC 6 cut(s) 517, 789, 891, 941, 2744, 3727
BsgI GTGCAG 4 cut(s) 516, 2343, 3311, 3531
Bsh1236I CGCG 2 cut(s) 1067, 3048
Bsh1285I CGRYCG 1 cut(s) 626
BshTI ACCGGT 1 cut(s) 538
BshVI ATCGAT 1 cut(s) 2352
BsiEI CGRYCG 1 cut(s) 626
BsiHKAI GWGCWC 3 cut(s) 69, 514, 3313
BsiHKCI CYCGRG 2 cut(s) 2841, 3353
BsiSI CCGG 2 cut(s) 539, 3354
BslFI GGGAC 2 cut(s) 1292, 3370
BslI CCNNNNNNNGG 6 cut(s) 98, 898, 914, 1071, 1187, 2420
BsmAI GTCTC 5 cut(s) 93, 1094, 1711, 2712, 2844
BsmBI CGTCTC 1 cut(s) 1711
BsmFI GGGAC 2 cut(s) 1292, 3370
BsmI GAATGC 5 cut(s) 846, 960, 1059, 1518, 2066
BsoBI CYCGRG 2 cut(s) 2841, 3353
Bsp1286I GDGCHC 4 cut(s) 69, 514, 3313, 3733
Bsp1720I GCTNAGC 1 cut(s) 2106
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 8 cut(s) 1065, 1067, 1336, 1438, 1876, 1879, 2280, 3056
BspDI ATCGAT 1 cut(s) 2352
BspFNI CGCG 2 cut(s) 1067, 3048
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 3 cut(s) 3082, 3358, 3610
BspMAI CTGCAG 1 cut(s) 3462
BspMI ACCTGC 4 cut(s) 630, 663, 3283, 3451
BspPI GGATC 8 cut(s) 968, 977, 2056, 2823, 3075, 3088, 3603, 3616
BsrDI GCAATG 4 cut(s) 725, 792, 2159, 2992
BsrFI RCCGGY 1 cut(s) 538
BssAI RCCGGY 1 cut(s) 538
BssECI CCNNGG 9 cut(s) 247, 1065, 1187, 1576, 1597, 2299, 2638, 2954, 3353
BssNI GRCGYC 1 cut(s) 3566
BssSI CACGAG 2 cut(s) 936, 1613
BssT1I CCWWGG 5 cut(s) 247, 1597, 2299, 2638, 2954
Bst2BI CACGAG 2 cut(s) 936, 1613
Bst2UI CCWGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3334
Bst6I CTCTTC 2 cut(s) 230, 852
BstACI GRCGYC 1 cut(s) 3566
BstC8I GCNNGC 5 cut(s) 1340, 2103, 2196, 2451, 3290
BstDSI CCRYGG 2 cut(s) 247, 1065
BstENI CCTNNNNNAGG 1 cut(s) 1185
BstFNI CGCG 2 cut(s) 1067, 3048
BstHHI GCGC 1 cut(s) 2152
BstMAI GTCTC 5 cut(s) 93, 1094, 1711, 2712, 2844
BstMCI CGRYCG 1 cut(s) 626
BstMWI GCNNNNNNNGC 7 cut(s) 1056, 1211, 1805, 2107, 2200, 2539, 2991
BstNI CCWGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3334
BstNSI RCATGY 1 cut(s) 2453
BstSFI CTRYAG 2 cut(s) 1347, 3458
BstSLI GKGCMC 2 cut(s) 3313, 3733
BstUI CGCG 2 cut(s) 1067, 3048
BstV1I GCAGC 6 cut(s) 517, 789, 891, 941, 2744, 3727
BstV2I GAAGAC 5 cut(s) 157, 1134, 2814, 3324, 3351
BstX2I RGATCY 3 cut(s) 1525, 3080, 3608
BstYI RGATCY 3 cut(s) 1525, 3080, 3608
Bsu15I ATCGAT 1 cut(s) 2352
BsuTUI ATCGAT 1 cut(s) 2352
BtgI CCRYGG 2 cut(s) 247, 1065
BtrI CACGTC 2 cut(s) 1635, 3160
BtsI GCAGTG 3 cut(s) 1958, 2941, 3457
BveI ACCTGC 4 cut(s) 630, 663, 3283, 3451
Cac8I GCNNGC 5 cut(s) 1340, 2103, 2196, 2451, 3290
CaiI CAGNNNCTG 2 cut(s) 377, 1811
CciI TCATGA 1 cut(s) 9
CfoI GCGC 1 cut(s) 2152
Cfr10I RCCGGY 1 cut(s) 538
Cfr13I GGNCC 7 cut(s) 179, 824, 1283, 1382, 2540, 2774, 3357
Cfr42I CCGCGG 1 cut(s) 1068
Cfr9I CCCGGG 1 cut(s) 3353
ClaI ATCGAT 1 cut(s) 2352
CseI GACGC 1 cut(s) 398
CsiI ACCWGGT 1 cut(s) 3332
Csp6I GTAC 2 cut(s) 42, 3087
CspAI ACCGGT 1 cut(s) 538
CviQI GTAC 2 cut(s) 42, 3087
DraI TTTAAA 1 cut(s) 2674
EaeI YGGCCR 3 cut(s) 916, 1048, 1609
Eam1104I CTCTTC 2 cut(s) 230, 852
EarI CTCTTC 2 cut(s) 230, 852
EciI GGCGGA 1 cut(s) 1894
Eco130I CCWWGG 5 cut(s) 247, 1597, 2299, 2638, 2954
Eco147I AGGCCT 1 cut(s) 1978
Eco47I GGWCC 3 cut(s) 824, 1382, 3357
Eco57I CTGAAG 3 cut(s) 1185, 1969, 3363
Eco88I CYCGRG 2 cut(s) 2841, 3353
EcoNI CCTNNNNNAGG 1 cut(s) 1185
EcoRI GAATTC 2 cut(s) 2038, 3403
EcoRII CCWGG 9 cut(s) 258, 658, 780, 1186, 1576, 1682, 2044, 2458, 3332
EcoT14I CCWWGG 5 cut(s) 247, 1597, 2299, 2638, 2954
EcoT22I ATGCAT 1 cut(s) 301
ErhI CCWWGG 5 cut(s) 247, 1597, 2299, 2638, 2954
Esp3I CGTCTC 1 cut(s) 1711
FaqI GGGAC 2 cut(s) 1292, 3370
FauI CCCGC 3 cut(s) 1072, 2273, 3049
FbaI TGATCA 2 cut(s) 1492, 1837
Fnu4HI GCNGC 8 cut(s) 531, 778, 905, 930, 1438, 1877, 2758, 3716
Fsp4HI GCNGC 8 cut(s) 531, 778, 905, 930, 1438, 1877, 2758, 3716
FspI TGCGCA 1 cut(s) 2151
GlaI GCGC 1 cut(s) 2151
GluI GCNGC 8 cut(s) 531, 778, 905, 930, 1438, 1877, 2758, 3716
GsuI CTGGAG 3 cut(s) 1845, 2963, 3185
HapII CCGG 2 cut(s) 539, 3354
HgaI GACGC 1 cut(s) 398
HhaI GCGC 1 cut(s) 2152
Hin1I GRCGYC 1 cut(s) 3566
Hin6I GCGC 1 cut(s) 2150
HinP1I GCGC 1 cut(s) 2150
HincII GTYRAC 2 cut(s) 343, 1333
HindII GTYRAC 2 cut(s) 343, 1333
HindIII AAGCTT 1 cut(s) 3241
HpaII CCGG 2 cut(s) 539, 3354
HpyCH4IV ACGT 6 cut(s) 1131, 1634, 3159, 3481, 3566, 3621
HpyF10VI GCNNNNNNNGC 7 cut(s) 1056, 1211, 1805, 2107, 2200, 2539, 2991
HpySE526I ACGT 6 cut(s) 1131, 1634, 3159, 3481, 3566, 3621
Hsp92I GRCGYC 1 cut(s) 3566
HspAI GCGC 1 cut(s) 2150
Ksp22I TGATCA 2 cut(s) 1492, 1837
KspI CCGCGG 1 cut(s) 1068
LmnI GCTCC 4 cut(s) 64, 901, 1246, 2530
Lsp1109I GCAGC 6 cut(s) 517, 789, 891, 941, 2744, 3727
MabI ACCWGGT 1 cut(s) 3332
MaeII ACGT 6 cut(s) 1131, 1634, 3159, 3481, 3566, 3621
MaeIII GTNAC 8 cut(s) 386, 812, 1399, 1428, 1673, 2642, 2924, 3440
MfeI CAATTG 1 cut(s) 3657
MflI RGATCY 3 cut(s) 1525, 3080, 3608
MhlI GDGCHC 4 cut(s) 69, 514, 3313, 3733
MlsI TGGCCA 3 cut(s) 918, 1050, 1611
MluNI TGGCCA 3 cut(s) 918, 1050, 1611
MmeI TCCRAC 7 cut(s) 997, 1758, 2272, 2611, 2690, 3141, 3603
Mox20I TGGCCA 3 cut(s) 918, 1050, 1611
Mph1103I ATGCAT 1 cut(s) 301
MroXI GAANNNNTTC 3 cut(s) 1785, 3348, 3483
MscI TGGCCA 3 cut(s) 918, 1050, 1611
MslI CAYNNNNRTG 5 cut(s) 819, 924, 1658, 2340, 2584
Msp20I TGGCCA 3 cut(s) 918, 1050, 1611
MspA1I CMGCKG 2 cut(s) 1067, 1763
MspI CCGG 2 cut(s) 539, 3354
MunI CAATTG 1 cut(s) 3657
Mva1269I GAATGC 5 cut(s) 846, 960, 1059, 1518, 2066
MvaI CCWGG 9 cut(s) 260, 660, 782, 1188, 1578, 1684, 2046, 2460, 3334
MvnI CGCG 2 cut(s) 1067, 3048
MwoI GCNNNNNNNGC 7 cut(s) 1056, 1211, 1805, 2107, 2200, 2539, 2991
NciI CCSGG 2 cut(s) 3354, 3355
NcoI CCATGG 1 cut(s) 247
NlaIV GGNNCC 3 cut(s) 3082, 3358, 3610
NmeAIII GCCGAG 1 cut(s) 2861
NmuCI GTSAC 6 cut(s) 386, 812, 1428, 1673, 2924, 3440
NsbI TGCGCA 1 cut(s) 2151
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 1 cut(s) 2453
PaeI GCATGC 1 cut(s) 2453
PaeR7I CTCGAG 1 cut(s) 2841
PagI TCATGA 1 cut(s) 9
PaqCI CACCTGC 2 cut(s) 663, 3451
PceI AGGCCT 1 cut(s) 1978
PcsI WCGNNNNNNNCGW 1 cut(s) 3156
PctI GAATGC 5 cut(s) 846, 960, 1059, 1518, 2066
PdmI GAANNNNTTC 3 cut(s) 1785, 3348, 3483
PflMI CCANNNNNTGG 2 cut(s) 898, 914
PfoI TCCNGGA 1 cut(s) 2458
PinAI ACCGGT 1 cut(s) 538
PkrI GCNGC 8 cut(s) 532, 779, 906, 931, 1439, 1878, 2759, 3717
Psp1406I AACGTT 1 cut(s) 3481
Psp6I CCWGG 9 cut(s) 258, 658, 780, 1186, 1576, 1682, 2044, 2458, 3332
PspGI CCWGG 9 cut(s) 258, 658, 780, 1186, 1576, 1682, 2044, 2458, 3332
PspN4I GGNNCC 3 cut(s) 3082, 3358, 3610
PspPI GGNCC 7 cut(s) 179, 824, 1283, 1382, 2540, 2774, 3357
PstI CTGCAG 1 cut(s) 3462
PstNI CAGNNNCTG 2 cut(s) 377, 1811
PsuI RGATCY 3 cut(s) 1525, 3080, 3608
PvuII CAGCTG 1 cut(s) 1763
RsaI GTAC 2 cut(s) 43, 3088
RsaNI GTAC 2 cut(s) 42, 3087
RseI CAYNNNNRTG 5 cut(s) 819, 924, 1658, 2340, 2584
SacII CCGCGG 1 cut(s) 1068
SatI GCNGC 8 cut(s) 531, 778, 905, 930, 1438, 1877, 2758, 3716
Sau96I GGNCC 7 cut(s) 179, 824, 1283, 1382, 2540, 2774, 3357
ScaI AGTACT 1 cut(s) 3088
SduI GDGCHC 4 cut(s) 69, 514, 3313, 3733
SexAI ACCWGGT 1 cut(s) 3332
SfcI CTRYAG 2 cut(s) 1347, 3458
Sfr274I CTCGAG 1 cut(s) 2841
Sfr303I CCGCGG 1 cut(s) 1068
SgrBI CCGCGG 1 cut(s) 1068
SinI GGWCC 3 cut(s) 824, 1382, 3357
SlaI CTCGAG 1 cut(s) 2841
SmaI CCCGGG 1 cut(s) 3355
SmiMI CAYNNNNRTG 5 cut(s) 819, 924, 1658, 2340, 2584
SmlI CTYRAG 2 cut(s) 2841, 2995
SmoI CTYRAG 2 cut(s) 2841, 2995
SpeI ACTAGT 1 cut(s) 3472
SphI GCATGC 1 cut(s) 2453
SseBI AGGCCT 1 cut(s) 1978
SsiI CCGC 8 cut(s) 1065, 1067, 1336, 1438, 1876, 1879, 2280, 3056
SspI AATATT 3 cut(s) 286, 1174, 2661
StuI AGGCCT 1 cut(s) 1978
StyI CCWWGG 5 cut(s) 247, 1597, 2299, 2638, 2954
TaiI ACGT 6 cut(s) 1134, 1637, 3162, 3484, 3569, 3624
TaqI TCGA 7 cut(s) 553, 626, 633, 2352, 2493, 2842, 3435
TaqII GACCGA 2 cut(s) 612, 3374
TatI WGTACW 2 cut(s) 41, 3086
TauI GCSGC 2 cut(s) 1440, 1879
TseFI GTSAC 6 cut(s) 386, 812, 1428, 1673, 2924, 3440
TseI GCWGC 6 cut(s) 530, 777, 904, 929, 2757, 3715
Tsp45I GTSAC 6 cut(s) 386, 812, 1428, 1673, 2924, 3440
TspGWI ACGGA 1 cut(s) 3093
TspMI CCCGGG 1 cut(s) 3353
Van91I CCANNNNNTGG 2 cut(s) 898, 914
VneI GTGCAC 1 cut(s) 3309
VpaK11BI GGWCC 3 cut(s) 824, 1382, 3357
XagI CCTNNNNNAGG 1 cut(s) 1185
XceI RCATGY 1 cut(s) 2453
XhoI CTCGAG 1 cut(s) 2841
XmaI CCCGGG 1 cut(s) 3353
XmnI GAANNNNTTC 3 cut(s) 1785, 3348, 3483
ZraI GACGTC 1 cut(s) 3567
ZrmI AGTACT 1 cut(s) 3088
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.