pycom05g22930

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003630
Physical Location & Seq
Forward (+)
25052544 .. 25056088
3545 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22930.1

Sequence Viewer

Length: 2283 bp
ATGATTTCTGCAAGTGATAATTCTGAATTTGTTCACCTTCGATTATTTGTGTTAAATAGGAAGAAAATAACAATAATACTATTGATTTTATCAAATTATTTTGTTATATTGATTTCAAATTTCAGTGACCAAAATAAAAACTTACCCCAATCTCATCATTATTTTAGGTGTTTCATTAAGTTATTCCAAGAAAATATTCTCTTAAAATTAACTAATTTGGAAAATAATTATTGTATACAAGTACACAGTACCAAAGTTGAAAGATTTTTTGAACCCCAAAAAGAAAGTAAAAGGAAACGAAAAAAAAAAGAAAGAAAAAAAAAACGACACCGGATGATCCATGTCGATAGTGAGTTAAAAACCGCTATGGGGTCCCACTCACACACGACGTCGTATAAAATCGTAGAGCCAAAAGTTCTGATAGCTCACGCCACTCTCGAGAAGCCACACCGGCACACGCCGTCGTCTTCTTCTCTCTTCCACCTCTTCCTCTTCTCTCTTTCTTCTGCACCAATGGAGTCAGACGAGGAGCAGGATTGTTTTCGCGGGTTATATCCGGAGTATGGTGCAATTTTCATGTCAAGCACTGCAACGATAGATGAGTGTTTTAAACGGAGACTGTTTGGCCTTCCATCTTCACAAGGCCAATTTGTAAAGCAGATTAAAACTGGAATGATTTTGTTTCTGTTTGAATTCGAGAGGAGAGAACTTCATGGTGTTTTTCAAGCATGCTCTGATGGTGAAATGAATATTTCGCCTTCTGCATACAATTCATCAGGAAGGCAGTTTCCTGCTCAGGTAAAAGTCAAGCTTATTTGGCGTTGTCATTCGCTCACAGAACCTGAATTCAGTGGCGCAATCAAGGATAACTATTTTTCAAATTGGAAGTTCAATTTCGGTCTGTCCAAAGCTCAGGTTCGAAGGCTTCTAATGTTGTTCAGTTCACGAAAGTTGAAATATCAGCGGCGGCAGAGAGAATTAGCCAGTAGAAGAGAAGCAATATCAGTGGACACTGCTGGTAGAGTCAAGGAAGTTGATGATGGCAAAACTCTATTGAGTGATAAGGCAACCAACGAGCTTGTTGCCAACAATTATAAGGGGCCAAAAATGACTGGGTCACCGGGGAATGTCTCTGGGAATATTGGAAAAGGAGATGGTGGCAGGTTGGCAAGTGATGAAGTGGGTAATCTTCATGAAGTAGACAAGAAGGTTGGGGAGATCATGCAGAGTGAGTATCTTGTGCCCGTTCCAGGTGAAGTTGGACAATTGGTTGCTACTTTTGCAATGTCTGAGAGGGCAGGGAATGAATTTAATGTGGATGTTGAATCCGTGTCATCTGAGCATCCTATGTCGGGTCAATCTGGAAATGGTGATGACGACCTGGTAATTCATGATGATTCCATCTGTTTAATGGGTGATCAATTACAAATCGGCAATACTGAAAGTAATGTTTTTGGGCCAGCTGTACCAACTGAGAACCCTGCTTTCTTTCAGTCTAGTCTTGATAGACCTGTCTGTGCTGGCATGCCTGTTGAAGACACAGGTTCTTTGATTCAAGATCAAACTAGATCAACTTCAAATGTTGGCCATCTAATGGAGCTGCCAATTGCTGGCCATTCTTGTTCACCATCTCTGTCTTATAGAGATGCGATTGTAACCAGCACCTATGATCCTGATGCTCTTAGTCTGAACAATCCACGCTCACAATCAATTGGGGTCAATCAGAGTTCAAATTCCGTTCAAGATTGTTATAAACAACATGGCATTCCTTCCATTGCAAATCAATCATACACGGAGCCAAAAGGAATCAATCAAAGCCTAGAGTCCAATCCAAAGTTTGATGTTCATGTTCCATTCGCATCCTCTGACCATTATGAGCACACATGTCATGGGAACATCATGCCTTTTCCAGGGGTGGCATATTCTGAAGAAATGACCATAGATTATTCTGGAAAACATTGTTCCTGGGACCCAGTTTTGAAGAGTTTTTTATCAACTGACAGTTTTTCACAAATTGGAAGTAGCGGGAGGGAAACCAAGAGGCCAACATCTTATTCTCTCAGTCCTAGTAACTGTCTGTCCTTTGTGATTGATCGTGCTCATCCTGTAGCATTACAAAAGAAACTTGAGCAAGAAATGGCACAGAAATTGAACAATGAACCATGTACTGCTAATGTAACTTCATCTGAGGAGCTTCAGTGCCAGTTGCAATGTCGCATTCATCCTGATGGTCATAGCGCTAGCAGCGTGGACCATCGTAAACAGAAATCTTTTGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

761

Amino Acids

85.14

Weight (kDa)

7.16

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 189 - 313 5.2e-47 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1095, 1752
AatII GACGTC 1 cut(s) 392
Acc36I ACCTGC 1 cut(s) 1152
AccB7I CCANNNNNTGG 2 cut(s) 1594, 1610
AccI GTMKAC 2 cut(s) 235, 1200
AccII CGCG 1 cut(s) 546
AccIII TCCGGA 1 cut(s) 556
AciI CCGC 5 cut(s) 363, 546, 964, 967, 2025
AclWI GGATC 2 cut(s) 331, 1664
AcoI YGGCCR 2 cut(s) 1585, 1612
AcsI RAATTY 6 cut(s) 26, 118, 692, 845, 1307, 1732
AcuI CTGAAG 2 cut(s) 1947, 2180
AcyI GRCGYC 1 cut(s) 389
AfaI GTAC 4 cut(s) 243, 250, 1467, 2167
AfeI AGCGCT 1 cut(s) 2239
AfiI CCNNNNNNNGG 7 cut(s) 369, 563, 1250, 1352, 1594, 1610, 1910
AflIII ACRYGT 1 cut(s) 1883
AjnI CCWGG 4 cut(s) 1249, 1380, 1909, 1964
AjuI GAANNNNNNNTTGG 4 cut(s) 180, 212, 2038, 2070
AluBI AGCT 7 cut(s) 425, 811, 911, 1078, 1463, 1600, 2194
AluI AGCT 7 cut(s) 425, 811, 911, 1078, 1463, 1600, 2194
Alw21I GWGCWC 2 cut(s) 1881, 2101
Alw26I GTCTC 2 cut(s) 610, 1135
AlwI GGATC 2 cut(s) 331, 1664
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 556
Aor51HI AGCGCT 1 cut(s) 2239
AoxI GGCC 7 cut(s) 625, 643, 1100, 1457, 1585, 1612, 2042
ApeKI GCWGC 2 cut(s) 1600, 2244
ApoI RAATTY 6 cut(s) 26, 118, 692, 845, 1307, 1732
Asp700I GAANNNNTTC 2 cut(s) 30, 195
AspLEI GCGC 2 cut(s) 857, 2240
AspS9I GGNCC 5 cut(s) 372, 1100, 1457, 1969, 2251
AsuC2I CCSGG 1 cut(s) 1122
AsuHPI GGTGA 7 cut(s) 26, 752, 1110, 1265, 1382, 1427, 1617
AsuII TTCGAA 1 cut(s) 919
AsuNHI GCTAGC 1 cut(s) 2240
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 3 cut(s) 372, 1969, 2251
BaeGI GKGCMC 1 cut(s) 1245
BalI TGGCCA 2 cut(s) 1587, 1614
BbsI GAAGAC 2 cut(s) 459, 1542
Bbv12I GWGCWC 2 cut(s) 1881, 2101
BbvI GCAGC 2 cut(s) 1587, 2256
BccI CCATC 9 cut(s) 640, 731, 1034, 1148, 1409, 1596, 1636, 2222, 2262
BceAI ACGGC 1 cut(s) 445
BcgI CGANNNNNNTGC 2 cut(s) 1064, 1098
BciT130I CCWGG 4 cut(s) 1251, 1382, 1911, 1966
BclI TGATCA 1 cut(s) 1417
BcnI CCSGG 1 cut(s) 1122
BcoDI GTCTC 2 cut(s) 610, 1135
BfaI CTAG 5 cut(s) 1497, 1566, 1820, 2067, 2241
BfmI CTRYAG 1 cut(s) 2106
BfoI RGCGCY 1 cut(s) 2241
BfuAI ACCTGC 1 cut(s) 1152
BglI GCCNNNNNGGC 1 cut(s) 451
BisI GCNGC 4 cut(s) 965, 968, 1601, 2245
BlsI GCNGC 4 cut(s) 966, 969, 1602, 2246
Bme1390I CCNGG 5 cut(s) 1122, 1251, 1382, 1911, 1966
Bme18I GGWCC 3 cut(s) 372, 1969, 2251
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 5 cut(s) 372, 1100, 1457, 1969, 2251
BmiI GGNNCC 6 cut(s) 373, 374, 1101, 1797, 1970, 1971
BmrFI CCNGG 5 cut(s) 1122, 1251, 1382, 1911, 1966
BmrI ACTGGG 2 cut(s) 1122, 1967
BmsI GCATC 4 cut(s) 1351, 1636, 1666, 1868
BmtI GCTAGC 1 cut(s) 2244
BmuI ACTGGG 2 cut(s) 1122, 1967
BpiI GAAGAC 2 cut(s) 459, 1542
Bpu10I CCTNAGC 2 cut(s) 795, 912
Bpu14I TTCGAA 1 cut(s) 919
BpuEI CTTGAG 1 cut(s) 2147
BpuMI CCSGG 1 cut(s) 1122
Bsa29I ATCGAT 1 cut(s) 2278
BsaHI GRCGYC 1 cut(s) 389
BsaJI CCNNGG 3 cut(s) 1121, 1910, 1965
BsaWI WCCGGW 2 cut(s) 330, 556
BsaXI ACNNNNNCTCC 2 cut(s) 2183, 2213
Bsc4I CCNNNNNNNGG 7 cut(s) 369, 563, 1250, 1352, 1594, 1610, 1910
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 5 cut(s) 673, 984, 1117, 1973, 2203
Bse3DI GCAATG 3 cut(s) 1290, 1773, 2216
BseAI TCCGGA 1 cut(s) 556
BseBI CCWGG 4 cut(s) 1251, 1382, 1911, 1966
BseCI ATCGAT 1 cut(s) 2278
BseDI CCNNGG 3 cut(s) 1121, 1910, 1965
BseGI GGATG 6 cut(s) 339, 1324, 1342, 1859, 2101, 2221
BseLI CCNNNNNNNGG 7 cut(s) 369, 563, 1250, 1352, 1594, 1610, 1910
BseMI GCAATG 3 cut(s) 1290, 1773, 2216
BseMII CTCAG 7 cut(s) 809, 926, 1281, 1329, 1464, 2074, 2178
BseNI ACTGG 5 cut(s) 673, 984, 1117, 1973, 2203
BseRI GAGGAG 3 cut(s) 542, 715, 2204
BseSI GKGCMC 1 cut(s) 1245
BseXI GCAGC 2 cut(s) 1587, 2256
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 546
BshFI GGCC 7 cut(s) 627, 645, 1102, 1459, 1587, 1614, 2044
BshVI ATCGAT 1 cut(s) 2278
BsiHKAI GWGCWC 2 cut(s) 1881, 2101
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 4 cut(s) 331, 451, 557, 1121
BslFI GGGAC 2 cut(s) 358, 1982
BslI CCNNNNNNNGG 7 cut(s) 369, 563, 1250, 1352, 1594, 1610, 1910
BsmAI GTCTC 2 cut(s) 610, 1135
BsmFI GGGAC 2 cut(s) 358, 1982
BsmI GAATGC 2 cut(s) 1764, 2217
BsnI GGCC 7 cut(s) 627, 645, 1102, 1459, 1587, 1614, 2044
BsoBI CYCGRG 1 cut(s) 437
Bsp119I TTCGAA 1 cut(s) 919
Bsp1286I GDGCHC 3 cut(s) 1245, 1881, 2101
Bsp13I TCCGGA 1 cut(s) 556
Bsp143I GATC 8 cut(s) 336, 1218, 1417, 1558, 1568, 1669, 2092, 2275
BspACI CCGC 5 cut(s) 363, 546, 964, 967, 2025
BspANI GGCC 7 cut(s) 627, 645, 1102, 1459, 1587, 1614, 2044
BspCNI CTCAG 7 cut(s) 808, 925, 1282, 1330, 1465, 2073, 2179
BspDI ATCGAT 1 cut(s) 2278
BspEI TCCGGA 1 cut(s) 556
BspFNI CGCG 1 cut(s) 546
BspHI TCATGA 2 cut(s) 1192, 1390
BspLI GGNNCC 6 cut(s) 373, 374, 1101, 1797, 1970, 1971
BspMI ACCTGC 1 cut(s) 1152
BspOI GCTAGC 1 cut(s) 2244
BspPI GGATC 2 cut(s) 331, 1664
BspT104I TTCGAA 1 cut(s) 919
BsrDI GCAATG 3 cut(s) 1290, 1773, 2216
BsrFI RCCGGY 1 cut(s) 450
BsrI ACTGG 5 cut(s) 673, 984, 1117, 1973, 2203
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 3 cut(s) 1121, 1910, 1965
BssMI GATC 8 cut(s) 336, 1218, 1417, 1558, 1568, 1669, 2092, 2275
BssNAI GTATAC 1 cut(s) 236
BssNI GRCGYC 1 cut(s) 389
Bst1107I GTATAC 1 cut(s) 236
Bst2UI CCWGG 4 cut(s) 1251, 1382, 1911, 1966
Bst4CI ACNGT 4 cut(s) 248, 621, 2003, 2075
Bst6I CTCTTC 5 cut(s) 482, 491, 497, 985, 1976
BstACI GRCGYC 1 cut(s) 389
BstBI TTCGAA 1 cut(s) 919
BstC8I GCNNGC 6 cut(s) 730, 1461, 1522, 1526, 1612, 2242
BstDEI CTNAG 8 cut(s) 795, 912, 1290, 1338, 1473, 1682, 2060, 2187
BstEII GGTNACC 1 cut(s) 1116
BstENI CCTNNNNNAGG 1 cut(s) 1908
BstF5I GGATG 6 cut(s) 339, 1324, 1342, 1859, 2101, 2221
BstFNI CGCG 1 cut(s) 546
BstH2I RGCGCY 1 cut(s) 2241
BstHHI GCGC 2 cut(s) 857, 2240
BstKTI GATC 8 cut(s) 339, 1221, 1420, 1561, 1571, 1672, 2095, 2278
BstMAI GTCTC 2 cut(s) 610, 1135
BstMBI GATC 8 cut(s) 336, 1218, 1417, 1558, 1568, 1669, 2092, 2275
BstMWI GCNNNNNNNGC 4 cut(s) 451, 817, 1280, 2244
BstNI CCWGG 4 cut(s) 1251, 1382, 1911, 1966
BstNSI RCATGY 3 cut(s) 732, 1528, 1887
BstPI GGTNACC 1 cut(s) 1116
BstSCI CCNGG 5 cut(s) 1120, 1249, 1380, 1909, 1964
BstSFI CTRYAG 1 cut(s) 2106
BstSLI GKGCMC 1 cut(s) 1245
BstUI CGCG 1 cut(s) 546
BstV1I GCAGC 2 cut(s) 1587, 2256
BstV2I GAAGAC 2 cut(s) 459, 1542
BstZ17I GTATAC 1 cut(s) 236
Bsu15I ATCGAT 1 cut(s) 2278
BsuRI GGCC 7 cut(s) 627, 645, 1102, 1459, 1587, 1614, 2044
BsuTUI ATCGAT 1 cut(s) 2278
BtsCI GGATG 6 cut(s) 339, 1324, 1342, 1859, 2101, 2221
BtsI GCAGTG 2 cut(s) 585, 1011
BtsIMutI CAGTG 6 cut(s) 130, 585, 856, 1011, 1011, 2204
BveI ACCTGC 1 cut(s) 1152
Cac8I GCNNGC 6 cut(s) 730, 1461, 1522, 1526, 1612, 2242
CaiI CAGNNNCTG 1 cut(s) 842
CciI TCATGA 2 cut(s) 1192, 1390
CfoI GCGC 2 cut(s) 857, 2240
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 5 cut(s) 372, 1100, 1457, 1969, 2251
ClaI ATCGAT 1 cut(s) 2278
CsiI ACCWGGT 1 cut(s) 1380
Csp6I GTAC 4 cut(s) 242, 249, 1466, 2166
CspCI CAANNNNNGTGG 2 cut(s) 987, 1022
CviQI GTAC 4 cut(s) 242, 249, 1466, 2166
DdeI CTNAG 8 cut(s) 795, 912, 1290, 1338, 1473, 1682, 2060, 2187
DpnI GATC 8 cut(s) 338, 1220, 1419, 1560, 1570, 1671, 2094, 2277
DpnII GATC 8 cut(s) 336, 1218, 1417, 1558, 1568, 1669, 2092, 2275
DraI TTTAAA 1 cut(s) 610
EaeI YGGCCR 2 cut(s) 1585, 1612
Eam1104I CTCTTC 5 cut(s) 482, 491, 497, 985, 1976
EarI CTCTTC 5 cut(s) 482, 491, 497, 985, 1976
Eco47I GGWCC 3 cut(s) 372, 1969, 2251
Eco47III AGCGCT 1 cut(s) 2239
Eco57I CTGAAG 2 cut(s) 1947, 2180
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 1116
EcoNI CCTNNNNNAGG 1 cut(s) 1908
EcoO109I RGGNCCY 2 cut(s) 372, 1969
EcoO65I GGTNACC 1 cut(s) 1116
EcoRI GAATTC 2 cut(s) 692, 845
EcoRII CCWGG 4 cut(s) 1249, 1380, 1909, 1964
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FaqI GGGAC 2 cut(s) 358, 1982
FauI CCCGC 2 cut(s) 539, 2018
FbaI TGATCA 1 cut(s) 1417
FblI GTMKAC 2 cut(s) 235, 1200
Fnu4HI GCNGC 4 cut(s) 965, 968, 1601, 2245
FokI GGATG 6 cut(s) 346, 1329, 1331, 1846, 2088, 2208
Fsp4HI GCNGC 4 cut(s) 965, 968, 1601, 2245
FspBI CTAG 5 cut(s) 1497, 1566, 1820, 2067, 2241
GlaI GCGC 2 cut(s) 856, 2239
GluI GCNGC 4 cut(s) 965, 968, 1601, 2245
HaeII RGCGCY 1 cut(s) 2241
HaeIII GGCC 7 cut(s) 627, 645, 1102, 1459, 1587, 1614, 2044
HapII CCGG 4 cut(s) 331, 451, 557, 1121
HhaI GCGC 2 cut(s) 857, 2240
Hin1I GRCGYC 1 cut(s) 389
Hin6I GCGC 2 cut(s) 855, 2238
HinP1I GCGC 2 cut(s) 855, 2238
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 7 cut(s) 518, 1023, 1325, 1397, 1552, 1806, 1823
HpaII CCGG 4 cut(s) 331, 451, 557, 1121
HphI GGTGA 7 cut(s) 26, 752, 1110, 1265, 1382, 1427, 1617
Hpy166II GTNNAC 9 cut(s) 34, 236, 244, 944, 1009, 1201, 1625, 2251, 2261
Hpy8I GTNNAC 9 cut(s) 34, 236, 244, 944, 1009, 1201, 1625, 2251, 2261
Hpy99I CGWCG 3 cut(s) 391, 394, 466
HpyAV CCTTC 7 cut(s) 47, 638, 768, 774, 915, 1201, 1779
HpyCH4III ACNGT 4 cut(s) 248, 621, 2003, 2075
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 9 cut(s) 11, 509, 569, 590, 764, 1225, 1283, 1778, 2209
HpyF10VI GCNNNNNNNGC 4 cut(s) 451, 817, 1280, 2244
HpyF3I CTNAG 8 cut(s) 795, 912, 1290, 1338, 1473, 1682, 2060, 2187
HpySE526I ACGT 1 cut(s) 389
Hsp92I GRCGYC 1 cut(s) 389
HspAI GCGC 2 cut(s) 855, 2238
KflI GGGWCCC 2 cut(s) 372, 1969
Kpn2I TCCGGA 1 cut(s) 556
Ksp22I TGATCA 1 cut(s) 1417
Kzo9I GATC 8 cut(s) 336, 1218, 1417, 1558, 1568, 1669, 2092, 2275
LmnI GCTCC 4 cut(s) 529, 1597, 1795, 2191
Lsp1109I GCAGC 2 cut(s) 1587, 2256
LweI GCATC 4 cut(s) 1351, 1636, 1666, 1868
MabI ACCWGGT 1 cut(s) 1380
MaeI CTAG 5 cut(s) 1497, 1566, 1820, 2067, 2241
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 5 cut(s) 125, 1116, 1654, 2069, 2176
MalI GATC 8 cut(s) 338, 1220, 1419, 1560, 1570, 1671, 2094, 2277
MboI GATC 8 cut(s) 336, 1218, 1417, 1558, 1568, 1669, 2092, 2275
MfeI CAATTG 3 cut(s) 1265, 1605, 1710
MhlI GDGCHC 3 cut(s) 1245, 1881, 2101
MlsI TGGCCA 2 cut(s) 1587, 1614
MluNI TGGCCA 2 cut(s) 1587, 1614
MlyI GAGTC 3 cut(s) 527, 1032, 1832
MmeI TCCRAC 1 cut(s) 1240
MnlI CCTC 9 cut(s) 494, 500, 520, 693, 1287, 1873, 2022, 2034, 2182
Mox20I TGGCCA 2 cut(s) 1587, 1614
MroI TCCGGA 1 cut(s) 556
MroXI GAANNNNTTC 2 cut(s) 30, 195
MscI TGGCCA 2 cut(s) 1587, 1614
MseI TTAA 9 cut(s) 53, 177, 203, 209, 356, 609, 663, 1311, 1409
MslI CAYNNNNRTG 1 cut(s) 2226
Msp20I TGGCCA 2 cut(s) 1587, 1614
MspA1I CMGCKG 2 cut(s) 964, 1463
MspI CCGG 4 cut(s) 331, 451, 557, 1121
MspR9I CCNGG 5 cut(s) 1122, 1251, 1382, 1911, 1966
MunI CAATTG 3 cut(s) 1265, 1605, 1710
Mva1269I GAATGC 2 cut(s) 1764, 2217
MvaI CCWGG 4 cut(s) 1251, 1382, 1911, 1966
MvnI CGCG 1 cut(s) 546
MwoI GCNNNNNNNGC 4 cut(s) 451, 817, 1280, 2244
NciI CCSGG 1 cut(s) 1122
NdeII GATC 8 cut(s) 336, 1218, 1417, 1558, 1568, 1669, 2092, 2275
NheI GCTAGC 1 cut(s) 2240
NlaIV GGNNCC 6 cut(s) 373, 374, 1101, 1797, 1970, 1971
NmuCI GTSAC 2 cut(s) 125, 1116
NspI RCATGY 3 cut(s) 732, 1528, 1887
NspV TTCGAA 1 cut(s) 919
PaeI GCATGC 2 cut(s) 732, 1528
PaeR7I CTCGAG 1 cut(s) 437
PagI TCATGA 2 cut(s) 1192, 1390
PciI ACATGT 1 cut(s) 1883
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PctI GAATGC 2 cut(s) 1764, 2217
PdmI GAANNNNTTC 2 cut(s) 30, 195
PfeI GAWTC 4 cut(s) 1325, 1397, 1552, 1806
PflFI GACNNNGTC 1 cut(s) 1114
PflMI CCANNNNNTGG 2 cut(s) 1594, 1610
PkrI GCNGC 4 cut(s) 966, 969, 1602, 2246
PleI GAGTC 3 cut(s) 526, 1031, 1831
PpsI GAGTC 3 cut(s) 526, 1031, 1831
PpuMI RGGWCCY 2 cut(s) 372, 1969
PscI ACATGT 1 cut(s) 1883
PsiI TTATAA 2 cut(s) 1095, 1752
Psp5II RGGWCCY 2 cut(s) 372, 1969
Psp6I CCWGG 4 cut(s) 1249, 1380, 1909, 1964
PspEI GGTNACC 1 cut(s) 1116
PspGI CCWGG 4 cut(s) 1249, 1380, 1909, 1964
PspN4I GGNNCC 6 cut(s) 373, 374, 1101, 1797, 1970, 1971
PspPI GGNCC 5 cut(s) 372, 1100, 1457, 1969, 2251
PspPPI RGGWCCY 2 cut(s) 372, 1969
PstNI CAGNNNCTG 1 cut(s) 842
PsyI GACNNNGTC 1 cut(s) 1114
PvuII CAGCTG 1 cut(s) 1463
RsaI GTAC 4 cut(s) 243, 250, 1467, 2167
RsaNI GTAC 4 cut(s) 242, 249, 1466, 2166
RseI CAYNNNNRTG 1 cut(s) 2226
SaqAI TTAA 9 cut(s) 53, 177, 203, 209, 356, 609, 663, 1311, 1409
SatI GCNGC 4 cut(s) 965, 968, 1601, 2245
Sau3AI GATC 8 cut(s) 336, 1218, 1417, 1558, 1568, 1669, 2092, 2275
Sau96I GGNCC 5 cut(s) 372, 1100, 1457, 1969, 2251
SchI GAGTC 3 cut(s) 527, 1032, 1832
ScrFI CCNGG 5 cut(s) 1122, 1251, 1382, 1911, 1966
SduI GDGCHC 3 cut(s) 1245, 1881, 2101
SexAI ACCWGGT 1 cut(s) 1380
SfaNI GCATC 4 cut(s) 1351, 1636, 1666, 1868
SfcI CTRYAG 1 cut(s) 2106
Sfr274I CTCGAG 1 cut(s) 437
SfuI TTCGAA 1 cut(s) 919
SinI GGWCC 3 cut(s) 372, 1969, 2251
SlaI CTCGAG 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 2226
SmlI CTYRAG 2 cut(s) 437, 2126
SmoI CTYRAG 2 cut(s) 437, 2126
SphI GCATGC 2 cut(s) 732, 1528
SsiI CCGC 5 cut(s) 363, 546, 964, 967, 2025
SspI AATATT 3 cut(s) 196, 751, 1141
SspMI CTAG 5 cut(s) 1497, 1566, 1820, 2067, 2241
StyD4I CCNGG 5 cut(s) 1120, 1249, 1380, 1909, 1964
TaaI ACNGT 4 cut(s) 248, 621, 2003, 2075
TaiI ACGT 1 cut(s) 392
TaqI TCGA 6 cut(s) 40, 345, 438, 696, 919, 2278
TaqII GACCGA 1 cut(s) 887
TatI WGTACW 2 cut(s) 241, 2165
TauI GCSGC 2 cut(s) 967, 970
TfiI GAWTC 4 cut(s) 1325, 1397, 1552, 1806
Tru1I TTAA 9 cut(s) 53, 177, 203, 209, 356, 609, 663, 1311, 1409
Tru9I TTAA 9 cut(s) 53, 177, 203, 209, 356, 609, 663, 1311, 1409
TscAI CASTG 6 cut(s) 130, 592, 856, 1011, 1018, 2204
TseFI GTSAC 2 cut(s) 125, 1116
TseI GCWGC 2 cut(s) 1600, 2244
Tsp45I GTSAC 2 cut(s) 125, 1116
TspGWI ACGGA 4 cut(s) 628, 1318, 1726, 1808
TspRI CASTG 6 cut(s) 130, 592, 856, 1011, 1018, 2204
Tth111I GACNNNGTC 1 cut(s) 1114
Van91I CCANNNNNTGG 2 cut(s) 1594, 1610
VpaK11BI GGWCC 3 cut(s) 372, 1969, 2251
XagI CCTNNNNNAGG 1 cut(s) 1908
XapI RAATTY 6 cut(s) 26, 118, 692, 845, 1307, 1732
XceI RCATGY 3 cut(s) 732, 1528, 1887
XcmI CCANNNNNNNNNTGG 1 cut(s) 1408
XhoI CTCGAG 1 cut(s) 437
XmiI GTMKAC 2 cut(s) 235, 1200
XmnI GAANNNNTTC 2 cut(s) 30, 195
XspI CTAG 5 cut(s) 1497, 1566, 1820, 2067, 2241
ZraI GACGTC 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.