MD10G1232100.v1.1

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003630
Physical Location & Seq
Forward (+)
32839538 .. 32842673
3136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1232100.v1.1.491

Sequence Viewer

Length: 2916 bp
ATGGTTGCTATGAATAGTGAATTTGGCAGCCTTTTGCCAACAACAACAATGGAGTCAGACGAGGGGCAGGATTCTTTTCGTGGAGTATATCCTGAATACGGTGCAATTTTCATGTCGAGCAGTGCAATGAAAGATGAATGTTTTAAACAGGGACTGTTCGGCCTTCCATCTTCACAAGGCCAATTTTTAAAGCAGGTTAAAACTGGAATGATTCTATTTCTGTTTGAATACGAGAGGAGAGAACTTCATGGTGTTTGTCAAGCATGCTCTGATGGTGAAATGAATATTTTGCCTTCTGCATACAATTCATCAGGAAAGCAGTTTCCTGCTCAGGTAAAAGTCAAGCCAATTTGGCATTGCCATCCGCTGTCAGAACCTGAATTCAGTGGCGCGATCGAAGATAACTACTTTTCAAATTGGAAGTTCCATTTCGGTCTCTCCAAAGCTCAGGTCCGAAGGCTTTTAATGTTGTTCAGTTCACGAAAGTTAAAATATCAGCCGCGGCAGAGAGAATTATCCAGTAAAAGAGAACCAATATCAGTAGACACTGCTGGTAGAGTCAAGGAAGTCGATGATGGCAGGTCTGTATTGAGTGATAACGCAAACAACGAGCTTGATGCTGACAATCATAATGGGCCAGCAATGACTCAGTCACCGGGGAATGTCTATGAGAATAATGGAAAAGAAGATGGTGGCATGTTGGCAAGTCATAAAGTGGGTGATCTTCATAAAGTAGACAATAGTGAAGGTAATGTTTTTGGGCCAGCTGGACCAACTGAGAACCCTGCTTTCCTTCAGTCTAGTCTTGATAGACCTGTCTGTCCTGGCATGCCTGTTGAAGAAACAGGTTCTTTGATTCAAGATCAAACTAAATCAACCTCAACTGTTGGCCTGCAAATCACTGGCCATTCTTGTTCAGCATCTCCATCTTATAGAGATGCAATTGTCCCCGCCACTCTTCCCTATGATCCTGATGCTTTTAGTTTGAACAATCCACTCTCACCATCAATTGGGGTCAATCAGAGTTCAAATTCCGTTCAAGATTGTTATAAACAACATGGCATTCCTTCCATTACAAATCAATCATATACAGAGTCAAAAGGAATCAATCAAAGCCTAGAATCCACTTCAAAGTTTGATGTTCATGTTCCAATTGCATCCCCTGACCATTACGAGCACTCATGTCAGGGGAACATCGTGCATTTTCCAGAGGTGGCATATTCTGAAGAAATGGCTATAGATTCTTCTGGAAAACAGTGTTCCCGGGAGCCAGTTTTGAAGAATTCTTTATCATCTGAAAGTTTATCACAAATTGGAAGTAGTGGGAGGGAAACCAAGAGCCCTTCATCTTATTCTCTCAGTCCTAGCAACTGTCTGTCCTTTGTGATTGATCGTGCTCATCATATAGCATTACAAGAAAAACTTGAGCAAGAAATGGCACAGAAATTGAATGATGAACCATGTATTGCTTATGTAACTTCATCAGATGAGCTTCAGTGGCAGTTGCAATGTAGTGTTCATCCTGATGATCATAGCAGTGTGGACCACCGTCATCAGAAATCTTTTGATCATGATCTCAAGTCTTATGCATGTTCTGAAGGAATGTGTTCTGATCTGAAGAATCAAAGAAGTGTATTTTCTCGCTTGACTTCAGATACGCCTGGAAAAGAGAATAGTACGCATGCTGACTACGAGGAAACTGATAATGATGCATCAGTGGATGAAGTCATGGCAATGTTGAGTGCGAGTAACTACAGCTGGGTGAAGTCAAAAAAATCTAAACGACCTACTAGACGGCATGGTGACGAGAAGTTTATGAGTAATAAAAAGACCAAAGTGGACTCTGAGTTGGGTAGTAATGACTTGGAGTTGATTCCAAAGAAGTCAAACATGGCTTCTGCTACATGCGTTGAAGATAAAGTTGATCAAAGGCGTGAAGAGATACCCTTTGTGGATTTCAAGAGGCGGACTGAACTGCGGAAACGTAATGGTGATACCAAAGCTAGAGCGAGGGATGAAATTGTAAGAAAAGATAGACTGTTGGGTGGGCAATGCAAGAGAAGGAAGTTGATCAGGCCGAAGTTCAATGACAATGAGCCACGCAATGAAAAAAGCATAAATGGAAACATATGTCTGAATTTACAAGCATCATCACAAGAATCTTCTGTTAGTGAAGATGTTAATGGAAAGAGAAGGAAGCTGGTTAGACCAAAGTTCAGGGTGAATGAGCCATGTGATGACAAAAGCATGAATGGAAACACGTCTAAGATTTTACAAGTGTCATCCCAAAAATGTTCTACTAGTGAAGAAAGTGGAAGTTGCGAGGCTTCTATTGGAAGCCAAGGCAAGTTGTTACCGCAAGGTACAGATTTGTCGCTTGTGGTGTGTCAGAGCAGTCATGAGAATGAGAATAATCAAACTGAAAGATCTTCAAAATATGAAAGAGAAATTGAAGTTGAAAGCTCTGCAGCATCAGTCAACTTTGGAGATGAAGGTCGAAAAGAGCCTCTTTGTGATGTTGGCAGCCAAATTGCTGACCCTGCAATCCCTAAGTTGTTAGTGCAATGTACAGAATCATCTCAAGTGGTTCATGAAGGCCTAAAAGAGCTTCTTCTTGACGTTGGTAGCCAAATTGCTAACCTTGCAATTCCTAAATTGTTACCACAATGTAAAGAATCACCTCAAGTGGTTCATCAAACCAGTCGTGACAATGAGAATATTGAAATGGAAAGATTTCCAGAATATGAGCAGGAAATTAAAACTGAAATTTCCGGGCCATCTCTCAAAGTTGGAGATGAAGGTGGAATGGAGCCTCTTCATGATCTTGGCAGCCTTGCAATCCCATCAACAACAACAACAACAACAACAACAACAAAGCCTTTTCCCACCAAGTGGGGTCGGCTATATGAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

972

Amino Acids

107.52

Weight (kDa)

5.54

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 34 - 158 1.5e-44 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1050
AasI GACNNNNNNGTC 1 cut(s) 819
Acc36I ACCTGC 2 cut(s) 184, 570
AccB7I CCANNNNNTGG 2 cut(s) 1010, 2892
AccI GTMKAC 2 cut(s) 543, 735
AccII CGCG 2 cut(s) 392, 502
AciI CCGC 7 cut(s) 365, 500, 502, 951, 1966, 1978, 2357
AclWI GGATC 1 cut(s) 962
AcoI YGGCCR 1 cut(s) 904
AcsI RAATTY 6 cut(s) 20, 380, 1030, 1282, 2137, 2766
AcuI CTGAAG 6 cut(s) 779, 1245, 1478, 1617, 1635, 1637
AdeI CACNNNGTG 1 cut(s) 2892
AfaI GTAC 3 cut(s) 1678, 2365, 2569
AfiI CCNNNNNNNGG 3 cut(s) 98, 1010, 2892
AflIII ACRYGT 1 cut(s) 2259
AhlI ACTAGT 1 cut(s) 2300
AjiI CACGTC 1 cut(s) 2262
AjnI CCWGG 2 cut(s) 823, 1660
AluBI AGCT 9 cut(s) 446, 613, 767, 1492, 1758, 2003, 2200, 2463, 2608
AluI AGCT 9 cut(s) 446, 613, 767, 1492, 1758, 2003, 2200, 2463, 2608
Alw21I GWGCWC 2 cut(s) 1179, 1399
Alw26I GTCTC 1 cut(s) 440
AlwI GGATC 1 cut(s) 962
AlwNI CAGNNNCTG 2 cut(s) 154, 377
Ama87I CYCGRG 1 cut(s) 1263
AoxI GGCC 9 cut(s) 160, 178, 635, 761, 889, 904, 2075, 2596, 2774
ApeKI GCWGC 4 cut(s) 27, 2468, 2523, 2829
ApoI RAATTY 6 cut(s) 20, 380, 1030, 1282, 2137, 2766
ArsI GACNNNNNNTTYG 2 cut(s) 435, 467
Asp700I GAANNNNTTC 2 cut(s) 1606, 2733
AspLEI GCGC 1 cut(s) 392
AspS9I GGNCC 6 cut(s) 451, 635, 761, 770, 1543, 2774
AsuC2I CCSGG 4 cut(s) 657, 1264, 1265, 2773
AsuHPI GGTGA 9 cut(s) 287, 645, 731, 993, 1774, 1814, 2003, 2233, 2670
AvaI CYCGRG 1 cut(s) 1263
AvaII GGWCC 3 cut(s) 451, 770, 1543
BalI TGGCCA 1 cut(s) 906
BanII GRGCYC 1 cut(s) 1343
Bbv12I GWGCWC 2 cut(s) 1179, 1399
BbvI GCAGC 4 cut(s) 39, 2480, 2535, 2841
BccI CCATC 9 cut(s) 175, 266, 369, 569, 683, 934, 1012, 2785, 2851
BceAI ACGGC 1 cut(s) 1811
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 2 cut(s) 825, 1662
BclI TGATCA 4 cut(s) 1528, 1567, 1924, 2070
BcnI CCSGG 4 cut(s) 657, 1264, 1265, 2773
BcoDI GTCTC 1 cut(s) 440
BcuI ACTAGT 1 cut(s) 2300
BfaI CTAG 7 cut(s) 801, 1118, 1365, 1791, 2004, 2301, 2914
BfmI CTRYAG 3 cut(s) 1236, 1753, 2466
BfuAI ACCTGC 2 cut(s) 184, 570
BglI GCCNNNNNGGC 1 cut(s) 352
BglII AGATCT 1 cut(s) 2426
BisI GCNGC 6 cut(s) 28, 500, 503, 2469, 2524, 2830
BlsI GCNGC 6 cut(s) 29, 501, 504, 2470, 2525, 2831
Bme1390I CCNGG 6 cut(s) 657, 825, 1264, 1265, 1662, 2773
Bme18I GGWCC 3 cut(s) 451, 770, 1543
BmeT110I CYCGRG 1 cut(s) 1263
BmgBI CACGTC 1 cut(s) 2262
BmgT120I GGNCC 6 cut(s) 451, 635, 761, 770, 1543, 2774
BmiI GGNNCC 2 cut(s) 1269, 2811
BmrFI CCNGG 6 cut(s) 657, 825, 1264, 1265, 1662, 2773
BmsI GCATC 9 cut(s) 607, 928, 929, 964, 1166, 1699, 1721, 2156, 2480
BoxI GACNNNNGTC 1 cut(s) 1548
Bpu10I CCTNAGC 2 cut(s) 330, 447
BpuEI CTTGAG 4 cut(s) 1445, 1562, 2565, 2667
BpuMI CCSGG 4 cut(s) 657, 1264, 1265, 2773
BsaBI GATNNNNATC 1 cut(s) 1572
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 4 cut(s) 500, 656, 1263, 2341
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 1010, 2892
Bse1I ACTGG 5 cut(s) 208, 519, 907, 1271, 2700
Bse3DI GCAATG 8 cut(s) 132, 355, 648, 1514, 1740, 2057, 2110, 2570
Bse8I GATNNNNATC 1 cut(s) 1572
BseBI CCWGG 2 cut(s) 825, 1662
BseDI CCNNGG 4 cut(s) 500, 656, 1263, 2341
BseGI GGATG 6 cut(s) 361, 1157, 1519, 1726, 2020, 2282
BseJI GATNNNNATC 1 cut(s) 1572
BseLI CCNNNNNNNGG 3 cut(s) 98, 1010, 2892
BseMI GCAATG 8 cut(s) 132, 355, 648, 1514, 1740, 2057, 2110, 2570
BseMII CTCAG 6 cut(s) 344, 461, 662, 768, 1372, 1836
BseNI ACTGG 5 cut(s) 208, 519, 907, 1271, 2700
BseRI GAGGAG 1 cut(s) 250
BseXI GCAGC 4 cut(s) 39, 2480, 2535, 2841
BseYI CCCAGC 1 cut(s) 1758
Bsh1236I CGCG 2 cut(s) 392, 502
Bsh1285I CGRYCG 1 cut(s) 396
BshFI GGCC 9 cut(s) 162, 180, 637, 763, 891, 906, 2077, 2598, 2776
BsiEI CGRYCG 1 cut(s) 396
BsiHKAI GWGCWC 2 cut(s) 1179, 1399
BsiHKCI CYCGRG 1 cut(s) 1263
BsiSI CCGG 3 cut(s) 656, 1264, 2772
BslFI GGGAC 2 cut(s) 165, 932
BslI CCNNNNNNNGG 3 cut(s) 98, 1010, 2892
BsmAI GTCTC 1 cut(s) 440
BsmFI GGGAC 2 cut(s) 165, 932
BsmI GAATGC 1 cut(s) 1062
BsnI GGCC 9 cut(s) 162, 180, 637, 763, 891, 906, 2077, 2598, 2776
Bso31I GGTCTC 1 cut(s) 440
BsoBI CYCGRG 1 cut(s) 1263
Bsp1286I GDGCHC 3 cut(s) 1179, 1343, 1399
Bsp1407I TGTACA 1 cut(s) 2567
BspACI CCGC 7 cut(s) 365, 500, 502, 951, 1966, 1978, 2357
BspANI GGCC 9 cut(s) 162, 180, 637, 763, 891, 906, 2077, 2598, 2776
BspCNI CTCAG 6 cut(s) 343, 460, 661, 769, 1371, 1837
BspFNI CGCG 2 cut(s) 392, 502
BspHI TCATGA 4 cut(s) 1570, 2398, 2590, 2818
BspLI GGNNCC 2 cut(s) 1269, 2811
BspMAI CTGCAG 1 cut(s) 2470
BspMI ACCTGC 2 cut(s) 184, 570
BspPI GGATC 1 cut(s) 962
BspTNI GGTCTC 1 cut(s) 440
BsrDI GCAATG 8 cut(s) 132, 355, 648, 1514, 1740, 2057, 2110, 2570
BsrGI TGTACA 1 cut(s) 2567
BsrI ACTGG 5 cut(s) 208, 519, 907, 1271, 2700
BssECI CCNNGG 4 cut(s) 500, 656, 1263, 2341
BssT1I CCWWGG 1 cut(s) 2341
Bst2UI CCWGG 2 cut(s) 825, 1662
Bst4CI ACNGT 7 cut(s) 101, 156, 886, 1257, 1373, 1550, 2040
Bst6I CTCTTC 3 cut(s) 963, 1932, 2820
BstAUI TGTACA 1 cut(s) 2567
BstC8I GCNNGC 6 cut(s) 265, 639, 765, 830, 893, 1683
BstDEI CTNAG 8 cut(s) 330, 447, 648, 777, 1358, 1845, 2265, 2550
BstDSI CCRYGG 1 cut(s) 500
BstF5I GGATG 6 cut(s) 361, 1157, 1519, 1726, 2020, 2282
BstFNI CGCG 2 cut(s) 392, 502
BstHHI GCGC 1 cut(s) 392
BstMAI GTCTC 1 cut(s) 440
BstMCI CGRYCG 1 cut(s) 396
BstMWI GCNNNNNNNGC 4 cut(s) 352, 1498, 2540, 2642
BstNI CCWGG 2 cut(s) 825, 1662
BstNSI RCATGY 6 cut(s) 267, 700, 832, 1593, 1685, 1908
BstPAI GACNNNNGTC 1 cut(s) 1548
BstSCI CCNGG 6 cut(s) 655, 823, 1262, 1263, 1660, 2771
BstSFI CTRYAG 3 cut(s) 1236, 1753, 2466
BstUI CGCG 2 cut(s) 392, 502
BstV1I GCAGC 4 cut(s) 39, 2480, 2535, 2841
BstX2I RGATCY 1 cut(s) 2426
BstYI RGATCY 1 cut(s) 2426
BsuRI GGCC 9 cut(s) 162, 180, 637, 763, 891, 906, 2077, 2598, 2776
BtgI CCRYGG 1 cut(s) 500
BtrI CACGTC 1 cut(s) 2262
BtsCI GGATG 6 cut(s) 361, 1157, 1519, 1726, 2020, 2282
BtsI GCAGTG 3 cut(s) 127, 546, 1543
BtsIMutI CAGTG 8 cut(s) 127, 391, 546, 900, 1262, 1502, 1543, 1722
BveI ACCTGC 2 cut(s) 184, 570
Cac8I GCNNGC 6 cut(s) 265, 639, 765, 830, 893, 1683
CaiI CAGNNNCTG 2 cut(s) 154, 377
CciI TCATGA 4 cut(s) 1570, 2398, 2590, 2818
CfoI GCGC 1 cut(s) 392
Cfr13I GGNCC 6 cut(s) 451, 635, 761, 770, 1543, 2774
Cfr42I CCGCGG 1 cut(s) 503
Cfr9I CCCGGG 1 cut(s) 1263
Csp6I GTAC 3 cut(s) 1677, 2364, 2568
CviQI GTAC 3 cut(s) 1677, 2364, 2568
DdeI CTNAG 8 cut(s) 330, 447, 648, 777, 1358, 1845, 2265, 2550
DraI TTTAAA 2 cut(s) 145, 189
DraIII CACNNNGTG 1 cut(s) 2892
DrdI GACNNNNNNGTC 1 cut(s) 819
DseDI GACNNNNNNGTC 1 cut(s) 819
EaeI YGGCCR 1 cut(s) 904
Eam1104I CTCTTC 3 cut(s) 963, 1932, 2820
EarI CTCTTC 3 cut(s) 963, 1932, 2820
EciI GGCGGA 1 cut(s) 1981
Eco130I CCWWGG 1 cut(s) 2341
Eco147I AGGCCT 1 cut(s) 2598
Eco24I GRGCYC 1 cut(s) 1343
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 3 cut(s) 451, 770, 1543
Eco57I CTGAAG 6 cut(s) 779, 1245, 1478, 1617, 1635, 1637
Eco88I CYCGRG 1 cut(s) 1263
EcoRI GAATTC 2 cut(s) 380, 1282
EcoRII CCWGG 2 cut(s) 823, 1660
EcoT14I CCWWGG 1 cut(s) 2341
EcoT22I ATGCAT 2 cut(s) 1591, 1714
EcoT38I GRGCYC 1 cut(s) 1343
ErhI CCWWGG 1 cut(s) 2341
FalI AAGNNNNNCTT 6 cut(s) 1407, 1439, 2493, 2525, 2595, 2627
FaqI GGGAC 2 cut(s) 165, 932
FauI CCCGC 1 cut(s) 958
FauNDI CATATG 1 cut(s) 2129
FbaI TGATCA 4 cut(s) 1528, 1567, 1924, 2070
FblI GTMKAC 2 cut(s) 543, 735
Fnu4HI GCNGC 6 cut(s) 28, 500, 503, 2469, 2524, 2830
FokI GGATG 6 cut(s) 348, 1144, 1506, 1733, 2027, 2269
FriOI GRGCYC 1 cut(s) 1343
Fsp4HI GCNGC 6 cut(s) 28, 500, 503, 2469, 2524, 2830
FspBI CTAG 7 cut(s) 801, 1118, 1365, 1791, 2004, 2301, 2914
GlaI GCGC 1 cut(s) 391
GluI GCNGC 6 cut(s) 28, 500, 503, 2469, 2524, 2830
GsaI CCCAGC 1 cut(s) 1762
HaeIII GGCC 9 cut(s) 162, 180, 637, 763, 891, 906, 2077, 2598, 2776
HapII CCGG 3 cut(s) 656, 1264, 2772
HhaI GCGC 1 cut(s) 392
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HincII GTYRAC 1 cut(s) 2479
HindII GTYRAC 1 cut(s) 2479
HpaII CCGG 3 cut(s) 656, 1264, 2772
HphI GGTGA 9 cut(s) 287, 645, 731, 993, 1774, 1814, 2003, 2233, 2670
Hpy166II GTNNAC 6 cut(s) 479, 544, 736, 1543, 1840, 2479
Hpy8I GTNNAC 6 cut(s) 479, 544, 736, 1543, 1840, 2479
HpyCH4III ACNGT 7 cut(s) 101, 156, 886, 1257, 1373, 1550, 2040
HpyCH4IV ACGT 3 cut(s) 1984, 2261, 2619
HpyF10VI GCNNNNNNNGC 4 cut(s) 352, 1498, 2540, 2642
HpyF3I CTNAG 8 cut(s) 330, 447, 648, 777, 1358, 1845, 2265, 2550
HpySE526I ACGT 3 cut(s) 1984, 2261, 2619
HspAI GCGC 1 cut(s) 390
Ksp22I TGATCA 4 cut(s) 1528, 1567, 1924, 2070
KspI CCGCGG 1 cut(s) 503
LmnI GCTCC 2 cut(s) 1267, 2809
Lsp1109I GCAGC 4 cut(s) 39, 2480, 2535, 2841
LweI GCATC 9 cut(s) 607, 928, 929, 964, 1166, 1699, 1721, 2156, 2480
MaeI CTAG 7 cut(s) 801, 1118, 1365, 1791, 2004, 2301, 2914
MaeII ACGT 3 cut(s) 1984, 2261, 2619
MaeIII GTNAC 7 cut(s) 651, 1474, 1748, 1802, 2352, 2658, 2705
MfeI CAATTG 3 cut(s) 942, 1008, 1152
MflI RGATCY 1 cut(s) 2426
MhlI GDGCHC 3 cut(s) 1179, 1343, 1399
MlsI TGGCCA 1 cut(s) 906
MluNI TGGCCA 1 cut(s) 906
MlyI GAGTC 5 cut(s) 62, 567, 640, 1103, 1835
MmeI TCCRAC 1 cut(s) 2770
Mox20I TGGCCA 1 cut(s) 906
Mph1103I ATGCAT 2 cut(s) 1591, 1714
MroXI GAANNNNTTC 2 cut(s) 1606, 2733
MscI TGGCCA 1 cut(s) 906
MseI TTAA 7 cut(s) 144, 188, 198, 464, 488, 2181, 2757
MslI CAYNNNNRTG 4 cut(s) 1524, 1536, 1733, 2403
Msp20I TGGCCA 1 cut(s) 906
MspA1I CMGCKG 4 cut(s) 367, 502, 767, 1758
MspI CCGG 3 cut(s) 656, 1264, 2772
MspR9I CCNGG 6 cut(s) 657, 825, 1264, 1265, 1662, 2773
MunI CAATTG 3 cut(s) 942, 1008, 1152
Mva1269I GAATGC 1 cut(s) 1062
MvaI CCWGG 2 cut(s) 825, 1662
MvnI CGCG 2 cut(s) 392, 502
MwoI GCNNNNNNNGC 4 cut(s) 352, 1498, 2540, 2642
NciI CCSGG 4 cut(s) 657, 1264, 1265, 2773
NdeI CATATG 1 cut(s) 2129
NlaIV GGNNCC 2 cut(s) 1269, 2811
NmuCI GTSAC 3 cut(s) 651, 1802, 2705
NsiI ATGCAT 2 cut(s) 1591, 1714
NspI RCATGY 6 cut(s) 267, 700, 832, 1593, 1685, 1908
PaeI GCATGC 3 cut(s) 267, 832, 1685
PagI TCATGA 4 cut(s) 1570, 2398, 2590, 2818
PceI AGGCCT 1 cut(s) 2598
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PctI GAATGC 1 cut(s) 1062
PdmI GAANNNNTTC 2 cut(s) 1606, 2733
PflFI GACNNNGTC 1 cut(s) 649
PflMI CCANNNNNTGG 2 cut(s) 1010, 2892
PkrI GCNGC 6 cut(s) 29, 501, 504, 2470, 2525, 2831
Ple19I CGATCG 1 cut(s) 396
PleI GAGTC 5 cut(s) 61, 566, 640, 1102, 1835
PpsI GAGTC 5 cut(s) 61, 566, 640, 1102, 1835
PshAI GACNNNNGTC 1 cut(s) 1548
PsiI TTATAA 1 cut(s) 1050
Psp6I CCWGG 2 cut(s) 823, 1660
PspFI CCCAGC 1 cut(s) 1758
PspGI CCWGG 2 cut(s) 823, 1660
PspN4I GGNNCC 2 cut(s) 1269, 2811
PspPI GGNCC 6 cut(s) 451, 635, 761, 770, 1543, 2774
PstI CTGCAG 1 cut(s) 2470
PstNI CAGNNNCTG 2 cut(s) 154, 377
PsuI RGATCY 1 cut(s) 2426
PsyI GACNNNGTC 1 cut(s) 649
PvuI CGATCG 1 cut(s) 396
PvuII CAGCTG 2 cut(s) 767, 1758
RsaI GTAC 3 cut(s) 1678, 2365, 2569
RsaNI GTAC 3 cut(s) 1677, 2364, 2568
RseI CAYNNNNRTG 4 cut(s) 1524, 1536, 1733, 2403
SacII CCGCGG 1 cut(s) 503
SaqAI TTAA 7 cut(s) 144, 188, 198, 464, 488, 2181, 2757
SatI GCNGC 6 cut(s) 28, 500, 503, 2469, 2524, 2830
Sau96I GGNCC 6 cut(s) 451, 635, 761, 770, 1543, 2774
SchI GAGTC 5 cut(s) 62, 567, 640, 1103, 1835
ScrFI CCNGG 6 cut(s) 657, 825, 1264, 1265, 1662, 2773
SduI GDGCHC 3 cut(s) 1179, 1343, 1399
SfaNI GCATC 9 cut(s) 607, 928, 929, 964, 1166, 1699, 1721, 2156, 2480
SfcI CTRYAG 3 cut(s) 1236, 1753, 2466
Sfr303I CCGCGG 1 cut(s) 503
SgrBI CCGCGG 1 cut(s) 503
SinI GGWCC 3 cut(s) 451, 770, 1543
SmaI CCCGGG 1 cut(s) 1265
SmiMI CAYNNNNRTG 4 cut(s) 1524, 1536, 1733, 2403
SmlI CTYRAG 4 cut(s) 1424, 1577, 2580, 2682
SmoI CTYRAG 4 cut(s) 1424, 1577, 2580, 2682
SpeI ACTAGT 1 cut(s) 2300
SphI GCATGC 3 cut(s) 267, 832, 1685
SseBI AGGCCT 1 cut(s) 2598
SsiI CCGC 7 cut(s) 365, 500, 502, 951, 1966, 1978, 2357
SspI AATATT 2 cut(s) 286, 2719
SspMI CTAG 7 cut(s) 801, 1118, 1365, 1791, 2004, 2301, 2914
StuI AGGCCT 1 cut(s) 2598
StyD4I CCNGG 6 cut(s) 655, 823, 1262, 1263, 1660, 2771
StyI CCWWGG 1 cut(s) 2341
TaaI ACNGT 7 cut(s) 101, 156, 886, 1257, 1373, 1550, 2040
TaiI ACGT 3 cut(s) 1987, 2264, 2622
TaqI TCGA 4 cut(s) 116, 396, 570, 2497
TaqII GACCGA 1 cut(s) 422
TatI WGTACW 1 cut(s) 2567
TauI GCSGC 2 cut(s) 502, 505
Tru1I TTAA 7 cut(s) 144, 188, 198, 464, 488, 2181, 2757
Tru9I TTAA 7 cut(s) 144, 188, 198, 464, 488, 2181, 2757
TscAI CASTG 8 cut(s) 127, 391, 553, 907, 1262, 1502, 1543, 1722
TseFI GTSAC 3 cut(s) 651, 1802, 2705
TseI GCWGC 4 cut(s) 27, 2468, 2523, 2829
Tsp45I GTSAC 3 cut(s) 651, 1802, 2705
TspGWI ACGGA 1 cut(s) 1024
TspMI CCCGGG 1 cut(s) 1263
TspRI CASTG 8 cut(s) 127, 391, 553, 907, 1262, 1502, 1543, 1722
Tth111I GACNNNGTC 1 cut(s) 649
Van91I CCANNNNNTGG 2 cut(s) 1010, 2892
VpaK11BI GGWCC 3 cut(s) 451, 770, 1543
XapI RAATTY 6 cut(s) 20, 380, 1030, 1282, 2137, 2766
XceI RCATGY 6 cut(s) 267, 700, 832, 1593, 1685, 1908
XmaI CCCGGG 1 cut(s) 1263
XmiI GTMKAC 2 cut(s) 543, 735
XmnI GAANNNNTTC 2 cut(s) 1606, 2733
XspI CTAG 7 cut(s) 801, 1118, 1365, 1791, 2004, 2301, 2914
Zsp2I ATGCAT 2 cut(s) 1591, 1714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.