Rw5G013000

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003630
Physical Location & Seq
Reverse (-)
15351087 .. 15356334
5248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013000.1

Sequence Viewer

Length: 3699 bp
ATGGAGTCAGATGAGGAGCACAGGACTGTTCAAGGAATGTCTCCCGAATATGGTGCAATTTTCATGTCAAGCAGTTTTACAAAAGATGAATGTTTTAGACGAAGACTGTTTGGCCTTCCATCTGGACAAGGCCCATTTGTAAAGCAGATCAAAAGTGGAATGATTTTATTTCTCTTTGAATTTGAGAAGAGAGAACTCCATGGTGTATTCCAGGCATCCTCTGATGGTAGAATGAATATTGTTCCTAATGCATGGAGTAGATCAGGAAAGAAGTTTCCTGCTCAGGTGAGAGTCAACCCAATTTGGCATTGTCATCCGCTTCCAGAATCTGAGTTTTGTGACGCAATCAAAGAAAACTATTTTTCAAAATGGAAATTCAATTTTGGTCTTTCCAAAGCTCAGGTTTGGAGGCTTCTAATGTTGTTTAATTTGCGAAAATTGAAAAGCCCACATCCTCAGAGAGCACTAGCTAGAAATACTGCTGCACAACCAGCGGACATTTATCGAGAACTTGATGATGGCAGATTATCAAGTGATAAAGTGGGTAATCTACATAAAGTGGACAATACGCTTCGGTCGATCATCCAGAGCAGGTATCTTGAACACCTGCCCGGCAAAGTTGAAAGGGATGATGCTAAGTTTGGGAATGGAGTTAATGTGGATTTTGAACACAGGGCATTGCCATCAACTGAGCATACTAGGTCAGGCCAAAATGGAAGATGTGATGGCAGCCTGGAAATGTGTGGAAGGGAAGGAAGTGTCAGTCACTTGGATGGTCCTTTTTATTCTGGAATGCCTCTTCAAGAAACATATTCTTTGGTTCAAGATAAAGAAACTTCTAGCCATCAGATGGAGCTGCCAATCACTGGCCACTCTTATGCAGCATCTCGTGGAGATGCAATTATTACGAGCATTCTTCCCTATGATCCTGATGATCCTAGATTGAACCTTGCACATTCTCGACTAGTTGGAAATTATGATTCTGTTCAAGACTGTGATGGTCAGAATGGCATTCCCGCGGTGAGGAATGAAGTAAATCCCTCGTACACAGAGACAAATTCACTAAATCAAAGCCTAGAAGACATTTCTAAGGTTGATGTTCGCAGATCACTTTCAGCCATCAATATTACTCCTTTTCCAGGGGCAACATCTTCAGCAGAAATGGCTATTTACTATTCAAAGTCCTCTTTAGCTCCTGAGTCTTTATCAGAATTTGGAAGTAATGGGAAGGACGGGCCTTCCTCTTATCCCCTTAGCCCCAGTGATTATCCTTCCTTTCTGGTTGACCGCAGGCATCCTGTAGCAGTACAACATAAACCAGTGCATGAAATAGGACCATTTACTGCAAATGTAACTTCATTAGAGGAGATACAGTGCCAGTCACCATGCCGCACTCATTCTACTGACCTTGACAGTGTGGACTACCACGACTGTAAATGTTCTGATCATAAAATTTCTGATAGAATGCTGTGTTCAGATCTTCTGGAAAACAGAAGTAGTGTGTTCTCTCGCCTGAGATTCAATAACCCTGGATGGTGTCTGGAAAACCTTGGACATCTTGGCCACGAGGAAGATGTTAAAGACACGTCATCAGTGGATGAAGTCATGGAAATGTTGAGCGAGAGACACTACCCCTGGATGGAGAGTGAAAAATCTAAACTGCATAAGAGACGACAAGGTAATGTAGAGAAGTCTAGGAATTTGAAGCAGACAGCTGTGAAGTCTGAGTTGGAAATGATTCCAGAGAAGGCAAACACAGCTTCTGCTTCACCAACTGGAGATAAAGCTGATCAAAGCAGTAAAAAGACACTGTTTGTGGACTTCAAGCGGCGGAGTGATTTGCGAAAAGTCAATAGCGATACTAATGGGGAAAGTGCTGGAAGCAAGGGATTGTTAGGTGGGCAGTGCAAGAGAAGGAAGTTAATCAGGCCTAACTTCAGTGAGAATGAGCCACGTGATAACAAAAGCTATAATAGAAGTCATTTTATGAATTCACCAGGATCAGCCCAAGAATGCCGTAGTTGTGAAGATGCTGGTGGCAAGAAAAGGAAGCTGGCTAAGCCAAATTTCAGAGAGAACGAGTCATCTCAGGAATCATCTGCGCAATGTTCTATTGGTGAAGATGCTGGTGGCAAGAGAAGGAAGCTGGCTAGGCCAAATTTTAGAGAAAATGAGTCATCTTTAATATCCTCGCCACAATGTTCTGTTGGTGAAGATGCTGGTGGAAGTTGCGGGTCTTTTGTTGGAAGCCAAGGTAAGTTGTTATTACAAGGTGCAGAATCTTCACACGACAATGAGAAGATCGATACTGAATGTGTTTCTAAATATGAAACAGAAATTATAGTAGAAAGTTCTGGGGGTTCTCCCAAAATTGGAGATGATAGTGGAAAGGGGTGTTTGCATGCCTATCCTGGAAATTCTTGTCTGAACTTACAAGCATCAGTGGATGAATCTTCTTTTTGTGAAGATGCTAATTGTAAGGAGGAGCTAGTTGGGCCTGATTTCAGAGAGAATGAGCTACTCCAAGTGTCGCCACATAAATGTTCTAGCGGTGAATATGCTGGTGAAAAGTATGAGGCATTGGTTGGAAGCCAAGGTAACATTGATGCTCAAATATTTCCAACATTTAAAATGGAAATTAAACAATTAAGCTCTGGGGAGTCTCTCAAAATTGGAGATGAAAGTGGAAAGGGTCCTGTGCATGATGTTGGCTGCCAAATTGCCATCGGCCCTTCCAAAGATGAGATTCTTAATGCACAGGAAGACTTGGATCACAAGGACCCTATGGTGTTGTCTCGAGATGGTCATCAGCCCAGTCAGTCCACCATGCTAAACTCAGCTTCGCAACCCAAGACAAATGGATTTTCCGAATTTGAAAGTGACAGAGGCAGTGAACTGGAGATGCTTCCAAGGGTTGAATTTTCTCCAAACATAGTTGGCAATGGTGGCTCCAGTAAGCAGAACCCTGACTCACCAAGAGTAGCTGAAAACATCTCTAATCGCGTGGATGCGGGTATGATAAATTATCAACACGGATCCAGTACTGAGCAGGGTCAGGCATCTTGCAACTCCAACCAGCATGATTCACTTAAACAGGAAGGGTTCGGGAATGATGTGCTGGAGGCTGTGGAGTTTGGTGATAATGTTGCAAGTGATAACACTTCAAGAGAAAGAGAAATAGACATCAAACTAGAAAGCTGTGTGGATTCTGTCAAAAGTGGAGATGAAAATGGAATGGAGAGCGTGCAGGTTGGTATTCTTAGTGCACCGGAAGACTCTATACTTACCAGGTTGTCTGAAGACTTTTCCGAGACCGATCAGATAACTGTGCTAGAGTCAGCTACGCAACCTAAGACGAATTCTGGAACAAGTAATGGATTTTCTGAAATCGAAGGTGACAGAGGCAGTGAACTGCAGGTGGTTCCAATAGTTGAACGTTGTCCAAATATAAACGCCTTTGATAGTGCAGATGCAAGTATGGTAAATAACCAACAAGGAGTCAATTCTGAGCAGTTGCAGACGTCTTGCAACTCCAACAATCACAGATCACTTCAAGAGCATGGATCCAAACATAATGTGCAAGAGAGCATGGAGTTCGGTGATAATGAGGCAATTGATGACACTGTAAGAGAAAACAAGAGGCTATGGAGAATTTTAACAGCTAGATTGCAGCTTGTTAAGAGGGCACAACAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

1232

Amino Acids

136.03

Weight (kDa)

5.32

Isoelectric Point (pI)

54.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 18 - 142 3.2e-48 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 615, 3406
AatII GACGTC 1 cut(s) 3524
Acc16I TGCGCA 1 cut(s) 2103
Acc36I ACCTGC 4 cut(s) 582, 615, 3238, 3406
AccB7I CCANNNNNTGG 2 cut(s) 850, 866
AccII CGCG 2 cut(s) 1019, 3003
AclI AACGTT 1 cut(s) 3436
AclWI GGATC 8 cut(s) 920, 929, 2008, 2778, 3030, 3043, 3558, 3571
AcoI YGGCCR 2 cut(s) 868, 1561
AcuI CTGAAG 3 cut(s) 1137, 1921, 3318
AcvI CACGTG 1 cut(s) 1955
AcyI GRCGYC 1 cut(s) 3521
AfaI GTAC 3 cut(s) 1046, 1308, 3043
AfiI CCNNNNNNNGG 7 cut(s) 50, 850, 866, 1023, 1139, 1639, 2372
AflIII ACRYGT 1 cut(s) 1584
AhlI ACTAGT 1 cut(s) 964
AjiI CACGTC 1 cut(s) 1587
AjnI CCWGG 8 cut(s) 210, 732, 1138, 1528, 1634, 1996, 2410, 3287
AjuI GAANNNNNNNTTGG 2 cut(s) 1712, 1744
Alw21I GWGCWC 3 cut(s) 21, 466, 3268
Alw26I GTCTC 7 cut(s) 45, 1046, 1618, 1663, 2667, 2799, 3305
Alw44I GTGCAC 1 cut(s) 3264
AlwI GGATC 8 cut(s) 920, 929, 2008, 2778, 3030, 3043, 3558, 3571
AlwNI CAGNNNCTG 2 cut(s) 329, 1763
Ama87I CYCGRG 1 cut(s) 2796
ApaLI GTGCAC 1 cut(s) 3264
ApeKI GCWGC 6 cut(s) 482, 729, 856, 881, 2712, 3670
Asp700I GAANNNNTTC 1 cut(s) 1737
AspLEI GCGC 1 cut(s) 2104
AspS9I GGNCC 8 cut(s) 131, 776, 1235, 1334, 2495, 2693, 2729, 2779
AsuC2I CCSGG 1 cut(s) 612
AvaI CYCGRG 1 cut(s) 2796
AvaII GGWCC 4 cut(s) 776, 1334, 2693, 2779
BaeGI GKGCMC 2 cut(s) 3268, 3688
BalI TGGCCA 2 cut(s) 870, 1563
BamHI GGATCC 2 cut(s) 3035, 3563
BarI GAAGNNNNNNTAC 2 cut(s) 3264, 3296
BauI CACGAG 2 cut(s) 888, 1565
BbrPI CACGTG 1 cut(s) 1955
BbsI GAAGAC 5 cut(s) 109, 1086, 2769, 3279, 3306
Bbv12I GWGCWC 3 cut(s) 21, 466, 3268
BbvI GCAGC 6 cut(s) 469, 741, 843, 893, 2699, 3682
BceAI ACGGC 1 cut(s) 2001
BcgI CGANNNNNNTGC 4 cut(s) 35, 69, 3577, 3611
BciT130I CCWGG 8 cut(s) 212, 734, 1140, 1530, 1636, 1998, 2412, 3289
BclI TGATCA 2 cut(s) 1444, 1789
BcnI CCSGG 1 cut(s) 612
BcoDI GTCTC 7 cut(s) 45, 1046, 1618, 1663, 2667, 2799, 3305
BcuI ACTAGT 1 cut(s) 964
BfmI CTRYAG 2 cut(s) 1299, 3413
BfuAI ACCTGC 4 cut(s) 582, 615, 3238, 3406
BglII AGATCT 1 cut(s) 1477
BisI GCNGC 8 cut(s) 483, 730, 857, 882, 1390, 1829, 2713, 3671
BlpI GCTNAGC 1 cut(s) 2058
BlsI GCNGC 8 cut(s) 484, 731, 858, 883, 1391, 1830, 2714, 3672
BmcAI AGTACT 1 cut(s) 3043
Bme1390I CCNGG 9 cut(s) 212, 612, 734, 1140, 1530, 1636, 1998, 2412, 3289
Bme18I GGWCC 4 cut(s) 776, 1334, 2693, 2779
BmeT110I CYCGRG 1 cut(s) 2796
BmgBI CACGTC 1 cut(s) 1587
BmgT120I GGNCC 8 cut(s) 131, 776, 1235, 1334, 2495, 2693, 2729, 2779
BmiI GGNNCC 6 cut(s) 2694, 2781, 2950, 3037, 3423, 3565
BmrFI CCNGG 9 cut(s) 212, 612, 734, 1140, 1530, 1636, 1998, 2412, 3289
BmrI ACTGGG 2 cut(s) 1254, 2808
BmuI ACTGGG 2 cut(s) 1254, 2808
BpiI GAAGAC 5 cut(s) 109, 1086, 2769, 3279, 3306
BpmI CTGGAG 4 cut(s) 1797, 2918, 2935, 3140
Bpu10I CCTNAGC 3 cut(s) 282, 399, 1253
Bpu1102I GCTNAGC 1 cut(s) 2058
BpuMI CCSGG 1 cut(s) 612
Bsa29I ATCGAT 1 cut(s) 2304
BsaAI YACGTR 1 cut(s) 1955
BsaBI GATNNNNATC 1 cut(s) 2805
BsaHI GRCGYC 1 cut(s) 3521
BsaI GGTCTC 1 cut(s) 3305
BsaJI CCNNGG 9 cut(s) 199, 1017, 1139, 1528, 1549, 1634, 2251, 2593, 2909
BsaWI WCCGGW 1 cut(s) 3268
BsaXI ACNNNNNCTCC 4 cut(s) 642, 672, 1358, 1388
Bsc4I CCNNNNNNNGG 7 cut(s) 50, 850, 866, 1023, 1139, 1639, 2372
Bse1I ACTGG 9 cut(s) 871, 1260, 1319, 1378, 1780, 2814, 2901, 2952, 3039
Bse3DI GCAATG 3 cut(s) 677, 2111, 2947
Bse8I GATNNNNATC 1 cut(s) 2805
BseBI CCWGG 8 cut(s) 212, 734, 1140, 1530, 1636, 1998, 2412, 3289
BseCI ATCGAT 1 cut(s) 2304
BseDI CCNNGG 9 cut(s) 199, 1017, 1139, 1528, 1549, 1634, 2251, 2593, 2909
BseJI GATNNNNATC 1 cut(s) 2805
BseLI CCNNNNNNNGG 7 cut(s) 50, 850, 866, 1023, 1139, 1639, 2372
BseMI GCAATG 3 cut(s) 677, 2111, 2947
BseNI ACTGG 9 cut(s) 871, 1260, 1319, 1378, 1780, 2814, 2901, 2952, 3039
BseRI GAGGAG 3 cut(s) 29, 1379, 2498
BseSI GKGCMC 2 cut(s) 3268, 3688
BseXI GCAGC 6 cut(s) 469, 741, 843, 893, 2699, 3682
BsgI GTGCAG 4 cut(s) 468, 2295, 3266, 3486
Bsh1236I CGCG 2 cut(s) 1019, 3003
Bsh1285I CGRYCG 1 cut(s) 578
BshVI ATCGAT 1 cut(s) 2304
BsiEI CGRYCG 1 cut(s) 578
BsiHKAI GWGCWC 3 cut(s) 21, 466, 3268
BsiHKCI CYCGRG 1 cut(s) 2796
BsiSI CCGG 2 cut(s) 612, 3269
BslI CCNNNNNNNGG 7 cut(s) 50, 850, 866, 1023, 1139, 1639, 2372
BsmAI GTCTC 7 cut(s) 45, 1046, 1618, 1663, 2667, 2799, 3305
BsmBI CGTCTC 1 cut(s) 1663
BsmI GAATGC 5 cut(s) 798, 912, 1011, 1470, 2018
Bso31I GGTCTC 1 cut(s) 3305
BsoBI CYCGRG 1 cut(s) 2796
Bsp1286I GDGCHC 4 cut(s) 21, 466, 3268, 3688
Bsp1720I GCTNAGC 1 cut(s) 2058
Bsp19I CCATGG 1 cut(s) 199
BspDI ATCGAT 1 cut(s) 2304
BspFNI CGCG 2 cut(s) 1019, 3003
BspLI GGNNCC 6 cut(s) 2694, 2781, 2950, 3037, 3423, 3565
BspMAI CTGCAG 1 cut(s) 3417
BspMI ACCTGC 4 cut(s) 582, 615, 3238, 3406
BspPI GGATC 8 cut(s) 920, 929, 2008, 2778, 3030, 3043, 3558, 3571
BspTNI GGTCTC 1 cut(s) 3305
BsrDI GCAATG 3 cut(s) 677, 2111, 2947
BsrI ACTGG 9 cut(s) 871, 1260, 1319, 1378, 1780, 2814, 2901, 2952, 3039
BssECI CCNNGG 9 cut(s) 199, 1017, 1139, 1528, 1549, 1634, 2251, 2593, 2909
BssNI GRCGYC 1 cut(s) 3521
BssSI CACGAG 2 cut(s) 888, 1565
BssT1I CCWWGG 5 cut(s) 199, 1549, 2251, 2593, 2909
Bst2BI CACGAG 2 cut(s) 888, 1565
Bst2UI CCWGG 8 cut(s) 212, 734, 1140, 1530, 1636, 1998, 2412, 3289
Bst6I CTCTTC 2 cut(s) 182, 804
BstACI GRCGYC 1 cut(s) 3521
BstBAI YACGTR 1 cut(s) 1955
BstC8I GCNNGC 5 cut(s) 1292, 2055, 2148, 2403, 3245
BstDSI CCRYGG 2 cut(s) 199, 1017
BstENI CCTNNNNNAGG 1 cut(s) 1137
BstFNI CGCG 2 cut(s) 1019, 3003
BstHHI GCGC 1 cut(s) 2104
BstMAI GTCTC 7 cut(s) 45, 1046, 1618, 1663, 2667, 2799, 3305
BstMCI CGRYCG 1 cut(s) 578
BstMWI GCNNNNNNNGC 7 cut(s) 491, 1163, 1757, 2059, 2152, 2494, 2946
BstNI CCWGG 8 cut(s) 212, 734, 1140, 1530, 1636, 1998, 2412, 3289
BstNSI RCATGY 1 cut(s) 2405
BstSCI CCNGG 9 cut(s) 210, 610, 732, 1138, 1528, 1634, 1996, 2410, 3287
BstSFI CTRYAG 2 cut(s) 1299, 3413
BstSLI GKGCMC 2 cut(s) 3268, 3688
BstUI CGCG 2 cut(s) 1019, 3003
BstV1I GCAGC 6 cut(s) 469, 741, 843, 893, 2699, 3682
BstV2I GAAGAC 5 cut(s) 109, 1086, 2769, 3279, 3306
BstX2I RGATCY 3 cut(s) 1477, 3035, 3563
BstYI RGATCY 3 cut(s) 1477, 3035, 3563
Bsu15I ATCGAT 1 cut(s) 2304
BsuTUI ATCGAT 1 cut(s) 2304
BtgI CCRYGG 2 cut(s) 199, 1017
BtrI CACGTC 1 cut(s) 1587
BtsI GCAGTG 3 cut(s) 1910, 2896, 3412
BveI ACCTGC 4 cut(s) 582, 615, 3238, 3406
Cac8I GCNNGC 5 cut(s) 1292, 2055, 2148, 2403, 3245
CaiI CAGNNNCTG 2 cut(s) 329, 1763
CfoI GCGC 1 cut(s) 2104
Cfr13I GGNCC 8 cut(s) 131, 776, 1235, 1334, 2495, 2693, 2729, 2779
Cfr42I CCGCGG 1 cut(s) 1020
ClaI ATCGAT 1 cut(s) 2304
CseI GACGC 1 cut(s) 350
CsiI ACCWGGT 1 cut(s) 3287
Csp6I GTAC 3 cut(s) 1045, 1307, 3042
CviQI GTAC 3 cut(s) 1045, 1307, 3042
DraI TTTAAA 1 cut(s) 2629
EaeI YGGCCR 2 cut(s) 868, 1561
Eam1104I CTCTTC 2 cut(s) 182, 804
EarI CTCTTC 2 cut(s) 182, 804
EciI GGCGGA 1 cut(s) 1846
Eco130I CCWWGG 5 cut(s) 199, 1549, 2251, 2593, 2909
Eco147I AGGCCT 1 cut(s) 1930
Eco31I GGTCTC 1 cut(s) 3305
Eco47I GGWCC 4 cut(s) 776, 1334, 2693, 2779
Eco57I CTGAAG 3 cut(s) 1137, 1921, 3318
Eco72I CACGTG 1 cut(s) 1955
Eco88I CYCGRG 1 cut(s) 2796
EcoNI CCTNNNNNAGG 1 cut(s) 1137
EcoO109I RGGNCCY 2 cut(s) 2693, 2779
EcoRI GAATTC 2 cut(s) 1990, 3358
EcoRII CCWGG 8 cut(s) 210, 732, 1138, 1528, 1634, 1996, 2410, 3287
EcoT14I CCWWGG 5 cut(s) 199, 1549, 2251, 2593, 2909
EcoT22I ATGCAT 1 cut(s) 253
ErhI CCWWGG 5 cut(s) 199, 1549, 2251, 2593, 2909
Esp3I CGTCTC 1 cut(s) 1663
FauI CCCGC 3 cut(s) 1024, 2225, 3004
FbaI TGATCA 2 cut(s) 1444, 1789
Fnu4HI GCNGC 8 cut(s) 483, 730, 857, 882, 1390, 1829, 2713, 3671
Fsp4HI GCNGC 8 cut(s) 483, 730, 857, 882, 1390, 1829, 2713, 3671
FspI TGCGCA 1 cut(s) 2103
GlaI GCGC 1 cut(s) 2103
GluI GCNGC 8 cut(s) 483, 730, 857, 882, 1390, 1829, 2713, 3671
GsuI CTGGAG 4 cut(s) 1797, 2918, 2935, 3140
HapII CCGG 2 cut(s) 612, 3269
HgaI GACGC 1 cut(s) 350
HhaI GCGC 1 cut(s) 2104
Hin1I GRCGYC 1 cut(s) 3521
Hin6I GCGC 1 cut(s) 2102
HinP1I GCGC 1 cut(s) 2102
HincII GTYRAC 2 cut(s) 295, 1285
HindII GTYRAC 2 cut(s) 295, 1285
HpaII CCGG 2 cut(s) 612, 3269
HpyCH4IV ACGT 4 cut(s) 1586, 1954, 3436, 3521
HpyF10VI GCNNNNNNNGC 7 cut(s) 491, 1163, 1757, 2059, 2152, 2494, 2946
HpySE526I ACGT 4 cut(s) 1586, 1954, 3436, 3521
Hsp92I GRCGYC 1 cut(s) 3521
HspAI GCGC 1 cut(s) 2102
Ksp22I TGATCA 2 cut(s) 1444, 1789
KspI CCGCGG 1 cut(s) 1020
LmnI GCTCC 5 cut(s) 16, 853, 1198, 2485, 2954
Lsp1109I GCAGC 6 cut(s) 469, 741, 843, 893, 2699, 3682
MabI ACCWGGT 1 cut(s) 3287
MaeII ACGT 4 cut(s) 1586, 1954, 3436, 3521
MaeIII GTNAC 7 cut(s) 338, 764, 1351, 1380, 2597, 2879, 3395
MfeI CAATTG 1 cut(s) 3612
MflI RGATCY 3 cut(s) 1477, 3035, 3563
MhlI GDGCHC 4 cut(s) 21, 466, 3268, 3688
MlsI TGGCCA 2 cut(s) 870, 1563
MluNI TGGCCA 2 cut(s) 870, 1563
MmeI TCCRAC 7 cut(s) 949, 1710, 2224, 2566, 2645, 3096, 3558
Mox20I TGGCCA 2 cut(s) 870, 1563
Mph1103I ATGCAT 1 cut(s) 253
MroXI GAANNNNTTC 1 cut(s) 1737
MscI TGGCCA 2 cut(s) 870, 1563
MslI CAYNNNNRTG 5 cut(s) 771, 876, 1610, 2292, 2539
Msp20I TGGCCA 2 cut(s) 870, 1563
MspA1I CMGCKG 3 cut(s) 494, 1019, 1715
MspI CCGG 2 cut(s) 612, 3269
MspR9I CCNGG 9 cut(s) 212, 612, 734, 1140, 1530, 1636, 1998, 2412, 3289
MunI CAATTG 1 cut(s) 3612
Mva1269I GAATGC 5 cut(s) 798, 912, 1011, 1470, 2018
MvaI CCWGG 8 cut(s) 212, 734, 1140, 1530, 1636, 1998, 2412, 3289
MvnI CGCG 2 cut(s) 1019, 3003
MwoI GCNNNNNNNGC 7 cut(s) 491, 1163, 1757, 2059, 2152, 2494, 2946
NciI CCSGG 1 cut(s) 612
NcoI CCATGG 1 cut(s) 199
NlaIV GGNNCC 6 cut(s) 2694, 2781, 2950, 3037, 3423, 3565
NmuCI GTSAC 5 cut(s) 338, 764, 1380, 2879, 3395
NsbI TGCGCA 1 cut(s) 2103
NsiI ATGCAT 1 cut(s) 253
NspI RCATGY 1 cut(s) 2405
PaeI GCATGC 1 cut(s) 2405
PaeR7I CTCGAG 1 cut(s) 2796
PaqCI CACCTGC 2 cut(s) 615, 3406
PceI AGGCCT 1 cut(s) 1930
PcsI WCGNNNNNNNCGW 1 cut(s) 575
PctI GAATGC 5 cut(s) 798, 912, 1011, 1470, 2018
PdmI GAANNNNTTC 1 cut(s) 1737
PflMI CCANNNNNTGG 2 cut(s) 850, 866
PfoI TCCNGGA 1 cut(s) 2410
PkrI GCNGC 8 cut(s) 484, 731, 858, 883, 1391, 1830, 2714, 3672
PmaCI CACGTG 1 cut(s) 1955
PmlI CACGTG 1 cut(s) 1955
Ppu21I YACGTR 1 cut(s) 1955
PpuMI RGGWCCY 2 cut(s) 2693, 2779
Psp1406I AACGTT 1 cut(s) 3436
Psp5II RGGWCCY 2 cut(s) 2693, 2779
Psp6I CCWGG 8 cut(s) 210, 732, 1138, 1528, 1634, 1996, 2410, 3287
PspCI CACGTG 1 cut(s) 1955
PspGI CCWGG 8 cut(s) 210, 732, 1138, 1528, 1634, 1996, 2410, 3287
PspN4I GGNNCC 6 cut(s) 2694, 2781, 2950, 3037, 3423, 3565
PspPI GGNCC 8 cut(s) 131, 776, 1235, 1334, 2495, 2693, 2729, 2779
PspPPI RGGWCCY 2 cut(s) 2693, 2779
PstI CTGCAG 1 cut(s) 3417
PstNI CAGNNNCTG 2 cut(s) 329, 1763
PsuI RGATCY 3 cut(s) 1477, 3035, 3563
PvuII CAGCTG 1 cut(s) 1715
RsaI GTAC 3 cut(s) 1046, 1308, 3043
RsaNI GTAC 3 cut(s) 1045, 1307, 3042
RseI CAYNNNNRTG 5 cut(s) 771, 876, 1610, 2292, 2539
SacII CCGCGG 1 cut(s) 1020
SatI GCNGC 8 cut(s) 483, 730, 857, 882, 1390, 1829, 2713, 3671
Sau96I GGNCC 8 cut(s) 131, 776, 1235, 1334, 2495, 2693, 2729, 2779
ScaI AGTACT 1 cut(s) 3043
ScrFI CCNGG 9 cut(s) 212, 612, 734, 1140, 1530, 1636, 1998, 2412, 3289
SduI GDGCHC 4 cut(s) 21, 466, 3268, 3688
SexAI ACCWGGT 1 cut(s) 3287
SfcI CTRYAG 2 cut(s) 1299, 3413
Sfr274I CTCGAG 1 cut(s) 2796
Sfr303I CCGCGG 1 cut(s) 1020
SgrBI CCGCGG 1 cut(s) 1020
SinI GGWCC 4 cut(s) 776, 1334, 2693, 2779
SlaI CTCGAG 1 cut(s) 2796
SmiMI CAYNNNNRTG 5 cut(s) 771, 876, 1610, 2292, 2539
SmlI CTYRAG 1 cut(s) 2796
SmoI CTYRAG 1 cut(s) 2796
SpeI ACTAGT 1 cut(s) 964
SphI GCATGC 1 cut(s) 2405
SseBI AGGCCT 1 cut(s) 1930
SspI AATATT 3 cut(s) 238, 1126, 2616
StuI AGGCCT 1 cut(s) 1930
StyD4I CCNGG 9 cut(s) 210, 610, 732, 1138, 1528, 1634, 1996, 2410, 3287
StyI CCWWGG 5 cut(s) 199, 1549, 2251, 2593, 2909
TaiI ACGT 4 cut(s) 1589, 1957, 3439, 3524
TaqI TCGA 6 cut(s) 505, 578, 961, 2304, 2797, 3390
TaqII GACCGA 2 cut(s) 564, 3329
TatI WGTACW 2 cut(s) 1306, 3041
TauI GCSGC 2 cut(s) 1392, 1831
TseFI GTSAC 5 cut(s) 338, 764, 1380, 2879, 3395
TseI GCWGC 6 cut(s) 482, 729, 856, 881, 2712, 3670
Tsp45I GTSAC 5 cut(s) 338, 764, 1380, 2879, 3395
TspGWI ACGGA 1 cut(s) 3048
Van91I CCANNNNNTGG 2 cut(s) 850, 866
VneI GTGCAC 1 cut(s) 3264
VpaK11BI GGWCC 4 cut(s) 776, 1334, 2693, 2779
XagI CCTNNNNNAGG 1 cut(s) 1137
XceI RCATGY 1 cut(s) 2405
XhoI CTCGAG 1 cut(s) 2796
XmnI GAANNNNTTC 1 cut(s) 1737
ZraI GACGTC 1 cut(s) 3522
ZrmI AGTACT 1 cut(s) 3043
Zsp2I ATGCAT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.