Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007187

Physical Location & Seq

Genomic Coordinates Forward (+)

38726981 .. 38731454

Gene Structure 4474 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1256100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3057 bp

ATGGAACGAGGCGGTGGCGGTGGATTTAGGGGAAGGGGAAGAGGAAGAGGGAGAGGGAATGATGGTGGTGGTGGTGGAAGAGGTGATCCTGGCGGCTATGGCAGAGGAACTGGAGGGAGAAGTGGAGGAAGAGGAGGAGGAGGAGAGAGAGGAAGAGGCGGTCATGGCGGCGGTGTGAATGTTGGCGGGCCACAGTTCCACCACCATCATCCGCAGCCACAGCCGCCACTGCAACAGCAGCCACAGCAACAGCAACCGCCGAATCAGAATCCGAGTCAACAACCGAGGCCTAATCCATGGAATACTCCCGCTTCTACCAGAGCATGGGGGCCCAGAGCGCCTGCTCCGCCTGCATCTCCGGTCGGATCGGCCCCGAACATCTCTCCTGATCCTGGTACTCTTGTTCCTGCAATGCAATCGATGGCAATTTCAAGTCAGACACCCTCTTCATCTTCTTTGGAAAAGGCGAGCAATCATGTGCCAGTGAAGCGGCCTGATCGTGGGGGGATGATAGCTATCCGAACTGCTAAGCTCCGTGCTAATCATTTTAATCTCAAGTTCAATCCTGAGAGTATTATAATGCACTATGATGTTGATGTTAAGCCAGAACACCCTGCTAAGAATGGCCGTCCGGTGAAGATATCAAAGTCTGAACTTGCTGCAATCAGGAAGCAGTTATCCACGGATGATCCTTCGAATTTTCCATTGTTAAGTACAGCATATGATGGTGAAAAGAACATATTCAGTGCAGTGCCATTGCCCACTGGATCATTTAAGGTGGAAGTTCCTGAGGAAGAAGGCACGCGGTTCAGTTCGTACATTTTTACTATAAAGCTTGCAAAGGAGCTGCAGCTTTGCAAGTTGAAGGAGTACCTAAGTGGTCAGCTGTTGGATATCCCTCGTAACATATTACAGAGTATGGACTTGGTGATGAAAGAGAATCCAACTAGGCGCATGATTGCTTTTGGGCGAAACTTTTACCCCACTGAATCTCATCCAAACGATGACCTTGGTTTTGGTGTTGCTGCCTTCAGAGGCTTTCAGCATGGCTTGAAGCTCACTTCTCAGGGTCCTACCTTGTGTCTGGATTACTCAGTCTTGGCATTTCATAAGCGCATGTCAGTTATTGAGTTTCTCCAACAGCAAATAAGGGGATTTACTTTAAATAATTTCCAGAGTTATAGGAGAGATGTTGAGGATGTATTGAAGGGATTGCAAGTTAACGTGACTCACCGCAGAACCAAGCAGAAGTACCTCATTAAGGGGCTTACTGATAGGAATGCAGGAGATATTACATTTGATGCTGTAGACATAGATGGCCAGAGTCCACCTACGAAAGTTAGACTTCTTGATTATTTCAGGGACAAATATGAGGAGATTCGGTACAAAAACATTCCTTGCTTGGATTTGGGGAAAAATGGTAGAAGGAATGATACCCCAATGGAGTTCTGTGTCCTAGTTGAGGGGCAAAGGTATCCAAAGGAGTATTTGGGCAAACAAGCAGCCATCATGCTGAAGAACATCTCATTGCCTTCACCAAGAGATAGACAGAATAGTATATACAACATGGTACGATCAAATGATGGACCTTGCGGTGGTGGCATCATTCAGAATTTTGGATTTGAAGTCAACCAGAATATGACACCAGTAACAGGGCGTGTGATAGGCCCTCCTGAGTTGAAGTTGGGTGCTTCTGATGGCAAGGTGGTGAATAAGGTGACTGTTGACAGAGAGAAATGCCAATGGAATCTGGTTGGGAAGAGAGTTGTGGAAGGAAAACCAATCATGCGTTGGGCTGTACTTGACTTCAGCAGTAATGATCGTTATAATACACTGGACCCTAACCAGTTCATCCCAAAGCTCATCGCTCGGTGCAACAAACTGGGAATCAGAATGGAAGAGCCTGTTTTGTATGAAACCACTTCCATGAGGCCATTCTCTAGTGTTCAGATGCTTCGTCAGCTGCTTGAAAACGTTAATGAAAAGGCCAAAAAAGAAGGCAAAGGTTACTTGCAACTACTTGTTTGTGTAATGGCAAACAGAGACCCTGGTTACAAGTATCTGAAGTGGATCGCTGAGACTCAAATTGGTATAGTGACACAGTGCTGCTTGTCAAATATGGCCAACAAAGCAAATGACCAGTACCTTGCAAATCTTGCACTCAAGATCAATGCCAAGCTTGGAGGCAGTAATGTAGAGCTAATTGATCGGCTCCCCCTCTTAGGGGGTGCAGGCCATGTTATGTTTGTGGGGGCTGATGTCAATCATCCTGCTGCGCGAAACACAACAAGTCCATCAATTGCAGCTGTTGTTGCCACTGTAAACTGGCCTGCTGCGAATCGTTATGCTGCACGAGTTCGACCCCAGTACCATCGTAAGGAGAAGATAATAAATTTTGGAGACATGTGTCTCGAGCTTGTTCAAACTTACGAGCGGTTGAATAAAGTCAAGCCAAACCAAATTGTTGTCTTCCGTGATGGAGTCAGTGAGGGCCAGTTTGATATGGTTCTCAATGAAGAGTTGCTAGATATGAAGATGGCTTTGGCAAGTATAAAATACTATCCAACCATCACACTTATTGTTGCCCAGAAGCGGCACCATACTCGTCTGTTTTTGGAGAATCCAAGGGATGGGGGTTCCACAGGCAATATATCTCCGGGAACTGTTGTGGACACCACAATCGTGCACCCGTTTGAGTTTGACTTCTATCTCTGCAGTCATTATGGAGCCCTTGGGACAAGCAAACCCACGCACTACCATGTTCTTTGGGACGAGCACCGGTTTACTTCTGACCAATTGCAGAAGCTCATATATGACTTGTGCTTCACTTTTGCAAGGTGTACAAAACCTGTATCATTGGTTCCACCGGTGTACTATGCTGACCTTGTGGCATATAGGGGGCGATTATACTTTGAGAGTACGGAGGGACAGTCTCCAGCGTCAGTATCCTCCGCCTCCTCCTCATCCTCGACACATTCACCGCTGTCAGTGTCTTCTCTCGATGAAAGGTTTTGCAAGTTGCACGCGGAACTGGAAAACATAATGTTTTTCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0016032 GO:0019048 GO:0035197 GO:0035821 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0061980 GO:0097159 GO:0098542 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

1019

Amino Acids

113.0

Weight (kDa)

9.4

Isoelectric Point (pI)

44.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	175 - 311	4.4e-23	N-terminal domain of argonaute
ArgoL1	PF08699	322 - 371	1.9e-08	Argonaute linker 1 domain
PAZ	PF02170	390 - 497	2e-20	PAZ domain
ArgoL2	PF16488	515 - 561	3.8e-07	Argonaute linker 2 domain
ArgoMid	PF16487	581 - 634	9.6e-07	Mid domain of argonaute
Piwi	PF02171	674 - 968	4.1e-93	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31280 AT1G31290 AT1G31290 AT1G31290
fragaria_vesca	FvH4_3g11980
malus_domestica	MD05G1256100.v1.1 MD10G1236300.v1.1 MD10G1236400.v1.1
prunus_persica	Prupe.4G108000_v2.0.a1 Prupe.4G108100_v2.0.a1
pyrus_communis	pycom05g23500 pycom05g23510 pycom05g23520 pycom10g19770 pycom10g19780
rosa_chinensis	RchiOBHm_Chr5g0019581
rosa_laevigata	RLG00000032457
rosa_roxburghii	Rroxscaffold_1G00058500
rosa_rugosa	Rorug05G0052100 Rorug05G0052200
rosa_samantha	Rh5AG142700 Rh5BG141200 Rh5CG153300 Rh5DG141900
rosa_wichuraiana	Rw5G012630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	578, 1827
AccB1I	GGYRCC	1 cut(s)	2595
AccB7I	CCANNNNNTGG	2 cut(s)	324, 2630
AccBSI	CCGCTC	1 cut(s)	2434
AccI	GTMKAC	1 cut(s)	1308
AccII	CGCG	3 cut(s)	805, 2278, 3026
AclI	AACGTT	1 cut(s)	1974
AclWI	GGATC	6 cut(s)	80, 373, 383, 683, 775, 2078
AcoI	YGGCCR	3 cut(s)	625, 1318, 2121
AcsI	RAATTY	3 cut(s)	697, 1612, 2392
AcuI	CTGAAG	4 cut(s)	1015, 1535, 1792, 2084
AflIII	ACRYGT	1 cut(s)	2403
AgeI	ACCGGT	2 cut(s)	2778, 2866
AjnI	CCWGG	3 cut(s)	88, 391, 2047
AjuI	GAANNNNNNNTTGG	2 cut(s)	2525, 2557
AleI	CACNNNNGTG	1 cut(s)	2681
Alw21I	GWGCWC	2 cut(s)	2688, 2778
Alw26I	GTCTC	5 cut(s)	2037, 2072, 2394, 2414, 2937
Alw44I	GTGCAC	1 cut(s)	2684
AlwI	GGATC	6 cut(s)	80, 373, 383, 683, 775, 2078
Ama87I	CYCGRG	1 cut(s)	2411
ApaI	GGGCCC	1 cut(s)	333
ApaLI	GTGCAC	1 cut(s)	2684
ApoI	RAATTY	3 cut(s)	697, 1612, 2392
ArsI	GACNNNNNNTTYG	2 cut(s)	998, 1030
AsiGI	ACCGGT	2 cut(s)	2778, 2866
Asp700I	GAANNNNTTC	1 cut(s)	740
AspLEI	GCGC	4 cut(s)	340, 954, 1116, 2278
AspS9I	GGNCC	9 cut(s)	188, 329, 330, 370, 1070, 1586, 1667, 1837, 2491
AsuC2I	CCSGG	1 cut(s)	2658
AsuHPI	GGTGA	9 cut(s)	95, 646, 740, 940, 1223, 1527, 1720, 1729, 2970
AsuII	TTCGAA	1 cut(s)	695
AvaI	CYCGRG	1 cut(s)	2411
AvaII	GGWCC	3 cut(s)	1070, 1586, 1837
AxyI	CCTNAGG	1 cut(s)	789
BaeGI	GKGCMC	2 cut(s)	333, 2688
BaeI	ACNNNNGTAYC	4 cut(s)	387, 420, 2351, 2384
BalI	TGGCCA	2 cut(s)	1320, 2123
BanI	GGYRCC	1 cut(s)	2595
BanII	GRGCYC	2 cut(s)	333, 2731
BarI	GAAGNNNNNNTAC	2 cut(s)	1417, 1449
BauI	CACGAG	1 cut(s)	2352
BbsI	GAAGAC	2 cut(s)	2461, 2985
Bbv12I	GWGCWC	2 cut(s)	2688, 2778
BceAI	ACGGC	1 cut(s)	612
BcgI	CGANNNNNNTGC	4 cut(s)	399, 433, 2585, 2619
BciT130I	CCWGG	3 cut(s)	90, 393, 2049
BciVI	GTATCC	2 cut(s)	1485, 2956
BcnI	CCSGG	1 cut(s)	2658
BcoDI	GTCTC	5 cut(s)	2037, 2072, 2394, 2414, 2937
BfaI	CTAG	4 cut(s)	948, 1457, 1941, 2525
BfmI	CTRYAG	3 cut(s)	848, 1305, 2713
BfoI	RGCGCY	1 cut(s)	341
BfuI	GTATCC	2 cut(s)	1485, 2956
BlpI	GCTNAGC	1 cut(s)	528
Bme1390I	CCNGG	4 cut(s)	90, 393, 2049, 2658
Bme18I	GGWCC	3 cut(s)	1070, 1586, 1837
BmeT110I	CYCGRG	1 cut(s)	2411
BmgT120I	GGNCC	9 cut(s)	188, 329, 330, 370, 1070, 1586, 1667, 1837, 2491
BmrFI	CCNGG	4 cut(s)	90, 393, 2049, 2658
BmrI	ACTGGG	2 cut(s)	1892, 2359
BmsI	GCATC	4 cut(s)	362, 1291, 1611, 1941
BmuI	ACTGGG	2 cut(s)	1892, 2359
BoxI	GACNNNNGTC	1 cut(s)	2406
BpiI	GAAGAC	2 cut(s)	2461, 2985
BpmI	CTGGAG	2 cut(s)	132, 2919
Bpu1102I	GCTNAGC	1 cut(s)	528
Bpu14I	TTCGAA	1 cut(s)	695
BpuEI	CTTGAG	2 cut(s)	539, 2147
BpuMI	CCSGG	1 cut(s)	2658
Bsa29I	ATCGAT	1 cut(s)	419
BsaBI	GATNNNNATC	2 cut(s)	515, 2262
BsaI	GGTCTC	1 cut(s)	2037
BsaJI	CCNNGG	7 cut(s)	284, 296, 681, 1009, 2047, 2624, 2731
BsaWI	WCCGGW	4 cut(s)	358, 631, 2778, 2866
BsaXI	ACNNNNNCTCC	4 cut(s)	1367, 1397, 2915, 2945
Bse118I	RCCGGY	2 cut(s)	2778, 2866
Bse21I	CCTNAGG	1 cut(s)	789
Bse3DI	GCAATG	3 cut(s)	417, 755, 1526
Bse8I	GATNNNNATC	2 cut(s)	515, 2262
BseBI	CCWGG	3 cut(s)	90, 393, 2049
BseCI	ATCGAT	1 cut(s)	419
BseDI	CCNNGG	7 cut(s)	284, 296, 681, 1009, 2047, 2624, 2731
BseGI	GGATG	9 cut(s)	208, 513, 691, 994, 1204, 1851, 2266, 2635, 2963
BseJI	GATNNNNATC	2 cut(s)	515, 2262
BseMI	GCAATG	3 cut(s)	417, 755, 1526
BseMII	CTCAG	6 cut(s)	558, 780, 1079, 1107, 1665, 2067
BseRI	GAGGAG	7 cut(s)	147, 150, 153, 156, 1388, 2947, 2950
BseSI	GKGCMC	2 cut(s)	333, 2688
BsgI	GTGCAG	3 cut(s)	768, 2250, 2334
Bsh1236I	CGCG	3 cut(s)	805, 2278, 3026
Bsh1285I	CGRYCG	1 cut(s)	363
BshNI	GGYRCC	1 cut(s)	2595
BshTI	ACCGGT	2 cut(s)	2778, 2866
BshVI	ATCGAT	1 cut(s)	419
BsiEI	CGRYCG	1 cut(s)	363
BsiHKAI	GWGCWC	2 cut(s)	2688, 2778
BsiHKCI	CYCGRG	1 cut(s)	2411
BsiSI	CCGG	5 cut(s)	359, 632, 2657, 2779, 2867
BslFI	GGGAC	4 cut(s)	1376, 2749, 2783, 2940
BsmAI	GTCTC	5 cut(s)	2037, 2072, 2394, 2414, 2937
BsmFI	GGGAC	4 cut(s)	1376, 2749, 2783, 2940
BsmI	GAATGC	1 cut(s)	1285
Bso31I	GGTCTC	1 cut(s)	2037
BsoBI	CYCGRG	1 cut(s)	2411
Bsp119I	TTCGAA	1 cut(s)	695
Bsp120I	GGGCCC	1 cut(s)	329
Bsp1286I	GDGCHC	4 cut(s)	333, 2688, 2731, 2778
Bsp1407I	TGTACA	1 cut(s)	2840
Bsp1720I	GCTNAGC	1 cut(s)	528
Bsp19I	CCATGG	1 cut(s)	296
BspCNI	CTCAG	6 cut(s)	559, 781, 1078, 1106, 1666, 2068
BspDI	ATCGAT	1 cut(s)	419
BspFNI	CGCG	3 cut(s)	805, 2278, 3026
BspMAI	CTGCAG	2 cut(s)	852, 2717
BspPI	GGATC	6 cut(s)	80, 373, 383, 683, 775, 2078
BspQI	GCTCTTC	1 cut(s)	1893
BspT104I	TTCGAA	1 cut(s)	695
BspT107I	GGYRCC	1 cut(s)	2595
BspTNI	GGTCTC	1 cut(s)	2037
BsrBI	CCGCTC	1 cut(s)	2434
BsrDI	GCAATG	3 cut(s)	417, 755, 1526
BsrFI	RCCGGY	2 cut(s)	2778, 2866
BsrGI	TGTACA	1 cut(s)	2840
BssAI	RCCGGY	2 cut(s)	2778, 2866
BssECI	CCNNGG	7 cut(s)	284, 296, 681, 1009, 2047, 2624, 2731
BssSI	CACGAG	1 cut(s)	2352
BssT1I	CCWWGG	4 cut(s)	296, 1009, 2624, 2731
Bst2BI	CACGAG	1 cut(s)	2352
Bst2UI	CCWGG	3 cut(s)	90, 393, 2049
Bst4CI	ACNGT	6 cut(s)	195, 1723, 2103, 2320, 2665, 2931
Bst6I	CTCTTC	9 cut(s)	34, 40, 73, 124, 148, 451, 1754, 1893, 2511
BstAPI	GCANNNNNTGC	1 cut(s)	2156
BstAUI	TGTACA	1 cut(s)	2840
BstBI	TTCGAA	1 cut(s)	695
BstC8I	GCNNGC	9 cut(s)	188, 342, 351, 469, 803, 837, 2233, 2331, 3024
BstDSI	CCRYGG	2 cut(s)	296, 681
BstENI	CCTNNNNNAGG	2 cut(s)	1259, 1460
BstF5I	GGATG	9 cut(s)	208, 513, 691, 994, 1204, 1851, 2266, 2635, 2963
BstFNI	CGCG	3 cut(s)	805, 2278, 3026
BstH2I	RGCGCY	1 cut(s)	341
BstHHI	GCGC	4 cut(s)	340, 954, 1116, 2278
BstMAI	GTCTC	5 cut(s)	2037, 2072, 2394, 2414, 2937
BstMCI	CGRYCG	1 cut(s)	363
BstNI	CCWGG	3 cut(s)	90, 393, 2049
BstNSI	RCATGY	2 cut(s)	1120, 2407
BstPAI	GACNNNNGTC	1 cut(s)	2406
BstSCI	CCNGG	4 cut(s)	88, 391, 2047, 2656
BstSFI	CTRYAG	3 cut(s)	848, 1305, 2713
BstSLI	GKGCMC	2 cut(s)	333, 2688
BstUI	CGCG	3 cut(s)	805, 2278, 3026
BstV2I	GAAGAC	2 cut(s)	2461, 2985
Bsu15I	ATCGAT	1 cut(s)	419
Bsu36I	CCTNAGG	1 cut(s)	789
BsuI	GTATCC	2 cut(s)	1485, 2956
BsuTUI	ATCGAT	1 cut(s)	419
BtgI	CCRYGG	2 cut(s)	296, 681
BtgZI	GCGATG	1 cut(s)	1849
BtsCI	GGATG	9 cut(s)	208, 513, 691, 994, 1204, 1851, 2266, 2635, 2963
BtsI	GCAGTG	2 cut(s)	227, 756
Cac8I	GCNNGC	9 cut(s)	188, 342, 351, 469, 803, 837, 2233, 2331, 3024
CfoI	GCGC	4 cut(s)	340, 954, 1116, 2278
Cfr10I	RCCGGY	2 cut(s)	2778, 2866
Cfr13I	GGNCC	9 cut(s)	188, 329, 330, 370, 1070, 1586, 1667, 1837, 2491
ClaI	ATCGAT	1 cut(s)	419
CseI	GACGC	1 cut(s)	2928
CspAI	ACCGGT	2 cut(s)	2778, 2866
DraI	TTTAAA	1 cut(s)	1164
EaeI	YGGCCR	3 cut(s)	625, 1318, 2121
Eam1104I	CTCTTC	9 cut(s)	34, 40, 73, 124, 148, 451, 1754, 1893, 2511
EarI	CTCTTC	9 cut(s)	34, 40, 73, 124, 148, 451, 1754, 1893, 2511
EciI	GGCGGA	2 cut(s)	336, 2941
Eco130I	CCWWGG	4 cut(s)	296, 1009, 2624, 2731
Eco147I	AGGCCT	1 cut(s)	289
Eco24I	GRGCYC	2 cut(s)	333, 2731
Eco31I	GGTCTC	1 cut(s)	2037
Eco32I	GATATC	2 cut(s)	642, 895
Eco47I	GGWCC	3 cut(s)	1070, 1586, 1837
Eco57I	CTGAAG	4 cut(s)	1015, 1535, 1792, 2084
Eco81I	CCTNAGG	1 cut(s)	789
Eco88I	CYCGRG	1 cut(s)	2411
EcoNI	CCTNNNNNAGG	2 cut(s)	1259, 1460
EcoO109I	RGGNCCY	3 cut(s)	329, 1070, 1667
EcoRII	CCWGG	3 cut(s)	88, 391, 2047
EcoRV	GATATC	2 cut(s)	642, 895
EcoT14I	CCWWGG	4 cut(s)	296, 1009, 2624, 2731
EcoT38I	GRGCYC	2 cut(s)	333, 2731
ErhI	CCWWGG	4 cut(s)	296, 1009, 2624, 2731
FalI	AAGNNNNNCTT	2 cut(s)	1329, 1361
FaqI	GGGAC	4 cut(s)	1376, 2749, 2783, 2940
FauI	CCCGC	2 cut(s)	179, 316
FauNDI	CATATG	1 cut(s)	721
FblI	GTMKAC	1 cut(s)	1308
FokI	GGATG	9 cut(s)	195, 520, 698, 981, 1211, 1838, 2253, 2642, 2950
FriOI	GRGCYC	2 cut(s)	333, 2731
FspBI	CTAG	4 cut(s)	948, 1457, 1941, 2525
GlaI	GCGC	4 cut(s)	339, 953, 1115, 2277
GsuI	CTGGAG	2 cut(s)	132, 2919
HaeII	RGCGCY	1 cut(s)	341
HapII	CCGG	5 cut(s)	359, 632, 2657, 2779, 2867
HgaI	GACGC	1 cut(s)	2928
HhaI	GCGC	4 cut(s)	340, 954, 1116, 2278
Hin6I	GCGC	4 cut(s)	338, 952, 1114, 2276
HinP1I	GCGC	4 cut(s)	338, 952, 1114, 2276
HincII	GTYRAC	4 cut(s)	278, 1222, 1630, 1726
HindII	GTYRAC	4 cut(s)	278, 1222, 1630, 1726
HindIII	AAGCTT	2 cut(s)	833, 2177
HpaI	GTTAAC	1 cut(s)	1222
HpaII	CCGG	5 cut(s)	359, 632, 2657, 2779, 2867
HphI	GGTGA	9 cut(s)	95, 646, 740, 940, 1223, 1527, 1720, 1729, 2970
HpyCH4III	ACNGT	6 cut(s)	195, 1723, 2103, 2320, 2665, 2931
HpyCH4IV	ACGT	2 cut(s)	1224, 1974
HpySE526I	ACGT	2 cut(s)	1224, 1974
HspAI	GCGC	4 cut(s)	338, 952, 1114, 2276
KspAI	GTTAAC	1 cut(s)	1222
LguI	GCTCTTC	1 cut(s)	1893
LmnI	GCTCC	5 cut(s)	349, 537, 844, 2217, 2726
LweI	GCATC	4 cut(s)	362, 1291, 1611, 1941
MaeI	CTAG	4 cut(s)	948, 1457, 1941, 2525
MaeII	ACGT	2 cut(s)	1224, 1974
MaeIII	GTNAC	7 cut(s)	902, 1225, 1648, 1717, 2006, 2051, 2095
MbiI	CCGCTC	1 cut(s)	2434
MfeI	CAATTG	2 cut(s)	2298, 2795
MhlI	GDGCHC	4 cut(s)	333, 2688, 2731, 2778
MlsI	TGGCCA	2 cut(s)	1320, 2123
MluNI	TGGCCA	2 cut(s)	1320, 2123
MlyI	GAGTC	5 cut(s)	283, 1222, 1333, 2074, 2490
MmeI	TCCRAC	5 cut(s)	343, 870, 968, 1162, 2588
Mox20I	TGGCCA	2 cut(s)	1320, 2123
MroXI	GAANNNNTTC	1 cut(s)	740
MscI	TGGCCA	2 cut(s)	1320, 2123
MseI	TTAA	8 cut(s)	549, 600, 710, 774, 1163, 1221, 1260, 1977
MslI	CAYNNNNRTG	4 cut(s)	588, 1925, 2681, 2757
Msp20I	TGGCCA	2 cut(s)	1320, 2123
MspA1I	CMGCKG	4 cut(s)	886, 1963, 2306, 2983
MspI	CCGG	5 cut(s)	359, 632, 2657, 2779, 2867
MspR9I	CCNGG	4 cut(s)	90, 393, 2049, 2658
MunI	CAATTG	2 cut(s)	2298, 2795
Mva1269I	GAATGC	1 cut(s)	1285
MvaI	CCWGG	3 cut(s)	90, 393, 2049
MvnI	CGCG	3 cut(s)	805, 2278, 3026
NciI	CCSGG	1 cut(s)	2658
NcoI	CCATGG	1 cut(s)	296
NdeI	CATATG	1 cut(s)	721
NmuCI	GTSAC	3 cut(s)	1225, 1717, 2095
NspI	RCATGY	2 cut(s)	1120, 2407
NspV	TTCGAA	1 cut(s)	695
OliI	CACNNNNGTG	1 cut(s)	2681
PaeR7I	CTCGAG	1 cut(s)	2411
PceI	AGGCCT	1 cut(s)	289
PciI	ACATGT	1 cut(s)	2403
PciSI	GCTCTTC	1 cut(s)	1893
PctI	GAATGC	1 cut(s)	1285
PdmI	GAANNNNTTC	1 cut(s)	740
PfeI	GAWTC	9 cut(s)	262, 268, 940, 989, 1378, 1747, 1887, 2338, 2620
PflMI	CCANNNNNTGG	2 cut(s)	324, 2630
PfoI	TCCNGGA	1 cut(s)	2656
PinAI	ACCGGT	2 cut(s)	2778, 2866
PleI	GAGTC	5 cut(s)	282, 1222, 1332, 2074, 2489
PpsI	GAGTC	5 cut(s)	282, 1222, 1332, 2074, 2489
PpuMI	RGGWCCY	1 cut(s)	1070
PscI	ACATGT	1 cut(s)	2403
PshAI	GACNNNNGTC	1 cut(s)	2406
PsiI	TTATAA	2 cut(s)	578, 1827
Psp1406I	AACGTT	1 cut(s)	1974
Psp5II	RGGWCCY	1 cut(s)	1070
Psp6I	CCWGG	3 cut(s)	88, 391, 2047
PspGI	CCWGG	3 cut(s)	88, 391, 2047
PspOMI	GGGCCC	1 cut(s)	329
PspPI	GGNCC	9 cut(s)	188, 329, 330, 370, 1070, 1586, 1667, 1837, 2491
PspPPI	RGGWCCY	1 cut(s)	1070
PsrI	GAACNNNNNNTAC	2 cut(s)	2844, 2876
PstI	CTGCAG	2 cut(s)	852, 2717
PvuII	CAGCTG	3 cut(s)	886, 1963, 2306
RseI	CAYNNNNRTG	4 cut(s)	588, 1925, 2681, 2757
SapI	GCTCTTC	1 cut(s)	1893
SaqAI	TTAA	8 cut(s)	549, 600, 710, 774, 1163, 1221, 1260, 1977
Sau96I	GGNCC	9 cut(s)	188, 329, 330, 370, 1070, 1586, 1667, 1837, 2491
SchI	GAGTC	5 cut(s)	283, 1222, 1333, 2074, 2490
ScrFI	CCNGG	4 cut(s)	90, 393, 2049, 2658
SduI	GDGCHC	4 cut(s)	333, 2688, 2731, 2778
SfaNI	GCATC	4 cut(s)	362, 1291, 1611, 1941
SfcI	CTRYAG	3 cut(s)	848, 1305, 2713
Sfr274I	CTCGAG	1 cut(s)	2411
SfuI	TTCGAA	1 cut(s)	695
SgrAI	CRCCGGYG	1 cut(s)	2866
SinI	GGWCC	3 cut(s)	1070, 1586, 1837
SlaI	CTCGAG	1 cut(s)	2411
SmiMI	CAYNNNNRTG	4 cut(s)	588, 1925, 2681, 2757
SmlI	CTYRAG	3 cut(s)	554, 2162, 2411
SmoI	CTYRAG	3 cut(s)	554, 2162, 2411
SseBI	AGGCCT	1 cut(s)	289
SspMI	CTAG	4 cut(s)	948, 1457, 1941, 2525
StuI	AGGCCT	1 cut(s)	289
StyD4I	CCNGG	4 cut(s)	88, 391, 2047, 2656
StyI	CCWWGG	4 cut(s)	296, 1009, 2624, 2731
TaaI	ACNGT	6 cut(s)	195, 1723, 2103, 2320, 2665, 2931
TaiI	ACGT	2 cut(s)	1227, 1977
TaqI	TCGA	6 cut(s)	419, 695, 2359, 2412, 2969, 3000
TatI	WGTACW	4 cut(s)	713, 1798, 2840, 2871
TauI	GCSGC	5 cut(s)	96, 171, 226, 493, 2596
TfiI	GAWTC	9 cut(s)	262, 268, 940, 989, 1378, 1747, 1887, 2338, 2620
Tru1I	TTAA	8 cut(s)	549, 600, 710, 774, 1163, 1221, 1260, 1977
Tru9I	TTAA	8 cut(s)	549, 600, 710, 774, 1163, 1221, 1260, 1977
TseFI	GTSAC	3 cut(s)	1225, 1717, 2095
Tsp45I	GTSAC	3 cut(s)	1225, 1717, 2095
TspDTI	ATGAA	9 cut(s)	438, 947, 1097, 1840, 1929, 1995, 2529, 2546, 3018
TspGWI	ACGGA	4 cut(s)	524, 698, 2462, 2936
Van91I	CCANNNNNTGG	2 cut(s)	324, 2630
VneI	GTGCAC	1 cut(s)	2684
VpaK11BI	GGWCC	3 cut(s)	1070, 1586, 1837
XagI	CCTNNNNNAGG	2 cut(s)	1259, 1460
XapI	RAATTY	3 cut(s)	697, 1612, 2392
XceI	RCATGY	2 cut(s)	1120, 2407
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1788
XhoI	CTCGAG	1 cut(s)	2411
XmiI	GTMKAC	1 cut(s)	1308
XmnI	GAANNNNTTC	1 cut(s)	740
XspI	CTAG	4 cut(s)	948, 1457, 1941, 2525

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.