Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0007187

Physical Location & Seq

Genomic Coordinates Forward (+)

25386381 .. 25395861

Gene Structure 9481 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g23520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7044 bp

ATGACAAAAAACTACCTCAACTATTGGTCTGACAACACTTTCATACCTCATCTTCTAAAAGTGACAATGTCATACCGCATCTTACGAATTTGTGCCAATGTCATATCTCTGTCAGTTTTTCTGTTAATTTCTCTGTTAAATGCTGACTTGGCTAGAGACAGGGGATCTGACCTTCCCTTTCCTCCACCTGCACCAACCCTTTTTCTTCTTCTCTTCGACAGCCACCAAACCCTTCCCCGTCCCCCTAACCTTCCTTCATCACCCTCTTTCTCTCTTCTCTCTCTTCTCTCTCTGGGATGCGATCTACCTCCTCCATCCCTCTCCCCATTCGCACTCGTCCTTCCCTCACCACCACCTCCCGACCCGACCCATCCTTGTCTTAGTCCCTCCATACCCAACCTTCCTTCGCCACCCCACCGCCGCCTCTCTTCCTCCATCTTCACCACCCCACTGAAGAAACCAAAAAAACCAAGCCCACACCCAGATCCCACACCCAAAAAAAAAAAACCAAACCCACACCCAGATCCCCTTCCCCTCTTACACACCCAAACCCTCAATTGCCTCCCCACATTTCGCCGCGACCCGTTGTCGCCACCCTGGGCTTCTAACCTCGCCGCCTCCTTGTGCCAGCTTGTGCACAATTATGCCTGGGACCCCATGGGAAGGAGAAAGATGGCGAGTTGGCGGACGCTGGCCTCGGCAGATGCGAGAGAAGATAAAGAGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGAGTTGGTGTGGGTGGAAGAAAGGAAGGTCTGCATCCCTAGGCATTTTTTATTTGTTTAGTTTTTTAATTTTTGAATATTTTATTATTAAAAATATATTTTATTATTTTTTAATCCAATTGGATTATGACGGAGGTTTGACATTGGCACAAATTCATAAGATGAGATATGATAGTGTCTTCTCTACAATCTCACAATTCTCAAATACAACTTCAATTACTTTATTCTTCTCCATAAGGAGGTATTTACAGGATTACAAAAGAGCAATACTAGGAAATAAATCAATACAATCCTTTCATATAAAATCACTAAATAAATCATATCCAAAAGTACTTTCCTTTTTATTTTCCAATAAAATTAATATGTATTTATTTATGTTTAGCTACAGTTGGTTTGGGTTGATGATATCCACATATGTATTTTTAATTTCCACACTACCGGTTAATTTATATCCCTTAATCTTATTCAGTTCATTTGATCCAACAATCGAAATTAAAAACACACCACACTTCTTCAACCGCTGCCTTCAGCGATATTTCCCTCCATCTATTGTAAGAACTTGGATTGAATGTAAAGGAGGATGCTTCAGTCATGCAGTAAACCATGAACATCATTTGAAATGGTCAAATAAAATAACTGCACTTGCACTCTCCATCTCATTTAGTTTCTGCATCTCCACTTCAATTTTTTTTTTTTTTTTTGTGCTTCATCCCCATTTAAATACTCGATTCTCCTTTCTCCTATTAATATTATTCTCGTTTCTACAAAAATTCATAGAACAAAAAGATCACATGATCGATCCAGTTATTACTTGTAAGATCATGCGGCATGTTGATGTAATTTCAGAAATTCACATATTTATGACTTTCTCTTTCTCCTTTTTAAAAGGAAAAATGATTAAGTTAAGAAGATGTGGTACCTTGGACAAATCGAACAAAAATACGAAATGCACAGATGATGTCTCGGTCTGGTGCTGTCCAATGGTCCCCCAACACACATCGATCCATAATCTGGATCCATTCAGAAAAGAGCGTTCCAGTGCTGGCAAGGTTACTTTTTTAGTAAAATCTTCTTTTTGCCGTTTCATTTGGTGGGTGTCGTTTTTGGAATTTTATTTAAAGGACTTGTTTAATATTTTTAGGCATCGAAGTATAAGAAGACAACTTAAATATAGCTATAATTCAGTTACACACATAACCCAACGAACTTACCTGCCTGAAAGACCGACGTTTCCAACCCTGACTGTTAACATAAGCATGATTATTAGAGGAAAAAATTTGTATTTTAGCAGTGTTTCTGTTGTAGTTGTTGCACATTCTATCCAGCTGTACATGTTGTTTATACCTAAATGCGGTAAAGGCTTGCTTACCTGGCCACGCGGTGAAATGAGTGGACCTCAATTGAAACCCCAACTTTATAACAGAAGCCCTAACTTATTCCCCCTCGCAAGAAAAGCATATATACAGCACCCTAAAACTCGAACAAGTTGTACGATGGCTAAAACTTTGAAGTCAATTTGCCTTTCTGTGCTTTTTGTTATATTCATCGTCTTTGCAGTTGCAGGTGAGAATGAAGGTGTTTCTATGTGTATTGGAAATAATTACATAGTTGATATATATATATATATATATATATATATATATATTTATCTTACTTTCATATGTTTTAAGTACACAGAAAAATCTCTAGTAAACTCCATTGACCCTTTCTCTTCATTGTTCCTTATTTCTTTTATTTCGATCTTCAAATATGACTATATTATGTACGTACTTGGCACGTATTACATTCGACTAACTACTTCTATACTGTGTAAATTACTTACGAAGGTTTCATACGTAATGTTCGCTACTCGTATTTATAGGAAGCTAAGCAATGTAAAAAGACTGTGTAAGACTGTAATTGTACATCATATTTCCTTCTGCTTGTGGTGTCAATTGACATATAAGTGCACTCTAACCTATTTGTGCACTTACATTTTTAATGAAAAATTCATCTTAATCTCGATTACCCACCCACTTAAAACACAACACACTACTTACAAAATTTTCTCTCCTTATCTCACACATTTAGATGGCTACAGCAGTTTTGGCTTACAAATAATTCTTTTCTTAAATAGTGACATCCTTACTAAGGAAAACTTGTATATTCATGAGAACATTAAATATATTATAGTTTCACATTTTAATAAAAAGAAAATTCATTTTTTTTTAATTAGTATTTTGAATGTTCACCACCTTACGCCTTTTCTCTGTACTATAATTTCAAGTGACCGATGTCAAAAAACTATTTTAGTTACTCAGTTTAACTTTTTATTTTTAACAACACTCTCTATATCAACAAACTTTAATTATTTTATTGTTAACAATTCAAACCCAACCTACCTCTTCCTTTTTTTCCTTGGTCGAGGGTCTACCCTTGAATTTTATTATTTTTTATATTTACTTGTTTTATATAGTCGAGTTTTGATTGTTTCGCAGCATTCTTGCAAATGCTCTAATAACTTCCAACATTACACCTGTGGTAAAAATTCTTCTTGTACACACCCTTTGTTAAAAAAAGAAATTTATCACTACCCAACAGTGCACATTCACAACATGGGTTCAAGATCCTCTCAATATCTAAAAGTATGGAGCCTAATTACTCTAAAATTCAAGCCGTTCAAGTTTAGGAGTTCTTTTGAGATAAACCGGTATAATGGGTTAAAAGATCGTATCAGTCAAAACTATTTACGTGGCACAAATATGACTGAGAAGTTTGAAGGTGGGCAAAAATTGAAGGGTAGGAATACAAAGAACGTGAATTCGAATCTCGTGTTGCGTAAAAAGCGAGAAACAAAAGAGATGGAAATTCAAAAAGAGCGAGTGAGAGGGATGGCGAGAGATAAAGAAAGAGGCAGAATATGTCATGTCATCAGATACTCCGTCCGTTCAGTTTCCTCACGCTTTCCCTCTCACCTTCTCTTTTGCAAAACGCGGGACCCACCGTCTCTGTTCTATCGAAGCTTCTGGATTTTATTCCACCCAAAAAGCAAAAGTTCCCGGGGGGGGAATTTCGTCATTCTAACCTCTCCGCGCAAAGCAATTGCGCGAGGGCAAGACCCCGCCAAACAGAGGCCCTCATTTGTTGGTTTTTGTGGGTTTTTGAAGAAGAAAGCGAGTAGGAAGTTGAGTCGAGTTCTCTCTTTGATTTGTGCGTTTGCTCCCGATTTTCCAATGGAACGAGGCGGCGGCGGTGGATTTAGGGGAAGGGGAAGGGGAAGAGGGAGAGGGAGAGGGAGTGATGGTGCTGGTGGTGGAAGAGGTGATCCTGGCGGCTATGGCAGAGGAACTGGAGGGAGACGTGGAGGAGGAGGAGGAGAGAGAGGAAGAGGCGGTCATGACGGCGGTGTTAATGTTGGCGGGCCACAGTTCCACCACCATCATCCGCCGCAACAGCAGCCACAGCCGCCACTGCAACAGCAGCCACAGCAACAGCAACCGCCGAATCAGAATCCGAGTCAACAACCGAGGCCTAATCCATGGAATGCTCCCGCTTCTACCAGAGCATGGGGGCCCAGAGCGCCTGCTCCGCCTGCATCTCCGGTCGAATCGGCCCCGAACATCTCTCCTGATCCTGGTACTCTTGGTCCTGCAATGCAATCAATGGCAATTTCAGGACAGACACCCCCTTCATCTTCTTTGGAAAAGGCGAGCAATCATGTGCCAGTGAAGCGGCCTGATCGTGGGGGGATGATACATATCCGAACTGCTAGGCTCCGCGCTAATCATTTTAATCTCAAGTTCAATCCTGAGAGTATTATAATGCACTATGATGTTGATGTTAAGCCAGAACACCCTGCTAAGAATGGCCGTCCGGTGAAGATGTCAAAGTCTGAACTTGCTGCAATCAGGAAGCAGTTATCCACGGATGATCCTTCGAATTTTCCGTTGTTAAGTACAGCATATGATGGTGAAAAGAACATATTCAGTGCAGTGTCGTTGCCCACTGGATCATTTAAGGTGGAAGTTCCTGAGGAAGAAGGCACGCGGTTCAGTTCGTACATTTTGACTATAAAGCTTGCGAAGGAGCTGCAGCTTTGCAAGTTGAAGGAGTACCTAAGTGGTCAGCTGTTGGATATCCCTCGTAACATATTGCAGAGTATGGACTTGGTGATGAAAGAGAATCCAACTAGGCACATGATTGCTTTTGGGCGAAACTTTTACCCCACTGAATCTCATCCAAACGATGACCTTGGATATGGTGTTGCTGCCTTCAGAGGCTTTCAGCATGGCTTGAAGCTCACTTCTCAGGGTCCTACCTTGTGTCTGGATTACTCAGTCTTGGCATTTCATAAGCGCATGTCAGTTATTGATTTTCTCCAACAGCAAATAAGGGGATTTACTTTAAATAATTTCCAGAGTTATAGGAGAGATGTTGAGGATGTATTGAAGGGATTGCAAGTTAACGTTACTCACCGCAGAACCAAGCAGAAGTACCTCATTAAGGGGCTTACTGATAGGAATGCAGGAGATATTACATTTGATGCTGTAGACATAGATGGCCAGAGTCCACCTACGAAAGTTAGACTTCTTGATTATTTCAGGGACAAATACGAGGAGATTCGGTACAAAAACATTCCTTGCTTGGATTTGGGGAAAAATGGTAGAAGGAATGATACCCCAATGGAGTTCTGTGTCTTAGTTGAGGGGCAAAGGTATCCAAAGGAGTATTTGGGCAAAGAAGCAGCCATCATGCTGAAGAACATCTCATTGCCTTCACCAAGAGATAGACAGAATCGTATATACGACATGGTACGATCAAATGATGGACCTTGCGGTGGTGGCATCATTCAGAATTTTGGATTTGAAGTCAACCAGAATATGACACCAGTAACAGGGCGTGTGATAGGCCCTCCTGAGTTGAAGTTGGGTGCTTCTGATGGCAAGGTGGTGACTAAGGTGACTGTTGACAGAGAGAAATGCCAATGGAATCTGGTTGGGAAGAGAGTCGTGGAAGGAAAACCAATCATGCATTGGGCTGTACTAGACTTCAGCAGTTATGATCGTTATGGACTGGACCCTAACCAGTTCATCCCAAAGCTCATCACTCGGTGCAACAAACTGGGAATCAGAATGGAAGAGCCTCTTTTGTATGAACCCACTTCTATGAGGCCATTCTCTAGTGTTCAGATGCTTCGTCAGCTGCTTGAAAAGGTTAATGAAAAGGCCAAAAAAGAAGGCAAAGGTTACTTGCAACTACTTGTTTGTGTAATGGCAAATAGAGACCCTGGTTACAAGTATCTGAAGTGGATCGCTGAGACTCAAATTGGTATAGTGACACAGTGCTGCTTGTCAAATATGGCCAACAAAGCAAGTGACCAGTACCTTGCAAATCTTGCACTCAAGATCAATGCCAAGCTTGGAGGCAGTAATGTAGAGCTAATCGATCGGCTCCCCCTTTTAGGGGGTGCAGGCCATGTTATGTTTGTGGGGGCTGATGTCAATCATCCTGCTGCGCGAAACACAACAAGTCCATCAATTGCAGCTGTTGTTGCCACTGTAAACTGGCCTGCTGCAAATCGTTATGCTGCACGAGTTCGACCCCAGTACCATCGTAAGGAGAAGATAATAAATTTTGGAGACATGTGTCTCGAGCTTGTTCAAACTTACGAGCGGTTGAATAAAACCAAGCCAAACCAAATTGTTATCTTCCGTGATGGAGTCAGTGAGGGCCAGTTTGATATGGTTCTCAATGAAGAGTTACTAGATCTGAAGTTGGCTTTGGCAAGTATAAAATACCAACCAACCATCACACTTATTGTTGCCCAGAAGCGGCACCATACTCGTCTGTTTTTGGAGAATCCAAGGGATGGGGGTTCCACAGGCAATATATCACCGGGAACTGTTGTGGACACCACAATTGTGCACCCGTTTGAGTTTGACTTCTATCTCTGCAGTCATTATGGAGCCCTTGGGACAAGCAAACCCACGCACTACCATGTTCTTTGGGACGAGCACCGGTTTACTTCTGACCAATTGCAGAAGCTCATATATGACTTGTGCTTCACTTTTGCAAGGTGTACCAAACCTGTATCATTGGTCCCACCGGTGTACTATGCTGACCTCGTGGCATATAGGGGGCGATTATACTTTGAGAGTACGGAGGGACAGTCTCCAGCGTCAGTATCCTCCGCCTCCTCCTCATCCTCGACATATTCACGGCTGTCAGTGTCTTCTCTCGATGAAAGGTTTTGCAAGTTGCACGTTGAACTGGAGAACATAATGTTTTTCGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0016032 GO:0019048 GO:0035197 GO:0035821 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0061980 GO:0097159 GO:0098542 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

2348

Amino Acids

268.2

Weight (kDa)

9.69

Isoelectric Point (pI)

44.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	1505 - 1641	3.6e-22	N-terminal domain of argonaute
ArgoL1	PF08699	1652 - 1701	1.1e-08	Argonaute linker 1 domain
PAZ	PF02170	1720 - 1827	5.5e-20	PAZ domain
ArgoL2	PF16488	1845 - 1891	7.2e-06	Argonaute linker 2 domain
Piwi	PF02171	2003 - 2297	2.6e-93	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31280 AT1G31290 AT1G31290 AT1G31290
fragaria_vesca	FvH4_3g11980
malus_domestica	MD05G1256100.v1.1 MD10G1236300.v1.1 MD10G1236400.v1.1
prunus_persica	Prupe.4G108000_v2.0.a1 Prupe.4G108100_v2.0.a1
pyrus_communis	pycom05g23500 pycom05g23510 pycom05g23520 pycom10g19770 pycom10g19780
rosa_chinensis	RchiOBHm_Chr5g0019581
rosa_laevigata	RLG00000032457
rosa_roxburghii	Rroxscaffold_1G00058500
rosa_rugosa	Rorug05G0052100 Rorug05G0052200
rosa_samantha	Rh5AG142700 Rh5BG141200 Rh5CG153300 Rh5DG141900
rosa_wichuraiana	Rw5G012630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2187, 4568
AarI	CACCTGC	2 cut(s)	196, 2321
Acc36I	ACCTGC	3 cut(s)	196, 1989, 2321
Acc65I	GGTACC	1 cut(s)	1685
AccB1I	GGYRCC	2 cut(s)	1685, 6582
AccB7I	CCANNNNNTGG	3 cut(s)	1780, 4314, 6617
AccBSI	CCGCTC	1 cut(s)	6421
AccI	GTMKAC	2 cut(s)	3179, 5298
AccII	CGCG	8 cut(s)	579, 2148, 3742, 3841, 3856, 4527, 4795, 6265
AclI	AACGTT	1 cut(s)	5214
AcoI	YGGCCR	4 cut(s)	2141, 4615, 5308, 6108
AcuI	CTGAAG	8 cut(s)	473, 1280, 1339, 5005, 5525, 5782, 6071, 6539
AdeI	CACNNNGTG	2 cut(s)	2150, 5859
AflIII	ACRYGT	2 cut(s)	2100, 6390
AgeI	ACCGGT	4 cut(s)	1207, 3456, 6765, 6853
AhdI	GACNNNNNGTC	2 cut(s)	586, 3751
AjiI	CACGTC	1 cut(s)	4109
AjnI	CCWGG	6 cut(s)	596, 647, 2138, 4075, 4381, 6034
AjuI	GAANNNNNNNTTGG	4 cut(s)	1087, 1119, 6512, 6544
AleI	CACNNNNGTG	1 cut(s)	6668
Alw21I	GWGCWC	6 cut(s)	639, 2720, 2738, 3354, 6675, 6765
Alw26I	GTCTC	9 cut(s)	150, 1735, 3759, 4099, 6024, 6059, 6381, 6401, 6924
Alw44I	GTGCAC	5 cut(s)	635, 2716, 2734, 3350, 6671
Ama87I	CYCGRG	2 cut(s)	3807, 6398
ApaI	GGGCCC	1 cut(s)	4323
ApaLI	GTGCAC	5 cut(s)	635, 2716, 2734, 3350, 6671
AseI	ATTAAT	2 cut(s)	1128, 1514
AsiGI	ACCGGT	4 cut(s)	1207, 3456, 6765, 6853
Asp700I	GAANNNNTTC	1 cut(s)	4730
Asp718I	GGTACC	1 cut(s)	1685
AspA2I	CCTAGG	1 cut(s)	808
AspLEI	GCGC	6 cut(s)	3843, 3856, 4330, 4529, 5106, 6265
AsuC2I	CCSGG	3 cut(s)	3808, 3809, 6645
AsuII	TTCGAA	2 cut(s)	3572, 4685
AvaI	CYCGRG	2 cut(s)	3807, 6398
AvaII	GGWCC	9 cut(s)	652, 1753, 2162, 3745, 4394, 5060, 5576, 5824, 6847
AvrII	CCTAGG	1 cut(s)	808
AxyI	CCTNAGG	1 cut(s)	4779
BaeGI	GKGCMC	6 cut(s)	639, 2720, 2738, 3354, 4323, 6675
BaeI	ACNNNNGTAYC	2 cut(s)	6338, 6371
BalI	TGGCCA	3 cut(s)	2143, 5310, 6110
BamHI	GGATCC	1 cut(s)	1783
BanI	GGYRCC	2 cut(s)	1685, 6582
BanII	GRGCYC	2 cut(s)	4323, 6718
BarI	GAAGNNNNNNTAC	8 cut(s)	1669, 1701, 2585, 2617, 3283, 3315, 5407, 5439
BauI	CACGAG	3 cut(s)	3578, 6339, 6872
BbsI	GAAGAC	3 cut(s)	940, 1933, 6972
Bbv12I	GWGCWC	6 cut(s)	639, 2720, 2738, 3354, 6675, 6765
BceAI	ACGGC	5 cut(s)	1833, 3409, 4165, 4602, 6983
BcgI	CGANNNNNNTGC	4 cut(s)	4819, 4853, 6572, 6606
BciT130I	CCWGG	6 cut(s)	598, 649, 2140, 4077, 4383, 6036
BciVI	GTATCC	2 cut(s)	5475, 6943
BcnI	CCSGG	3 cut(s)	3808, 3809, 6645
BcoDI	GTCTC	9 cut(s)	150, 1735, 3759, 4099, 6024, 6059, 6381, 6401, 6924
BfaI	CTAG	9 cut(s)	153, 809, 1040, 2456, 4518, 4938, 5792, 5928, 6512
BfmI	CTRYAG	5 cut(s)	1153, 2845, 4838, 5295, 6700
BfoI	RGCGCY	1 cut(s)	4331
BfuAI	ACCTGC	3 cut(s)	196, 1989, 2321
BfuI	GTATCC	2 cut(s)	5475, 6943
BglII	AGATCT	1 cut(s)	6514
BlnI	CCTAGG	1 cut(s)	808
BlpI	GCTNAGC	1 cut(s)	2636
BmcAI	AGTACT	1 cut(s)	1101
Bme1390I	CCNGG	9 cut(s)	598, 649, 2140, 3808, 3809, 4077, 4383, 6036, 6645
Bme18I	GGWCC	9 cut(s)	652, 1753, 2162, 3745, 4394, 5060, 5576, 5824, 6847
BmeRI	GACNNNNNGTC	2 cut(s)	586, 3751
BmeT110I	CYCGRG	2 cut(s)	3807, 6398
BmgBI	CACGTC	1 cut(s)	4109
BmrFI	CCNGG	9 cut(s)	598, 649, 2140, 3808, 3809, 4077, 4383, 6036, 6645
BmrI	ACTGGG	2 cut(s)	5879, 6346
BmuI	ACTGGG	2 cut(s)	5879, 6346
BoxI	GACNNNNGTC	2 cut(s)	3042, 6393
BpiI	GAAGAC	3 cut(s)	940, 1933, 6972
BplI	GAGNNNNNCTC	2 cut(s)	2149, 2181
BpmI	CTGGAG	3 cut(s)	4119, 6906, 7040
Bpu1102I	GCTNAGC	1 cut(s)	2636
Bpu14I	TTCGAA	2 cut(s)	3572, 4685
BpuEI	CTTGAG	2 cut(s)	4529, 6134
BpuMI	CCSGG	3 cut(s)	3808, 3809, 6645
Bsa29I	ATCGAT	3 cut(s)	1566, 1769, 6192
BsaAI	YACGTR	4 cut(s)	2536, 2547, 2605, 3500
BsaBI	GATNNNNATC	2 cut(s)	4505, 6249
BsaI	GGTCTC	1 cut(s)	6024
BsaWI	WCCGGW	6 cut(s)	1207, 3456, 4348, 4621, 6765, 6853
BsaXI	ACNNNNNCTCC	8 cut(s)	4030, 4036, 4060, 4066, 5357, 5387, 6902, 6932
Bse118I	RCCGGY	4 cut(s)	1207, 3456, 6765, 6853
Bse21I	CCTNAGG	1 cut(s)	4779
Bse3DI	GCAATG	3 cut(s)	2647, 4407, 5516
Bse8I	GATNNNNATC	2 cut(s)	4505, 6249
BseBI	CCWGG	6 cut(s)	598, 649, 2140, 4077, 4383, 6036
BseCI	ATCGAT	3 cut(s)	1566, 1769, 6192
BseJI	GATNNNNATC	2 cut(s)	4505, 6249
BseMI	GCAATG	3 cut(s)	2647, 4407, 5516
BseMII	CTCAG	8 cut(s)	3080, 3507, 4548, 4770, 5069, 5097, 5655, 6054
BseRI	GAGGAG	8 cut(s)	300, 4128, 4131, 4134, 4137, 5378, 6934, 6937
BseSI	GKGCMC	6 cut(s)	639, 2720, 2738, 3354, 4323, 6675
BsgI	GTGCAG	5 cut(s)	174, 1392, 4758, 6237, 6321
Bsh1236I	CGCG	8 cut(s)	579, 2148, 3742, 3841, 3856, 4527, 4795, 6265
Bsh1285I	CGRYCG	2 cut(s)	4353, 6196
BshNI	GGYRCC	2 cut(s)	1685, 6582
BshTI	ACCGGT	4 cut(s)	1207, 3456, 6765, 6853
BshVI	ATCGAT	3 cut(s)	1566, 1769, 6192
BsiEI	CGRYCG	2 cut(s)	4353, 6196
BsiHKAI	GWGCWC	6 cut(s)	639, 2720, 2738, 3354, 6675, 6765
BsiHKCI	CYCGRG	2 cut(s)	3807, 6398
BsiSI	CCGG	8 cut(s)	1208, 3457, 3808, 4349, 4622, 6644, 6766, 6854
BsmAI	GTCTC	9 cut(s)	150, 1735, 3759, 4099, 6024, 6059, 6381, 6401, 6924
BsmBI	CGTCTC	2 cut(s)	3759, 4099
BsmI	GAATGC	3 cut(s)	3247, 4297, 5275
Bso31I	GGTCTC	1 cut(s)	6024
BsoBI	CYCGRG	2 cut(s)	3807, 6398
Bsp119I	TTCGAA	2 cut(s)	3572, 4685
Bsp120I	GGGCCC	1 cut(s)	4319
Bsp1286I	GDGCHC	8 cut(s)	639, 2720, 2738, 3354, 4323, 6675, 6718, 6765
Bsp1407I	TGTACA	3 cut(s)	2097, 2671, 3305
Bsp1720I	GCTNAGC	1 cut(s)	2636
Bsp19I	CCATGG	2 cut(s)	657, 4286
BspCNI	CTCAG	8 cut(s)	3079, 3508, 4549, 4771, 5068, 5096, 5656, 6055
BspDI	ATCGAT	3 cut(s)	1566, 1769, 6192
BspFNI	CGCG	8 cut(s)	579, 2148, 3742, 3841, 3856, 4527, 4795, 6265
BspHI	TCATGA	2 cut(s)	2917, 4144
BspMAI	CTGCAG	2 cut(s)	4842, 6704
BspMI	ACCTGC	3 cut(s)	196, 1989, 2321
BspQI	GCTCTTC	1 cut(s)	5880
BspT104I	TTCGAA	2 cut(s)	3572, 4685
BspT107I	GGYRCC	2 cut(s)	1685, 6582
BspTNI	GGTCTC	1 cut(s)	6024
BsrBI	CCGCTC	1 cut(s)	6421
BsrDI	GCAATG	3 cut(s)	2647, 4407, 5516
BsrFI	RCCGGY	4 cut(s)	1207, 3456, 6765, 6853
BsrGI	TGTACA	3 cut(s)	2097, 2671, 3305
BssAI	RCCGGY	4 cut(s)	1207, 3456, 6765, 6853
BssSI	CACGAG	3 cut(s)	3578, 6339, 6872
BssT1I	CCWWGG	8 cut(s)	657, 808, 1689, 3166, 4286, 4999, 6611, 6718
Bst2BI	CACGAG	3 cut(s)	3578, 6339, 6872
Bst2UI	CCWGG	6 cut(s)	598, 649, 2140, 4077, 4383, 6036
BstAPI	GCANNNNNTGC	1 cut(s)	6143
BstAUI	TGTACA	3 cut(s)	2097, 2671, 3305
BstBAI	YACGTR	4 cut(s)	2536, 2547, 2605, 3500
BstBI	TTCGAA	2 cut(s)	3572, 4685
BstDSI	CCRYGG	3 cut(s)	657, 4286, 4671
BstENI	CCTNNNNNAGG	2 cut(s)	2897, 5249
BstFNI	CGCG	8 cut(s)	579, 2148, 3742, 3841, 3856, 4527, 4795, 6265
BstH2I	RGCGCY	1 cut(s)	4331
BstHHI	GCGC	6 cut(s)	3843, 3856, 4330, 4529, 5106, 6265
BstMAI	GTCTC	9 cut(s)	150, 1735, 3759, 4099, 6024, 6059, 6381, 6401, 6924
BstMCI	CGRYCG	2 cut(s)	4353, 6196
BstNI	CCWGG	6 cut(s)	598, 649, 2140, 4077, 4383, 6036
BstNSI	RCATGY	4 cut(s)	1601, 2104, 5110, 6394
BstPAI	GACNNNNGTC	2 cut(s)	3042, 6393
BstSCI	CCNGG	9 cut(s)	596, 647, 2138, 3806, 3807, 4075, 4381, 6034, 6643
BstSFI	CTRYAG	5 cut(s)	1153, 2845, 4838, 5295, 6700
BstSLI	GKGCMC	6 cut(s)	639, 2720, 2738, 3354, 4323, 6675
BstSNI	TACGTA	2 cut(s)	2536, 2605
BstUI	CGCG	8 cut(s)	579, 2148, 3742, 3841, 3856, 4527, 4795, 6265
BstV2I	GAAGAC	3 cut(s)	940, 1933, 6972
BstX2I	RGATCY	6 cut(s)	164, 484, 523, 1783, 3374, 6514
BstYI	RGATCY	6 cut(s)	164, 484, 523, 1783, 3374, 6514
Bsu15I	ATCGAT	3 cut(s)	1566, 1769, 6192
Bsu36I	CCTNAGG	1 cut(s)	4779
BsuI	GTATCC	2 cut(s)	5475, 6943
BsuTUI	ATCGAT	3 cut(s)	1566, 1769, 6192
BtgI	CCRYGG	3 cut(s)	657, 4286, 4671
BtrI	CACGTC	1 cut(s)	4109
BtsI	GCAGTG	3 cut(s)	2065, 4217, 4746
BveI	ACCTGC	3 cut(s)	196, 1989, 2321
CciI	TCATGA	2 cut(s)	2917, 4144
CfoI	GCGC	6 cut(s)	3843, 3856, 4330, 4529, 5106, 6265
Cfr10I	RCCGGY	4 cut(s)	1207, 3456, 6765, 6853
Cfr9I	CCCGGG	1 cut(s)	3807
ClaI	ATCGAT	3 cut(s)	1566, 1769, 6192
CseI	GACGC	2 cut(s)	697, 6915
CspAI	ACCGGT	4 cut(s)	1207, 3456, 6765, 6853
DraI	TTTAAA	4 cut(s)	1488, 1653, 1887, 5154
DraIII	CACNNNGTG	2 cut(s)	2150, 5859
DriI	GACNNNNNGTC	2 cut(s)	586, 3751
EaeI	YGGCCR	4 cut(s)	2141, 4615, 5308, 6108
Eam1105I	GACNNNNNGTC	2 cut(s)	586, 3751
EciI	GGCGGA	4 cut(s)	700, 4182, 4326, 6928
Eco105I	TACGTA	2 cut(s)	2536, 2605
Eco130I	CCWWGG	8 cut(s)	657, 808, 1689, 3166, 4286, 4999, 6611, 6718
Eco147I	AGGCCT	1 cut(s)	4279
Eco24I	GRGCYC	2 cut(s)	4323, 6718
Eco31I	GGTCTC	1 cut(s)	6024
Eco32I	GATATC	2 cut(s)	1176, 4885
Eco47I	GGWCC	9 cut(s)	652, 1753, 2162, 3745, 4394, 5060, 5576, 5824, 6847
Eco57I	CTGAAG	8 cut(s)	473, 1280, 1339, 5005, 5525, 5782, 6071, 6539
Eco81I	CCTNAGG	1 cut(s)	4779
Eco88I	CYCGRG	2 cut(s)	3807, 6398
EcoNI	CCTNNNNNAGG	2 cut(s)	2897, 5249
EcoO109I	RGGNCCY	6 cut(s)	652, 3745, 3882, 4319, 5060, 5657
EcoRI	GAATTC	1 cut(s)	3568
EcoRII	CCWGG	6 cut(s)	596, 647, 2138, 4075, 4381, 6034
EcoRV	GATATC	2 cut(s)	1176, 4885
EcoT14I	CCWWGG	8 cut(s)	657, 808, 1689, 3166, 4286, 4999, 6611, 6718
EcoT22I	ATGCAT	1 cut(s)	5781
EcoT38I	GRGCYC	2 cut(s)	4323, 6718
ErhI	CCWWGG	8 cut(s)	657, 808, 1689, 3166, 4286, 4999, 6611, 6718
Esp3I	CGTCTC	2 cut(s)	3759, 4099
FalI	AAGNNNNNCTT	8 cut(s)	2118, 2150, 2891, 2923, 5319, 5351, 5877, 5909
FauI	CCCGC	4 cut(s)	3735, 3877, 4160, 4306
FauNDI	CATATG	3 cut(s)	1183, 2429, 4711
FblI	GTMKAC	2 cut(s)	3179, 5298
FriOI	GRGCYC	2 cut(s)	4323, 6718
FspBI	CTAG	9 cut(s)	153, 809, 1040, 2456, 4518, 4938, 5792, 5928, 6512
GlaI	GCGC	6 cut(s)	3842, 3855, 4329, 4528, 5105, 6264
GsuI	CTGGAG	3 cut(s)	4119, 6906, 7040
HaeII	RGCGCY	1 cut(s)	4331
HapII	CCGG	8 cut(s)	1208, 3457, 3808, 4349, 4622, 6644, 6766, 6854
HgaI	GACGC	2 cut(s)	697, 6915
HhaI	GCGC	6 cut(s)	3843, 3856, 4330, 4529, 5106, 6265
Hin6I	GCGC	6 cut(s)	3841, 3854, 4328, 4527, 5104, 6263
HinP1I	GCGC	6 cut(s)	3841, 3854, 4328, 4527, 5104, 6263
HincII	GTYRAC	6 cut(s)	2017, 3130, 4268, 5212, 5620, 5716
HindII	GTYRAC	6 cut(s)	2017, 3130, 4268, 5212, 5620, 5716
HindIII	AAGCTT	3 cut(s)	3769, 4823, 6164
HpaI	GTTAAC	3 cut(s)	2017, 3130, 5212
HpaII	CCGG	8 cut(s)	1208, 3457, 3808, 4349, 4622, 6644, 6766, 6854
Hpy99I	CGWCG	1 cut(s)	1999
HpyCH4IV	ACGT	9 cut(s)	1997, 2535, 2546, 2604, 3499, 3564, 4108, 5214, 7011
HpySE526I	ACGT	9 cut(s)	1997, 2535, 2546, 2604, 3499, 3564, 4108, 5214, 7011
HspAI	GCGC	6 cut(s)	3841, 3854, 4328, 4527, 5104, 6263
KflI	GGGWCCC	2 cut(s)	652, 3745
KpnI	GGTACC	1 cut(s)	1689
KspAI	GTTAAC	3 cut(s)	2017, 3130, 5212
LguI	GCTCTTC	1 cut(s)	5880
LmnI	GCTCC	8 cut(s)	3399, 3973, 4300, 4339, 4527, 4834, 6204, 6713
MaeI	CTAG	9 cut(s)	153, 809, 1040, 2456, 4518, 4938, 5792, 5928, 6512
MaeII	ACGT	9 cut(s)	1997, 2535, 2546, 2604, 3499, 3564, 4108, 5214, 7011
MbiI	CCGCTC	1 cut(s)	6421
MfeI	CAATTG	8 cut(s)	556, 887, 2168, 2702, 3849, 6285, 6666, 6782
MflI	RGATCY	6 cut(s)	164, 484, 523, 1783, 3374, 6514
MhlI	GDGCHC	8 cut(s)	639, 2720, 2738, 3354, 4323, 6675, 6718, 6765
MlsI	TGGCCA	3 cut(s)	2143, 5310, 6110
MluNI	TGGCCA	3 cut(s)	2143, 5310, 6110
MlyI	GAGTC	6 cut(s)	3946, 4273, 5323, 5763, 6061, 6477
MmeI	TCCRAC	6 cut(s)	1274, 2027, 3298, 4860, 4958, 5152
Mox20I	TGGCCA	3 cut(s)	2143, 5310, 6110
Mph1103I	ATGCAT	1 cut(s)	5781
MroXI	GAANNNNTTC	1 cut(s)	4730
MscI	TGGCCA	3 cut(s)	2143, 5310, 6110
Msp20I	TGGCCA	3 cut(s)	2143, 5310, 6110
MspA1I	CMGCKG	5 cut(s)	1290, 2095, 4876, 5950, 6293
MspI	CCGG	8 cut(s)	1208, 3457, 3808, 4349, 4622, 6644, 6766, 6854
MspR9I	CCNGG	9 cut(s)	598, 649, 2140, 3808, 3809, 4077, 4383, 6036, 6645
MunI	CAATTG	8 cut(s)	556, 887, 2168, 2702, 3849, 6285, 6666, 6782
Mva1269I	GAATGC	3 cut(s)	3247, 4297, 5275
MvaI	CCWGG	6 cut(s)	598, 649, 2140, 4077, 4383, 6036
MvnI	CGCG	8 cut(s)	579, 2148, 3742, 3841, 3856, 4527, 4795, 6265
NciI	CCSGG	3 cut(s)	3808, 3809, 6645
NcoI	CCATGG	2 cut(s)	657, 4286
NdeI	CATATG	3 cut(s)	1183, 2429, 4711
NmeAIII	GCCGAG	1 cut(s)	677
NmuCI	GTSAC	7 cut(s)	61, 2885, 3035, 5698, 5707, 6082, 6122
NsiI	ATGCAT	1 cut(s)	5781
NspI	RCATGY	4 cut(s)	1601, 2104, 5110, 6394
NspV	TTCGAA	2 cut(s)	3572, 4685
OliI	CACNNNNGTG	1 cut(s)	6668
PaeR7I	CTCGAG	1 cut(s)	6398
PagI	TCATGA	2 cut(s)	2917, 4144
PaqCI	CACCTGC	2 cut(s)	196, 2321
PasI	CCCWGGG	1 cut(s)	597
PceI	AGGCCT	1 cut(s)	4279
PciI	ACATGT	2 cut(s)	2100, 6390
PciSI	GCTCTTC	1 cut(s)	5880
PcsI	WCGNNNNNNNCGW	2 cut(s)	704, 4691
PctI	GAATGC	3 cut(s)	3247, 4297, 5275
PdmI	GAANNNNTTC	1 cut(s)	4730
PflFI	GACNNNGTC	1 cut(s)	67
PflMI	CCANNNNNTGG	3 cut(s)	1780, 4314, 6617
PinAI	ACCGGT	4 cut(s)	1207, 3456, 6765, 6853
Ple19I	CGATCG	1 cut(s)	6196
PleI	GAGTC	6 cut(s)	3945, 4272, 5322, 5762, 6061, 6476
PpsI	GAGTC	6 cut(s)	3945, 4272, 5322, 5762, 6061, 6476
Ppu21I	YACGTR	4 cut(s)	2536, 2547, 2605, 3500
PpuMI	RGGWCCY	3 cut(s)	652, 3745, 5060
PscI	ACATGT	2 cut(s)	2100, 6390
PshAI	GACNNNNGTC	2 cut(s)	3042, 6393
PshBI	ATTAAT	2 cut(s)	1128, 1514
PsiI	TTATAA	2 cut(s)	2187, 4568
Psp1406I	AACGTT	1 cut(s)	5214
Psp5II	RGGWCCY	3 cut(s)	652, 3745, 5060
Psp6I	CCWGG	6 cut(s)	596, 647, 2138, 4075, 4381, 6034
PspGI	CCWGG	6 cut(s)	596, 647, 2138, 4075, 4381, 6034
PspOMI	GGGCCC	1 cut(s)	4319
PspPPI	RGGWCCY	3 cut(s)	652, 3745, 5060
PsrI	GAACNNNNNNTAC	2 cut(s)	2242, 2274
PstI	CTGCAG	2 cut(s)	4842, 6704
PsuI	RGATCY	6 cut(s)	164, 484, 523, 1783, 3374, 6514
PsyI	GACNNNGTC	1 cut(s)	67
PvuI	CGATCG	1 cut(s)	6196
PvuII	CAGCTG	4 cut(s)	2095, 4876, 5950, 6293
SapI	GCTCTTC	1 cut(s)	5880
ScaI	AGTACT	1 cut(s)	1101
SchI	GAGTC	6 cut(s)	3946, 4273, 5323, 5763, 6061, 6477
ScrFI	CCNGG	9 cut(s)	598, 649, 2140, 3808, 3809, 4077, 4383, 6036, 6645
SduI	GDGCHC	8 cut(s)	639, 2720, 2738, 3354, 4323, 6675, 6718, 6765
SfcI	CTRYAG	5 cut(s)	1153, 2845, 4838, 5295, 6700
Sfr274I	CTCGAG	1 cut(s)	6398
SfuI	TTCGAA	2 cut(s)	3572, 4685
SgrAI	CRCCGGYG	1 cut(s)	6853
SinI	GGWCC	9 cut(s)	652, 1753, 2162, 3745, 4394, 5060, 5576, 5824, 6847
SlaI	CTCGAG	1 cut(s)	6398
SmaI	CCCGGG	1 cut(s)	3809
SmiI	ATTTAAAT	1 cut(s)	1488
SmlI	CTYRAG	3 cut(s)	4544, 6149, 6398
SmoI	CTYRAG	3 cut(s)	4544, 6149, 6398
SnaBI	TACGTA	2 cut(s)	2536, 2605
SseBI	AGGCCT	1 cut(s)	4279
SspI	AATATT	3 cut(s)	848, 1518, 1903
SspMI	CTAG	9 cut(s)	153, 809, 1040, 2456, 4518, 4938, 5792, 5928, 6512
StuI	AGGCCT	1 cut(s)	4279
StyD4I	CCNGG	9 cut(s)	596, 647, 2138, 3806, 3807, 4075, 4381, 6034, 6643
StyI	CCWWGG	8 cut(s)	657, 808, 1689, 3166, 4286, 4999, 6611, 6718
SwaI	ATTTAAAT	1 cut(s)	1488
TaiI	ACGT	9 cut(s)	2000, 2538, 2549, 2607, 3502, 3567, 4111, 5217, 7014
TaqII	GACCGA	3 cut(s)	1723, 2008, 3054
TatI	WGTACW	9 cut(s)	1099, 2097, 2439, 2671, 3020, 3305, 4703, 5788, 6858
TseFI	GTSAC	7 cut(s)	61, 2885, 3035, 5698, 5707, 6082, 6122
Tsp45I	GTSAC	7 cut(s)	61, 2885, 3035, 5698, 5707, 6082, 6122
TspGWI	ACGGA	7 cut(s)	915, 3679, 3683, 4683, 4688, 6449, 6923
TspMI	CCCGGG	1 cut(s)	3807
Tth111I	GACNNNGTC	1 cut(s)	67
Van91I	CCANNNNNTGG	3 cut(s)	1780, 4314, 6617
VneI	GTGCAC	5 cut(s)	635, 2716, 2734, 3350, 6671
VpaK11BI	GGWCC	9 cut(s)	652, 1753, 2162, 3745, 4394, 5060, 5576, 5824, 6847
VspI	ATTAAT	2 cut(s)	1128, 1514
XagI	CCTNNNNNAGG	2 cut(s)	2897, 5249
XceI	RCATGY	4 cut(s)	1601, 2104, 5110, 6394
XcmI	CCANNNNNNNNNTGG	1 cut(s)	5778
XhoI	CTCGAG	1 cut(s)	6398
XmaI	CCCGGG	1 cut(s)	3807
XmaJI	CCTAGG	1 cut(s)	808
XmiI	GTMKAC	2 cut(s)	3179, 5298
XmnI	GAANNNNTTC	1 cut(s)	4730
XspI	CTAG	9 cut(s)	153, 809, 1040, 2456, 4518, 4938, 5792, 5928, 6512
ZrmI	AGTACT	1 cut(s)	1101
Zsp2I	ATGCAT	1 cut(s)	5781

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.