Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007187

Physical Location & Seq

Genomic Coordinates Forward (+)

33291888 .. 33295749

Gene Structure 3862 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1236300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3015 bp

ATGGAAAGAGGCGGCGGTAGCGGTGGATACAGAGGAAGGGGAATGGGAAGAGGGAGAGGCAGTGATGGTGGTGGTGGTGGAAGGGGTGATCAAGGCGGGTATGGCAGAGGAAATAGAGGGAGAGGTGGAGGAGGTGGAGGAGAGAGAGGAAGAGGCGGTCATGGTGGCGGGCCACAGTTCCACCACCATCAGCCACAACAGCAGCAACAGCAGCAACGACAGCCGAATCAGAATCGGAGTCAGCAAGCGAGGCCTAATCCATGGAATAGTCCCGCTCCTGCCAGAATATGGGGCCCCAGAGCGCCTGCTCCGCCTCCATCTCCGGCCGGACCGGCCCCGAACATCTCTCCTGCTCCTGATAATCTTGTTCCGGCAATGCAATCAATGGCAATTTCAAGACAGGCACCTGCTTCATCTTCTTTAGACAAGGCGGCCAATCTTGTCCAAGTAAAACGGCCTGATAATGGGGGCACAAAAGCTATCCGAACTGCTAGGCTTCGCGCTAATCATTTTAATCTCTCGTTCAATCCTGAAAGTATTATAATGCACTATGATGTTGATGTTAAACCGGAACACCCTGCTAAGAATGGCCTTCCTGTGAAGATATCAAAGTCTGAACTTTCTGCAATCAGGAACAAGTTATTCTCTGATGATCCTTCGAAATTTCCCTTGTTAAGTACCGCATATGATGGTGAAAAGAACATTTTCAGTGCAGTGCCGTTGCCCACTGGATCATTTAAGGTGGAGATTCCTGAGGAAGAAGGCACACGGTTCAGTTCGTACATTTTTACTATAAAGCTTGCGAAGGAGCTCCAGCTTCGCAAGTTGAAGGAGTACCTAAGCGGTCAGCTGTTGGATATCCCTCGTAATATATTGCAGAGTATGGATTTGGTTATGAAAGAGAATCCAGCTAGACGCATGATAGCTGTTGGGCGAAACTTTTACCCTACTGAATTTGATCCAAACGATGACCTTGGATATGGTGTTGCTGCCTTCAGAGGTTTTCAGCATAGCTTGAAGCTCACATCCCAGGGTCCTACCTTGTGTCTCGATTACTCAGTCTTGGCATTTCATAAGCGCATGTCAGTTACAGAATTTCTCCAAGAGCAGATAAGGGGTTTTACATTAAATGATTTCAAGAGGTTTAGGAGAGAGGTTGAGGATGCATTGAAGGGATTGAAAGTTACTGTGACTCACCGGAAAACCAAGCAAACGTACGTCATCATGGGGCTGACTGATAAGAATGCAGGAGAAATTACATTTGATGCTGTAGACATAGATGGCCAGAGTCCACCAAGGAAAGTTAGACTTCCTGATTATTTCAGGGACAAATACAGAGAGATACAGTACAAAAACATTCCCTGCTTGGATTTAGGGAAAAATGGTAAAAGGAATGATACACCAATGGAGTTCTGTGTCTTAGTTGAGGGGCAAAGGTATCCAAAGGAGTATTTGGGGAAAGAAGCAGCCGTCATGCTGAAGAACATCTCATTGCCTTCACCAAGAGACAGACAGAACATGATATCCAACATGGTACGATCAAATGATGGACCTTCCGGTGGTGGCATCATTCAAAATTTTGGATTTGAGGTCAACCAGAATCTGACACCAGTGACAGGGCGTGTGATTGTCCCACCTGAGTTGAAGTTGGGTGCTTCTGATGGCAAGGTGATTAAGGTGACTGTTGACAGAGAGAAATGTCAATGGAATCTGGTTGGGAAGACTCTTGTGGAAGGAAAACCAATCAAGTGCTGGGCCGTGCTTGACTTCAGCAGTTCTGATCGTTTCAAACTGGACCCTAACCAGTTCATCTCAAAGCTCATCGCTCGGTGCAACAGACTGGGAATCAGAATGGGAGAACCTGTTTTGTATGAAACCACTTCAATGAGGCCATTCTCTAGTGTTCAGATGCTTCGTCCACTGCTTGAAAGCGTCTATGAAAAGGCAAAAAAAGAAGGCAATGGCTACTTGCAGTTACTTGTTTGTGTAATGTCTAAAAGAGACCCTGGTTACAAGCATCTGAAGTGGATTGCTGAGACTCAAATTGGTATAGTGACGCAGTGCTGCTTGTCAAATATGGCCAACAAAGCAAATGACCAGTACCTTGCAAATCTTGCACTCAAGATCAATGCCAAGCTTGGAGGCAGTAATGTAGAGCTAAATGATCGGCTCCCCCTTGTAGGGGGTGCAGGCCATGTTATGTTTGTGGGGGCTGATGTCAATCATCCTGCCGCGCGAAACACAACAAGTCCATCAATTGCAGCAGTTGTTGCCACTGTAAACTGGCCTGCTGCGAATCGTTATGCTGCACGAGTTCGATCCCAGTACCATCGTGTGGAGAAGATAATGAAATTTGGAGACATGTGTCTGGAGCTTGTTGAATCTTATGAGCGATTGAATAAAGTCAAGCCAGAGAAAATTATTATCTTCCGTGATGGAGTCAGTGAGGGCCAGTTTGATATGGTTCTCAACGAAGAGTTGCTAGATCTGAAGATGGCTTTTGAAAGTATAAAATACCATCCAACCATCACACTTATTGTTGCCCAGAAGCGGCACCATACTCGTCTGTTTCTGGAGAATCCAAGGGATGGGGGTTCCGCAGGCAATATATCACCGGGAACTGTTGTGGACACAACAATCGTGCACCCGTTTGAGTTTGACTTCTATCTTTGCAGTCATTACGGAGCCCTTGGAACAAGCAAACCCACACACTACCATGTTCTTTGGGACGAGCATCGGTTTACCTCAGATCAATTACAGAAGCTTATATATGACTTGTGCTTCACCTTTGCAAGATGTACAAAACCTGTATCATTGATCCCACCGGTGTACTATGCCGACCTCGTGGCATATAGGGGGCGATTATACTACGAGAGTACGGAGGGACAGTCTCCAGCTTCAGTATCCTCCTCCTCGTCCTCCTCCTCGACACATTCACCGCTGTCAGCGGCTTCTCTTGATGAAAGGTTCTGCAAACTGCACGCTGACTTGGAAAACATAATGTTTTTCGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0016032 GO:0019048 GO:0035197 GO:0035821 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0061980 GO:0097159 GO:0098542 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

1005

Amino Acids

111.57

Weight (kDa)

9.39

Isoelectric Point (pI)

43.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	163 - 299	9.3e-23	N-terminal domain of argonaute
ArgoL1	PF08699	309 - 359	2.1e-11	Argonaute linker 1 domain
PAZ	PF02170	379 - 486	1.1e-21	PAZ domain
ArgoL2	PF16488	503 - 549	3.3e-06	Argonaute linker 2 domain
Piwi	PF02171	660 - 956	2.3e-93	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31280 AT1G31290 AT1G31290 AT1G31290
fragaria_vesca	FvH4_3g11980
malus_domestica	MD05G1256100.v1.1 MD10G1236300.v1.1 MD10G1236400.v1.1
prunus_persica	Prupe.4G108000_v2.0.a1 Prupe.4G108100_v2.0.a1
pyrus_communis	pycom05g23500 pycom05g23510 pycom05g23520 pycom10g19770 pycom10g19780
rosa_chinensis	RchiOBHm_Chr5g0019581
rosa_laevigata	RLG00000032457
rosa_roxburghii	Rroxscaffold_1G00058500
rosa_rugosa	Rorug05G0052100 Rorug05G0052200
rosa_samantha	Rh5AG142700 Rh5BG141200 Rh5CG153300 Rh5DG141900
rosa_wichuraiana	Rw5G012630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	542
AarI	CACCTGC	1 cut(s)	415
Acc36I	ACCTGC	1 cut(s)	415
AccB1I	GGYRCC	2 cut(s)	403, 2553
AccB7I	CCANNNNNTGG	3 cut(s)	288, 2335, 2588
AccBSI	CCGCTC	1 cut(s)	275
AccI	GTMKAC	1 cut(s)	1272
AccII	CGCG	3 cut(s)	501, 2234, 2236
AclWI	GGATC	5 cut(s)	647, 739, 953, 2313, 2812
AcoI	YGGCCR	4 cut(s)	324, 432, 1282, 2079
AcsI	RAATTY	5 cut(s)	662, 953, 1094, 1576, 2351
AcuI	CTGAAG	6 cut(s)	979, 1499, 1753, 2042, 2510, 2883
AflIII	ACRYGT	1 cut(s)	2361
AgeI	ACCGGT	1 cut(s)	2824
AjnI	CCWGG	2 cut(s)	1029, 2005
AloI	GAACNNNNNNTCC	2 cut(s)	1508, 1540
Alw21I	GWGCWC	2 cut(s)	813, 2646
Alw26I	GTCTC	6 cut(s)	1052, 1500, 1995, 2030, 2352, 2895
Alw44I	GTGCAC	1 cut(s)	2642
AlwI	GGATC	5 cut(s)	647, 739, 953, 2313, 2812
AlwNI	CAGNNNCTG	1 cut(s)	1603
ApaI	GGGCCC	1 cut(s)	296
ApaLI	GTGCAC	1 cut(s)	2642
ApeKI	GCWGC	8 cut(s)	202, 211, 989, 1466, 2064, 2261, 2291, 2306
ApoI	RAATTY	5 cut(s)	662, 953, 1094, 1576, 2351
AsiGI	ACCGGT	1 cut(s)	2824
Asp700I	GAANNNNTTC	1 cut(s)	704
AspLEI	GCGC	4 cut(s)	304, 503, 1080, 2236
AsuC2I	CCSGG	1 cut(s)	2616
AsuHPI	GGTGA	9 cut(s)	98, 704, 1187, 1491, 1681, 1690, 2604, 2776, 2928
AsuII	TTCGAA	1 cut(s)	659
AvaII	GGWCC	4 cut(s)	329, 1034, 1550, 1795
AxyI	CCTNAGG	1 cut(s)	753
BaeGI	GKGCMC	3 cut(s)	296, 473, 2646
BalI	TGGCCA	2 cut(s)	1284, 2081
BanI	GGYRCC	2 cut(s)	403, 2553
BanII	GRGCYC	3 cut(s)	296, 813, 2689
BauI	CACGAG	2 cut(s)	2310, 2843
BbsI	GAAGAC	1 cut(s)	1727
Bbv12I	GWGCWC	2 cut(s)	813, 2646
BbvI	GCAGC	8 cut(s)	214, 223, 976, 1478, 2051, 2273, 2278, 2293
BceAI	ACGGC	4 cut(s)	470, 703, 1454, 1742
BcgI	CGANNNNNNTGC	2 cut(s)	2543, 2577
BciT130I	CCWGG	2 cut(s)	1031, 2007
BciVI	GTATCC	3 cut(s)	20, 1449, 2914
BclI	TGATCA	1 cut(s)	88
BcnI	CCSGG	1 cut(s)	2616
BcoDI	GTCTC	6 cut(s)	1052, 1500, 1995, 2030, 2352, 2895
BfaI	CTAG	4 cut(s)	492, 912, 1899, 2483
BfmI	CTRYAG	1 cut(s)	1269
BfoI	RGCGCY	1 cut(s)	305
BfuAI	ACCTGC	1 cut(s)	415
BfuI	GTATCC	3 cut(s)	20, 1449, 2914
BglI	GCCNNNNNGGC	1 cut(s)	332
BglII	AGATCT	1 cut(s)	2485
Bme1390I	CCNGG	3 cut(s)	1031, 2007, 2616
Bme18I	GGWCC	4 cut(s)	329, 1034, 1550, 1795
BmrFI	CCNGG	3 cut(s)	1031, 2007, 2616
BmrI	ACTGGG	2 cut(s)	1850, 2317
BmsI	GCATC	6 cut(s)	1153, 1255, 1575, 1899, 2026, 2743
BmuI	ACTGGG	2 cut(s)	1850, 2317
BoxI	GACNNNNGTC	1 cut(s)	2364
BpiI	GAAGAC	1 cut(s)	1727
BpmI	CTGGAG	4 cut(s)	797, 2390, 2594, 2877
Bpu10I	CCTNAGC	1 cut(s)	839
Bpu14I	TTCGAA	1 cut(s)	659
BpuEI	CTTGAG	1 cut(s)	2105
BpuMI	CCSGG	1 cut(s)	2616
BsaBI	GATNNNNATC	1 cut(s)	2220
BsaI	GGTCTC	1 cut(s)	1995
BsaJI	CCNNGG	8 cut(s)	260, 973, 1029, 1030, 1295, 2005, 2582, 2689
BsaWI	WCCGGW	4 cut(s)	568, 1197, 1556, 2824
BsaXI	ACNNNNNCTCC	6 cut(s)	46, 76, 1848, 1878, 2873, 2903
Bse118I	RCCGGY	2 cut(s)	331, 2824
Bse1I	ACTGG	9 cut(s)	733, 1610, 1797, 1804, 1845, 2098, 2288, 2323, 2452
Bse21I	CCTNAGG	1 cut(s)	753
Bse3DI	GCAATG	3 cut(s)	381, 1490, 1966
Bse8I	GATNNNNATC	1 cut(s)	2220
BseBI	CCWGG	2 cut(s)	1031, 2007
BseDI	CCNNGG	8 cut(s)	260, 973, 1029, 1030, 1295, 2005, 2582, 2689
BseGI	GGATG	5 cut(s)	1025, 1168, 2224, 2518, 2593
BseJI	GATNNNNATC	1 cut(s)	2220
BseMI	GCAATG	3 cut(s)	381, 1490, 1966
BseMII	CTCAG	5 cut(s)	744, 1071, 1629, 2025, 2760
BseNI	ACTGG	9 cut(s)	733, 1610, 1797, 1804, 1845, 2098, 2288, 2323, 2452
BseRI	GAGGAG	6 cut(s)	144, 153, 2899, 2902, 2911, 2914
BseSI	GKGCMC	3 cut(s)	296, 473, 2646
BseX3I	CGGCCG	1 cut(s)	324
BseXI	GCAGC	8 cut(s)	214, 223, 976, 1478, 2051, 2273, 2278, 2293
BseYI	CCCAGC	1 cut(s)	1752
BsgI	GTGCAG	4 cut(s)	732, 2208, 2292, 2963
Bsh1236I	CGCG	3 cut(s)	501, 2234, 2236
Bsh1285I	CGRYCG	1 cut(s)	327
BshNI	GGYRCC	2 cut(s)	403, 2553
BshTI	ACCGGT	1 cut(s)	2824
BsiEI	CGRYCG	1 cut(s)	327
BsiHKAI	GWGCWC	2 cut(s)	813, 2646
BsiSI	CCGG	9 cut(s)	323, 327, 332, 371, 569, 1198, 1557, 2615, 2825
BsiWI	CGTACG	1 cut(s)	1215
BslFI	GGGAC	5 cut(s)	255, 1340, 1616, 2741, 2898
BsmAI	GTCTC	6 cut(s)	1052, 1500, 1995, 2030, 2352, 2895
BsmFI	GGGAC	5 cut(s)	255, 1340, 1616, 2741, 2898
BsmI	GAATGC	1 cut(s)	1249
Bso31I	GGTCTC	1 cut(s)	1995
Bsp119I	TTCGAA	1 cut(s)	659
Bsp120I	GGGCCC	1 cut(s)	292
Bsp1286I	GDGCHC	5 cut(s)	296, 473, 813, 2646, 2689
Bsp1407I	TGTACA	1 cut(s)	2798
Bsp19I	CCATGG	1 cut(s)	260
BspCNI	CTCAG	5 cut(s)	745, 1070, 1630, 2026, 2759
BspFNI	CGCG	3 cut(s)	501, 2234, 2236
BspMI	ACCTGC	1 cut(s)	415
BspPI	GGATC	5 cut(s)	647, 739, 953, 2313, 2812
BspT104I	TTCGAA	1 cut(s)	659
BspT107I	GGYRCC	2 cut(s)	403, 2553
BspTNI	GGTCTC	1 cut(s)	1995
BsrBI	CCGCTC	1 cut(s)	275
BsrDI	GCAATG	3 cut(s)	381, 1490, 1966
BsrFI	RCCGGY	2 cut(s)	331, 2824
BsrGI	TGTACA	1 cut(s)	2798
BsrI	ACTGG	9 cut(s)	733, 1610, 1797, 1804, 1845, 2098, 2288, 2323, 2452
BssAI	RCCGGY	2 cut(s)	331, 2824
BssECI	CCNNGG	8 cut(s)	260, 973, 1029, 1030, 1295, 2005, 2582, 2689
BssSI	CACGAG	2 cut(s)	2310, 2843
BssT1I	CCWWGG	5 cut(s)	260, 973, 1295, 2582, 2689
Bst2BI	CACGAG	2 cut(s)	2310, 2843
Bst2UI	CCWGG	2 cut(s)	1031, 2007
Bst4CI	ACNGT	8 cut(s)	177, 771, 1189, 1347, 1684, 2278, 2623, 2889
Bst6I	CTCTTC	3 cut(s)	43, 145, 2469
BstAPI	GCANNNNNTGC	2 cut(s)	2114, 2270
BstAUI	TGTACA	1 cut(s)	2798
BstBI	TTCGAA	1 cut(s)	659
BstC8I	GCNNGC	8 cut(s)	170, 246, 306, 801, 2191, 2289, 2602, 2982
BstDEI	CTNAG	8 cut(s)	582, 753, 839, 1057, 1420, 1638, 2034, 2746
BstDSI	CCRYGG	1 cut(s)	260
BstF5I	GGATG	5 cut(s)	1025, 1168, 2224, 2518, 2593
BstFNI	CGCG	3 cut(s)	501, 2234, 2236
BstH2I	RGCGCY	1 cut(s)	305
BstHHI	GCGC	4 cut(s)	304, 503, 1080, 2236
BstMAI	GTCTC	6 cut(s)	1052, 1500, 1995, 2030, 2352, 2895
BstMCI	CGRYCG	1 cut(s)	327
BstNI	CCWGG	2 cut(s)	1031, 2007
BstNSI	RCATGY	2 cut(s)	1084, 2365
BstPAI	GACNNNNGTC	1 cut(s)	2364
BstSCI	CCNGG	3 cut(s)	1029, 2005, 2614
BstSFI	CTRYAG	1 cut(s)	1269
BstSLI	GKGCMC	3 cut(s)	296, 473, 2646
BstUI	CGCG	3 cut(s)	501, 2234, 2236
BstV1I	GCAGC	8 cut(s)	214, 223, 976, 1478, 2051, 2273, 2278, 2293
BstV2I	GAAGAC	1 cut(s)	1727
BstX2I	RGATCY	1 cut(s)	2485
BstYI	RGATCY	1 cut(s)	2485
BstZI	CGGCCG	1 cut(s)	324
Bsu36I	CCTNAGG	1 cut(s)	753
BsuI	GTATCC	3 cut(s)	20, 1449, 2914
BtgI	CCRYGG	1 cut(s)	260
BtgZI	GCGATG	1 cut(s)	1807
BtsCI	GGATG	5 cut(s)	1025, 1168, 2224, 2518, 2593
BtsI	GCAGTG	4 cut(s)	67, 720, 1919, 2066
BtsIMutI	CAGTG	9 cut(s)	67, 715, 720, 726, 1617, 1919, 2066, 2274, 2449
BveI	ACCTGC	1 cut(s)	415
Cac8I	GCNNGC	8 cut(s)	170, 246, 306, 801, 2191, 2289, 2602, 2982
CaiI	CAGNNNCTG	1 cut(s)	1603
CfoI	GCGC	4 cut(s)	304, 503, 1080, 2236
Cfr10I	RCCGGY	2 cut(s)	331, 2824
CpoI	CGGWCCG	1 cut(s)	329
CseI	GACGC	3 cut(s)	924, 1921, 2065
CspAI	ACCGGT	1 cut(s)	2824
CspCI	CAANNNNNGTGG	2 cut(s)	1623, 1658
CspI	CGGWCCG	1 cut(s)	329
DdeI	CTNAG	8 cut(s)	582, 753, 839, 1057, 1420, 1638, 2034, 2746
EaeI	YGGCCR	4 cut(s)	324, 432, 1282, 2079
EagI	CGGCCG	1 cut(s)	324
Eam1104I	CTCTTC	3 cut(s)	43, 145, 2469
EarI	CTCTTC	3 cut(s)	43, 145, 2469
EciI	GGCGGA	1 cut(s)	300
Ecl136II	GAGCTC	1 cut(s)	811
EclXI	CGGCCG	1 cut(s)	324
Eco130I	CCWWGG	5 cut(s)	260, 973, 1295, 2582, 2689
Eco147I	AGGCCT	1 cut(s)	253
Eco24I	GRGCYC	3 cut(s)	296, 813, 2689
Eco31I	GGTCTC	1 cut(s)	1995
Eco32I	GATATC	3 cut(s)	606, 859, 1524
Eco47I	GGWCC	4 cut(s)	329, 1034, 1550, 1795
Eco52I	CGGCCG	1 cut(s)	324
Eco53kI	GAGCTC	1 cut(s)	811
Eco57I	CTGAAG	6 cut(s)	979, 1499, 1753, 2042, 2510, 2883
Eco81I	CCTNAGG	1 cut(s)	753
EcoICRI	GAGCTC	1 cut(s)	811
EcoO109I	RGGNCCY	3 cut(s)	292, 293, 1034
EcoRII	CCWGG	2 cut(s)	1029, 2005
EcoRV	GATATC	3 cut(s)	606, 859, 1524
EcoT14I	CCWWGG	5 cut(s)	260, 973, 1295, 2582, 2689
EcoT22I	ATGCAT	1 cut(s)	1168
EcoT38I	GRGCYC	3 cut(s)	296, 813, 2689
ErhI	CCWWGG	5 cut(s)	260, 973, 1295, 2582, 2689
FalI	AAGNNNNNCTT	2 cut(s)	1293, 1325
FaqI	GGGAC	5 cut(s)	255, 1340, 1616, 2741, 2898
FauI	CCCGC	3 cut(s)	89, 161, 280
FauNDI	CATATG	1 cut(s)	685
FbaI	TGATCA	1 cut(s)	88
FblI	GTMKAC	1 cut(s)	1272
FokI	GGATG	5 cut(s)	1012, 1175, 2211, 2505, 2600
FriOI	GRGCYC	3 cut(s)	296, 813, 2689
FspBI	CTAG	4 cut(s)	492, 912, 1899, 2483
GlaI	GCGC	4 cut(s)	303, 502, 1079, 2235
GsaI	CCCAGC	1 cut(s)	1756
GsuI	CTGGAG	4 cut(s)	797, 2390, 2594, 2877
HaeII	RGCGCY	1 cut(s)	305
HapII	CCGG	9 cut(s)	323, 327, 332, 371, 569, 1198, 1557, 2615, 2825
HgaI	GACGC	3 cut(s)	924, 1921, 2065
HhaI	GCGC	4 cut(s)	304, 503, 1080, 2236
Hin6I	GCGC	4 cut(s)	302, 501, 1078, 2234
HinP1I	GCGC	4 cut(s)	302, 501, 1078, 2234
HincII	GTYRAC	2 cut(s)	1594, 1687
HindII	GTYRAC	2 cut(s)	1594, 1687
HindIII	AAGCTT	3 cut(s)	797, 2135, 2762
HpaII	CCGG	9 cut(s)	323, 327, 332, 371, 569, 1198, 1557, 2615, 2825
HphI	GGTGA	9 cut(s)	98, 704, 1187, 1491, 1681, 1690, 2604, 2776, 2928
HpyCH4III	ACNGT	8 cut(s)	177, 771, 1189, 1347, 1684, 2278, 2623, 2889
HpyCH4IV	ACGT	2 cut(s)	1214, 1218
HpyF3I	CTNAG	8 cut(s)	582, 753, 839, 1057, 1420, 1638, 2034, 2746
HpySE526I	ACGT	2 cut(s)	1214, 1218
HspAI	GCGC	4 cut(s)	302, 501, 1078, 2234
Ksp22I	TGATCA	1 cut(s)	88
LmnI	GCTCC	8 cut(s)	280, 313, 358, 808, 816, 2175, 2371, 2684
Lsp1109I	GCAGC	8 cut(s)	214, 223, 976, 1478, 2051, 2273, 2278, 2293
LweI	GCATC	6 cut(s)	1153, 1255, 1575, 1899, 2026, 2743
MaeI	CTAG	4 cut(s)	492, 912, 1899, 2483
MaeII	ACGT	2 cut(s)	1214, 1218
MaeIII	GTNAC	8 cut(s)	1087, 1183, 1189, 1612, 1678, 1974, 2009, 2053
MbiI	CCGCTC	1 cut(s)	275
MfeI	CAATTG	1 cut(s)	2256
MflI	RGATCY	1 cut(s)	2485
MhlI	GDGCHC	5 cut(s)	296, 473, 813, 2646, 2689
MlsI	TGGCCA	2 cut(s)	1284, 2081
MluNI	TGGCCA	2 cut(s)	1284, 2081
MlyI	GAGTC	6 cut(s)	247, 1186, 1297, 1717, 2032, 2448
MmeI	TCCRAC	3 cut(s)	834, 1551, 2546
Mox20I	TGGCCA	2 cut(s)	1284, 2081
Mph1103I	ATGCAT	1 cut(s)	1168
MroXI	GAANNNNTTC	1 cut(s)	704
MscI	TGGCCA	2 cut(s)	1284, 2081
MseI	TTAA	6 cut(s)	513, 564, 674, 738, 1127, 1674
MslI	CAYNNNNRTG	3 cut(s)	552, 1883, 2715
Msp20I	TGGCCA	2 cut(s)	1284, 2081
MspA1I	CMGCKG	3 cut(s)	850, 2941, 2948
MspI	CCGG	9 cut(s)	323, 327, 332, 371, 569, 1198, 1557, 2615, 2825
MspR9I	CCNGG	3 cut(s)	1031, 2007, 2616
MunI	CAATTG	1 cut(s)	2256
Mva1269I	GAATGC	1 cut(s)	1249
MvaI	CCWGG	2 cut(s)	1031, 2007
MvnI	CGCG	3 cut(s)	501, 2234, 2236
NciI	CCSGG	1 cut(s)	2616
NcoI	CCATGG	1 cut(s)	260
NdeI	CATATG	1 cut(s)	685
NmuCI	GTSAC	4 cut(s)	1189, 1612, 1678, 2053
NsiI	ATGCAT	1 cut(s)	1168
NspI	RCATGY	2 cut(s)	1084, 2365
NspV	TTCGAA	1 cut(s)	659
PaqCI	CACCTGC	1 cut(s)	415
PasI	CCCWGGG	1 cut(s)	1030
PceI	AGGCCT	1 cut(s)	253
PciI	ACATGT	1 cut(s)	2361
PctI	GAATGC	1 cut(s)	1249
PdmI	GAANNNNTTC	1 cut(s)	704
Pfl23II	CGTACG	1 cut(s)	1215
PflMI	CCANNNNNTGG	3 cut(s)	288, 2335, 2588
PinAI	ACCGGT	1 cut(s)	2824
PleI	GAGTC	6 cut(s)	246, 1186, 1296, 1717, 2032, 2447
PpsI	GAGTC	6 cut(s)	246, 1186, 1296, 1717, 2032, 2447
PpuMI	RGGWCCY	1 cut(s)	1034
PscI	ACATGT	1 cut(s)	2361
PshAI	GACNNNNGTC	1 cut(s)	2364
PsiI	TTATAA	1 cut(s)	542
Psp124BI	GAGCTC	1 cut(s)	813
Psp5II	RGGWCCY	1 cut(s)	1034
Psp6I	CCWGG	2 cut(s)	1029, 2005
PspFI	CCCAGC	1 cut(s)	1752
PspGI	CCWGG	2 cut(s)	1029, 2005
PspLI	CGTACG	1 cut(s)	1215
PspOMI	GGGCCC	1 cut(s)	292
PspPPI	RGGWCCY	1 cut(s)	1034
PstNI	CAGNNNCTG	1 cut(s)	1603
PsuI	RGATCY	1 cut(s)	2485
PvuII	CAGCTG	1 cut(s)	850
RseI	CAYNNNNRTG	3 cut(s)	552, 1883, 2715
Rsr2I	CGGWCCG	1 cut(s)	329
RsrII	CGGWCCG	1 cut(s)	329
SacI	GAGCTC	1 cut(s)	813
SaqAI	TTAA	6 cut(s)	513, 564, 674, 738, 1127, 1674
SchI	GAGTC	6 cut(s)	247, 1186, 1297, 1717, 2032, 2448
ScrFI	CCNGG	3 cut(s)	1031, 2007, 2616
SduI	GDGCHC	5 cut(s)	296, 473, 813, 2646, 2689
SfaNI	GCATC	6 cut(s)	1153, 1255, 1575, 1899, 2026, 2743
SfcI	CTRYAG	1 cut(s)	1269
SfiI	GGCCNNNNNGGCC	1 cut(s)	332
SfuI	TTCGAA	1 cut(s)	659
SgrAI	CRCCGGYG	1 cut(s)	2824
SinI	GGWCC	4 cut(s)	329, 1034, 1550, 1795
SmiMI	CAYNNNNRTG	3 cut(s)	552, 1883, 2715
SmlI	CTYRAG	1 cut(s)	2120
SmoI	CTYRAG	1 cut(s)	2120
SseBI	AGGCCT	1 cut(s)	253
SspMI	CTAG	4 cut(s)	492, 912, 1899, 2483
SstI	GAGCTC	1 cut(s)	813
StuI	AGGCCT	1 cut(s)	253
StyD4I	CCNGG	3 cut(s)	1029, 2005, 2614
StyI	CCWWGG	5 cut(s)	260, 973, 1295, 2582, 2689
TaaI	ACNGT	8 cut(s)	177, 771, 1189, 1347, 1684, 2278, 2623, 2889
TaiI	ACGT	2 cut(s)	1217, 1221
TaqI	TCGA	4 cut(s)	659, 1050, 2317, 2927
TatI	WGTACW	3 cut(s)	1347, 2798, 2829
TauI	GCSGC	5 cut(s)	15, 434, 2234, 2554, 2951
Tru1I	TTAA	6 cut(s)	513, 564, 674, 738, 1127, 1674
Tru9I	TTAA	6 cut(s)	513, 564, 674, 738, 1127, 1674
TscAI	CASTG	9 cut(s)	67, 715, 720, 733, 1617, 1926, 2066, 2281, 2449
TseFI	GTSAC	4 cut(s)	1189, 1612, 1678, 2053
TseI	GCWGC	8 cut(s)	202, 211, 989, 1466, 2064, 2261, 2291, 2306
Tsp45I	GTSAC	4 cut(s)	1189, 1612, 1678, 2053
TspDTI	ATGAA	8 cut(s)	402, 911, 1061, 1798, 1887, 1953, 2363, 2976
TspGWI	ACGGA	3 cut(s)	2420, 2697, 2894
TspRI	CASTG	9 cut(s)	67, 715, 720, 733, 1617, 1926, 2066, 2281, 2449
Van91I	CCANNNNNTGG	3 cut(s)	288, 2335, 2588
VneI	GTGCAC	1 cut(s)	2642
VpaK11BI	GGWCC	4 cut(s)	329, 1034, 1550, 1795
XapI	RAATTY	5 cut(s)	662, 953, 1094, 1576, 2351
XceI	RCATGY	2 cut(s)	1084, 2365
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1749
XmiI	GTMKAC	1 cut(s)	1272
XmnI	GAANNNNTTC	1 cut(s)	704
XspI	CTAG	4 cut(s)	492, 912, 1899, 2483
Zsp2I	ATGCAT	1 cut(s)	1168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.