Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007187

Physical Location & Seq

Genomic Coordinates Forward (+)

33296860 .. 33300848

Gene Structure 3989 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1236400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2685 bp

ATGTGTTACACTTATACTTTTGTTCCTCGATTGCGATCTCTGGAAATTTCGAAACAGACGCCTCCTTCATCTTCTTCGGGCAATGCAGACAAGAATCTCTCAGCAAAACGGCCAGACAACGGAGGCACAAAAGCTATCCAAACTGTTATGCTTCGTTCTAATCATTTTAATATCTCGTTCAATCCTGAGAGTATCATAATGCACTACGGTGTTGATGTCAAATCAGAGACCCCTGCTCCTAATCATATGAAAATTTCCAAGTCTGAGCTCTCTGCAATCAGGAAGAAGCTATCCTCTGATAAACCTACGGACTTTCCATTGTCAAGTACTGCATACGATGGTGAAAAGAACATCTTCAGTGCAGTGCGGTTGCCCACTGGATCATTTAAGGTAGAGGTTCCTGATGAAGAAGACACGCGGTTTACTACATACATATTTACTATAAAGTTAGTAAACGAGCTCAAGCTTTGCAAGTTGAAGGAGTACATGAGTGGCCACCTATTGTCTATCCCTCGTGATATATTACACGGTATGGATTTGGTGATGAAAGAGAATCCAAGTAGGCGCTTGATTGCTGTTGGGCGAAACTATTACCCTGTGGATACTAGTCTAAACGATGACCTTGGACATGGTATTTCTGCATCTAGAGGGTTTCAAGATTCTGTAAGGCTCACTTCCCAAGGTCCTTCCCTGTGTCTTGACTACTCAGTCCTGGCATTTTATAAGCGCTTGCCAGTTATAGATTTTCTCCAAGAGCAGATTACGGGTTTTACTTTAAATAATTTCGAGAGGTTTAGGAGAGAGGTTGAGCGTGTGTTGAGAGGACTGAAAGTAACTGTGATGCACCGTAAAACCAAGCAAAAGTACATCATCAAGGGGCTGACTGATAAGAATGCAGGAGATATTACATTTTATGATGTAGACATAGATGGCCAGTTTCCGCCAAGGAAAGTTAGACTTGTCGATTATTTCAGGGAAAGATACAAGGGAATCCAGTATAAAGACATTCCTTGCTTGGATTTGGGGAAAAATGGTAGAAGGAACGATACACCTATGGAGTTCTGTGTGTTAGCTGAGGGGCAAAAGTATTCAAAGGAGCGCTTGGACAGAGAAGCAGCCATGATGTTGAGGAGTAGGTCATTGGCTCAACCGCGTGATAGGCAGAGGAAGATATGCGACATTATACAATCTGATGATGGACCCTGTGGTGGTGGCATAATTGAAAATTTTGGAATTGAAATTAACAAGAATATGACACCAGTAACAGGGCGTGTCATTGGCCCACCAGAATTGAAGTTCGGTGCTTCTGATGGCAAGGTGAATAAGGTAACCGTTGACAGAGAGAAATGCAACTGGAATCTGATTGGAAAGTCTGTAATGGAAGGAAAACCAATCAATCGCTGGGCTTTACTTGACTTCACCTGTCATGATCGATTTGTACTGAACCACGGCTTCATCCCAAAGCTCATCAATCGGTGCAATGAGCTGGGAATCAGAATGGAAGAGCCTCTTTCATACCAAGGCACTACAATGAGACCCTTCTCTAGTGTTCAGATGCTTCGCCAGGTACTTGAAAGTGTTAATGAAGAGGTACACAAGAAAGGAAAAGGCTACTTGCAATTAATTATTTGTGTAATGCCTAGAAGAGATCCTGGTTACAAGTATCTGAAGTGGATCTCCGAGACTCAAATTGGTATAGTGACACAGTGCTGCTTGTCAAATATGGCCAACAAAGCAAATGAAAAGTATCTTGGAAACCTTGCACTTAAGATTAATGCAAAGCTTGGAGGTAGTAATGTAGAGCTAATTGATCGGCTCCCCCTCTTTGGGGGTGCAGGCCATGTTATGTTTATGGGGGCCGATGTCAATCATCCTGCTGCGCAAAACAAAAAAAGTCCATCAATTGCGGCTGTTGTTGCCACTGTAAACTGGCCTGCTTTGAATCGTTATGCTGCACGAGTTCGACCCCAGCAACACCGTAGCGAAAGGATATTGAGTTTTGGAGACATGTGCCTGGAGCTTGTTGAAACTTATGAGCGATTGAATAAAGTCAAGCCAGACAGAATTGTTGTCTTCCGTGATGGAGTCAGTGAGGGCCAGTTTGATATGGTTCTAAATGAAGAGTTACTAGATATGAAGAGGGCTTTCGGATGTAAAAAATACTATCCTACCATAACACTTGTTGTTGCCCAAAAGCGGCACCATACTCGTTTGTTTCTAGAGAATGTGAAGGATGGGAGTCCAACATGCAATGTGTTACCGGGAACTGTTGTGGATACAACAATCGTGCACCCGTTTGAGTTTGACTTTTATCTTTGCAGTCATTATGGATCCCTCGGAACAAGCAAACCCACACACTACTATGTTCTTTGGGATGAGCATAGGTTTACCTCTGACCAATTGCAGAAGCTCATTTATGACTTATGCTTCACCTTAGCAAGGTGTACTAAACCTGTAGCTTTGGTACCACCGGTATACTATGCTGACCTTGTTGCATATAGAGGGCGGTTATACCATGAGAGTATCGAGCGACAGTCTCGAGATTCAGTATCTTCCTCCTCCTACTATTCCTCATCATCATCAGCAGCAGCAGCATTGGCATCGTCACCACACTCAGTGGCTTCTCTAGATGATAAGTTTTATAAATTGCATGCTGACCTGGAAAACATAATGTTTTTCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0016032 GO:0019048 GO:0035197 GO:0035821 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0061980 GO:0097159 GO:0098542 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

895

Amino Acids

101.42

Weight (kDa)

9.11

Isoelectric Point (pI)

42.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	48 - 182	1.4e-20	N-terminal domain of argonaute
ArgoL1	PF08699	193 - 242	1.2e-11	Argonaute linker 1 domain
PAZ	PF02170	261 - 370	3.3e-20	PAZ domain
ArgoL2	PF16488	405 - 433	5.6e-07	Argonaute linker 2 domain
Piwi	PF02171	541 - 836	1.8e-88	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31280 AT1G31290 AT1G31290 AT1G31290
fragaria_vesca	FvH4_3g11980
malus_domestica	MD05G1256100.v1.1 MD10G1236300.v1.1 MD10G1236400.v1.1
prunus_persica	Prupe.4G108000_v2.0.a1 Prupe.4G108100_v2.0.a1
pyrus_communis	pycom05g23500 pycom05g23510 pycom05g23520 pycom10g19770 pycom10g19780
rosa_chinensis	RchiOBHm_Chr5g0019581
rosa_laevigata	RLG00000032457
rosa_roxburghii	Rroxscaffold_1G00058500
rosa_rugosa	Rorug05G0052100 Rorug05G0052200
rosa_samantha	Rh5AG142700 Rh5BG141200 Rh5CG153300 Rh5DG141900
rosa_wichuraiana	Rw5G012630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	723, 2643
AasI	GACNNNNNNGTC	1 cut(s)	707
Acc16I	TGCGCA	1 cut(s)	1881
Acc65I	GGTACC	1 cut(s)	2464
AccB1I	GGYRCC	2 cut(s)	2199, 2464
AccI	GTMKAC	2 cut(s)	921, 2475
AccII	CGCG	2 cut(s)	418, 1153
AciI	CCGC	7 cut(s)	367, 418, 941, 1151, 1907, 2197, 2506
AclWI	GGATC	5 cut(s)	388, 1643, 1682, 2325, 2338
AcoI	YGGCCR	4 cut(s)	110, 493, 931, 1725
AcsI	RAATTY	3 cut(s)	45, 252, 1225
AcuI	CTGAAG	2 cut(s)	340, 1688
AcyI	GRCGYC	1 cut(s)	59
AdeI	CACNNNGTG	1 cut(s)	2617
AfaI	GTAC	8 cut(s)	328, 485, 866, 1440, 1569, 1593, 2446, 2466
AfeI	AGCGCT	2 cut(s)	728, 1100
AfiI	CCNNNNNNNGG	8 cut(s)	119, 1208, 1265, 1826, 1827, 1828, 2196, 2439
AflII	CTTAAG	1 cut(s)	1766
AflIII	ACRYGT	1 cut(s)	2007
AgeI	ACCGGT	1 cut(s)	2470
AhlI	ACTAGT	1 cut(s)	605
AjnI	CCWGG	5 cut(s)	711, 1563, 1651, 2013, 2658
AjuI	GAANNNNNNNTTGG	2 cut(s)	1734, 1766
AleI	CACNNNNGTG	1 cut(s)	207
Alw21I	GWGCWC	3 cut(s)	270, 462, 2292
Alw26I	GTCTC	5 cut(s)	221, 1528, 1676, 1998, 2541
Alw44I	GTGCAC	1 cut(s)	2288
AlwI	GGATC	5 cut(s)	388, 1643, 1682, 2325, 2338
Ama87I	CYCGRG	1 cut(s)	2538
Aor51HI	AGCGCT	2 cut(s)	728, 1100
AoxI	GGCC	9 cut(s)	110, 493, 931, 1279, 1725, 1837, 1857, 1931, 2095
ApaLI	GTGCAC	1 cut(s)	2288
ApeKI	GCWGC	7 cut(s)	1115, 1710, 1877, 1952, 2585, 2588, 2591
ApoI	RAATTY	3 cut(s)	45, 252, 1225
AseI	ATTAAT	2 cut(s)	1622, 1773
AsiGI	ACCGGT	1 cut(s)	2470
Asp700I	GAANNNNTTC	1 cut(s)	353
Asp718I	GGTACC	1 cut(s)	2464
AspLEI	GCGC	4 cut(s)	567, 729, 1101, 1882
AspS9I	GGNCC	5 cut(s)	683, 1199, 1280, 1857, 2095
AsuC2I	CCSGG	1 cut(s)	2262
AsuHPI	GGTGA	6 cut(s)	353, 553, 1330, 1411, 2422, 2598
AsuII	TTCGAA	1 cut(s)	50
AvaI	CYCGRG	1 cut(s)	2538
AvaII	GGWCC	2 cut(s)	683, 1199
BaeGI	GKGCMC	1 cut(s)	2292
BalI	TGGCCA	3 cut(s)	495, 933, 1727
BamHI	GGATCC	1 cut(s)	2330
BanI	GGYRCC	2 cut(s)	2199, 2464
BanII	GRGCYC	2 cut(s)	270, 462
BarI	GAAGNNNNNNTAC	2 cut(s)	1030, 1062
BauI	CACGAG	2 cut(s)	513, 1956
BbsI	GAAGAC	2 cut(s)	417, 2065
Bbv12I	GWGCWC	3 cut(s)	270, 462, 2292
BbvCI	CCTCAGC	1 cut(s)	1074
BbvI	GCAGC	7 cut(s)	1127, 1697, 1864, 1939, 2597, 2600, 2603
BccI	CCATC	7 cut(s)	332, 923, 1190, 1304, 1906, 2075, 2228
BceAI	ACGGC	2 cut(s)	125, 1465
BcgI	CGANNNNNNTGC	4 cut(s)	2189, 2223, 2583, 2617
BciT130I	CCWGG	5 cut(s)	713, 1565, 1653, 2015, 2660
BciVI	GTATCC	2 cut(s)	595, 2269
BcnI	CCSGG	1 cut(s)	2262
BcoDI	GTCTC	5 cut(s)	221, 1528, 1676, 1998, 2541
BcuI	ACTAGT	1 cut(s)	605
BfaI	CTAG	7 cut(s)	606, 645, 1545, 1641, 2129, 2219, 2627
BfmI	CTRYAG	1 cut(s)	2454
BfoI	RGCGCY	3 cut(s)	568, 730, 1102
BfrI	CTTAAG	1 cut(s)	1766
BfuI	GTATCC	2 cut(s)	595, 2269
BisI	GCNGC	9 cut(s)	1116, 1711, 1878, 1908, 1953, 2198, 2586, 2589, 2592
BlsI	GCNGC	9 cut(s)	1117, 1712, 1879, 1909, 1954, 2199, 2587, 2590, 2593
BmcAI	AGTACT	1 cut(s)	328
Bme1390I	CCNGG	6 cut(s)	713, 1565, 1653, 2015, 2262, 2660
Bme18I	GGWCC	2 cut(s)	683, 1199
BmeT110I	CYCGRG	1 cut(s)	2538
BmgT120I	GGNCC	5 cut(s)	683, 1199, 1280, 1857, 2095
BmiI	GGNNCC	7 cut(s)	399, 1201, 1817, 1858, 2201, 2332, 2466
BmrFI	CCNGG	6 cut(s)	713, 1565, 1653, 2015, 2262, 2660
BmsI	GCATC	4 cut(s)	650, 831, 1545, 2609
BpiI	GAAGAC	2 cut(s)	417, 2065
BpmI	CTGGAG	1 cut(s)	2036
Bpu10I	CCTNAGC	2 cut(s)	1074, 2434
Bpu14I	TTCGAA	1 cut(s)	50
BpuEI	CTTGAG	1 cut(s)	446
BpuMI	CCSGG	1 cut(s)	2262
Bsa29I	ATCGAT	1 cut(s)	1432
BsaBI	GATNNNNATC	2 cut(s)	34, 1866
BsaHI	GRCGYC	1 cut(s)	59
BsaI	GGTCTC	2 cut(s)	221, 1528
BsaJI	CCNNGG	6 cut(s)	622, 679, 944, 1447, 1519, 2335
BsaWI	WCCGGW	1 cut(s)	2470
BsaXI	ACNNNNNCTCC	2 cut(s)	220, 250
Bsc4I	CCNNNNNNNGG	8 cut(s)	119, 1208, 1265, 1826, 1827, 1828, 2196, 2439
Bse118I	RCCGGY	1 cut(s)	2470
Bse1I	ACTGG	8 cut(s)	382, 734, 934, 994, 1259, 1358, 1934, 2098
Bse3DI	GCAATG	3 cut(s)	88, 1486, 2257
Bse8I	GATNNNNATC	2 cut(s)	34, 1866
BseBI	CCWGG	5 cut(s)	713, 1565, 1653, 2015, 2660
BseCI	ATCGAT	1 cut(s)	1432
BseDI	CCNNGG	6 cut(s)	622, 679, 944, 1447, 1519, 2335
BseGI	GGATG	5 cut(s)	1455, 1870, 2156, 2239, 2380
BseJI	GATNNNNATC	2 cut(s)	34, 1866
BseLI	CCNNNNNNNGG	8 cut(s)	119, 1208, 1265, 1826, 1827, 1828, 2196, 2439
BseMI	GCAATG	3 cut(s)	88, 1486, 2257
BseMII	CTCAG	6 cut(s)	114, 177, 255, 720, 1065, 2628
BseNI	ACTGG	8 cut(s)	382, 734, 934, 994, 1259, 1358, 1934, 2098
BseRI	GAGGAG	2 cut(s)	1144, 2548
BseSI	GKGCMC	1 cut(s)	2292
BseXI	GCAGC	7 cut(s)	1127, 1697, 1864, 1939, 2597, 2600, 2603
BseYI	CCCAGC	3 cut(s)	1401, 1486, 1968
BsgI	GTGCAG	3 cut(s)	381, 1854, 1938
Bsh1236I	CGCG	2 cut(s)	418, 1153
BshFI	GGCC	9 cut(s)	112, 495, 933, 1281, 1727, 1839, 1859, 1933, 2097
BshNI	GGYRCC	2 cut(s)	2199, 2464
BshTI	ACCGGT	1 cut(s)	2470
BshVI	ATCGAT	1 cut(s)	1432
BsiHKAI	GWGCWC	3 cut(s)	270, 462, 2292
BsiHKCI	CYCGRG	1 cut(s)	2538
BsiSI	CCGG	2 cut(s)	2261, 2471
BslI	CCNNNNNNNGG	8 cut(s)	119, 1208, 1265, 1826, 1827, 1828, 2196, 2439
BsmAI	GTCTC	5 cut(s)	221, 1528, 1676, 1998, 2541
BsmI	GAATGC	1 cut(s)	898
BsnI	GGCC	9 cut(s)	112, 495, 933, 1281, 1727, 1839, 1859, 1933, 2097
Bso31I	GGTCTC	2 cut(s)	221, 1528
BsoBI	CYCGRG	1 cut(s)	2538
Bsp119I	TTCGAA	1 cut(s)	50
Bsp1286I	GDGCHC	3 cut(s)	270, 462, 2292
Bsp143I	GATC	7 cut(s)	35, 380, 1429, 1648, 1674, 1810, 2330
BspACI	CCGC	7 cut(s)	367, 418, 941, 1151, 1907, 2197, 2506
BspANI	GGCC	9 cut(s)	112, 495, 933, 1281, 1727, 1839, 1859, 1933, 2097
BspCNI	CTCAG	6 cut(s)	113, 178, 256, 719, 1066, 2627
BspDI	ATCGAT	1 cut(s)	1432
BspFNI	CGCG	2 cut(s)	418, 1153
BspHI	TCATGA	1 cut(s)	1426
BspLI	GGNNCC	7 cut(s)	399, 1201, 1817, 1858, 2201, 2332, 2466
BspPI	GGATC	5 cut(s)	388, 1643, 1682, 2325, 2338
BspQI	GCTCTTC	1 cut(s)	1497
BspT104I	TTCGAA	1 cut(s)	50
BspT107I	GGYRCC	2 cut(s)	2199, 2464
BspTI	CTTAAG	1 cut(s)	1766
BspTNI	GGTCTC	2 cut(s)	221, 1528
BsrDI	GCAATG	3 cut(s)	88, 1486, 2257
BsrFI	RCCGGY	1 cut(s)	2470
BsrI	ACTGG	8 cut(s)	382, 734, 934, 994, 1259, 1358, 1934, 2098
BssAI	RCCGGY	1 cut(s)	2470
BssECI	CCNNGG	6 cut(s)	622, 679, 944, 1447, 1519, 2335
BssMI	GATC	7 cut(s)	35, 380, 1429, 1648, 1674, 1810, 2330
BssNAI	GTATAC	1 cut(s)	2476
BssNI	GRCGYC	1 cut(s)	59
BssSI	CACGAG	2 cut(s)	513, 1956
BssT1I	CCWWGG	4 cut(s)	622, 679, 944, 1519
Bst1107I	GTATAC	1 cut(s)	2476
Bst2BI	CACGAG	2 cut(s)	513, 1956
Bst2UI	CCWGG	5 cut(s)	713, 1565, 1653, 2015, 2660
Bst6I	CTCTTC	5 cut(s)	1497, 1581, 1639, 2115, 2132
BstACI	GRCGYC	1 cut(s)	59
BstAFI	CTTAAG	1 cut(s)	1766
BstBI	TTCGAA	1 cut(s)	50
BstC8I	GCNNGC	4 cut(s)	731, 1837, 1935, 2652
BstDEI	CTNAG	7 cut(s)	100, 186, 264, 706, 1074, 2434, 2614
BstDSI	CCRYGG	1 cut(s)	1447
BstEII	GGTNACC	1 cut(s)	1327
BstENI	CCTNNNNNAGG	1 cut(s)	2437
BstF5I	GGATG	5 cut(s)	1455, 1870, 2156, 2239, 2380
BstFNI	CGCG	2 cut(s)	418, 1153
BstH2I	RGCGCY	3 cut(s)	568, 730, 1102
BstHHI	GCGC	4 cut(s)	567, 729, 1101, 1882
BstKTI	GATC	7 cut(s)	38, 383, 1432, 1651, 1677, 1813, 2333
BstMAI	GTCTC	5 cut(s)	221, 1528, 1676, 1998, 2541
BstMBI	GATC	7 cut(s)	35, 380, 1429, 1648, 1674, 1810, 2330
BstMWI	GCNNNNNNNGC	5 cut(s)	1159, 1733, 1916, 2591, 2597
BstNI	CCWGG	5 cut(s)	713, 1565, 1653, 2015, 2660
BstNSI	RCATGY	3 cut(s)	2011, 2250, 2654
BstPI	GGTNACC	1 cut(s)	1327
BstSCI	CCNGG	6 cut(s)	711, 1563, 1651, 2013, 2260, 2658
BstSFI	CTRYAG	1 cut(s)	2454
BstSLI	GKGCMC	1 cut(s)	2292
BstUI	CGCG	2 cut(s)	418, 1153
BstV1I	GCAGC	7 cut(s)	1127, 1697, 1864, 1939, 2597, 2600, 2603
BstV2I	GAAGAC	2 cut(s)	417, 2065
BstX2I	RGATCY	3 cut(s)	1648, 1674, 2330
BstYI	RGATCY	3 cut(s)	1648, 1674, 2330
BstZ17I	GTATAC	1 cut(s)	2476
Bsu15I	ATCGAT	1 cut(s)	1432
BsuI	GTATCC	2 cut(s)	595, 2269
BsuRI	GGCC	9 cut(s)	112, 495, 933, 1281, 1727, 1839, 1859, 1933, 2097
BsuTUI	ATCGAT	1 cut(s)	1432
BtgI	CCRYGG	1 cut(s)	1447
BtsCI	GGATG	5 cut(s)	1455, 1870, 2156, 2239, 2380
BtsI	GCAGTG	1 cut(s)	369
BtsIMutI	CAGTG	7 cut(s)	364, 369, 375, 1712, 1920, 2095, 2622
Cac8I	GCNNGC	4 cut(s)	731, 1837, 1935, 2652
CciI	TCATGA	1 cut(s)	1426
CfoI	GCGC	4 cut(s)	567, 729, 1101, 1882
Cfr10I	RCCGGY	1 cut(s)	2470
Cfr13I	GGNCC	5 cut(s)	683, 1199, 1280, 1857, 2095
ClaI	ATCGAT	1 cut(s)	1432
CseI	GACGC	1 cut(s)	67
Csp6I	GTAC	8 cut(s)	327, 484, 865, 1439, 1568, 1592, 2445, 2465
CspAI	ACCGGT	1 cut(s)	2470
CspCI	CAANNNNNGTGG	2 cut(s)	1272, 1307
CviAII	CATG	9 cut(s)	487, 629, 1120, 1427, 1841, 2008, 2247, 2516, 2651
CviQI	GTAC	8 cut(s)	327, 484, 865, 1439, 1568, 1592, 2445, 2465
DdeI	CTNAG	7 cut(s)	100, 186, 264, 706, 1074, 2434, 2614
DpnI	GATC	7 cut(s)	37, 382, 1431, 1650, 1676, 1812, 2332
DpnII	GATC	7 cut(s)	35, 380, 1429, 1648, 1674, 1810, 2330
DraI	TTTAAA	1 cut(s)	777
DraIII	CACNNNGTG	1 cut(s)	2617
DrdI	GACNNNNNNGTC	1 cut(s)	707
DseDI	GACNNNNNNGTC	1 cut(s)	707
EaeI	YGGCCR	4 cut(s)	110, 493, 931, 1725
Eam1104I	CTCTTC	5 cut(s)	1497, 1581, 1639, 2115, 2132
EarI	CTCTTC	5 cut(s)	1497, 1581, 1639, 2115, 2132
EciI	GGCGGA	1 cut(s)	930
Ecl136II	GAGCTC	2 cut(s)	268, 460
Eco130I	CCWWGG	4 cut(s)	622, 679, 944, 1519
Eco24I	GRGCYC	2 cut(s)	270, 462
Eco31I	GGTCTC	2 cut(s)	221, 1528
Eco47I	GGWCC	2 cut(s)	683, 1199
Eco47III	AGCGCT	2 cut(s)	728, 1100
Eco53kI	GAGCTC	2 cut(s)	268, 460
Eco57I	CTGAAG	2 cut(s)	340, 1688
Eco88I	CYCGRG	1 cut(s)	2538
Eco91I	GGTNACC	1 cut(s)	1327
EcoICRI	GAGCTC	2 cut(s)	268, 460
EcoNI	CCTNNNNNAGG	1 cut(s)	2437
EcoO109I	RGGNCCY	1 cut(s)	683
EcoO65I	GGTNACC	1 cut(s)	1327
EcoRII	CCWGG	5 cut(s)	711, 1563, 1651, 2013, 2658
EcoT14I	CCWWGG	4 cut(s)	622, 679, 944, 1519
EcoT38I	GRGCYC	2 cut(s)	270, 462
ErhI	CCWWGG	4 cut(s)	622, 679, 944, 1519
FaeI	CATG	9 cut(s)	490, 632, 1123, 1430, 1844, 2011, 2250, 2519, 2654
FatI	CATG	9 cut(s)	486, 628, 1119, 1426, 1840, 2007, 2246, 2515, 2650
FauNDI	CATATG	2 cut(s)	246, 2681
FblI	GTMKAC	2 cut(s)	921, 2475
Fnu4HI	GCNGC	9 cut(s)	1116, 1711, 1878, 1908, 1953, 2198, 2586, 2589, 2592
FokI	GGATG	5 cut(s)	1442, 1857, 2163, 2246, 2387
FriOI	GRGCYC	2 cut(s)	270, 462
Fsp4HI	GCNGC	9 cut(s)	1116, 1711, 1878, 1908, 1953, 2198, 2586, 2589, 2592
FspBI	CTAG	7 cut(s)	606, 645, 1545, 1641, 2129, 2219, 2627
FspI	TGCGCA	1 cut(s)	1881
GlaI	GCGC	4 cut(s)	566, 728, 1100, 1881
GluI	GCNGC	9 cut(s)	1116, 1711, 1878, 1908, 1953, 2198, 2586, 2589, 2592
GsaI	CCCAGC	3 cut(s)	1405, 1490, 1972
GsuI	CTGGAG	1 cut(s)	2036
HaeII	RGCGCY	3 cut(s)	568, 730, 1102
HaeIII	GGCC	9 cut(s)	112, 495, 933, 1281, 1727, 1839, 1859, 1933, 2097
HapII	CCGG	2 cut(s)	2261, 2471
HgaI	GACGC	1 cut(s)	67
HhaI	GCGC	4 cut(s)	567, 729, 1101, 1882
Hin1I	GRCGYC	1 cut(s)	59
Hin1II	CATG	9 cut(s)	490, 632, 1123, 1430, 1844, 2011, 2250, 2519, 2654
Hin6I	GCGC	4 cut(s)	565, 727, 1099, 1880
HinP1I	GCGC	4 cut(s)	565, 727, 1099, 1880
HincII	GTYRAC	1 cut(s)	1336
HindII	GTYRAC	1 cut(s)	1336
HindIII	AAGCTT	2 cut(s)	464, 1781
HpaII	CCGG	2 cut(s)	2261, 2471
HphI	GGTGA	6 cut(s)	353, 553, 1330, 1411, 2422, 2598
HpyAV	CCTTC	7 cut(s)	75, 472, 696, 1032, 1376, 1549, 2224
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1159, 1733, 1916, 2591, 2597
HpyF3I	CTNAG	7 cut(s)	100, 186, 264, 706, 1074, 2434, 2614
Hsp92I	GRCGYC	1 cut(s)	59
Hsp92II	CATG	9 cut(s)	490, 632, 1123, 1430, 1844, 2011, 2250, 2519, 2654
HspAI	GCGC	4 cut(s)	565, 727, 1099, 1880
KpnI	GGTACC	1 cut(s)	2468
Kzo9I	GATC	7 cut(s)	35, 380, 1429, 1648, 1674, 1810, 2330
LguI	GCTCTTC	1 cut(s)	1497
LmnI	GCTCC	4 cut(s)	241, 1096, 1821, 2017
Lsp1109I	GCAGC	7 cut(s)	1127, 1697, 1864, 1939, 2597, 2600, 2603
LweI	GCATC	4 cut(s)	650, 831, 1545, 2609
MaeI	CTAG	7 cut(s)	606, 645, 1545, 1641, 2129, 2219, 2627
MaeIII	GTNAC	9 cut(s)	5, 832, 1261, 1327, 1655, 1699, 2124, 2256, 2604
MalI	GATC	7 cut(s)	37, 382, 1431, 1650, 1676, 1812, 2332
MboI	GATC	7 cut(s)	35, 380, 1429, 1648, 1674, 1810, 2330
MfeI	CAATTG	2 cut(s)	1902, 2399
MflI	RGATCY	3 cut(s)	1648, 1674, 2330
MhlI	GDGCHC	3 cut(s)	270, 462, 2292
MlsI	TGGCCA	3 cut(s)	495, 933, 1727
MluNI	TGGCCA	3 cut(s)	495, 933, 1727
MlyI	GAGTC	3 cut(s)	1678, 2094, 2248
MmeI	TCCRAC	1 cut(s)	2267
Mox20I	TGGCCA	3 cut(s)	495, 933, 1727
MroXI	GAANNNNTTC	1 cut(s)	353
MscI	TGGCCA	3 cut(s)	495, 933, 1727
MseI	TTAA	8 cut(s)	168, 387, 776, 1242, 1581, 1622, 1767, 1773
MslI	CAYNNNNRTG	3 cut(s)	207, 1529, 2361
Msp20I	TGGCCA	3 cut(s)	495, 933, 1727
MspCI	CTTAAG	1 cut(s)	1766
MspI	CCGG	2 cut(s)	2261, 2471
MspR9I	CCNGG	6 cut(s)	713, 1565, 1653, 2015, 2262, 2660
MunI	CAATTG	2 cut(s)	1902, 2399
Mva1269I	GAATGC	1 cut(s)	898
MvaI	CCWGG	5 cut(s)	713, 1565, 1653, 2015, 2660
MvnI	CGCG	2 cut(s)	418, 1153
MwoI	GCNNNNNNNGC	5 cut(s)	1159, 1733, 1916, 2591, 2597
NciI	CCSGG	1 cut(s)	2262
NdeI	CATATG	2 cut(s)	246, 2681
NdeII	GATC	7 cut(s)	35, 380, 1429, 1648, 1674, 1810, 2330
NlaIII	CATG	9 cut(s)	490, 632, 1123, 1430, 1844, 2011, 2250, 2519, 2654
NlaIV	GGNNCC	7 cut(s)	399, 1201, 1817, 1858, 2201, 2332, 2466
NmuCI	GTSAC	2 cut(s)	1699, 2604
NsbI	TGCGCA	1 cut(s)	1881
NspI	RCATGY	3 cut(s)	2011, 2250, 2654
NspV	TTCGAA	1 cut(s)	50
OliI	CACNNNNGTG	1 cut(s)	207
PaeI	GCATGC	1 cut(s)	2654
PaeR7I	CTCGAG	1 cut(s)	2538
PagI	TCATGA	1 cut(s)	1426
PciI	ACATGT	1 cut(s)	2007
PciSI	GCTCTTC	1 cut(s)	1497
PctI	GAATGC	1 cut(s)	898
PdmI	GAANNNNTTC	1 cut(s)	353
PfeI	GAWTC	8 cut(s)	94, 553, 659, 990, 1357, 1491, 1942, 2543
PinAI	ACCGGT	1 cut(s)	2470
PkrI	GCNGC	9 cut(s)	1117, 1712, 1879, 1909, 1954, 2199, 2587, 2590, 2593
PleI	GAGTC	3 cut(s)	1678, 2093, 2247
PpsI	GAGTC	3 cut(s)	1678, 2093, 2247
PpuMI	RGGWCCY	1 cut(s)	683
PscI	ACATGT	1 cut(s)	2007
PshBI	ATTAAT	2 cut(s)	1622, 1773
PsiI	TTATAA	2 cut(s)	723, 2643
Psp124BI	GAGCTC	2 cut(s)	270, 462
Psp5II	RGGWCCY	1 cut(s)	683
Psp6I	CCWGG	5 cut(s)	711, 1563, 1651, 2013, 2658
PspEI	GGTNACC	1 cut(s)	1327
PspFI	CCCAGC	3 cut(s)	1401, 1486, 1968
PspGI	CCWGG	5 cut(s)	711, 1563, 1651, 2013, 2658
PspN4I	GGNNCC	7 cut(s)	399, 1201, 1817, 1858, 2201, 2332, 2466
PspPI	GGNCC	5 cut(s)	683, 1199, 1280, 1857, 2095
PspPPI	RGGWCCY	1 cut(s)	683
PsuI	RGATCY	3 cut(s)	1648, 1674, 2330
RsaI	GTAC	8 cut(s)	328, 485, 866, 1440, 1569, 1593, 2446, 2466
RsaNI	GTAC	8 cut(s)	327, 484, 865, 1439, 1568, 1592, 2445, 2465
RseI	CAYNNNNRTG	3 cut(s)	207, 1529, 2361
SacI	GAGCTC	2 cut(s)	270, 462
SapI	GCTCTTC	1 cut(s)	1497
SaqAI	TTAA	8 cut(s)	168, 387, 776, 1242, 1581, 1622, 1767, 1773
SatI	GCNGC	9 cut(s)	1116, 1711, 1878, 1908, 1953, 2198, 2586, 2589, 2592
Sau3AI	GATC	7 cut(s)	35, 380, 1429, 1648, 1674, 1810, 2330
Sau96I	GGNCC	5 cut(s)	683, 1199, 1280, 1857, 2095
ScaI	AGTACT	1 cut(s)	328
SchI	GAGTC	3 cut(s)	1678, 2094, 2248
ScrFI	CCNGG	6 cut(s)	713, 1565, 1653, 2015, 2262, 2660
SduI	GDGCHC	3 cut(s)	270, 462, 2292
SfaNI	GCATC	4 cut(s)	650, 831, 1545, 2609
SfcI	CTRYAG	1 cut(s)	2454
Sfr274I	CTCGAG	1 cut(s)	2538
SfuI	TTCGAA	1 cut(s)	50
SinI	GGWCC	2 cut(s)	683, 1199
SlaI	CTCGAG	1 cut(s)	2538
SmiMI	CAYNNNNRTG	3 cut(s)	207, 1529, 2361
SmlI	CTYRAG	3 cut(s)	461, 1766, 2538
SmoI	CTYRAG	3 cut(s)	461, 1766, 2538
SpeI	ACTAGT	1 cut(s)	605
SphI	GCATGC	1 cut(s)	2654
SsiI	CCGC	7 cut(s)	367, 418, 941, 1151, 1907, 2197, 2506
SspMI	CTAG	7 cut(s)	606, 645, 1545, 1641, 2129, 2219, 2627
SstI	GAGCTC	2 cut(s)	270, 462
StyD4I	CCNGG	6 cut(s)	711, 1563, 1651, 2013, 2260, 2658
StyI	CCWWGG	4 cut(s)	622, 679, 944, 1519
TaqI	TCGA	8 cut(s)	28, 50, 786, 963, 1432, 1963, 2526, 2539
TatI	WGTACW	5 cut(s)	326, 483, 864, 1438, 2444
TauI	GCSGC	2 cut(s)	1910, 2200
TfiI	GAWTC	8 cut(s)	94, 553, 659, 990, 1357, 1491, 1942, 2543
Tru1I	TTAA	8 cut(s)	168, 387, 776, 1242, 1581, 1622, 1767, 1773
Tru9I	TTAA	8 cut(s)	168, 387, 776, 1242, 1581, 1622, 1767, 1773
TscAI	CASTG	7 cut(s)	364, 369, 382, 1712, 1927, 2095, 2622
TseFI	GTSAC	2 cut(s)	1699, 2604
TseI	GCWGC	7 cut(s)	1115, 1710, 1877, 1952, 2585, 2588, 2591
Tsp45I	GTSAC	2 cut(s)	1699, 2604
TspGWI	ACGGA	3 cut(s)	135, 323, 2066
TspRI	CASTG	7 cut(s)	364, 369, 382, 1712, 1927, 2095, 2622
Vha464I	CTTAAG	1 cut(s)	1766
VneI	GTGCAC	1 cut(s)	2288
VpaK11BI	GGWCC	2 cut(s)	683, 1199
VspI	ATTAAT	2 cut(s)	1622, 1773
XagI	CCTNNNNNAGG	1 cut(s)	2437
XapI	RAATTY	3 cut(s)	45, 252, 1225
XbaI	TCTAGA	3 cut(s)	644, 2218, 2626
XceI	RCATGY	3 cut(s)	2011, 2250, 2654
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1398
XhoI	CTCGAG	1 cut(s)	2538
XmiI	GTMKAC	2 cut(s)	921, 2475
XmnI	GAANNNNTTC	1 cut(s)	353
XspI	CTAG	7 cut(s)	606, 645, 1545, 1641, 2129, 2219, 2627
ZrmI	AGTACT	1 cut(s)	328

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.