Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0007187

Physical Location & Seq

Genomic Coordinates Reverse (-)

14462779 .. 14467716

Gene Structure 4938 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG141900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2484 bp

ATGCACTATGATGTTAATGTCAAACCAGTGAATGCTACAAGGAATGGCAGACCAGTTAGAATTATGAAGTCTGATCTTGCTGCAATCAGAAACAAGTTGTCCTCTGATAATCCTTCAAAATTTCCATTATTGATGACTGCATATGATGGTGAGAAGAATATCTTTAGTGCTGTGACATTACCCACTGGAGAGTTTAAGGTGGAGGTTCCTGAGGAAGAAGGCACACGCATAAGTTCGTATATAGTCACTATAAAACTTGTAAATGAGCTCAAGCTTTGCAAGTTGAAGGAGTACTTAAGTGGTCAGCTGTTGTCTATCCCTCGTGATATATTGCAGGGAATGGATTTAGTGATGAAGGAGAATCCTACTCGGCGGTTGATTCCTGTTGGCCGCAACTTTTACCCTGCTGAATTTAATCCAGACGATGACCTTGGACAGGGTACTGCTGCATTTAGAGGGTTTCAGCATAGTTTAAGGCTCACTTCCCAGGGTCCTGCTATGTGTCTGGACTACTCAGTCTTGGCATTTCATAAGCGCATGCCAGTTATAGATTTTCTCCATGAAAAGATAAGGAATTTTACTTTAAATAATTTCAGGAGGTTTAAGAGAGATGTTGAGAATGCTTTAAGGGGATTGAAAGTTACTGTGACTCATCGTCCAACCAAGCAGAAGTATGTTATCAAGGGGCTAACTGATAGGAATGCAGGGGACATTACATTTGAAGCTGTTGATGTTGATGGCCAGGTTCCACCTAGAAGGCTTAGACTTGTTGATTATTTCAGAGACAAATACAAGGATATCAAGTACAAAGACATTCCCTGCTTAGATTTAGGGAAAAATGGTAGGAGGAATGATACACCGTTGGAATTTTGTGTCTTAGTTGAAGGACAAAGATACCCTAAGGAGCAGTTGGGCAGAGATGCAGCCATTATGTTGAAGAACTTGTCATTGGTTACACCAGGGGTTAGAGAGAACAACATACGCAACATGGCACGGTCGGGAGATGGACCTTGTGGTGGCGGCATTATCCAGAACTTTGGAATTGAAGTCAACATGAATATGACACAAGTAACAGGGCGTGTCATTGGCCCACCTGAGTTGAAGCTGGGGGATTTTGGTGGCAAGGTGACTAAAGTAAAAGTTGACAATGAGAAATGTCACTGGAATCTAGTTGGCAAGTCATTAGTGGAAGGAAAACCAATAGCTCGCTGGGCTGTGCTTGACTTTAGCTGTTTTGATCAAGACTGTTATGATCGGGACAAACTGGACCCTTATCAATTTATCCCAAAGCTCATCACTCGGTGCAACAAATTGGGAATGAGAATGGAAGAGCCTCGCTTGTATGAAACCTCTTCCATGAGACCATTTTCTAGCGTCAACTTGCTTCGGGAACTGCTTGAAACTGTTAATGGGAAGGTACTTCAAGAAGGCAAGGGCCACTTACAACTACTTGTTTGTGTAATGTCTAGAAAAGACCCTGGTTACAAGTATCTGAAGTGGATCTGTGAGACTCAAATTGGTATAGTGACGCAGTGCTGCTTGTCAAAGATGGCCACCAGGCCCAGTGACCAGTTTCTTGCAAATCTTGCACTGAAGATCAATGCGAAGCTTGGAGGCAGTAATGTAGAGCTGATTGACCGGCTCCCGCTTTTTGAGGGTGCTGGCCCTGTTATGTTTGTGGGGGCTGACGTTAATCATCCTGCGGCTCGGAACACAACAAGTCCATCAATAGCAGCTGTGGTTGCCACTATAAACTGGCCTGCTGTGAATCGTTATGCTGCACGAGTCCGACCCCAGTACCATCGTACTGAGAGCATATCGAATTTTGGAGACATGGTTTTGGAGCTTGTTGAATCGTATTTTAGAATAAATAAAGTGAAGCCTGAAAAAATTGTTGTTTTCCGAGATGGTGTAAGTGAGGGGCAATTTGATATGGTTCTCAATGAAGAGTTACTAGATCTGAAGAGGGCTTTGGGAACTATAAACTACTTTCCAACCATGACTCTGATTGTTGCTCAAAAGCGGCATCATACTCGGCTGTTTCAGGAGAATGGACGTTCCAATGTATCTCCAGGAACTGTTGTGGACACAAGGATTGTGCACCCATTTGAGTTTGACTTCTATCTCTGCAGTCACTATGGAAGCCTTGGGACAAGCAAGCCGACACACTACTATGTTCTTTGGGATGAGCACAGTTTTACTTCCGACCAGTTGCAGAAGCTCATATATGACTTGTGCTTTACCTTTGCAAGGTGCACGAAACCTGTCTCTTTGGTTCCGCCAGTGTACTATGCTGACCTTGTGGCCTACAGAGGGAGATTGTACCATGAGACTATGACCGCAGGACTGTCTCCAGGTTCCGTAACATCCTCCTCCTCCTCAGCATCATCATCATCGGCACCTTCAACACTGTCGGTGGGTTCTCTGGATGACAGGTTTTATAGATTGCATGTTGACCTGGAAAACATTATGTTTTTCATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0016032 GO:0019048 GO:0035197 GO:0035821 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0061980 GO:0097159 GO:0098542 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

827

Amino Acids

93.45

Weight (kDa)

9.06

Isoelectric Point (pI)

35.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	1 - 118	5e-15	N-terminal domain of argonaute
ArgoL1	PF08699	128 - 178	4e-11	Argonaute linker 1 domain
PAZ	PF02170	196 - 305	7.7e-22	PAZ domain
ArgoL2	PF16488	323 - 368	2.4e-07	Argonaute linker 2 domain
Piwi	PF02171	484 - 773	7e-93	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31280 AT1G31290 AT1G31290 AT1G31290
fragaria_vesca	FvH4_3g11980
malus_domestica	MD05G1256100.v1.1 MD10G1236300.v1.1 MD10G1236400.v1.1
prunus_persica	Prupe.4G108000_v2.0.a1 Prupe.4G108100_v2.0.a1
pyrus_communis	pycom05g23500 pycom05g23510 pycom05g23520 pycom10g19770 pycom10g19780
rosa_chinensis	RchiOBHm_Chr5g0019581
rosa_laevigata	RLG00000032457
rosa_roxburghii	Rroxscaffold_1G00058500
rosa_rugosa	Rorug05G0052100 Rorug05G0052200
rosa_samantha	Rh5AG142700 Rh5BG141200 Rh5CG153300 Rh5DG141900
rosa_wichuraiana	Rw5G012630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	515
AccB1I	GGYRCC	1 cut(s)	2398
AciI	CCGC	8 cut(s)	373, 391, 1020, 1646, 1703, 2023, 2279, 2340
AclWI	GGATC	1 cut(s)	1508
AcoI	YGGCCR	3 cut(s)	388, 739, 1551
AcsI	RAATTY	5 cut(s)	119, 410, 574, 866, 1822
AcuI	CTGAAG	3 cut(s)	1514, 1613, 1982
AdeI	CACNNNGTG	1 cut(s)	1302
AfaI	GTAC	8 cut(s)	293, 442, 806, 1419, 1799, 1807, 2288, 2324
AfiI	CCNNNNNNNGG	4 cut(s)	436, 1016, 2250, 2313
AflII	CTTAAG	1 cut(s)	295
AhdI	GACNNNNNGTC	1 cut(s)	654
AjnI	CCWGG	8 cut(s)	486, 741, 958, 1477, 1556, 2071, 2353, 2457
AjuI	GAANNNNNNNTTGG	4 cut(s)	932, 964, 1955, 1987
Alw21I	GWGCWC	4 cut(s)	270, 2103, 2193, 2258
Alw26I	GTCTC	7 cut(s)	777, 1354, 1502, 1824, 2272, 2324, 2355
Alw44I	GTGCAC	2 cut(s)	2099, 2254
AlwI	GGATC	1 cut(s)	1508
AlwNI	CAGNNNCTG	1 cut(s)	2078
AoxI	GGCC	9 cut(s)	388, 739, 1087, 1435, 1551, 1559, 1663, 1757, 2304
ApaLI	GTGCAC	2 cut(s)	2099, 2254
ApeKI	GCWGC	6 cut(s)	80, 446, 923, 1536, 1733, 1778
ApoI	RAATTY	5 cut(s)	119, 410, 574, 866, 1822
ArsI	GACNNNNNNTTYG	2 cut(s)	701, 733
AspLEI	GCGC	1 cut(s)	537
AspS9I	GGNCC	7 cut(s)	491, 1007, 1088, 1267, 1435, 1560, 1664
AsuHPI	GGTGA	2 cut(s)	161, 1138
AvaII	GGWCC	3 cut(s)	491, 1007, 1267
AxyI	CCTNAGG	2 cut(s)	210, 900
BaeGI	GKGCMC	2 cut(s)	2103, 2258
BaeI	ACNNNNGTAYC	2 cut(s)	1781, 1814
BalI	TGGCCA	2 cut(s)	741, 1553
BanI	GGYRCC	1 cut(s)	2398
BanII	GRGCYC	1 cut(s)	270
BauI	CACGAG	2 cut(s)	321, 1782
Bbv12I	GWGCWC	4 cut(s)	270, 2103, 2193, 2258
BbvCI	CCTCAGC	1 cut(s)	2380
BbvI	GCAGC	6 cut(s)	67, 433, 935, 1523, 1745, 1765
BccI	CCATC	7 cut(s)	140, 731, 998, 1543, 1732, 1809, 1901
BcgI	CGANNNNNNTGC	2 cut(s)	2015, 2049
BciT130I	CCWGG	8 cut(s)	488, 743, 960, 1479, 1558, 2073, 2355, 2459
BclI	TGATCA	1 cut(s)	1237
BcoDI	GTCTC	7 cut(s)	777, 1354, 1502, 1824, 2272, 2324, 2355
BfaI	CTAG	5 cut(s)	753, 1169, 1371, 1467, 1955
BfmI	CTRYAG	2 cut(s)	2128, 2308
BfrI	CTTAAG	1 cut(s)	295
BglII	AGATCT	1 cut(s)	1957
BmcAI	AGTACT	1 cut(s)	293
Bme1390I	CCNGG	8 cut(s)	488, 743, 960, 1479, 1558, 2073, 2355, 2459
Bme18I	GGWCC	3 cut(s)	491, 1007, 1267
BmeRI	GACNNNNNGTC	1 cut(s)	654
BmgT120I	GGNCC	7 cut(s)	491, 1007, 1088, 1267, 1435, 1560, 1664
BmiI	GGNNCC	8 cut(s)	207, 492, 747, 1269, 1643, 2277, 2359, 2400
BmrFI	CCNGG	8 cut(s)	488, 743, 960, 1479, 1558, 2073, 2355, 2459
BmrI	ACTGGG	2 cut(s)	1557, 1789
BmsI	GCATC	3 cut(s)	910, 2035, 2393
BmuI	ACTGGG	2 cut(s)	1557, 1789
BpmI	CTGGAG	3 cut(s)	207, 2055, 2337
Bpu10I	CCTNAGC	1 cut(s)	2380
BpuEI	CTTGAG	1 cut(s)	254
BsaI	GGTCTC	1 cut(s)	1354
BsaJI	CCNNGG	6 cut(s)	430, 486, 487, 959, 1477, 2146
BsaXI	ACNNNNNCTCC	2 cut(s)	1821, 1851
Bsc4I	CCNNNNNNNGG	4 cut(s)	436, 1016, 2250, 2313
Bse118I	RCCGGY	1 cut(s)	1638
Bse21I	CCTNAGG	2 cut(s)	210, 900
BseBI	CCWGG	8 cut(s)	488, 743, 960, 1479, 1558, 2073, 2355, 2459
BseDI	CCNNGG	6 cut(s)	430, 486, 487, 959, 1477, 2146
BseGI	GGATG	4 cut(s)	1696, 2191, 2366, 2434
BseLI	CCNNNNNNNGG	4 cut(s)	436, 1016, 2250, 2313
BseMII	CTCAG	5 cut(s)	201, 528, 1086, 1800, 2394
BseRI	GAGGAG	3 cut(s)	2362, 2365, 2368
BseSI	GKGCMC	2 cut(s)	2103, 2258
BseXI	GCAGC	6 cut(s)	67, 433, 935, 1523, 1745, 1765
BseYI	CCCAGC	2 cut(s)	1105, 1209
BsgI	GTGCAG	1 cut(s)	1764
Bsh1285I	CGRYCG	1 cut(s)	998
BshFI	GGCC	9 cut(s)	390, 741, 1089, 1437, 1553, 1561, 1665, 1759, 2306
BshNI	GGYRCC	1 cut(s)	2398
BsiEI	CGRYCG	1 cut(s)	998
BsiHKAI	GWGCWC	4 cut(s)	270, 2103, 2193, 2258
BsiSI	CCGG	1 cut(s)	1639
BslFI	GGGAC	3 cut(s)	722, 1271, 2164
BslI	CCNNNNNNNGG	4 cut(s)	436, 1016, 2250, 2313
BsmAI	GTCTC	7 cut(s)	777, 1354, 1502, 1824, 2272, 2324, 2355
BsmFI	GGGAC	3 cut(s)	722, 1271, 2164
BsmI	GAATGC	3 cut(s)	37, 625, 706
BsnI	GGCC	9 cut(s)	390, 741, 1089, 1437, 1553, 1561, 1665, 1759, 2306
Bso31I	GGTCTC	1 cut(s)	1354
Bsp1286I	GDGCHC	4 cut(s)	270, 2103, 2193, 2258
Bsp143I	GATC	6 cut(s)	73, 1237, 1252, 1500, 1596, 1957
BspACI	CCGC	8 cut(s)	373, 391, 1020, 1646, 1703, 2023, 2279, 2340
BspANI	GGCC	9 cut(s)	390, 741, 1089, 1437, 1553, 1561, 1665, 1759, 2306
BspCNI	CTCAG	5 cut(s)	202, 527, 1087, 1801, 2393
BspLI	GGNNCC	8 cut(s)	207, 492, 747, 1269, 1643, 2277, 2359, 2400
BspMAI	CTGCAG	1 cut(s)	2132
BspPI	GGATC	1 cut(s)	1508
BspQI	GCTCTTC	1 cut(s)	1323
BspT107I	GGYRCC	1 cut(s)	2398
BspTI	CTTAAG	1 cut(s)	295
BspTNI	GGTCTC	1 cut(s)	1354
BsrFI	RCCGGY	1 cut(s)	1638
BssAI	RCCGGY	1 cut(s)	1638
BssECI	CCNNGG	6 cut(s)	430, 486, 487, 959, 1477, 2146
BssMI	GATC	6 cut(s)	73, 1237, 1252, 1500, 1596, 1957
BssSI	CACGAG	2 cut(s)	321, 1782
BssT1I	CCWWGG	2 cut(s)	430, 2146
Bst2BI	CACGAG	2 cut(s)	321, 1782
Bst2UI	CCWGG	8 cut(s)	488, 743, 960, 1479, 1558, 2073, 2355, 2459
Bst4CI	ACNGT	9 cut(s)	646, 861, 996, 1247, 1405, 2080, 2195, 2349, 2412
Bst6I	CTCTTC	4 cut(s)	1323, 1357, 1941, 1958
BstAFI	CTTAAG	1 cut(s)	295
BstAPI	GCANNNNNTGC	1 cut(s)	1586
BstC8I	GCNNGC	5 cut(s)	539, 1207, 1663, 1761, 2159
BstDEI	CTNAG	9 cut(s)	210, 514, 761, 823, 877, 900, 1095, 1809, 2380
BstENI	CCTNNNNNAGG	2 cut(s)	434, 2248
BstF5I	GGATG	4 cut(s)	1696, 2191, 2366, 2434
BstHHI	GCGC	1 cut(s)	537
BstKTI	GATC	6 cut(s)	76, 1240, 1255, 1503, 1599, 1960
BstMAI	GTCTC	7 cut(s)	777, 1354, 1502, 1824, 2272, 2324, 2355
BstMBI	GATC	6 cut(s)	73, 1237, 1252, 1500, 1596, 1957
BstMCI	CGRYCG	1 cut(s)	998
BstMWI	GCNNNNNNNGC	3 cut(s)	1211, 1586, 1742
BstNI	CCWGG	8 cut(s)	488, 743, 960, 1479, 1558, 2073, 2355, 2459
BstNSI	RCATGY	2 cut(s)	541, 2453
BstSCI	CCNGG	8 cut(s)	486, 741, 958, 1477, 1556, 2071, 2353, 2457
BstSFI	CTRYAG	2 cut(s)	2128, 2308
BstSLI	GKGCMC	2 cut(s)	2103, 2258
BstV1I	GCAGC	6 cut(s)	67, 433, 935, 1523, 1745, 1765
BstX2I	RGATCY	2 cut(s)	1500, 1957
BstXI	CCANNNNNNTGG	1 cut(s)	1037
BstYI	RGATCY	2 cut(s)	1500, 1957
Bsu36I	CCTNAGG	2 cut(s)	210, 900
BsuRI	GGCC	9 cut(s)	390, 741, 1089, 1437, 1553, 1561, 1665, 1759, 2306
BtsCI	GGATG	4 cut(s)	1696, 2191, 2366, 2434
BtsI	GCAGTG	1 cut(s)	1538
BtsIMutI	CAGTG	8 cut(s)	33, 183, 1159, 1538, 1570, 1589, 2289, 2408
Cac8I	GCNNGC	5 cut(s)	539, 1207, 1663, 1761, 2159
CaiI	CAGNNNCTG	1 cut(s)	2078
CfoI	GCGC	1 cut(s)	537
Cfr10I	RCCGGY	1 cut(s)	1638
Cfr13I	GGNCC	7 cut(s)	491, 1007, 1088, 1267, 1435, 1560, 1664
CseI	GACGC	2 cut(s)	1363, 1537
Csp6I	GTAC	8 cut(s)	292, 441, 805, 1418, 1798, 1806, 2287, 2323
CspCI	CAANNNNNGTGG	2 cut(s)	1080, 1115
CviAII	CATG	9 cut(s)	538, 560, 988, 1054, 1357, 1834, 1999, 2327, 2450
CviQI	GTAC	8 cut(s)	292, 441, 805, 1418, 1798, 1806, 2287, 2323
DdeI	CTNAG	9 cut(s)	210, 514, 761, 823, 877, 900, 1095, 1809, 2380
DpnI	GATC	6 cut(s)	75, 1239, 1254, 1502, 1598, 1959
DpnII	GATC	6 cut(s)	73, 1237, 1252, 1500, 1596, 1957
DraI	TTTAAA	1 cut(s)	585
DraIII	CACNNNGTG	1 cut(s)	1302
DrdI	GACNNNNNNGTC	1 cut(s)	515
DriI	GACNNNNNGTC	1 cut(s)	654
DseDI	GACNNNNNNGTC	1 cut(s)	515
EaeI	YGGCCR	3 cut(s)	388, 739, 1551
Eam1104I	CTCTTC	4 cut(s)	1323, 1357, 1941, 1958
Eam1105I	GACNNNNNGTC	1 cut(s)	654
EarI	CTCTTC	4 cut(s)	1323, 1357, 1941, 1958
EciI	GGCGGA	1 cut(s)	2268
Ecl136II	GAGCTC	1 cut(s)	268
Eco130I	CCWWGG	2 cut(s)	430, 2146
Eco24I	GRGCYC	1 cut(s)	270
Eco31I	GGTCTC	1 cut(s)	1354
Eco32I	GATATC	1 cut(s)	799
Eco47I	GGWCC	3 cut(s)	491, 1007, 1267
Eco53kI	GAGCTC	1 cut(s)	268
Eco57I	CTGAAG	3 cut(s)	1514, 1613, 1982
Eco81I	CCTNAGG	2 cut(s)	210, 900
EcoICRI	GAGCTC	1 cut(s)	268
EcoNI	CCTNNNNNAGG	2 cut(s)	434, 2248
EcoO109I	RGGNCCY	1 cut(s)	491
EcoRII	CCWGG	8 cut(s)	486, 741, 958, 1477, 1556, 2071, 2353, 2457
EcoRV	GATATC	1 cut(s)	799
EcoT14I	CCWWGG	2 cut(s)	430, 2146
EcoT38I	GRGCYC	1 cut(s)	270
ErhI	CCWWGG	2 cut(s)	430, 2146
FaeI	CATG	9 cut(s)	541, 563, 991, 1057, 1360, 1837, 2002, 2330, 2453
FalI	AAGNNNNNCTT	8 cut(s)	146, 178, 278, 310, 466, 498, 1424, 1456
FaqI	GGGAC	3 cut(s)	722, 1271, 2164
FatI	CATG	9 cut(s)	537, 559, 987, 1053, 1356, 1833, 1998, 2326, 2449
FauI	CCCGC	1 cut(s)	1653
FauNDI	CATATG	1 cut(s)	142
FbaI	TGATCA	1 cut(s)	1237
FokI	GGATG	4 cut(s)	1683, 2198, 2353, 2441
FriOI	GRGCYC	1 cut(s)	270
FspBI	CTAG	5 cut(s)	753, 1169, 1371, 1467, 1955
GlaI	GCGC	1 cut(s)	536
GsaI	CCCAGC	2 cut(s)	1109, 1213
GsuI	CTGGAG	3 cut(s)	207, 2055, 2337
HaeIII	GGCC	9 cut(s)	390, 741, 1089, 1437, 1553, 1561, 1665, 1759, 2306
HapII	CCGG	1 cut(s)	1639
HgaI	GACGC	2 cut(s)	1363, 1537
HhaI	GCGC	1 cut(s)	537
Hin1II	CATG	9 cut(s)	541, 563, 991, 1057, 1360, 1837, 2002, 2330, 2453
Hin6I	GCGC	1 cut(s)	535
HinP1I	GCGC	1 cut(s)	535
HincII	GTYRAC	4 cut(s)	1051, 1144, 1378, 2455
HindII	GTYRAC	4 cut(s)	1051, 1144, 1378, 2455
HindIII	AAGCTT	2 cut(s)	272, 1607
HinfI	GANTC	9 cut(s)	361, 379, 649, 1165, 1510, 1768, 1785, 1853, 2002
HpaII	CCGG	1 cut(s)	1639
HphI	GGTGA	2 cut(s)	161, 1138
Hpy166II	GTNNAC	8 cut(s)	1051, 1144, 1378, 2086, 2101, 2256, 2287, 2455
Hpy8I	GTNNAC	8 cut(s)	1051, 1144, 1378, 2086, 2101, 2256, 2287, 2455
HpyCH4III	ACNGT	9 cut(s)	646, 861, 996, 1247, 1405, 2080, 2195, 2349, 2412
HpyCH4IV	ACGT	2 cut(s)	1689, 2056
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1211, 1586, 1742
HpyF3I	CTNAG	9 cut(s)	210, 514, 761, 823, 877, 900, 1095, 1809, 2380
HpySE526I	ACGT	2 cut(s)	1689, 2056
Hsp92II	CATG	9 cut(s)	541, 563, 991, 1057, 1360, 1837, 2002, 2330, 2453
HspAI	GCGC	1 cut(s)	535
Ksp22I	TGATCA	1 cut(s)	1237
Kzo9I	GATC	6 cut(s)	73, 1237, 1252, 1500, 1596, 1957
LguI	GCTCTTC	1 cut(s)	1323
LmnI	GCTCC	3 cut(s)	904, 1647, 1843
Lsp1109I	GCAGC	6 cut(s)	67, 433, 935, 1523, 1745, 1765
LweI	GCATC	3 cut(s)	910, 2035, 2393
MaeI	CTAG	5 cut(s)	753, 1169, 1371, 1467, 1955
MaeII	ACGT	2 cut(s)	1689, 2056
MalI	GATC	6 cut(s)	75, 1239, 1254, 1502, 1598, 1959
MboI	GATC	6 cut(s)	73, 1237, 1252, 1500, 1596, 1957
MboII	GAAGA	8 cut(s)	166, 227, 949, 1340, 1344, 1606, 1958, 1975
MflI	RGATCY	2 cut(s)	1500, 1957
MhlI	GDGCHC	4 cut(s)	270, 2103, 2193, 2258
MlsI	TGGCCA	2 cut(s)	741, 1553
MluNI	TGGCCA	2 cut(s)	741, 1553
MlyI	GAGTC	4 cut(s)	643, 1504, 1794, 1996
MmeI	TCCRAC	5 cut(s)	683, 843, 1813, 2018, 2229
Mox20I	TGGCCA	2 cut(s)	741, 1553
MscI	TGGCCA	2 cut(s)	741, 1553
MslI	CAYNNNNRTG	3 cut(s)	9, 1058, 2172
Msp20I	TGGCCA	2 cut(s)	741, 1553
MspA1I	CMGCKG	2 cut(s)	307, 1736
MspCI	CTTAAG	1 cut(s)	295
MspI	CCGG	1 cut(s)	1639
MspR9I	CCNGG	8 cut(s)	488, 743, 960, 1479, 1558, 2073, 2355, 2459
Mva1269I	GAATGC	3 cut(s)	37, 625, 706
MvaI	CCWGG	8 cut(s)	488, 743, 960, 1479, 1558, 2073, 2355, 2459
MwoI	GCNNNNNNNGC	3 cut(s)	1211, 1586, 1742
NdeI	CATATG	1 cut(s)	142
NdeII	GATC	6 cut(s)	73, 1237, 1252, 1500, 1596, 1957
NlaIII	CATG	9 cut(s)	541, 563, 991, 1057, 1360, 1837, 2002, 2330, 2453
NlaIV	GGNNCC	8 cut(s)	207, 492, 747, 1269, 1643, 2277, 2359, 2400
NmeAIII	GCCGAG	2 cut(s)	349, 2014
NmuCI	GTSAC	8 cut(s)	172, 244, 646, 1126, 1157, 1525, 1565, 2132
NspI	RCATGY	2 cut(s)	541, 2453
PaeI	GCATGC	1 cut(s)	541
PasI	CCCWGGG	1 cut(s)	487
PciSI	GCTCTTC	1 cut(s)	1323
PctI	GAATGC	3 cut(s)	37, 625, 706
PfeI	GAWTC	5 cut(s)	361, 379, 1165, 1768, 1853
PfoI	TCCNGGA	1 cut(s)	2071
PleI	GAGTC	4 cut(s)	643, 1504, 1793, 1996
PpsI	GAGTC	4 cut(s)	643, 1504, 1793, 1996
PpuMI	RGGWCCY	1 cut(s)	491
Psp124BI	GAGCTC	1 cut(s)	270
Psp5II	RGGWCCY	1 cut(s)	491
Psp6I	CCWGG	8 cut(s)	486, 741, 958, 1477, 1556, 2071, 2353, 2457
PspFI	CCCAGC	2 cut(s)	1105, 1209
PspGI	CCWGG	8 cut(s)	486, 741, 958, 1477, 1556, 2071, 2353, 2457
PspN4I	GGNNCC	8 cut(s)	207, 492, 747, 1269, 1643, 2277, 2359, 2400
PspPI	GGNCC	7 cut(s)	491, 1007, 1088, 1267, 1435, 1560, 1664
PspPPI	RGGWCCY	1 cut(s)	491
PstI	CTGCAG	1 cut(s)	2132
PstNI	CAGNNNCTG	1 cut(s)	2078
PsuI	RGATCY	2 cut(s)	1500, 1957
PvuII	CAGCTG	2 cut(s)	307, 1736
RsaI	GTAC	8 cut(s)	293, 442, 806, 1419, 1799, 1807, 2288, 2324
RsaNI	GTAC	8 cut(s)	292, 441, 805, 1418, 1798, 1806, 2287, 2323
RseI	CAYNNNNRTG	3 cut(s)	9, 1058, 2172
SacI	GAGCTC	1 cut(s)	270
SapI	GCTCTTC	1 cut(s)	1323
Sau3AI	GATC	6 cut(s)	73, 1237, 1252, 1500, 1596, 1957
Sau96I	GGNCC	7 cut(s)	491, 1007, 1088, 1267, 1435, 1560, 1664
ScaI	AGTACT	1 cut(s)	293
SchI	GAGTC	4 cut(s)	643, 1504, 1794, 1996
ScrFI	CCNGG	8 cut(s)	488, 743, 960, 1479, 1558, 2073, 2355, 2459
SduI	GDGCHC	4 cut(s)	270, 2103, 2193, 2258
SfaNI	GCATC	3 cut(s)	910, 2035, 2393
SfcI	CTRYAG	2 cut(s)	2128, 2308
SinI	GGWCC	3 cut(s)	491, 1007, 1267
SmiMI	CAYNNNNRTG	3 cut(s)	9, 1058, 2172
SmlI	CTYRAG	2 cut(s)	269, 295
SmoI	CTYRAG	2 cut(s)	269, 295
SphI	GCATGC	1 cut(s)	541
SsiI	CCGC	8 cut(s)	373, 391, 1020, 1646, 1703, 2023, 2279, 2340
SspMI	CTAG	5 cut(s)	753, 1169, 1371, 1467, 1955
SstI	GAGCTC	1 cut(s)	270
StyD4I	CCNGG	8 cut(s)	486, 741, 958, 1477, 1556, 2071, 2353, 2457
StyI	CCWWGG	2 cut(s)	430, 2146
TaaI	ACNGT	9 cut(s)	646, 861, 996, 1247, 1405, 2080, 2195, 2349, 2412
TaiI	ACGT	2 cut(s)	1692, 2059
TaqI	TCGA	1 cut(s)	1820
TatI	WGTACW	3 cut(s)	291, 804, 2286
TauI	GCSGC	4 cut(s)	393, 1023, 1706, 2026
TfiI	GAWTC	5 cut(s)	361, 379, 1165, 1768, 1853
TscAI	CASTG	8 cut(s)	33, 190, 1166, 1538, 1570, 1596, 2289, 2415
TseFI	GTSAC	8 cut(s)	172, 244, 646, 1126, 1157, 1525, 1565, 2132
TseI	GCWGC	6 cut(s)	80, 446, 923, 1536, 1733, 1778
Tsp45I	GTSAC	8 cut(s)	172, 244, 646, 1126, 1157, 1525, 1565, 2132
TspDTI	ATGAA	8 cut(s)	80, 368, 518, 576, 1070, 1359, 1959, 2467
TspGWI	ACGGA	1 cut(s)	2350
TspRI	CASTG	8 cut(s)	33, 190, 1166, 1538, 1570, 1596, 2289, 2415
Vha464I	CTTAAG	1 cut(s)	295
VneI	GTGCAC	2 cut(s)	2099, 2254
VpaK11BI	GGWCC	3 cut(s)	491, 1007, 1267
XagI	CCTNNNNNAGG	2 cut(s)	434, 2248
XapI	RAATTY	5 cut(s)	119, 410, 574, 866, 1822
XbaI	TCTAGA	1 cut(s)	1466
XceI	RCATGY	2 cut(s)	541, 2453
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1206
XspI	CTAG	5 cut(s)	753, 1169, 1371, 1467, 1955
ZrmI	AGTACT	1 cut(s)	293

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.