Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003087

Physical Location & Seq

Genomic Coordinates Reverse (-)

42627030 .. 42634805

Gene Structure 7776 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1293700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 738 bp

ATGGCCAATGAAAACAAATCCTTGTCAATCGACTCTCCCCAGAGAAAATTGGGTAGGGGAAAGATCGAGATCAAGCGGATCGAAAACACGACCAATCGTCAAGTGACCTTCTGCAAGAGGCGCAATGGGTTGCTCAAGAAGGCCTATGAACTCTCTGTGCTCTGTGATGCAGAGGTTGCTCTCATAGTCTTCTCTAACCGTGGCCGCCTCTATGAGTATGCCAACAACAGTGTTAAAGCAACAGTTGAGAGGTACAAGAAGACATGTGCAGATTCTTCAAATACTGGATCAGTTTCTGAAGCTAGTACTCAGTACTACCAGCAAGAATCTGCAAAATTGCGTGCGCAGATCGGGAATTTGCAGAATGCCAACCGGAATATGATGGGTGATGCATTGAGTAGTATGCCTATCAAGGACCTGAAGAGCCTGGAGAGTAAACTAGAGAAAGGAATTAGCAGAATCCGATCCAAAAAGAATGAGCTCTTGTTTGCCGAAATTGAGTACATGCAGAAAAGGGAACTGGACTTGCACAACAATAACCAGCTCCTACGAGCAAAGATAGCTGAGAATGAGAGGGGCCAGCAGAACATAAATGTGATGGCCGGAGGAGGAACTTCAAGCTATGACATCCTGCAGTCTCAGCCATACGACTCTCGGAACTATTTCCAAGTGAACGCGTTACAACCCAATCATCAGTACAATCCCCGCCACGATCAGATTTCTCTTCAATTAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009791 GO:0009908 GO:0010093 GO:0010097 GO:0022414 GO:0032501 GO:0032502 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K-box SRF-TF

Protein Analysis

246

Amino Acids

27.91

Weight (kDa)

9.53

Isoelectric Point (pI)

54.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SRF-TF	PF00319	26 - 73	1.5e-26	SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box	PF01486	101 - 187	9.2e-28	K-box region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003087)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18960 AT4G18960 AT4G18960 AT4G18960
fragaria_vesca	FvH4_3g06720 FvH4_3g06720 FvH4_3g06720 FvH4_3g06720 FvH4_3g06720 FvH4_3g06720 FvH4_3g06720
malus_domestica	MD05G1293700.v1.1 MD10G1271000.v1.1
prunus_persica	Prupe.3G111300_v2.0.a1 Prupe.4G070500_v2.0.a1 Prupe.4G070500_v2.0.a1 Prupe.4G070500_v2.0.a1 Prupe.4G070500_v2.0.a1
pyrus_communis	pycom05g27100 pycom10g22680
rosa_chinensis	RchiOBHm_Chr5g0012431
rosa_laevigata	RLG00000031913
rosa_multiflora	Rmu_sc0003469.1_g000006 Rmu_sc0003469.1_g000007
rosa_roxburghii	Rroxscaffold_1G00064240
rosa_rugosa	Rorug05G0000900 Rorug05G0001000 Rorug05G0001100 Rorug05G0001200 Rorug05G0001300 Rorug05G0001300 Rorug05G0001400
rosa_samantha	Rh5AG095600 Rh5BG091800 Rh5CG104600 Rh5DG091200
rosa_wichuraiana	Rw5G008360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	345
AccII	CGCG	1 cut(s)	677
AciI	CCGC	3 cut(s)	76, 205, 706
AclWI	GGATC	3 cut(s)	86, 295, 459
AcoI	YGGCCR	3 cut(s)	3, 202, 600
AcsI	RAATTY	1 cut(s)	355
AcuI	CTGAAG	2 cut(s)	318, 440
AfaI	GTAC	5 cut(s)	254, 307, 314, 503, 698
AflIII	ACRYGT	2 cut(s)	263, 675
AgsI	TTSAA	3 cut(s)	279, 618, 728
AhdI	GACNNNNNGTC	1 cut(s)	96
AjnI	CCWGG	1 cut(s)	426
AluBI	AGCT	5 cut(s)	302, 481, 544, 563, 621
AluI	AGCT	5 cut(s)	302, 481, 544, 563, 621
Alw21I	GWGCWC	2 cut(s)	162, 483
Alw26I	GTCTC	1 cut(s)	642
AlwI	GGATC	3 cut(s)	86, 295, 459
AlwNI	CAGNNNCTG	1 cut(s)	296
AoxI	GGCC	5 cut(s)	3, 141, 202, 577, 600
ApoI	RAATTY	1 cut(s)	355
Asp700I	GAANNNNTTC	1 cut(s)	662
AspLEI	GCGC	2 cut(s)	123, 346
AspS9I	GGNCC	2 cut(s)	415, 577
AsuHPI	GGTGA	1 cut(s)	398
AvaII	GGWCC	1 cut(s)	415
BalI	TGGCCA	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	483
BbsI	GAAGAC	2 cut(s)	181, 266
Bbv12I	GWGCWC	2 cut(s)	162, 483
BccI	CCATC	2 cut(s)	376, 592
BciT130I	CCWGG	1 cut(s)	428
BcoDI	GTCTC	1 cut(s)	642
BfaI	CTAG	2 cut(s)	303, 440
BfmI	CTRYAG	1 cut(s)	632
BisI	GCNGC	1 cut(s)	205
BlsI	GCNGC	1 cut(s)	206
BmcAI	AGTACT	2 cut(s)	307, 314
Bme1390I	CCNGG	1 cut(s)	428
Bme18I	GGWCC	1 cut(s)	415
BmeRI	GACNNNNNGTC	1 cut(s)	96
BmgT120I	GGNCC	2 cut(s)	415, 577
BmiI	GGNNCC	1 cut(s)	578
BmrFI	CCNGG	1 cut(s)	428
BmsI	GCATC	2 cut(s)	157, 379
BpiI	GAAGAC	2 cut(s)	181, 266
BpmI	CTGGAG	1 cut(s)	449
BpuEI	CTTGAG	1 cut(s)	119
BsaBI	GATNNNNATC	1 cut(s)	68
BsaJI	CCNNGG	1 cut(s)	199
BsaWI	WCCGGW	1 cut(s)	372
Bse1I	ACTGG	2 cut(s)	289, 525
Bse3DI	GCAATG	1 cut(s)	130
Bse8I	GATNNNNATC	1 cut(s)	68
BseBI	CCWGG	1 cut(s)	428
BseDI	CCNNGG	1 cut(s)	199
BseGI	GGATG	1 cut(s)	627
BseJI	GATNNNNATC	1 cut(s)	68
BseMI	GCAATG	1 cut(s)	130
BseMII	CTCAG	3 cut(s)	323, 555, 653
BseNI	ACTGG	2 cut(s)	289, 525
BseRI	GAGGAG	1 cut(s)	621
BsgI	GTGCAG	1 cut(s)	288
Bsh1236I	CGCG	1 cut(s)	677
BshFI	GGCC	5 cut(s)	5, 143, 204, 579, 602
BsiHKAI	GWGCWC	2 cut(s)	162, 483
BsiSI	CCGG	2 cut(s)	373, 603
BsmAI	GTCTC	1 cut(s)	642
BsmI	GAATGC	1 cut(s)	370
BsnI	GGCC	5 cut(s)	5, 143, 204, 579, 602
Bsp1286I	GDGCHC	2 cut(s)	162, 483
Bsp143I	GATC	7 cut(s)	63, 69, 78, 287, 348, 464, 712
BspACI	CCGC	3 cut(s)	76, 205, 706
BspANI	GGCC	5 cut(s)	5, 143, 204, 579, 602
BspCNI	CTCAG	3 cut(s)	322, 556, 652
BspFNI	CGCG	1 cut(s)	677
BspLI	GGNNCC	1 cut(s)	578
BspMAI	CTGCAG	1 cut(s)	636
BspPI	GGATC	3 cut(s)	86, 295, 459
BspQI	GCTCTTC	1 cut(s)	416
BsrDI	GCAATG	1 cut(s)	130
BsrI	ACTGG	2 cut(s)	289, 525
BssECI	CCNNGG	1 cut(s)	199
BssMI	GATC	7 cut(s)	63, 69, 78, 287, 348, 464, 712
Bst2UI	CCWGG	1 cut(s)	428
Bst4CI	ACNGT	3 cut(s)	200, 230, 244
Bst6I	CTCTTC	2 cut(s)	416, 729
BstAPI	GCANNNNNTGC	1 cut(s)	176
BstC8I	GCNNGC	2 cut(s)	342, 581
BstDEI	CTNAG	3 cut(s)	309, 564, 639
BstDSI	CCRYGG	1 cut(s)	199
BstF5I	GGATG	1 cut(s)	627
BstFNI	CGCG	1 cut(s)	677
BstHHI	GCGC	2 cut(s)	123, 346
BstKTI	GATC	7 cut(s)	66, 72, 81, 290, 351, 467, 715
BstMAI	GTCTC	1 cut(s)	642
BstMBI	GATC	7 cut(s)	63, 69, 78, 287, 348, 464, 712
BstMWI	GCNNNNNNNGC	4 cut(s)	120, 176, 560, 640
BstNI	CCWGG	1 cut(s)	428
BstNSI	RCATGY	2 cut(s)	267, 508
BstSCI	CCNGG	1 cut(s)	426
BstSFI	CTRYAG	1 cut(s)	632
BstUI	CGCG	1 cut(s)	677
BstV2I	GAAGAC	2 cut(s)	181, 266
BsuRI	GGCC	5 cut(s)	5, 143, 204, 579, 602
BtgI	CCRYGG	1 cut(s)	199
BtsCI	GGATG	1 cut(s)	627
BtsIMutI	CAGTG	1 cut(s)	235
Cac8I	GCNNGC	2 cut(s)	342, 581
CaiI	CAGNNNCTG	1 cut(s)	296
CfoI	GCGC	2 cut(s)	123, 346
Cfr13I	GGNCC	2 cut(s)	415, 577
Csp6I	GTAC	5 cut(s)	253, 306, 313, 502, 697
CviAII	CATG	2 cut(s)	264, 505
CviQI	GTAC	5 cut(s)	253, 306, 313, 502, 697
DdeI	CTNAG	3 cut(s)	309, 564, 639
DpnI	GATC	7 cut(s)	65, 71, 80, 289, 350, 466, 714
DpnII	GATC	7 cut(s)	63, 69, 78, 287, 348, 464, 712
DriI	GACNNNNNGTC	1 cut(s)	96
EaeI	YGGCCR	3 cut(s)	3, 202, 600
Eam1104I	CTCTTC	2 cut(s)	416, 729
Eam1105I	GACNNNNNGTC	1 cut(s)	96
EarI	CTCTTC	2 cut(s)	416, 729
Ecl136II	GAGCTC	1 cut(s)	481
Eco147I	AGGCCT	1 cut(s)	143
Eco24I	GRGCYC	1 cut(s)	483
Eco47I	GGWCC	1 cut(s)	415
Eco53kI	GAGCTC	1 cut(s)	481
Eco57I	CTGAAG	2 cut(s)	318, 440
EcoICRI	GAGCTC	1 cut(s)	481
EcoO109I	RGGNCCY	1 cut(s)	415
EcoRII	CCWGG	1 cut(s)	426
EcoT22I	ATGCAT	1 cut(s)	394
EcoT38I	GRGCYC	1 cut(s)	483
FaeI	CATG	2 cut(s)	267, 508
FatI	CATG	2 cut(s)	263, 504
FauI	CCCGC	1 cut(s)	713
Fnu4HI	GCNGC	1 cut(s)	205
FokI	GGATG	1 cut(s)	614
FriOI	GRGCYC	1 cut(s)	483
Fsp4HI	GCNGC	1 cut(s)	205
FspBI	CTAG	2 cut(s)	303, 440
FspI	TGCGCA	1 cut(s)	345
GlaI	GCGC	2 cut(s)	122, 345
GluI	GCNGC	1 cut(s)	205
GsuI	CTGGAG	1 cut(s)	449
HaeIII	GGCC	5 cut(s)	5, 143, 204, 579, 602
HapII	CCGG	2 cut(s)	373, 603
HhaI	GCGC	2 cut(s)	123, 346
Hin1II	CATG	2 cut(s)	267, 508
Hin6I	GCGC	2 cut(s)	121, 344
HinP1I	GCGC	2 cut(s)	121, 344
HinfI	GANTC	5 cut(s)	32, 272, 326, 459, 650
HpaII	CCGG	2 cut(s)	373, 603
HphI	GGTGA	1 cut(s)	398
Hpy166II	GTNNAC	2 cut(s)	437, 673
Hpy188I	TCNGA	4 cut(s)	298, 464, 657, 717
Hpy188III	TCNNGA	3 cut(s)	67, 136, 352
Hpy8I	GTNNAC	2 cut(s)	437, 673
HpyAV	CCTTC	2 cut(s)	118, 133
HpyCH4III	ACNGT	3 cut(s)	200, 230, 244
HpyCH4V	TGCA	9 cut(s)	114, 170, 269, 332, 361, 392, 508, 529, 634
HpyF10VI	GCNNNNNNNGC	4 cut(s)	120, 176, 560, 640
HpyF3I	CTNAG	3 cut(s)	309, 564, 639
Hsp92II	CATG	2 cut(s)	267, 508
HspAI	GCGC	2 cut(s)	121, 344
Kzo9I	GATC	7 cut(s)	63, 69, 78, 287, 348, 464, 712
LguI	GCTCTTC	1 cut(s)	416
LmnI	GCTCC	1 cut(s)	549
LweI	GCATC	2 cut(s)	157, 379
MaeI	CTAG	2 cut(s)	303, 440
MaeIII	GTNAC	2 cut(s)	103, 678
MalI	GATC	7 cut(s)	65, 71, 80, 289, 350, 466, 714
MboI	GATC	7 cut(s)	63, 69, 78, 287, 348, 464, 712
MboII	GAAGA	5 cut(s)	181, 267, 271, 433, 716
MhlI	GDGCHC	2 cut(s)	162, 483
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	6 cut(s)	47, 335, 355, 450, 495, 728
MluI	ACGCGT	1 cut(s)	675
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	26, 644
MnlI	CCTC	7 cut(s)	111, 166, 218, 243, 567, 599, 602
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	394
MroXI	GAANNNNTTC	1 cut(s)	662
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	1 cut(s)	234
MslI	CAYNNNNRTG	1 cut(s)	593
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	2 cut(s)	373, 603
MspR9I	CCNGG	1 cut(s)	428
Mva1269I	GAATGC	1 cut(s)	370
MvaI	CCWGG	1 cut(s)	428
MvnI	CGCG	1 cut(s)	677
MwoI	GCNNNNNNNGC	4 cut(s)	120, 176, 560, 640
NdeII	GATC	7 cut(s)	63, 69, 78, 287, 348, 464, 712
NlaIII	CATG	2 cut(s)	267, 508
NlaIV	GGNNCC	1 cut(s)	578
NmuCI	GTSAC	1 cut(s)	103
NsbI	TGCGCA	1 cut(s)	345
NsiI	ATGCAT	1 cut(s)	394
NspI	RCATGY	2 cut(s)	267, 508
PceI	AGGCCT	1 cut(s)	143
PciI	ACATGT	1 cut(s)	263
PciSI	GCTCTTC	1 cut(s)	416
PctI	GAATGC	1 cut(s)	370
PdmI	GAANNNNTTC	1 cut(s)	662
PfeI	GAWTC	3 cut(s)	272, 326, 459
PkrI	GCNGC	1 cut(s)	206
PleI	GAGTC	2 cut(s)	26, 644
PpsI	GAGTC	2 cut(s)	26, 644
PpuMI	RGGWCCY	1 cut(s)	415
PscI	ACATGT	1 cut(s)	263
Psp124BI	GAGCTC	1 cut(s)	483
Psp5II	RGGWCCY	1 cut(s)	415
Psp6I	CCWGG	1 cut(s)	426
PspGI	CCWGG	1 cut(s)	426
PspN4I	GGNNCC	1 cut(s)	578
PspPI	GGNCC	2 cut(s)	415, 577
PspPPI	RGGWCCY	1 cut(s)	415
PstI	CTGCAG	1 cut(s)	636
PstNI	CAGNNNCTG	1 cut(s)	296
RsaI	GTAC	5 cut(s)	254, 307, 314, 503, 698
RsaNI	GTAC	5 cut(s)	253, 306, 313, 502, 697
RseI	CAYNNNNRTG	1 cut(s)	593
SacI	GAGCTC	1 cut(s)	483
SapI	GCTCTTC	1 cut(s)	416
SaqAI	TTAA	1 cut(s)	234
SatI	GCNGC	1 cut(s)	205
Sau3AI	GATC	7 cut(s)	63, 69, 78, 287, 348, 464, 712
Sau96I	GGNCC	2 cut(s)	415, 577
ScaI	AGTACT	2 cut(s)	307, 314
SchI	GAGTC	2 cut(s)	26, 644
ScrFI	CCNGG	1 cut(s)	428
SduI	GDGCHC	2 cut(s)	162, 483
SetI	ASST	9 cut(s)	110, 177, 254, 304, 420, 483, 546, 565, 623
SfaNI	GCATC	2 cut(s)	157, 379
SfcI	CTRYAG	1 cut(s)	632
SinI	GGWCC	1 cut(s)	415
SmiMI	CAYNNNNRTG	1 cut(s)	593
SmlI	CTYRAG	1 cut(s)	134
SmoI	CTYRAG	1 cut(s)	134
Sse9I	AATT	6 cut(s)	47, 335, 355, 450, 495, 728
SseBI	AGGCCT	1 cut(s)	143
SsiI	CCGC	3 cut(s)	76, 205, 706
SspMI	CTAG	2 cut(s)	303, 440
SstI	GAGCTC	1 cut(s)	483
StuI	AGGCCT	1 cut(s)	143
StyD4I	CCNGG	1 cut(s)	426
TaaI	ACNGT	3 cut(s)	200, 230, 244
TaqI	TCGA	3 cut(s)	30, 66, 81
TasI	AATT	6 cut(s)	47, 335, 355, 450, 495, 728
TatI	WGTACW	4 cut(s)	305, 312, 501, 696
TauI	GCSGC	1 cut(s)	207
TfiI	GAWTC	3 cut(s)	272, 326, 459
Tru1I	TTAA	1 cut(s)	234
Tru9I	TTAA	1 cut(s)	234
TscAI	CASTG	1 cut(s)	235
TseFI	GTSAC	1 cut(s)	103
Tsp45I	GTSAC	1 cut(s)	103
TspDTI	ATGAA	2 cut(s)	24, 162
TspRI	CASTG	1 cut(s)	235
VpaK11BI	GGWCC	1 cut(s)	415
XapI	RAATTY	1 cut(s)	355
XceI	RCATGY	2 cut(s)	267, 508
XmnI	GAANNNNTTC	1 cut(s)	662
XspI	CTAG	2 cut(s)	303, 440
ZrmI	AGTACT	2 cut(s)	307, 314
Zsp2I	ATGCAT	1 cut(s)	394

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.