MD05G1306600.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001798
Physical Location & Seq
Reverse (-)
43866535 .. 43871462
4928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1306600.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGGAGAAGCCCGGCAGTACGAGAAACGTGGGAGGAATCGAAGAGAACATATTAGCGATCCTCGATTCTTCCGAAGCTAAAGACAATCAGGATGCTTACGAGGATAGAATCACTTTTCTGGAAGCCGTTCGCGCTGCTTCCATTGTACCGAAAGACGGAACTCCACCTACCTATGCCATGTTTGAGGCAATCTTCTCCATCTTGAGGATTGGGAAGTCTCTAGAATTGACCATGGAAAGTTTTCGGCTTTTGAATGAGTTAGATAAGCGTTTTCCTCGGATATATTCTTCCGATGTAGATAATTCAACATCATCCAGTAGCGTGCCCGAATTAATCGTGGTTGAAGAGGCTTGGTATCCCTTCGCTTCCTCCGAAAATGCTAGTAATGAGAGGGGAGCTACTAATAACTCTGGGGGACCAGTTGACTCCTCTGGTTTCCAACTTTTGATACAAGACCTTGCTGATGCAACTGATGAAGCAAACTTCCAAGCATCAGAAACAAAGACTTTAGCAAACATGCTATTGTTTCAATACCTAATTAATGTTTTGGAATGGGACTTTCTACCTCGTAACCGTATCTATAAAGAAACCATGAACTGGGTAGTTCTGAGAGAGTCGTTAATCAACATGCTTCTGGTGTCAAGGAAAGTAAACCAGAAAAGCTTGACGAAGGATTGCTTGACCATTATGTGTAACCTGTATCAAACCCATGCTGGATTCACCGATGATCTGATATGTTCAAAGAAATCTGTGGCACAACCCGCTGAAAAATTTGATGCTGCTGCTGCAATTGCTTTACTCGAGATAGGAAATAATACTTGCATAGCCATGCAGAAGTTTCTAGTAATTATTATGGAGCTTGATGTGTCCAAGCAGAGTGCAGATATCCAAGGTTCTACTACCCGAGCTGATGGTATCAGATCTCCTCTGTTGGAGATAATTCTGGAGGAACTAACGTACAATAAAGATATTCTTGACCCATTTCTCCAGGTTTTCGATGAACCTAAATGGAAGCTTGAGATAGTTGTTAAGTACTTGTGGAAATATATTGCTAAACCTTCCATTCGCACTCGTAGGTCAAATGGTCCTCCAGATGATGATGCATCTTTCAACGGAGCGTTGAAGTGCTTTTCAAATATCACCAGCGCAAAAAGTACCATAAAGAAGATTAGGACAGAAGTAGTGCAGTTGCTTTTAGCACATGGATTTCAGGCTCATTTGTCGCTGCCATGTGAAAACCTTCTTGTTGGAGACACCTCTACTTCTGATGGAGAAACAAGTAGCGGCTTGCTTGTTGAAATATGTGAGAATATAATATCAGCATTCAAAAATATGAAGGCAGCAGACAAGCGCATGGAGGTCTTGTCACTTGGAAAGGAAGCACTATTTACGGCAGCTACGATCGTATCAGCAAAGTCACAGTATGTTACAGGTCAGCTGCAAATAGCTATTGGTGAAAGCTTAACAAGAACTGAGGAACGTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000793 GO:0001067 GO:0002215 GO:0002376 GO:0002682 GO:0002683 GO:0002831 GO:0002832 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009627 GO:0009814 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010112 GO:0010113 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016043 GO:0016444 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030915 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031348 GO:0031399 GO:0031400 GO:0032101 GO:0032102 GO:0032268 GO:0032269 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043543 GO:0043900 GO:0043901 GO:0043966 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045087 GO:0045088 GO:0045824 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051276 GO:0051569 GO:0051572 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0097159 GO:0098542 GO:0106068 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1905268 GO:1990234 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

496

Amino Acids

54.87

Weight (kDa)

4.91

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 597
AccII CGCG 1 cut(s) 132
AciI CCGC 2 cut(s) 762, 1284
AclWI GGATC 1 cut(s) 52
AcsI RAATTY 1 cut(s) 770
AfaI GTAC 5 cut(s) 19, 147, 959, 1034, 1156
AfiI CCNNNNNNNGG 3 cut(s) 155, 204, 597
AjnI CCWGG 1 cut(s) 987
Alw26I GTCTC 2 cut(s) 222, 1245
AlwI GGATC 1 cut(s) 52
Ama87I CYCGRG 2 cut(s) 800, 903
ApeKI GCWGC 8 cut(s) 134, 779, 782, 785, 1225, 1340, 1394, 1438
ApoI RAATTY 1 cut(s) 770
AseI ATTAAT 2 cut(s) 332, 540
Asp700I GAANNNNTTC 3 cut(s) 126, 240, 1239
AspLEI GCGC 3 cut(s) 134, 1148, 1353
AspS9I GGNCC 2 cut(s) 416, 1085
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 3 cut(s) 712, 1132, 1466
AvaI CYCGRG 2 cut(s) 800, 903
AvaII GGWCC 2 cut(s) 416, 1085
BaeGI GKGCMC 1 cut(s) 327
BaeI ACNNNNGTAYC 2 cut(s) 1389, 1422
BbvI GCAGC 8 cut(s) 121, 766, 769, 772, 1212, 1352, 1406, 1425
BccI CCATC 3 cut(s) 206, 905, 1262
BceAI ACGGC 2 cut(s) 110, 1407
BciT130I CCWGG 1 cut(s) 989
BciVI GTATCC 1 cut(s) 366
BcnI CCSGG 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 222, 1245
BfaI CTAG 3 cut(s) 221, 381, 842
BfuI GTATCC 1 cut(s) 366
BglII AGATCT 1 cut(s) 920
BisI GCNGC 9 cut(s) 135, 780, 783, 786, 1226, 1285, 1341, 1395, 1439
BlsI GCNGC 9 cut(s) 136, 781, 784, 787, 1227, 1286, 1342, 1396, 1440
BmcAI AGTACT 1 cut(s) 1034
Bme1390I CCNGG 2 cut(s) 12, 989
Bme18I GGWCC 2 cut(s) 416, 1085
BmeT110I CYCGRG 2 cut(s) 800, 903
BmgT120I GGNCC 2 cut(s) 416, 1085
BmiI GGNNCC 1 cut(s) 417
BmrFI CCNGG 2 cut(s) 12, 989
BmrI ACTGGG 1 cut(s) 607
BmsI GCATC 6 cut(s) 82, 454, 500, 766, 1090, 1112
BmuI ACTGGG 1 cut(s) 607
BpmI CTGGAG 3 cut(s) 965, 971, 1074
BpuEI CTTGAG 2 cut(s) 223, 1037
BpuMI CCSGG 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 231, 275, 889
BsaXI ACNNNNNCTCC 2 cut(s) 1349, 1379
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 204, 597
Bse1I ACTGG 3 cut(s) 315, 419, 602
BseBI CCWGG 1 cut(s) 989
BseDI CCNNGG 3 cut(s) 231, 275, 889
BseGI GGATG 2 cut(s) 97, 311
BseLI CCNNNNNNNGG 3 cut(s) 155, 204, 597
BseMII CTCAG 2 cut(s) 599, 1464
BseNI ACTGG 3 cut(s) 315, 419, 602
BseRI GAGGAG 2 cut(s) 418, 915
BseSI GKGCMC 1 cut(s) 327
BseXI GCAGC 8 cut(s) 121, 766, 769, 772, 1212, 1352, 1406, 1425
BsgI GTGCAG 2 cut(s) 900, 1205
Bsh1236I CGCG 1 cut(s) 132
Bsh1285I CGRYCG 1 cut(s) 1404
BsiEI CGRYCG 1 cut(s) 1404
BsiHKCI CYCGRG 2 cut(s) 800, 903
BsiSI CCGG 1 cut(s) 12
BslFI GGGAC 2 cut(s) 429, 569
BslI CCNNNNNNNGG 3 cut(s) 155, 204, 597
BsmAI GTCTC 2 cut(s) 222, 1245
BsmFI GGGAC 2 cut(s) 429, 569
BsmI GAATGC 1 cut(s) 1322
BsoBI CYCGRG 2 cut(s) 800, 903
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 4 cut(s) 57, 727, 920, 1401
Bsp19I CCATGG 1 cut(s) 231
BspACI CCGC 2 cut(s) 762, 1284
BspCNI CTCAG 2 cut(s) 600, 1465
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 417
BspPI GGATC 1 cut(s) 52
BsrI ACTGG 3 cut(s) 315, 419, 602
BssECI CCNNGG 3 cut(s) 231, 275, 889
BssMI GATC 4 cut(s) 57, 727, 920, 1401
BssT1I CCWWGG 2 cut(s) 231, 889
Bst2UI CCWGG 1 cut(s) 989
Bst4CI ACNGT 2 cut(s) 575, 1422
Bst6I CTCTTC 2 cut(s) 36, 339
BstC8I GCNNGC 2 cut(s) 323, 1289
BstDEI CTNAG 2 cut(s) 608, 1473
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 2 cut(s) 97, 311
BstFNI CGCG 1 cut(s) 132
BstHHI GCGC 3 cut(s) 134, 1148, 1353
BstKTI GATC 4 cut(s) 60, 730, 923, 1404
BstMAI GTCTC 2 cut(s) 222, 1245
BstMBI GATC 4 cut(s) 57, 727, 920, 1401
BstMCI CGRYCG 1 cut(s) 1404
BstMWI GCNNNNNNNGC 4 cut(s) 131, 761, 785, 791
BstNI CCWGG 1 cut(s) 989
BstNSI RCATGY 2 cut(s) 520, 631
BstSCI CCNGG 2 cut(s) 10, 987
BstSLI GKGCMC 1 cut(s) 327
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 8 cut(s) 121, 766, 769, 772, 1212, 1352, 1406, 1425
BstX2I RGATCY 1 cut(s) 920
BstYI RGATCY 1 cut(s) 920
BsuI GTATCC 1 cut(s) 366
BtgI CCRYGG 1 cut(s) 231
BtsCI GGATG 2 cut(s) 97, 311
Cac8I GCNNGC 2 cut(s) 323, 1289
CfoI GCGC 3 cut(s) 134, 1148, 1353
Cfr13I GGNCC 2 cut(s) 416, 1085
Csp6I GTAC 5 cut(s) 18, 146, 958, 1033, 1155
CviQI GTAC 5 cut(s) 18, 146, 958, 1033, 1155
DdeI CTNAG 2 cut(s) 608, 1473
DpnI GATC 4 cut(s) 59, 729, 922, 1403
DpnII GATC 4 cut(s) 57, 727, 920, 1401
Eam1104I CTCTTC 2 cut(s) 36, 339
EarI CTCTTC 2 cut(s) 36, 339
Eco130I CCWWGG 2 cut(s) 231, 889
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 2 cut(s) 416, 1085
Eco88I CYCGRG 2 cut(s) 800, 903
EcoRII CCWGG 1 cut(s) 987
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 2 cut(s) 231, 889
EcoT22I ATGCAT 1 cut(s) 1105
ErhI CCWWGG 2 cut(s) 231, 889
FalI AAGNNNNNCTT 4 cut(s) 662, 694, 957, 989
FaqI GGGAC 2 cut(s) 429, 569
FauI CCCGC 1 cut(s) 769
Fnu4HI GCNGC 9 cut(s) 135, 780, 783, 786, 1226, 1285, 1341, 1395, 1439
FokI GGATG 2 cut(s) 104, 298
Fsp4HI GCNGC 9 cut(s) 135, 780, 783, 786, 1226, 1285, 1341, 1395, 1439
FspBI CTAG 3 cut(s) 221, 381, 842
GlaI GCGC 3 cut(s) 133, 1147, 1352
GluI GCNGC 9 cut(s) 135, 780, 783, 786, 1226, 1285, 1341, 1395, 1439
GsuI CTGGAG 3 cut(s) 965, 971, 1074
HapII CCGG 1 cut(s) 12
HhaI GCGC 3 cut(s) 134, 1148, 1353
Hin6I GCGC 3 cut(s) 132, 1146, 1351
HinP1I GCGC 3 cut(s) 132, 1146, 1351
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 3 cut(s) 661, 1013, 1459
HinfI GANTC 6 cut(s) 36, 65, 108, 425, 614, 717
HpaII CCGG 1 cut(s) 12
HphI GGTGA 3 cut(s) 712, 1132, 1466
Hpy166II GTNNAC 2 cut(s) 424, 652
Hpy188I TCNGA 9 cut(s) 73, 279, 292, 373, 496, 609, 732, 920, 1267
Hpy188III TCNNGA 8 cut(s) 89, 119, 202, 221, 802, 944, 974, 1091
Hpy8I GTNNAC 2 cut(s) 424, 652
HpyAV CCTTC 5 cut(s) 370, 664, 1068, 1250, 1330
HpyCH4III ACNGT 2 cut(s) 575, 1422
HpyCH4IV ACGT 3 cut(s) 27, 956, 1480
HpyCH4V TGCA 8 cut(s) 467, 788, 822, 832, 881, 1103, 1186, 1441
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 761, 785, 791
HpyF3I CTNAG 2 cut(s) 608, 1473
HpySE526I ACGT 3 cut(s) 27, 956, 1480
HspAI GCGC 3 cut(s) 132, 1146, 1351
Kzo9I GATC 4 cut(s) 57, 727, 920, 1401
LmnI GCTCC 3 cut(s) 395, 856, 1115
Lsp1109I GCAGC 8 cut(s) 121, 766, 769, 772, 1212, 1352, 1406, 1425
LweI GCATC 6 cut(s) 82, 454, 500, 766, 1090, 1112
MaeI CTAG 3 cut(s) 221, 381, 842
MaeII ACGT 3 cut(s) 27, 956, 1480
MaeIII GTNAC 5 cut(s) 569, 692, 1365, 1416, 1426
MalI GATC 4 cut(s) 59, 729, 922, 1403
MboI GATC 4 cut(s) 57, 727, 920, 1401
MboII GAAGA 6 cut(s) 53, 60, 184, 279, 356, 1177
MfeI CAATTG 1 cut(s) 789
MflI RGATCY 1 cut(s) 920
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 8 cut(s) 224, 301, 329, 537, 770, 789, 846, 939
MlyI GAGTC 2 cut(s) 419, 623
MmeI TCCRAC 3 cut(s) 463, 912, 1228
Mph1103I ATGCAT 1 cut(s) 1105
MroXI GAANNNNTTC 3 cut(s) 126, 240, 1239
MseI TTAA 5 cut(s) 332, 540, 620, 1029, 1463
MslI CAYNNNNRTG 1 cut(s) 827
MspA1I CMGCKG 2 cut(s) 764, 1438
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 2 cut(s) 12, 989
MunI CAATTG 1 cut(s) 789
Mva1269I GAATGC 1 cut(s) 1322
MvaI CCWGG 1 cut(s) 989
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 4 cut(s) 131, 761, 785, 791
NciI CCSGG 1 cut(s) 12
NcoI CCATGG 1 cut(s) 231
NdeII GATC 4 cut(s) 57, 727, 920, 1401
NlaIV GGNNCC 1 cut(s) 417
NmuCI GTSAC 2 cut(s) 1365, 1416
NsiI ATGCAT 1 cut(s) 1105
NspI RCATGY 2 cut(s) 520, 631
PaeR7I CTCGAG 1 cut(s) 800
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 369, 1397
PctI GAATGC 1 cut(s) 1322
PdmI GAANNNNTTC 3 cut(s) 126, 240, 1239
PfeI GAWTC 4 cut(s) 36, 65, 108, 717
PflMI CCANNNNNTGG 1 cut(s) 597
PkrI GCNGC 9 cut(s) 136, 781, 784, 787, 1227, 1286, 1342, 1396, 1440
Ple19I CGATCG 1 cut(s) 1404
PleI GAGTC 2 cut(s) 419, 622
PpsI GAGTC 2 cut(s) 419, 622
PshBI ATTAAT 2 cut(s) 332, 540
Psp6I CCWGG 1 cut(s) 987
PspGI CCWGG 1 cut(s) 987
PspN4I GGNNCC 1 cut(s) 417
PspPI GGNCC 2 cut(s) 416, 1085
PsrI GAACNNNNNNTAC 2 cut(s) 151, 183
PsuI RGATCY 1 cut(s) 920
PvuI CGATCG 1 cut(s) 1404
PvuII CAGCTG 1 cut(s) 1438
RsaI GTAC 5 cut(s) 19, 147, 959, 1034, 1156
RsaNI GTAC 5 cut(s) 18, 146, 958, 1033, 1155
RseI CAYNNNNRTG 1 cut(s) 827
SaqAI TTAA 5 cut(s) 332, 540, 620, 1029, 1463
SatI GCNGC 9 cut(s) 135, 780, 783, 786, 1226, 1285, 1341, 1395, 1439
Sau3AI GATC 4 cut(s) 57, 727, 920, 1401
Sau96I GGNCC 2 cut(s) 416, 1085
ScaI AGTACT 1 cut(s) 1034
SchI GAGTC 2 cut(s) 419, 623
ScrFI CCNGG 2 cut(s) 12, 989
SduI GDGCHC 1 cut(s) 327
SfaNI GCATC 6 cut(s) 82, 454, 500, 766, 1090, 1112
Sfr274I CTCGAG 1 cut(s) 800
SinI GGWCC 2 cut(s) 416, 1085
SlaI CTCGAG 1 cut(s) 800
SmiMI CAYNNNNRTG 1 cut(s) 827
SmlI CTYRAG 3 cut(s) 202, 800, 1016
SmoI CTYRAG 3 cut(s) 202, 800, 1016
Sse9I AATT 8 cut(s) 224, 301, 329, 537, 770, 789, 846, 939
SsiI CCGC 2 cut(s) 762, 1284
SspMI CTAG 3 cut(s) 221, 381, 842
StyD4I CCNGG 2 cut(s) 10, 987
StyI CCWWGG 2 cut(s) 231, 889
TaaI ACNGT 2 cut(s) 575, 1422
TaiI ACGT 3 cut(s) 30, 959, 1483
TaqI TCGA 4 cut(s) 39, 63, 801, 996
TasI AATT 8 cut(s) 224, 301, 329, 537, 770, 789, 846, 939
TatI WGTACW 1 cut(s) 1032
TauI GCSGC 1 cut(s) 1287
TfiI GAWTC 4 cut(s) 36, 65, 108, 717
Tru1I TTAA 5 cut(s) 332, 540, 620, 1029, 1463
Tru9I TTAA 5 cut(s) 332, 540, 620, 1029, 1463
TseFI GTSAC 2 cut(s) 1365, 1416
TseI GCWGC 8 cut(s) 134, 779, 782, 785, 1225, 1340, 1394, 1438
Tsp45I GTSAC 2 cut(s) 1365, 1416
TspDTI ATGAA 4 cut(s) 489, 608, 1014, 1349
TspGWI ACGGA 2 cut(s) 171, 1128
Van91I CCANNNNNTGG 1 cut(s) 597
VpaK11BI GGWCC 2 cut(s) 416, 1085
VspI ATTAAT 2 cut(s) 332, 540
XapI RAATTY 1 cut(s) 770
XbaI TCTAGA 1 cut(s) 220
XceI RCATGY 2 cut(s) 520, 631
XhoI CTCGAG 1 cut(s) 800
XmnI GAANNNNTTC 3 cut(s) 126, 240, 1239
XspI CTAG 3 cut(s) 221, 381, 842
ZrmI AGTACT 1 cut(s) 1034
Zsp2I ATGCAT 1 cut(s) 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.