Rh5BG072500

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001798
Physical Location & Seq
Forward (+)
5965535 .. 5971586
6052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG072500.1

Sequence Viewer

Length: 1128 bp
ATGGAGAACCCTAGCAGTAAGAGAACCACCGCCGGAGCTCTGGAAGAGAACATCTTGGCCATCCTCGACTCCACCGAAGCCGACGACACTCACTACGCCAACGACGACCGAACTGCTTTCCTCGACGCCGTTCGTGCCGCTTCGCTTGTACCCGAAAATGGAACTCCACCTACCTATAAATGGTTTGAGTCGATCTTCGCCATCTTGAGGATTGGGAAATCTCTGGAGCTGACTATGGCAAGTTTTAAGCTTTTGAATGAGTTGGATAAGCGTTTCCCTCGGGTGCATTTGTCTGTTGTTGATGAAATGAAATCGTCCAATAAAGTCATGTTCAATGAGGCTTGGTCGCCATTTGTTTTCAGCTCCGAAAGTACTAGTAGCGAGAGGGAAGCTGCTAATAACTTGGGGGGATCAGTTGACTCCTCTGGTTTCCAACATTTGATAGAAGACCTTGCTGATGCAACTGCTGAACCAAACTTCCAAGCCTCAGAAACAAAGTCCCTAGGAAACATGTTGCTGTTTCAATACCTAGTCAACGTTCTTGAAGGAGATTTTCAACCTCGCAACTGTGTCTACGCAGAAACTGCAAACTGGGTCCTTCTGAGAGAGTCTTTGCTCAACATGCTTTTGGTATCAAGAAAGATAAACTACAAAAACTTGATGAAGGATTGCTTGGCCATTATGTGTAAGCTGTATCAAACCAATGCTGGATTTGCTGATGATCTAATAAGTTCAGAGACGTCTGTGGCACAACCAGCTGAAAATTTTGATACGGCTGCAACAATTGCTTTACTTGAGATAGGAAAGAATACCTGTATAGCCATGCAGAAGTTTCTGATAATGATTATGGAGCTTGACTTGTCGAAGAAGACAGCAGAAATGAAAGGGTCTACTACAAGAGCAGATGGTGTCAGAACTCCTCTGCTGGAGATAGTTCTGGATGAGCTAACTTACGATAAGGATATTCTTGACTCATTTCTTCAGGTTTTCAATGAACCTAAATGGAAGCTTGAGATAGTTGTGCAGTATTTGTGGAAATACATTGGTAAGGTGAGCTATCAGAGTTATAGAAAACCAGTTATGTGTCCAAATCAGCCAACAAAGGTCCTTCTGCTTGTATTGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000793 GO:0001067 GO:0002215 GO:0002376 GO:0002682 GO:0002683 GO:0002831 GO:0002832 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009627 GO:0009814 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010112 GO:0010113 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010639 GO:0016043 GO:0016444 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030915 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031348 GO:0031399 GO:0031400 GO:0032101 GO:0032102 GO:0032268 GO:0032269 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043543 GO:0043900 GO:0043901 GO:0043966 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045087 GO:0045088 GO:0045824 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051276 GO:0051569 GO:0051572 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0097159 GO:0098542 GO:0106068 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1905268 GO:1990234 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

375

Amino Acids

42.1

Weight (kDa)

4.89

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 743
AccI GTMKAC 2 cut(s) 573, 890
AciI CCGC 2 cut(s) 30, 138
AclI AACGTT 1 cut(s) 537
AclWI GGATC 1 cut(s) 418
AcoI YGGCCR 2 cut(s) 57, 675
AcsI RAATTY 1 cut(s) 763
AcuI CTGAAG 1 cut(s) 965
AcyI GRCGYC 2 cut(s) 126, 740
AfaI GTAC 2 cut(s) 150, 373
AfiI CCNNNNNNNGG 3 cut(s) 158, 180, 207
AflIII ACRYGT 1 cut(s) 510
AgsI TTSAA 6 cut(s) 256, 334, 524, 545, 557, 991
AhlI ACTAGT 1 cut(s) 374
AjuI GAANNNNNNNTTGG 2 cut(s) 656, 688
AloI GAACNNNNNNTCC 2 cut(s) 462, 494
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 731
AlwI GGATC 1 cut(s) 418
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 2 cut(s) 57, 675
ApeKI GCWGC 2 cut(s) 392, 776
ApoI RAATTY 1 cut(s) 763
AspA2I CCTAGG 1 cut(s) 502
AspS9I GGNCC 2 cut(s) 595, 1105
AsuHPI GGTGA 1 cut(s) 1063
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 2 cut(s) 595, 1105
AvrII CCTAGG 1 cut(s) 502
BalI TGGCCA 2 cut(s) 59, 677
BanII GRGCYC 1 cut(s) 40
BbsI GAAGAC 2 cut(s) 453, 875
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 2 cut(s) 379, 763
BccI CCATC 3 cut(s) 68, 209, 899
BceAI ACGGC 2 cut(s) 113, 789
BcgI CGANNNNNNTGC 2 cut(s) 95, 129
BcoDI GTCTC 1 cut(s) 731
BcuI ACTAGT 1 cut(s) 374
BfaI CTAG 4 cut(s) 12, 375, 503, 530
BisI GCNGC 3 cut(s) 138, 393, 777
BlnI CCTAGG 1 cut(s) 502
BlsI GCNGC 3 cut(s) 139, 394, 778
BmcAI AGTACT 1 cut(s) 373
Bme18I GGWCC 2 cut(s) 595, 1105
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 2 cut(s) 595, 1105
BmiI GGNNCC 1 cut(s) 596
BmrI ACTGGG 1 cut(s) 601
BmsI GCATC 1 cut(s) 448
BmuI ACTGGG 1 cut(s) 601
BpiI GAAGAC 2 cut(s) 453, 875
BpmI CTGGAG 2 cut(s) 245, 947
BpuEI CTTGAG 3 cut(s) 226, 815, 1031
BsaHI GRCGYC 2 cut(s) 126, 740
BsaJI CCNNGG 2 cut(s) 278, 502
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 180, 207
Bse1I ACTGG 2 cut(s) 596, 1076
BseDI CCNNGG 2 cut(s) 278, 502
BseGI GGATG 2 cut(s) 60, 946
BseLI CCNNNNNNNGG 3 cut(s) 158, 180, 207
BseMII CTCAG 2 cut(s) 501, 593
BseNI ACTGG 2 cut(s) 596, 1076
BseRI GAGGAG 2 cut(s) 412, 909
BseXI GCAGC 2 cut(s) 379, 763
BsgI GTGCAG 1 cut(s) 1043
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 2 cut(s) 59, 677
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 40
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 3 cut(s) 158, 180, 207
BsmAI GTCTC 1 cut(s) 731
BsmBI CGTCTC 1 cut(s) 731
BsmFI GGGAC 1 cut(s) 484
BsnI GGCC 2 cut(s) 59, 677
BsoBI CYCGRG 1 cut(s) 279
Bsp1286I GDGCHC 1 cut(s) 40
Bsp143I GATC 3 cut(s) 192, 410, 721
BspACI CCGC 2 cut(s) 30, 138
BspANI GGCC 2 cut(s) 59, 677
BspCNI CTCAG 2 cut(s) 500, 594
BspHI TCATGA 1 cut(s) 1124
BspLI GGNNCC 1 cut(s) 596
BspPI GGATC 1 cut(s) 418
BsrI ACTGG 2 cut(s) 596, 1076
BssECI CCNNGG 2 cut(s) 278, 502
BssMI GATC 3 cut(s) 192, 410, 721
BssNI GRCGYC 2 cut(s) 126, 740
BssT1I CCWWGG 1 cut(s) 502
Bst4CI ACNGT 1 cut(s) 569
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 2 cut(s) 126, 740
BstAPI GCANNNNNTGC 2 cut(s) 584, 785
BstDEI CTNAG 2 cut(s) 487, 602
BstF5I GGATG 2 cut(s) 60, 946
BstKTI GATC 3 cut(s) 195, 413, 724
BstMAI GTCTC 1 cut(s) 731
BstMBI GATC 3 cut(s) 192, 410, 721
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 6 cut(s) 134, 584, 622, 713, 755, 785
BstNSI RCATGY 2 cut(s) 514, 625
BstV1I GCAGC 2 cut(s) 379, 763
BstV2I GAAGAC 2 cut(s) 453, 875
BsuRI GGCC 2 cut(s) 59, 677
BtsCI GGATG 2 cut(s) 60, 946
CaiI CAGNNNCTG 1 cut(s) 584
CciI TCATGA 1 cut(s) 1124
Cfr13I GGNCC 2 cut(s) 595, 1105
CseI GACGC 1 cut(s) 134
Csp6I GTAC 2 cut(s) 149, 372
CviAII CATG 5 cut(s) 328, 511, 622, 823, 1125
CviQI GTAC 2 cut(s) 149, 372
DdeI CTNAG 2 cut(s) 487, 602
DpnI GATC 3 cut(s) 194, 412, 723
DpnII GATC 3 cut(s) 192, 410, 721
EaeI YGGCCR 2 cut(s) 57, 675
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Ecl136II GAGCTC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 502
Eco24I GRGCYC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 595, 1105
Eco53kI GAGCTC 1 cut(s) 38
Eco57I CTGAAG 1 cut(s) 965
Eco88I CYCGRG 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 38
EcoO109I RGGNCCY 2 cut(s) 595, 1105
EcoT14I CCWWGG 1 cut(s) 502
EcoT38I GRGCYC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 502
Esp3I CGTCTC 1 cut(s) 731
FaeI CATG 5 cut(s) 331, 514, 625, 826, 1128
FalI AAGNNNNNCTT 2 cut(s) 656, 688
FaqI GGGAC 1 cut(s) 484
FatI CATG 5 cut(s) 327, 510, 621, 822, 1124
FblI GTMKAC 2 cut(s) 573, 890
Fnu4HI GCNGC 3 cut(s) 138, 393, 777
FokI GGATG 2 cut(s) 47, 953
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 3 cut(s) 138, 393, 777
FspBI CTAG 4 cut(s) 12, 375, 503, 530
GluI GCNGC 3 cut(s) 138, 393, 777
GsuI CTGGAG 2 cut(s) 245, 947
HaeIII GGCC 2 cut(s) 59, 677
HapII CCGG 1 cut(s) 33
HgaI GACGC 1 cut(s) 134
Hin1I GRCGYC 2 cut(s) 126, 740
Hin1II CATG 5 cut(s) 331, 514, 625, 826, 1128
HincII GTYRAC 2 cut(s) 418, 535
HindII GTYRAC 2 cut(s) 418, 535
HindIII AAGCTT 2 cut(s) 248, 1007
HinfI GANTC 5 cut(s) 68, 188, 419, 608, 971
HpaII CCGG 1 cut(s) 33
HphI GGTGA 1 cut(s) 1063
Hpy166II GTNNAC 4 cut(s) 418, 535, 574, 891
Hpy188I TCNGA 7 cut(s) 367, 490, 603, 736, 837, 914, 1062
Hpy188III TCNNGA 8 cut(s) 41, 205, 224, 542, 636, 938, 968, 1125
Hpy8I GTNNAC 4 cut(s) 418, 535, 574, 891
Hpy99I CGWCG 3 cut(s) 86, 107, 128
HpyAV CCTTC 4 cut(s) 539, 608, 658, 1118
HpyCH4III ACNGT 1 cut(s) 569
HpyCH4IV ACGT 2 cut(s) 537, 740
HpyCH4V TGCA 6 cut(s) 286, 461, 587, 779, 826, 1024
HpyF10VI GCNNNNNNNGC 6 cut(s) 134, 584, 622, 713, 755, 785
HpyF3I CTNAG 2 cut(s) 487, 602
HpySE526I ACGT 2 cut(s) 537, 740
Hsp92I GRCGYC 2 cut(s) 126, 740
Hsp92II CATG 5 cut(s) 331, 514, 625, 826, 1128
Kzo9I GATC 3 cut(s) 192, 410, 721
LmnI GCTCC 4 cut(s) 35, 226, 368, 850
Lsp1109I GCAGC 2 cut(s) 379, 763
LweI GCATC 1 cut(s) 448
MaeI CTAG 4 cut(s) 12, 375, 503, 530
MaeII ACGT 2 cut(s) 537, 740
MalI GATC 3 cut(s) 194, 412, 723
MboI GATC 3 cut(s) 192, 410, 721
MboII GAAGA 6 cut(s) 56, 187, 458, 877, 880, 971
MfeI CAATTG 1 cut(s) 783
MhlI GDGCHC 1 cut(s) 40
MlsI TGGCCA 2 cut(s) 59, 677
MluCI AATT 2 cut(s) 763, 783
MluNI TGGCCA 2 cut(s) 59, 677
MlyI GAGTC 5 cut(s) 62, 197, 413, 617, 965
MmeI TCCRAC 2 cut(s) 243, 457
Mox20I TGGCCA 2 cut(s) 59, 677
MscI TGGCCA 2 cut(s) 59, 677
MseI TTAA 1 cut(s) 246
Msp20I TGGCCA 2 cut(s) 59, 677
MspA1I CMGCKG 1 cut(s) 758
MspI CCGG 1 cut(s) 33
MunI CAATTG 1 cut(s) 783
MwoI GCNNNNNNNGC 6 cut(s) 134, 584, 622, 713, 755, 785
NdeII GATC 3 cut(s) 192, 410, 721
NlaIII CATG 5 cut(s) 331, 514, 625, 826, 1128
NlaIV GGNNCC 1 cut(s) 596
NspI RCATGY 2 cut(s) 514, 625
PagI TCATGA 1 cut(s) 1124
PciI ACATGT 1 cut(s) 510
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 102
PkrI GCNGC 3 cut(s) 139, 394, 778
PleI GAGTC 5 cut(s) 62, 196, 413, 616, 965
PpsI GAGTC 5 cut(s) 62, 196, 413, 616, 965
PpuMI RGGWCCY 2 cut(s) 595, 1105
PscI ACATGT 1 cut(s) 510
Psp124BI GAGCTC 1 cut(s) 40
Psp1406I AACGTT 1 cut(s) 537
Psp5II RGGWCCY 2 cut(s) 595, 1105
PspN4I GGNNCC 1 cut(s) 596
PspPI GGNCC 2 cut(s) 595, 1105
PspPPI RGGWCCY 2 cut(s) 595, 1105
PsrI GAACNNNNNNTAC 2 cut(s) 154, 186
PstNI CAGNNNCTG 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 758
RsaI GTAC 2 cut(s) 150, 373
RsaNI GTAC 2 cut(s) 149, 372
SacI GAGCTC 1 cut(s) 40
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 3 cut(s) 138, 393, 777
Sau3AI GATC 3 cut(s) 192, 410, 721
Sau96I GGNCC 2 cut(s) 595, 1105
ScaI AGTACT 1 cut(s) 373
SchI GAGTC 5 cut(s) 62, 197, 413, 617, 965
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 1 cut(s) 448
SinI GGWCC 2 cut(s) 595, 1105
SmlI CTYRAG 3 cut(s) 205, 794, 1010
SmoI CTYRAG 3 cut(s) 205, 794, 1010
SpeI ACTAGT 1 cut(s) 374
Sse9I AATT 2 cut(s) 763, 783
SsiI CCGC 2 cut(s) 30, 138
SspMI CTAG 4 cut(s) 12, 375, 503, 530
SstI GAGCTC 1 cut(s) 40
StyI CCWWGG 1 cut(s) 502
TaaI ACNGT 1 cut(s) 569
TaiI ACGT 2 cut(s) 540, 743
TaqI TCGA 4 cut(s) 66, 123, 191, 863
TaqII GACCGA 1 cut(s) 123
TasI AATT 2 cut(s) 763, 783
TatI WGTACW 1 cut(s) 371
TauI GCSGC 1 cut(s) 140
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TseI GCWGC 2 cut(s) 392, 776
TspDTI ATGAA 5 cut(s) 318, 323, 677, 896, 1008
VpaK11BI GGWCC 2 cut(s) 595, 1105
XapI RAATTY 1 cut(s) 763
XceI RCATGY 2 cut(s) 514, 625
XmaJI CCTAGG 1 cut(s) 502
XmiI GTMKAC 2 cut(s) 573, 890
XspI CTAG 4 cut(s) 12, 375, 503, 530
ZraI GACGTC 1 cut(s) 741
ZrmI AGTACT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.