Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001052

Physical Location & Seq

Genomic Coordinates Forward (+)

19923353 .. 19926388

Gene Structure 3036 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1081100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3036 bp

ATGATGAACAATCTTAAGCTCGGGGTAGATGTAGTTGGCGCGCACAATCTTTTGCCTAAAGATGGGCAAGGTTCATCCAATGCTTTTGTGGAGCTTTACTTTGATGGTCAAAGGTTCCGCACCACTATCAAAGAAAAGGATCTAAACCCCGTTTGGAACGAGAGCTTCTATTTCAACATTTCTGATCCTTCAAACCTGCATTATCTTACACTAGAGGCCTATGTCTACAATAATGTCAAAGCTAGCTACTCCAGGTCCTTCCTTGGAAAGATTAGTCTCACCGGAAACTCATTTGTCCCCTATTCAGATGCTGTTGTCTTGCATTACCCTTTGGAAAAGCGCGGCATCTTTTCCCGTGTAAGAGGAGAGCTTGGCCTCAAGGTGTATGTTACTGATGACACGTCAATCAGGTCTTCTACTCCAGTCCCTAATGCCGAATCCCTCGCAAATGCGGATCCAAGTGCAGCTCATGGACACACCGAAGGAGTTCCAAATCCTGTCATGAACGCTTTGCGAAATGAGAGAGCTGGGACCAGACACAGCTTTCATCATCTTCCTCATCCAAGTCATGACGAACAACATCAGCATCGTGCTTCTTCTGCACCGGATGAGCATTATGTGCCCAAGTATGAAGCTGATCAAATGAAACAGGAACAACCGCAGCCTGCAAAGCTGGTTCGCATGCACTCTGCTGCATCATCACAACCGGTTGATTTTGCACTAAAAGAGACTAGCCCTTACCTTGGAGGTGGAAGAGTTGTTGGTGGCCGTGTTATTCACGGAGACAAGACTGCAAGCACCTATGATCTCGTGGAACGCATGTATTTTTTATACGTAAGGGTTGTCAAGGCTCGTGAGCTTCCTGCCATGGATGTCACAGGGAGTCTTGATCCGTATGTTGAAGTCAGAATTGGAAACTACAGAGGGATTACAAAGCACTTTGAGAAGCAGCAAAATCCTACATGGAATCAGGTTTTTGCCTTCTCAAAGGATCGAATGCAAGCATCTGTATTAGAAGTTGTGATTAAGGACAAGGATCTAATTAAAGATGATTTTGTGGGGCTTGTAAGATTCGACATCAATGAGGTGCCGATGAGAGTCCCGCCAGATAGTCCTCTGGCTCCAGAATGGTACCGGCTTGAGGACAAGAAGGGAGAAAAGATTAAGAGTGAACTGATGCTCGCAGTCTGGATTGGAACGCAAGCAGATGAGGCCTTTTCTGATGCATGGCATTCTGATGCAGCTACTCCTGTTGATAGCACACCAGCTGCCTCCACACTAATACGTTCAAAGGTCTATCATGCACCGAGGTTGTGGTATGTGCGTGTCAATGTTATCGAGGCACAAGACCTTTTTGCAGCAGAGAAGAATCGCTTTCCGGAATCATATGTCAAGGTACAGCTTGGAAACCAGGTTTTGAAAACGAAGACACTTCAGGCTCGGAATCTAAACCCACTTTGGAATGAAGATCTTTTGTTTGTTGCTTCTGAACCCTTTGAAGATCATCTTATTATTTCAGTTGAGGATCGTGTGGGCCCTGGAAGAGATGAAATCATTGGGAGGGTCATATTACCGCTGAATGCTGTAGATAGGCGCGCTGATGATCGTATGATCCATTCCCGATGGTTTAATCTGGAAAAGCCAGTTGCTGTTGACATAGATCAGTTGAAAAGGGACAAGTTCTCAAGCCGCCTTCATCTTCGAGTTTGTCTTGATGGAGGTTATCATGTTCTTGATGAATCAACTCATTACAGTAGTGATTTACGCCCTACTGCAAAGCAGCTCTGGAGGCCATCAATTGGGGTGCTAGAGCTCGGAATCTTGAATGCATCAGGGCTTCACCCAATGAAAACACGCAATGAAAAGGGCACATCAGATACTTACTGTGTGGCAAAGTATGGTCATAAATGGGTCCGGACAAGAACCCTTGTTGATAACCTGAGTCCAAAATACAATGAGCAGTACACTTGGGAAGTATTTGACCCTGCTACAGTTCTTACTGTAGGTGTATTCGACAACAGTCAGCTCGGGGATAAGGATTCCCATGGTAACAAGGACCTGAAGATTGGGAAGGTTCGTGTTCGTATCTCAACACTTGAAGCAGGACGTATATACACACACTCTTATCCCCTGCTGGTTCTTCACCCAACTGGTGTTAAGAAGATGGGGGAGCTGCATTTGGCAATTCGGTTTTCATGCACTTCTTTTGTAAACATGATGTTCGTATACTCAAAACCACTGCTGCCAAAGATGCATTATGTGAGGCCCTTCAACATTATGCAGCTTGACATGTTACGTCACCAAGCTGTCAACATAGTTGCAGCAAGGCTTGGCCGAGCGGAGCCACCACTTAGGAAGGAAGTGGTGGAATACATGTCTGATGTGGACTCGCATCTTTGGAGTATGAGGCGGAGCAAAGCGAATTTCTTTAGGCTAATGACGGTTTTCTCAGGAGTATTTGCTGTAGGCAAATGGTTTACTGATATATGCATGTGGAAGAATCCTATCACAACAGTGCTTGTTCATGTTCTCTTCCTTATGCTTGTTTGCTTCCCAGAATTAATTCTGCCCACAGTGTTTCTTTACATGTTTCTGATAGGGATATGGAACTTCCGCTACAGGCCCAGATATCCTCCCCACATGAACACAAAGATCTCACAAGCCGAGTTAGTGCACCCCGATGAGCTAGATGAGGAGTTCGACACATTCCCAACAAGCAAGAACCCAGAGCTGGTGAGAATGAGGTATGATCGCCTAAGGAGCGTAGCTGGTAGAATTCAGACTGTTGTTGGTGATGTAGCTACCCAAGGGGAGCGGTTTCAGGCACTGTTAAGCTGGCGCGATCCACGCGCTTCAGCACTTTTTGTCACATTCTGCCTCATAGCTGCCTTGGTGATGTACGTTACACCGTTCCAGGTAGTGGCAGCATTGGCAGGGTTCTTTATGATGAGGCATCCGAGATTTCGCAATAGGCTACCATCCGCACCCATCAATTTCTTCCGCAGGCTGCCTTCGAGGACCGACAGTATGTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0007154 GO:0007165 GO:0008021 GO:0008150 GO:0009987 GO:0010646 GO:0012505 GO:0012506 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030100 GO:0030133 GO:0030334 GO:0030336 GO:0030658 GO:0030659 GO:0030672 GO:0031090 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032879 GO:0035556 GO:0040012 GO:0040013 GO:0042175 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044456 GO:0044464 GO:0045202 GO:0045806 GO:0046872 GO:0046928 GO:0048167 GO:0048168 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0051046 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051270 GO:0051271 GO:0051588 GO:0051716 GO:0055037 GO:0060341 GO:0060627 GO:0065007 GO:0065008 GO:0070382 GO:0080134 GO:0097458 GO:0097708 GO:0098588 GO:0098793 GO:0098805 GO:0098827 GO:0099177 GO:0099501 GO:0099503 GO:1902882 GO:1902883 GO:1903530 GO:2000145 GO:2000146

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 PRT_C

Protein Analysis

1012

Amino Acids

115.48

Weight (kDa)

9.04

Isoelectric Point (pI)

39.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	6 - 97	5.4e-18	C2 domain
C2	PF00168	277 - 379	6.4e-20	C2 domain
C2	PF00168	440 - 545	1.4e-20	C2 domain
C2	PF00168	601 - 711	1.3e-18	C2 domain
PRT_C	PF08372	856 - 1011	2.2e-80	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001052)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11610 AT4G11610 AT4G11610
fragaria_vesca	FvH4_5g02110 FvH4_5g02110 FvH4_5g02110 FvH4_5g02110 FvH4_5g02110 FvH4_5g02110 FvH4_5g02110 FvH4_5g02110 FvH4_5g02110 FvH4_5g02110 FvH4_5g02110 FvH4_7g13890 FvH4_7g13890 FvH4_7g13890 FvH4_7g13890 FvH4_7g13890
malus_domestica	MD01G1055400.v1.1 MD06G1081100.v1.1 MD07G1139900.v1.1 MD17G1025600.v1.1
prunus_persica	Prupe.2G165500_v2.0.a1 Prupe.2G165500_v2.0.a1 Prupe.5G098200_v2.0.a1 Prupe.5G098200_v2.0.a1
pyrus_communis	pycom01g08090 pycom06g07810 pycom07g13790 pycom14g09900 pycom17g16030
rosa_chinensis	RchiOBHm_Chr1g0354121 RchiOBHm_Chr1g0354261 RchiOBHm_Chr7g0198021
rosa_laevigata	RLG00000003892 RLG00000028225
rosa_multiflora	Rmu_co8337093.1_g000001 Rmu_co8489259.1_g000001 Rmu_co8505511.1_g000001 Rmu_sc0000221.1_g000032 Rmu_sc0001926.1_g000016 Rmu_sc0006991.1_g000004
rosa_roxburghii	Rroxscaffold_3G00258010 Rroxscaffold_4G00301210
rosa_rugosa	Rorug01G0241600 Rorug01G0241700 Rorug07G0045400 Rorug07G0045500
rosa_samantha	Rh1AG252300 Rh1BG224200 Rh1CG236900 Rh1DG251200 Rh7AG168800 Rh7BG171900 Rh7CG178400 Rh7DG171200
rosa_wichuraiana	Rw1G022010 Rw1G022130 Rw7G014760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	204
Acc65I	GGTACC	1 cut(s)	1131
AccB1I	GGYRCC	2 cut(s)	1087, 1131
AccB7I	CCANNNNNTGG	2 cut(s)	1799, 2920
AccBSI	CCGCTC	2 cut(s)	2339, 2815
AccI	GTMKAC	2 cut(s)	225, 2226
AccII	CGCG	5 cut(s)	41, 342, 1596, 2841, 2850
AccIII	TCCGGA	2 cut(s)	1378, 1914
AcoI	YGGCCR	2 cut(s)	766, 2332
AcsI	RAATTY	2 cut(s)	2422, 2775
AcuI	CTGAAG	3 cut(s)	1418, 2081, 2838
AfaI	GTAC	4 cut(s)	1133, 1398, 1964, 2900
AfiI	CCNNNNNNNGG	6 cut(s)	62, 743, 1141, 1799, 2731, 2920
AflII	CTTAAG	1 cut(s)	14
AflIII	ACRYGT	4 cut(s)	399, 2289, 2373, 2586
AgeI	ACCGGT	1 cut(s)	706
AjiI	CACGTC	1 cut(s)	402
AjnI	CCWGG	4 cut(s)	251, 1410, 1537, 2913
AjuI	GAANNNNNNNTTGG	2 cut(s)	894, 926
AleI	CACNNNNGTG	1 cut(s)	2513
Alw21I	GWGCWC	2 cut(s)	1815, 2676
Alw26I	GTCTC	3 cut(s)	281, 722, 777
Alw44I	GTGCAC	1 cut(s)	2672
AlwNI	CAGNNNCTG	3 cut(s)	311, 1649, 2827
Ama87I	CYCGRG	2 cut(s)	20, 2027
Aor13HI	TCCGGA	2 cut(s)	1378, 1914
AoxI	GGCC	9 cut(s)	216, 373, 766, 1212, 1534, 1790, 2264, 2332, 2621
ApaI	GGGCCC	1 cut(s)	1538
ApaLI	GTGCAC	1 cut(s)	2672
ApoI	RAATTY	2 cut(s)	2422, 2775
AseI	ATTAAT	1 cut(s)	2561
AsiGI	ACCGGT	1 cut(s)	706
Asp700I	GAANNNNTTC	2 cut(s)	486, 2562
Asp718I	GGTACC	1 cut(s)	1131
AspLEI	GCGC	7 cut(s)	41, 43, 342, 1596, 1598, 2841, 2852
AspS9I	GGNCC	9 cut(s)	255, 531, 1534, 1535, 1912, 2056, 2265, 2622, 3018
AsuHPI	GGTGA	7 cut(s)	271, 1832, 2135, 2291, 2746, 2804, 2905
AsuNHI	GCTAGC	1 cut(s)	242
AvaI	CYCGRG	2 cut(s)	20, 2027
AvaII	GGWCC	5 cut(s)	255, 531, 1912, 2056, 3018
AxyI	CCTNAGG	1 cut(s)	2756
BaeGI	GKGCMC	4 cut(s)	624, 1538, 1871, 2676
BamHI	GGATCC	1 cut(s)	454
BanI	GGYRCC	2 cut(s)	1087, 1131
BanII	GRGCYC	2 cut(s)	1538, 1815
BauI	CACGAG	2 cut(s)	809, 852
BbsI	GAAGAC	2 cut(s)	405, 1433
Bbv12I	GWGCWC	2 cut(s)	1815, 2676
BccI	CCATC	8 cut(s)	56, 98, 1617, 1709, 1801, 2158, 2986, 2996
BceAI	ACGGC	1 cut(s)	753
BciT130I	CCWGG	4 cut(s)	253, 1412, 1539, 2915
BclI	TGATCA	1 cut(s)	637
BcoDI	GTCTC	3 cut(s)	281, 722, 777
BfaI	CTAG	5 cut(s)	212, 243, 732, 1808, 2687
BfmI	CTRYAG	6 cut(s)	919, 1584, 1989, 2001, 2463, 2617
BfrI	CTTAAG	1 cut(s)	14
BfuAI	ACCTGC	1 cut(s)	204
BglII	AGATCT	2 cut(s)	1468, 2652
Bme1390I	CCNGG	4 cut(s)	253, 1412, 1539, 2915
Bme18I	GGWCC	5 cut(s)	255, 531, 1912, 2056, 3018
BmeT110I	CYCGRG	2 cut(s)	20, 2027
BmgBI	CACGTC	1 cut(s)	402
BmgT120I	GGNCC	9 cut(s)	255, 531, 1534, 1535, 1912, 2056, 2265, 2622, 3018
BmiI	GGNNCC	9 cut(s)	116, 456, 532, 1089, 1122, 1133, 1536, 1913, 2343
BmrFI	CCNGG	4 cut(s)	253, 1412, 1539, 2915
BmtI	GCTAGC	1 cut(s)	246
BoxI	GACNNNNGTC	1 cut(s)	2019
BpiI	GAAGAC	2 cut(s)	405, 1433
BpmI	CTGGAG	4 cut(s)	235, 405, 1107, 1807
BpuEI	CTTGAG	3 cut(s)	362, 1160, 1669
BsaAI	YACGTR	1 cut(s)	835
BsaJI	CCNNGG	8 cut(s)	262, 742, 867, 1307, 1537, 2044, 2806, 2889
BsaWI	WCCGGW	5 cut(s)	281, 604, 706, 1378, 1914
Bsc4I	CCNNNNNNNGG	6 cut(s)	62, 743, 1141, 1799, 2731, 2920
Bse118I	RCCGGY	2 cut(s)	706, 1134
Bse1I	ACTGG	3 cut(s)	422, 1643, 2155
Bse21I	CCTNAGG	1 cut(s)	2756
Bse3DI	GCAATG	1 cut(s)	1864
BseAI	TCCGGA	2 cut(s)	1378, 1914
BseBI	CCWGG	4 cut(s)	253, 1412, 1539, 2915
BseDI	CCNNGG	8 cut(s)	262, 742, 867, 1307, 1537, 2044, 2806, 2889
BseGI	GGATG	6 cut(s)	74, 559, 613, 877, 2953, 2978
BseLI	CCNNNNNNNGG	6 cut(s)	62, 743, 1141, 1799, 2731, 2920
BseMI	GCAATG	1 cut(s)	1864
BseMII	CTCAG	2 cut(s)	1931, 2463
BseNI	ACTGG	3 cut(s)	422, 1643, 2155
BsePI	GCGCGC	2 cut(s)	39, 1594
BseRI	GAGGAG	2 cut(s)	378, 2708
BseSI	GKGCMC	4 cut(s)	624, 1538, 1871, 2676
BseYI	CCCAGC	1 cut(s)	527
BsgI	GTGCAG	2 cut(s)	483, 585
Bsh1236I	CGCG	5 cut(s)	41, 342, 1596, 2841, 2850
BshFI	GGCC	9 cut(s)	218, 375, 768, 1214, 1536, 1792, 2266, 2334, 2623
BshNI	GGYRCC	2 cut(s)	1087, 1131
BshTI	ACCGGT	1 cut(s)	706
BsiHKAI	GWGCWC	2 cut(s)	1815, 2676
BsiHKCI	CYCGRG	2 cut(s)	20, 2027
BsiSI	CCGG	6 cut(s)	282, 605, 707, 1135, 1379, 1915
BslFI	GGGAC	5 cut(s)	281, 410, 544, 1085, 1688
BslI	CCNNNNNNNGG	6 cut(s)	62, 743, 1141, 1799, 2731, 2920
BsmAI	GTCTC	3 cut(s)	281, 722, 777
BsmFI	GGGAC	5 cut(s)	281, 410, 544, 1085, 1688
BsmI	GAATGC	4 cut(s)	1002, 1231, 1585, 1831
BsnI	GGCC	9 cut(s)	218, 375, 768, 1214, 1536, 1792, 2266, 2334, 2623
BsoBI	CYCGRG	2 cut(s)	20, 2027
Bsp120I	GGGCCC	1 cut(s)	1534
Bsp1286I	GDGCHC	5 cut(s)	624, 1538, 1815, 1871, 2676
Bsp13I	TCCGGA	2 cut(s)	1378, 1914
Bsp19I	CCATGG	2 cut(s)	867, 2044
BspANI	GGCC	9 cut(s)	218, 375, 768, 1214, 1536, 1792, 2266, 2334, 2623
BspCNI	CTCAG	2 cut(s)	1932, 2462
BspEI	TCCGGA	2 cut(s)	1378, 1914
BspFNI	CGCG	5 cut(s)	41, 342, 1596, 2841, 2850
BspHI	TCATGA	2 cut(s)	501, 568
BspLI	GGNNCC	9 cut(s)	116, 456, 532, 1089, 1122, 1133, 1536, 1913, 2343
BspMI	ACCTGC	1 cut(s)	204
BspOI	GCTAGC	1 cut(s)	246
BspT107I	GGYRCC	2 cut(s)	1087, 1131
BspTI	CTTAAG	1 cut(s)	14
BsrBI	CCGCTC	2 cut(s)	2339, 2815
BsrDI	GCAATG	1 cut(s)	1864
BsrFI	RCCGGY	2 cut(s)	706, 1134
BsrI	ACTGG	3 cut(s)	422, 1643, 2155
BssAI	RCCGGY	2 cut(s)	706, 1134
BssECI	CCNNGG	8 cut(s)	262, 742, 867, 1307, 1537, 2044, 2806, 2889
BssHII	GCGCGC	2 cut(s)	39, 1594
BssNAI	GTATAC	1 cut(s)	2227
BssSI	CACGAG	2 cut(s)	809, 852
BssT1I	CCWWGG	6 cut(s)	262, 742, 867, 2044, 2806, 2889
Bst1107I	GTATAC	1 cut(s)	2227
Bst2BI	CACGAG	2 cut(s)	809, 852
Bst2UI	CCWGG	4 cut(s)	253, 1412, 1539, 2915
Bst6I	CTCTTC	3 cut(s)	748, 1537, 2537
BstAFI	CTTAAG	1 cut(s)	14
BstAPI	GCANNNNNTGC	1 cut(s)	619
BstBAI	YACGTR	1 cut(s)	835
BstDEI	CTNAG	4 cut(s)	1940, 2351, 2449, 2756
BstDSI	CCRYGG	2 cut(s)	867, 2044
BstF5I	GGATG	6 cut(s)	74, 559, 613, 877, 2953, 2978
BstFNI	CGCG	5 cut(s)	41, 342, 1596, 2841, 2850
BstHHI	GCGC	7 cut(s)	41, 43, 342, 1596, 1598, 2841, 2852
BstMAI	GTCTC	3 cut(s)	281, 722, 777
BstMWI	GCNNNNNNNGC	9 cut(s)	599, 619, 670, 1211, 1789, 2251, 2760, 2847, 2930
BstNI	CCWGG	4 cut(s)	253, 1412, 1539, 2915
BstNSI	RCATGY	6 cut(s)	685, 823, 2293, 2377, 2494, 2590
BstPAI	GACNNNNGTC	1 cut(s)	2019
BstSCI	CCNGG	4 cut(s)	251, 1410, 1537, 2913
BstSFI	CTRYAG	6 cut(s)	919, 1584, 1989, 2001, 2463, 2617
BstSLI	GKGCMC	4 cut(s)	624, 1538, 1871, 2676
BstSNI	TACGTA	1 cut(s)	835
BstUI	CGCG	5 cut(s)	41, 342, 1596, 2841, 2850
BstV2I	GAAGAC	2 cut(s)	405, 1433
BstX2I	RGATCY	5 cut(s)	139, 454, 1036, 1468, 2652
BstYI	RGATCY	5 cut(s)	139, 454, 1036, 1468, 2652
BstZ17I	GTATAC	1 cut(s)	2227
Bsu36I	CCTNAGG	1 cut(s)	2756
BsuRI	GGCC	9 cut(s)	218, 375, 768, 1214, 1536, 1792, 2266, 2334, 2623
BtgI	CCRYGG	2 cut(s)	867, 2044
BtrI	CACGTC	1 cut(s)	402
BtsCI	GGATG	6 cut(s)	74, 559, 613, 877, 2953, 2978
BtsI	GCAGTG	1 cut(s)	2237
BtsIMutI	CAGTG	4 cut(s)	2237, 2520, 2580, 2825
BveI	ACCTGC	1 cut(s)	204
CaiI	CAGNNNCTG	3 cut(s)	311, 1649, 2827
CciI	TCATGA	2 cut(s)	501, 568
CfoI	GCGC	7 cut(s)	41, 43, 342, 1596, 1598, 2841, 2852
Cfr10I	RCCGGY	2 cut(s)	706, 1134
Cfr13I	GGNCC	9 cut(s)	255, 531, 1534, 1535, 1912, 2056, 2265, 2622, 3018
CsiI	ACCWGGT	1 cut(s)	1410
Csp6I	GTAC	4 cut(s)	1132, 1397, 1963, 2899
CspAI	ACCGGT	1 cut(s)	706
CviQI	GTAC	4 cut(s)	1132, 1397, 1963, 2899
DdeI	CTNAG	4 cut(s)	1940, 2351, 2449, 2756
EaeI	YGGCCR	2 cut(s)	766, 2332
Eam1104I	CTCTTC	3 cut(s)	748, 1537, 2537
EarI	CTCTTC	3 cut(s)	748, 1537, 2537
EciI	GGCGGA	1 cut(s)	2425
Ecl136II	GAGCTC	1 cut(s)	1813
Eco105I	TACGTA	1 cut(s)	835
Eco130I	CCWWGG	6 cut(s)	262, 742, 867, 2044, 2806, 2889
Eco147I	AGGCCT	2 cut(s)	218, 1214
Eco24I	GRGCYC	2 cut(s)	1538, 1815
Eco32I	GATATC	1 cut(s)	2630
Eco47I	GGWCC	5 cut(s)	255, 531, 1912, 2056, 3018
Eco53kI	GAGCTC	1 cut(s)	1813
Eco57I	CTGAAG	3 cut(s)	1418, 2081, 2838
Eco81I	CCTNAGG	1 cut(s)	2756
Eco88I	CYCGRG	2 cut(s)	20, 2027
EcoICRI	GAGCTC	1 cut(s)	1813
EcoO109I	RGGNCCY	4 cut(s)	255, 1535, 2056, 2265
EcoRI	GAATTC	1 cut(s)	2775
EcoRII	CCWGG	4 cut(s)	251, 1410, 1537, 2913
EcoRV	GATATC	1 cut(s)	2630
EcoT14I	CCWWGG	6 cut(s)	262, 742, 867, 2044, 2806, 2889
EcoT22I	ATGCAT	4 cut(s)	1228, 1831, 2256, 2492
EcoT38I	GRGCYC	2 cut(s)	1538, 1815
ErhI	CCWWGG	6 cut(s)	262, 742, 867, 2044, 2806, 2889
FalI	AAGNNNNNCTT	4 cut(s)	1358, 1390, 1491, 1523
FaqI	GGGAC	5 cut(s)	281, 410, 544, 1085, 1688
FauI	CCCGC	1 cut(s)	1110
FauNDI	CATATG	1 cut(s)	1387
FbaI	TGATCA	1 cut(s)	637
FblI	GTMKAC	2 cut(s)	225, 2226
FokI	GGATG	6 cut(s)	61, 546, 620, 884, 2940, 2965
FriOI	GRGCYC	2 cut(s)	1538, 1815
FspBI	CTAG	5 cut(s)	212, 243, 732, 1808, 2687
GlaI	GCGC	7 cut(s)	40, 42, 341, 1595, 1597, 2840, 2851
GsaI	CCCAGC	1 cut(s)	531
GsuI	CTGGAG	4 cut(s)	235, 405, 1107, 1807
HaeIII	GGCC	9 cut(s)	218, 375, 768, 1214, 1536, 1792, 2266, 2334, 2623
HapII	CCGG	6 cut(s)	282, 605, 707, 1135, 1379, 1915
HhaI	GCGC	7 cut(s)	41, 43, 342, 1596, 1598, 2841, 2852
Hin6I	GCGC	7 cut(s)	39, 41, 340, 1594, 1596, 2839, 2850
HinP1I	GCGC	7 cut(s)	39, 41, 340, 1594, 1596, 2839, 2850
HincII	GTYRAC	2 cut(s)	1654, 2311
HindII	GTYRAC	2 cut(s)	1654, 2311
HpaII	CCGG	6 cut(s)	282, 605, 707, 1135, 1379, 1915
HphI	GGTGA	7 cut(s)	271, 1832, 2135, 2291, 2746, 2804, 2905
HpyCH4IV	ACGT	6 cut(s)	401, 834, 1285, 2107, 2296, 2901
HpyF10VI	GCNNNNNNNGC	9 cut(s)	599, 619, 670, 1211, 1789, 2251, 2760, 2847, 2930
HpyF3I	CTNAG	4 cut(s)	1940, 2351, 2449, 2756
HpySE526I	ACGT	6 cut(s)	401, 834, 1285, 2107, 2296, 2901
HspAI	GCGC	7 cut(s)	39, 41, 340, 1594, 1596, 2839, 2850
Kpn2I	TCCGGA	2 cut(s)	1378, 1914
KpnI	GGTACC	1 cut(s)	1135
Ksp22I	TGATCA	1 cut(s)	637
LmnI	GCTCC	7 cut(s)	91, 1126, 2170, 2341, 2412, 2760, 2812
MabI	ACCWGGT	1 cut(s)	1410
MaeI	CTAG	5 cut(s)	212, 243, 732, 1808, 2687
MaeII	ACGT	6 cut(s)	401, 834, 1285, 2107, 2296, 2901
MaeIII	GTNAC	7 cut(s)	388, 874, 2048, 2292, 2297, 2866, 2902
MbiI	CCGCTC	2 cut(s)	2339, 2815
MfeI	CAATTG	1 cut(s)	1797
MflI	RGATCY	5 cut(s)	139, 454, 1036, 1468, 2652
MhlI	GDGCHC	5 cut(s)	624, 1538, 1815, 1871, 2676
MluCI	AATT	9 cut(s)	909, 1041, 1797, 2184, 2422, 2558, 2562, 2775, 2992
MlyI	GAGTC	4 cut(s)	892, 1107, 1951, 2381
Mph1103I	ATGCAT	4 cut(s)	1228, 1831, 2256, 2492
MroI	TCCGGA	2 cut(s)	1378, 1914
MroXI	GAANNNNTTC	2 cut(s)	486, 2562
MseI	TTAA	8 cut(s)	15, 1026, 1044, 1164, 1629, 2157, 2561, 2831
MslI	CAYNNNNRTG	3 cut(s)	1236, 2513, 2679
MspA1I	CMGCKG	2 cut(s)	1268, 1576
MspCI	CTTAAG	1 cut(s)	14
MspI	CCGG	6 cut(s)	282, 605, 707, 1135, 1379, 1915
MspR9I	CCNGG	4 cut(s)	253, 1412, 1539, 2915
MunI	CAATTG	1 cut(s)	1797
Mva1269I	GAATGC	4 cut(s)	1002, 1231, 1585, 1831
MvaI	CCWGG	4 cut(s)	253, 1412, 1539, 2915
MvnI	CGCG	5 cut(s)	41, 342, 1596, 2841, 2850
MwoI	GCNNNNNNNGC	9 cut(s)	599, 619, 670, 1211, 1789, 2251, 2760, 2847, 2930
NcoI	CCATGG	2 cut(s)	867, 2044
NdeI	CATATG	1 cut(s)	1387
NheI	GCTAGC	1 cut(s)	242
NlaIV	GGNNCC	9 cut(s)	116, 456, 532, 1089, 1122, 1133, 1536, 1913, 2343
NmeAIII	GCCGAG	2 cut(s)	2360, 2689
NmuCI	GTSAC	3 cut(s)	874, 2297, 2866
NsiI	ATGCAT	4 cut(s)	1228, 1831, 2256, 2492
NspI	RCATGY	6 cut(s)	685, 823, 2293, 2377, 2494, 2590
OliI	CACNNNNGTG	1 cut(s)	2513
PaeI	GCATGC	1 cut(s)	685
PagI	TCATGA	2 cut(s)	501, 568
PauI	GCGCGC	2 cut(s)	39, 1594
PceI	AGGCCT	2 cut(s)	218, 1214
PciI	ACATGT	3 cut(s)	2289, 2373, 2586
PctI	GAATGC	4 cut(s)	1002, 1231, 1585, 1831
PdmI	GAANNNNTTC	2 cut(s)	486, 2562
PflMI	CCANNNNNTGG	2 cut(s)	1799, 2920
PinAI	ACCGGT	1 cut(s)	706
PleI	GAGTC	4 cut(s)	891, 1106, 1950, 2381
PpsI	GAGTC	4 cut(s)	891, 1106, 1950, 2381
Ppu21I	YACGTR	1 cut(s)	835
PpuMI	RGGWCCY	2 cut(s)	255, 2056
PscI	ACATGT	3 cut(s)	2289, 2373, 2586
PshAI	GACNNNNGTC	1 cut(s)	2019
PshBI	ATTAAT	1 cut(s)	2561
Psp124BI	GAGCTC	1 cut(s)	1815
Psp5II	RGGWCCY	2 cut(s)	255, 2056
Psp6I	CCWGG	4 cut(s)	251, 1410, 1537, 2913
PspFI	CCCAGC	1 cut(s)	527
PspGI	CCWGG	4 cut(s)	251, 1410, 1537, 2913
PspN4I	GGNNCC	9 cut(s)	116, 456, 532, 1089, 1122, 1133, 1536, 1913, 2343
PspOMI	GGGCCC	1 cut(s)	1534
PspPI	GGNCC	9 cut(s)	255, 531, 1534, 1535, 1912, 2056, 2265, 2622, 3018
PspPPI	RGGWCCY	2 cut(s)	255, 2056
PsrI	GAACNNNNNNTAC	2 cut(s)	2600, 2632
PstNI	CAGNNNCTG	3 cut(s)	311, 1649, 2827
PsuI	RGATCY	5 cut(s)	139, 454, 1036, 1468, 2652
PteI	GCGCGC	2 cut(s)	39, 1594
PvuII	CAGCTG	1 cut(s)	1268
RsaI	GTAC	4 cut(s)	1133, 1398, 1964, 2900
RsaNI	GTAC	4 cut(s)	1132, 1397, 1963, 2899
RseI	CAYNNNNRTG	3 cut(s)	1236, 2513, 2679
SacI	GAGCTC	1 cut(s)	1815
SaqAI	TTAA	8 cut(s)	15, 1026, 1044, 1164, 1629, 2157, 2561, 2831
Sau96I	GGNCC	9 cut(s)	255, 531, 1534, 1535, 1912, 2056, 2265, 2622, 3018
SchI	GAGTC	4 cut(s)	892, 1107, 1951, 2381
ScrFI	CCNGG	4 cut(s)	253, 1412, 1539, 2915
SduI	GDGCHC	5 cut(s)	624, 1538, 1815, 1871, 2676
SexAI	ACCWGGT	1 cut(s)	1410
SfcI	CTRYAG	6 cut(s)	919, 1584, 1989, 2001, 2463, 2617
SinI	GGWCC	5 cut(s)	255, 531, 1912, 2056, 3018
SmiMI	CAYNNNNRTG	3 cut(s)	1236, 2513, 2679
SmlI	CTYRAG	4 cut(s)	14, 377, 1139, 1684
SmoI	CTYRAG	4 cut(s)	14, 377, 1139, 1684
SnaBI	TACGTA	1 cut(s)	835
SphI	GCATGC	1 cut(s)	685
Sse9I	AATT	9 cut(s)	909, 1041, 1797, 2184, 2422, 2558, 2562, 2775, 2992
SseBI	AGGCCT	2 cut(s)	218, 1214
SspMI	CTAG	5 cut(s)	212, 243, 732, 1808, 2687
SstI	GAGCTC	1 cut(s)	1815
StuI	AGGCCT	2 cut(s)	218, 1214
StyD4I	CCNGG	4 cut(s)	251, 1410, 1537, 2913
StyI	CCWWGG	6 cut(s)	262, 742, 867, 2044, 2806, 2889
TaiI	ACGT	6 cut(s)	404, 837, 1288, 2110, 2299, 2904
TaqI	TCGA	7 cut(s)	994, 1074, 1338, 1702, 2013, 2700, 3014
TaqII	GACCGA	1 cut(s)	3035
TasI	AATT	9 cut(s)	909, 1041, 1797, 2184, 2422, 2558, 2562, 2775, 2992
TatI	WGTACW	1 cut(s)	1962
TauI	GCSGC	2 cut(s)	345, 1692
Tru1I	TTAA	8 cut(s)	15, 1026, 1044, 1164, 1629, 2157, 2561, 2831
Tru9I	TTAA	8 cut(s)	15, 1026, 1044, 1164, 1629, 2157, 2561, 2831
TscAI	CASTG	4 cut(s)	2244, 2520, 2580, 2832
TseFI	GTSAC	3 cut(s)	874, 2297, 2866
Tsp45I	GTSAC	3 cut(s)	874, 2297, 2866
TspGWI	ACGGA	2 cut(s)	795, 882
TspRI	CASTG	4 cut(s)	2244, 2520, 2580, 2832
Van91I	CCANNNNNTGG	2 cut(s)	1799, 2920
Vha464I	CTTAAG	1 cut(s)	14
VneI	GTGCAC	1 cut(s)	2672
VpaK11BI	GGWCC	5 cut(s)	255, 531, 1912, 2056, 3018
VspI	ATTAAT	1 cut(s)	2561
XapI	RAATTY	2 cut(s)	2422, 2775
XceI	RCATGY	6 cut(s)	685, 823, 2293, 2377, 2494, 2590
XcmI	CCANNNNNNNNNTGG	1 cut(s)	85
XmiI	GTMKAC	2 cut(s)	225, 2226
XmnI	GAANNNNTTC	2 cut(s)	486, 2562
XspI	CTAG	5 cut(s)	212, 243, 732, 1808, 2687
Zsp2I	ATGCAT	4 cut(s)	1228, 1831, 2256, 2492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.