Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001985

Physical Location & Seq

Genomic Coordinates Reverse (-)

27634426 .. 27636743

Gene Structure 2318 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1132200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1635 bp

ATGCTGCCGAGGCTGAACGGTGGTGTTTGGATGGACGACAGGGAGGACAGAGAGACTGTTTCCTGGACTAGAACCTCCGCCGCTGCCATTGCCGGCACCACCGAGAACAAAGACGAGATGGGTTGTCTGTCCACCTTCAAATCGATGATGGAGGTTGAAGACGACTGGTACATGGCGGCTAATAACAACATCCAGGGCCATTCGGATGTCAGAGACATCAGCTTCTCCCCGAGCTTTGCTGACCCAGAAAGCTTGTTGCTCAACCCGGTGGACTCTTCCTCCTCCTGCTCGCCGTCGTCGTCGGTTTTCAACAATCTCGACCCAAATCAGGTTCATTACTACATGCCTCAAAATCCCAACTTGTCTTCGCTGCTTAATGTTGTTCCTAACAACCCTTTGGATCACGGCTTCGATTTGGGCTGTGACATTGGGTTTCTTGACACTCAAGCTTCGACTGGTGGTTCGAGTTTGATGAACAGGGGAGGTGGGGTTTTGACTGGGTTCAATGATTTGAGCTCAAATAGCCAGATGAATGCTCAGAATTTGGGTTCTAATTTACAGTTTTCGACTACCCGTATGCCTCAATTGCTCGAAAACAGTTCAAATTTTTCGGGCTTTCAAGGTTTGGATGATGGTTCAGCCAATGCTTTGTTTCCTAATAGGCCTAAGTTACTGAGACCCCTTGAGTCCTTCCCTTCTGTGGGAGCACAGCCAACTCTTTTCCAGAAAAGAGCGGCGCTTCGGAAAAACTTAGCCGACGGTGGAAGGAATTTGGGTGTTTTGGGCTCACAAGGGGGCCTGGTTTTGAATGAAGGGAATGAGAGGAAGAGGGAGATGGGTGTTGAGAATGAGAAGAAGAGGAAAATGAGCGGGGGAGATGATGTTGATGATCTGAGTTTTGATGGTTCAGGTTTGAACTATGATTCGGATGAGTTTACTGAGAACACTAAGGTGGATGAGGGTGCCAAGAATGGTGGGAATAGCTCGAATGCGAAAAGTTTTGTTACTGCTGCTGGAGGAGGAGGAGGAGGGGGTCGTAAGGGGAAGAAGAAAGGGTTGCCTGCTAAGAATCTGATGGCTGAGAGGCGCCGCCGGCAGAAGCTCAATGATAGGCTGTACATGCTGAGATCCGTTGTTCCGAAGATCAGCAAAATGGACAGGGCCTCAATCCTTGGGGATGCAATTGAGTACTTGAAGGAACTTCTGCAGAGGATCAACAACCTCCATAATGAATTGGAGTCCATCCCTCCCGGGTCTGCATTGACACCTACCGGAAATACTTTCCACCCTTTGACACCCACTCCGGCTACCCTGCCAAATCGTATCAAGGAAGAACTTTGCCCCAGCTCATTACCTAGCCCAAATGGCCAACCTGCAAGGGTTGAAGTTAGGCAGCGAGAAGGACGAGCAGTGAATATCCACATGTTTTGCGGCCGAAGGCCAGGTCTGTTACTCTCGACCATGAGAACTTTGGATAACCTTGGGTTAGACATCCAGCAAGCTGTCATCAGCTGTTTCAATGGTTTTGCTATGGATGTCTTCCGTGCCGAGCAATGCATGGAAGGCCAGGACGTCCACCCGGATCAGATAAAGGCAGTACTTCTGGATTTGATCGGGTTCCATGGCATGGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

545

Amino Acids

59.09

Weight (kDa)

5.65

Isoelectric Point (pI)

50.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH_SAC51	PF23173	358 - 407	6e-07	Transcription factor SAC51 bHLH domain
HLH	PF00010	359 - 401	1.5e-09	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	461 - 537	4.9e-12	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12860 AT1G12860 AT1G12860 AT3G26744 AT3G26744 AT3G26744 AT3G26744 AT3G26744
fragaria_vesca	FvH4_5g09300 FvH4_5g09300
malus_domestica	MD06G1132200.v1.1 MD09G1003800.v1.1 MD14G1148600.v1.1 MD17G1007400.v1.1
prunus_persica	Prupe.3G312800_v2.0.a1 Prupe.3G312800_v2.0.a1 Prupe.5G143200_v2.0.a1
pyrus_communis	pycom06g12380 pycom111g00280 pycom14g12340 pycom17g00300
rosa_chinensis	RchiOBHm_Chr2g0176421 RchiOBHm_Chr7g0188921
rosa_laevigata	RLG00000004651 RLG00000022412
rosa_multiflora	Rmu_co8326225.1_g000001 Rmu_co8487291.1_g000001 Rmu_sc0009140.1_g000010 Rmu_sc0011904.1_g000015 Rmu_ssc0000113.1_g000008
rosa_roxburghii	Rroxscaffold_2G00076450 Rroxscaffold_3G00265940
rosa_rugosa	Rorug02G0594200 Rorug02G0594200 Rorug06G0494600
rosa_samantha	Rh2AG672700 Rh2BG684200 Rh2CG647000 Rh2DG697800 Rh7AG099500 Rh7BG101600 Rh7CG102900 Rh7DG101300
rosa_wichuraiana	Rw2G055180 Rw7G008570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1575
Acc36I	ACCTGC	1 cut(s)	1381
AccB1I	GGYRCC	3 cut(s)	95, 962, 1086
AccB7I	CCANNNNNTGG	1 cut(s)	1627
AccBSI	CCGCTC	2 cut(s)	734, 870
AciI	CCGC	7 cut(s)	78, 81, 176, 734, 870, 1090, 1431
AclWI	GGATC	4 cut(s)	408, 1122, 1220, 1590
AcoI	YGGCCR	2 cut(s)	1366, 1432
AcsI	RAATTY	3 cut(s)	541, 604, 769
AcyI	GRCGYC	2 cut(s)	1087, 1572
AfaI	GTAC	4 cut(s)	170, 1118, 1190, 1599
AfiI	CCNNNNNNNGG	4 cut(s)	328, 700, 701, 1627
AflIII	ACRYGT	1 cut(s)	1422
AjnI	CCWGG	5 cut(s)	62, 192, 798, 1441, 1566
AleI	CACNNNNGTG	1 cut(s)	950
Alw21I	GWGCWC	2 cut(s)	518, 709
Alw26I	GTCTC	3 cut(s)	47, 207, 670
AlwI	GGATC	4 cut(s)	408, 1122, 1220, 1590
Ama87I	CYCGRG	2 cut(s)	229, 1250
AoxI	GGCC	8 cut(s)	196, 662, 796, 1161, 1366, 1432, 1439, 1564
ApeKI	GCWGC	5 cut(s)	4, 83, 370, 1010, 1393
ApoI	RAATTY	3 cut(s)	541, 604, 769
ArsI	GACNNNNNNTTYG	4 cut(s)	445, 477, 1429, 1461
AspLEI	GCGC	2 cut(s)	739, 1089
AspS9I	GGNCC	3 cut(s)	196, 796, 1161
AsuC2I	CCSGG	4 cut(s)	266, 1251, 1252, 1580
AvaI	CYCGRG	2 cut(s)	229, 1250
BalI	TGGCCA	1 cut(s)	1368
BanI	GGYRCC	3 cut(s)	95, 962, 1086
BanII	GRGCYC	2 cut(s)	518, 788
BbsI	GAAGAC	3 cut(s)	165, 357, 1531
Bbv12I	GWGCWC	2 cut(s)	518, 709
BbvI	GCAGC	4 cut(s)	70, 357, 997, 1405
BccI	CCATC	8 cut(s)	25, 112, 142, 626, 829, 896, 1069, 1250
BceAI	ACGGC	2 cut(s)	277, 421
BciT130I	CCWGG	5 cut(s)	64, 194, 800, 1443, 1568
BcnI	CCSGG	4 cut(s)	266, 1251, 1252, 1580
BcoDI	GTCTC	3 cut(s)	47, 207, 670
BfaI	CTAG	2 cut(s)	69, 1356
BfmI	CTRYAG	1 cut(s)	1205
BfoI	RGCGCY	2 cut(s)	740, 1090
BfuAI	ACCTGC	1 cut(s)	1381
BglI	GCCNNNNNGGC	1 cut(s)	1365
BmcAI	AGTACT	2 cut(s)	1190, 1599
Bme1390I	CCNGG	9 cut(s)	64, 194, 266, 800, 1251, 1252, 1443, 1568, 1580
BmeT110I	CYCGRG	2 cut(s)	229, 1250
BmgT120I	GGNCC	3 cut(s)	196, 796, 1161
BmiI	GGNNCC	5 cut(s)	97, 797, 964, 1088, 1619
BmrFI	CCNGG	9 cut(s)	64, 194, 266, 800, 1251, 1252, 1443, 1568, 1580
BmrI	ACTGGG	1 cut(s)	507
BmsI	GCATC	1 cut(s)	1168
BmuI	ACTGGG	1 cut(s)	507
BpiI	GAAGAC	3 cut(s)	165, 357, 1531
BpmI	CTGGAG	1 cut(s)	1035
BpuEI	CTTGAG	2 cut(s)	429, 704
BpuMI	CCSGG	4 cut(s)	266, 1251, 1252, 1580
Bsa29I	ATCGAT	1 cut(s)	143
BsaHI	GRCGYC	2 cut(s)	1087, 1572
BsaI	GGTCTC	1 cut(s)	670
BsaJI	CCNNGG	6 cut(s)	8, 193, 1171, 1250, 1480, 1621
BsaWI	WCCGGW	1 cut(s)	1271
BsaXI	ACNNNNNCTCC	8 cut(s)	263, 293, 474, 504, 867, 897, 1285, 1315
Bsc4I	CCNNNNNNNGG	4 cut(s)	328, 700, 701, 1627
Bse118I	RCCGGY	2 cut(s)	92, 1092
Bse1I	ACTGG	3 cut(s)	170, 460, 502
Bse3DI	GCAATG	2 cut(s)	87, 1559
BseBI	CCWGG	5 cut(s)	64, 194, 800, 1443, 1568
BseCI	ATCGAT	1 cut(s)	143
BseDI	CCNNGG	6 cut(s)	8, 193, 1171, 1250, 1480, 1621
BseLI	CCNNNNNNNGG	4 cut(s)	328, 700, 701, 1627
BseMI	GCAATG	2 cut(s)	87, 1559
BseMII	CTCAG	6 cut(s)	551, 665, 884, 930, 1071, 1115
BseNI	ACTGG	3 cut(s)	170, 460, 502
BseRI	GAGGAG	5 cut(s)	271, 1032, 1035, 1038, 1041
BseX3I	CGGCCG	1 cut(s)	1432
BseXI	GCAGC	4 cut(s)	70, 357, 997, 1405
BseYI	CCCAGC	1 cut(s)	1343
Bsh1285I	CGRYCG	1 cut(s)	1435
BshFI	GGCC	8 cut(s)	198, 664, 798, 1163, 1368, 1434, 1441, 1566
BshNI	GGYRCC	3 cut(s)	95, 962, 1086
BshVI	ATCGAT	1 cut(s)	143
BsiEI	CGRYCG	1 cut(s)	1435
BsiHKAI	GWGCWC	2 cut(s)	518, 709
BsiHKCI	CYCGRG	2 cut(s)	229, 1250
BsiSI	CCGG	7 cut(s)	93, 266, 1093, 1251, 1272, 1304, 1580
BslI	CCNNNNNNNGG	4 cut(s)	328, 700, 701, 1627
BsmAI	GTCTC	3 cut(s)	47, 207, 670
BsmI	GAATGC	2 cut(s)	538, 994
BsnI	GGCC	8 cut(s)	198, 664, 798, 1163, 1368, 1434, 1441, 1566
Bso31I	GGTCTC	1 cut(s)	670
BsoBI	CYCGRG	2 cut(s)	229, 1250
Bsp1286I	GDGCHC	3 cut(s)	518, 709, 788
Bsp1407I	TGTACA	1 cut(s)	1116
Bsp143I	GATC	7 cut(s)	400, 889, 1127, 1143, 1212, 1582, 1611
Bsp19I	CCATGG	1 cut(s)	1621
BspACI	CCGC	7 cut(s)	78, 81, 176, 734, 870, 1090, 1431
BspANI	GGCC	8 cut(s)	198, 664, 798, 1163, 1368, 1434, 1441, 1566
BspCNI	CTCAG	6 cut(s)	550, 666, 885, 931, 1072, 1116
BspDI	ATCGAT	1 cut(s)	143
BspLI	GGNNCC	5 cut(s)	97, 797, 964, 1088, 1619
BspMAI	CTGCAG	1 cut(s)	1209
BspMI	ACCTGC	1 cut(s)	1381
BspPI	GGATC	4 cut(s)	408, 1122, 1220, 1590
BspT107I	GGYRCC	3 cut(s)	95, 962, 1086
BspTNI	GGTCTC	1 cut(s)	670
BsrBI	CCGCTC	2 cut(s)	734, 870
BsrDI	GCAATG	2 cut(s)	87, 1559
BsrFI	RCCGGY	2 cut(s)	92, 1092
BsrGI	TGTACA	1 cut(s)	1116
BsrI	ACTGG	3 cut(s)	170, 460, 502
BssAI	RCCGGY	2 cut(s)	92, 1092
BssECI	CCNNGG	6 cut(s)	8, 193, 1171, 1250, 1480, 1621
BssMI	GATC	7 cut(s)	400, 889, 1127, 1143, 1212, 1582, 1611
BssNI	GRCGYC	2 cut(s)	1087, 1572
BssT1I	CCWWGG	3 cut(s)	1171, 1480, 1621
Bst2UI	CCWGG	5 cut(s)	64, 194, 800, 1443, 1568
Bst4CI	ACNGT	5 cut(s)	20, 58, 561, 599, 761
Bst6I	CTCTTC	3 cut(s)	280, 821, 851
BstACI	GRCGYC	2 cut(s)	1087, 1572
BstAUI	TGTACA	1 cut(s)	1116
BstC8I	GCNNGC	5 cut(s)	94, 290, 1062, 1094, 1500
BstDSI	CCRYGG	1 cut(s)	1621
BstH2I	RGCGCY	2 cut(s)	740, 1090
BstHHI	GCGC	2 cut(s)	739, 1089
BstKTI	GATC	7 cut(s)	403, 892, 1130, 1146, 1215, 1585, 1614
BstMAI	GTCTC	3 cut(s)	47, 207, 670
BstMBI	GATC	7 cut(s)	400, 889, 1127, 1143, 1212, 1582, 1611
BstMCI	CGRYCG	1 cut(s)	1435
BstMWI	GCNNNNNNNGC	8 cut(s)	10, 89, 522, 586, 1093, 1120, 1365, 1563
BstNI	CCWGG	5 cut(s)	64, 194, 800, 1443, 1568
BstNSI	RCATGY	3 cut(s)	346, 1123, 1426
BstSCI	CCNGG	9 cut(s)	62, 192, 264, 798, 1249, 1250, 1441, 1566, 1578
BstSFI	CTRYAG	1 cut(s)	1205
BstV1I	GCAGC	4 cut(s)	70, 357, 997, 1405
BstV2I	GAAGAC	3 cut(s)	165, 357, 1531
BstX2I	RGATCY	1 cut(s)	1127
BstYI	RGATCY	1 cut(s)	1127
BstZI	CGGCCG	1 cut(s)	1432
Bsu15I	ATCGAT	1 cut(s)	143
BsuRI	GGCC	8 cut(s)	198, 664, 798, 1163, 1368, 1434, 1441, 1566
BsuTUI	ATCGAT	1 cut(s)	143
BtgI	CCRYGG	1 cut(s)	1621
BtsI	GCAGTG	1 cut(s)	1416
BtsIMutI	CAGTG	1 cut(s)	1416
BveI	ACCTGC	1 cut(s)	1381
Cac8I	GCNNGC	5 cut(s)	94, 290, 1062, 1094, 1500
CfoI	GCGC	2 cut(s)	739, 1089
Cfr10I	RCCGGY	2 cut(s)	92, 1092
Cfr13I	GGNCC	3 cut(s)	196, 796, 1161
Cfr9I	CCCGGG	1 cut(s)	1250
ClaI	ATCGAT	1 cut(s)	143
Csp6I	GTAC	4 cut(s)	169, 1117, 1189, 1598
CspCI	CAANNNNNGTGG	4 cut(s)	955, 990, 1409, 1444
CviAII	CATG	8 cut(s)	172, 343, 1120, 1423, 1462, 1558, 1622, 1627
CviQI	GTAC	4 cut(s)	169, 1117, 1189, 1598
DinI	GGCGCC	1 cut(s)	1088
DpnI	GATC	7 cut(s)	402, 891, 1129, 1145, 1214, 1584, 1613
DpnII	GATC	7 cut(s)	400, 889, 1127, 1143, 1212, 1582, 1611
EaeI	YGGCCR	2 cut(s)	1366, 1432
EagI	CGGCCG	1 cut(s)	1432
Eam1104I	CTCTTC	3 cut(s)	280, 821, 851
EarI	CTCTTC	3 cut(s)	280, 821, 851
EciI	GGCGGA	1 cut(s)	67
Ecl136II	GAGCTC	1 cut(s)	516
EclXI	CGGCCG	1 cut(s)	1432
Eco130I	CCWWGG	3 cut(s)	1171, 1480, 1621
Eco147I	AGGCCT	1 cut(s)	664
Eco24I	GRGCYC	2 cut(s)	518, 788
Eco31I	GGTCTC	1 cut(s)	670
Eco52I	CGGCCG	1 cut(s)	1432
Eco53kI	GAGCTC	1 cut(s)	516
Eco88I	CYCGRG	2 cut(s)	229, 1250
EcoICRI	GAGCTC	1 cut(s)	516
EcoO109I	RGGNCCY	2 cut(s)	796, 1161
EcoRII	CCWGG	5 cut(s)	62, 192, 798, 1441, 1566
EcoT14I	CCWWGG	3 cut(s)	1171, 1480, 1621
EcoT22I	ATGCAT	1 cut(s)	1559
EcoT38I	GRGCYC	2 cut(s)	518, 788
EgeI	GGCGCC	1 cut(s)	1088
EheI	GGCGCC	1 cut(s)	1088
ErhI	CCWWGG	3 cut(s)	1171, 1480, 1621
FaeI	CATG	8 cut(s)	175, 346, 1123, 1426, 1465, 1561, 1625, 1630
FatI	CATG	8 cut(s)	171, 342, 1119, 1422, 1461, 1557, 1621, 1626
FauI	CCCGC	1 cut(s)	863
FriOI	GRGCYC	2 cut(s)	518, 788
FspBI	CTAG	2 cut(s)	69, 1356
GlaI	GCGC	2 cut(s)	738, 1088
GsaI	CCCAGC	1 cut(s)	1347
GsuI	CTGGAG	1 cut(s)	1035
HaeII	RGCGCY	2 cut(s)	740, 1090
HaeIII	GGCC	8 cut(s)	198, 664, 798, 1163, 1368, 1434, 1441, 1566
HapII	CCGG	7 cut(s)	93, 266, 1093, 1251, 1272, 1304, 1580
HhaI	GCGC	2 cut(s)	739, 1089
Hin1I	GRCGYC	2 cut(s)	1087, 1572
Hin1II	CATG	8 cut(s)	175, 346, 1123, 1426, 1465, 1561, 1625, 1630
Hin6I	GCGC	2 cut(s)	737, 1087
HinP1I	GCGC	2 cut(s)	737, 1087
HindIII	AAGCTT	2 cut(s)	250, 447
HinfI	GANTC	5 cut(s)	272, 686, 923, 1069, 1238
HpaII	CCGG	7 cut(s)	93, 266, 1093, 1251, 1272, 1304, 1580
Hpy166II	GTNNAC	4 cut(s)	132, 271, 936, 1576
Hpy188I	TCNGA	9 cut(s)	205, 212, 540, 744, 894, 928, 1074, 1140, 1587
Hpy188III	TCNNGA	5 cut(s)	317, 437, 724, 1456, 1604
Hpy8I	GTNNAC	4 cut(s)	132, 271, 936, 1576
Hpy99I	CGWCG	4 cut(s)	298, 301, 304, 761
HpyAV	CCTTC	9 cut(s)	145, 700, 705, 759, 806, 1189, 1394, 1431, 1556
HpyCH4III	ACNGT	5 cut(s)	20, 58, 561, 599, 761
HpyCH4IV	ACGT	1 cut(s)	1572
HpyCH4V	TGCA	5 cut(s)	1181, 1207, 1259, 1376, 1557
HpyF10VI	GCNNNNNNNGC	8 cut(s)	10, 89, 522, 586, 1093, 1120, 1365, 1563
HpySE526I	ACGT	1 cut(s)	1572
Hsp92I	GRCGYC	2 cut(s)	1087, 1572
Hsp92II	CATG	8 cut(s)	175, 346, 1123, 1426, 1465, 1561, 1625, 1630
HspAI	GCGC	2 cut(s)	737, 1087
KasI	GGCGCC	1 cut(s)	1086
KroI	GCCGGC	2 cut(s)	92, 1092
KroNI	GCCGGC	2 cut(s)	94, 1094
Kzo9I	GATC	7 cut(s)	400, 889, 1127, 1143, 1212, 1582, 1611
LmnI	GCTCC	1 cut(s)	704
Lsp1109I	GCAGC	4 cut(s)	70, 357, 997, 1405
LweI	GCATC	1 cut(s)	1168
MaeI	CTAG	2 cut(s)	69, 1356
MaeII	ACGT	1 cut(s)	1572
MaeIII	GTNAC	4 cut(s)	422, 669, 1003, 1449
MalI	GATC	7 cut(s)	402, 891, 1129, 1145, 1214, 1584, 1613
MbiI	CCGCTC	2 cut(s)	734, 870
MboI	GATC	7 cut(s)	400, 889, 1127, 1143, 1212, 1582, 1611
MfeI	CAATTG	2 cut(s)	584, 1182
MflI	RGATCY	1 cut(s)	1127
MhlI	GDGCHC	3 cut(s)	518, 709, 788
MlsI	TGGCCA	1 cut(s)	1368
MluCI	AATT	7 cut(s)	541, 553, 584, 604, 769, 1182, 1232
MluNI	TGGCCA	1 cut(s)	1368
Mly113I	GGCGCC	1 cut(s)	1087
MlyI	GAGTC	3 cut(s)	266, 695, 1247
Mox20I	TGGCCA	1 cut(s)	1368
Mph1103I	ATGCAT	1 cut(s)	1559
MroNI	GCCGGC	2 cut(s)	92, 1092
MscI	TGGCCA	1 cut(s)	1368
MseI	TTAA	1 cut(s)	375
MslI	CAYNNNNRTG	2 cut(s)	204, 950
Msp20I	TGGCCA	1 cut(s)	1368
MspA1I	CMGCKG	2 cut(s)	83, 1512
MspI	CCGG	7 cut(s)	93, 266, 1093, 1251, 1272, 1304, 1580
MspR9I	CCNGG	9 cut(s)	64, 194, 266, 800, 1251, 1252, 1443, 1568, 1580
MunI	CAATTG	2 cut(s)	584, 1182
Mva1269I	GAATGC	2 cut(s)	538, 994
MvaI	CCWGG	5 cut(s)	64, 194, 800, 1443, 1568
MwoI	GCNNNNNNNGC	8 cut(s)	10, 89, 522, 586, 1093, 1120, 1365, 1563
NaeI	GCCGGC	2 cut(s)	94, 1094
NarI	GGCGCC	1 cut(s)	1087
NciI	CCSGG	4 cut(s)	266, 1251, 1252, 1580
NcoI	CCATGG	1 cut(s)	1621
NdeII	GATC	7 cut(s)	400, 889, 1127, 1143, 1212, 1582, 1611
NgoMIV	GCCGGC	2 cut(s)	92, 1092
NlaIII	CATG	8 cut(s)	175, 346, 1123, 1426, 1465, 1561, 1625, 1630
NlaIV	GGNNCC	5 cut(s)	97, 797, 964, 1088, 1619
NmeAIII	GCCGAG	2 cut(s)	33, 1573
NmuCI	GTSAC	1 cut(s)	422
NsiI	ATGCAT	1 cut(s)	1559
NspI	RCATGY	3 cut(s)	346, 1123, 1426
OliI	CACNNNNGTG	1 cut(s)	950
PceI	AGGCCT	1 cut(s)	664
PciI	ACATGT	1 cut(s)	1422
PcsI	WCGNNNNNNNCGW	1 cut(s)	296
PctI	GAATGC	2 cut(s)	538, 994
PdiI	GCCGGC	2 cut(s)	94, 1094
PfeI	GAWTC	2 cut(s)	923, 1069
PflMI	CCANNNNNTGG	1 cut(s)	1627
PfoI	TCCNGGA	1 cut(s)	62
PleI	GAGTC	3 cut(s)	266, 694, 1246
PluTI	GGCGCC	1 cut(s)	1090
PpsI	GAGTC	3 cut(s)	266, 694, 1246
PscI	ACATGT	1 cut(s)	1422
Psp124BI	GAGCTC	1 cut(s)	518
Psp6I	CCWGG	5 cut(s)	62, 192, 798, 1441, 1566
PspFI	CCCAGC	1 cut(s)	1343
PspGI	CCWGG	5 cut(s)	62, 192, 798, 1441, 1566
PspN4I	GGNNCC	5 cut(s)	97, 797, 964, 1088, 1619
PspPI	GGNCC	3 cut(s)	196, 796, 1161
PstI	CTGCAG	1 cut(s)	1209
PsuI	RGATCY	1 cut(s)	1127
PvuII	CAGCTG	1 cut(s)	1512
RsaI	GTAC	4 cut(s)	170, 1118, 1190, 1599
RsaNI	GTAC	4 cut(s)	169, 1117, 1189, 1598
RseI	CAYNNNNRTG	2 cut(s)	204, 950
SacI	GAGCTC	1 cut(s)	518
SaqAI	TTAA	1 cut(s)	375
Sau3AI	GATC	7 cut(s)	400, 889, 1127, 1143, 1212, 1582, 1611
Sau96I	GGNCC	3 cut(s)	196, 796, 1161
ScaI	AGTACT	2 cut(s)	1190, 1599
SchI	GAGTC	3 cut(s)	266, 695, 1247
ScrFI	CCNGG	9 cut(s)	64, 194, 266, 800, 1251, 1252, 1443, 1568, 1580
SduI	GDGCHC	3 cut(s)	518, 709, 788
SfaNI	GCATC	1 cut(s)	1168
SfcI	CTRYAG	1 cut(s)	1205
SfoI	GGCGCC	1 cut(s)	1088
SmaI	CCCGGG	1 cut(s)	1252
SmiMI	CAYNNNNRTG	2 cut(s)	204, 950
SmlI	CTYRAG	2 cut(s)	444, 683
SmoI	CTYRAG	2 cut(s)	444, 683
Sse9I	AATT	7 cut(s)	541, 553, 584, 604, 769, 1182, 1232
SseBI	AGGCCT	1 cut(s)	664
SsiI	CCGC	7 cut(s)	78, 81, 176, 734, 870, 1090, 1431
SspDI	GGCGCC	1 cut(s)	1086
SspMI	CTAG	2 cut(s)	69, 1356
SstI	GAGCTC	1 cut(s)	518
StuI	AGGCCT	1 cut(s)	664
StyD4I	CCNGG	9 cut(s)	62, 192, 264, 798, 1249, 1250, 1441, 1566, 1578
StyI	CCWWGG	3 cut(s)	1171, 1480, 1621
TaaI	ACNGT	5 cut(s)	20, 58, 561, 599, 761
TaiI	ACGT	1 cut(s)	1575
TaqI	TCGA	9 cut(s)	143, 318, 411, 452, 464, 566, 591, 986, 1457
TasI	AATT	7 cut(s)	541, 553, 584, 604, 769, 1182, 1232
TatI	WGTACW	3 cut(s)	1116, 1188, 1597
TauI	GCSGC	5 cut(s)	83, 179, 737, 1092, 1434
TfiI	GAWTC	2 cut(s)	923, 1069
Tru1I	TTAA	1 cut(s)	375
Tru9I	TTAA	1 cut(s)	375
TscAI	CASTG	1 cut(s)	1416
TseFI	GTSAC	1 cut(s)	422
TseI	GCWGC	5 cut(s)	4, 83, 370, 1010, 1393
Tsp45I	GTSAC	1 cut(s)	422
TspDTI	ATGAA	5 cut(s)	323, 488, 545, 825, 1245
TspGWI	ACGGA	2 cut(s)	1120, 1532
TspMI	CCCGGG	1 cut(s)	1250
TspRI	CASTG	1 cut(s)	1416
Van91I	CCANNNNNTGG	1 cut(s)	1627
XapI	RAATTY	3 cut(s)	541, 604, 769
XceI	RCATGY	3 cut(s)	346, 1123, 1426
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1468
XmaI	CCCGGG	1 cut(s)	1250
XspI	CTAG	2 cut(s)	69, 1356
ZraI	GACGTC	1 cut(s)	1573
ZrmI	AGTACT	2 cut(s)	1190, 1599
Zsp2I	ATGCAT	1 cut(s)	1559

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.