Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_111

Orthogroup ID

OG0001985

Physical Location & Seq

Genomic Coordinates Reverse (-)

221576 .. 224260

Gene Structure 2685 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom111g00280.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGCTTCCGATGTCGAGCGGCGCCGCTTGGATGGGCGGAGACGAAGACGACGCAGCGTCTTGGACCAGAAACAGCACCACCACCCACAACAACAACAACAACAACGAGGCAGAGCCCAGAAGAAACGACCAAGACTCGAGCTTGGGAGCTTCTTTTTCAAACTTCAAATCAATGCTGGAAGGTGACTGGTACATGAACAACGTTCTCAGCAACCCAGCTCAAGACCTCCATGCCTTCTCTTCCACCCAAGCTTCTGAAACTACACTTGCTCCTCTGCAACCAATCGACTCCTCCGCCTCCTGTTCTCCGTCGCCGGCCTTCTCTCTCGACCCTTCACAGCCGCCACAGCAGTTTTTGCCTCCAAAATCCTGCTTCTCCTCTCTCCTCAACGTCGTCTGCTCCAACCCTTTTGATAACAACTTCGACCTGGGCTGCGACGCTGGGTTTCTGGGCTCCTTTCAGGGAAATCAGCCTTCCAATTCCTCTGTTCTGATGGGTTTCACGGCCCTCAATTCTCATTCTCAGATGGGTACTCCCGAACTCAGTTCGAGCGCCGAGTTTCCAGCCAGTCGGTTGCTTCCGGTAACGGACAACGCCAATGTACTCGACGGCGACTTTGGCTTCGAAGGCTTCGATGGCTCGGCCGGCGCTCAGCTGCTGAACCGGGCTAAGCTCCTGTTCCCTTCCATGGGTGCTCAGCCCACCCTTTTCCAGAAGCGCCGCCAAAACTCTGTAGGCGATGGGGGTGATAAATTGGGAAATTTGGAGATTTCGGGTCCCAGGTACGGGGGACTACTGGAGAGTTTGGAGAAGAAAAGGAAGAGGAACGAGGAGGGTGAGATGGAGGAAGGGAGTCTGGATGTGTCCGGTTTGAATTATGACTCGGATGACTTCAATGAGTACAGTCAAGTGGAGGTGGAGGAGAATGCGAAGAATGGTGGAAGCAATTCGAATGCCAACAGCACTGTCACCGGTGTAGAGGGAGGAGACCGGAAGGGCAAGAAGAAGGGTTTGCCGGCCAAAAATTTGATGGCGGAGAGGCGGCGGAGGAAGAAGCTCAATGATAGGCTCTACATGCTTAGGTCTGTTGTACCCAAGATAAGCAAGATGGATAGGGCGTCCATACTAGGGGATGCAATTGATTATTTGAAAGAGCTTCTACAAAGGATTAATGACCTCCATAACGAGCTGGAGTCAGCTCCACCCGGTTCTTTGCTGCCTGCTTCAACAAGTTTTCATCCGTTGACACCCACTCCATCCACCCTTCCCTGCCGTGTTAAAGAAGAGCTCTGCCCGAGCTCCTTGCTAAGCCCCAAAACTCAGCCGAAGGTGGAGGTTCGGGTAAGGGAAGGGCGAACTGTTAATATCCACATGTTCTGTTCTCGGAGACCAGGTCTCTTACTCTCAACCATGAGGGCCTTGGATAACCTTGATTTGGACGTCCAGCAGGCTGTGATCAGCTGCTTCAATGGGTTTGCTTTAGATGTGTTCCGAGCTGAGCAATGCAGGGAAAACCAGTTCTTGCCAGAGCAAATAAAAGCAGTACTTTTGGATTCAGCTGGATTTCATGATATGATGATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

528

Amino Acids

57.48

Weight (kDa)

5.09

Isoelectric Point (pI)

53.69

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12860 AT1G12860 AT1G12860 AT3G26744 AT3G26744 AT3G26744 AT3G26744 AT3G26744
fragaria_vesca	FvH4_5g09300 FvH4_5g09300
malus_domestica	MD06G1132200.v1.1 MD09G1003800.v1.1 MD14G1148600.v1.1 MD17G1007400.v1.1
prunus_persica	Prupe.3G312800_v2.0.a1 Prupe.3G312800_v2.0.a1 Prupe.5G143200_v2.0.a1
pyrus_communis	pycom06g12380 pycom111g00280 pycom14g12340 pycom17g00300
rosa_chinensis	RchiOBHm_Chr2g0176421 RchiOBHm_Chr7g0188921
rosa_laevigata	RLG00000004651 RLG00000022412
rosa_multiflora	Rmu_co8326225.1_g000001 Rmu_co8487291.1_g000001 Rmu_sc0009140.1_g000010 Rmu_sc0011904.1_g000015 Rmu_ssc0000113.1_g000008
rosa_roxburghii	Rroxscaffold_2G00076450 Rroxscaffold_3G00265940
rosa_rugosa	Rorug02G0594200 Rorug02G0594200 Rorug06G0494600
rosa_samantha	Rh2AG672700 Rh2BG684200 Rh2CG647000 Rh2DG697800 Rh7AG099500 Rh7BG101600 Rh7CG102900 Rh7DG101300
rosa_wichuraiana	Rw2G055180 Rw7G008570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1443
AccB1I	GGYRCC	1 cut(s)	20
AccBSI	CCGCTC	1 cut(s)	18
AciI	CCGC	9 cut(s)	18, 24, 36, 294, 341, 721, 1034, 1042, 1045
AclI	AACGTT	1 cut(s)	201
AcoI	YGGCCR	2 cut(s)	642, 1017
AcsI	RAATTY	2 cut(s)	760, 1024
AcyI	GRCGYC	3 cut(s)	21, 1118, 1440
AfaI	GTAC	7 cut(s)	191, 532, 603, 785, 902, 1092, 1545
AfiI	CCNNNNNNNGG	6 cut(s)	688, 689, 785, 786, 1128, 1435
AflIII	ACRYGT	1 cut(s)	1371
AgeI	ACCGGT	1 cut(s)	971
AgsI	TTSAA	7 cut(s)	159, 166, 874, 895, 1150, 1227, 1468
AhdI	GACNNNNNGTC	1 cut(s)	55
AjnI	CCWGG	3 cut(s)	426, 779, 1390
AloI	GAACNNNNNNTCC	2 cut(s)	1502, 1534
Alw21I	GWGCWC	3 cut(s)	697, 1290, 1301
Alw26I	GTCTC	4 cut(s)	33, 981, 1381, 1400
AlwNI	CAGNNNCTG	1 cut(s)	658
Ama87I	CYCGRG	2 cut(s)	136, 1294
AoxI	GGCC	5 cut(s)	315, 504, 642, 1017, 1416
ApeKI	GCWGC	5 cut(s)	53, 432, 655, 1216, 1461
ApoI	RAATTY	2 cut(s)	760, 1024
ArsI	GACNNNNNNTTYG	4 cut(s)	599, 605, 631, 637
AseI	ATTAAT	1 cut(s)	1170
AsiGI	ACCGGT	1 cut(s)	971
Asp700I	GAANNNNTTC	1 cut(s)	946
AspLEI	GCGC	4 cut(s)	23, 554, 650, 720
AspS9I	GGNCC	4 cut(s)	63, 505, 776, 1416
AsuC2I	CCSGG	2 cut(s)	665, 1206
AsuHPI	GGTGA	4 cut(s)	194, 758, 848, 961
AsuII	TTCGAA	2 cut(s)	624, 950
AvaI	CYCGRG	2 cut(s)	136, 1294
AvaII	GGWCC	2 cut(s)	63, 776
BanI	GGYRCC	1 cut(s)	20
BanII	GRGCYC	4 cut(s)	117, 455, 1290, 1301
BbsI	GAAGAC	1 cut(s)	51
Bbv12I	GWGCWC	3 cut(s)	697, 1290, 1301
BbvI	GCAGC	5 cut(s)	65, 419, 642, 1203, 1448
BccI	CCATC	9 cut(s)	25, 487, 520, 629, 734, 835, 1024, 1102, 1264
BceAI	ACGGC	3 cut(s)	519, 625, 1257
BcgI	CGANNNNNNTGC	2 cut(s)	1285, 1319
BciT130I	CCWGG	3 cut(s)	428, 781, 1392
BclI	TGATCA	1 cut(s)	1455
BcnI	CCSGG	2 cut(s)	665, 1206
BcoDI	GTCTC	4 cut(s)	33, 981, 1381, 1400
BfaI	CTAG	1 cut(s)	1127
BfmI	CTRYAG	1 cut(s)	732
BfoI	RGCGCY	4 cut(s)	24, 555, 651, 721
BlpI	GCTNAGC	5 cut(s)	651, 669, 696, 1307, 1497
BmcAI	AGTACT	1 cut(s)	1545
Bme1390I	CCNGG	5 cut(s)	428, 665, 781, 1206, 1392
Bme18I	GGWCC	2 cut(s)	63, 776
BmeRI	GACNNNNNGTC	1 cut(s)	55
BmeT110I	CYCGRG	2 cut(s)	136, 1294
BmgT120I	GGNCC	4 cut(s)	63, 505, 776, 1416
BmiI	GGNNCC	4 cut(s)	22, 454, 777, 778
BmrFI	CCNGG	5 cut(s)	428, 665, 781, 1206, 1392
BmsI	GCATC	1 cut(s)	1123
BpiI	GAAGAC	1 cut(s)	51
BpmI	CTGGAG	2 cut(s)	818, 1211
Bpu10I	CCTNAGC	1 cut(s)	1079
Bpu1102I	GCTNAGC	5 cut(s)	651, 669, 696, 1307, 1497
Bpu14I	TTCGAA	2 cut(s)	624, 950
BpuEI	CTTGAG	1 cut(s)	204
BpuMI	CCSGG	2 cut(s)	665, 1206
BsaHI	GRCGYC	3 cut(s)	21, 1118, 1440
BsaI	GGTCTC	3 cut(s)	981, 1381, 1400
BsaJI	CCNNGG	4 cut(s)	427, 687, 779, 1419
BsaWI	WCCGGW	4 cut(s)	580, 866, 971, 990
BsaXI	ACNNNNNCTCC	6 cut(s)	253, 283, 1237, 1267, 1378, 1408
Bsc4I	CCNNNNNNNGG	6 cut(s)	688, 689, 785, 786, 1128, 1435
Bse118I	RCCGGY	4 cut(s)	313, 644, 971, 1015
Bse1I	ACTGG	4 cut(s)	191, 567, 801, 1516
Bse3DI	GCAATG	1 cut(s)	1508
BseBI	CCWGG	3 cut(s)	428, 781, 1392
BseDI	CCNNGG	4 cut(s)	427, 687, 779, 1419
BseGI	GGATG	6 cut(s)	36, 865, 892, 1138, 1237, 1256
BseLI	CCNNNNNNNGG	6 cut(s)	688, 689, 785, 786, 1128, 1435
BseMI	GCAATG	1 cut(s)	1508
BseMII	CTCAG	7 cut(s)	220, 536, 556, 665, 710, 1334, 1488
BseNI	ACTGG	4 cut(s)	191, 567, 801, 1516
BseRI	GAGGAG	7 cut(s)	261, 280, 367, 374, 845, 935, 999
BseX3I	CGGCCG	1 cut(s)	642
BseXI	GCAGC	5 cut(s)	65, 419, 642, 1203, 1448
BseYI	CCCAGC	2 cut(s)	214, 440
Bsh1285I	CGRYCG	1 cut(s)	645
BshFI	GGCC	5 cut(s)	317, 506, 644, 1019, 1418
BshNI	GGYRCC	1 cut(s)	20
BshTI	ACCGGT	1 cut(s)	971
BsiEI	CGRYCG	1 cut(s)	645
BsiHKAI	GWGCWC	3 cut(s)	697, 1290, 1301
BsiHKCI	CYCGRG	2 cut(s)	136, 1294
BsiSI	CCGG	9 cut(s)	314, 581, 645, 664, 867, 972, 991, 1016, 1206
BslFI	GGGAC	2 cut(s)	762, 804
BslI	CCNNNNNNNGG	6 cut(s)	688, 689, 785, 786, 1128, 1435
BsmAI	GTCTC	4 cut(s)	33, 981, 1381, 1400
BsmBI	CGTCTC	1 cut(s)	33
BsmFI	GGGAC	2 cut(s)	762, 804
BsmI	GAATGC	2 cut(s)	931, 958
BsnI	GGCC	5 cut(s)	317, 506, 644, 1019, 1418
Bso31I	GGTCTC	3 cut(s)	981, 1381, 1400
BsoBI	CYCGRG	2 cut(s)	136, 1294
Bsp119I	TTCGAA	2 cut(s)	624, 950
Bsp1286I	GDGCHC	5 cut(s)	117, 455, 697, 1290, 1301
Bsp143I	GATC	1 cut(s)	1455
Bsp1720I	GCTNAGC	5 cut(s)	651, 669, 696, 1307, 1497
Bsp19I	CCATGG	1 cut(s)	687
BspACI	CCGC	9 cut(s)	18, 24, 36, 294, 341, 721, 1034, 1042, 1045
BspANI	GGCC	5 cut(s)	317, 506, 644, 1019, 1418
BspCNI	CTCAG	7 cut(s)	219, 535, 555, 664, 709, 1333, 1489
BspHI	TCATGA	1 cut(s)	1567
BspLI	GGNNCC	4 cut(s)	22, 454, 777, 778
BspQI	GCTCTTC	1 cut(s)	1278
BspT104I	TTCGAA	2 cut(s)	624, 950
BspT107I	GGYRCC	1 cut(s)	20
BspTNI	GGTCTC	3 cut(s)	981, 1381, 1400
BsrBI	CCGCTC	1 cut(s)	18
BsrDI	GCAATG	1 cut(s)	1508
BsrFI	RCCGGY	4 cut(s)	313, 644, 971, 1015
BsrI	ACTGG	4 cut(s)	191, 567, 801, 1516
BssAI	RCCGGY	4 cut(s)	313, 644, 971, 1015
BssECI	CCNNGG	4 cut(s)	427, 687, 779, 1419
BssMI	GATC	1 cut(s)	1455
BssNI	GRCGYC	3 cut(s)	21, 1118, 1440
BssT1I	CCWWGG	2 cut(s)	687, 1419
Bst2UI	CCWGG	3 cut(s)	428, 781, 1392
Bst4CI	ACNGT	3 cut(s)	905, 967, 1360
Bst6I	CTCTTC	3 cut(s)	244, 815, 1278
BstACI	GRCGYC	3 cut(s)	21, 1118, 1440
BstAPI	GCANNNNNTGC	1 cut(s)	355
BstBI	TTCGAA	2 cut(s)	624, 950
BstC8I	GCNNGC	5 cut(s)	315, 646, 1017, 1221, 1449
BstDSI	CCRYGG	1 cut(s)	687
BstF5I	GGATG	6 cut(s)	36, 865, 892, 1138, 1237, 1256
BstH2I	RGCGCY	4 cut(s)	24, 555, 651, 721
BstHHI	GCGC	4 cut(s)	23, 554, 650, 720
BstKTI	GATC	1 cut(s)	1458
BstMAI	GTCTC	4 cut(s)	33, 981, 1381, 1400
BstMBI	GATC	1 cut(s)	1455
BstMCI	CGRYCG	1 cut(s)	645
BstMWI	GCNNNNNNNGC	7 cut(s)	346, 355, 618, 627, 636, 645, 1075
BstNI	CCWGG	3 cut(s)	428, 781, 1392
BstNSI	RCATGY	2 cut(s)	1078, 1375
BstSCI	CCNGG	5 cut(s)	426, 663, 779, 1204, 1390
BstSFI	CTRYAG	1 cut(s)	732
BstV1I	GCAGC	5 cut(s)	65, 419, 642, 1203, 1448
BstV2I	GAAGAC	1 cut(s)	51
BstZI	CGGCCG	1 cut(s)	642
BsuRI	GGCC	5 cut(s)	317, 506, 644, 1019, 1418
BtgI	CCRYGG	1 cut(s)	687
BtgZI	GCGATG	1 cut(s)	753
BtsCI	GGATG	6 cut(s)	36, 865, 892, 1138, 1237, 1256
BtsIMutI	CAGTG	1 cut(s)	963
Cac8I	GCNNGC	5 cut(s)	315, 646, 1017, 1221, 1449
CaiI	CAGNNNCTG	1 cut(s)	658
CciI	TCATGA	1 cut(s)	1567
CfoI	GCGC	4 cut(s)	23, 554, 650, 720
Cfr10I	RCCGGY	4 cut(s)	313, 644, 971, 1015
Cfr13I	GGNCC	4 cut(s)	63, 505, 776, 1416
CseI	GACGC	4 cut(s)	45, 59, 446, 1107
CsiI	ACCWGGT	1 cut(s)	1390
Csp6I	GTAC	7 cut(s)	190, 531, 602, 784, 901, 1091, 1544
CspAI	ACCGGT	1 cut(s)	971
CviAII	CATG	7 cut(s)	193, 230, 688, 1075, 1372, 1411, 1568
CviQI	GTAC	7 cut(s)	190, 531, 602, 784, 901, 1091, 1544
DinI	GGCGCC	1 cut(s)	22
DpnI	GATC	1 cut(s)	1457
DpnII	GATC	1 cut(s)	1455
DriI	GACNNNNNGTC	1 cut(s)	55
EaeI	YGGCCR	2 cut(s)	642, 1017
EagI	CGGCCG	1 cut(s)	642
Eam1104I	CTCTTC	3 cut(s)	244, 815, 1278
Eam1105I	GACNNNNNGTC	1 cut(s)	55
EarI	CTCTTC	3 cut(s)	244, 815, 1278
EciI	GGCGGA	4 cut(s)	51, 283, 1049, 1060
Ecl136II	GAGCTC	2 cut(s)	1288, 1299
EclXI	CGGCCG	1 cut(s)	642
Eco130I	CCWWGG	2 cut(s)	687, 1419
Eco24I	GRGCYC	4 cut(s)	117, 455, 1290, 1301
Eco31I	GGTCTC	3 cut(s)	981, 1381, 1400
Eco47I	GGWCC	2 cut(s)	63, 776
Eco52I	CGGCCG	1 cut(s)	642
Eco53kI	GAGCTC	2 cut(s)	1288, 1299
Eco88I	CYCGRG	2 cut(s)	136, 1294
EcoICRI	GAGCTC	2 cut(s)	1288, 1299
EcoO109I	RGGNCCY	2 cut(s)	776, 1416
EcoRII	CCWGG	3 cut(s)	426, 779, 1390
EcoT14I	CCWWGG	2 cut(s)	687, 1419
EcoT38I	GRGCYC	4 cut(s)	117, 455, 1290, 1301
EgeI	GGCGCC	1 cut(s)	22
EheI	GGCGCC	1 cut(s)	22
ErhI	CCWWGG	2 cut(s)	687, 1419
Esp3I	CGTCTC	1 cut(s)	33
FaeI	CATG	7 cut(s)	196, 233, 691, 1078, 1375, 1414, 1571
FalI	AAGNNNNNCTT	2 cut(s)	1530, 1562
FaqI	GGGAC	2 cut(s)	762, 804
FatI	CATG	7 cut(s)	192, 229, 687, 1074, 1371, 1410, 1567
FbaI	TGATCA	1 cut(s)	1455
FokI	GGATG	6 cut(s)	43, 872, 899, 1145, 1224, 1243
FriOI	GRGCYC	4 cut(s)	117, 455, 1290, 1301
FspBI	CTAG	1 cut(s)	1127
GlaI	GCGC	4 cut(s)	22, 553, 649, 719
GsaI	CCCAGC	2 cut(s)	218, 444
GsuI	CTGGAG	2 cut(s)	818, 1211
HaeII	RGCGCY	4 cut(s)	24, 555, 651, 721
HaeIII	GGCC	5 cut(s)	317, 506, 644, 1019, 1418
HapII	CCGG	9 cut(s)	314, 581, 645, 664, 867, 972, 991, 1016, 1206
HgaI	GACGC	4 cut(s)	45, 59, 446, 1107
HhaI	GCGC	4 cut(s)	23, 554, 650, 720
Hin1I	GRCGYC	3 cut(s)	21, 1118, 1440
Hin1II	CATG	7 cut(s)	196, 233, 691, 1078, 1375, 1414, 1571
Hin6I	GCGC	4 cut(s)	21, 552, 648, 718
HinP1I	GCGC	4 cut(s)	21, 552, 648, 718
HincII	GTYRAC	1 cut(s)	1245
HindII	GTYRAC	1 cut(s)	1245
HindIII	AAGCTT	1 cut(s)	249
HinfI	GANTC	6 cut(s)	134, 287, 853, 881, 1193, 1553
HpaII	CCGG	9 cut(s)	314, 581, 645, 664, 867, 972, 991, 1016, 1206
HphI	GGTGA	4 cut(s)	194, 758, 848, 961
Hpy166II	GTNNAC	1 cut(s)	1245
Hpy188I	TCNGA	7 cut(s)	9, 256, 492, 525, 886, 1386, 1493
Hpy188III	TCNNGA	6 cut(s)	221, 326, 536, 712, 857, 1568
Hpy8I	GTNNAC	1 cut(s)	1245
Hpy99I	CGWCG	5 cut(s)	53, 313, 395, 440, 611
HpyCH4III	ACNGT	3 cut(s)	905, 967, 1360
HpyCH4IV	ACGT	3 cut(s)	201, 390, 1440
HpyCH4V	TGCA	3 cut(s)	277, 1136, 1506
HpyF10VI	GCNNNNNNNGC	7 cut(s)	346, 355, 618, 627, 636, 645, 1075
HpySE526I	ACGT	3 cut(s)	201, 390, 1440
Hsp92I	GRCGYC	3 cut(s)	21, 1118, 1440
Hsp92II	CATG	7 cut(s)	196, 233, 691, 1078, 1375, 1414, 1571
HspAI	GCGC	4 cut(s)	21, 552, 648, 718
KasI	GGCGCC	1 cut(s)	20
KflI	GGGWCCC	1 cut(s)	776
KroI	GCCGGC	3 cut(s)	313, 644, 1015
KroNI	GCCGGC	3 cut(s)	315, 646, 1017
Ksp22I	TGATCA	1 cut(s)	1455
Kzo9I	GATC	1 cut(s)	1455
LguI	GCTCTTC	1 cut(s)	1278
LmnI	GCTCC	7 cut(s)	146, 274, 404, 458, 678, 1204, 1304
Lsp1109I	GCAGC	5 cut(s)	65, 419, 642, 1203, 1448
LweI	GCATC	1 cut(s)	1123
MabI	ACCWGGT	1 cut(s)	1390
MaeI	CTAG	1 cut(s)	1127
MaeII	ACGT	3 cut(s)	201, 390, 1440
MaeIII	GTNAC	3 cut(s)	182, 583, 967
MalI	GATC	1 cut(s)	1457
MbiI	CCGCTC	1 cut(s)	18
MboI	GATC	1 cut(s)	1455
MboII	GAAGA	9 cut(s)	56, 132, 231, 823, 832, 943, 1015, 1063, 1295
MfeI	CAATTG	1 cut(s)	1137
MhlI	GDGCHC	5 cut(s)	117, 455, 697, 1290, 1301
MluCI	AATT	8 cut(s)	478, 511, 752, 760, 874, 946, 1024, 1137
Mly113I	GGCGCC	1 cut(s)	21
MlyI	GAGTC	5 cut(s)	128, 281, 862, 875, 1202
MmeI	TCCRAC	1 cut(s)	426
MroNI	GCCGGC	3 cut(s)	313, 644, 1015
MroXI	GAANNNNTTC	1 cut(s)	946
MseI	TTAA	3 cut(s)	1170, 1278, 1362
MspA1I	CMGCKG	3 cut(s)	655, 1461, 1559
MspI	CCGG	9 cut(s)	314, 581, 645, 664, 867, 972, 991, 1016, 1206
MspR9I	CCNGG	5 cut(s)	428, 665, 781, 1206, 1392
MunI	CAATTG	1 cut(s)	1137
Mva1269I	GAATGC	2 cut(s)	931, 958
MvaI	CCWGG	3 cut(s)	428, 781, 1392
MwoI	GCNNNNNNNGC	7 cut(s)	346, 355, 618, 627, 636, 645, 1075
NaeI	GCCGGC	3 cut(s)	315, 646, 1017
NarI	GGCGCC	1 cut(s)	21
NciI	CCSGG	2 cut(s)	665, 1206
NcoI	CCATGG	1 cut(s)	687
NdeII	GATC	1 cut(s)	1455
NgoMIV	GCCGGC	3 cut(s)	313, 644, 1015
NlaIII	CATG	7 cut(s)	196, 233, 691, 1078, 1375, 1414, 1571
NlaIV	GGNNCC	4 cut(s)	22, 454, 777, 778
NmeAIII	GCCGAG	2 cut(s)	580, 620
NmuCI	GTSAC	2 cut(s)	182, 967
NspI	RCATGY	2 cut(s)	1078, 1375
NspV	TTCGAA	2 cut(s)	624, 950
PaeR7I	CTCGAG	1 cut(s)	136
PagI	TCATGA	1 cut(s)	1567
PciI	ACATGT	1 cut(s)	1371
PciSI	GCTCTTC	1 cut(s)	1278
PcsI	WCGNNNNNNNCGW	1 cut(s)	630
PctI	GAATGC	2 cut(s)	931, 958
PdiI	GCCGGC	3 cut(s)	315, 646, 1017
PdmI	GAANNNNTTC	1 cut(s)	946
PfeI	GAWTC	1 cut(s)	1553
PflFI	GACNNNGTC	1 cut(s)	1392
PinAI	ACCGGT	1 cut(s)	971
PleI	GAGTC	5 cut(s)	128, 281, 861, 875, 1201
PluTI	GGCGCC	1 cut(s)	24
PpsI	GAGTC	5 cut(s)	128, 281, 861, 875, 1201
PpuMI	RGGWCCY	1 cut(s)	776
PscI	ACATGT	1 cut(s)	1371
PshBI	ATTAAT	1 cut(s)	1170
Psp124BI	GAGCTC	2 cut(s)	1290, 1301
Psp1406I	AACGTT	1 cut(s)	201
Psp5II	RGGWCCY	1 cut(s)	776
Psp6I	CCWGG	3 cut(s)	426, 779, 1390
PspFI	CCCAGC	2 cut(s)	214, 440
PspGI	CCWGG	3 cut(s)	426, 779, 1390
PspN4I	GGNNCC	4 cut(s)	22, 454, 777, 778
PspPI	GGNCC	4 cut(s)	63, 505, 776, 1416
PspPPI	RGGWCCY	1 cut(s)	776
PspXI	VCTCGAGB	1 cut(s)	136
PstNI	CAGNNNCTG	1 cut(s)	658
PsyI	GACNNNGTC	1 cut(s)	1392
PvuII	CAGCTG	3 cut(s)	655, 1461, 1559
RsaI	GTAC	7 cut(s)	191, 532, 603, 785, 902, 1092, 1545
RsaNI	GTAC	7 cut(s)	190, 531, 602, 784, 901, 1091, 1544
SacI	GAGCTC	2 cut(s)	1290, 1301
SapI	GCTCTTC	1 cut(s)	1278
SaqAI	TTAA	3 cut(s)	1170, 1278, 1362
Sau3AI	GATC	1 cut(s)	1455
Sau96I	GGNCC	4 cut(s)	63, 505, 776, 1416
ScaI	AGTACT	1 cut(s)	1545
SchI	GAGTC	5 cut(s)	128, 281, 862, 875, 1202
ScrFI	CCNGG	5 cut(s)	428, 665, 781, 1206, 1392
SduI	GDGCHC	5 cut(s)	117, 455, 697, 1290, 1301
SexAI	ACCWGGT	1 cut(s)	1390
SfaNI	GCATC	1 cut(s)	1123
SfcI	CTRYAG	1 cut(s)	732
SfoI	GGCGCC	1 cut(s)	22
Sfr274I	CTCGAG	1 cut(s)	136
SfuI	TTCGAA	2 cut(s)	624, 950
SgrAI	CRCCGGYG	1 cut(s)	971
SinI	GGWCC	2 cut(s)	63, 776
SlaI	CTCGAG	1 cut(s)	136
SmlI	CTYRAG	2 cut(s)	136, 219
SmoI	CTYRAG	2 cut(s)	136, 219
Sse9I	AATT	8 cut(s)	478, 511, 752, 760, 874, 946, 1024, 1137
SsiI	CCGC	9 cut(s)	18, 24, 36, 294, 341, 721, 1034, 1042, 1045
SspDI	GGCGCC	1 cut(s)	20
SspMI	CTAG	1 cut(s)	1127
SstI	GAGCTC	2 cut(s)	1290, 1301
StyD4I	CCNGG	5 cut(s)	426, 663, 779, 1204, 1390
StyI	CCWWGG	2 cut(s)	687, 1419
TaaI	ACNGT	3 cut(s)	905, 967, 1360
TaiI	ACGT	3 cut(s)	204, 393, 1443
TasI	AATT	8 cut(s)	478, 511, 752, 760, 874, 946, 1024, 1137
TatI	WGTACW	3 cut(s)	601, 900, 1543
TauI	GCSGC	5 cut(s)	21, 26, 343, 723, 1045
TfiI	GAWTC	1 cut(s)	1553
Tru1I	TTAA	3 cut(s)	1170, 1278, 1362
Tru9I	TTAA	3 cut(s)	1170, 1278, 1362
TscAI	CASTG	1 cut(s)	970
TseFI	GTSAC	2 cut(s)	182, 967
TseI	GCWGC	5 cut(s)	53, 432, 655, 1216, 1461
Tsp45I	GTSAC	2 cut(s)	182, 967
TspDTI	ATGAA	3 cut(s)	209, 1226, 1556
TspGWI	ACGGA	3 cut(s)	297, 602, 1230
TspRI	CASTG	1 cut(s)	970
Tth111I	GACNNNGTC	1 cut(s)	1392
VpaK11BI	GGWCC	2 cut(s)	63, 776
VspI	ATTAAT	1 cut(s)	1170
XapI	RAATTY	2 cut(s)	760, 1024
XceI	RCATGY	2 cut(s)	1078, 1375
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1027, 1417
XhoI	CTCGAG	1 cut(s)	136
XmnI	GAANNNNTTC	1 cut(s)	946
XspI	CTAG	1 cut(s)	1127
ZraI	GACGTC	1 cut(s)	1441
ZrmI	AGTACT	1 cut(s)	1545

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.