Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001985

Physical Location & Seq

Genomic Coordinates Reverse (-)

24129317 .. 24132300

Gene Structure 2984 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1148600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGCTGCCAAGGCTGAACGGTGGTGTTTGGATGGACGACAGGGAGGACAGAGACTCTGTTTCCTGGACTAGAACCTCCGCCACCGCCACCGCCGGCAACACCGAGAACAAGGACGAGATGGGTTCTTCTCTGTCTACCTTCAAATCGATGCTGGAGGTTGAAGACGACTGGTACATGGCGGCGAATAACAGCATCCAGGGCCATTCAGATGTCGGAGACATCAGCTTCTCGCCGAGCTTTGCCGACCCAGAAAGCTTGCTGCTCAACCCGGTGGACTCTTCCTCCTCCTGCTCACCGTCGTCGTCGGTTTTCAACAATCTCGACCCGAATCAGGTTCATTACTACATGCCTCAAAATCCCAACTTGTCTTCGCTGCTTAATGTTGTTCCTAACAACCCTTTGGATCACGGCTTCGATTTGGGCTGTGACATTGGGTTTCTTGACACTCAAGCTTCGGGTGGCGGTTCGAGTATGATGAACAGGGGAGGTGGGGTTTTATCTGGATTCAATGATTTAAGCTCGAATAGCCAGGTGAATGCTCAGAATTTGGGTTCTAATTTACAGTTTTCGACTACCCGCATGCCTCAAGCTCTTGAAAACAGTTCAAATTTTTCGGGTTTTCGAGGTGTGGATGATGGTTCGGCAAATGCTTTGTTTCCCAATAGGCCTAAGTTACTGAGACCACTTGAATCCTTCCCTTCTGTGGGAGTCCAGCCAACTCTTTTCCAGAAAAGAGCAGCGCTTCGGAAAAACTTAGGCGATGGTGGAGGGAATTTGGGTGTTTTGGTCTCACAAGGGGGCCTGGTTTTGAATGAAGGGGATGAGAGGAAGAGGGAGATGGGTGTGCAGAATGAGAAGAAGAGGAAAATGAGCGGCGGAGATGATGTCGATGATCTGAGTTTCGATGGTTCGGGTTTGAACTATGATTCGGATGAGTTTACTGAGAACACTAAGGTTGATGACGGTGCCAAGAATGGTGGGAACAGCTCGAATGCAAATAGCACGGTTACCGGTGGAGGAGGGGGTCATAAGGGGAAGAAGAAAGGGTTGCCTGCCAAGAATCTGATGGCTGAGAGGCGCCGCCGGAAGAAGCTCAATGATAGGCTGTACATGCTGAGATCCGTTGTTCCGAAGATTAGCAAAATGGACAGGGCCTCAATCCTTGGGGATGCAATTGAGTACTTGAAGGAACTTCTGCAGAGGATCAACAACCTCCACAATGAACTGGAGTCAATCCCTCCTGGATCTGCATTGACACCTACCGGAAATACTTTCCACCCTTTGACACCCACTCCGGCTACTCTGCCAAATCGTATCAAGGAAGAACTTTGCCCCAGCTCATTACCTAGCCCAAATGGCCAAGCTGCAAGGGTTGAAGTTAGGCTGCGAGAAGGACGAGCAGTTAACATCCACATGTTCTGCGGCCGAAGGCCAGGTCTCTTGCTCTCAACCATGAGAACTTTGGATAACCTTGGGTTAGACATCCAGCAAGCCGTCATCAGCTGTTTCAATGGTTTTGCTATGGATGTCTTCCGTGCCGAGCAATGCAAGGAAGGCCAAGACGTCCACCCGGATCAGATAAAGGCGGTACTTCTGGATTCGATCGGGTTCCATGGCATGATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

542

Amino Acids

58.49

Weight (kDa)

5.6

Isoelectric Point (pI)

48.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH_SAC51	PF23173	355 - 404	2.6e-07	Transcription factor SAC51 bHLH domain
HLH	PF00010	356 - 398	2.5e-09	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	458 - 533	7.8e-12	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12860 AT1G12860 AT1G12860 AT3G26744 AT3G26744 AT3G26744 AT3G26744 AT3G26744
fragaria_vesca	FvH4_5g09300 FvH4_5g09300
malus_domestica	MD06G1132200.v1.1 MD09G1003800.v1.1 MD14G1148600.v1.1 MD17G1007400.v1.1
prunus_persica	Prupe.3G312800_v2.0.a1 Prupe.3G312800_v2.0.a1 Prupe.5G143200_v2.0.a1
pyrus_communis	pycom06g12380 pycom111g00280 pycom14g12340 pycom17g00300
rosa_chinensis	RchiOBHm_Chr2g0176421 RchiOBHm_Chr7g0188921
rosa_laevigata	RLG00000004651 RLG00000022412
rosa_multiflora	Rmu_co8326225.1_g000001 Rmu_co8487291.1_g000001 Rmu_sc0009140.1_g000010 Rmu_sc0011904.1_g000015 Rmu_ssc0000113.1_g000008
rosa_roxburghii	Rroxscaffold_2G00076450 Rroxscaffold_3G00265940
rosa_rugosa	Rorug02G0594200 Rorug02G0594200 Rorug06G0494600
rosa_samantha	Rh2AG672700 Rh2BG684200 Rh2CG647000 Rh2DG697800 Rh7AG099500 Rh7BG101600 Rh7CG102900 Rh7DG101300
rosa_wichuraiana	Rw2G055180 Rw7G008570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1566
AccB1I	GGYRCC	2 cut(s)	965, 1077
AccBSI	CCGCTC	1 cut(s)	873
AccI	GTMKAC	1 cut(s)	134
AclWI	GGATC	5 cut(s)	411, 1113, 1211, 1252, 1581
AcoI	YGGCCR	2 cut(s)	1357, 1423
AcsI	RAATTY	3 cut(s)	544, 607, 772
AcyI	GRCGYC	2 cut(s)	1078, 1563
AfaI	GTAC	4 cut(s)	173, 1109, 1181, 1590
AfeI	AGCGCT	1 cut(s)	741
AfiI	CCNNNNNNNGG	3 cut(s)	331, 703, 704
AflIII	ACRYGT	1 cut(s)	1413
AgeI	ACCGGT	1 cut(s)	1010
AjnI	CCWGG	6 cut(s)	62, 195, 528, 801, 1240, 1432
Alw26I	GTCTC	5 cut(s)	45, 210, 673, 793, 1442
AlwI	GGATC	5 cut(s)	411, 1113, 1211, 1252, 1581
Aor51HI	AGCGCT	1 cut(s)	741
AoxI	GGCC	8 cut(s)	199, 665, 799, 1152, 1357, 1423, 1430, 1555
ApeKI	GCWGC	6 cut(s)	4, 259, 373, 737, 1364, 1384
ApoI	RAATTY	3 cut(s)	544, 607, 772
ArsI	GACNNNNNNTTYG	2 cut(s)	1420, 1452
AsiGI	ACCGGT	1 cut(s)	1010
AspLEI	GCGC	2 cut(s)	742, 1080
AspS9I	GGNCC	3 cut(s)	199, 799, 1152
AsuC2I	CCSGG	2 cut(s)	269, 1571
AsuHPI	GGTGA	2 cut(s)	285, 544
BalI	TGGCCA	1 cut(s)	1359
BanI	GGYRCC	2 cut(s)	965, 1077
BbsI	GAAGAC	3 cut(s)	168, 360, 1522
BbvI	GCAGC	5 cut(s)	246, 360, 749, 1351, 1371
BccI	CCATC	7 cut(s)	25, 112, 629, 755, 832, 899, 1060
BceAI	ACGGC	2 cut(s)	424, 1478
BciT130I	CCWGG	6 cut(s)	64, 197, 530, 803, 1242, 1434
BcnI	CCSGG	2 cut(s)	269, 1571
BcoDI	GTCTC	5 cut(s)	45, 210, 673, 793, 1442
BfaI	CTAG	2 cut(s)	69, 1347
BfmI	CTRYAG	1 cut(s)	1196
BfoI	RGCGCY	2 cut(s)	743, 1081
BglI	GCCNNNNNGGC	1 cut(s)	1356
BmcAI	AGTACT	1 cut(s)	1181
Bme1390I	CCNGG	8 cut(s)	64, 197, 269, 530, 803, 1242, 1434, 1571
BmgT120I	GGNCC	3 cut(s)	199, 799, 1152
BmiI	GGNNCC	4 cut(s)	800, 967, 1079, 1610
BmrFI	CCNGG	8 cut(s)	64, 197, 269, 530, 803, 1242, 1434, 1571
BmsI	GCATC	3 cut(s)	138, 201, 1159
BpiI	GAAGAC	3 cut(s)	168, 360, 1522
BpmI	CTGGAG	2 cut(s)	173, 1247
BpuEI	CTTGAG	2 cut(s)	432, 570
BpuMI	CCSGG	2 cut(s)	269, 1571
Bsa29I	ATCGAT	1 cut(s)	146
BsaHI	GRCGYC	2 cut(s)	1078, 1563
BsaI	GGTCTC	3 cut(s)	673, 793, 1442
BsaJI	CCNNGG	5 cut(s)	8, 196, 1162, 1471, 1612
BsaWI	WCCGGW	2 cut(s)	1010, 1262
BsaXI	ACNNNNNCTCC	8 cut(s)	266, 296, 477, 507, 870, 900, 1276, 1306
Bsc4I	CCNNNNNNNGG	3 cut(s)	331, 703, 704
Bse118I	RCCGGY	2 cut(s)	92, 1010
Bse1I	ACTGG	2 cut(s)	173, 1230
Bse3DI	GCAATG	1 cut(s)	1550
BseBI	CCWGG	6 cut(s)	64, 197, 530, 803, 1242, 1434
BseCI	ATCGAT	1 cut(s)	146
BseDI	CCNNGG	5 cut(s)	8, 196, 1162, 1471, 1612
BseGI	GGATG	9 cut(s)	36, 192, 637, 826, 937, 1174, 1407, 1482, 1531
BseLI	CCNNNNNNNGG	3 cut(s)	331, 703, 704
BseMI	GCAATG	1 cut(s)	1550
BseMII	CTCAG	6 cut(s)	554, 668, 887, 933, 1062, 1106
BseNI	ACTGG	2 cut(s)	173, 1230
BseRI	GAGGAG	2 cut(s)	274, 1032
BseX3I	CGGCCG	1 cut(s)	1423
BseXI	GCAGC	5 cut(s)	246, 360, 749, 1351, 1371
BseYI	CCCAGC	1 cut(s)	1334
BsgI	GTGCAG	1 cut(s)	866
Bsh1285I	CGRYCG	2 cut(s)	1426, 1605
BshFI	GGCC	8 cut(s)	201, 667, 801, 1154, 1359, 1425, 1432, 1557
BshNI	GGYRCC	2 cut(s)	965, 1077
BshTI	ACCGGT	1 cut(s)	1010
BshVI	ATCGAT	1 cut(s)	146
BsiEI	CGRYCG	2 cut(s)	1426, 1605
BsiSI	CCGG	7 cut(s)	93, 269, 1011, 1084, 1263, 1295, 1571
BslI	CCNNNNNNNGG	3 cut(s)	331, 703, 704
BsmAI	GTCTC	5 cut(s)	45, 210, 673, 793, 1442
BsmI	GAATGC	2 cut(s)	541, 997
BsnI	GGCC	8 cut(s)	201, 667, 801, 1154, 1359, 1425, 1432, 1557
Bso31I	GGTCTC	3 cut(s)	673, 793, 1442
Bsp1407I	TGTACA	1 cut(s)	1107
Bsp143I	GATC	7 cut(s)	403, 892, 1118, 1203, 1244, 1573, 1602
Bsp19I	CCATGG	1 cut(s)	1612
BspANI	GGCC	8 cut(s)	201, 667, 801, 1154, 1359, 1425, 1432, 1557
BspCNI	CTCAG	6 cut(s)	553, 669, 888, 934, 1063, 1107
BspDI	ATCGAT	1 cut(s)	146
BspLI	GGNNCC	4 cut(s)	800, 967, 1079, 1610
BspMAI	CTGCAG	1 cut(s)	1200
BspPI	GGATC	5 cut(s)	411, 1113, 1211, 1252, 1581
BspT107I	GGYRCC	2 cut(s)	965, 1077
BspTNI	GGTCTC	3 cut(s)	673, 793, 1442
BsrBI	CCGCTC	1 cut(s)	873
BsrDI	GCAATG	1 cut(s)	1550
BsrFI	RCCGGY	2 cut(s)	92, 1010
BsrGI	TGTACA	1 cut(s)	1107
BsrI	ACTGG	2 cut(s)	173, 1230
BssAI	RCCGGY	2 cut(s)	92, 1010
BssECI	CCNNGG	5 cut(s)	8, 196, 1162, 1471, 1612
BssMI	GATC	7 cut(s)	403, 892, 1118, 1203, 1244, 1573, 1602
BssNI	GRCGYC	2 cut(s)	1078, 1563
BssT1I	CCWWGG	4 cut(s)	8, 1162, 1471, 1612
Bst2UI	CCWGG	6 cut(s)	64, 197, 530, 803, 1242, 1434
Bst4CI	ACNGT	6 cut(s)	20, 297, 564, 602, 965, 1006
Bst6I	CTCTTC	3 cut(s)	283, 824, 854
BstACI	GRCGYC	2 cut(s)	1078, 1563
BstAUI	TGTACA	1 cut(s)	1107
BstC8I	GCNNGC	5 cut(s)	94, 257, 581, 1053, 1491
BstDEI	CTNAG	9 cut(s)	540, 669, 677, 754, 896, 942, 951, 1071, 1115
BstDSI	CCRYGG	1 cut(s)	1612
BstEII	GGTNACC	1 cut(s)	1006
BstF5I	GGATG	9 cut(s)	36, 192, 637, 826, 937, 1174, 1407, 1482, 1531
BstH2I	RGCGCY	2 cut(s)	743, 1081
BstHHI	GCGC	2 cut(s)	742, 1080
BstKTI	GATC	7 cut(s)	406, 895, 1121, 1206, 1247, 1576, 1605
BstMAI	GTCTC	5 cut(s)	45, 210, 673, 793, 1442
BstMBI	GATC	7 cut(s)	403, 892, 1118, 1203, 1244, 1573, 1602
BstMCI	CGRYCG	2 cut(s)	1426, 1605
BstMWI	GCNNNNNNNGC	6 cut(s)	10, 198, 525, 1111, 1356, 1554
BstNI	CCWGG	6 cut(s)	64, 197, 530, 803, 1242, 1434
BstNSI	RCATGY	4 cut(s)	349, 583, 1114, 1417
BstPI	GGTNACC	1 cut(s)	1006
BstSCI	CCNGG	8 cut(s)	62, 195, 267, 528, 801, 1240, 1432, 1569
BstSFI	CTRYAG	1 cut(s)	1196
BstV1I	GCAGC	5 cut(s)	246, 360, 749, 1351, 1371
BstV2I	GAAGAC	3 cut(s)	168, 360, 1522
BstX2I	RGATCY	2 cut(s)	1118, 1244
BstYI	RGATCY	2 cut(s)	1118, 1244
BstZI	CGGCCG	1 cut(s)	1423
Bsu15I	ATCGAT	1 cut(s)	146
BsuRI	GGCC	8 cut(s)	201, 667, 801, 1154, 1359, 1425, 1432, 1557
BsuTUI	ATCGAT	1 cut(s)	146
BtgI	CCRYGG	1 cut(s)	1612
BtgZI	GCGATG	1 cut(s)	774
BtsCI	GGATG	9 cut(s)	36, 192, 637, 826, 937, 1174, 1407, 1482, 1531
Cac8I	GCNNGC	5 cut(s)	94, 257, 581, 1053, 1491
CfoI	GCGC	2 cut(s)	742, 1080
Cfr10I	RCCGGY	2 cut(s)	92, 1010
Cfr13I	GGNCC	3 cut(s)	199, 799, 1152
ClaI	ATCGAT	1 cut(s)	146
Csp6I	GTAC	4 cut(s)	172, 1108, 1180, 1589
CspAI	ACCGGT	1 cut(s)	1010
CspCI	CAANNNNNGTGG	2 cut(s)	958, 993
CviAII	CATG	8 cut(s)	175, 346, 580, 1111, 1414, 1453, 1613, 1618
CviQI	GTAC	4 cut(s)	172, 1108, 1180, 1589
DdeI	CTNAG	9 cut(s)	540, 669, 677, 754, 896, 942, 951, 1071, 1115
DinI	GGCGCC	1 cut(s)	1079
DpnI	GATC	7 cut(s)	405, 894, 1120, 1205, 1246, 1575, 1604
DpnII	GATC	7 cut(s)	403, 892, 1118, 1203, 1244, 1573, 1602
EaeI	YGGCCR	2 cut(s)	1357, 1423
EagI	CGGCCG	1 cut(s)	1423
Eam1104I	CTCTTC	3 cut(s)	283, 824, 854
EarI	CTCTTC	3 cut(s)	283, 824, 854
EciI	GGCGGA	2 cut(s)	67, 891
EclXI	CGGCCG	1 cut(s)	1423
Eco130I	CCWWGG	4 cut(s)	8, 1162, 1471, 1612
Eco147I	AGGCCT	1 cut(s)	667
Eco31I	GGTCTC	3 cut(s)	673, 793, 1442
Eco47III	AGCGCT	1 cut(s)	741
Eco52I	CGGCCG	1 cut(s)	1423
Eco91I	GGTNACC	1 cut(s)	1006
EcoO109I	RGGNCCY	2 cut(s)	799, 1152
EcoO65I	GGTNACC	1 cut(s)	1006
EcoRII	CCWGG	6 cut(s)	62, 195, 528, 801, 1240, 1432
EcoT14I	CCWWGG	4 cut(s)	8, 1162, 1471, 1612
EgeI	GGCGCC	1 cut(s)	1079
EheI	GGCGCC	1 cut(s)	1079
ErhI	CCWWGG	4 cut(s)	8, 1162, 1471, 1612
FaeI	CATG	8 cut(s)	178, 349, 583, 1114, 1417, 1456, 1616, 1621
FatI	CATG	8 cut(s)	174, 345, 579, 1110, 1413, 1452, 1612, 1617
FauI	CCCGC	1 cut(s)	584
FblI	GTMKAC	1 cut(s)	134
FokI	GGATG	9 cut(s)	43, 179, 644, 833, 944, 1181, 1394, 1469, 1538
FspBI	CTAG	2 cut(s)	69, 1347
GlaI	GCGC	2 cut(s)	741, 1079
GsaI	CCCAGC	1 cut(s)	1338
GsuI	CTGGAG	2 cut(s)	173, 1247
HaeII	RGCGCY	2 cut(s)	743, 1081
HaeIII	GGCC	8 cut(s)	201, 667, 801, 1154, 1359, 1425, 1432, 1557
HapII	CCGG	7 cut(s)	93, 269, 1011, 1084, 1263, 1295, 1571
HhaI	GCGC	2 cut(s)	742, 1080
Hin1I	GRCGYC	2 cut(s)	1078, 1563
Hin1II	CATG	8 cut(s)	178, 349, 583, 1114, 1417, 1456, 1616, 1621
Hin6I	GCGC	2 cut(s)	740, 1078
HinP1I	GCGC	2 cut(s)	740, 1078
HincII	GTYRAC	1 cut(s)	1405
HindII	GTYRAC	1 cut(s)	1405
HindIII	AAGCTT	2 cut(s)	253, 450
HpaI	GTTAAC	1 cut(s)	1405
HpaII	CCGG	7 cut(s)	93, 269, 1011, 1084, 1263, 1295, 1571
HphI	GGTGA	2 cut(s)	285, 544
Hpy166II	GTNNAC	5 cut(s)	135, 274, 939, 1405, 1567
Hpy188I	TCNGA	9 cut(s)	208, 215, 543, 747, 897, 931, 1065, 1131, 1578
Hpy188III	TCNNGA	6 cut(s)	320, 440, 501, 593, 727, 1595
Hpy8I	GTNNAC	5 cut(s)	135, 274, 939, 1405, 1567
Hpy99I	CGWCG	3 cut(s)	301, 304, 307
HpyAV	CCTTC	8 cut(s)	148, 703, 708, 809, 1180, 1385, 1422, 1547
HpyCH4III	ACNGT	6 cut(s)	20, 297, 564, 602, 965, 1006
HpyCH4IV	ACGT	1 cut(s)	1563
HpyCH4V	TGCA	7 cut(s)	847, 995, 1172, 1198, 1250, 1367, 1548
HpyF10VI	GCNNNNNNNGC	6 cut(s)	10, 198, 525, 1111, 1356, 1554
HpyF3I	CTNAG	9 cut(s)	540, 669, 677, 754, 896, 942, 951, 1071, 1115
HpySE526I	ACGT	1 cut(s)	1563
Hsp92I	GRCGYC	2 cut(s)	1078, 1563
Hsp92II	CATG	8 cut(s)	178, 349, 583, 1114, 1417, 1456, 1616, 1621
HspAI	GCGC	2 cut(s)	740, 1078
KasI	GGCGCC	1 cut(s)	1077
KroI	GCCGGC	1 cut(s)	92
KroNI	GCCGGC	1 cut(s)	94
KspAI	GTTAAC	1 cut(s)	1405
Kzo9I	GATC	7 cut(s)	403, 892, 1118, 1203, 1244, 1573, 1602
Lsp1109I	GCAGC	5 cut(s)	246, 360, 749, 1351, 1371
LweI	GCATC	3 cut(s)	138, 201, 1159
MaeI	CTAG	2 cut(s)	69, 1347
MaeII	ACGT	1 cut(s)	1563
MaeIII	GTNAC	3 cut(s)	425, 672, 1006
MalI	GATC	7 cut(s)	405, 894, 1120, 1205, 1246, 1575, 1604
MbiI	CCGCTC	1 cut(s)	873
MboI	GATC	7 cut(s)	403, 892, 1118, 1203, 1244, 1573, 1602
MfeI	CAATTG	1 cut(s)	1173
MflI	RGATCY	2 cut(s)	1118, 1244
MlsI	TGGCCA	1 cut(s)	1359
MluCI	AATT	5 cut(s)	544, 556, 607, 772, 1173
MluNI	TGGCCA	1 cut(s)	1359
Mly113I	GGCGCC	1 cut(s)	1078
MlyI	GAGTC	4 cut(s)	47, 269, 717, 1238
MmeI	TCCRAC	1 cut(s)	193
Mox20I	TGGCCA	1 cut(s)	1359
MroNI	GCCGGC	1 cut(s)	92
MscI	TGGCCA	1 cut(s)	1359
MseI	TTAA	3 cut(s)	378, 515, 1404
MslI	CAYNNNNRTG	2 cut(s)	207, 1412
Msp20I	TGGCCA	1 cut(s)	1359
MspA1I	CMGCKG	1 cut(s)	1503
MspI	CCGG	7 cut(s)	93, 269, 1011, 1084, 1263, 1295, 1571
MspR9I	CCNGG	8 cut(s)	64, 197, 269, 530, 803, 1242, 1434, 1571
MunI	CAATTG	1 cut(s)	1173
Mva1269I	GAATGC	2 cut(s)	541, 997
MvaI	CCWGG	6 cut(s)	64, 197, 530, 803, 1242, 1434
MwoI	GCNNNNNNNGC	6 cut(s)	10, 198, 525, 1111, 1356, 1554
NaeI	GCCGGC	1 cut(s)	94
NarI	GGCGCC	1 cut(s)	1078
NciI	CCSGG	2 cut(s)	269, 1571
NcoI	CCATGG	1 cut(s)	1612
NdeII	GATC	7 cut(s)	403, 892, 1118, 1203, 1244, 1573, 1602
NgoMIV	GCCGGC	1 cut(s)	92
NlaIII	CATG	8 cut(s)	178, 349, 583, 1114, 1417, 1456, 1616, 1621
NlaIV	GGNNCC	4 cut(s)	800, 967, 1079, 1610
NmeAIII	GCCGAG	2 cut(s)	258, 1564
NmuCI	GTSAC	1 cut(s)	425
NspI	RCATGY	4 cut(s)	349, 583, 1114, 1417
PaeI	GCATGC	1 cut(s)	583
PceI	AGGCCT	1 cut(s)	667
PciI	ACATGT	1 cut(s)	1413
PctI	GAATGC	2 cut(s)	541, 997
PdiI	GCCGGC	1 cut(s)	94
PfeI	GAWTC	6 cut(s)	328, 504, 689, 926, 1060, 1598
PfoI	TCCNGGA	2 cut(s)	62, 1240
PinAI	ACCGGT	1 cut(s)	1010
Ple19I	CGATCG	1 cut(s)	1605
PleI	GAGTC	4 cut(s)	47, 269, 716, 1237
PluTI	GGCGCC	1 cut(s)	1081
PpsI	GAGTC	4 cut(s)	47, 269, 716, 1237
PscI	ACATGT	1 cut(s)	1413
Psp6I	CCWGG	6 cut(s)	62, 195, 528, 801, 1240, 1432
PspEI	GGTNACC	1 cut(s)	1006
PspFI	CCCAGC	1 cut(s)	1334
PspGI	CCWGG	6 cut(s)	62, 195, 528, 801, 1240, 1432
PspN4I	GGNNCC	4 cut(s)	800, 967, 1079, 1610
PspPI	GGNCC	3 cut(s)	199, 799, 1152
PstI	CTGCAG	1 cut(s)	1200
PsuI	RGATCY	2 cut(s)	1118, 1244
PvuI	CGATCG	1 cut(s)	1605
PvuII	CAGCTG	1 cut(s)	1503
RsaI	GTAC	4 cut(s)	173, 1109, 1181, 1590
RsaNI	GTAC	4 cut(s)	172, 1108, 1180, 1589
RseI	CAYNNNNRTG	2 cut(s)	207, 1412
SaqAI	TTAA	3 cut(s)	378, 515, 1404
Sau3AI	GATC	7 cut(s)	403, 892, 1118, 1203, 1244, 1573, 1602
Sau96I	GGNCC	3 cut(s)	199, 799, 1152
ScaI	AGTACT	1 cut(s)	1181
SchI	GAGTC	4 cut(s)	47, 269, 717, 1238
ScrFI	CCNGG	8 cut(s)	64, 197, 269, 530, 803, 1242, 1434, 1571
SfaNI	GCATC	3 cut(s)	138, 201, 1159
SfcI	CTRYAG	1 cut(s)	1196
SfoI	GGCGCC	1 cut(s)	1079
SmiMI	CAYNNNNRTG	2 cut(s)	207, 1412
SmlI	CTYRAG	2 cut(s)	447, 585
SmoI	CTYRAG	2 cut(s)	447, 585
SphI	GCATGC	1 cut(s)	583
Sse9I	AATT	5 cut(s)	544, 556, 607, 772, 1173
SseBI	AGGCCT	1 cut(s)	667
SspDI	GGCGCC	1 cut(s)	1077
SspMI	CTAG	2 cut(s)	69, 1347
StuI	AGGCCT	1 cut(s)	667
StyD4I	CCNGG	8 cut(s)	62, 195, 267, 528, 801, 1240, 1432, 1569
StyI	CCWWGG	4 cut(s)	8, 1162, 1471, 1612
TaaI	ACNGT	6 cut(s)	20, 297, 564, 602, 965, 1006
TaiI	ACGT	1 cut(s)	1566
TasI	AATT	5 cut(s)	544, 556, 607, 772, 1173
TatI	WGTACW	2 cut(s)	1107, 1179
TauI	GCSGC	4 cut(s)	182, 876, 1083, 1425
TfiI	GAWTC	6 cut(s)	328, 504, 689, 926, 1060, 1598
Tru1I	TTAA	3 cut(s)	378, 515, 1404
Tru9I	TTAA	3 cut(s)	378, 515, 1404
TseFI	GTSAC	1 cut(s)	425
TseI	GCWGC	6 cut(s)	4, 259, 373, 737, 1364, 1384
Tsp45I	GTSAC	1 cut(s)	425
TspDTI	ATGAA	4 cut(s)	326, 491, 828, 1236
TspGWI	ACGGA	2 cut(s)	1111, 1523
XapI	RAATTY	3 cut(s)	544, 607, 772
XceI	RCATGY	4 cut(s)	349, 583, 1114, 1417
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1063, 1459
XmiI	GTMKAC	1 cut(s)	134
XspI	CTAG	2 cut(s)	69, 1347
ZraI	GACGTC	1 cut(s)	1564
ZrmI	AGTACT	1 cut(s)	1181

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.