Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001985

Physical Location & Seq

Genomic Coordinates Reverse (-)

335088 .. 338411

Gene Structure 3324 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1003800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1581 bp

ATGCTTCCGATGTCGAGCGGCGCCGCTTGGATGGGCGGAGACGAAGACGACGCAGCGTCTTGGACCAGAAACAGCACCACCACCCACAACAACAACAACAACGAGGCAGAGCCCAGAAGAAACGACCAAGACTCGAGCTTGGGAGCTTCTTTTTCAAACTTCAAATCAATGCTGGAAGGTGACTGGTACATGAACAACGTTCTCAGCAACCCAGCTCAAGACCTCCATGCCTTCTCTTCCACCCAAGCTTCTGAAACTACACTTGCTCCTCTGCAACCAATCGACTCCTCCGCCTCCTGTTCTCCGTCGCCGGCCTTCTCTCTCGACCCTTCACAGCCGCCACAGCAGTTTTTGCCTCCAAAATCCTGCTTCTCCTCTCTCCTCAACGTCGTCTGCTCCAACCCTTTTGATAACAGCTTCGACCTGGGCTGCGACGCTGGGTTTCTGGGCTCCTTTCAGGGAAATCAGCCTTCCAATTCCTCTGTTCTGATGGGTTTCACGGCCCTCAATTCTCATGCTCAAATGGGTACTCCCGAACTCAGTTCGAGCGCCGAGTTTCCAGCCAGTCGGTTGCTTCCGGTAACGGACAACGCCAATGTACTCGACGGCGACTTTGGCTTCGAAGGCTTCGATGGCTCGGCCGGCGCTCAGCTGCTGAACCGGGCTAAGCTCCTGTTCCCTCCCATGGGTGCTCAGCCCACGCTTTTCCAGAAGCGCCGCCAAAACTCTGTAGGCGATGGGGGTGATAAATTGGGAAATTTGGAGATTTCGGGTCCCAGGTACGGAGGACTACTGGAGAGTTTGGAGAAGAAAAGGAAGAGGAACGAGGAGGGTGAGATGGAGGAGGGGAGTCTGGATGTTTCCGGTTTGAATTATGACTCGGATGACTTCAATGAGTACAGTCAAGTGGAGGTGGAGGAGAATGCGAAGAATGGTGGAAGCAATTCGAATGCCAACAGCACTGTCACCGGTGTAGAGGGAGGAGACCGGAAGGGCAAGAAGAAGGGTTTGCCGGCCAAAAATTTGATGGCGGAGAGGCGGCGGAGGAAGAAGCTCAATGATAGGCTCTACATGCTTAGGTCTGTTGTACCCAAGATAAGCAAGATGGATAGGGCGTCCATACTAGGGGATGCAATTGATTATTTGAAAGAGCTTCTACAAAGGATTAATGACCTCCATAACGAGCTGGAGTCAGCTCCACCCGGATCTTTGCTGCCTGCTTCAACAAGTTTTCATCCGTTGACACCCACTCCATCCACCCTTCCCTGCCGTGTTAAAGAAGAGCTCTGCCCAAGCTCCTTGCTAAGCCCCAAAACTCAGCCGAAGGTGGAGGTTCGGGTAAGGGAAGGGCGAACTGTTAATATCCACATGTTCTGTTCTCGGAGACCAGGTCTCTTGCTCTCTACCATGAGGGCCTTGGATAACCTTGATTTGGACGTCCAGCAGGCTGTGATCAGCTGCTTCAATGGGTTTGCTTTAGATGTGTTCCGAGCTGAGCAATGCAGGGAAAACCAGTTCTTGCCAGAGCAAATAAAAGCAGTACTTTTGGATTCAGCTGGATTTCATGATATGATGATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

527

Amino Acids

57.34

Weight (kDa)

5.09

Isoelectric Point (pI)

53.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH_SAC51	PF23173	342 - 390	9.9e-08	Transcription factor SAC51 bHLH domain
HLH	PF00010	343 - 385	1.9e-09	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	443 - 512	2.3e-12	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12860 AT1G12860 AT1G12860 AT3G26744 AT3G26744 AT3G26744 AT3G26744 AT3G26744
fragaria_vesca	FvH4_5g09300 FvH4_5g09300
malus_domestica	MD06G1132200.v1.1 MD09G1003800.v1.1 MD14G1148600.v1.1 MD17G1007400.v1.1
prunus_persica	Prupe.3G312800_v2.0.a1 Prupe.3G312800_v2.0.a1 Prupe.5G143200_v2.0.a1
pyrus_communis	pycom06g12380 pycom111g00280 pycom14g12340 pycom17g00300
rosa_chinensis	RchiOBHm_Chr2g0176421 RchiOBHm_Chr7g0188921
rosa_laevigata	RLG00000004651 RLG00000022412
rosa_multiflora	Rmu_co8326225.1_g000001 Rmu_co8487291.1_g000001 Rmu_sc0009140.1_g000010 Rmu_sc0011904.1_g000015 Rmu_ssc0000113.1_g000008
rosa_roxburghii	Rroxscaffold_2G00076450 Rroxscaffold_3G00265940
rosa_rugosa	Rorug02G0594200 Rorug02G0594200 Rorug06G0494600
rosa_samantha	Rh2AG672700 Rh2BG684200 Rh2CG647000 Rh2DG697800 Rh7AG099500 Rh7BG101600 Rh7CG102900 Rh7DG101300
rosa_wichuraiana	Rw2G055180 Rw7G008570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1440
AccB1I	GGYRCC	1 cut(s)	20
AccBSI	CCGCTC	1 cut(s)	18
AciI	CCGC	9 cut(s)	18, 24, 36, 291, 338, 718, 1031, 1039, 1042
AclI	AACGTT	1 cut(s)	198
AclWI	GGATC	1 cut(s)	1213
AcoI	YGGCCR	2 cut(s)	639, 1014
AcsI	RAATTY	2 cut(s)	757, 1021
AcyI	GRCGYC	3 cut(s)	21, 1115, 1437
AfaI	GTAC	7 cut(s)	188, 529, 600, 782, 899, 1089, 1542
AfiI	CCNNNNNNNGG	5 cut(s)	685, 686, 782, 1125, 1432
AflIII	ACRYGT	1 cut(s)	1368
AgeI	ACCGGT	1 cut(s)	968
AgsI	TTSAA	7 cut(s)	156, 163, 871, 892, 1147, 1224, 1465
AhdI	GACNNNNNGTC	1 cut(s)	55
AjnI	CCWGG	3 cut(s)	423, 776, 1387
AloI	GAACNNNNNNTCC	2 cut(s)	1499, 1531
Alw21I	GWGCWC	2 cut(s)	694, 1287
Alw26I	GTCTC	4 cut(s)	33, 978, 1378, 1397
AlwI	GGATC	1 cut(s)	1213
AlwNI	CAGNNNCTG	1 cut(s)	655
Ama87I	CYCGRG	1 cut(s)	133
AoxI	GGCC	5 cut(s)	312, 501, 639, 1014, 1413
ApeKI	GCWGC	5 cut(s)	53, 429, 652, 1213, 1458
ApoI	RAATTY	2 cut(s)	757, 1021
ArsI	GACNNNNNNTTYG	4 cut(s)	596, 602, 628, 634
AseI	ATTAAT	1 cut(s)	1167
AsiGI	ACCGGT	1 cut(s)	968
Asp700I	GAANNNNTTC	1 cut(s)	943
AspLEI	GCGC	4 cut(s)	23, 551, 647, 717
AspS9I	GGNCC	4 cut(s)	63, 502, 773, 1413
AsuC2I	CCSGG	2 cut(s)	662, 1203
AsuHPI	GGTGA	4 cut(s)	191, 755, 845, 958
AsuII	TTCGAA	2 cut(s)	621, 947
AvaI	CYCGRG	1 cut(s)	133
AvaII	GGWCC	2 cut(s)	63, 773
BanI	GGYRCC	1 cut(s)	20
BanII	GRGCYC	3 cut(s)	114, 452, 1287
BbsI	GAAGAC	1 cut(s)	51
Bbv12I	GWGCWC	2 cut(s)	694, 1287
BbvI	GCAGC	5 cut(s)	65, 416, 639, 1200, 1445
BccI	CCATC	8 cut(s)	25, 484, 626, 731, 832, 1021, 1099, 1261
BceAI	ACGGC	3 cut(s)	516, 622, 1254
BciT130I	CCWGG	3 cut(s)	425, 778, 1389
BclI	TGATCA	1 cut(s)	1452
BcnI	CCSGG	2 cut(s)	662, 1203
BcoDI	GTCTC	4 cut(s)	33, 978, 1378, 1397
BfaI	CTAG	1 cut(s)	1124
BfmI	CTRYAG	1 cut(s)	729
BfoI	RGCGCY	4 cut(s)	24, 552, 648, 718
BlpI	GCTNAGC	5 cut(s)	648, 666, 693, 1304, 1494
BmcAI	AGTACT	1 cut(s)	1542
Bme1390I	CCNGG	5 cut(s)	425, 662, 778, 1203, 1389
Bme18I	GGWCC	2 cut(s)	63, 773
BmeRI	GACNNNNNGTC	1 cut(s)	55
BmeT110I	CYCGRG	1 cut(s)	133
BmgT120I	GGNCC	4 cut(s)	63, 502, 773, 1413
BmiI	GGNNCC	4 cut(s)	22, 451, 774, 775
BmrFI	CCNGG	5 cut(s)	425, 662, 778, 1203, 1389
BmsI	GCATC	1 cut(s)	1120
BpiI	GAAGAC	1 cut(s)	51
BpmI	CTGGAG	2 cut(s)	815, 1208
Bpu10I	CCTNAGC	1 cut(s)	1076
Bpu1102I	GCTNAGC	5 cut(s)	648, 666, 693, 1304, 1494
Bpu14I	TTCGAA	2 cut(s)	621, 947
BpuEI	CTTGAG	1 cut(s)	201
BpuMI	CCSGG	2 cut(s)	662, 1203
BsaHI	GRCGYC	3 cut(s)	21, 1115, 1437
BsaI	GGTCTC	3 cut(s)	978, 1378, 1397
BsaJI	CCNNGG	4 cut(s)	424, 684, 776, 1416
BsaWI	WCCGGW	4 cut(s)	577, 863, 968, 987
BsaXI	ACNNNNNCTCC	6 cut(s)	250, 280, 1234, 1264, 1375, 1405
Bsc4I	CCNNNNNNNGG	5 cut(s)	685, 686, 782, 1125, 1432
Bse118I	RCCGGY	4 cut(s)	310, 641, 968, 1012
Bse1I	ACTGG	4 cut(s)	188, 564, 798, 1513
Bse3DI	GCAATG	1 cut(s)	1505
BseBI	CCWGG	3 cut(s)	425, 778, 1389
BseDI	CCNNGG	4 cut(s)	424, 684, 776, 1416
BseGI	GGATG	6 cut(s)	36, 862, 889, 1135, 1234, 1253
BseLI	CCNNNNNNNGG	5 cut(s)	685, 686, 782, 1125, 1432
BseMI	GCAATG	1 cut(s)	1505
BseMII	CTCAG	6 cut(s)	217, 553, 662, 707, 1331, 1485
BseNI	ACTGG	4 cut(s)	188, 564, 798, 1513
BseRI	GAGGAG	8 cut(s)	258, 277, 364, 371, 842, 857, 932, 996
BseX3I	CGGCCG	1 cut(s)	639
BseXI	GCAGC	5 cut(s)	65, 416, 639, 1200, 1445
BseYI	CCCAGC	2 cut(s)	211, 437
Bsh1285I	CGRYCG	1 cut(s)	642
BshFI	GGCC	5 cut(s)	314, 503, 641, 1016, 1415
BshNI	GGYRCC	1 cut(s)	20
BshTI	ACCGGT	1 cut(s)	968
BsiEI	CGRYCG	1 cut(s)	642
BsiHKAI	GWGCWC	2 cut(s)	694, 1287
BsiHKCI	CYCGRG	1 cut(s)	133
BsiSI	CCGG	9 cut(s)	311, 578, 642, 661, 864, 969, 988, 1013, 1203
BslFI	GGGAC	1 cut(s)	759
BslI	CCNNNNNNNGG	5 cut(s)	685, 686, 782, 1125, 1432
BsmAI	GTCTC	4 cut(s)	33, 978, 1378, 1397
BsmBI	CGTCTC	1 cut(s)	33
BsmFI	GGGAC	1 cut(s)	759
BsmI	GAATGC	2 cut(s)	928, 955
BsnI	GGCC	5 cut(s)	314, 503, 641, 1016, 1415
Bso31I	GGTCTC	3 cut(s)	978, 1378, 1397
BsoBI	CYCGRG	1 cut(s)	133
Bsp119I	TTCGAA	2 cut(s)	621, 947
Bsp1286I	GDGCHC	4 cut(s)	114, 452, 694, 1287
Bsp143I	GATC	2 cut(s)	1205, 1452
Bsp1720I	GCTNAGC	5 cut(s)	648, 666, 693, 1304, 1494
Bsp19I	CCATGG	1 cut(s)	684
BspACI	CCGC	9 cut(s)	18, 24, 36, 291, 338, 718, 1031, 1039, 1042
BspANI	GGCC	5 cut(s)	314, 503, 641, 1016, 1415
BspCNI	CTCAG	6 cut(s)	216, 552, 661, 706, 1330, 1486
BspHI	TCATGA	1 cut(s)	1564
BspLI	GGNNCC	4 cut(s)	22, 451, 774, 775
BspPI	GGATC	1 cut(s)	1213
BspQI	GCTCTTC	1 cut(s)	1275
BspT104I	TTCGAA	2 cut(s)	621, 947
BspT107I	GGYRCC	1 cut(s)	20
BspTNI	GGTCTC	3 cut(s)	978, 1378, 1397
BsrBI	CCGCTC	1 cut(s)	18
BsrDI	GCAATG	1 cut(s)	1505
BsrFI	RCCGGY	4 cut(s)	310, 641, 968, 1012
BsrI	ACTGG	4 cut(s)	188, 564, 798, 1513
BssAI	RCCGGY	4 cut(s)	310, 641, 968, 1012
BssECI	CCNNGG	4 cut(s)	424, 684, 776, 1416
BssMI	GATC	2 cut(s)	1205, 1452
BssNI	GRCGYC	3 cut(s)	21, 1115, 1437
BssT1I	CCWWGG	2 cut(s)	684, 1416
Bst2UI	CCWGG	3 cut(s)	425, 778, 1389
Bst4CI	ACNGT	3 cut(s)	902, 964, 1357
Bst6I	CTCTTC	3 cut(s)	241, 812, 1275
BstACI	GRCGYC	3 cut(s)	21, 1115, 1437
BstAPI	GCANNNNNTGC	1 cut(s)	352
BstBI	TTCGAA	2 cut(s)	621, 947
BstC8I	GCNNGC	5 cut(s)	312, 643, 1014, 1218, 1446
BstDEI	CTNAG	9 cut(s)	203, 539, 648, 666, 693, 1076, 1304, 1317, 1494
BstDSI	CCRYGG	1 cut(s)	684
BstF5I	GGATG	6 cut(s)	36, 862, 889, 1135, 1234, 1253
BstH2I	RGCGCY	4 cut(s)	24, 552, 648, 718
BstHHI	GCGC	4 cut(s)	23, 551, 647, 717
BstKTI	GATC	2 cut(s)	1208, 1455
BstMAI	GTCTC	4 cut(s)	33, 978, 1378, 1397
BstMBI	GATC	2 cut(s)	1205, 1452
BstMCI	CGRYCG	1 cut(s)	642
BstMWI	GCNNNNNNNGC	7 cut(s)	343, 352, 615, 624, 633, 642, 1072
BstNI	CCWGG	3 cut(s)	425, 778, 1389
BstNSI	RCATGY	2 cut(s)	1075, 1372
BstSCI	CCNGG	5 cut(s)	423, 660, 776, 1201, 1387
BstSFI	CTRYAG	1 cut(s)	729
BstV1I	GCAGC	5 cut(s)	65, 416, 639, 1200, 1445
BstV2I	GAAGAC	1 cut(s)	51
BstX2I	RGATCY	1 cut(s)	1205
BstYI	RGATCY	1 cut(s)	1205
BstZI	CGGCCG	1 cut(s)	639
BsuRI	GGCC	5 cut(s)	314, 503, 641, 1016, 1415
BtgI	CCRYGG	1 cut(s)	684
BtgZI	GCGATG	1 cut(s)	750
BtsCI	GGATG	6 cut(s)	36, 862, 889, 1135, 1234, 1253
BtsIMutI	CAGTG	1 cut(s)	960
Cac8I	GCNNGC	5 cut(s)	312, 643, 1014, 1218, 1446
CaiI	CAGNNNCTG	1 cut(s)	655
CciI	TCATGA	1 cut(s)	1564
CfoI	GCGC	4 cut(s)	23, 551, 647, 717
Cfr10I	RCCGGY	4 cut(s)	310, 641, 968, 1012
Cfr13I	GGNCC	4 cut(s)	63, 502, 773, 1413
CseI	GACGC	4 cut(s)	45, 59, 443, 1104
CsiI	ACCWGGT	1 cut(s)	1387
Csp6I	GTAC	7 cut(s)	187, 528, 599, 781, 898, 1088, 1541
CspAI	ACCGGT	1 cut(s)	968
CviAII	CATG	8 cut(s)	190, 227, 515, 685, 1072, 1369, 1408, 1565
CviQI	GTAC	7 cut(s)	187, 528, 599, 781, 898, 1088, 1541
DdeI	CTNAG	9 cut(s)	203, 539, 648, 666, 693, 1076, 1304, 1317, 1494
DinI	GGCGCC	1 cut(s)	22
DpnI	GATC	2 cut(s)	1207, 1454
DpnII	GATC	2 cut(s)	1205, 1452
DriI	GACNNNNNGTC	1 cut(s)	55
EaeI	YGGCCR	2 cut(s)	639, 1014
EagI	CGGCCG	1 cut(s)	639
Eam1104I	CTCTTC	3 cut(s)	241, 812, 1275
Eam1105I	GACNNNNNGTC	1 cut(s)	55
EarI	CTCTTC	3 cut(s)	241, 812, 1275
EciI	GGCGGA	4 cut(s)	51, 280, 1046, 1057
Ecl136II	GAGCTC	1 cut(s)	1285
EclXI	CGGCCG	1 cut(s)	639
Eco130I	CCWWGG	2 cut(s)	684, 1416
Eco24I	GRGCYC	3 cut(s)	114, 452, 1287
Eco31I	GGTCTC	3 cut(s)	978, 1378, 1397
Eco47I	GGWCC	2 cut(s)	63, 773
Eco52I	CGGCCG	1 cut(s)	639
Eco53kI	GAGCTC	1 cut(s)	1285
Eco88I	CYCGRG	1 cut(s)	133
EcoICRI	GAGCTC	1 cut(s)	1285
EcoO109I	RGGNCCY	2 cut(s)	773, 1413
EcoRII	CCWGG	3 cut(s)	423, 776, 1387
EcoT14I	CCWWGG	2 cut(s)	684, 1416
EcoT38I	GRGCYC	3 cut(s)	114, 452, 1287
EgeI	GGCGCC	1 cut(s)	22
EheI	GGCGCC	1 cut(s)	22
ErhI	CCWWGG	2 cut(s)	684, 1416
Esp3I	CGTCTC	1 cut(s)	33
FaeI	CATG	8 cut(s)	193, 230, 518, 688, 1075, 1372, 1411, 1568
FalI	AAGNNNNNCTT	2 cut(s)	1527, 1559
FaqI	GGGAC	1 cut(s)	759
FatI	CATG	8 cut(s)	189, 226, 514, 684, 1071, 1368, 1407, 1564
FbaI	TGATCA	1 cut(s)	1452
FokI	GGATG	6 cut(s)	43, 869, 896, 1142, 1221, 1240
FriOI	GRGCYC	3 cut(s)	114, 452, 1287
FspBI	CTAG	1 cut(s)	1124
GlaI	GCGC	4 cut(s)	22, 550, 646, 716
GsaI	CCCAGC	2 cut(s)	215, 441
GsuI	CTGGAG	2 cut(s)	815, 1208
HaeII	RGCGCY	4 cut(s)	24, 552, 648, 718
HaeIII	GGCC	5 cut(s)	314, 503, 641, 1016, 1415
HapII	CCGG	9 cut(s)	311, 578, 642, 661, 864, 969, 988, 1013, 1203
HgaI	GACGC	4 cut(s)	45, 59, 443, 1104
HhaI	GCGC	4 cut(s)	23, 551, 647, 717
Hin1I	GRCGYC	3 cut(s)	21, 1115, 1437
Hin1II	CATG	8 cut(s)	193, 230, 518, 688, 1075, 1372, 1411, 1568
Hin6I	GCGC	4 cut(s)	21, 549, 645, 715
HinP1I	GCGC	4 cut(s)	21, 549, 645, 715
HincII	GTYRAC	1 cut(s)	1242
HindII	GTYRAC	1 cut(s)	1242
HindIII	AAGCTT	1 cut(s)	246
HinfI	GANTC	6 cut(s)	131, 284, 850, 878, 1190, 1550
HpaII	CCGG	9 cut(s)	311, 578, 642, 661, 864, 969, 988, 1013, 1203
HphI	GGTGA	4 cut(s)	191, 755, 845, 958
Hpy166II	GTNNAC	1 cut(s)	1242
Hpy188I	TCNGA	6 cut(s)	9, 253, 489, 883, 1383, 1490
Hpy188III	TCNNGA	6 cut(s)	218, 323, 533, 709, 854, 1565
Hpy8I	GTNNAC	1 cut(s)	1242
Hpy99I	CGWCG	5 cut(s)	53, 310, 392, 437, 608
HpyCH4III	ACNGT	3 cut(s)	902, 964, 1357
HpyCH4IV	ACGT	3 cut(s)	198, 387, 1437
HpyCH4V	TGCA	3 cut(s)	274, 1133, 1503
HpyF10VI	GCNNNNNNNGC	7 cut(s)	343, 352, 615, 624, 633, 642, 1072
HpyF3I	CTNAG	9 cut(s)	203, 539, 648, 666, 693, 1076, 1304, 1317, 1494
HpySE526I	ACGT	3 cut(s)	198, 387, 1437
Hsp92I	GRCGYC	3 cut(s)	21, 1115, 1437
Hsp92II	CATG	8 cut(s)	193, 230, 518, 688, 1075, 1372, 1411, 1568
HspAI	GCGC	4 cut(s)	21, 549, 645, 715
KasI	GGCGCC	1 cut(s)	20
KflI	GGGWCCC	1 cut(s)	773
KroI	GCCGGC	3 cut(s)	310, 641, 1012
KroNI	GCCGGC	3 cut(s)	312, 643, 1014
Ksp22I	TGATCA	1 cut(s)	1452
Kzo9I	GATC	2 cut(s)	1205, 1452
LguI	GCTCTTC	1 cut(s)	1275
LmnI	GCTCC	7 cut(s)	143, 271, 401, 455, 675, 1201, 1301
Lsp1109I	GCAGC	5 cut(s)	65, 416, 639, 1200, 1445
LweI	GCATC	1 cut(s)	1120
MabI	ACCWGGT	1 cut(s)	1387
MaeI	CTAG	1 cut(s)	1124
MaeII	ACGT	3 cut(s)	198, 387, 1437
MaeIII	GTNAC	3 cut(s)	179, 580, 964
MalI	GATC	2 cut(s)	1207, 1454
MbiI	CCGCTC	1 cut(s)	18
MboI	GATC	2 cut(s)	1205, 1452
MboII	GAAGA	9 cut(s)	56, 129, 228, 820, 829, 940, 1012, 1060, 1292
MfeI	CAATTG	1 cut(s)	1134
MflI	RGATCY	1 cut(s)	1205
MhlI	GDGCHC	4 cut(s)	114, 452, 694, 1287
MluCI	AATT	8 cut(s)	475, 508, 749, 757, 871, 943, 1021, 1134
Mly113I	GGCGCC	1 cut(s)	21
MlyI	GAGTC	5 cut(s)	125, 278, 859, 872, 1199
MmeI	TCCRAC	1 cut(s)	423
MroNI	GCCGGC	3 cut(s)	310, 641, 1012
MroXI	GAANNNNTTC	1 cut(s)	943
MseI	TTAA	3 cut(s)	1167, 1275, 1359
MspA1I	CMGCKG	3 cut(s)	652, 1458, 1556
MspI	CCGG	9 cut(s)	311, 578, 642, 661, 864, 969, 988, 1013, 1203
MspR9I	CCNGG	5 cut(s)	425, 662, 778, 1203, 1389
MunI	CAATTG	1 cut(s)	1134
Mva1269I	GAATGC	2 cut(s)	928, 955
MvaI	CCWGG	3 cut(s)	425, 778, 1389
MwoI	GCNNNNNNNGC	7 cut(s)	343, 352, 615, 624, 633, 642, 1072
NaeI	GCCGGC	3 cut(s)	312, 643, 1014
NarI	GGCGCC	1 cut(s)	21
NciI	CCSGG	2 cut(s)	662, 1203
NcoI	CCATGG	1 cut(s)	684
NdeII	GATC	2 cut(s)	1205, 1452
NgoMIV	GCCGGC	3 cut(s)	310, 641, 1012
NlaIII	CATG	8 cut(s)	193, 230, 518, 688, 1075, 1372, 1411, 1568
NlaIV	GGNNCC	4 cut(s)	22, 451, 774, 775
NmeAIII	GCCGAG	2 cut(s)	577, 617
NmuCI	GTSAC	2 cut(s)	179, 964
NspI	RCATGY	2 cut(s)	1075, 1372
NspV	TTCGAA	2 cut(s)	621, 947
PaeR7I	CTCGAG	1 cut(s)	133
PagI	TCATGA	1 cut(s)	1564
PciI	ACATGT	1 cut(s)	1368
PciSI	GCTCTTC	1 cut(s)	1275
PcsI	WCGNNNNNNNCGW	1 cut(s)	627
PctI	GAATGC	2 cut(s)	928, 955
PdiI	GCCGGC	3 cut(s)	312, 643, 1014
PdmI	GAANNNNTTC	1 cut(s)	943
PfeI	GAWTC	1 cut(s)	1550
PflFI	GACNNNGTC	1 cut(s)	1389
PinAI	ACCGGT	1 cut(s)	968
PleI	GAGTC	5 cut(s)	125, 278, 858, 872, 1198
PluTI	GGCGCC	1 cut(s)	24
PpsI	GAGTC	5 cut(s)	125, 278, 858, 872, 1198
PpuMI	RGGWCCY	1 cut(s)	773
PscI	ACATGT	1 cut(s)	1368
PshBI	ATTAAT	1 cut(s)	1167
Psp124BI	GAGCTC	1 cut(s)	1287
Psp1406I	AACGTT	1 cut(s)	198
Psp5II	RGGWCCY	1 cut(s)	773
Psp6I	CCWGG	3 cut(s)	423, 776, 1387
PspFI	CCCAGC	2 cut(s)	211, 437
PspGI	CCWGG	3 cut(s)	423, 776, 1387
PspN4I	GGNNCC	4 cut(s)	22, 451, 774, 775
PspPI	GGNCC	4 cut(s)	63, 502, 773, 1413
PspPPI	RGGWCCY	1 cut(s)	773
PspXI	VCTCGAGB	1 cut(s)	133
PstNI	CAGNNNCTG	1 cut(s)	655
PsuI	RGATCY	1 cut(s)	1205
PsyI	GACNNNGTC	1 cut(s)	1389
PvuII	CAGCTG	3 cut(s)	652, 1458, 1556
RsaI	GTAC	7 cut(s)	188, 529, 600, 782, 899, 1089, 1542
RsaNI	GTAC	7 cut(s)	187, 528, 599, 781, 898, 1088, 1541
SacI	GAGCTC	1 cut(s)	1287
SapI	GCTCTTC	1 cut(s)	1275
SaqAI	TTAA	3 cut(s)	1167, 1275, 1359
Sau3AI	GATC	2 cut(s)	1205, 1452
Sau96I	GGNCC	4 cut(s)	63, 502, 773, 1413
ScaI	AGTACT	1 cut(s)	1542
SchI	GAGTC	5 cut(s)	125, 278, 859, 872, 1199
ScrFI	CCNGG	5 cut(s)	425, 662, 778, 1203, 1389
SduI	GDGCHC	4 cut(s)	114, 452, 694, 1287
SexAI	ACCWGGT	1 cut(s)	1387
SfaNI	GCATC	1 cut(s)	1120
SfcI	CTRYAG	1 cut(s)	729
SfoI	GGCGCC	1 cut(s)	22
Sfr274I	CTCGAG	1 cut(s)	133
SfuI	TTCGAA	2 cut(s)	621, 947
SgrAI	CRCCGGYG	1 cut(s)	968
SinI	GGWCC	2 cut(s)	63, 773
SlaI	CTCGAG	1 cut(s)	133
SmlI	CTYRAG	2 cut(s)	133, 216
SmoI	CTYRAG	2 cut(s)	133, 216
Sse9I	AATT	8 cut(s)	475, 508, 749, 757, 871, 943, 1021, 1134
SsiI	CCGC	9 cut(s)	18, 24, 36, 291, 338, 718, 1031, 1039, 1042
SspDI	GGCGCC	1 cut(s)	20
SspMI	CTAG	1 cut(s)	1124
SstI	GAGCTC	1 cut(s)	1287
StyD4I	CCNGG	5 cut(s)	423, 660, 776, 1201, 1387
StyI	CCWWGG	2 cut(s)	684, 1416
TaaI	ACNGT	3 cut(s)	902, 964, 1357
TaiI	ACGT	3 cut(s)	201, 390, 1440
TasI	AATT	8 cut(s)	475, 508, 749, 757, 871, 943, 1021, 1134
TatI	WGTACW	3 cut(s)	598, 897, 1540
TauI	GCSGC	5 cut(s)	21, 26, 340, 720, 1042
TfiI	GAWTC	1 cut(s)	1550
Tru1I	TTAA	3 cut(s)	1167, 1275, 1359
Tru9I	TTAA	3 cut(s)	1167, 1275, 1359
TscAI	CASTG	1 cut(s)	967
TseFI	GTSAC	2 cut(s)	179, 964
TseI	GCWGC	5 cut(s)	53, 429, 652, 1213, 1458
Tsp45I	GTSAC	2 cut(s)	179, 964
TspDTI	ATGAA	3 cut(s)	206, 1223, 1553
TspGWI	ACGGA	4 cut(s)	294, 599, 798, 1227
TspRI	CASTG	1 cut(s)	967
Tth111I	GACNNNGTC	1 cut(s)	1389
VpaK11BI	GGWCC	2 cut(s)	63, 773
VspI	ATTAAT	1 cut(s)	1167
XapI	RAATTY	2 cut(s)	757, 1021
XceI	RCATGY	2 cut(s)	1075, 1372
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1024, 1414
XhoI	CTCGAG	1 cut(s)	133
XmnI	GAANNNNTTC	1 cut(s)	943
XspI	CTAG	1 cut(s)	1124
ZraI	GACGTC	1 cut(s)	1438
ZrmI	AGTACT	1 cut(s)	1542

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.