Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0002376

Physical Location & Seq

Genomic Coordinates Reverse (-)

34990510 .. 34998136

Gene Structure 7627 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1217900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3681 bp

ATGGCGGCGCCGTTAACGGATGGAGGCGGAGGACTGGTGGTTCTGTCGAATTCCGTCAACAAGGTGGAATCGTCATCGGTCAATGGCTGCTTGGCGAGCACGGAGGAGCCGACAAGGAGGCCGATTCTGATTTTCCTGTTCTTTCACAAGGCAATTCGGAAGGAGCTCGATGCGCTCCACCGACTGGCCATGGCCTTCGCCACCGGAAAGAGAACCGATATACGGCCGCTGCTCGAGCGCTACCATTTTTTGAGATCGATTTACAAGCACCACTCCAATGCCGAAGACGAGGTAATCTTTCCAGCTCTTGATATACGCGTGAAGAATGTAGCACAAACATACTCGCTTGAGCATAAGGGCGAAACAAATCTTTTTGATCATCTATTTGAGCTGCTAAGTTCAAATGCGAAAAATGATGAAAATTTCCCAAGAGAGCTAGCGTCTTGTACAGGAGCTCTACAAACATCAGTTAGCCAACACCTGGCAAAGGAGGAGGAACAGGTCCTTCCCTTACTCATTGAGAAATTTTCAGTTGAAGAGCAGGCAGCTCTGGTCTGGCAGTTTCTGTGCAGCATTCCTGTGAATATGATGGCCGAGTTCCTTCCATGGCTTTCATCATCTGTTTCACCTGATGAACATCAGGATTTGCAAAAATGCTTAAGGAAGATTGTTCCAGAGGAAAAGCTTCTTCAACAAGTCATTTTCACCTGGATGGGAGGTAGAAACAGTGTTGACATGTTCGGAAGTTGTCTAGATGCCCCTCAGTTCCAATGTTGTGTGGATTCTGGTGCTAGCACATCAAGTCAACATATGGAGAAGGAAAATTCTGCATGCGAGTGCAGGACTGGGAAAAGGAAGTACTTAGAATCAAGTACTGATGTTTCTGATAACATTGCAGGGCACCCAATAAATGAAATACTTCTTTGGCATAATGCCATTAAAAGGGAGTTAAATGAGATAGCTGAGGAGGCTAGGAAGATACAATGTTATGGAGATTTTACTAATATATCAGCTTTCAATGAGAGGTTGCAATTTATCGCTGAAGTTTGCATCTTTCACAGTATTGCTGAGGACAAGGTCATATTTCCAGCAGTTGATGGAAAAATTTCCTTTTTCCAAGAGCATGCTGAAGAAGAAAGTCAATTCAACGAATTCAGGTGTTTGATTGAAAATATTCAAAGTGCAGGAGCCATCTCTACTTCCGCTGATTTTTATGCAAAGTTATGTTCTCATGCTGATCAAATAATGGAAACTATCCAGAGGCACTTTAGCAATGAAGAAGTTCAGGTTCTTCCACTTGCTCGAGAGCACTTCAGCTTCAAAAGGCAGCGTGAACTTTTGTATCAATCTTTGTGTATGATGCCCTTAAGACTGATTGAGCGTGTTTTACCTTGGCTGGTTGGAACGTTGACTGAAGATGAAACAAAGAATTTTCTCAAAAACATGCAGTTAGCAGTGCCTGATTCTGCTTTAGTAACACTCTTTTCGGGATGGGCATGCAAGGCTCGTAGTCAGGGTTCATGCTTGTCGTCAAGTGCAATTGGTTGCTGTCCTGTTAAAAGCTTCACTGATATTGAAGAAGGTTTTGTTCGTTCAGCATGTGCGTGTTCTTCCGCATCGTCTGCTAGAGACAGCCTGACACCAGCTCAAGCAAATAATGTGAAGAGGCCAGCCAAACGAAATGTTTCTATGTCATGCAAACACAATGGTAGTGACCAGTCCTGCCGTGTCCCAGGTTTAGGAGTGAACAGCAATAATCTTGGGCCGAGTTCTCTTTTTGGGGCCAAGTCTTTGCGATCCTTATCTTTCAGCTCTGGTGCCCCATCACTGAATTCCAGTCTTTTTGTCTGGGAAATGGATCATTCTTCTTCTGATATTGGCTGTGGGGAACGCCCTATTGATACTATTTTTAAATTTCATAAAGCCATACGCAAAGATTTGGAGTATTTAGATAGTGAATCTGGAAAGCTTAGTTATTGTGATGAAACCACTCTTCGGCAGTTCATAGGAAGGTTTCGTTTACTGTGGGGATTATACAGAGCGCATAGTAATGCGGAGGATGATATAGTGTTTCCTGCACTGGAATCCAAAGAGGCGCTTCATAATGTGAGTCACTCATACACACTGGACCATAAACAGGAGGAGAAATTATTTGAAGATATCTCATGTGTTCTGTCGGAGCTTTCTCACCTTCATAAAAGCTTGCAAAAGGATCACATGGACGAGGATTCAGCTGGAAGTAGCATTGGCTTTTCTGCTGCTAATGGTAACTACTCTAAAAAGTATGACAAGCTAGCTACGAAGCTTCAAGGAATGTGCAAATCAATCAAGGTTACACTAGATCAGCATATTTTCAGGGAAGAACTGGAGCTGTGGCCATTATTCGGCAGACACTTCACTGTGGAGGAGCAAGACAAAATAGTTGGCCGCATTATTGGGACCACGGGTGCTGAGGTGCTCCAATCAATGTTACCATGGGTGACTTCTGCACTTACTCAGGATGAACAGAACAAAATGATGGATACGTGGAAGCAAGCAACTAAAAATACAATGTTCAATGAGTGGCTTAATGAATGCTGGAAAGGAATTTCAGAATTAACTTCACAGACTGAAACATCGGAAAGTAGCACTCCTCAAAAAGGGGTTGAATTTCAAGAAAGCTTGGATCAGACTGATCAGATGTTCAAACCTGGTTGGAAGGATATTTTCCGTATGAATCAAAATGAACTTGAGTCAGAGATCAGAAAGGTTTATCGTGATGCGACTCTTGATCCAAGGAGAAAGGCATATCTTGTGCAGAATCTTATGACTAGCCGCTGGATAGCTACTCAGCAGAAGTTACCTCAAGAAATAGCTGGAGAATCATCTAATGGTGAAGACATGGTTGGACACTCACCAATGTATCGAGATGCTGAGAAAAAAGAGTTTGGGTGTGAACACTATAAAAGAAACTGTAAACTCCGAGCTGCTTGCTGTGGCAAGTTATTTACGTGTAGATTTTGCCATGACAATGTGAGCGATCATTCAATGGATAGGAAAGCAACATCTGAAATGATGTGCATGCGCTGCCTGACCGTTCAGCCTGTTGGGCCAATATGCACTACACCTTCATGCAATGAACTCTCCATGGCAAAGTACTACTGCAATATATGTAAATTTTTTGACGATGAAAGGACTGTGTATCATTGCCCATTTTGCAATTTATGCCGACTTGGAAAGGGTCTTGGCATTGACTTCTTTCATTGCATGACATGCAATTGTTGCCTGGGGATAAAGTTAGTGAACCACAAGTGCCTGGAGAAGAGTTTAGAAACAAATTGCCCCATCTGCTGTGATTTCTTATTCACATCAAGTGCAACGGTTCGAGCTCTGCCTTGTGGCCACTACATGCATTCTGCTTGCTTTCAGGCATACACTTCCAGTCACTATACTTGTCCAATTTGCAGCAAATCTTTGGGAGATATGGCAGTTTACTTTGGCATGCTTGACGCATTATTGGCTGCCGAGCAGCTTCCAGAGGAATACAGGAACCGTTGTCAGGACATTCTATGTAACGACTGTGATCGAAAGGGTACCTCACGGTTTCACTGGCTATATCACAAGTGCGGGAACTGCGGATCTTACAACACCCGGGTGATCAAGGGGGAGACATCATATACCGACTGCTCAACACCGAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hemerythrin zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

1227

Amino Acids

138.46

Weight (kDa)

5.94

Isoelectric Point (pI)

52.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hemerythrin	PF01814	47 - 172	1.1e-10	Hemerythrin HHE cation binding domain
Hemerythrin	PF01814	632 - 793	4.8e-06	Hemerythrin HHE cation binding domain
zf-CHY	PF05495	978 - 1046	1.4e-19	CHY zinc finger
zf-RING_2	PF13639	1106 - 1148	1.6e-08	Ring finger domain
zinc_ribbon_6	PF14599	1154 - 1211	9.9e-24	Zinc-ribbon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18290
fragaria_vesca	FvH4_4g26990 FvH4_4g26990 FvH4_4g26990 FvH4_4g26990 FvH4_5g11860 FvH4_5g11860 FvH4_5g11860
malus_domestica	MD06G1217900.v1.1 MD14G1228100.v1.1 MD16G1039900.v1.1
prunus_persica	Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1
pyrus_communis	pycom06g19410 pycom14g18950 pycom16g03460
rosa_chinensis	RchiOBHm_Chr4g0434791 RchiOBHm_Chr7g0185731
rosa_laevigata	RLG00000004888 RLG00000006649
rosa_multiflora	Rmu_sc0000179.1_g000001 Rmu_sc0002952.1_g000002
rosa_roxburghii	Rroxscaffold_3G00268670 Rroxscaffold_5G00376000
rosa_rugosa	Rorug04G0278300 Rorug04G0278400 Rorug06G0470900
rosa_samantha	Rh4AG334900 Rh4CG357800 Rh4DG337300 Rh7AG071600 Rh7BG076200 Rh7DG076200
rosa_wichuraiana	Rw4G029200 Rw7G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	3572
AccB1I	GGYRCC	4 cut(s)	7, 900, 1817, 3572
AccB7I	CCANNNNNTGG	2 cut(s)	184, 481
AccII	CGCG	1 cut(s)	318
AclI	AACGTT	1 cut(s)	1406
AclWI	GGATC	6 cut(s)	1791, 1866, 2220, 2673, 2765, 3625
AcoI	YGGCCR	6 cut(s)	186, 224, 591, 2375, 2425, 3379
AcuI	CTGAAG	4 cut(s)	1062, 1149, 1297, 1434
AcyI	GRCGYC	1 cut(s)	8
AfaI	GTAC	5 cut(s)	448, 860, 874, 3137, 3574
AfeI	AGCGCT	1 cut(s)	239
AflII	CTTAAG	2 cut(s)	658, 1366
AflIII	ACRYGT	3 cut(s)	316, 735, 2990
AjnI	CCWGG	6 cut(s)	480, 707, 1732, 2689, 3264, 3294
AjuI	GAANNNNNNNTTGG	2 cut(s)	2868, 2900
AleI	CACNNNNGTG	1 cut(s)	3632
AloI	GAACNNNNNNTCC	2 cut(s)	24, 56
Alw21I	GWGCWC	6 cut(s)	101, 168, 457, 1311, 2460, 3370
Alw26I	GTCTC	2 cut(s)	1623, 3641
AlwI	GGATC	6 cut(s)	1791, 1866, 2220, 2673, 2765, 3625
AlwNI	CAGNNNCTG	2 cut(s)	565, 1460
Ama87I	CYCGRG	3 cut(s)	233, 1302, 3630
Aor51HI	AGCGCT	1 cut(s)	239
Asp700I	GAANNNNTTC	6 cut(s)	684, 918, 1105, 1173, 1281, 1684
Asp718I	GGTACC	1 cut(s)	3572
AspLEI	GCGC	6 cut(s)	10, 175, 240, 2044, 2098, 3066
AspS9I	GGNCC	6 cut(s)	502, 1763, 1782, 2128, 2439, 3089
AsuC2I	CCSGG	2 cut(s)	3631, 3632
AsuHPI	GGTGA	7 cut(s)	618, 697, 2180, 2491, 2885, 2886, 3646
AsuNHI	GCTAGC	3 cut(s)	436, 791, 2293
AvaI	CYCGRG	3 cut(s)	233, 1302, 3630
AvaII	GGWCC	3 cut(s)	502, 2128, 2439
BaeGI	GKGCMC	2 cut(s)	903, 1822
BaeI	ACNNNNGTAYC	4 cut(s)	1325, 1358, 2885, 2918
BalI	TGGCCA	3 cut(s)	188, 2377, 3381
BanI	GGYRCC	4 cut(s)	7, 900, 1817, 3572
BanII	GRGCYC	3 cut(s)	168, 457, 3370
BbsI	GAAGAC	2 cut(s)	291, 2883
Bbv12I	GWGCWC	6 cut(s)	101, 168, 457, 1311, 2460, 3370
BbvCI	CCTCAGC	3 cut(s)	963, 1068, 2451
BccI	CCATC	9 cut(s)	14, 583, 706, 1091, 1199, 1485, 1831, 2512, 3332
BceAI	ACGGC	2 cut(s)	239, 1710
BcgI	CGANNNNNNTGC	2 cut(s)	2952, 2986
BciT130I	CCWGG	6 cut(s)	482, 709, 1734, 2691, 3266, 3296
BciVI	GTATCC	1 cut(s)	2515
BclI	TGATCA	4 cut(s)	376, 1237, 2674, 3636
BcnI	CCSGG	2 cut(s)	3631, 3632
BcoDI	GTCTC	2 cut(s)	1623, 3641
BfaI	CTAG	8 cut(s)	437, 752, 792, 972, 1626, 2294, 2339, 2811
BfoI	RGCGCY	3 cut(s)	11, 241, 2099
BfrI	CTTAAG	2 cut(s)	658, 1366
BfuI	GTATCC	1 cut(s)	2515
BglI	GCCNNNNNGGC	1 cut(s)	3088
BmcAI	AGTACT	3 cut(s)	860, 874, 3137
Bme1390I	CCNGG	8 cut(s)	482, 709, 1734, 2691, 3266, 3296, 3631, 3632
Bme18I	GGWCC	3 cut(s)	502, 2128, 2439
BmeT110I	CYCGRG	3 cut(s)	233, 1302, 3630
BmgT120I	GGNCC	6 cut(s)	502, 1763, 1782, 2128, 2439, 3089
BmiI	GGNNCC	9 cut(s)	9, 108, 902, 1189, 1783, 1819, 2440, 3530, 3574
BmrFI	CCNGG	8 cut(s)	482, 709, 1734, 2691, 3266, 3296, 3631, 3632
BmrI	ACTGGG	1 cut(s)	855
BmsI	GCATC	7 cut(s)	160, 745, 1059, 1350, 1625, 2749, 2899
BmtI	GCTAGC	3 cut(s)	440, 795, 2297
BmuI	ACTGGG	1 cut(s)	855
BpiI	GAAGAC	2 cut(s)	291, 2883
BpmI	CTGGAG	3 cut(s)	2387, 2877, 3317
Bpu10I	CCTNAGC	3 cut(s)	963, 1068, 2451
BpuEI	CTTGAG	4 cut(s)	368, 1632, 2750, 2829
BpuMI	CCSGG	2 cut(s)	3631, 3632
Bsa29I	ATCGAT	1 cut(s)	257
BsaAI	YACGTR	2 cut(s)	2526, 2991
BsaBI	GATNNNNATC	2 cut(s)	636, 1801
BsaHI	GRCGYC	1 cut(s)	8
BsaWI	WCCGGW	1 cut(s)	203
BsaXI	ACNNNNNCTCC	2 cut(s)	21, 51
Bse3DI	GCAATG	5 cut(s)	891, 1279, 3121, 3184, 3241
Bse8I	GATNNNNATC	2 cut(s)	636, 1801
BseBI	CCWGG	6 cut(s)	482, 709, 1734, 2691, 3266, 3296
BseCI	ATCGAT	1 cut(s)	257
BseGI	GGATG	5 cut(s)	25, 717, 1496, 2065, 2506
BseJI	GATNNNNATC	2 cut(s)	636, 1801
BseMI	GCAATG	5 cut(s)	891, 1279, 3121, 3184, 3241
BseMII	CTCAG	7 cut(s)	776, 954, 1059, 2442, 2510, 2843, 2904
BseRI	GAGGAG	6 cut(s)	119, 506, 980, 2156, 2420, 2622
BseSI	GKGCMC	2 cut(s)	903, 1822
BseX3I	CGGCCG	1 cut(s)	224
BsgI	GTGCAG	6 cut(s)	589, 859, 1203, 2061, 2472, 2816
Bsh1236I	CGCG	1 cut(s)	318
Bsh1285I	CGRYCG	1 cut(s)	227
BshNI	GGYRCC	4 cut(s)	7, 900, 1817, 3572
BshVI	ATCGAT	1 cut(s)	257
BsiEI	CGRYCG	1 cut(s)	227
BsiHKAI	GWGCWC	6 cut(s)	101, 168, 457, 1311, 2460, 3370
BsiHKCI	CYCGRG	3 cut(s)	233, 1302, 3630
BsiSI	CCGG	2 cut(s)	204, 3631
BslFI	GGGAC	2 cut(s)	1715, 2452
BsmAI	GTCTC	2 cut(s)	1623, 3641
BsmFI	GGGAC	2 cut(s)	1715, 2452
BsmI	GAATGC	3 cut(s)	573, 2579, 3391
BsoBI	CYCGRG	3 cut(s)	233, 1302, 3630
Bsp1286I	GDGCHC	8 cut(s)	101, 168, 457, 903, 1311, 1822, 2460, 3370
Bsp1407I	TGTACA	1 cut(s)	446
Bsp19I	CCATGG	4 cut(s)	189, 605, 2474, 3126
BspCNI	CTCAG	7 cut(s)	775, 955, 1060, 2443, 2509, 2842, 2905
BspDI	ATCGAT	1 cut(s)	257
BspFNI	CGCG	1 cut(s)	318
BspLI	GGNNCC	9 cut(s)	9, 108, 902, 1189, 1783, 1819, 2440, 3530, 3574
BspOI	GCTAGC	3 cut(s)	440, 795, 2297
BspPI	GGATC	6 cut(s)	1791, 1866, 2220, 2673, 2765, 3625
BspQI	GCTCTTC	1 cut(s)	531
BspT107I	GGYRCC	4 cut(s)	7, 900, 1817, 3572
BspTI	CTTAAG	2 cut(s)	658, 1366
BsrDI	GCAATG	5 cut(s)	891, 1279, 3121, 3184, 3241
BsrGI	TGTACA	1 cut(s)	446
BssNI	GRCGYC	1 cut(s)	8
BssT1I	CCWWGG	6 cut(s)	189, 605, 1391, 2474, 2774, 3126
Bst2UI	CCWGG	6 cut(s)	482, 709, 1734, 2691, 3266, 3296
Bst6I	CTCTTC	4 cut(s)	531, 1658, 1998, 3296
BstACI	GRCGYC	1 cut(s)	8
BstAFI	CTTAAG	2 cut(s)	658, 1366
BstAPI	GCANNNNNTGC	5 cut(s)	1622, 3066, 3204, 3252, 3261
BstAUI	TGTACA	1 cut(s)	446
BstBAI	YACGTR	2 cut(s)	2526, 2991
BstDSI	CCRYGG	5 cut(s)	189, 605, 2442, 2474, 3126
BstENI	CCTNNNNNAGG	2 cut(s)	485, 2637
BstF5I	GGATG	5 cut(s)	25, 717, 1496, 2065, 2506
BstFNI	CGCG	1 cut(s)	318
BstH2I	RGCGCY	3 cut(s)	11, 241, 2099
BstHHI	GCGC	6 cut(s)	10, 175, 240, 2044, 2098, 3066
BstMAI	GTCTC	2 cut(s)	1623, 3641
BstMCI	CGRYCG	1 cut(s)	227
BstNI	CCWGG	6 cut(s)	482, 709, 1734, 2691, 3266, 3296
BstSCI	CCNGG	8 cut(s)	480, 707, 1732, 2689, 3264, 3294, 3629, 3630
BstSLI	GKGCMC	2 cut(s)	903, 1822
BstUI	CGCG	1 cut(s)	318
BstV2I	GAAGAC	2 cut(s)	291, 2883
BstX2I	RGATCY	1 cut(s)	3617
BstYI	RGATCY	1 cut(s)	3617
BstZI	CGGCCG	1 cut(s)	224
Bsu15I	ATCGAT	1 cut(s)	257
BsuI	GTATCC	1 cut(s)	2515
BsuTUI	ATCGAT	1 cut(s)	257
BtgI	CCRYGG	5 cut(s)	189, 605, 2442, 2474, 3126
BtsCI	GGATG	5 cut(s)	25, 717, 1496, 2065, 2506
BtsI	GCAGTG	1 cut(s)	1461
BtsIMutI	CAGTG	8 cut(s)	733, 1461, 1566, 1826, 2078, 2123, 2397, 3586
CaiI	CAGNNNCTG	2 cut(s)	565, 1460
CfoI	GCGC	6 cut(s)	10, 175, 240, 2044, 2098, 3066
Cfr13I	GGNCC	6 cut(s)	502, 1763, 1782, 2128, 2439, 3089
Cfr9I	CCCGGG	1 cut(s)	3630
ClaI	ATCGAT	1 cut(s)	257
CseI	GACGC	2 cut(s)	429, 3497
CsiI	ACCWGGT	1 cut(s)	2689
Csp6I	GTAC	5 cut(s)	447, 859, 873, 3136, 3573
CviQI	GTAC	5 cut(s)	447, 859, 873, 3136, 3573
DinI	GGCGCC	1 cut(s)	9
DraI	TTTAAA	1 cut(s)	1912
EaeI	YGGCCR	6 cut(s)	186, 224, 591, 2375, 2425, 3379
EagI	CGGCCG	1 cut(s)	224
Eam1104I	CTCTTC	4 cut(s)	531, 1658, 1998, 3296
EarI	CTCTTC	4 cut(s)	531, 1658, 1998, 3296
EciI	GGCGGA	1 cut(s)	42
Ecl136II	GAGCTC	3 cut(s)	166, 455, 3368
EclXI	CGGCCG	1 cut(s)	224
Eco130I	CCWWGG	6 cut(s)	189, 605, 1391, 2474, 2774, 3126
Eco24I	GRGCYC	3 cut(s)	168, 457, 3370
Eco32I	GATATC	1 cut(s)	2161
Eco47I	GGWCC	3 cut(s)	502, 2128, 2439
Eco47III	AGCGCT	1 cut(s)	239
Eco52I	CGGCCG	1 cut(s)	224
Eco53kI	GAGCTC	3 cut(s)	166, 455, 3368
Eco57I	CTGAAG	4 cut(s)	1062, 1149, 1297, 1434
Eco88I	CYCGRG	3 cut(s)	233, 1302, 3630
EcoICRI	GAGCTC	3 cut(s)	166, 455, 3368
EcoNI	CCTNNNNNAGG	2 cut(s)	485, 2637
EcoO109I	RGGNCCY	1 cut(s)	502
EcoRI	GAATTC	3 cut(s)	49, 1151, 1831
EcoRII	CCWGG	6 cut(s)	480, 707, 1732, 2689, 3264, 3294
EcoRV	GATATC	1 cut(s)	2161
EcoT14I	CCWWGG	6 cut(s)	189, 605, 1391, 2474, 2774, 3126
EcoT22I	ATGCAT	1 cut(s)	3393
EcoT38I	GRGCYC	3 cut(s)	168, 457, 3370
EgeI	GGCGCC	1 cut(s)	9
EheI	GGCGCC	1 cut(s)	9
ErhI	CCWWGG	6 cut(s)	189, 605, 1391, 2474, 2774, 3126
FalI	AAGNNNNNCTT	2 cut(s)	2082, 2114
FaqI	GGGAC	2 cut(s)	1715, 2452
FauI	CCCGC	1 cut(s)	3599
FauNDI	CATATG	1 cut(s)	810
FbaI	TGATCA	4 cut(s)	376, 1237, 2674, 3636
FokI	GGATG	5 cut(s)	32, 724, 1503, 2072, 2513
FriOI	GRGCYC	3 cut(s)	168, 457, 3370
FspBI	CTAG	8 cut(s)	437, 752, 792, 972, 1626, 2294, 2339, 2811
GlaI	GCGC	6 cut(s)	9, 174, 239, 2043, 2097, 3065
GsuI	CTGGAG	3 cut(s)	2387, 2877, 3317
HaeII	RGCGCY	3 cut(s)	11, 241, 2099
HapII	CCGG	2 cut(s)	204, 3631
HgaI	GACGC	2 cut(s)	429, 3497
HhaI	GCGC	6 cut(s)	10, 175, 240, 2044, 2098, 3066
Hin1I	GRCGYC	1 cut(s)	8
Hin6I	GCGC	6 cut(s)	8, 173, 238, 2042, 2096, 3064
HinP1I	GCGC	6 cut(s)	8, 173, 238, 2042, 2096, 3064
HincII	GTYRAC	5 cut(s)	15, 58, 733, 806, 1410
HindII	GTYRAC	5 cut(s)	15, 58, 733, 806, 1410
HindIII	AAGCTT	6 cut(s)	683, 1561, 1967, 2200, 2303, 2659
HpaI	GTTAAC	1 cut(s)	15
HpaII	CCGG	2 cut(s)	204, 3631
HphI	GGTGA	7 cut(s)	618, 697, 2180, 2491, 2885, 2886, 3646
HpyCH4IV	ACGT	3 cut(s)	1406, 2525, 2990
HpySE526I	ACGT	3 cut(s)	1406, 2525, 2990
Hsp92I	GRCGYC	1 cut(s)	8
HspAI	GCGC	6 cut(s)	8, 173, 238, 2042, 2096, 3064
KasI	GGCGCC	1 cut(s)	7
KpnI	GGTACC	1 cut(s)	3576
Ksp22I	TGATCA	4 cut(s)	376, 1237, 2674, 3636
KspAI	GTTAAC	1 cut(s)	15
LguI	GCTCTTC	1 cut(s)	531
LmnI	GCTCC	9 cut(s)	106, 163, 180, 452, 1187, 2179, 2368, 2407, 2463
LweI	GCATC	7 cut(s)	160, 745, 1059, 1350, 1625, 2749, 2899
MabI	ACCWGGT	1 cut(s)	2689
MaeI	CTAG	8 cut(s)	437, 752, 792, 972, 1626, 2294, 2339, 2811
MaeII	ACGT	3 cut(s)	1406, 2525, 2990
MfeI	CAATTG	2 cut(s)	1539, 3256
MflI	RGATCY	1 cut(s)	3617
MhlI	GDGCHC	8 cut(s)	101, 168, 457, 903, 1311, 1822, 2460, 3370
MlsI	TGGCCA	3 cut(s)	188, 2377, 3381
MluI	ACGCGT	1 cut(s)	316
MluNI	TGGCCA	3 cut(s)	188, 2377, 3381
Mly113I	GGCGCC	1 cut(s)	8
MlyI	GAGTC	3 cut(s)	2119, 2741, 2758
MmeI	TCCRAC	4 cut(s)	1381, 2157, 2675, 2866
Mox20I	TGGCCA	3 cut(s)	188, 2377, 3381
Mph1103I	ATGCAT	1 cut(s)	3393
MroXI	GAANNNNTTC	6 cut(s)	684, 918, 1105, 1173, 1281, 1684
MscI	TGGCCA	3 cut(s)	188, 2377, 3381
MseI	TTAA	9 cut(s)	14, 659, 939, 950, 1367, 1557, 1911, 2568, 2597
MslI	CAYNNNNRTG	9 cut(s)	276, 578, 710, 835, 1603, 2049, 3009, 3109, 3632
Msp20I	TGGCCA	3 cut(s)	188, 2377, 3381
MspA1I	CMGCKG	4 cut(s)	229, 1205, 2234, 2817
MspCI	CTTAAG	2 cut(s)	658, 1366
MspI	CCGG	2 cut(s)	204, 3631
MspR9I	CCNGG	8 cut(s)	482, 709, 1734, 2691, 3266, 3296, 3631, 3632
MunI	CAATTG	2 cut(s)	1539, 3256
Mva1269I	GAATGC	3 cut(s)	573, 2579, 3391
MvaI	CCWGG	6 cut(s)	482, 709, 1734, 2691, 3266, 3296
MvnI	CGCG	1 cut(s)	318
NarI	GGCGCC	1 cut(s)	8
NciI	CCSGG	2 cut(s)	3631, 3632
NcoI	CCATGG	4 cut(s)	189, 605, 2474, 3126
NdeI	CATATG	1 cut(s)	810
NheI	GCTAGC	3 cut(s)	436, 791, 2293
NlaIV	GGNNCC	9 cut(s)	9, 108, 902, 1189, 1783, 1819, 2440, 3530, 3574
NmeAIII	GCCGAG	3 cut(s)	619, 1791, 3529
NmuCI	GTSAC	4 cut(s)	1712, 2111, 2479, 3422
NsiI	ATGCAT	1 cut(s)	3393
OliI	CACNNNNGTG	1 cut(s)	3632
PaeI	GCATGC	5 cut(s)	834, 1127, 1500, 3064, 3484
PaeR7I	CTCGAG	2 cut(s)	233, 1302
PciI	ACATGT	1 cut(s)	735
PciSI	GCTCTTC	1 cut(s)	531
PcsI	WCGNNNNNNNCGW	1 cut(s)	107
PctI	GAATGC	3 cut(s)	573, 2579, 3391
PdmI	GAANNNNTTC	6 cut(s)	684, 918, 1105, 1173, 1281, 1684
PflFI	GACNNNGTC	1 cut(s)	1076
PflMI	CCANNNNNTGG	2 cut(s)	184, 481
PleI	GAGTC	3 cut(s)	2118, 2740, 2758
PluTI	GGCGCC	1 cut(s)	11
PpsI	GAGTC	3 cut(s)	2118, 2740, 2758
Ppu21I	YACGTR	2 cut(s)	2526, 2991
PpuMI	RGGWCCY	1 cut(s)	502
PscI	ACATGT	1 cut(s)	735
Psp124BI	GAGCTC	3 cut(s)	168, 457, 3370
Psp1406I	AACGTT	1 cut(s)	1406
Psp5II	RGGWCCY	1 cut(s)	502
Psp6I	CCWGG	6 cut(s)	480, 707, 1732, 2689, 3264, 3294
PspGI	CCWGG	6 cut(s)	480, 707, 1732, 2689, 3264, 3294
PspN4I	GGNNCC	9 cut(s)	9, 108, 902, 1189, 1783, 1819, 2440, 3530, 3574
PspPI	GGNCC	6 cut(s)	502, 1763, 1782, 2128, 2439, 3089
PspPPI	RGGWCCY	1 cut(s)	502
PspXI	VCTCGAGB	1 cut(s)	233
PsrI	GAACNNNNNNTAC	2 cut(s)	1501, 1533
PstNI	CAGNNNCTG	2 cut(s)	565, 1460
PsuI	RGATCY	1 cut(s)	3617
PsyI	GACNNNGTC	1 cut(s)	1076
PvuII	CAGCTG	1 cut(s)	2234
RsaI	GTAC	5 cut(s)	448, 860, 874, 3137, 3574
RsaNI	GTAC	5 cut(s)	447, 859, 873, 3136, 3573
RseI	CAYNNNNRTG	9 cut(s)	276, 578, 710, 835, 1603, 2049, 3009, 3109, 3632
SacI	GAGCTC	3 cut(s)	168, 457, 3370
SapI	GCTCTTC	1 cut(s)	531
SaqAI	TTAA	9 cut(s)	14, 659, 939, 950, 1367, 1557, 1911, 2568, 2597
Sau96I	GGNCC	6 cut(s)	502, 1763, 1782, 2128, 2439, 3089
ScaI	AGTACT	3 cut(s)	860, 874, 3137
SchI	GAGTC	3 cut(s)	2119, 2741, 2758
ScrFI	CCNGG	8 cut(s)	482, 709, 1734, 2691, 3266, 3296, 3631, 3632
SduI	GDGCHC	8 cut(s)	101, 168, 457, 903, 1311, 1822, 2460, 3370
SexAI	ACCWGGT	1 cut(s)	2689
SfaNI	GCATC	7 cut(s)	160, 745, 1059, 1350, 1625, 2749, 2899
SfoI	GGCGCC	1 cut(s)	9
Sfr274I	CTCGAG	2 cut(s)	233, 1302
SinI	GGWCC	3 cut(s)	502, 2128, 2439
SlaI	CTCGAG	2 cut(s)	233, 1302
SmaI	CCCGGG	1 cut(s)	3632
SmiMI	CAYNNNNRTG	9 cut(s)	276, 578, 710, 835, 1603, 2049, 3009, 3109, 3632
SmlI	CTYRAG	8 cut(s)	233, 347, 658, 1302, 1366, 1647, 2729, 2844
SmoI	CTYRAG	8 cut(s)	233, 347, 658, 1302, 1366, 1647, 2729, 2844
SphI	GCATGC	5 cut(s)	834, 1127, 1500, 3064, 3484
SspDI	GGCGCC	1 cut(s)	7
SspI	AATATT	1 cut(s)	1174
SspMI	CTAG	8 cut(s)	437, 752, 792, 972, 1626, 2294, 2339, 2811
SstI	GAGCTC	3 cut(s)	168, 457, 3370
StyD4I	CCNGG	8 cut(s)	480, 707, 1732, 2689, 3264, 3294, 3629, 3630
StyI	CCWWGG	6 cut(s)	189, 605, 1391, 2474, 2774, 3126
TaiI	ACGT	3 cut(s)	1409, 2528, 2993
TaqI	TCGA	8 cut(s)	47, 168, 234, 257, 1303, 2905, 3364, 3565
TaqII	GACCGA	1 cut(s)	67
TatI	WGTACW	4 cut(s)	446, 858, 872, 3135
TauI	GCSGC	4 cut(s)	8, 229, 2430, 2817
Tru1I	TTAA	9 cut(s)	14, 659, 939, 950, 1367, 1557, 1911, 2568, 2597
Tru9I	TTAA	9 cut(s)	14, 659, 939, 950, 1367, 1557, 1911, 2568, 2597
TscAI	CASTG	8 cut(s)	733, 1461, 1573, 1833, 2085, 2130, 2404, 3593
TseFI	GTSAC	4 cut(s)	1712, 2111, 2479, 3422
Tsp45I	GTSAC	4 cut(s)	1712, 2111, 2479, 3422
TspGWI	ACGGA	4 cut(s)	32, 43, 116, 2699
TspMI	CCCGGG	1 cut(s)	3630
TspRI	CASTG	8 cut(s)	733, 1461, 1573, 1833, 2085, 2130, 2404, 3593
Tth111I	GACNNNGTC	1 cut(s)	1076
Van91I	CCANNNNNTGG	2 cut(s)	184, 481
Vha464I	CTTAAG	2 cut(s)	658, 1366
VpaK11BI	GGWCC	3 cut(s)	502, 2128, 2439
XagI	CCTNNNNNAGG	2 cut(s)	485, 2637
XbaI	TCTAGA	1 cut(s)	751
XhoI	CTCGAG	2 cut(s)	233, 1302
XmaI	CCCGGG	1 cut(s)	3630
XmnI	GAANNNNTTC	6 cut(s)	684, 918, 1105, 1173, 1281, 1684
XspI	CTAG	8 cut(s)	437, 752, 792, 972, 1626, 2294, 2339, 2811
ZrmI	AGTACT	3 cut(s)	860, 874, 3137
Zsp2I	ATGCAT	1 cut(s)	3393

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.