Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002376

Physical Location & Seq

Genomic Coordinates Reverse (-)

30909237 .. 30917083

Gene Structure 7847 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1228100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3684 bp

ATGGCGACGCCGTTAACGGACGGAGGCGGAGGACTGGCGATGCTGTCGAATTCCGTCAACAAGGTGGATTCTTCGTCGGTCAATGGATGCTTGGCGAGCTCGGAGGAGCCAACGAGGTCTCCGATTCTGATTTTCTTGTTCTTTCACAAGGCAATTCGGAAGGAGCTCGATGCGCTTCACCGATTGGCCATGGCCTTCGCCACCGGAAAAAGAACCGATATTCAGCCGCTGCTCGAGCGCTACCATTTCTTGAGATCAATTTACAAGCACCACTCCAATGCCGAAGACGAGGTAATCTTTCCAGCTCTTGATATACGGGTGAAGAATGTAGCACAAACTTACTCCCTTGAACATGAGGGTGAAACAAATCTTTTTGATCATCTATTTGAGCTGCTAAGTTCAAATGCCAAAAATGATGAAAACTTCCCGAGAGAGCTAGCGTCTTGTACAGGAGCCCTACAAACATCAATTAGCCAACACATGGCAAAGGAAGAGGAACAGGTCTTTCCGTTGCTCATTGAGAAATTCTCAGTTGCGGAGCAGGCAGCTCTGGTCTGGCAGTTTCTGTGCAGCATTCCTGTGAATATGATGGCCGAGTTTCTTCCATGGCTTTCATCTTCTGTTTCACCTGATGAACATCAGGATTTACGCAAATGCTTAAGCAAGATAGTTCCAGGGGAAAAGCTTCTTCAACAAGTCATTTTCACCTGGATGGGAGGTAGAAGTAGTGCTGACATGTTCAGAAGTTCTATAGATACCCCTCAGTTCCAATGTTGTGTGGATTTTGGTGCTAGCACATCAAGTCAACATATGGAGAAGGCAAATCCTGCATGTGAGTGCAGGACTGGGAAAAGGAAGTATGTAGAGTCAAGTACTGATGTTTCTGATACCATTGAGGGGCACCCAATAAATGAAATACTTCTTTGGCATAATGCCATTAAAAGGGAGTTAAATGAGATAGCTGAGGAGGCTAGGAAGATACAACAGGCTGGAGATTTTACTAATCTATCAGCTTTCAACGAGAGGTTGCAATTTATCGCTGAAGTTTGCATCTTTCACAGTATTGCTGAGGACAAGGTCATATTTCCTGCCGTTGATGGAAAAATTTCATTTTTCCAAGAGCATGCTGAAGAAGAAAGCCAATTCAACGAAATTAGGTGTTTGATTGAAAATATTCAAAGTGCTGGAGCTATCTCTACTTCTGCTGATTTTTATGCAAAATTATGTTCTCATGCTGATCAAATAATGGAAACTATCCAGCGGCACTTTAGCAACGAAGAAGTTCAGGTTCTTCCACTTGCTCGAAAGCACTTCAGCTTCAAAAGGCAGCGTGAACTTTTGTATCAAGCTTTGTGTATGATGCCCTTAAGACTGATTGAGCGTGTATTGCCATGGCTAATCGGAAAGTTGACTGAAGATGAAGCAAATAATTTTCTCAAAAACATGCAGTTAGCAGCTCCAGTGCCTGATTCCGCTTTGGTAACACTCTTTTCGGGATGGGCATGCAAGGCTCGTAATCAGGGTTCATGTTTGTCTTCAAGTGCAATTGGTTTCTGTCCTGTTAAAAGCTTCACTGATATTGAAGAAGGTTTTGTTCATTCAGCATGTGCTTGTTCTTCTGCATCATCTGCTAGAGACAGCTTGAGACTTCAAGCAAATAATGTGAAGAGGCTAGCCAAACACAATGTTCCTATGTCATGCAAAGACAATGGTAGTGACCAGTCCTGCTGTGTCCCAGGTTTAGGAGTGAACAGCAATAATCTTGGGCCGAGTTCTCTTTTTGGGGCCAAGTCTTTGCGCTCCTTATCTTTCAGTTCTGGTGCCCCATCTCTTAATTCCAGTCTTTTTGTCTGGGAAACGGATAATTCTTCTTCTGATATTGGCTGTGGAGAACGCCCCATTGATACTATTTTTAAATTTCATAAAGCCATACGTAAAGATTTGGAGTATTTAGATATTGAATCTGGAAAGCTTATTTATTGTGATGAAACCACTCTTCGGCAGTTCATAGGAAGGTTTCGTTTGCTGTGGGGCTTATACAGAGCGCATAGTAATGCGGAGGATGATATAGTGTTTCCTGCGCTGGAATCCAAGGAGGCACTTCATAATGTCAGTCACTCATACACACTGGACCATAAACAGGAGGAAAAATTATTTGAAGACATCTCATGTGTTCTTTCAGAACTTTCTCACCTTCATGAAAGCTTGCAAAAGGATGACATGGATGAGGATTTAGTTGGAAGTAGCATTAGCTTTTCTGCTGCTAATGGTAACTACTCTAAAAAGTATGACAAGCTAGCTACAAAGCTTCAAGGAATGTGCAAATCTATCAAAGTTACACTAGATCAGCATATTTTCAGGGAAGAACTTGAGCTGTGGCCATTATTCGGCAGACACTTCACTGTGGAGGAGCAAGACAAAATAGTTGGCCGCATTATTGGGACCACAGGTGCTGAGGTGCTCCAATCGATGTTACCATGGGTAACTTCTGCACTTACTCAGGATGAACAGAACAAAATGATGGATACATGGAAGCAAGCAACTAAAAATACAATGTTTCACGAGTGGCTTAACGAATGCTGGAAAGGAATTTCAGAGTCAACTTCACAGACTGAAACATCGGAAAGTAGCACTCCTCAAAAAGGTGTTGAATTTCAAGAAAGCTTGGACCAGCCCGATCAGATGTTCAAACCTGGTTGGAAGGATATTTTCCGTATGAATCAAAATGAACTTGAGTCAGAGATCAGAAAGGTTTATCGCGATGCAACTCTTGATCCGAGGAGAAAGGCATATCTAGTGCAGAATCTTATGACTAGCCGCTGGATAGCTACTCAGCAGAAGTTGCCTCAAGAAATAGCAGGAGAATCTTCTAATGGTGAAGACATGGTTGGACACTCACCAGCATATCGATGTGCAGAGAAAAAAGAGTTTGGGTGCGAACACTATAAAAGAAACTGCAAACTCCGAGCTGCTTGCTGTGGCAAGTTATTTACTTGTAGATTTTGCCATGACAATGTGAGCGATCATTCAATGGATAGGAAAGCAACAACTGAAATGATGTGCATGCGCTGCCTGACCGTTCAGCCGGTTGGGCCAATATGCACTACACCTTCATGCAATGAACTATCCATGGCAAAGTACTATTGCAATATATGCAAATTTTTTGATGATGAAAGGACTGTGTATCACTGCCCATTTTGCAATTTATGCCGACTCGGAAAGGGTCTTGGCATTGACTATTTTCATTGCATGACATGCAATTGTTGCCTGGGGATAAAGTTAGTGAACCACAAGTGCTTGGAGAAGAGTTTAGAAACAAATTGCCCCATCTGCTGTGATTTTTTGTTCACATCAAGTGCAACGGTTCGAGCTCTGCCCTGTGGCCACTACATGCATTCTGCTTGCTTTCAGGCGTACACTTGCAGTCACTATACTTGTCCAATTTGCAGCAAATCTATGGGAGATATGGCGGCTTACTTTGGCATGCTTGATGCATTATTGGCTGCTGAGCAGCTTCCAGAGGAATACAGGAATCGTTGTCAGGACATTCTGTGTAATGACTGTGATCGAAAGGGTACCTCACGGTTTCACTGGCTATATCACAAGTGCGGAAACTGCGGATCTTACAACACCCGGGTGATCAAAGGCGAGACATCAAATACCGACTGCCCGACATCAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hemerythrin zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

1228

Amino Acids

138.37

Weight (kDa)

5.82

Isoelectric Point (pI)

50.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hemerythrin	PF01814	47 - 172	1.8e-11	Hemerythrin HHE cation binding domain
Hemerythrin	PF01814	302 - 434	2.1e-06	Hemerythrin HHE cation binding domain
zf-CHY	PF05495	979 - 1047	1.2e-19	CHY zinc finger
zf-RING_2	PF13639	1107 - 1149	4.7e-07	Ring finger domain
zinc_ribbon_6	PF14599	1155 - 1212	5.1e-24	Zinc-ribbon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18290
fragaria_vesca	FvH4_4g26990 FvH4_4g26990 FvH4_4g26990 FvH4_4g26990 FvH4_5g11860 FvH4_5g11860 FvH4_5g11860
malus_domestica	MD06G1217900.v1.1 MD14G1228100.v1.1 MD16G1039900.v1.1
prunus_persica	Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1
pyrus_communis	pycom06g19410 pycom14g18950 pycom16g03460
rosa_chinensis	RchiOBHm_Chr4g0434791 RchiOBHm_Chr7g0185731
rosa_laevigata	RLG00000004888 RLG00000006649
rosa_multiflora	Rmu_sc0000179.1_g000001 Rmu_sc0002952.1_g000002
rosa_roxburghii	Rroxscaffold_3G00268670 Rroxscaffold_5G00376000
rosa_rugosa	Rorug04G0278300 Rorug04G0278400 Rorug06G0470900
rosa_samantha	Rh4AG334900 Rh4CG357800 Rh4DG337300 Rh7AG071600 Rh7BG076200 Rh7DG076200
rosa_wichuraiana	Rw4G029200 Rw7G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	3575
AccB1I	GGYRCC	3 cut(s)	900, 1820, 3575
AccB7I	CCANNNNNTGG	1 cut(s)	481
AccII	CGCG	1 cut(s)	2760
AclWI	GGATC	2 cut(s)	2768, 3628
AcoI	YGGCCR	5 cut(s)	186, 591, 2378, 2428, 3382
AcsI	RAATTY	7 cut(s)	49, 524, 1104, 1916, 2589, 2651, 3158
AcuI	CTGAAG	4 cut(s)	1062, 1149, 1297, 1434
AcyI	GRCGYC	1 cut(s)	8
AfaI	GTAC	5 cut(s)	448, 874, 3140, 3416, 3577
AfeI	AGCGCT	1 cut(s)	239
AfiI	CCNNNNNNNGG	8 cut(s)	481, 897, 942, 1742, 1998, 2140, 2387, 2642
AflII	CTTAAG	2 cut(s)	658, 1366
AflIII	ACRYGT	1 cut(s)	735
AjnI	CCWGG	5 cut(s)	673, 707, 1735, 2692, 3267
AjuI	GAANNNNNNNTTGG	2 cut(s)	2871, 2903
AleI	CACNNNNGTG	1 cut(s)	3635
Alw21I	GWGCWC	4 cut(s)	101, 168, 2463, 3373
Alw26I	GTCTC	4 cut(s)	123, 1629, 1639, 3644
AlwI	GGATC	2 cut(s)	2768, 3628
AlwNI	CAGNNNCTG	4 cut(s)	229, 565, 1466, 2453
Ama87I	CYCGRG	3 cut(s)	233, 427, 3633
Aor51HI	AGCGCT	1 cut(s)	239
AoxI	GGCC	9 cut(s)	186, 192, 591, 1766, 1785, 2378, 2428, 3092, 3382
ApoI	RAATTY	7 cut(s)	49, 524, 1104, 1916, 2589, 2651, 3158
Asp700I	GAANNNNTTC	5 cut(s)	684, 918, 1105, 1173, 1281
Asp718I	GGTACC	1 cut(s)	3575
AspLEI	GCGC	6 cut(s)	175, 240, 1800, 2047, 2083, 3069
AspS9I	GGNCC	6 cut(s)	1766, 1785, 2131, 2442, 2668, 3092
AsuC2I	CCSGG	2 cut(s)	3634, 3635
AsuHPI	GGTGA	9 cut(s)	170, 331, 371, 618, 697, 2183, 2888, 2889, 3649
AsuNHI	GCTAGC	4 cut(s)	436, 791, 1672, 2296
AvaI	CYCGRG	3 cut(s)	233, 427, 3633
AvaII	GGWCC	3 cut(s)	2131, 2442, 2668
BaeGI	GKGCMC	2 cut(s)	903, 1825
BaeI	ACNNNNGTAYC	2 cut(s)	1325, 1358
BalI	TGGCCA	3 cut(s)	188, 2380, 3384
BanI	GGYRCC	3 cut(s)	900, 1820, 3575
BanII	GRGCYC	4 cut(s)	101, 168, 457, 3373
BauI	CACGAG	1 cut(s)	2561
BbsI	GAAGAC	4 cut(s)	291, 1527, 2166, 2886
Bbv12I	GWGCWC	4 cut(s)	101, 168, 2463, 3373
BbvCI	CCTCAGC	3 cut(s)	963, 1068, 2454
BccI	CCATC	7 cut(s)	583, 706, 1091, 1491, 1834, 2515, 3335
BceAI	ACGGC	1 cut(s)	1076
BcgI	CGANNNNNNTGC	2 cut(s)	2955, 2989
BciT130I	CCWGG	5 cut(s)	675, 709, 1737, 2694, 3269
BciVI	GTATCC	1 cut(s)	2518
BclI	TGATCA	3 cut(s)	376, 1237, 3639
BcnI	CCSGG	2 cut(s)	3634, 3635
BcoDI	GTCTC	4 cut(s)	123, 1629, 1639, 3644
BfaI	CTAG	9 cut(s)	437, 792, 972, 1632, 1673, 2297, 2342, 2795, 2814
BfmI	CTRYAG	1 cut(s)	750
BfoI	RGCGCY	1 cut(s)	241
BfrI	CTTAAG	2 cut(s)	658, 1366
BfuI	GTATCC	1 cut(s)	2518
BglI	GCCNNNNNGGC	1 cut(s)	3091
BlpI	GCTNAGC	1 cut(s)	3507
BmcAI	AGTACT	2 cut(s)	874, 3140
Bme1390I	CCNGG	7 cut(s)	675, 709, 1737, 2694, 3269, 3634, 3635
Bme18I	GGWCC	3 cut(s)	2131, 2442, 2668
BmeT110I	CYCGRG	3 cut(s)	233, 427, 3633
BmgT120I	GGNCC	6 cut(s)	1766, 1785, 2131, 2442, 2668, 3092
BmiI	GGNNCC	7 cut(s)	108, 454, 902, 1786, 1822, 2443, 3577
BmrFI	CCNGG	7 cut(s)	675, 709, 1737, 2694, 3269, 3634, 3635
BmrI	ACTGGG	1 cut(s)	855
BmsI	GCATC	8 cut(s)	30, 77, 160, 1059, 1350, 1631, 2752, 3481
BmtI	GCTAGC	4 cut(s)	440, 795, 1676, 2300
BmuI	ACTGGG	1 cut(s)	855
BpiI	GAAGAC	4 cut(s)	291, 1527, 2166, 2886
BplI	GAGNNNNNCTC	2 cut(s)	512, 544
BpmI	CTGGAG	3 cut(s)	1011, 1206, 1443
Bpu10I	CCTNAGC	3 cut(s)	963, 1068, 2454
Bpu1102I	GCTNAGC	1 cut(s)	3507
BpuEI	CTTGAG	5 cut(s)	271, 1663, 2390, 2753, 2832
BpuMI	CCSGG	2 cut(s)	3634, 3635
Bsa29I	ATCGAT	2 cut(s)	2468, 2908
BsaAI	YACGTR	1 cut(s)	1934
BsaBI	GATNNNNATC	1 cut(s)	636
BsaHI	GRCGYC	1 cut(s)	8
BsaI	GGTCTC	1 cut(s)	123
BsaWI	WCCGGW	1 cut(s)	203
BsaXI	ACNNNNNCTCC	2 cut(s)	103, 133
Bsc4I	CCNNNNNNNGG	8 cut(s)	481, 897, 942, 1742, 1998, 2140, 2387, 2642
Bse118I	RCCGGY	1 cut(s)	3085
Bse1I	ACTGG	7 cut(s)	39, 850, 1460, 1720, 1839, 2133, 3596
Bse3DI	GCAATG	2 cut(s)	3124, 3244
Bse8I	GATNNNNATC	1 cut(s)	636
BseBI	CCWGG	5 cut(s)	675, 709, 1737, 2694, 3269
BseCI	ATCGAT	2 cut(s)	2468, 2908
BseGI	GGATG	7 cut(s)	92, 717, 1502, 2068, 2221, 2230, 2509
BseJI	GATNNNNATC	1 cut(s)	636
BseLI	CCNNNNNNNGG	8 cut(s)	481, 897, 942, 1742, 1998, 2140, 2387, 2642
BseMI	GCAATG	2 cut(s)	3124, 3244
BseMII	CTCAG	8 cut(s)	543, 776, 954, 1059, 2445, 2513, 2846, 3498
BseNI	ACTGG	7 cut(s)	39, 850, 1460, 1720, 1839, 2133, 3596
BseRI	GAGGAG	5 cut(s)	119, 980, 2423, 2625, 2794
BseSI	GKGCMC	2 cut(s)	903, 1825
BsgI	GTGCAG	5 cut(s)	589, 859, 2475, 2819, 2934
Bsh1236I	CGCG	1 cut(s)	2760
BshFI	GGCC	9 cut(s)	188, 194, 593, 1768, 1787, 2380, 2430, 3094, 3384
BshNI	GGYRCC	3 cut(s)	900, 1820, 3575
BshVI	ATCGAT	2 cut(s)	2468, 2908
BsiHKAI	GWGCWC	4 cut(s)	101, 168, 2463, 3373
BsiHKCI	CYCGRG	3 cut(s)	233, 427, 3633
BsiSI	CCGG	3 cut(s)	204, 3086, 3634
BslFI	GGGAC	2 cut(s)	1718, 2455
BslI	CCNNNNNNNGG	8 cut(s)	481, 897, 942, 1742, 1998, 2140, 2387, 2642
BsmAI	GTCTC	4 cut(s)	123, 1629, 1639, 3644
BsmFI	GGGAC	2 cut(s)	1718, 2455
BsmI	GAATGC	3 cut(s)	573, 2582, 3394
BsnI	GGCC	9 cut(s)	188, 194, 593, 1768, 1787, 2380, 2430, 3094, 3384
Bso31I	GGTCTC	1 cut(s)	123
BsoBI	CYCGRG	3 cut(s)	233, 427, 3633
Bsp1286I	GDGCHC	7 cut(s)	101, 168, 457, 903, 1825, 2463, 3373
Bsp1407I	TGTACA	1 cut(s)	446
Bsp1720I	GCTNAGC	1 cut(s)	3507
Bsp19I	CCATGG	5 cut(s)	189, 605, 1391, 2477, 3129
Bsp68I	TCGCGA	1 cut(s)	2760
BspANI	GGCC	9 cut(s)	188, 194, 593, 1768, 1787, 2380, 2430, 3094, 3384
BspCNI	CTCAG	8 cut(s)	542, 775, 955, 1060, 2446, 2512, 2845, 3499
BspDI	ATCGAT	2 cut(s)	2468, 2908
BspFNI	CGCG	1 cut(s)	2760
BspHI	TCATGA	1 cut(s)	2197
BspLI	GGNNCC	7 cut(s)	108, 454, 902, 1786, 1822, 2443, 3577
BspOI	GCTAGC	4 cut(s)	440, 795, 1676, 2300
BspPI	GGATC	2 cut(s)	2768, 3628
BspT107I	GGYRCC	3 cut(s)	900, 1820, 3575
BspTI	CTTAAG	2 cut(s)	658, 1366
BspTNI	GGTCTC	1 cut(s)	123
BsrDI	GCAATG	2 cut(s)	3124, 3244
BsrFI	RCCGGY	1 cut(s)	3085
BsrGI	TGTACA	1 cut(s)	446
BsrI	ACTGG	7 cut(s)	39, 850, 1460, 1720, 1839, 2133, 3596
BssAI	RCCGGY	1 cut(s)	3085
BssNI	GRCGYC	1 cut(s)	8
BssSI	CACGAG	1 cut(s)	2561
BssT1I	CCWWGG	6 cut(s)	189, 605, 1391, 2091, 2477, 3129
Bst2BI	CACGAG	1 cut(s)	2561
Bst2UI	CCWGG	5 cut(s)	675, 709, 1737, 2694, 3269
Bst4CI	ACNGT	7 cut(s)	1061, 2404, 3079, 3181, 3364, 3563, 3585
Bst6I	CTCTTC	4 cut(s)	486, 1661, 2001, 3299
BstACI	GRCGYC	1 cut(s)	8
BstAFI	CTTAAG	2 cut(s)	658, 1366
BstAPI	GCANNNNNTGC	8 cut(s)	827, 1628, 2842, 3069, 3153, 3207, 3255, 3264
BstAUI	TGTACA	1 cut(s)	446
BstBAI	YACGTR	1 cut(s)	1934
BstDEI	CTNAG	9 cut(s)	395, 529, 762, 963, 1068, 2454, 2499, 2832, 3507
BstDSI	CCRYGG	5 cut(s)	189, 605, 1391, 2477, 3129
BstENI	CCTNNNNNAGG	1 cut(s)	2640
BstF5I	GGATG	7 cut(s)	92, 717, 1502, 2068, 2221, 2230, 2509
BstFNI	CGCG	1 cut(s)	2760
BstH2I	RGCGCY	1 cut(s)	241
BstHHI	GCGC	6 cut(s)	175, 240, 1800, 2047, 2083, 3069
BstMAI	GTCTC	4 cut(s)	123, 1629, 1639, 3644
BstNI	CCWGG	5 cut(s)	675, 709, 1737, 2694, 3269
BstSCI	CCNGG	7 cut(s)	673, 707, 1735, 2692, 3267, 3632, 3633
BstSFI	CTRYAG	1 cut(s)	750
BstSLI	GKGCMC	2 cut(s)	903, 1825
BstSNI	TACGTA	1 cut(s)	1934
BstUI	CGCG	1 cut(s)	2760
BstV2I	GAAGAC	4 cut(s)	291, 1527, 2166, 2886
BstX2I	RGATCY	1 cut(s)	3620
BstYI	RGATCY	1 cut(s)	3620
Bsu15I	ATCGAT	2 cut(s)	2468, 2908
BsuI	GTATCC	1 cut(s)	2518
BsuRI	GGCC	9 cut(s)	188, 194, 593, 1768, 1787, 2380, 2430, 3094, 3384
BsuTUI	ATCGAT	2 cut(s)	2468, 2908
BtgI	CCRYGG	5 cut(s)	189, 605, 1391, 2477, 3129
BtgZI	GCGATG	2 cut(s)	53, 2775
BtsCI	GGATG	7 cut(s)	92, 717, 1502, 2068, 2221, 2230, 2509
BtsI	GCAGTG	1 cut(s)	3187
BtsIMutI	CAGTG	6 cut(s)	1467, 1572, 2126, 2400, 3187, 3589
BtuMI	TCGCGA	1 cut(s)	2760
CaiI	CAGNNNCTG	4 cut(s)	229, 565, 1466, 2453
CciI	TCATGA	1 cut(s)	2197
CfoI	GCGC	6 cut(s)	175, 240, 1800, 2047, 2083, 3069
Cfr10I	RCCGGY	1 cut(s)	3085
Cfr13I	GGNCC	6 cut(s)	1766, 1785, 2131, 2442, 2668, 3092
Cfr9I	CCCGGG	1 cut(s)	3633
ClaI	ATCGAT	2 cut(s)	2468, 2908
CseI	GACGC	2 cut(s)	16, 429
CsiI	ACCWGGT	1 cut(s)	2692
Csp6I	GTAC	5 cut(s)	447, 873, 3139, 3415, 3576
CviQI	GTAC	5 cut(s)	447, 873, 3139, 3415, 3576
DdeI	CTNAG	9 cut(s)	395, 529, 762, 963, 1068, 2454, 2499, 2832, 3507
DraI	TTTAAA	1 cut(s)	1915
EaeI	YGGCCR	5 cut(s)	186, 591, 2378, 2428, 3382
Eam1104I	CTCTTC	4 cut(s)	486, 1661, 2001, 3299
EarI	CTCTTC	4 cut(s)	486, 1661, 2001, 3299
EciI	GGCGGA	1 cut(s)	42
Ecl136II	GAGCTC	3 cut(s)	99, 166, 3371
Eco105I	TACGTA	1 cut(s)	1934
Eco130I	CCWWGG	6 cut(s)	189, 605, 1391, 2091, 2477, 3129
Eco24I	GRGCYC	4 cut(s)	101, 168, 457, 3373
Eco31I	GGTCTC	1 cut(s)	123
Eco47I	GGWCC	3 cut(s)	2131, 2442, 2668
Eco47III	AGCGCT	1 cut(s)	239
Eco53kI	GAGCTC	3 cut(s)	99, 166, 3371
Eco57I	CTGAAG	4 cut(s)	1062, 1149, 1297, 1434
Eco88I	CYCGRG	3 cut(s)	233, 427, 3633
EcoICRI	GAGCTC	3 cut(s)	99, 166, 3371
EcoNI	CCTNNNNNAGG	1 cut(s)	2640
EcoRI	GAATTC	1 cut(s)	49
EcoRII	CCWGG	5 cut(s)	673, 707, 1735, 2692, 3267
EcoT14I	CCWWGG	6 cut(s)	189, 605, 1391, 2091, 2477, 3129
EcoT22I	ATGCAT	2 cut(s)	3396, 3496
EcoT38I	GRGCYC	4 cut(s)	101, 168, 457, 3373
ErhI	CCWWGG	6 cut(s)	189, 605, 1391, 2091, 2477, 3129
FaqI	GGGAC	2 cut(s)	1718, 2455
FauNDI	CATATG	1 cut(s)	810
FbaI	TGATCA	3 cut(s)	376, 1237, 3639
FokI	GGATG	7 cut(s)	99, 724, 1509, 2075, 2228, 2237, 2516
FriOI	GRGCYC	4 cut(s)	101, 168, 457, 3373
FspBI	CTAG	9 cut(s)	437, 792, 972, 1632, 1673, 2297, 2342, 2795, 2814
GlaI	GCGC	6 cut(s)	174, 239, 1799, 2046, 2082, 3068
GsuI	CTGGAG	3 cut(s)	1011, 1206, 1443
HaeII	RGCGCY	1 cut(s)	241
HaeIII	GGCC	9 cut(s)	188, 194, 593, 1768, 1787, 2380, 2430, 3094, 3384
HapII	CCGG	3 cut(s)	204, 3086, 3634
HgaI	GACGC	2 cut(s)	16, 429
HhaI	GCGC	6 cut(s)	175, 240, 1800, 2047, 2083, 3069
Hin1I	GRCGYC	1 cut(s)	8
Hin6I	GCGC	6 cut(s)	173, 238, 1798, 2045, 2081, 3067
HinP1I	GCGC	6 cut(s)	173, 238, 1798, 2045, 2081, 3067
HincII	GTYRAC	5 cut(s)	15, 58, 806, 1410, 2601
HindII	GTYRAC	5 cut(s)	15, 58, 806, 1410, 2601
HindIII	AAGCTT	7 cut(s)	683, 1347, 1567, 1970, 2203, 2306, 2662
HpaI	GTTAAC	1 cut(s)	15
HpaII	CCGG	3 cut(s)	204, 3086, 3634
HphI	GGTGA	9 cut(s)	170, 331, 371, 618, 697, 2183, 2888, 2889, 3649
Hpy99I	CGWCG	2 cut(s)	10, 79
HpyAV	CCTTC	8 cut(s)	154, 205, 811, 1580, 2007, 2204, 2695, 3120
HpyCH4III	ACNGT	7 cut(s)	1061, 2404, 3079, 3181, 3364, 3563, 3585
HpyCH4IV	ACGT	1 cut(s)	1933
HpyF3I	CTNAG	9 cut(s)	395, 529, 762, 963, 1068, 2454, 2499, 2832, 3507
HpySE526I	ACGT	1 cut(s)	1933
Hsp92I	GRCGYC	1 cut(s)	8
HspAI	GCGC	6 cut(s)	173, 238, 1798, 2045, 2081, 3067
KpnI	GGTACC	1 cut(s)	3579
Ksp22I	TGATCA	3 cut(s)	376, 1237, 3639
KspAI	GTTAAC	1 cut(s)	15
LmnI	GCTCC	9 cut(s)	106, 163, 452, 538, 1187, 1462, 1805, 2410, 2466
LweI	GCATC	8 cut(s)	30, 77, 160, 1059, 1350, 1631, 2752, 3481
MabI	ACCWGGT	1 cut(s)	2692
MaeI	CTAG	9 cut(s)	437, 792, 972, 1632, 1673, 2297, 2342, 2795, 2814
MaeII	ACGT	1 cut(s)	1933
MaeIII	GTNAC	8 cut(s)	1480, 1715, 2114, 2270, 2335, 2472, 2482, 3425
MfeI	CAATTG	2 cut(s)	1545, 3259
MflI	RGATCY	1 cut(s)	3620
MhlI	GDGCHC	7 cut(s)	101, 168, 457, 903, 1825, 2463, 3373
MlsI	TGGCCA	3 cut(s)	188, 2380, 3384
MluNI	TGGCCA	3 cut(s)	188, 2380, 3384
MlyI	GAGTC	4 cut(s)	875, 2606, 2744, 3207
MmeI	TCCRAC	3 cut(s)	2218, 2678, 2869
Mox20I	TGGCCA	3 cut(s)	188, 2380, 3384
Mph1103I	ATGCAT	2 cut(s)	3396, 3496
MroXI	GAANNNNTTC	5 cut(s)	684, 918, 1105, 1173, 1281
MscI	TGGCCA	3 cut(s)	188, 2380, 3384
MseI	TTAA	9 cut(s)	14, 659, 939, 950, 1367, 1563, 1833, 1914, 2571
Msp20I	TGGCCA	3 cut(s)	188, 2380, 3384
MspA1I	CMGCKG	3 cut(s)	229, 1261, 2820
MspCI	CTTAAG	2 cut(s)	658, 1366
MspI	CCGG	3 cut(s)	204, 3086, 3634
MspR9I	CCNGG	7 cut(s)	675, 709, 1737, 2694, 3269, 3634, 3635
MunI	CAATTG	2 cut(s)	1545, 3259
Mva1269I	GAATGC	3 cut(s)	573, 2582, 3394
MvaI	CCWGG	5 cut(s)	675, 709, 1737, 2694, 3269
MvnI	CGCG	1 cut(s)	2760
NciI	CCSGG	2 cut(s)	3634, 3635
NcoI	CCATGG	5 cut(s)	189, 605, 1391, 2477, 3129
NdeI	CATATG	1 cut(s)	810
NheI	GCTAGC	4 cut(s)	436, 791, 1672, 2296
NlaIV	GGNNCC	7 cut(s)	108, 454, 902, 1786, 1822, 2443, 3577
NmeAIII	GCCGAG	2 cut(s)	619, 1794
NmuCI	GTSAC	3 cut(s)	1715, 2114, 3425
NruI	TCGCGA	1 cut(s)	2760
NsiI	ATGCAT	2 cut(s)	3396, 3496
OliI	CACNNNNGTG	1 cut(s)	3635
PaeI	GCATGC	4 cut(s)	1127, 1506, 3067, 3487
PaeR7I	CTCGAG	1 cut(s)	233
PagI	TCATGA	1 cut(s)	2197
PciI	ACATGT	1 cut(s)	735
PcsI	WCGNNNNNNNCGW	1 cut(s)	119
PctI	GAATGC	3 cut(s)	573, 2582, 3394
PdmI	GAANNNNTTC	5 cut(s)	684, 918, 1105, 1173, 1281
PfeI	GAWTC	9 cut(s)	68, 124, 1469, 1961, 2087, 2719, 2803, 2864, 3532
PflFI	GACNNNGTC	1 cut(s)	1076
PflMI	CCANNNNNTGG	1 cut(s)	481
PleI	GAGTC	4 cut(s)	874, 2605, 2743, 3207
PpsI	GAGTC	4 cut(s)	874, 2605, 2743, 3207
Ppu21I	YACGTR	1 cut(s)	1934
PscI	ACATGT	1 cut(s)	735
Psp124BI	GAGCTC	3 cut(s)	101, 168, 3373
Psp6I	CCWGG	5 cut(s)	673, 707, 1735, 2692, 3267
PspGI	CCWGG	5 cut(s)	673, 707, 1735, 2692, 3267
PspN4I	GGNNCC	7 cut(s)	108, 454, 902, 1786, 1822, 2443, 3577
PspPI	GGNCC	6 cut(s)	1766, 1785, 2131, 2442, 2668, 3092
PspXI	VCTCGAGB	1 cut(s)	233
PsrI	GAACNNNNNNTAC	2 cut(s)	1507, 1539
PstNI	CAGNNNCTG	4 cut(s)	229, 565, 1466, 2453
PsuI	RGATCY	1 cut(s)	3620
PsyI	GACNNNGTC	1 cut(s)	1076
RruI	TCGCGA	1 cut(s)	2760
RsaI	GTAC	5 cut(s)	448, 874, 3140, 3416, 3577
RsaNI	GTAC	5 cut(s)	447, 873, 3139, 3415, 3576
SacI	GAGCTC	3 cut(s)	101, 168, 3373
SaqAI	TTAA	9 cut(s)	14, 659, 939, 950, 1367, 1563, 1833, 1914, 2571
Sau96I	GGNCC	6 cut(s)	1766, 1785, 2131, 2442, 2668, 3092
ScaI	AGTACT	2 cut(s)	874, 3140
SchI	GAGTC	4 cut(s)	875, 2606, 2744, 3207
ScrFI	CCNGG	7 cut(s)	675, 709, 1737, 2694, 3269, 3634, 3635
SduI	GDGCHC	7 cut(s)	101, 168, 457, 903, 1825, 2463, 3373
SexAI	ACCWGGT	1 cut(s)	2692
SfaNI	GCATC	8 cut(s)	30, 77, 160, 1059, 1350, 1631, 2752, 3481
SfcI	CTRYAG	1 cut(s)	750
Sfr274I	CTCGAG	1 cut(s)	233
SinI	GGWCC	3 cut(s)	2131, 2442, 2668
SlaI	CTCGAG	1 cut(s)	233
SmaI	CCCGGG	1 cut(s)	3635
SmlI	CTYRAG	8 cut(s)	233, 250, 658, 1366, 1642, 2369, 2732, 2847
SmoI	CTYRAG	8 cut(s)	233, 250, 658, 1366, 1642, 2369, 2732, 2847
SnaBI	TACGTA	1 cut(s)	1934
SphI	GCATGC	4 cut(s)	1127, 1506, 3067, 3487
SspI	AATATT	1 cut(s)	1174
SspMI	CTAG	9 cut(s)	437, 792, 972, 1632, 1673, 2297, 2342, 2795, 2814
SstI	GAGCTC	3 cut(s)	101, 168, 3373
StyD4I	CCNGG	7 cut(s)	673, 707, 1735, 2692, 3267, 3632, 3633
StyI	CCWWGG	6 cut(s)	189, 605, 1391, 2091, 2477, 3129
TaaI	ACNGT	7 cut(s)	1061, 2404, 3079, 3181, 3364, 3563, 3585
TaiI	ACGT	1 cut(s)	1936
TaqI	TCGA	8 cut(s)	47, 168, 234, 1303, 2468, 2908, 3367, 3568
TaqII	GACCGA	1 cut(s)	67
TatI	WGTACW	3 cut(s)	446, 872, 3138
TauI	GCSGC	5 cut(s)	229, 1264, 2433, 2820, 3473
TfiI	GAWTC	9 cut(s)	68, 124, 1469, 1961, 2087, 2719, 2803, 2864, 3532
Tru1I	TTAA	9 cut(s)	14, 659, 939, 950, 1367, 1563, 1833, 1914, 2571
Tru9I	TTAA	9 cut(s)	14, 659, 939, 950, 1367, 1563, 1833, 1914, 2571
TscAI	CASTG	6 cut(s)	1467, 1579, 2133, 2407, 3194, 3596
TseFI	GTSAC	3 cut(s)	1715, 2114, 3425
Tsp45I	GTSAC	3 cut(s)	1715, 2114, 3425
TspGWI	ACGGA	6 cut(s)	32, 36, 43, 498, 1874, 2702
TspMI	CCCGGG	1 cut(s)	3633
TspRI	CASTG	6 cut(s)	1467, 1579, 2133, 2407, 3194, 3596
Tth111I	GACNNNGTC	1 cut(s)	1076
Van91I	CCANNNNNTGG	1 cut(s)	481
Vha464I	CTTAAG	2 cut(s)	658, 1366
VpaK11BI	GGWCC	3 cut(s)	2131, 2442, 2668
XagI	CCTNNNNNAGG	1 cut(s)	2640
XapI	RAATTY	7 cut(s)	49, 524, 1104, 1916, 2589, 2651, 3158
XhoI	CTCGAG	1 cut(s)	233
XmaI	CCCGGG	1 cut(s)	3633
XmnI	GAANNNNTTC	5 cut(s)	684, 918, 1105, 1173, 1281
XspI	CTAG	9 cut(s)	437, 792, 972, 1632, 1673, 2297, 2342, 2795, 2814
ZrmI	AGTACT	2 cut(s)	874, 3140
Zsp2I	ATGCAT	2 cut(s)	3396, 3496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.