Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0002376

Physical Location & Seq

Genomic Coordinates Forward (+)

64422766 .. 64428819

Gene Structure 6054 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG334900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3699 bp

ATGGCGGCACCATTTCCAGGCGGTGGAGGAGGTGTCGCGGTAATGGCTGGTCCGTTAACTCCGCTCGATCCGTCTCCGTCCAAGACTTGCCTGAAGCACTCGCCGCACAAGTCTCCGATTCTCATCTTCTTGCTGTTTCACAAGGCTATTCGCTCCGAGCTCGACGGCCTCCACTGTGCCGCCATGGCCTTCGCCACCGGCCGAGCCGCCGGCGCCGGAATCGAGCCGCTGCTGGAGCGCTACCACTTTCTTCGCGCCATCTATAAGCACCACTGCAATGCGGAGGACGAGGTCATTTTTCCAGCTCTGGATATTCGTGTGAAGAATGTGGCTCGAACTTACTCCCTCGAGCACGAGGGAGAGAGTGTTATTTTTGATCAATTATTTGAGCTGCTAAACTCGAATATGCAAAATGAGGAAAGCTACCGGAGAGAGTTGGCTTCTTGTACTGGAGCTCTTCAAACATCAATTAGCCAGCATATGTTCAAGGAAGAGGAACAGGTCTTCCCATTGCTTATTGAGAAGTTTTCATGTGAAGAACAGGCATCGTTGGTCTGGCAGTTTCTGTGTAGCATTCCTGTTAACATGATGGCAGAATTTCTTCCCTGGCTCTCGTCTTCCATATCATGTGATGAACGTCAGGATATGCACAAGTACTTGAGCAAGGTAATACCAGAAGAGAAGCTTCTGCAGCAGGTTGTATTTTCCTGGATGGAAGGGGTAAAAGCTTCTGCTTGCAAAGATAAATCCAAGGGTCAATTTCAAGATTCTGGAAAGAAGGACCAGTGTGCATGTCAGTCTTCCAAGACTTGTAAAAGGAAACGCATGGAGCTAAAATCTGAACATTCGAATTTAAATCCTATAGATGAAATGTTGCTTTGGCATAATGCTATAAAAAGAGAATTGAACGACATAGCAGAGGCAGCCAGGAAGATACAACTTTCCGGTGATTTTTCTGATTTTTCGGCATTCAACAAGAGGTTGCAATTTATTGCAGAAGTTTGCATTTTTCATAGCATTGCTGAGGACAAAGTTATATTCCCTGCTCTGGATGCTGAACTCAATTTTGCTCAGGAGCATAAAGATGAAGAAATTCAGTTTGACGAGCTTAGGCGCCTCATGGAAAGCATCCAGAGGGCAGGAGCTGAATCATCTACTTCTGAATTTTATACGAATTTGTGCTCTCACGCTGATCAGATAATAGATAGCATACTGAAACACTTCCAGAGTGAGGAACTTCAGGTGCTTCCACTTGCCCGAAAGCATTTTAGCCCTAGAAGACAACGAGAACTTCTGTATCAAAGCTTATGTATGATGCCATTGAAGCTGATTGAATGTGTCTTACCATGGTTGGTAGGATCACTTAGTGATGAAGAAGCAAGTTCCTTCCTTCAGAACATTTATATAGCAGCTCCAGCATCAGATTCAGCATTGGTCACTCTTTTTTCTGGTTGGGCATGCAAAGGTCGTTCTAAGAACATTTGCTTGTCTTCGAGTGCAATTGGTTGTTGTTCTGCAACAACATTGACTGAAAGTGAAAAAGTGAGTAGCGAGAAACCATTCTGTTCGTGCACCTCTATGTTTTCCACTAAGCAAAGGCCTCTGTGCATATCAACAGATGGAGAAGATGATAATCAAAGGCCATCCAAGTGTGAAAGTTTGGCGTCATCTGTGGAAACTATAGCAGGGCAGCCTATAGACAATGGAAATACCCTTCATATATCTTGCAATAACCAGACATGCTGTGTCCCGGGGTTGGGAGTAAATGACAGTAACTTGGGAGTGGGTTCTCTAGCTGCAGTAAAATCTCTTCGTGCCTTAACTTTTAATCCTTCAGCTCCTTCACTCAACTCTAGTCTTTTCAATTGGGAAACAGATTTCAGCTCTGAAGATACTAGTACTGGAACACGACCTATTGATAACATATTTAAATTCCATAAAGCTATACGTAAAGACTTGGAGTATTTAGATATTGAATCAGGAAAGCTTAATGATTGTAATGAGACTTTTATCAGGCATTTTTCTGGTAGGTTTCGTTTGTTATGGGGTCTATATAGAGCTCATAGTAATGCAGAGGACGATATTGTGTTCCCAGCATTGGAATCGAAAGAGACTCTTCATAATGTTAGCCATTCTTACACACTGGATCACAAGCAGGAAGAGAAACTGTTTGAGGATATTTTTTCAGTTCTTTCTGAGCTTGCACAACTTAGCGAGTTCATGAGCATTAGAAATATGTCTGCTGATTCAGGACGAAGTAATTATGATTCTTTCGAGCATAGTGATACTTTGAGAAAGTACAATGAGCTAGCTACAAAGCTTCAGGGGATGTGCAAATCCATTAGAGTGACATTGGATCAACATGTTTTCCGTGAGGAACTCGAGTTGTGGCCATTGTTTGATAAACATTTTTCTGTGGAAGAACAAGATAAAATTGTTGGCAGGATAATTGGTACTACAGGTGCAGAGGTTCTCCAATCAATGCTGCCATGGGTAACTGCTGCTCTTACTCAGGAAGAACAGAATAAATTGATGGACACATGGAAGCAGGCGACCAAAAATACCATGTTCAGTGAATGGCTTGACGAATGGTGGGATGGAACTCGTGTTGAATCTTCACATACAGAGACACCAGAAACTTGTACTTCTGTAGTTTCAGATGCGGATGCATATGCTAGCTTGGAACAGAGTGATGAAACATTTAAACCTGGATGGAAAGATATATTTCGGATGAATCAGAATGAGCTTGAATCAGAAATAAGAAAGGTTGCTCGAGATTCAACTCTTGATCCAAGAAGAAAAGCATATCTTATTCAAAATCTAATGACTAGTCGCTGGATAGCTTCTCAGCAGAAATCACCTCAAGCAGGTGCTGTTGAAGGTTCAAATGGTGAGGATTTGCTTGGATGTGCACCGTCATTCCATGATTCAGAGAAAGAATTGTTTGGCTGTAAGCATTACAAAAGAAATTGTAAAGTCCGCGCCAGTTGCTGTGGAAAACTATTTACATGCAGGTTCTGCCATGATGAAGTGAGCGACCACTCAATGGATAGGAAGGCTACATCTGAAATGATGTGTATGCGCTGCTTGAAGATTCAGCCTGTGGGACCAGTCTGCACAACATCTTCCTGTGGTGGATTCTTGATGGCAAAGTACTACTGTAACATTTGCAAATTTTTTGATGATGAAAGGACGGTTTATCATTGTCCTTCCTGCAATTTATGCCGTGTTGGGAAGGGACTTGGTGTCGACTTCTTTCATTGCATGACATGCAATTGTTGCCTGGGGATAAAGTTACTAGACCACAAATGTAGGGAGAAGGGCCTAGAAATAAACTGTCCCATCTGCTGCGACTTTTTGTTTACATCAAGTGCAACTGTGAGATCCCTTCCTTGTGGCCATTACATGCATTCAGCTTGCTTCCAGGCATACACTTGTAGTCACTACATTTGCCCAATCTGCAGCAAATCTTTGGGAGATATGGCGGTTTACTTTGGCATGCTTGATGCACTACTGGCTTCTGAGGAACTCCCAGAAGAATACAGGGATCGCTGTCAGGACATTCTCTGCAATGACTGTGATAAGAAGGGGACAGCGCCGTTCCACTGGCTGTACCATAAGTGTGGCTCTTGTGGATCCTATAACACGAAGGTAATCAGGATTGATTCGAGCAACCACTGCTTAACATCAGGCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hemerythrin zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

1232

Amino Acids

138.76

Weight (kDa)

5.66

Isoelectric Point (pI)

55.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hemerythrin	PF01814	43 - 172	3.9e-10	Hemerythrin HHE cation binding domain
Hemerythrin	PF01814	287 - 419	5.7e-08	Hemerythrin HHE cation binding domain
zf-CHY	PF05495	984 - 1052	6.6e-19	CHY zinc finger
zf-RING_2	PF13639	1112 - 1154	2.8e-06	Ring finger domain
zinc_ribbon_6	PF14599	1160 - 1217	8.3e-24	Zinc-ribbon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18290
fragaria_vesca	FvH4_4g26990 FvH4_4g26990 FvH4_4g26990 FvH4_4g26990 FvH4_5g11860 FvH4_5g11860 FvH4_5g11860
malus_domestica	MD06G1217900.v1.1 MD14G1228100.v1.1 MD16G1039900.v1.1
prunus_persica	Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1
pyrus_communis	pycom06g19410 pycom14g18950 pycom16g03460
rosa_chinensis	RchiOBHm_Chr4g0434791 RchiOBHm_Chr7g0185731
rosa_laevigata	RLG00000004888 RLG00000006649
rosa_multiflora	Rmu_sc0000179.1_g000001 Rmu_sc0002952.1_g000002
rosa_roxburghii	Rroxscaffold_3G00268670 Rroxscaffold_5G00376000
rosa_rugosa	Rorug04G0278300 Rorug04G0278400 Rorug06G0470900
rosa_samantha	Rh4AG334900 Rh4CG357800 Rh4DG337300 Rh7AG071600 Rh7BG076200 Rh7DG076200
rosa_wichuraiana	Rw4G029200 Rw7G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2858
Acc36I	ACCTGC	3 cut(s)	685, 2858, 3003
AccB1I	GGYRCC	3 cut(s)	7, 212, 1113
AccB7I	CCANNNNNTGG	2 cut(s)	23, 3046
AccBSI	CCGCTC	1 cut(s)	64
AccI	GTMKAC	1 cut(s)	3249
AccII	CGCG	3 cut(s)	38, 255, 2982
AclWI	GGATC	9 cut(s)	62, 1366, 2154, 2364, 2783, 3378, 3555, 3630, 3643
AcoI	YGGCCR	3 cut(s)	199, 2390, 3397
AcsI	RAATTY	7 cut(s)	596, 850, 1092, 1163, 1174, 1931, 3173
AcuI	CTGAAG	6 cut(s)	113, 1223, 1376, 1818, 1908, 2306
AcyI	GRCGYC	3 cut(s)	213, 1114, 1664
AdeI	CACNNNGTG	1 cut(s)	1367
AfaI	GTAC	8 cut(s)	448, 656, 1900, 2300, 2455, 2644, 3155, 3614
AfeI	AGCGCT	1 cut(s)	239
AfiI	CCNNNNNNNGG	9 cut(s)	17, 23, 1048, 1231, 1756, 1757, 2098, 2867, 3046
AflIII	ACRYGT	1 cut(s)	2362
AhdI	GACNNNNNGTC	1 cut(s)	3245
AhlI	ACTAGT	2 cut(s)	1895, 2828
AjnI	CCWGG	7 cut(s)	16, 605, 707, 926, 2707, 3282, 3423
AloI	GAACNNNNNNTCC	2 cut(s)	489, 521
Alw21I	GWGCWC	7 cut(s)	162, 354, 457, 1184, 1574, 2062, 2914
Alw26I	GTCTC	5 cut(s)	78, 117, 1997, 2105, 2621
Alw44I	GTGCAC	2 cut(s)	1570, 2910
AlwI	GGATC	9 cut(s)	62, 1366, 2154, 2364, 2783, 3378, 3555, 3630, 3643
AlwNI	CAGNNNCTG	5 cut(s)	565, 1145, 2873, 2991, 3018
Ama87I	CYCGRG	4 cut(s)	347, 1750, 2381, 2772
Aor51HI	AGCGCT	1 cut(s)	239
AoxI	GGCC	8 cut(s)	166, 186, 199, 1598, 1640, 2390, 3322, 3397
ApaLI	GTGCAC	2 cut(s)	1570, 2910
ApoI	RAATTY	7 cut(s)	596, 850, 1092, 1163, 1174, 1931, 3173
Asp700I	GAANNNNTTC	4 cut(s)	600, 1092, 1220, 1559
AspLEI	GCGC	7 cut(s)	215, 240, 257, 1116, 2984, 3084, 3598
AspS9I	GGNCC	4 cut(s)	50, 781, 3107, 3322
AsuC2I	CCSGG	2 cut(s)	1751, 1752
AsuHPI	GGTGA	3 cut(s)	959, 2850, 2903
AsuII	TTCGAA	1 cut(s)	848
AsuNHI	GCTAGC	2 cut(s)	2308, 2675
AvaI	CYCGRG	4 cut(s)	347, 1750, 2381, 2772
AvaII	GGWCC	3 cut(s)	50, 781, 3107
BaeGI	GKGCMC	2 cut(s)	1574, 2914
BaeI	ACNNNNGTAYC	2 cut(s)	1280, 1313
BalI	TGGCCA	2 cut(s)	2392, 3399
BamHI	GGATCC	1 cut(s)	3635
BanI	GGYRCC	3 cut(s)	7, 212, 1113
BanII	GRGCYC	3 cut(s)	162, 457, 2062
BarI	GAAGNNNNNNTAC	2 cut(s)	1794, 1826
BauI	CACGAG	2 cut(s)	353, 2604
BbsI	GAAGAC	5 cut(s)	496, 609, 792, 1285, 1482
Bbv12I	GWGCWC	7 cut(s)	162, 354, 457, 1184, 1574, 2062, 2914
BbvCI	CCTCAGC	1 cut(s)	1023
BceAI	ACGGC	3 cut(s)	181, 3210, 3583
BcgI	CGANNNNNNTGC	2 cut(s)	2085, 2119
BciT130I	CCWGG	7 cut(s)	18, 607, 709, 928, 2709, 3284, 3425
BclI	TGATCA	2 cut(s)	376, 1192
BcnI	CCSGG	2 cut(s)	1751, 1752
BcoDI	GTCTC	5 cut(s)	78, 117, 1997, 2105, 2621
BcuI	ACTAGT	2 cut(s)	1895, 2828
BfaI	CTAG	9 cut(s)	1275, 1793, 1854, 1896, 2309, 2676, 2829, 3299, 3326
BfmI	CTRYAG	8 cut(s)	689, 861, 1680, 1695, 1797, 2457, 2649, 3460
BfoI	RGCGCY	4 cut(s)	216, 241, 1117, 3599
BfuAI	ACCTGC	3 cut(s)	685, 2858, 3003
BglI	GCCNNNNNGGC	1 cut(s)	185
BmcAI	AGTACT	3 cut(s)	656, 1900, 3155
Bme1390I	CCNGG	9 cut(s)	18, 607, 709, 928, 1751, 1752, 2709, 3284, 3425
Bme18I	GGWCC	3 cut(s)	50, 781, 3107
BmeRI	GACNNNNNGTC	1 cut(s)	3245
BmeT110I	CYCGRG	4 cut(s)	347, 1750, 2381, 2772
BmgT120I	GGNCC	4 cut(s)	50, 781, 3107, 3322
BmiI	GGNNCC	5 cut(s)	9, 214, 1115, 3108, 3637
BmrFI	CCNGG	9 cut(s)	18, 607, 709, 928, 1751, 1752, 2709, 3284, 3425
BmsI	GCATC	8 cut(s)	554, 1042, 1137, 1305, 1427, 2650, 2656, 3496
BmtI	GCTAGC	2 cut(s)	2312, 2679
BpiI	GAAGAC	5 cut(s)	496, 609, 792, 1285, 1482
BpmI	CTGGAG	3 cut(s)	254, 471, 1398
Bpu10I	CCTNAGC	3 cut(s)	1023, 1071, 1109
Bpu14I	TTCGAA	1 cut(s)	848
BpuEI	CTTGAG	2 cut(s)	679, 2847
BpuMI	CCSGG	2 cut(s)	1751, 1752
BsaAI	YACGTR	1 cut(s)	1949
BsaBI	GATNNNNATC	2 cut(s)	122, 1632
BsaHI	GRCGYC	3 cut(s)	213, 1114, 1664
BsaJI	CCNNGG	8 cut(s)	183, 605, 750, 1346, 1750, 1751, 2489, 3283
BsaWI	WCCGGW	2 cut(s)	426, 944
BsaXI	ACNNNNNCTCC	4 cut(s)	18, 48, 351, 381
Bsc4I	CCNNNNNNNGG	9 cut(s)	17, 23, 1048, 1231, 1756, 1757, 2098, 2867, 3046
Bse118I	RCCGGY	2 cut(s)	197, 209
Bse1I	ACTGG	8 cut(s)	454, 784, 1906, 2148, 2985, 3110, 3519, 3611
Bse3DI	GCAATG	5 cut(s)	283, 509, 1017, 3259, 3577
Bse8I	GATNNNNATC	2 cut(s)	122, 1632
BseBI	CCWGG	7 cut(s)	18, 607, 709, 928, 2709, 3284, 3425
BseDI	CCNNGG	8 cut(s)	183, 605, 750, 1346, 1750, 1751, 2489, 3283
BseJI	GATNNNNATC	2 cut(s)	122, 1632
BseLI	CCNNNNNNNGG	9 cut(s)	17, 23, 1048, 1231, 1756, 1757, 2098, 2867, 3046
BseMI	GCAATG	5 cut(s)	283, 509, 1017, 3259, 3577
BseMII	CTCAG	6 cut(s)	1014, 1085, 2187, 2525, 2861, 3513
BseNI	ACTGG	8 cut(s)	454, 784, 1906, 2148, 2985, 3110, 3519, 3611
BseRI	GAGGAG	1 cut(s)	42
BseSI	GKGCMC	2 cut(s)	1574, 2914
BseX3I	CGGCCG	1 cut(s)	199
BseYI	CCCAGC	1 cut(s)	2092
BsgI	GTGCAG	2 cut(s)	2484, 3100
Bsh1236I	CGCG	3 cut(s)	38, 255, 2982
Bsh1285I	CGRYCG	1 cut(s)	202
BshFI	GGCC	8 cut(s)	168, 188, 201, 1600, 1642, 2392, 3324, 3399
BshNI	GGYRCC	3 cut(s)	7, 212, 1113
BsiEI	CGRYCG	1 cut(s)	202
BsiHKAI	GWGCWC	7 cut(s)	162, 354, 457, 1184, 1574, 2062, 2914
BsiHKCI	CYCGRG	4 cut(s)	347, 1750, 2381, 2772
BsiSI	CCGG	6 cut(s)	198, 210, 216, 427, 945, 1751
BslFI	GGGAC	5 cut(s)	1733, 3120, 3252, 3324, 3604
BslI	CCNNNNNNNGG	9 cut(s)	17, 23, 1048, 1231, 1756, 1757, 2098, 2867, 3046
BsmAI	GTCTC	5 cut(s)	78, 117, 1997, 2105, 2621
BsmBI	CGTCTC	1 cut(s)	78
BsmFI	GGGAC	5 cut(s)	1733, 3120, 3252, 3324, 3604
BsmI	GAATGC	3 cut(s)	573, 968, 3409
BsnI	GGCC	8 cut(s)	168, 188, 201, 1600, 1642, 2392, 3324, 3399
BsoBI	CYCGRG	4 cut(s)	347, 1750, 2381, 2772
Bsp119I	TTCGAA	1 cut(s)	848
Bsp1286I	GDGCHC	7 cut(s)	162, 354, 457, 1184, 1574, 2062, 2914
Bsp19I	CCATGG	3 cut(s)	183, 1346, 2489
BspANI	GGCC	8 cut(s)	168, 188, 201, 1600, 1642, 2392, 3324, 3399
BspCNI	CTCAG	6 cut(s)	1015, 1084, 2188, 2524, 2860, 3514
BspFNI	CGCG	3 cut(s)	38, 255, 2982
BspHI	TCATGA	1 cut(s)	2220
BspLI	GGNNCC	5 cut(s)	9, 214, 1115, 3108, 3637
BspMAI	CTGCAG	3 cut(s)	693, 1801, 3464
BspMI	ACCTGC	3 cut(s)	685, 2858, 3003
BspOI	GCTAGC	2 cut(s)	2312, 2679
BspPI	GGATC	9 cut(s)	62, 1366, 2154, 2364, 2783, 3378, 3555, 3630, 3643
BspQI	GCTCTTC	1 cut(s)	462
BspT104I	TTCGAA	1 cut(s)	848
BspT107I	GGYRCC	3 cut(s)	7, 212, 1113
BsrBI	CCGCTC	1 cut(s)	64
BsrDI	GCAATG	5 cut(s)	283, 509, 1017, 3259, 3577
BsrFI	RCCGGY	2 cut(s)	197, 209
BsrI	ACTGG	8 cut(s)	454, 784, 1906, 2148, 2985, 3110, 3519, 3611
BssAI	RCCGGY	2 cut(s)	197, 209
BssECI	CCNNGG	8 cut(s)	183, 605, 750, 1346, 1750, 1751, 2489, 3283
BssNI	GRCGYC	3 cut(s)	213, 1114, 1664
BssSI	CACGAG	2 cut(s)	353, 2604
BssT1I	CCWWGG	4 cut(s)	183, 750, 1346, 2489
Bst2BI	CACGAG	2 cut(s)	353, 2604
Bst2UI	CCWGG	7 cut(s)	18, 607, 709, 928, 2709, 3284, 3425
Bst4CI	ACNGT	9 cut(s)	176, 1772, 2169, 2916, 3161, 3196, 3338, 3379, 3578
Bst6I	CTCTTC	6 cut(s)	462, 486, 672, 1815, 2121, 2154
BstACI	GRCGYC	3 cut(s)	213, 1114, 1664
BstAPI	GCANNNNNTGC	5 cut(s)	3018, 3222, 3270, 3279, 3678
BstBAI	YACGTR	1 cut(s)	1949
BstBI	TTCGAA	1 cut(s)	848
BstDSI	CCRYGG	3 cut(s)	183, 1346, 2489
BstENI	CCTNNNNNAGG	1 cut(s)	2865
BstFNI	CGCG	3 cut(s)	38, 255, 2982
BstH2I	RGCGCY	4 cut(s)	216, 241, 1117, 3599
BstHHI	GCGC	7 cut(s)	215, 240, 257, 1116, 2984, 3084, 3598
BstMAI	GTCTC	5 cut(s)	78, 117, 1997, 2105, 2621
BstMCI	CGRYCG	1 cut(s)	202
BstNI	CCWGG	7 cut(s)	18, 607, 709, 928, 2709, 3284, 3425
BstNSI	RCATGY	8 cut(s)	795, 1461, 1743, 2366, 3012, 3273, 3409, 3502
BstSCI	CCNGG	9 cut(s)	16, 605, 707, 926, 1749, 1750, 2707, 3282, 3423
BstSFI	CTRYAG	8 cut(s)	689, 861, 1680, 1695, 1797, 2457, 2649, 3460
BstSLI	GKGCMC	2 cut(s)	1574, 2914
BstSNI	TACGTA	1 cut(s)	1949
BstUI	CGCG	3 cut(s)	38, 255, 2982
BstV2I	GAAGAC	5 cut(s)	496, 609, 792, 1285, 1482
BstX2I	RGATCY	2 cut(s)	3383, 3635
BstXI	CCANNNNNNTGG	1 cut(s)	3623
BstYI	RGATCY	2 cut(s)	3383, 3635
BstZI	CGGCCG	1 cut(s)	199
BsuRI	GGCC	8 cut(s)	168, 188, 201, 1600, 1642, 2392, 3324, 3399
BtgI	CCRYGG	3 cut(s)	183, 1346, 2489
BtsI	GCAGTG	2 cut(s)	271, 3676
BtsIMutI	CAGTG	7 cut(s)	172, 271, 791, 2141, 2578, 3604, 3676
BveI	ACCTGC	3 cut(s)	685, 2858, 3003
CaiI	CAGNNNCTG	5 cut(s)	565, 1145, 2873, 2991, 3018
CciI	TCATGA	1 cut(s)	2220
CfoI	GCGC	7 cut(s)	215, 240, 257, 1116, 2984, 3084, 3598
Cfr10I	RCCGGY	2 cut(s)	197, 209
Cfr13I	GGNCC	4 cut(s)	50, 781, 3107, 3322
Cfr9I	CCCGGG	1 cut(s)	1750
CseI	GACGC	1 cut(s)	1653
Csp6I	GTAC	8 cut(s)	447, 655, 1899, 2299, 2454, 2643, 3154, 3613
CviQI	GTAC	8 cut(s)	447, 655, 1899, 2299, 2454, 2643, 3154, 3613
DinI	GGCGCC	2 cut(s)	214, 1115
DraI	TTTAAA	3 cut(s)	855, 1930, 2704
DraIII	CACNNNGTG	1 cut(s)	1367
DriI	GACNNNNNGTC	1 cut(s)	3245
EaeI	YGGCCR	3 cut(s)	199, 2390, 3397
EagI	CGGCCG	1 cut(s)	199
Eam1104I	CTCTTC	6 cut(s)	462, 486, 672, 1815, 2121, 2154
Eam1105I	GACNNNNNGTC	1 cut(s)	3245
EarI	CTCTTC	6 cut(s)	462, 486, 672, 1815, 2121, 2154
Ecl136II	GAGCTC	3 cut(s)	160, 455, 2060
EclXI	CGGCCG	1 cut(s)	199
Eco105I	TACGTA	1 cut(s)	1949
Eco130I	CCWWGG	4 cut(s)	183, 750, 1346, 2489
Eco147I	AGGCCT	1 cut(s)	1600
Eco24I	GRGCYC	3 cut(s)	162, 457, 2062
Eco47I	GGWCC	3 cut(s)	50, 781, 3107
Eco47III	AGCGCT	1 cut(s)	239
Eco52I	CGGCCG	1 cut(s)	199
Eco53kI	GAGCTC	3 cut(s)	160, 455, 2060
Eco57I	CTGAAG	6 cut(s)	113, 1223, 1376, 1818, 1908, 2306
Eco88I	CYCGRG	4 cut(s)	347, 1750, 2381, 2772
EcoICRI	GAGCTC	3 cut(s)	160, 455, 2060
EcoNI	CCTNNNNNAGG	1 cut(s)	2865
EcoO109I	RGGNCCY	1 cut(s)	3322
EcoRII	CCWGG	7 cut(s)	16, 605, 707, 926, 2707, 3282, 3423
EcoT14I	CCWWGG	4 cut(s)	183, 750, 1346, 2489
EcoT22I	ATGCAT	2 cut(s)	2671, 3411
EcoT38I	GRGCYC	3 cut(s)	162, 457, 2062
EgeI	GGCGCC	2 cut(s)	214, 1115
EheI	GGCGCC	2 cut(s)	214, 1115
ErhI	CCWWGG	4 cut(s)	183, 750, 1346, 2489
Esp3I	CGTCTC	1 cut(s)	78
FalI	AAGNNNNNCTT	6 cut(s)	669, 701, 2100, 2132, 2793, 2825
FaqI	GGGAC	5 cut(s)	1733, 3120, 3252, 3324, 3604
FauNDI	CATATG	2 cut(s)	480, 2671
FbaI	TGATCA	2 cut(s)	376, 1192
FblI	GTMKAC	1 cut(s)	3249
FriOI	GRGCYC	3 cut(s)	162, 457, 2062
FspBI	CTAG	9 cut(s)	1275, 1793, 1854, 1896, 2309, 2676, 2829, 3299, 3326
GlaI	GCGC	7 cut(s)	214, 239, 256, 1115, 2983, 3083, 3597
GsaI	CCCAGC	1 cut(s)	2096
GsuI	CTGGAG	3 cut(s)	254, 471, 1398
HaeII	RGCGCY	4 cut(s)	216, 241, 1117, 3599
HaeIII	GGCC	8 cut(s)	168, 188, 201, 1600, 1642, 2392, 3324, 3399
HapII	CCGG	6 cut(s)	198, 210, 216, 427, 945, 1751
HgaI	GACGC	1 cut(s)	1653
HhaI	GCGC	7 cut(s)	215, 240, 257, 1116, 2984, 3084, 3598
Hin1I	GRCGYC	3 cut(s)	213, 1114, 1664
Hin6I	GCGC	7 cut(s)	213, 238, 255, 1114, 2982, 3082, 3596
HinP1I	GCGC	7 cut(s)	213, 238, 255, 1114, 2982, 3082, 3596
HincII	GTYRAC	3 cut(s)	57, 583, 3250
HindII	GTYRAC	3 cut(s)	57, 583, 3250
HindIII	AAGCTT	5 cut(s)	683, 726, 1303, 1985, 2318
HpaI	GTTAAC	2 cut(s)	57, 583
HpaII	CCGG	6 cut(s)	198, 210, 216, 427, 945, 1751
HphI	GGTGA	3 cut(s)	959, 2850, 2903
Hpy166II	GTNNAC	7 cut(s)	57, 583, 1572, 2912, 3250, 3363, 3490
Hpy8I	GTNNAC	7 cut(s)	57, 583, 1572, 2912, 3250, 3363, 3490
Hpy99I	CGWCG	1 cut(s)	167
HpyCH4III	ACNGT	9 cut(s)	176, 1772, 2169, 2916, 3161, 3196, 3338, 3379, 3578
HpyCH4IV	ACGT	2 cut(s)	637, 1948
HpySE526I	ACGT	2 cut(s)	637, 1948
Hsp92I	GRCGYC	3 cut(s)	213, 1114, 1664
HspAI	GCGC	7 cut(s)	213, 238, 255, 1114, 2982, 3082, 3596
KasI	GGCGCC	2 cut(s)	212, 1113
KroI	GCCGGC	1 cut(s)	209
KroNI	GCCGGC	1 cut(s)	211
Ksp22I	TGATCA	2 cut(s)	376, 1192
KspAI	GTTAAC	2 cut(s)	57, 583
LguI	GCTCTTC	1 cut(s)	462
LmnI	GCTCC	8 cut(s)	158, 235, 452, 829, 1075, 1142, 1417, 1843
LweI	GCATC	8 cut(s)	554, 1042, 1137, 1305, 1427, 2650, 2656, 3496
MaeI	CTAG	9 cut(s)	1275, 1793, 1854, 1896, 2309, 2676, 2829, 3299, 3326
MaeII	ACGT	2 cut(s)	637, 1948
MaeIII	GTNAC	7 cut(s)	1435, 1772, 2347, 2494, 3161, 3294, 3440
MbiI	CCGCTC	1 cut(s)	64
MfeI	CAATTG	3 cut(s)	1500, 1864, 3274
MflI	RGATCY	2 cut(s)	3383, 3635
MhlI	GDGCHC	7 cut(s)	162, 354, 457, 1184, 1574, 2062, 2914
MlsI	TGGCCA	2 cut(s)	2392, 3399
MluNI	TGGCCA	2 cut(s)	2392, 3399
Mly113I	GGCGCC	2 cut(s)	213, 1114
MlyI	GAGTC	1 cut(s)	2107
Mox20I	TGGCCA	2 cut(s)	2392, 3399
Mph1103I	ATGCAT	2 cut(s)	2671, 3411
MreI	CGCCGGCG	1 cut(s)	209
MroNI	GCCGGC	1 cut(s)	209
MroXI	GAANNNNTTC	4 cut(s)	600, 1092, 1220, 1559
MscI	TGGCCA	2 cut(s)	2392, 3399
MseI	TTAA	9 cut(s)	56, 582, 854, 1820, 1827, 1929, 1989, 2703, 3683
MslI	CAYNNNNRTG	7 cut(s)	276, 1083, 1577, 1648, 2067, 2345, 3621
Msp20I	TGGCCA	2 cut(s)	2392, 3399
MspA1I	CMGCKG	1 cut(s)	229
MspI	CCGG	6 cut(s)	198, 210, 216, 427, 945, 1751
MspR9I	CCNGG	9 cut(s)	18, 607, 709, 928, 1751, 1752, 2709, 3284, 3425
MunI	CAATTG	3 cut(s)	1500, 1864, 3274
Mva1269I	GAATGC	3 cut(s)	573, 968, 3409
MvaI	CCWGG	7 cut(s)	18, 607, 709, 928, 2709, 3284, 3425
MvnI	CGCG	3 cut(s)	38, 255, 2982
NaeI	GCCGGC	1 cut(s)	211
NarI	GGCGCC	2 cut(s)	213, 1114
NciI	CCSGG	2 cut(s)	1751, 1752
NcoI	CCATGG	3 cut(s)	183, 1346, 2489
NdeI	CATATG	2 cut(s)	480, 2671
NgoMIV	GCCGGC	1 cut(s)	209
NheI	GCTAGC	2 cut(s)	2308, 2675
NlaIV	GGNNCC	5 cut(s)	9, 214, 1115, 3108, 3637
NmeAIII	GCCGAG	1 cut(s)	227
NmuCI	GTSAC	3 cut(s)	1435, 2347, 3440
NsiI	ATGCAT	2 cut(s)	2671, 3411
NspI	RCATGY	8 cut(s)	795, 1461, 1743, 2366, 3012, 3273, 3409, 3502
NspV	TTCGAA	1 cut(s)	848
PaeI	GCATGC	2 cut(s)	1461, 3502
PaeR7I	CTCGAG	3 cut(s)	347, 2381, 2772
PagI	TCATGA	1 cut(s)	2220
PaqCI	CACCTGC	1 cut(s)	2858
PceI	AGGCCT	1 cut(s)	1600
PciI	ACATGT	1 cut(s)	2362
PciSI	GCTCTTC	1 cut(s)	462
PctI	GAATGC	3 cut(s)	573, 968, 3409
PdiI	GCCGGC	1 cut(s)	211
PdmI	GAANNNNTTC	4 cut(s)	600, 1092, 1220, 1559
PflFI	GACNNNGTC	1 cut(s)	290
PflMI	CCANNNNNTGG	2 cut(s)	23, 3046
PfoI	TCCNGGA	1 cut(s)	707
PleI	GAGTC	1 cut(s)	2107
PluTI	GGCGCC	2 cut(s)	216, 1117
PpsI	GAGTC	1 cut(s)	2107
Ppu21I	YACGTR	1 cut(s)	1949
PscI	ACATGT	1 cut(s)	2362
Psp124BI	GAGCTC	3 cut(s)	162, 457, 2062
Psp6I	CCWGG	7 cut(s)	16, 605, 707, 926, 2707, 3282, 3423
PspFI	CCCAGC	1 cut(s)	2092
PspGI	CCWGG	7 cut(s)	16, 605, 707, 926, 2707, 3282, 3423
PspN4I	GGNNCC	5 cut(s)	9, 214, 1115, 3108, 3637
PspPI	GGNCC	4 cut(s)	50, 781, 3107, 3322
PspXI	VCTCGAGB	2 cut(s)	347, 2381
PstI	CTGCAG	3 cut(s)	693, 1801, 3464
PstNI	CAGNNNCTG	5 cut(s)	565, 1145, 2873, 2991, 3018
PsuI	RGATCY	2 cut(s)	3383, 3635
PsyI	GACNNNGTC	1 cut(s)	290
RsaI	GTAC	8 cut(s)	448, 656, 1900, 2300, 2455, 2644, 3155, 3614
RsaNI	GTAC	8 cut(s)	447, 655, 1899, 2299, 2454, 2643, 3154, 3613
RseI	CAYNNNNRTG	7 cut(s)	276, 1083, 1577, 1648, 2067, 2345, 3621
SacI	GAGCTC	3 cut(s)	162, 457, 2062
SalI	GTCGAC	1 cut(s)	3248
SapI	GCTCTTC	1 cut(s)	462
SaqAI	TTAA	9 cut(s)	56, 582, 854, 1820, 1827, 1929, 1989, 2703, 3683
Sau96I	GGNCC	4 cut(s)	50, 781, 3107, 3322
ScaI	AGTACT	3 cut(s)	656, 1900, 3155
SchI	GAGTC	1 cut(s)	2107
ScrFI	CCNGG	9 cut(s)	18, 607, 709, 928, 1751, 1752, 2709, 3284, 3425
SduI	GDGCHC	7 cut(s)	162, 354, 457, 1184, 1574, 2062, 2914
SfaNI	GCATC	8 cut(s)	554, 1042, 1137, 1305, 1427, 2650, 2656, 3496
SfcI	CTRYAG	8 cut(s)	689, 861, 1680, 1695, 1797, 2457, 2649, 3460
SfoI	GGCGCC	2 cut(s)	214, 1115
Sfr274I	CTCGAG	3 cut(s)	347, 2381, 2772
SfuI	TTCGAA	1 cut(s)	848
SgrAI	CRCCGGYG	1 cut(s)	209
SinI	GGWCC	3 cut(s)	50, 781, 3107
SlaI	CTCGAG	3 cut(s)	347, 2381, 2772
SmaI	CCCGGG	1 cut(s)	1752
SmiI	ATTTAAAT	2 cut(s)	855, 1930
SmiMI	CAYNNNNRTG	7 cut(s)	276, 1083, 1577, 1648, 2067, 2345, 3621
SmlI	CTYRAG	5 cut(s)	347, 658, 2381, 2772, 2862
SmoI	CTYRAG	5 cut(s)	347, 658, 2381, 2772, 2862
SnaBI	TACGTA	1 cut(s)	1949
SpeI	ACTAGT	2 cut(s)	1895, 2828
SphI	GCATGC	2 cut(s)	1461, 3502
SseBI	AGGCCT	1 cut(s)	1600
SspDI	GGCGCC	2 cut(s)	212, 1113
SspMI	CTAG	9 cut(s)	1275, 1793, 1854, 1896, 2309, 2676, 2829, 3299, 3326
SstI	GAGCTC	3 cut(s)	162, 457, 2062
StuI	AGGCCT	1 cut(s)	1600
StyD4I	CCNGG	9 cut(s)	16, 605, 707, 926, 1749, 1750, 2707, 3282, 3423
StyI	CCWWGG	4 cut(s)	183, 750, 1346, 2489
SwaI	ATTTAAAT	2 cut(s)	855, 1930
TaaI	ACNGT	9 cut(s)	176, 1772, 2169, 2916, 3161, 3196, 3338, 3379, 3578
TaiI	ACGT	2 cut(s)	640, 1951
TatI	WGTACW	6 cut(s)	446, 654, 1898, 2298, 2642, 3153
TauI	GCSGC	5 cut(s)	8, 106, 182, 209, 229
Tru1I	TTAA	9 cut(s)	56, 582, 854, 1820, 1827, 1929, 1989, 2703, 3683
Tru9I	TTAA	9 cut(s)	56, 582, 854, 1820, 1827, 1929, 1989, 2703, 3683
TscAI	CASTG	7 cut(s)	179, 278, 791, 2148, 2578, 3611, 3683
TseFI	GTSAC	3 cut(s)	1435, 2347, 3440
Tsp45I	GTSAC	3 cut(s)	1435, 2347, 3440
TspGWI	ACGGA	4 cut(s)	42, 60, 66, 2360
TspMI	CCCGGG	1 cut(s)	1750
TspRI	CASTG	7 cut(s)	179, 278, 791, 2148, 2578, 3611, 3683
Tth111I	GACNNNGTC	1 cut(s)	290
Van91I	CCANNNNNTGG	2 cut(s)	23, 3046
VneI	GTGCAC	2 cut(s)	1570, 2910
VpaK11BI	GGWCC	3 cut(s)	50, 781, 3107
XagI	CCTNNNNNAGG	1 cut(s)	2865
XapI	RAATTY	7 cut(s)	596, 850, 1092, 1163, 1174, 1931, 3173
XceI	RCATGY	8 cut(s)	795, 1461, 1743, 2366, 3012, 3273, 3409, 3502
XhoI	CTCGAG	3 cut(s)	347, 2381, 2772
XmaI	CCCGGG	1 cut(s)	1750
XmiI	GTMKAC	1 cut(s)	3249
XmnI	GAANNNNTTC	4 cut(s)	600, 1092, 1220, 1559
XspI	CTAG	9 cut(s)	1275, 1793, 1854, 1896, 2309, 2676, 2829, 3299, 3326
ZrmI	AGTACT	3 cut(s)	656, 1900, 3155
Zsp2I	ATGCAT	2 cut(s)	2671, 3411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.