Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002376

Physical Location & Seq

Genomic Coordinates Forward (+)

2826995 .. 2833889

Gene Structure 6895 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1039900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3690 bp

ATGGCGGCACCATTTCCTGGCGGTGGAGGTGTGGCGGTAATGGCTGGACCCTTAGCCCCGCTCGATCCTTCTCCGTCCAAGACTTGCCTGAAGAGCTCGGCTCACAAGTCGCCGATCCTCATCTTCTTGCTGTTTCACAAGGCTATTCTCTCCGAGCTCGACGGCCTCCACCGAGCCGCCATGGCCTTCGCCACCAGCGAAGGCTCCGCCGCTGGAATTCAGCCCTTGCTGGAGGGCTACCGCTTCCTCCGCGCTATTTATAAGCACCATTGCAAGGCCGAGGACGAGGTCATATTTCCAGCTCTAGATATACGGGTGAAGAATGTGGCTCGAACGTATTCTCTTGAACACGAGGGAGAGAGCGTTCTTTTCGATCAACTGTTTGAGCTGCTAGACTCCGATAAACAAAATGAGGAGAGCTACCGGAGAGAGTTGGCTTCTTGTACTGGAGCCATCCAAACATCAATCAGCCAGCATATGTCCAAGGAAGAGGAACAGGTCTTCCCTTTGCTTATTGAGAAGTTTTCATTTGAGGAGCAAGCTTCCTTGGTGTGGCAGTTTCTGTGTAGTATTCCTGTTAACATGATGGCAGAATTTCTTCCGTGGCTCTCTTCCTCCATATCATCCGACGAACATCAGGATATGCGCAAGTACTTGAGCAAAGTAATACCAGAAGAGAAGCTTCTGCAGCAGGTTGTATTTGCCTGGATGGAAGGGGAAAATGTTTCACAGGGCAAAGGTAATTCCGAGGGGCAATTTCAAGATTCTGTAAATAAGGGGAAGTGCGCGTGCGAATCTTCCAGGACTTGTAAAAGGAAACGTGTGGACGTACAATCTGATAATTCCAGCACCATGGTTTCGAATCCTATAGATGAAATACTTTTTTGGCACAATGCTATAAAAAGAGAGTTAAGTGACATAGCTGAGGCCGTCAGGAGGATAAAACTTTTTGGAGATTTTTCTGATTTGTCTGCATTCAACAAGAGGTTGCAGTTCATTGCAGAAGTTTGTATTTTCCATAGCATTGCTGAGGACAAAGTTATATTCCCTTCTCTAGATGCAGAGCTCAATATTGCTCACGAGCACGCAGAAGAAGAAATTCAATTTGACAAGCTTAGGTGCCTCATGGAAAGCATCCAGAGAGCAGGAGCTAACTCATCCACTTCTGAATTTTATATGAAGTTATGCTCTCATGCTGATCAGATAATAGATGGCATACTGAAGCACTTCCAGAATGAGGAACTTCAGGTCCTTCCACTTGCTCGTAAGCATTTTAGCCCCAAAAGACAGAGGGATCTTATGTATCAAAGCTTATGCATGATGCCCTTGAAGTTGATTGAGTGTGTCTTGCCATGGTTGGTAGGATCGCTTACTGAAAAGGAAGCAAGTTCCTTTCTTCAAAATCTTCGAATAGCAGCTCCAGCATCAGATTCAGCACTGGTTACTCTTTTTTCTGGCTGGGCATGCAAAGGTCATTCTGCAAACATTTGTTTGTCTTCAAGTGCAATCAGTTGTCCTCCAAGGACATTGAGTGTAACCGAAGAAGACAGTAGCTATAAGTCACTCTGTTTGTGCACCTCTATGTATTCTGCTAAGGAAGGGCCTTTGTGCAATCAAATAGATGGAGCAGATGATAATCTAAGGCCAGTCAAGAGCGTAAGCTCAGTATCAGAAACTTTTGCTTGCCAGCCTATAGAAAGTATAAATACCCTTCAAAGTTCTAGCAGTAACCAGACATGCTGTGTCCCAGGGTTAGGAGTAAATGACTGTAGCTTAGGAGTGGGTTCTCTTACTCCGGCAAAATCTCTGCGTGGCTTATCTTTTAATCCTTCTACTCTTCCGCTCAACTCCAGTCTTTTTAACTGGGAAACAGATACCAGCTCTACTGACGCTAGTAGTACAACACGACCTATTGATAACATATTTAAGTTCCATAAAGCTATACGTAAGGACTTGGAGTATTTGGATGTTGAATCTGGAAAGCTTAATGGTTGTAATGATGCTTTTATTAGGCATTTTACTGGTAGGTTTCGTTTGTTATGGGGTCTATATAGAGCTCATAGTAATGCAGAGGACGATATTGTATTTCCAGCATTAGAAGCCAAAGAGACTCTTCATAATGTTAGCCATGCTTACACTCTGGATCACAAGCAGGAAGAGAAACTGTTTGAGGATATTTCTTCTGTTCTTTCTGAACTTTCACAACTTAATGAATTGATGAGTATTGGAAATGTGTCTAATGATTCAACTCGAAGTAGCTTGGATTCTTTCAAGTGTGATGATACGCTGAGAAAGTACAATGGACTAACCACGAAACTTCAGGGCATGTGCAAATCCATAAGAGTGACATTGGATCACCATGTTTTCCGCGAAGAACTTGAACTGTGGCCATTGTTTGATAAACATTTTTCTGTGGAAGAACAAGATAAAATTGTTGGTAGAATAATTGGTACTACAGGAGCAGAGGTTCTTCAATCAATGTTGCCGTGGGTAACGGATGCTCTTACTCAGCAAGAGCAAAATAAACTGATGGAAACATGGAAGCAAGCAACCAAAAATACTATGTTCAGTGAGTGGCTTAATGAATGGTGGGATGGAACTCCAGCTGCATCTTCCCATACAGAAACACCAGAAAGTTGCACATCTTTAGGTGCAGAGGCCTCTGAAAGCTTCGAACATACTGATGACACATTTAAACCAGGATGGAAAGATATATTTAGGATGAATCAGAATGAGCTTGAATCAGAAGTAAGAAAAGTTTCTCGGGATTCAACTCTTGATCCAAGAAGAAAAGCATATCTGCTTCAAAATCTAATGACCAGTCGATGGATAGCTTCTCAGCAGAAGTCACCTCAAGCATCTGCAATCGAAGGTTCAAATGGTGAAGATTTGGTTGGATGTTCACCGTCATTTCGTGATTCAGAGAAACAAGTGTTTGGATGTGAGCATTATAAAAGAAATTGCAAATTCCGTGCTGCTTGCTGTGGCAAGTTATTTACATGTAGGTTCTGCCATGATAACGTGAGCGACCATTCAATGGATCGGAAGGCTACATCTGAAATGATGTGCATGCGCTGCTTGAAAATTCAGCCTGTTGGACCAGTCTGCACAACACCTTCCTGTAATGGATTCTCGATGGCACAGTACTATTGTAGTGTTTGCAAGTTTTTTGATGATGAAAGGACTGTTTATCATTGTCCTTCCTGCAATTTATGTCGTGTTGGGAATGGACTTGGTATCGACTTCTTTCATTGCATGACGTGCAATTGTTGCCTGGGGATGAAGTTACTGGATCACAAATGCAGGGAAAAAGGTCTTGAAATAAACTGTCCCATCTGCTGCGACTTTTTGTTTACCTCAAGTGCAACTGTGAGAGCTCTTCCTTGTGGCCATTACATGCATTCAGCTTGCTTCCAGGCATACACTTGCAGTCACTACATCTGCCCAATCTGTAGCAAATCTATGGGAGATATGGCGGTTTATTTTGGCATGCTTGATGCGCTACTGGCTTCCGAGGAACTCCCAGAAGAATACAGGGATCGCTGTCAGGACATACTGTGCAACGACTGTGATAGGAAGGGGACGGCGCTGTTCCACTGGCTGTACCATAAGTGCGGGACTTGTGGATCCTATAACACCAAGGTTATCAGGGTAGACTCCAACTCCCACTGCTTAACATCACACAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hemerythrin zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

1230

Amino Acids

137.84

Weight (kDa)

5.65

Isoelectric Point (pI)

53.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hemerythrin	PF01814	42 - 171	1.1e-09	Hemerythrin HHE cation binding domain
Hemerythrin	PF01814	637 - 797	9.7e-06	Hemerythrin HHE cation binding domain
zf-CHY	PF05495	981 - 1049	9.6e-19	CHY zinc finger
zf-RING_2	PF13639	1109 - 1151	2.1e-06	Ring finger domain
zinc_ribbon_6	PF14599	1157 - 1214	6.7e-23	Zinc-ribbon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18290
fragaria_vesca	FvH4_4g26990 FvH4_4g26990 FvH4_4g26990 FvH4_4g26990 FvH4_5g11860 FvH4_5g11860 FvH4_5g11860
malus_domestica	MD06G1217900.v1.1 MD14G1228100.v1.1 MD16G1039900.v1.1
prunus_persica	Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1
pyrus_communis	pycom06g19410 pycom14g18950 pycom16g03460
rosa_chinensis	RchiOBHm_Chr4g0434791 RchiOBHm_Chr7g0185731
rosa_laevigata	RLG00000004888 RLG00000006649
rosa_multiflora	Rmu_sc0000179.1_g000001 Rmu_sc0002952.1_g000002
rosa_roxburghii	Rroxscaffold_3G00268670 Rroxscaffold_5G00376000
rosa_rugosa	Rorug04G0278300 Rorug04G0278400 Rorug06G0470900
rosa_samantha	Rh4AG334900 Rh4CG357800 Rh4DG337300 Rh7AG071600 Rh7BG076200 Rh7DG076200
rosa_wichuraiana	Rw4G029200 Rw7G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	261, 2952
Acc16I	TGCGCA	1 cut(s)	647
Acc36I	ACCTGC	1 cut(s)	682
AccB1I	GGYRCC	2 cut(s)	7, 1119
AccB7I	CCANNNNNTGG	3 cut(s)	17, 2826, 3037
AccBSI	CCGCTC	2 cut(s)	61, 1843
AccI	GTMKAC	1 cut(s)	3654
AccII	CGCG	3 cut(s)	252, 788, 2370
AcoI	YGGCCR	2 cut(s)	2387, 3388
AcsI	RAATTY	6 cut(s)	216, 593, 1098, 1169, 2966, 3084
AcuI	CTGAAG	4 cut(s)	110, 1229, 1241, 2303
AfaI	GTAC	8 cut(s)	445, 653, 831, 1900, 2297, 2452, 3146, 3605
AfiI	CCNNNNNNNGG	9 cut(s)	17, 23, 274, 552, 936, 1237, 1754, 2826, 3037
AflIII	ACRYGT	2 cut(s)	820, 2999
AjiI	CACGTC	1 cut(s)	3261
AjnI	CCWGG	7 cut(s)	16, 704, 800, 1747, 2698, 3273, 3414
AjuI	GAANNNNNNNTTGG	2 cut(s)	2877, 2909
AloI	GAACNNNNNNTCC	8 cut(s)	348, 380, 486, 518, 1233, 1265, 2451, 2483
Alw21I	GWGCWC	7 cut(s)	98, 159, 1068, 1086, 1577, 2059, 3379
Alw26I	GTCTC	1 cut(s)	2102
Alw44I	GTGCAC	1 cut(s)	1573
AlwNI	CAGNNNCTG	1 cut(s)	562
Ama87I	CYCGRG	1 cut(s)	2763
AoxI	GGCC	9 cut(s)	163, 183, 276, 927, 1601, 1643, 2387, 2658, 3388
ApaLI	GTGCAC	1 cut(s)	1573
ApeKI	GCWGC	7 cut(s)	388, 688, 1415, 2606, 2975, 3075, 3339
ApoI	RAATTY	6 cut(s)	216, 593, 1098, 1169, 2966, 3084
Asp700I	GAANNNNTTC	5 cut(s)	337, 597, 1098, 1226, 2758
AspLEI	GCGC	6 cut(s)	254, 648, 788, 3075, 3502, 3589
AspS9I	GGNCC	4 cut(s)	47, 1249, 1601, 3098
AsuHPI	GGTGA	5 cut(s)	328, 2348, 2841, 2894, 2895
AsuII	TTCGAA	3 cut(s)	860, 1408, 2673
AvaI	CYCGRG	1 cut(s)	2763
AvaII	GGWCC	3 cut(s)	47, 1249, 3098
BaeGI	GKGCMC	1 cut(s)	1577
BalI	TGGCCA	2 cut(s)	2389, 3390
BamHI	GGATCC	1 cut(s)	3626
BanI	GGYRCC	2 cut(s)	7, 1119
BanII	GRGCYC	5 cut(s)	98, 159, 1068, 2059, 3379
BauI	CACGAG	2 cut(s)	350, 1079
BbsI	GAAGAC	3 cut(s)	493, 1488, 1551
Bbv12I	GWGCWC	7 cut(s)	98, 159, 1068, 1086, 1577, 2059, 3379
BbvCI	CCTCAGC	2 cut(s)	924, 1029
BbvI	GCAGC	7 cut(s)	375, 700, 1427, 2593, 2962, 3062, 3326
BceAI	ACGGC	4 cut(s)	178, 914, 2470, 3600
BciT130I	CCWGG	7 cut(s)	18, 706, 802, 1749, 2700, 3275, 3416
BclI	TGATCA	1 cut(s)	1198
BcoDI	GTCTC	1 cut(s)	2102
BfaI	CTAG	5 cut(s)	305, 392, 1055, 1722, 1893
BfmI	CTRYAG	6 cut(s)	686, 867, 1692, 1768, 2454, 3451
BfoI	RGCGCY	1 cut(s)	3590
BfuAI	ACCTGC	1 cut(s)	682
BglI	GCCNNNNNGGC	1 cut(s)	182
BmcAI	AGTACT	2 cut(s)	653, 3146
Bme1390I	CCNGG	7 cut(s)	18, 706, 802, 1749, 2700, 3275, 3416
Bme18I	GGWCC	3 cut(s)	47, 1249, 3098
BmeT110I	CYCGRG	1 cut(s)	2763
BmgBI	CACGTC	1 cut(s)	3261
BmgT120I	GGNCC	4 cut(s)	47, 1249, 1601, 3098
BmiI	GGNNCC	6 cut(s)	9, 49, 205, 451, 1121, 3628
BmrFI	CCNGG	7 cut(s)	18, 706, 802, 1749, 2700, 3275, 3416
BmrI	ACTGGG	1 cut(s)	1873
BmsI	GCATC	9 cut(s)	1048, 1143, 1311, 1433, 1990, 2488, 2618, 2867, 3487
BmuI	ACTGGG	1 cut(s)	1873
BpiI	GAAGAC	3 cut(s)	493, 1488, 1551
BplI	GAGNNNNNCTC	2 cut(s)	85, 117
BpmI	CTGGAG	5 cut(s)	251, 468, 1404, 1834, 2586
Bpu10I	CCTNAGC	6 cut(s)	52, 924, 1029, 1115, 1593, 1774
Bpu14I	TTCGAA	3 cut(s)	860, 1408, 2673
BpuEI	CTTGAG	3 cut(s)	676, 2838, 3343
BsaAI	YACGTR	1 cut(s)	1946
BsaBI	GATNNNNATC	2 cut(s)	119, 1635
BsaWI	WCCGGW	1 cut(s)	423
BsaXI	ACNNNNNCTCC	6 cut(s)	348, 378, 2451, 2481, 3647, 3677
Bsc4I	CCNNNNNNNGG	9 cut(s)	17, 23, 274, 552, 936, 1237, 1754, 2826, 3037
Bse3DI	GCAATG	4 cut(s)	268, 996, 1023, 3250
Bse8I	GATNNNNATC	2 cut(s)	119, 1635
BseBI	CCWGG	7 cut(s)	18, 706, 802, 1749, 2700, 3275, 3416
BseJI	GATNNNNATC	2 cut(s)	119, 1635
BseLI	CCNNNNNNNGG	9 cut(s)	17, 23, 274, 552, 936, 1237, 1754, 2826, 3037
BseMI	GCAATG	4 cut(s)	268, 996, 1023, 3250
BseMII	CTCAG	6 cut(s)	915, 1020, 1677, 2279, 2522, 2852
BseRI	GAGGAG	2 cut(s)	428, 548
BseSI	GKGCMC	1 cut(s)	1577
BseXI	GCAGC	7 cut(s)	375, 700, 1427, 2593, 2962, 3062, 3326
BseYI	CCCAGC	1 cut(s)	1458
BsgI	GTGCAG	2 cut(s)	2673, 3091
Bsh1236I	CGCG	3 cut(s)	252, 788, 2370
BshFI	GGCC	9 cut(s)	165, 185, 278, 929, 1603, 1645, 2389, 2660, 3390
BshNI	GGYRCC	2 cut(s)	7, 1119
BsiHKAI	GWGCWC	7 cut(s)	98, 159, 1068, 1086, 1577, 2059, 3379
BsiHKCI	CYCGRG	1 cut(s)	2763
BsiSI	CCGG	2 cut(s)	424, 1796
BslFI	GGGAC	4 cut(s)	1730, 3315, 3595, 3631
BslI	CCNNNNNNNGG	9 cut(s)	17, 23, 274, 552, 936, 1237, 1754, 2826, 3037
BsmAI	GTCTC	1 cut(s)	2102
BsmFI	GGGAC	4 cut(s)	1730, 3315, 3595, 3631
BsmI	GAATGC	2 cut(s)	974, 3400
BsnI	GGCC	9 cut(s)	165, 185, 278, 929, 1603, 1645, 2389, 2660, 3390
BsoBI	CYCGRG	1 cut(s)	2763
Bsp119I	TTCGAA	3 cut(s)	860, 1408, 2673
Bsp1286I	GDGCHC	7 cut(s)	98, 159, 1068, 1086, 1577, 2059, 3379
Bsp19I	CCATGG	3 cut(s)	180, 852, 1352
BspANI	GGCC	9 cut(s)	165, 185, 278, 929, 1603, 1645, 2389, 2660, 3390
BspCNI	CTCAG	6 cut(s)	916, 1021, 1676, 2280, 2521, 2851
BspFNI	CGCG	3 cut(s)	252, 788, 2370
BspLI	GGNNCC	6 cut(s)	9, 49, 205, 451, 1121, 3628
BspMAI	CTGCAG	1 cut(s)	690
BspMI	ACCTGC	1 cut(s)	682
BspQI	GCTCTTC	2 cut(s)	86, 3384
BspT104I	TTCGAA	3 cut(s)	860, 1408, 2673
BspT107I	GGYRCC	2 cut(s)	7, 1119
BsrBI	CCGCTC	2 cut(s)	61, 1843
BsrDI	GCAATG	4 cut(s)	268, 996, 1023, 3250
BssSI	CACGAG	2 cut(s)	350, 1079
BssT1I	CCWWGG	7 cut(s)	180, 483, 546, 852, 1352, 1520, 3639
Bst2BI	CACGAG	2 cut(s)	350, 1079
Bst2UI	CCWGG	7 cut(s)	18, 706, 802, 1749, 2700, 3275, 3416
Bst6I	CTCTTC	8 cut(s)	86, 483, 616, 669, 1842, 2118, 2151, 3384
BstAPI	GCANNNNNTGC	3 cut(s)	3075, 3261, 3270
BstBAI	YACGTR	1 cut(s)	1946
BstBI	TTCGAA	3 cut(s)	860, 1408, 2673
BstDSI	CCRYGG	5 cut(s)	180, 602, 852, 1352, 2486
BstFNI	CGCG	3 cut(s)	252, 788, 2370
BstH2I	RGCGCY	1 cut(s)	3590
BstHHI	GCGC	6 cut(s)	254, 648, 788, 3075, 3502, 3589
BstMAI	GTCTC	1 cut(s)	2102
BstNI	CCWGG	7 cut(s)	18, 706, 802, 1749, 2700, 3275, 3416
BstNSI	RCATGY	7 cut(s)	1467, 1740, 2329, 3003, 3073, 3400, 3493
BstSCI	CCNGG	7 cut(s)	16, 704, 800, 1747, 2698, 3273, 3414
BstSFI	CTRYAG	6 cut(s)	686, 867, 1692, 1768, 2454, 3451
BstSLI	GKGCMC	1 cut(s)	1577
BstSNI	TACGTA	1 cut(s)	1946
BstUI	CGCG	3 cut(s)	252, 788, 2370
BstV1I	GCAGC	7 cut(s)	375, 700, 1427, 2593, 2962, 3062, 3326
BstV2I	GAAGAC	3 cut(s)	493, 1488, 1551
BstX2I	RGATCY	2 cut(s)	1294, 3626
BstXI	CCANNNNNNTGG	1 cut(s)	853
BstYI	RGATCY	2 cut(s)	1294, 3626
BsuRI	GGCC	9 cut(s)	165, 185, 278, 929, 1603, 1645, 2389, 2660, 3390
BtgI	CCRYGG	5 cut(s)	180, 602, 852, 1352, 2486
BtrI	CACGTC	1 cut(s)	3261
BtsI	GCAGTG	1 cut(s)	3667
BtsIMutI	CAGTG	4 cut(s)	1436, 2575, 3595, 3667
BveI	ACCTGC	1 cut(s)	682
CaiI	CAGNNNCTG	1 cut(s)	562
CfoI	GCGC	6 cut(s)	254, 648, 788, 3075, 3502, 3589
Cfr13I	GGNCC	4 cut(s)	47, 1249, 1601, 3098
CseI	GACGC	1 cut(s)	1898
Csp6I	GTAC	8 cut(s)	444, 652, 830, 1899, 2296, 2451, 3145, 3604
CviQI	GTAC	8 cut(s)	444, 652, 830, 1899, 2296, 2451, 3145, 3604
DraI	TTTAAA	1 cut(s)	2695
EaeI	YGGCCR	2 cut(s)	2387, 3388
Eam1104I	CTCTTC	8 cut(s)	86, 483, 616, 669, 1842, 2118, 2151, 3384
EarI	CTCTTC	8 cut(s)	86, 483, 616, 669, 1842, 2118, 2151, 3384
EciI	GGCGGA	1 cut(s)	196
Ecl136II	GAGCTC	5 cut(s)	96, 157, 1066, 2057, 3377
Eco105I	TACGTA	1 cut(s)	1946
Eco130I	CCWWGG	7 cut(s)	180, 483, 546, 852, 1352, 1520, 3639
Eco147I	AGGCCT	1 cut(s)	2660
Eco24I	GRGCYC	5 cut(s)	98, 159, 1068, 2059, 3379
Eco47I	GGWCC	3 cut(s)	47, 1249, 3098
Eco53kI	GAGCTC	5 cut(s)	96, 157, 1066, 2057, 3377
Eco57I	CTGAAG	4 cut(s)	110, 1229, 1241, 2303
Eco88I	CYCGRG	1 cut(s)	2763
EcoICRI	GAGCTC	5 cut(s)	96, 157, 1066, 2057, 3377
EcoO109I	RGGNCCY	2 cut(s)	1249, 1601
EcoRI	GAATTC	1 cut(s)	216
EcoRII	CCWGG	7 cut(s)	16, 704, 800, 1747, 2698, 3273, 3414
EcoT14I	CCWWGG	7 cut(s)	180, 483, 546, 852, 1352, 1520, 3639
EcoT22I	ATGCAT	2 cut(s)	1319, 3402
EcoT38I	GRGCYC	5 cut(s)	98, 159, 1068, 2059, 3379
ErhI	CCWWGG	7 cut(s)	180, 483, 546, 852, 1352, 1520, 3639
FalI	AAGNNNNNCTT	4 cut(s)	666, 698, 2097, 2129
FaqI	GGGAC	4 cut(s)	1730, 3315, 3595, 3631
FauI	CCCGC	2 cut(s)	66, 3608
FauNDI	CATATG	1 cut(s)	477
FbaI	TGATCA	1 cut(s)	1198
FblI	GTMKAC	1 cut(s)	3654
FriOI	GRGCYC	5 cut(s)	98, 159, 1068, 2059, 3379
FspBI	CTAG	5 cut(s)	305, 392, 1055, 1722, 1893
FspI	TGCGCA	1 cut(s)	647
GlaI	GCGC	6 cut(s)	253, 647, 787, 3074, 3501, 3588
GsaI	CCCAGC	1 cut(s)	1462
GsuI	CTGGAG	5 cut(s)	251, 468, 1404, 1834, 2586
HaeII	RGCGCY	1 cut(s)	3590
HaeIII	GGCC	9 cut(s)	165, 185, 278, 929, 1603, 1645, 2389, 2660, 3390
HapII	CCGG	2 cut(s)	424, 1796
HgaI	GACGC	1 cut(s)	1898
HhaI	GCGC	6 cut(s)	254, 648, 788, 3075, 3502, 3589
Hin6I	GCGC	6 cut(s)	252, 646, 786, 3073, 3500, 3587
HinP1I	GCGC	6 cut(s)	252, 646, 786, 3073, 3500, 3587
HincII	GTYRAC	1 cut(s)	580
HindII	GTYRAC	1 cut(s)	580
HindIII	AAGCTT	6 cut(s)	540, 680, 1112, 1309, 1982, 2668
HpaI	GTTAAC	1 cut(s)	580
HpaII	CCGG	2 cut(s)	424, 1796
HphI	GGTGA	5 cut(s)	328, 2348, 2841, 2894, 2895
Hpy166II	GTNNAC	6 cut(s)	580, 826, 1575, 2903, 3354, 3655
Hpy8I	GTNNAC	6 cut(s)	580, 826, 1575, 2903, 3354, 3655
Hpy99I	CGWCG	2 cut(s)	164, 632
HpyCH4IV	ACGT	6 cut(s)	335, 820, 828, 1945, 3021, 3260
HpySE526I	ACGT	6 cut(s)	335, 820, 828, 1945, 3021, 3260
HspAI	GCGC	6 cut(s)	252, 646, 786, 3073, 3500, 3587
Ksp22I	TGATCA	1 cut(s)	1198
KspAI	GTTAAC	1 cut(s)	580
LguI	GCTCTTC	2 cut(s)	86, 3384
LmnI	GCTCC	7 cut(s)	209, 449, 535, 1148, 1423, 1625, 2459
Lsp1109I	GCAGC	7 cut(s)	375, 700, 1427, 2593, 2962, 3062, 3326
LweI	GCATC	9 cut(s)	1048, 1143, 1311, 1433, 1990, 2488, 2618, 2867, 3487
MaeI	CTAG	5 cut(s)	305, 392, 1055, 1722, 1893
MaeII	ACGT	6 cut(s)	335, 820, 828, 1945, 3021, 3260
MbiI	CCGCTC	2 cut(s)	61, 1843
MfeI	CAATTG	1 cut(s)	3265
MflI	RGATCY	2 cut(s)	1294, 3626
MhlI	GDGCHC	7 cut(s)	98, 159, 1068, 1086, 1577, 2059, 3379
MlsI	TGGCCA	2 cut(s)	2389, 3390
MluNI	TGGCCA	2 cut(s)	2389, 3390
MlyI	GAGTC	3 cut(s)	389, 2104, 3650
MmeI	TCCRAC	4 cut(s)	651, 2875, 3076, 3684
Mox20I	TGGCCA	2 cut(s)	2389, 3390
Mph1103I	ATGCAT	2 cut(s)	1319, 3402
MroXI	GAANNNNTTC	5 cut(s)	337, 597, 1098, 1226, 2758
MscI	TGGCCA	2 cut(s)	2389, 3390
MslI	CAYNNNNRTG	4 cut(s)	1580, 2064, 2342, 2682
Msp20I	TGGCCA	2 cut(s)	2389, 3390
MspA1I	CMGCKG	2 cut(s)	212, 2606
MspI	CCGG	2 cut(s)	424, 1796
MspR9I	CCNGG	7 cut(s)	18, 706, 802, 1749, 2700, 3275, 3416
MunI	CAATTG	1 cut(s)	3265
Mva1269I	GAATGC	2 cut(s)	974, 3400
MvaI	CCWGG	7 cut(s)	18, 706, 802, 1749, 2700, 3275, 3416
MvnI	CGCG	3 cut(s)	252, 788, 2370
NcoI	CCATGG	3 cut(s)	180, 852, 1352
NdeI	CATATG	1 cut(s)	477
NlaIV	GGNNCC	6 cut(s)	9, 49, 205, 451, 1121, 3628
NmeAIII	GCCGAG	2 cut(s)	77, 304
NmuCI	GTSAC	5 cut(s)	914, 1560, 2344, 2847, 3431
NsbI	TGCGCA	1 cut(s)	647
NsiI	ATGCAT	2 cut(s)	1319, 3402
NspI	RCATGY	7 cut(s)	1467, 1740, 2329, 3003, 3073, 3400, 3493
NspV	TTCGAA	3 cut(s)	860, 1408, 2673
PaeI	GCATGC	3 cut(s)	1467, 3073, 3493
PasI	CCCWGGG	1 cut(s)	1748
PceI	AGGCCT	1 cut(s)	2660
PciI	ACATGT	1 cut(s)	2999
PciSI	GCTCTTC	2 cut(s)	86, 3384
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PctI	GAATGC	2 cut(s)	974, 3400
PdmI	GAANNNNTTC	5 cut(s)	337, 597, 1098, 1226, 2758
PflFI	GACNNNGTC	1 cut(s)	287
PflMI	CCANNNNNTGG	3 cut(s)	17, 2826, 3037
PfoI	TCCNGGA	1 cut(s)	800
PleI	GAGTC	3 cut(s)	389, 2104, 3650
PpsI	GAGTC	3 cut(s)	389, 2104, 3650
Ppu21I	YACGTR	1 cut(s)	1946
PpuMI	RGGWCCY	1 cut(s)	1249
PscI	ACATGT	1 cut(s)	2999
PsiI	TTATAA	2 cut(s)	261, 2952
Psp124BI	GAGCTC	5 cut(s)	98, 159, 1068, 2059, 3379
Psp5II	RGGWCCY	1 cut(s)	1249
Psp6I	CCWGG	7 cut(s)	16, 704, 800, 1747, 2698, 3273, 3414
PspFI	CCCAGC	1 cut(s)	1458
PspGI	CCWGG	7 cut(s)	16, 704, 800, 1747, 2698, 3273, 3414
PspN4I	GGNNCC	6 cut(s)	9, 49, 205, 451, 1121, 3628
PspPI	GGNCC	4 cut(s)	47, 1249, 1601, 3098
PspPPI	RGGWCCY	1 cut(s)	1249
PstI	CTGCAG	1 cut(s)	690
PstNI	CAGNNNCTG	1 cut(s)	562
PsuI	RGATCY	2 cut(s)	1294, 3626
PsyI	GACNNNGTC	1 cut(s)	287
PvuII	CAGCTG	1 cut(s)	2606
RsaI	GTAC	8 cut(s)	445, 653, 831, 1900, 2297, 2452, 3146, 3605
RsaNI	GTAC	8 cut(s)	444, 652, 830, 1899, 2296, 2451, 3145, 3604
RseI	CAYNNNNRTG	4 cut(s)	1580, 2064, 2342, 2682
SacI	GAGCTC	5 cut(s)	98, 159, 1068, 2059, 3379
SapI	GCTCTTC	2 cut(s)	86, 3384
Sau96I	GGNCC	4 cut(s)	47, 1249, 1601, 3098
ScaI	AGTACT	2 cut(s)	653, 3146
SchI	GAGTC	3 cut(s)	389, 2104, 3650
ScrFI	CCNGG	7 cut(s)	18, 706, 802, 1749, 2700, 3275, 3416
SduI	GDGCHC	7 cut(s)	98, 159, 1068, 1086, 1577, 2059, 3379
SfaNI	GCATC	9 cut(s)	1048, 1143, 1311, 1433, 1990, 2488, 2618, 2867, 3487
SfcI	CTRYAG	6 cut(s)	686, 867, 1692, 1768, 2454, 3451
SfuI	TTCGAA	3 cut(s)	860, 1408, 2673
SinI	GGWCC	3 cut(s)	47, 1249, 3098
SmiMI	CAYNNNNRTG	4 cut(s)	1580, 2064, 2342, 2682
SmlI	CTYRAG	3 cut(s)	655, 2853, 3358
SmoI	CTYRAG	3 cut(s)	655, 2853, 3358
SnaBI	TACGTA	1 cut(s)	1946
SphI	GCATGC	3 cut(s)	1467, 3073, 3493
SseBI	AGGCCT	1 cut(s)	2660
SspI	AATATT	1 cut(s)	1072
SspMI	CTAG	5 cut(s)	305, 392, 1055, 1722, 1893
SstI	GAGCTC	5 cut(s)	98, 159, 1068, 2059, 3379
StuI	AGGCCT	1 cut(s)	2660
StyD4I	CCNGG	7 cut(s)	16, 704, 800, 1747, 2698, 3273, 3414
StyI	CCWWGG	7 cut(s)	180, 483, 546, 852, 1352, 1520, 3639
TaiI	ACGT	6 cut(s)	338, 823, 831, 1948, 3024, 3263
TatI	WGTACW	5 cut(s)	443, 651, 1898, 2295, 3144
TauI	GCSGC	3 cut(s)	8, 179, 212
TscAI	CASTG	4 cut(s)	1443, 2575, 3602, 3674
TseFI	GTSAC	5 cut(s)	914, 1560, 2344, 2847, 3431
TseI	GCWGC	7 cut(s)	388, 688, 1415, 2606, 2975, 3075, 3339
Tsp45I	GTSAC	5 cut(s)	914, 1560, 2344, 2847, 3431
TspGWI	ACGGA	4 cut(s)	63, 591, 2510, 2960
TspRI	CASTG	4 cut(s)	1443, 2575, 3602, 3674
Tth111I	GACNNNGTC	1 cut(s)	287
Van91I	CCANNNNNTGG	3 cut(s)	17, 2826, 3037
VneI	GTGCAC	1 cut(s)	1573
VpaK11BI	GGWCC	3 cut(s)	47, 1249, 3098
XapI	RAATTY	6 cut(s)	216, 593, 1098, 1169, 2966, 3084
XbaI	TCTAGA	2 cut(s)	304, 1054
XceI	RCATGY	7 cut(s)	1467, 1740, 2329, 3003, 3073, 3400, 3493
XmiI	GTMKAC	1 cut(s)	3654
XmnI	GAANNNNTTC	5 cut(s)	337, 597, 1098, 1226, 2758
XspI	CTAG	5 cut(s)	305, 392, 1055, 1722, 1893
ZrmI	AGTACT	2 cut(s)	653, 3146
Zsp2I	ATGCAT	2 cut(s)	1319, 3402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.