Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0002376

Physical Location & Seq

Genomic Coordinates Reverse (-)

5794295 .. 5802651

Gene Structure 8357 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ16577

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3717 bp

ATGGCGACGCCGTTAACGGTGGACGGAGGCGGAGGGCTGGCGGTGCTGGCAAGCTCCGTCAACAAAGTCGATTCTCCGAGTAAGGGGCGCTTGACTAGCTCGTCGTCGTCGGAGGAGGAGGAGAGGTCGCCGATTCTGATATTCTTGTTCTTTCACAAGGCAATTCGGAAGGAGCTCGATGCGCTTCACCGATTGGCCATGGCTTTCGCCAGCGGAAAGCAGACGGATATCAGGCCCTTGCTGGAGCGGTACCATTTCTTGAGATCGATTTACAAGCACCACTCCAATGCCGAAGACGAGGTAATCTTTCCAGCGCTTGATATACGTGTGAAGAATGTAGCACAAACATACTCGCTTGAACACAAGGGCGAAACCAATCTTTTTGATCATCTATTTGAACTGCTAAGTTCTAATGCACAAAGTGATGACAGTTTCACAAGGGAGTTAGCATCTTGTACAGGAGCCTTACAAACATCAGTTAGCCAACACATGGCAAAGGAGGAGGAACAGGTCTTACCCTTGCTGATTGAGAAGTTTTCAGTTGAGGAGCAGGCATCTCTAGTCTGGCAGTTTCTATGCAGTATTCCTGTGAATATGATGGCCGAGTTTCTTCCGTGGCTTTCATCATCAGTTTCACCTGATGAATATCAGGATTTGCGGAAATGCTTATGCAAGATTGTTCCAGAGGAAAAGCTTCTTCAACAAGTCATTTTCACCTGGATGGAAGGTAGTACGAGCAATAATGTGGTTAAAAGTTATCATGATTCTCCTCAGTTCCAATGTTGCGTGGAATCTGGTGCTAGCACATCAAGTCTACATATAGAGAAGATAAATTGTCCATGTGAGTGTAGGACTGGGAAAAGGAAGTATTTAGAGTCAAGTACTGATGTTTCTGATACCACTGGGGGCCATCCAATTAATGAAATACTGCTTTGGCATAATGCCATTAAAAAGGAGTTAAATGAGATAGCCGAGGAGGCTAGGAAGATACAACTGTCTGGAGATTTTACTAATCTATCAGCTTTCAATGAGAGGTTGCAATTTGTTGCTGAAGTTTGCATTTTTCACAGTATTGCCGAGGACAAGGTCATATTTCCTGCAGTAGATGGAAAGATATCCTTTTTCCAAGAGCATGCTGAAGAAGAAAGTCAGTTCAATGAGTTCAGGTGTCTGATTGAAAACATTCAAAGTGCAGGAGCTATCTCTACTTCAGCTGATTTTTATGCAGAGCTGTGCTCACACGCTGATCAAATAATAGAAACTATACAGAAGCACTTCAGCAATGAAGAAGTGCAGGTTCTTCCACTTGCTCGAAAGCACTTCAGCTTCAAAAGACAGCGGGAACTATTGTACCAGAGTTTATGCATGATGCCCTTGAAATTGATCGAGCGTGTTTTTCCATGGCTAGTCAGATTGTTGACTGAAGATGAAATGAAGAATATTCTCAAAAACATGCAGTTGGCAGCTCCAGTCCCTGATTCAGCTCTGGTAACACTTTTTTCTGGATGGGCATGCAAGGCTCGTAATCAGGGTTCATGCTTGTCTTCAAGTGAACTTGGTTGCTGTCCTGTTAAAAGTTTTACTGATATTGAAGAAGATTTTGTTCGACCCGCATGTGCTTGTGCTTCTGGATTGTCTGCTAGAGAGAGACTGGTATCAGCTAAAGTAAATAATGTGAAAAGGCTGGTCAAACGAAATGTTTCAGTGCCGCGCAAAAACAGTGATACCCCTGATCAATGTTGTACTGATCAATCTTGCCGTGTCCCGGGTTTAGGAGTAAACAACACTAATCTTGGATCGAGTTCTCAATTCGTGGCCAAGTCTTTGCGGTCCTTGTCTTTCAGCTCTTCTGCCCCATCTCTTAATTCCAGTCTTTTTGCCTGGGAAACAGATAATTCTTCCTTTGATATTGGTTGTGGAGAACGACCTATTGATACTATTTTTAAGTTTCATAAAGCCATTCGCAAAGACTTGGAGTATTTAGATATTGAATCTGGAAAGCTCGGTAATGGTGATGAAGCCACACTTCGGCAGTTTATAGGAAGATTCCGTTTGCTATGGGGCTTATACAGAGCACACAGTAATGCTGAGGATGATATAGTGTTTCCTGCATTGGAATCCAAAGAGGCACTTCATAATGTGAGCCATTCATACACACTGGACCATAAACAGGAGGAAAAATTATTTGAAGACATCTCTCGTGTTCTTTCTGAACTTTCTGACCTTCATGAAAGCATGGAAAAGACTCATATGGATGAGGATTTAGCAGGAAGTAGCATGAATTTTTCTGCCGCAAATGGTGTGAACTATACTAGGAATTACAATGAGCTGGCTACAAAGCTTCAAGGGATGTGCAAGTCTATAAAAGTGACACTAGATCATCACATTTTCAGGGAAGAACTTGAGTTGTGGCCATTATTTGGCAAACACTTCACTGTTGAGGAGCAAGACAAAATAGTCGGGCGGATTATTGGGACCACGGGTGCTGAGGTGCTCCAATCAATGTTACCTTGGGTAACTTCTGCACTTACCCAGGATGAACAGAATAAATTGATGGACACATGGAAGCAAGCAACTAAAAATACTATGTTTAATGAGTGGCTTAATGAATGCATGAAAGGATCTTCAGAATCAACTTCACGAACTGAGACAAGGGAAAGTAACATTTCTCAAAAAGGTGTTGAATTTCAAGAAAGCTTGGACCAGACGGACCAGATGTTCAAGCCTGGTTGGAAGGACATATTCCGTATGAATCAAAATGAACTAGAGTCAGAGATCAGAAAGGTTTATCGTGATGCGACTCTTGATCCAAGGAGAAAGGCATATCTTGTGCAAAATCTTATGACTAGCCGCTGGATAGCTACTCAGCAGAAGTTACCTCAAGCAACAGGTGGAGAGTCTTCTAATGGTGAAGATGTATTTGGACGCTCACCTTCATACCGTGATGTGGAGAAAAAAGTATTTGGGTGTGAACACTACAAAAGAAACTGTAAACTCCGAGCTGCTTGTTGTGGCAAGTTATTTGCATGTAGATTTTGCCATGACAATGTGAGCGACCATTCAATGGATAGGAAAGCAACATCGGAAATTATGTGCATGCGCTGCCTAAAAATTCAGCCAGTTGGGCCCATATGTACTACACCTTCATGCAATGAACTATCTATGGCAAAGTATTATTGCAATATCTGCAAATTTTTTGATGATGAAAGGACTGTATATCATTGCCCGTTTTGCAATTTATGCCGCCTTGGGAAGGGTCTTGGCGTTGACTTTTTTCATTGCATGACATGCAATTGTTGCCTGGGGATAAAGTTGGTGAATCACAAATGCCTGGAGAAAAGTTTAGAAACAAATTGCCCTATCTGCTGTGATTTCTTGTTCACATCCAGTGCAACAGTTAGGGCTTTGCCATGTGGCCATTACATGCATTCAGCTTGCTTTCAGGCCTACACTTGCAGTCACTATACTTGCCCAATTTGTAGCAAGTCTCTGGGAGATATGGCGGTTTACTTTGGCATGCTTGATGCCTTATTGGCTGCTGAGCAGCTTCCAGAGGAATACCAGAACCGTCAGCAGGACATCCTTTGTAATGATTGTGATCGAAAGGGTACCTCACGCTTTCACTGGCTATATCACAAGTGCGGTAACTGTGGATCATACAATACCCGGGTGATCAAGGGTGAGGCAACAAATACCGACTGTCCTATGTCAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hemerythrin zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

1238

Amino Acids

139.92

Weight (kDa)

5.87

Isoelectric Point (pI)

52.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hemerythrin	PF01814	50 - 175	2.1e-11	Hemerythrin HHE cation binding domain
Hemerythrin	PF01814	643 - 805	5e-07	Hemerythrin HHE cation binding domain
zf-CHY	PF05495	990 - 1058	5.1e-19	CHY zinc finger
zf-RING_2	PF13639	1118 - 1160	4.4e-07	Ring finger domain
zinc_ribbon_6	PF14599	1166 - 1223	4.3e-24	Zinc-ribbon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18290
fragaria_vesca	FvH4_4g26990 FvH4_4g26990 FvH4_4g26990 FvH4_4g26990 FvH4_5g11860 FvH4_5g11860 FvH4_5g11860
malus_domestica	MD06G1217900.v1.1 MD14G1228100.v1.1 MD16G1039900.v1.1
prunus_persica	Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.1G314300_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1 Prupe.5G223100_v2.0.a1
pyrus_communis	pycom06g19410 pycom14g18950 pycom16g03460
rosa_chinensis	RchiOBHm_Chr4g0434791 RchiOBHm_Chr7g0185731
rosa_laevigata	RLG00000004888 RLG00000006649
rosa_multiflora	Rmu_sc0000179.1_g000001 Rmu_sc0002952.1_g000002
rosa_roxburghii	Rroxscaffold_3G00268670 Rroxscaffold_5G00376000
rosa_rugosa	Rorug04G0278300 Rorug04G0278400 Rorug06G0470900
rosa_samantha	Rh4AG334900 Rh4CG357800 Rh4DG337300 Rh7AG071600 Rh7BG076200 Rh7DG076200
rosa_wichuraiana	Rw4G029200 Rw7G006360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	100, 2456
Acc36I	ACCTGC	1 cut(s)	1285
Acc65I	GGTACC	2 cut(s)	249, 3608
AccB1I	GGYRCC	2 cut(s)	249, 3608
AccB7I	CCANNNNNTGG	3 cut(s)	490, 2420, 3064
AccBSI	CCGCTC	1 cut(s)	247
AccI	GTMKAC	1 cut(s)	814
AccII	CGCG	1 cut(s)	1711
AclWI	GGATC	4 cut(s)	1804, 2629, 2801, 3661
AcoI	YGGCCR	5 cut(s)	195, 600, 1815, 2411, 3415
AcsI	RAATTY	4 cut(s)	2281, 2684, 3111, 3191
AcuI	CTGAAG	7 cut(s)	1071, 1158, 1194, 1261, 1306, 1443, 2610
AcyI	GRCGYC	1 cut(s)	8
AdeI	CACNNNGTG	1 cut(s)	422
AfaI	GTAC	8 cut(s)	251, 457, 733, 883, 1352, 1744, 3136, 3610
AfeI	AGCGCT	1 cut(s)	315
AflIII	ACRYGT	1 cut(s)	325
AjnI	CCWGG	6 cut(s)	716, 1880, 2532, 2725, 3300, 3330
AjuI	GAANNNNNNNTTGG	2 cut(s)	2904, 2936
AloI	GAACNNNNNNTCC	2 cut(s)	2702, 2734
Alw21I	GWGCWC	4 cut(s)	177, 1238, 2077, 2496
Alw26I	GTCTC	3 cut(s)	1642, 2642, 3492
AlwI	GGATC	4 cut(s)	1804, 2629, 2801, 3661
AlwNI	CAGNNNCTG	1 cut(s)	1475
Ama87I	CYCGRG	2 cut(s)	1765, 3666
Aor51HI	AGCGCT	1 cut(s)	315
AoxI	GGCC	9 cut(s)	195, 233, 600, 907, 1815, 2411, 3125, 3415, 3444
ApaI	GGGCCC	1 cut(s)	3129
ApeKI	GCWGC	5 cut(s)	1463, 3002, 3102, 3536, 3544
ApoI	RAATTY	4 cut(s)	2281, 2684, 3111, 3191
AseI	ATTAAT	1 cut(s)	918
Asp700I	GAANNNNTTC	4 cut(s)	693, 1182, 1274, 1699
Asp718I	GGTACC	2 cut(s)	249, 3608
AspLEI	GCGC	5 cut(s)	90, 184, 316, 1713, 3102
AspS9I	GGNCC	9 cut(s)	234, 907, 1830, 2161, 2475, 2701, 2710, 3125, 3126
AsuC2I	CCSGG	4 cut(s)	1766, 1767, 3667, 3668
AsuHPI	GGTGA	9 cut(s)	179, 627, 706, 2024, 2921, 2922, 3328, 3682, 3692
AsuNHI	GCTAGC	1 cut(s)	800
AvaI	CYCGRG	2 cut(s)	1765, 3666
AvaII	GGWCC	5 cut(s)	1830, 2161, 2475, 2701, 2710
BaeGI	GKGCMC	1 cut(s)	3129
BaeI	ACNNNNGTAYC	4 cut(s)	980, 1013, 1334, 1367
BalI	TGGCCA	4 cut(s)	197, 1817, 2413, 3417
BanI	GGYRCC	2 cut(s)	249, 3608
BanII	GRGCYC	2 cut(s)	177, 3129
BarI	GAAGNNNNNNTAC	2 cut(s)	3127, 3159
BauI	CACGAG	1 cut(s)	2199
BbsI	GAAGAC	4 cut(s)	300, 1536, 2196, 2892
Bbv12I	GWGCWC	4 cut(s)	177, 1238, 2077, 2496
BbvCI	CCTCAGC	2 cut(s)	2088, 2487
BbvI	GCAGC	5 cut(s)	1475, 2989, 3089, 3523, 3556
BccI	CCATC	7 cut(s)	592, 715, 918, 1100, 1500, 1864, 2548
BceAI	ACGGC	1 cut(s)	1743
BciT130I	CCWGG	6 cut(s)	718, 1882, 2534, 2727, 3302, 3332
BclI	TGATCA	5 cut(s)	385, 1246, 1732, 1747, 3672
BcnI	CCSGG	4 cut(s)	1766, 1767, 3667, 3668
BcoDI	GTCTC	3 cut(s)	1642, 2642, 3492
BfmI	CTRYAG	1 cut(s)	1098
BfoI	RGCGCY	2 cut(s)	91, 317
BfuAI	ACCTGC	1 cut(s)	1285
BglI	GCCNNNNNGGC	3 cut(s)	977, 3124, 3533
BisI	GCNGC	9 cut(s)	1464, 1709, 2292, 2851, 3003, 3103, 3244, 3537, 3545
BlpI	GCTNAGC	1 cut(s)	3540
BlsI	GCNGC	9 cut(s)	1465, 1710, 2293, 2852, 3004, 3104, 3245, 3538, 3546
BmcAI	AGTACT	1 cut(s)	883
Bme18I	GGWCC	5 cut(s)	1830, 2161, 2475, 2701, 2710
BmeT110I	CYCGRG	2 cut(s)	1765, 3666
BmgT120I	GGNCC	9 cut(s)	234, 907, 1830, 2161, 2475, 2701, 2710, 3125, 3126
BmiI	GGNNCC	6 cut(s)	251, 463, 908, 2476, 3127, 3610
BmrI	ACTGGG	2 cut(s)	864, 912
BmsI	GCATC	6 cut(s)	169, 458, 563, 1359, 2785, 3514
BmtI	GCTAGC	1 cut(s)	804
BmuI	ACTGGG	2 cut(s)	864, 912
BpiI	GAAGAC	4 cut(s)	300, 1536, 2196, 2892
BplI	GAGNNNNNCTC	2 cut(s)	1220, 1252
BpmI	CTGGAG	4 cut(s)	263, 1020, 1452, 3353
Bpu10I	CCTNAGC	2 cut(s)	2088, 2487
Bpu1102I	GCTNAGC	1 cut(s)	3540
BpuEI	CTTGAG	3 cut(s)	280, 2423, 2865
BpuMI	CCSGG	4 cut(s)	1766, 1767, 3667, 3668
Bsa29I	ATCGAT	1 cut(s)	266
BsaAI	YACGTR	1 cut(s)	326
BsaBI	GATNNNNATC	2 cut(s)	645, 3597
BsaHI	GRCGYC	1 cut(s)	8
BsaXI	ACNNNNNCTCC	2 cut(s)	110, 140
Bse1I	ACTGG	9 cut(s)	859, 907, 1469, 1656, 1869, 2163, 3119, 3387, 3629
Bse3DI	GCAATG	4 cut(s)	1288, 3157, 3220, 3277
Bse8I	GATNNNNATC	2 cut(s)	645, 3597
BseBI	CCWGG	6 cut(s)	718, 1882, 2534, 2727, 3302, 3332
BseCI	ATCGAT	1 cut(s)	266
BseGI	GGATG	9 cut(s)	726, 910, 1511, 2098, 2260, 2355, 2542, 3383, 3579
BseJI	GATNNNNATC	2 cut(s)	645, 3597
BseMI	GCAATG	4 cut(s)	1288, 3157, 3220, 3277
BseMII	CTCAG	6 cut(s)	785, 2079, 2478, 2637, 2879, 3531
BseNI	ACTGG	9 cut(s)	859, 907, 1469, 1656, 1869, 2163, 3119, 3387, 3629
BseRI	GAGGAG	8 cut(s)	128, 131, 134, 515, 560, 759, 989, 2456
BseSI	GKGCMC	1 cut(s)	3129
BseXI	GCAGC	5 cut(s)	1475, 2989, 3089, 3523, 3556
BsgI	GTGCAG	3 cut(s)	1212, 1313, 2508
Bsh1236I	CGCG	1 cut(s)	1711
BshFI	GGCC	9 cut(s)	197, 235, 602, 909, 1817, 2413, 3127, 3417, 3446
BshNI	GGYRCC	2 cut(s)	249, 3608
BshVI	ATCGAT	1 cut(s)	266
BsiHKAI	GWGCWC	4 cut(s)	177, 1238, 2077, 2496
BsiHKCI	CYCGRG	2 cut(s)	1765, 3666
BsiSI	CCGG	2 cut(s)	1766, 3667
BslFI	GGGAC	3 cut(s)	1457, 1748, 2488
BsmAI	GTCTC	3 cut(s)	1642, 2642, 3492
BsmFI	GGGAC	3 cut(s)	1457, 1748, 2488
BsmI	GAATGC	2 cut(s)	2615, 3427
BsnI	GGCC	9 cut(s)	197, 235, 602, 909, 1817, 2413, 3127, 3417, 3446
BsoBI	CYCGRG	2 cut(s)	1765, 3666
Bsp120I	GGGCCC	1 cut(s)	3125
Bsp1286I	GDGCHC	5 cut(s)	177, 1238, 2077, 2496, 3129
Bsp1407I	TGTACA	1 cut(s)	455
Bsp1720I	GCTNAGC	1 cut(s)	3540
Bsp19I	CCATGG	2 cut(s)	198, 1400
BspANI	GGCC	9 cut(s)	197, 235, 602, 909, 1817, 2413, 3127, 3417, 3446
BspCNI	CTCAG	6 cut(s)	784, 2080, 2479, 2638, 2878, 3532
BspDI	ATCGAT	1 cut(s)	266
BspFNI	CGCG	1 cut(s)	1711
BspHI	TCATGA	2 cut(s)	760, 2227
BspLI	GGNNCC	6 cut(s)	251, 463, 908, 2476, 3127, 3610
BspMAI	CTGCAG	1 cut(s)	1102
BspMI	ACCTGC	1 cut(s)	1285
BspOI	GCTAGC	1 cut(s)	804
BspPI	GGATC	4 cut(s)	1804, 2629, 2801, 3661
BspQI	GCTCTTC	1 cut(s)	1852
BspT107I	GGYRCC	2 cut(s)	249, 3608
BsrBI	CCGCTC	1 cut(s)	247
BsrDI	GCAATG	4 cut(s)	1288, 3157, 3220, 3277
BsrGI	TGTACA	1 cut(s)	455
BsrI	ACTGG	9 cut(s)	859, 907, 1469, 1656, 1869, 2163, 3119, 3387, 3629
BssNI	GRCGYC	1 cut(s)	8
BssSI	CACGAG	1 cut(s)	2199
BssT1I	CCWWGG	5 cut(s)	198, 1400, 2510, 2810, 3247
Bst2BI	CACGAG	1 cut(s)	2199
Bst2UI	CCWGG	6 cut(s)	718, 1882, 2534, 2727, 3302, 3332
Bst6I	CTCTTC	1 cut(s)	1852
BstACI	GRCGYC	1 cut(s)	8
BstAPI	GCANNNNNTGC	5 cut(s)	3102, 3186, 3240, 3288, 3297
BstAUI	TGTACA	1 cut(s)	455
BstBAI	YACGTR	1 cut(s)	326
BstDEI	CTNAG	7 cut(s)	404, 771, 2088, 2487, 2646, 2865, 3540
BstDSI	CCRYGG	4 cut(s)	198, 614, 1400, 2478
BstENI	CCTNNNNNAGG	1 cut(s)	3251
BstF5I	GGATG	9 cut(s)	726, 910, 1511, 2098, 2260, 2355, 2542, 3383, 3579
BstFNI	CGCG	1 cut(s)	1711
BstH2I	RGCGCY	2 cut(s)	91, 317
BstHHI	GCGC	5 cut(s)	90, 184, 316, 1713, 3102
BstMAI	GTCTC	3 cut(s)	1642, 2642, 3492
BstNI	CCWGG	6 cut(s)	718, 1882, 2534, 2727, 3302, 3332
BstNSI	RCATGY	9 cut(s)	1136, 1456, 1515, 1617, 3030, 3100, 3291, 3427, 3520
BstSFI	CTRYAG	1 cut(s)	1098
BstSLI	GKGCMC	1 cut(s)	3129
BstUI	CGCG	1 cut(s)	1711
BstV1I	GCAGC	5 cut(s)	1475, 2989, 3089, 3523, 3556
BstV2I	GAAGAC	4 cut(s)	300, 1536, 2196, 2892
BstX2I	RGATCY	1 cut(s)	2621
BstYI	RGATCY	1 cut(s)	2621
Bsu15I	ATCGAT	1 cut(s)	266
BsuRI	GGCC	9 cut(s)	197, 235, 602, 909, 1817, 2413, 3127, 3417, 3446
BsuTUI	ATCGAT	1 cut(s)	266
BtgI	CCRYGG	4 cut(s)	198, 614, 1400, 2478
BtsCI	GGATG	9 cut(s)	726, 910, 1511, 2098, 2260, 2355, 2542, 3383, 3579
BtsIMutI	CAGTG	7 cut(s)	900, 1710, 1726, 2156, 2433, 3394, 3622
BveI	ACCTGC	1 cut(s)	1285
CaiI	CAGNNNCTG	1 cut(s)	1475
CciI	TCATGA	2 cut(s)	760, 2227
CfoI	GCGC	5 cut(s)	90, 184, 316, 1713, 3102
Cfr13I	GGNCC	9 cut(s)	234, 907, 1830, 2161, 2475, 2701, 2710, 3125, 3126
Cfr9I	CCCGGG	2 cut(s)	1765, 3666
ClaI	ATCGAT	1 cut(s)	266
CseI	GACGC	2 cut(s)	16, 2934
Csp6I	GTAC	8 cut(s)	250, 456, 732, 882, 1351, 1743, 3135, 3609
CviQI	GTAC	8 cut(s)	250, 456, 732, 882, 1351, 1743, 3135, 3609
DdeI	CTNAG	7 cut(s)	404, 771, 2088, 2487, 2646, 2865, 3540
DraIII	CACNNNGTG	1 cut(s)	422
DrdI	GACNNNNNNGTC	2 cut(s)	100, 2456
DseDI	GACNNNNNNGTC	2 cut(s)	100, 2456
EaeI	YGGCCR	5 cut(s)	195, 600, 1815, 2411, 3415
Eam1104I	CTCTTC	1 cut(s)	1852
EarI	CTCTTC	1 cut(s)	1852
EciI	GGCGGA	2 cut(s)	45, 2479
Ecl136II	GAGCTC	1 cut(s)	175
Eco130I	CCWWGG	5 cut(s)	198, 1400, 2510, 2810, 3247
Eco147I	AGGCCT	1 cut(s)	3446
Eco24I	GRGCYC	2 cut(s)	177, 3129
Eco32I	GATATC	2 cut(s)	229, 1116
Eco47I	GGWCC	5 cut(s)	1830, 2161, 2475, 2701, 2710
Eco47III	AGCGCT	1 cut(s)	315
Eco53kI	GAGCTC	1 cut(s)	175
Eco57I	CTGAAG	7 cut(s)	1071, 1158, 1194, 1261, 1306, 1443, 2610
Eco88I	CYCGRG	2 cut(s)	1765, 3666
EcoICRI	GAGCTC	1 cut(s)	175
EcoNI	CCTNNNNNAGG	1 cut(s)	3251
EcoO109I	RGGNCCY	1 cut(s)	234
EcoRII	CCWGG	6 cut(s)	716, 1880, 2532, 2725, 3300, 3330
EcoRV	GATATC	2 cut(s)	229, 1116
EcoT14I	CCWWGG	5 cut(s)	198, 1400, 2510, 2810, 3247
EcoT22I	ATGCAT	3 cut(s)	1367, 2615, 3429
EcoT38I	GRGCYC	2 cut(s)	177, 3129
ErhI	CCWWGG	5 cut(s)	198, 1400, 2510, 2810, 3247
FalI	AAGNNNNNCTT	8 cut(s)	74, 106, 1103, 1135, 2010, 2042, 2115, 2147
FaqI	GGGAC	3 cut(s)	1457, 1748, 2488
FauI	CCCGC	2 cut(s)	1332, 1618
FauNDI	CATATG	2 cut(s)	2250, 3131
FbaI	TGATCA	5 cut(s)	385, 1246, 1732, 1747, 3672
FblI	GTMKAC	1 cut(s)	814
Fnu4HI	GCNGC	9 cut(s)	1464, 1709, 2292, 2851, 3003, 3103, 3244, 3537, 3545
FokI	GGATG	9 cut(s)	733, 897, 1518, 2105, 2267, 2362, 2549, 3370, 3566
FriOI	GRGCYC	2 cut(s)	177, 3129
Fsp4HI	GCNGC	9 cut(s)	1464, 1709, 2292, 2851, 3003, 3103, 3244, 3537, 3545
GlaI	GCGC	5 cut(s)	89, 183, 315, 1712, 3101
GluI	GCNGC	9 cut(s)	1464, 1709, 2292, 2851, 3003, 3103, 3244, 3537, 3545
GsuI	CTGGAG	4 cut(s)	263, 1020, 1452, 3353
HaeII	RGCGCY	2 cut(s)	91, 317
HaeIII	GGCC	9 cut(s)	197, 235, 602, 909, 1817, 2413, 3127, 3417, 3446
HapII	CCGG	2 cut(s)	1766, 3667
HgaI	GACGC	2 cut(s)	16, 2934
HhaI	GCGC	5 cut(s)	90, 184, 316, 1713, 3102
Hin1I	GRCGYC	1 cut(s)	8
Hin6I	GCGC	5 cut(s)	88, 182, 314, 1711, 3100
HinP1I	GCGC	5 cut(s)	88, 182, 314, 1711, 3100
HincII	GTYRAC	4 cut(s)	15, 61, 1419, 3268
HindII	GTYRAC	4 cut(s)	15, 61, 1419, 3268
HindIII	AAGCTT	3 cut(s)	692, 2339, 2695
HpaI	GTTAAC	1 cut(s)	15
HpaII	CCGG	2 cut(s)	1766, 3667
HphI	GGTGA	9 cut(s)	179, 627, 706, 2024, 2921, 2922, 3328, 3682, 3692
Hpy99I	CGWCG	4 cut(s)	10, 106, 109, 112
HpyAV	CCTTC	7 cut(s)	163, 719, 2234, 2728, 2943, 3153, 3247
HpyCH4IV	ACGT	1 cut(s)	325
HpyF3I	CTNAG	7 cut(s)	404, 771, 2088, 2487, 2646, 2865, 3540
HpySE526I	ACGT	1 cut(s)	325
Hsp92I	GRCGYC	1 cut(s)	8
HspAI	GCGC	5 cut(s)	88, 182, 314, 1711, 3100
KpnI	GGTACC	2 cut(s)	253, 3612
Ksp22I	TGATCA	5 cut(s)	385, 1246, 1732, 1747, 3672
KspAI	GTTAAC	1 cut(s)	15
LguI	GCTCTTC	1 cut(s)	1852
LmnI	GCTCC	9 cut(s)	59, 172, 244, 461, 547, 1196, 1471, 2443, 2499
Lsp1109I	GCAGC	5 cut(s)	1475, 2989, 3089, 3523, 3556
LweI	GCATC	6 cut(s)	169, 458, 563, 1359, 2785, 3514
MaeII	ACGT	1 cut(s)	325
MaeIII	GTNAC	8 cut(s)	1489, 2368, 2505, 2515, 2660, 2874, 3458, 3644
MbiI	CCGCTC	1 cut(s)	247
MfeI	CAATTG	1 cut(s)	3292
MflI	RGATCY	1 cut(s)	2621
MhlI	GDGCHC	5 cut(s)	177, 1238, 2077, 2496, 3129
MlsI	TGGCCA	4 cut(s)	197, 1817, 2413, 3417
MluNI	TGGCCA	4 cut(s)	197, 1817, 2413, 3417
MlyI	GAGTC	5 cut(s)	884, 2239, 2777, 2794, 2906
MmeI	TCCRAC	2 cut(s)	90, 2711
Mox20I	TGGCCA	4 cut(s)	197, 1817, 2413, 3417
Mph1103I	ATGCAT	3 cut(s)	1367, 2615, 3429
MroXI	GAANNNNTTC	4 cut(s)	693, 1182, 1274, 1699
MscI	TGGCCA	4 cut(s)	197, 1817, 2413, 3417
MslI	CAYNNNNRTG	7 cut(s)	285, 719, 844, 2082, 2253, 3045, 3145
Msp20I	TGGCCA	4 cut(s)	197, 1817, 2413, 3417
MspA1I	CMGCKG	4 cut(s)	213, 1214, 1339, 2853
MspI	CCGG	2 cut(s)	1766, 3667
MunI	CAATTG	1 cut(s)	3292
Mva1269I	GAATGC	2 cut(s)	2615, 3427
MvaI	CCWGG	6 cut(s)	718, 1882, 2534, 2727, 3302, 3332
MvnI	CGCG	1 cut(s)	1711
NciI	CCSGG	4 cut(s)	1766, 1767, 3667, 3668
NcoI	CCATGG	2 cut(s)	198, 1400
NdeI	CATATG	2 cut(s)	2250, 3131
NheI	GCTAGC	1 cut(s)	800
NlaIV	GGNNCC	6 cut(s)	251, 463, 908, 2476, 3127, 3610
NmeAIII	GCCGAG	3 cut(s)	628, 997, 1102
NmuCI	GTSAC	2 cut(s)	2368, 3458
NsiI	ATGCAT	3 cut(s)	1367, 2615, 3429
NspI	RCATGY	9 cut(s)	1136, 1456, 1515, 1617, 3030, 3100, 3291, 3427, 3520
PaeI	GCATGC	4 cut(s)	1136, 1515, 3100, 3520
PagI	TCATGA	2 cut(s)	760, 2227
PceI	AGGCCT	1 cut(s)	3446
PciSI	GCTCTTC	1 cut(s)	1852
PctI	GAATGC	2 cut(s)	2615, 3427
PdmI	GAANNNNTTC	4 cut(s)	693, 1182, 1274, 1699
PflFI	GACNNNGTC	1 cut(s)	1085
PflMI	CCANNNNNTGG	3 cut(s)	490, 2420, 3064
PkrI	GCNGC	9 cut(s)	1465, 1710, 2293, 2852, 3004, 3104, 3245, 3538, 3546
PleI	GAGTC	5 cut(s)	883, 2239, 2776, 2794, 2905
PpsI	GAGTC	5 cut(s)	883, 2239, 2776, 2794, 2905
Ppu21I	YACGTR	1 cut(s)	326
PshBI	ATTAAT	1 cut(s)	918
Psp124BI	GAGCTC	1 cut(s)	177
Psp6I	CCWGG	6 cut(s)	716, 1880, 2532, 2725, 3300, 3330
PspGI	CCWGG	6 cut(s)	716, 1880, 2532, 2725, 3300, 3330
PspN4I	GGNNCC	6 cut(s)	251, 463, 908, 2476, 3127, 3610
PspOMI	GGGCCC	1 cut(s)	3125
PspPI	GGNCC	9 cut(s)	234, 907, 1830, 2161, 2475, 2701, 2710, 3125, 3126
PsrI	GAACNNNNNNTAC	4 cut(s)	498, 530, 1516, 1548
PstI	CTGCAG	1 cut(s)	1102
PstNI	CAGNNNCTG	1 cut(s)	1475
PsuI	RGATCY	1 cut(s)	2621
PsyI	GACNNNGTC	1 cut(s)	1085
PvuII	CAGCTG	1 cut(s)	1214
RsaI	GTAC	8 cut(s)	251, 457, 733, 883, 1352, 1744, 3136, 3610
RsaNI	GTAC	8 cut(s)	250, 456, 732, 882, 1351, 1743, 3135, 3609
RseI	CAYNNNNRTG	7 cut(s)	285, 719, 844, 2082, 2253, 3045, 3145
SacI	GAGCTC	1 cut(s)	177
SapI	GCTCTTC	1 cut(s)	1852
SatI	GCNGC	9 cut(s)	1464, 1709, 2292, 2851, 3003, 3103, 3244, 3537, 3545
Sau96I	GGNCC	9 cut(s)	234, 907, 1830, 2161, 2475, 2701, 2710, 3125, 3126
ScaI	AGTACT	1 cut(s)	883
SchI	GAGTC	5 cut(s)	884, 2239, 2777, 2794, 2906
SduI	GDGCHC	5 cut(s)	177, 1238, 2077, 2496, 3129
SfaNI	GCATC	6 cut(s)	169, 458, 563, 1359, 2785, 3514
SfcI	CTRYAG	1 cut(s)	1098
SinI	GGWCC	5 cut(s)	1830, 2161, 2475, 2701, 2710
SmaI	CCCGGG	2 cut(s)	1767, 3668
SmiMI	CAYNNNNRTG	7 cut(s)	285, 719, 844, 2082, 2253, 3045, 3145
SmlI	CTYRAG	3 cut(s)	259, 2402, 2880
SmoI	CTYRAG	3 cut(s)	259, 2402, 2880
SphI	GCATGC	4 cut(s)	1136, 1515, 3100, 3520
SseBI	AGGCCT	1 cut(s)	3446
SspI	AATATT	1 cut(s)	1441
SstI	GAGCTC	1 cut(s)	177
StuI	AGGCCT	1 cut(s)	3446
StyI	CCWWGG	5 cut(s)	198, 1400, 2510, 2810, 3247
TaiI	ACGT	1 cut(s)	328
TaqI	TCGA	8 cut(s)	69, 177, 266, 1312, 1386, 1606, 1799, 3601
TatI	WGTACW	4 cut(s)	455, 881, 1742, 3134
TauI	GCSGC	4 cut(s)	1711, 2294, 2853, 3246
TscAI	CASTG	7 cut(s)	907, 1710, 1726, 2163, 2440, 3394, 3629
TseFI	GTSAC	2 cut(s)	2368, 3458
TseI	GCWGC	5 cut(s)	1463, 3002, 3102, 3536, 3544
Tsp45I	GTSAC	2 cut(s)	2368, 3458
TspGWI	ACGGA	7 cut(s)	39, 46, 239, 603, 2039, 2723, 2735
TspMI	CCCGGG	2 cut(s)	1765, 3666
TspRI	CASTG	7 cut(s)	907, 1710, 1726, 2163, 2440, 3394, 3629
Tth111I	GACNNNGTC	1 cut(s)	1085
Van91I	CCANNNNNTGG	3 cut(s)	490, 2420, 3064
VpaK11BI	GGWCC	5 cut(s)	1830, 2161, 2475, 2701, 2710
VspI	ATTAAT	1 cut(s)	918
XagI	CCTNNNNNAGG	1 cut(s)	3251
XapI	RAATTY	4 cut(s)	2281, 2684, 3111, 3191
XceI	RCATGY	9 cut(s)	1136, 1456, 1515, 1617, 3030, 3100, 3291, 3427, 3520
XmaI	CCCGGG	2 cut(s)	1765, 3666
XmiI	GTMKAC	1 cut(s)	814
XmnI	GAANNNNTTC	4 cut(s)	693, 1182, 1274, 1699
ZrmI	AGTACT	1 cut(s)	883
Zsp2I	ATGCAT	3 cut(s)	1367, 2615, 3429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.