MD07G1097200.v1.1

Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003640
Physical Location & Seq
Forward (+)
10677110 .. 10680471
3362 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1097200.v1.1.491

Sequence Viewer

Length: 1236 bp
ATGTGGAACAGAACAGACCAGAACAAAAATAAGAGAGGTTATAAAGGTTCCATACGTAAACGAGTCACCGACCAAACGGTTGTAACTCTACTTTTCATCCGTCGTTTTTCACTCCCCGTCTTGTTCGTGCTCTCAAGCCTTGCCAACATCATCAGATTTTCCAAAACCAACAACACGAACCCAACGAATTATCTCAAAAACAATTTCCGGGTCTTTCATTTCAGTCCAAAATCTCAGATGGGTCGTCGGCAGAGCGACTCAGAACTCGGCCGTCTCACACTTCTGGCCCTTTTCCTGATGGGCGCAATCTTCTGCTGCATGGTGTACCTGTTCCTCGCTGTGGCCTCAAAACCCAGCAGCATAACAACAGCTGATTCGGTTTCGGAATTCGGAGACGTTGGATGGAGGAGTGGAGAAGAAGATGGAGAATGCTGCAGAGGGATTGAGAATTTAGAGCTCTGGGGTGATGCTGTGAAATGGGGTTCTGAGTTTAAGGTGAATTCTTCTAAAGAGTGCTGCATGTCTTGTAAGGATATGTGCGGGGATCGAGATGGGCATTGTTTGTGTGACAGTTGGGTGTTTTGTGGGGATAGAGAGGCTTGTGGACTTAGATATGGTGAGTGTTGGTTAAAGAAACAAAAGGATACCTTGGACCCTGATCGGAAAGACTCAGGTGATCTGATTGCATGGACTTCTGGACTCGTCTTTGGAAAAGGAGAGGGTATTGTCGGCTTGGAAACAGAGTATGGGACTCTTCATATAAAGCTCCGTCCTGATTGTGCCCCACATTCTGTTGCCTACATTCTTGAGCTGTTGCAAGTGCCCCGTTATGCAGGCTGCCAATTTTATCGCGCAGAAAACCGGGGAAGCTTTTGGGACTCACTAGGAAACCATGTAAAAAATGCTCCGTTTGGCCCGCCTTTCGCGCTAATACAAGGAACGCTTGCAGCTCACGGGTTCGTATTTAAGGAGATTCCGGCAGAGGTGTCTGGCACCATCAGAAGAGGATCGGTTGCCTGGATTGGTTCCGGTCCAGAATTCTTCATCAGCCTAGCCAACCATTTTGAGTGGAAAAAAGCTTACACTGTGTTTGGTTCCGTTCTTCCTGAAGACATGGAAATCGCCGAGAAAATTGCCCAGCTTCCGACCAAACAAGAGGTCTGGAGTAACATCAATGTCTCCGTCTTGGAGAGTCCTGTCGCTTTATGGTTCCGAAGAATCAAGCCGGAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAN_4 Pro_isomerase

Protein Analysis

412

Amino Acids

46.19

Weight (kDa)

8.29

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 243 - 396 6.1e-13 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 42
AccB1I GGYRCC 1 cut(s) 992
AccII CGCG 2 cut(s) 852, 926
AciI CCGC 2 cut(s) 540, 917
AclWI GGATC 2 cut(s) 552, 1015
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 4 cut(s) 386, 448, 499, 1037
AcuI CTGAAG 1 cut(s) 1128
AfaI GTAC 1 cut(s) 326
AfiI CCNNNNNNNGG 1 cut(s) 340
AjnI CCWGG 1 cut(s) 1016
AluBI AGCT 8 cut(s) 371, 457, 766, 811, 870, 950, 1079, 1141
AluI AGCT 8 cut(s) 371, 457, 766, 811, 870, 950, 1079, 1141
Alw21I GWGCWC 2 cut(s) 132, 459
Alw26I GTCTC 3 cut(s) 278, 387, 1183
AlwI GGATC 2 cut(s) 552, 1015
AoxI GGCC 4 cut(s) 268, 285, 342, 913
ApeKI GCWGC 6 cut(s) 315, 357, 432, 516, 837, 947
ApoI RAATTY 4 cut(s) 386, 448, 499, 1037
ArsI GACNNNNNNTTYG 2 cut(s) 1143, 1175
AspLEI GCGC 3 cut(s) 305, 854, 928
AspS9I GGNCC 4 cut(s) 286, 652, 914, 1031
AsuC2I CCSGG 2 cut(s) 209, 863
AsuHPI GGTGA 5 cut(s) 58, 476, 508, 629, 686
AvaII GGWCC 2 cut(s) 652, 1031
BaeGI GKGCMC 2 cut(s) 784, 825
BanI GGYRCC 1 cut(s) 992
BanII GRGCYC 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 1116
Bbv12I GWGCWC 2 cut(s) 132, 459
BbvI GCAGC 6 cut(s) 302, 369, 419, 503, 824, 959
BccI CCATC 6 cut(s) 232, 292, 396, 416, 545, 1004
BceAI ACGGC 1 cut(s) 255
BcgI CGANNNNNNTGC 2 cut(s) 1115, 1149
BciT130I CCWGG 1 cut(s) 1018
BciVI GTATCC 1 cut(s) 637
BcnI CCSGG 2 cut(s) 209, 863
BcoDI GTCTC 3 cut(s) 278, 387, 1183
BfaI CTAG 2 cut(s) 884, 1052
BfmI CTRYAG 1 cut(s) 433
BfuI GTATCC 1 cut(s) 637
BisI GCNGC 6 cut(s) 316, 358, 433, 517, 838, 948
BlsI GCNGC 6 cut(s) 317, 359, 434, 518, 839, 949
Bme1390I CCNGG 3 cut(s) 209, 863, 1018
Bme18I GGWCC 2 cut(s) 652, 1031
BmgT120I GGNCC 4 cut(s) 286, 652, 914, 1031
BmiI GGNNCC 6 cut(s) 49, 654, 994, 1027, 1096, 1211
BmrFI CCNGG 3 cut(s) 209, 863, 1018
BmsI GCATC 1 cut(s) 457
BpiI GAAGAC 1 cut(s) 1116
BpmI CTGGAG 1 cut(s) 1183
BpuEI CTTGAG 2 cut(s) 118, 827
BpuMI CCSGG 2 cut(s) 209, 863
BsaAI YACGTR 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 648, 862
BsaWI WCCGGW 1 cut(s) 1028
Bsc4I CCNNNNNNNGG 1 cut(s) 340
BseBI CCWGG 1 cut(s) 1018
BseDI CCNNGG 2 cut(s) 648, 862
BseGI GGATG 2 cut(s) 96, 407
BseLI CCNNNNNNNGG 1 cut(s) 340
BseMII CTCAG 4 cut(s) 248, 273, 477, 684
BseRI GAGGAG 1 cut(s) 421
BseSI GKGCMC 2 cut(s) 784, 825
BseX3I CGGCCG 1 cut(s) 268
BseXI GCAGC 6 cut(s) 302, 369, 419, 503, 824, 959
BseYI CCCAGC 2 cut(s) 353, 1137
Bsh1236I CGCG 2 cut(s) 852, 926
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 4 cut(s) 270, 287, 344, 915
BshNI GGYRCC 1 cut(s) 992
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 2 cut(s) 132, 459
BsiSI CCGG 5 cut(s) 208, 862, 977, 1029, 1226
BslFI GGGAC 2 cut(s) 763, 890
BslI CCNNNNNNNGG 1 cut(s) 340
BsmAI GTCTC 3 cut(s) 278, 387, 1183
BsmBI CGTCTC 2 cut(s) 278, 387
BsmFI GGGAC 2 cut(s) 763, 890
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 4 cut(s) 270, 287, 344, 915
Bsp1286I GDGCHC 4 cut(s) 132, 459, 784, 825
Bsp143I GATC 4 cut(s) 544, 658, 676, 1007
BspACI CCGC 2 cut(s) 540, 917
BspANI GGCC 4 cut(s) 270, 287, 344, 915
BspCNI CTCAG 4 cut(s) 247, 272, 478, 683
BspFNI CGCG 2 cut(s) 852, 926
BspHI TCATGA 1 cut(s) 1232
BspLI GGNNCC 6 cut(s) 49, 654, 994, 1027, 1096, 1211
BspMAI CTGCAG 1 cut(s) 437
BspPI GGATC 2 cut(s) 552, 1015
BspT107I GGYRCC 1 cut(s) 992
BssECI CCNNGG 2 cut(s) 648, 862
BssMI GATC 4 cut(s) 544, 658, 676, 1007
BssT1I CCWWGG 1 cut(s) 648
Bst2UI CCWGG 1 cut(s) 1018
Bst4CI ACNGT 3 cut(s) 79, 572, 1087
Bst6I CTCTTC 2 cut(s) 759, 997
BstBAI YACGTR 1 cut(s) 56
BstC8I GCNNGC 3 cut(s) 835, 917, 945
BstDEI CTNAG 5 cut(s) 234, 259, 486, 608, 670
BstF5I GGATG 2 cut(s) 96, 407
BstFNI CGCG 2 cut(s) 852, 926
BstHHI GCGC 3 cut(s) 305, 854, 928
BstKTI GATC 4 cut(s) 547, 661, 679, 1010
BstMAI GTCTC 3 cut(s) 278, 387, 1183
BstMBI GATC 4 cut(s) 544, 658, 676, 1007
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 1 cut(s) 925
BstNI CCWGG 1 cut(s) 1018
BstNSI RCATGY 1 cut(s) 523
BstSCI CCNGG 3 cut(s) 207, 861, 1016
BstSFI CTRYAG 1 cut(s) 433
BstSLI GKGCMC 2 cut(s) 784, 825
BstSNI TACGTA 1 cut(s) 56
BstUI CGCG 2 cut(s) 852, 926
BstV1I GCAGC 6 cut(s) 302, 369, 419, 503, 824, 959
BstV2I GAAGAC 1 cut(s) 1116
BstZI CGGCCG 1 cut(s) 268
BsuI GTATCC 1 cut(s) 637
BsuRI GGCC 4 cut(s) 270, 287, 344, 915
BtsCI GGATG 2 cut(s) 96, 407
BtsIMutI CAGTG 1 cut(s) 1083
Cac8I GCNNGC 3 cut(s) 835, 917, 945
CciI TCATGA 1 cut(s) 1232
CfoI GCGC 3 cut(s) 305, 854, 928
Cfr13I GGNCC 4 cut(s) 286, 652, 914, 1031
Csp6I GTAC 1 cut(s) 325
CviAII CATG 6 cut(s) 319, 520, 687, 893, 1114, 1233
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 5 cut(s) 234, 259, 486, 608, 670
DpnI GATC 4 cut(s) 546, 660, 678, 1009
DpnII GATC 4 cut(s) 544, 658, 676, 1007
EaeI YGGCCR 1 cut(s) 268
EagI CGGCCG 1 cut(s) 268
Eam1104I CTCTTC 2 cut(s) 759, 997
EarI CTCTTC 2 cut(s) 759, 997
Ecl136II GAGCTC 1 cut(s) 457
EclXI CGGCCG 1 cut(s) 268
Eco105I TACGTA 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 648
Eco24I GRGCYC 1 cut(s) 459
Eco47I GGWCC 2 cut(s) 652, 1031
Eco52I CGGCCG 1 cut(s) 268
Eco53kI GAGCTC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 1128
EcoICRI GAGCTC 1 cut(s) 457
EcoRI GAATTC 3 cut(s) 386, 499, 1037
EcoRII CCWGG 1 cut(s) 1016
EcoT14I CCWWGG 1 cut(s) 648
EcoT38I GRGCYC 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 648
Esp3I CGTCTC 2 cut(s) 278, 387
FaeI CATG 6 cut(s) 322, 523, 690, 896, 1117, 1236
FalI AAGNNNNNCTT 4 cut(s) 632, 664, 927, 959
FaqI GGGAC 2 cut(s) 763, 890
FatI CATG 6 cut(s) 318, 519, 686, 892, 1113, 1232
FauI CCCGC 2 cut(s) 533, 924
Fnu4HI GCNGC 6 cut(s) 316, 358, 433, 517, 838, 948
FokI GGATG 2 cut(s) 83, 414
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 6 cut(s) 316, 358, 433, 517, 838, 948
FspBI CTAG 2 cut(s) 884, 1052
GlaI GCGC 3 cut(s) 304, 853, 927
GluI GCNGC 6 cut(s) 316, 358, 433, 517, 838, 948
GsaI CCCAGC 2 cut(s) 357, 1141
GsuI CTGGAG 1 cut(s) 1183
HaeIII GGCC 4 cut(s) 270, 287, 344, 915
HapII CCGG 5 cut(s) 208, 862, 977, 1029, 1226
HhaI GCGC 3 cut(s) 305, 854, 928
Hin1II CATG 6 cut(s) 322, 523, 690, 896, 1117, 1236
Hin6I GCGC 3 cut(s) 303, 852, 926
HinP1I GCGC 3 cut(s) 303, 852, 926
HindIII AAGCTT 2 cut(s) 868, 1077
HpaII CCGG 5 cut(s) 208, 862, 977, 1029, 1226
HphI GGTGA 5 cut(s) 58, 476, 508, 629, 686
Hpy166II GTNNAC 3 cut(s) 59, 325, 605
Hpy188III TCNNGA 9 cut(s) 295, 548, 696, 773, 806, 1034, 1106, 1162, 1233
Hpy8I GTNNAC 3 cut(s) 59, 325, 605
Hpy99I CGWCG 2 cut(s) 105, 249
HpyCH4III ACNGT 3 cut(s) 79, 572, 1087
HpyCH4IV ACGT 2 cut(s) 55, 396
HpyCH4V TGCA 7 cut(s) 318, 435, 519, 686, 817, 833, 947
HpyF10VI GCNNNNNNNGC 1 cut(s) 925
HpyF3I CTNAG 5 cut(s) 234, 259, 486, 608, 670
HpySE526I ACGT 2 cut(s) 55, 396
Hsp92II CATG 6 cut(s) 322, 523, 690, 896, 1117, 1236
HspAI GCGC 3 cut(s) 303, 852, 926
Kzo9I GATC 4 cut(s) 544, 658, 676, 1007
LmnI GCTCC 2 cut(s) 771, 910
Lsp1109I GCAGC 6 cut(s) 302, 369, 419, 503, 824, 959
LweI GCATC 1 cut(s) 457
MaeI CTAG 2 cut(s) 884, 1052
MaeII ACGT 2 cut(s) 55, 396
MaeIII GTNAC 4 cut(s) 64, 82, 566, 1166
MalI GATC 4 cut(s) 546, 660, 678, 1009
MboI GATC 4 cut(s) 544, 658, 676, 1007
MhlI GDGCHC 4 cut(s) 132, 459, 784, 825
MluCI AATT 8 cut(s) 187, 202, 386, 448, 499, 842, 1037, 1131
MlyI GAGTC 7 cut(s) 72, 251, 662, 693, 745, 872, 1201
MmeI TCCRAC 2 cut(s) 379, 1169
MseI TTAA 3 cut(s) 492, 629, 966
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 5 cut(s) 208, 862, 977, 1029, 1226
MspR9I CCNGG 3 cut(s) 209, 863, 1018
Mva1269I GAATGC 1 cut(s) 434
MvaI CCWGG 1 cut(s) 1018
MvnI CGCG 2 cut(s) 852, 926
MwoI GCNNNNNNNGC 1 cut(s) 925
NciI CCSGG 2 cut(s) 209, 863
NdeII GATC 4 cut(s) 544, 658, 676, 1007
NlaIII CATG 6 cut(s) 322, 523, 690, 896, 1117, 1236
NlaIV GGNNCC 6 cut(s) 49, 654, 994, 1027, 1096, 1211
NmeAIII GCCGAG 2 cut(s) 246, 1150
NmuCI GTSAC 2 cut(s) 64, 566
NspI RCATGY 1 cut(s) 523
PagI TCATGA 1 cut(s) 1232
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PctI GAATGC 1 cut(s) 434
PfeI GAWTC 4 cut(s) 374, 973, 1218, 1229
PkrI GCNGC 6 cut(s) 317, 359, 434, 518, 839, 949
PleI GAGTC 7 cut(s) 71, 251, 662, 693, 745, 872, 1200
PpsI GAGTC 7 cut(s) 71, 251, 662, 693, 745, 872, 1200
Ppu21I YACGTR 1 cut(s) 56
PsiI TTATAA 1 cut(s) 42
Psp124BI GAGCTC 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 1016
PspFI CCCAGC 2 cut(s) 353, 1137
PspGI CCWGG 1 cut(s) 1016
PspN4I GGNNCC 6 cut(s) 49, 654, 994, 1027, 1096, 1211
PspPI GGNCC 4 cut(s) 286, 652, 914, 1031
PstI CTGCAG 1 cut(s) 437
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
SacI GAGCTC 1 cut(s) 459
SaqAI TTAA 3 cut(s) 492, 629, 966
SatI GCNGC 6 cut(s) 316, 358, 433, 517, 838, 948
Sau3AI GATC 4 cut(s) 544, 658, 676, 1007
Sau96I GGNCC 4 cut(s) 286, 652, 914, 1031
SchI GAGTC 7 cut(s) 72, 251, 662, 693, 745, 872, 1201
ScrFI CCNGG 3 cut(s) 209, 863, 1018
SduI GDGCHC 4 cut(s) 132, 459, 784, 825
SfaNI GCATC 1 cut(s) 457
SfcI CTRYAG 1 cut(s) 433
SinI GGWCC 2 cut(s) 652, 1031
SmlI CTYRAG 2 cut(s) 133, 806
SmoI CTYRAG 2 cut(s) 133, 806
SnaBI TACGTA 1 cut(s) 56
Sse9I AATT 8 cut(s) 187, 202, 386, 448, 499, 842, 1037, 1131
SsiI CCGC 2 cut(s) 540, 917
SspMI CTAG 2 cut(s) 884, 1052
SstI GAGCTC 1 cut(s) 459
StyD4I CCNGG 3 cut(s) 207, 861, 1016
StyI CCWWGG 1 cut(s) 648
TaaI ACNGT 3 cut(s) 79, 572, 1087
TaiI ACGT 2 cut(s) 58, 399
TaqI TCGA 1 cut(s) 547
TasI AATT 8 cut(s) 187, 202, 386, 448, 499, 842, 1037, 1131
TfiI GAWTC 4 cut(s) 374, 973, 1218, 1229
Tru1I TTAA 3 cut(s) 492, 629, 966
Tru9I TTAA 3 cut(s) 492, 629, 966
TscAI CASTG 1 cut(s) 1090
TseFI GTSAC 2 cut(s) 64, 566
TseI GCWGC 6 cut(s) 315, 357, 432, 516, 837, 947
Tsp45I GTSAC 2 cut(s) 64, 566
TspDTI ATGAA 4 cut(s) 85, 206, 746, 1033
TspGWI ACGGA 5 cut(s) 89, 758, 897, 1087, 1171
TspRI CASTG 1 cut(s) 1090
VpaK11BI GGWCC 2 cut(s) 652, 1031
XapI RAATTY 4 cut(s) 386, 448, 499, 1037
XceI RCATGY 1 cut(s) 523
XspI CTAG 2 cut(s) 884, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.