MD07G1140200.v1.1

tRNA-specific adenosine deaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005289
Physical Location & Seq
Reverse (-)
20344853 .. 20347040
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1140200.v1.1.491

Sequence Viewer

Length: 924 bp
ATGGCGTCGCCGCCCTGCTCCGTAACGCCGTCTTCTCCTTCTCCTTCTTCCTCAGAAACAGACTGGGCAACTACGGTCTCAGACAAAGTGTTGTCACTCTACAACTCTCTTCCCAAGAAAGGCAAGCCTCAGGGCCGCGAAGTCACCGTCTTGGCCGCTCTCTCGTCTCCTCCCCTTCTCAAGGGGGAATCTGCATTTACCCTTGTTCGTAACTATATTTGGTTAACTATATATGTACCTGATGTTTGTAATGTATGGTGTTTTTTCATATATGTGGGTTTGGTGGACCTAGGTTCCTCTATGTCTATGCACAGATTCAGTGTTCTTGCTCAAATGTATAGTGAGCAAAGTGATAACAACGGAAGCACGCAGTTTCAAAATGGTGATGCCAAAAACTTTATTTTTGAATTGGGCCCGGATGGTGATGGCGAAAGAAAATACTCAATGAATAAGAATTGGAATTTGCACTTGTACATATCACAACTACCATGTGGGGTTGCTTCTCCGAGTTCACTGTTGTCTCCTCCAAAAATTATTGGACCAAGAGAACGGGGTTCATCTTTGTACGAGCTTAACACTTCCATTGCAGAAGAGGCTCTGCCAAAGACTAATGGTAGTGATGCTTCACAACTGGTTGGCTCATTACAAAGAAAGCCTGGTCGTGGTGATACAACATTATCAGTTAGTTGCTCTGACAAGATAGCCCGCTGGAATGTTGTTGGAGTTCAAGGAGCCCTGCTGTCATTCTTTCTAAAACCTGTATACCTTTCTTCCATTACCATTGGACAATCCCCTAACAGCTCAGAAACCGTTCTCGTGGTTGATTACCTGAAAAAAGCATTGCATGATCGCATCCTACCGTTATCAAATGAGCTGATGAGTCCCTTTCAAGTGAAGCAGGTCTGTGAAATGTGTACTCTGTGA

Protein Analysis

308

Amino Acids

33.46

Weight (kDa)

6.82

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_deamin PF02137 130 - 302 4.7e-37 Adenosine-deaminase (editase) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 889
AccBSI CCGCTC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 762
AccII CGCG 1 cut(s) 138
AciI CCGC 4 cut(s) 11, 136, 156, 706
AcoI YGGCCR 1 cut(s) 153
AcsI RAATTY 1 cut(s) 460
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 237, 473, 566, 916
AfiI CCNNNNNNNGG 3 cut(s) 119, 181, 662
AgsI TTSAA 4 cut(s) 377, 407, 728, 890
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 3 cut(s) 571, 801, 874
AluI AGCT 3 cut(s) 571, 801, 874
Alw26I GTCTC 3 cut(s) 82, 171, 525
AoxI GGCC 3 cut(s) 133, 153, 412
ApaI GGGCCC 1 cut(s) 416
ApoI RAATTY 1 cut(s) 460
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
Asp700I GAANNNNTTC 1 cut(s) 810
AspA2I CCTAGG 1 cut(s) 289
AspS9I GGNCC 5 cut(s) 133, 286, 412, 413, 539
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 4 cut(s) 136, 395, 434, 677
AvaII GGWCC 2 cut(s) 286, 539
AvrII CCTAGG 1 cut(s) 289
AxyI CCTNAGG 1 cut(s) 129
BaeGI GKGCMC 1 cut(s) 416
BanII GRGCYC 2 cut(s) 416, 736
BarI GAAGNNNNNNTAC 2 cut(s) 607, 639
BauI CACGAG 1 cut(s) 815
BbsI GAAGAC 1 cut(s) 24
BccI CCATC 2 cut(s) 413, 419
BceAI ACGGC 1 cut(s) 13
BciT130I CCWGG 1 cut(s) 657
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 3 cut(s) 82, 171, 525
BfaI CTAG 1 cut(s) 290
BfuAI ACCTGC 1 cut(s) 889
BisI GCNGC 3 cut(s) 11, 136, 156
BlnI CCTAGG 1 cut(s) 289
BlsI GCNGC 3 cut(s) 12, 137, 157
Bme1390I CCNGG 2 cut(s) 416, 657
Bme18I GGWCC 2 cut(s) 286, 539
BmgT120I GGNCC 5 cut(s) 133, 286, 412, 413, 539
BmiI GGNNCC 3 cut(s) 295, 414, 733
BmrFI CCNGG 2 cut(s) 416, 657
BmrI ACTGGG 1 cut(s) 73
BmsI GCATC 3 cut(s) 376, 610, 861
BmuI ACTGGG 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 1 cut(s) 289
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 181, 662
Bse1I ACTGG 2 cut(s) 68, 636
Bse21I CCTNAGG 1 cut(s) 129
Bse3DI GCAATG 2 cut(s) 582, 839
BseBI CCWGG 1 cut(s) 657
BseDI CCNNGG 1 cut(s) 289
BseGI GGATG 2 cut(s) 424, 852
BseLI CCNNNNNNNGG 3 cut(s) 119, 181, 662
BseMI GCAATG 2 cut(s) 582, 839
BseMII CTCAG 4 cut(s) 66, 93, 143, 816
BseNI ACTGG 2 cut(s) 68, 636
BseRI GAGGAG 2 cut(s) 159, 513
BseSI GKGCMC 1 cut(s) 416
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 3 cut(s) 135, 155, 414
BsiSI CCGG 1 cut(s) 416
BslFI GGGAC 1 cut(s) 867
BslI CCNNNNNNNGG 3 cut(s) 119, 181, 662
BsmAI GTCTC 3 cut(s) 82, 171, 525
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 867
BsnI GGCC 3 cut(s) 135, 155, 414
Bso31I GGTCTC 1 cut(s) 82
Bsp120I GGGCCC 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 416, 736
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 1 cut(s) 847
BspACI CCGC 4 cut(s) 11, 136, 156, 706
BspANI GGCC 3 cut(s) 135, 155, 414
BspCNI CTCAG 4 cut(s) 65, 92, 142, 815
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 3 cut(s) 295, 414, 733
BspMI ACCTGC 1 cut(s) 889
BspTNI GGTCTC 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 582, 839
BsrGI TGTACA 1 cut(s) 471
BsrI ACTGG 2 cut(s) 68, 636
BssECI CCNNGG 1 cut(s) 289
BssMI GATC 1 cut(s) 847
BssNAI GTATAC 1 cut(s) 763
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 815
BssT1I CCWWGG 1 cut(s) 289
Bst1107I GTATAC 1 cut(s) 763
Bst2BI CACGAG 1 cut(s) 815
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 5 cut(s) 76, 148, 516, 811, 861
Bst6I CTCTTC 2 cut(s) 114, 585
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 471
BstC8I GCNNGC 3 cut(s) 125, 368, 706
BstDEI CTNAG 4 cut(s) 52, 79, 129, 802
BstENI CCTNNNNNAGG 1 cut(s) 179
BstF5I GGATG 2 cut(s) 424, 852
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 1 cut(s) 850
BstMAI GTCTC 3 cut(s) 82, 171, 525
BstMBI GATC 1 cut(s) 847
BstMWI GCNNNNNNNGC 1 cut(s) 593
BstNI CCWGG 1 cut(s) 657
BstSCI CCNGG 2 cut(s) 414, 655
BstSLI GKGCMC 1 cut(s) 416
BstUI CGCG 1 cut(s) 138
BstV2I GAAGAC 1 cut(s) 24
BstZ17I GTATAC 1 cut(s) 763
Bsu36I CCTNAGG 1 cut(s) 129
BsuRI GGCC 3 cut(s) 135, 155, 414
BtsCI GGATG 2 cut(s) 424, 852
BtsIMutI CAGTG 2 cut(s) 325, 512
BveI ACCTGC 1 cut(s) 889
Cac8I GCNNGC 3 cut(s) 125, 368, 706
Cfr13I GGNCC 5 cut(s) 133, 286, 412, 413, 539
Csp6I GTAC 4 cut(s) 236, 472, 565, 915
CviAII CATG 2 cut(s) 489, 845
CviQI GTAC 4 cut(s) 236, 472, 565, 915
DdeI CTNAG 4 cut(s) 52, 79, 129, 802
DpnI GATC 1 cut(s) 849
DpnII GATC 1 cut(s) 847
EaeI YGGCCR 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 114, 585
EarI CTCTTC 2 cut(s) 114, 585
Eco130I CCWWGG 1 cut(s) 289
Eco24I GRGCYC 2 cut(s) 416, 736
Eco31I GGTCTC 1 cut(s) 82
Eco47I GGWCC 2 cut(s) 286, 539
Eco81I CCTNAGG 1 cut(s) 129
EcoNI CCTNNNNNAGG 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 655
EcoT14I CCWWGG 1 cut(s) 289
EcoT38I GRGCYC 2 cut(s) 416, 736
ErhI CCWWGG 1 cut(s) 289
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 2 cut(s) 492, 848
FaqI GGGAC 1 cut(s) 867
FatI CATG 2 cut(s) 488, 844
FauI CCCGC 1 cut(s) 713
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 3 cut(s) 11, 136, 156
FokI GGATG 2 cut(s) 431, 839
FriOI GRGCYC 2 cut(s) 416, 736
Fsp4HI GCNGC 3 cut(s) 11, 136, 156
FspBI CTAG 1 cut(s) 290
GluI GCNGC 3 cut(s) 11, 136, 156
HaeIII GGCC 3 cut(s) 135, 155, 414
HapII CCGG 1 cut(s) 416
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 492, 848
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 3 cut(s) 188, 315, 880
HpaI GTTAAC 1 cut(s) 225
HpaII CCGG 1 cut(s) 416
HphI GGTGA 4 cut(s) 136, 395, 434, 677
Hpy166II GTNNAC 5 cut(s) 225, 286, 512, 763, 915
Hpy188I TCNGA 5 cut(s) 55, 82, 507, 694, 805
Hpy8I GTNNAC 5 cut(s) 225, 286, 512, 763, 915
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 48, 54, 185
HpyCH4III ACNGT 5 cut(s) 76, 148, 516, 811, 861
HpyCH4V TGCA 5 cut(s) 194, 310, 466, 587, 844
HpyF10VI GCNNNNNNNGC 1 cut(s) 593
HpyF3I CTNAG 4 cut(s) 52, 79, 129, 802
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 492, 848
KspAI GTTAAC 1 cut(s) 225
Kzo9I GATC 1 cut(s) 847
LmnI GCTCC 2 cut(s) 23, 731
LweI GCATC 3 cut(s) 376, 610, 861
MaeI CTAG 1 cut(s) 290
MaeIII GTNAC 4 cut(s) 22, 93, 142, 209
MalI GATC 1 cut(s) 849
MbiI CCGCTC 1 cut(s) 158
MboI GATC 1 cut(s) 847
MboII GAAGA 5 cut(s) 24, 39, 101, 602, 762
MhlI GDGCHC 2 cut(s) 416, 736
MluCI AATT 4 cut(s) 407, 454, 460, 531
MlyI GAGTC 1 cut(s) 889
MmeI TCCRAC 1 cut(s) 700
MnlI CCTC 6 cut(s) 61, 138, 180, 307, 534, 586
MroXI GAANNNNTTC 1 cut(s) 810
MseI TTAA 2 cut(s) 224, 573
MslI CAYNNNNRTG 1 cut(s) 272
MspA1I CMGCKG 1 cut(s) 708
MspI CCGG 1 cut(s) 416
MspR9I CCNGG 2 cut(s) 416, 657
MvaI CCWGG 1 cut(s) 657
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 1 cut(s) 593
NciI CCSGG 1 cut(s) 416
NdeII GATC 1 cut(s) 847
NlaIII CATG 2 cut(s) 492, 848
NlaIV GGNNCC 3 cut(s) 295, 414, 733
NmuCI GTSAC 2 cut(s) 93, 142
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 2 cut(s) 188, 315
PkrI GCNGC 3 cut(s) 12, 137, 157
PleI GAGTC 1 cut(s) 888
PpsI GAGTC 1 cut(s) 888
Psp6I CCWGG 1 cut(s) 655
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 3 cut(s) 295, 414, 733
PspOMI GGGCCC 1 cut(s) 412
PspPI GGNCC 5 cut(s) 133, 286, 412, 413, 539
RsaI GTAC 4 cut(s) 237, 473, 566, 916
RsaNI GTAC 4 cut(s) 236, 472, 565, 915
RseI CAYNNNNRTG 1 cut(s) 272
SaqAI TTAA 2 cut(s) 224, 573
SatI GCNGC 3 cut(s) 11, 136, 156
Sau3AI GATC 1 cut(s) 847
Sau96I GGNCC 5 cut(s) 133, 286, 412, 413, 539
SchI GAGTC 1 cut(s) 889
ScrFI CCNGG 2 cut(s) 416, 657
SduI GDGCHC 2 cut(s) 416, 736
SfaNI GCATC 3 cut(s) 376, 610, 861
SinI GGWCC 2 cut(s) 286, 539
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 4 cut(s) 407, 454, 460, 531
SsiI CCGC 4 cut(s) 11, 136, 156, 706
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 2 cut(s) 414, 655
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 5 cut(s) 76, 148, 516, 811, 861
TasI AATT 4 cut(s) 407, 454, 460, 531
TatI WGTACW 2 cut(s) 471, 914
TauI GCSGC 3 cut(s) 13, 138, 158
TfiI GAWTC 2 cut(s) 188, 315
Tru1I TTAA 2 cut(s) 224, 573
Tru9I TTAA 2 cut(s) 224, 573
TscAI CASTG 2 cut(s) 325, 519
TseFI GTSAC 2 cut(s) 93, 142
Tsp45I GTSAC 2 cut(s) 93, 142
TspDTI ATGAA 3 cut(s) 256, 461, 546
TspGWI ACGGA 2 cut(s) 10, 375
TspRI CASTG 2 cut(s) 325, 519
VpaK11BI GGWCC 2 cut(s) 286, 539
XagI CCTNNNNNAGG 1 cut(s) 179
XapI RAATTY 1 cut(s) 460
XmaJI CCTAGG 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 762
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.