Rh1BG223800

tRNA-specific adenosine deaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0005289
Physical Location & Seq
Forward (+)
34280738 .. 34282307
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG223800.1

Sequence Viewer

Length: 921 bp
ATGACGTCGCCTCCCTGCTTCGAAGCGGCGTCGTTTCGTCCTCCTTCTCCAGATTCAGATTGGGGACACAAAGTCTCAGACAAGGTGCTGTCACTCTACAACTCCCTCCCTAAGAAAGGCAAGCCTCAGGGCCGCGAAGTCACCGTCTTGGCCGCCTTCCTCCTCTCCTCTCCTCTTCAAGAATTGGAAGTTGTAGCATTGGGAACCGGAACCAAATGCGTCGGCCATTCACTCCTCAGCCCCCGCGGCGATATCGTCAACGATTCCCACGCCGAGGTCATCGCTCGCCGCGCTTTAATCAGGTTCTTCTATGCACAGATACAATGTGTTTCTCAAATTTATAGTAGGCAAAGAGATAACAATGGAAGCAAAGAGTTGCGAAACGATGATGTGAAAAAGTTTATTTTTGAATTGGACCCTGATGATGGGCAAGGAAAATACACAATGAGGAAGGGTTGGAAATTGCATATGTATATATCACAACTACCATGTGGGATTGCTTCGCCGAGTTCATTGTTGTCTCCCCCGAAAAATATCTCACCTAGCGGAGGGGTTTCATTACTGGAGGAGCTTAATGAGTCCATAAATAAGGAGGCTTTGATTGAGAATAATGGTGACGTTTCACAACTAGTTGGGACGGTTCAAAGAAAGCCTGGTCGTGGTGATACAACATTATCAGTTAGCTGCTCTGACAAGATAGCCCGTTGGAATGTCGTTGGAGTTCAAGGAGCTTTGCTTTCCTTCTTTCTTCAACCTGTATACCTTTCTTCCATTACTGTTGGACAATCCCCTATCAACTCCGAAAAGGTTCTTGTGGTTGATTGTCTGAAACAAGCTTTGCATGATCGTGTCCTACCATTATCAAGCGAGCTGACCAGTCCCTTTCAGGTGAACCAGGTCTGTGAAGTGTCTACTCTGTGA

Protein Analysis

306

Amino Acids

33.42

Weight (kDa)

7.58

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_deamin PF02137 66 - 301 4.1e-59 Adenosine-deaminase (editase) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccI GTMKAC 2 cut(s) 759, 911
AccII CGCG 3 cut(s) 135, 246, 291
AciI CCGC 7 cut(s) 26, 133, 153, 244, 246, 289, 546
AcoI YGGCCR 2 cut(s) 150, 223
AcsI RAATTY 1 cut(s) 336
AcyI GRCGYC 2 cut(s) 5, 29
AfiI CCNNNNNNNGG 5 cut(s) 116, 274, 425, 548, 659
AgsI TTSAA 5 cut(s) 179, 410, 644, 725, 752
AhdI GACNNNNNGTC 1 cut(s) 71
AhlI ACTAGT 1 cut(s) 628
AjnI CCWGG 2 cut(s) 652, 894
AluBI AGCT 5 cut(s) 571, 684, 731, 836, 871
AluI AGCT 5 cut(s) 571, 684, 731, 836, 871
Alw26I GTCTC 2 cut(s) 79, 525
AoxI GGCC 3 cut(s) 130, 150, 223
ApeKI GCWGC 1 cut(s) 684
ApoI RAATTY 1 cut(s) 336
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
Asp700I GAANNNNTTC 1 cut(s) 807
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 130, 415
AsuHPI GGTGA 5 cut(s) 133, 531, 626, 674, 901
AsuII TTCGAA 1 cut(s) 21
AvaII GGWCC 1 cut(s) 415
AxyI CCTNAGG 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 1 cut(s) 671
BccI CCATC 1 cut(s) 419
BciT130I CCWGG 2 cut(s) 654, 896
BcoDI GTCTC 2 cut(s) 79, 525
BcuI ACTAGT 1 cut(s) 628
BfaI CTAG 2 cut(s) 543, 629
BglI GCCNNNNNGGC 1 cut(s) 246
BisI GCNGC 6 cut(s) 27, 133, 153, 247, 289, 685
BlsI GCNGC 6 cut(s) 28, 134, 154, 248, 290, 686
Bme1390I CCNGG 2 cut(s) 654, 896
Bme18I GGWCC 1 cut(s) 415
BmeRI GACNNNNNGTC 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 130, 415
BmiI GGNNCC 3 cut(s) 205, 211, 417
BmrFI CCNGG 2 cut(s) 654, 896
BpmI CTGGAG 2 cut(s) 33, 584
Bpu10I CCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 21
BsaHI GRCGYC 2 cut(s) 5, 29
BsaJI CCNNGG 2 cut(s) 244, 273
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 5 cut(s) 116, 274, 425, 548, 659
Bse1I ACTGG 2 cut(s) 567, 876
Bse21I CCTNAGG 1 cut(s) 126
BseBI CCWGG 2 cut(s) 654, 896
BseDI CCNNGG 2 cut(s) 244, 273
BseLI CCNNNNNNNGG 5 cut(s) 116, 274, 425, 548, 659
BseMII CTCAG 3 cut(s) 90, 140, 250
BseNI ACTGG 2 cut(s) 567, 876
BseRI GAGGAG 5 cut(s) 152, 157, 162, 224, 581
BseXI GCAGC 1 cut(s) 671
Bsh1236I CGCG 3 cut(s) 135, 246, 291
BshFI GGCC 3 cut(s) 132, 152, 225
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 3 cut(s) 78, 649, 864
BslI CCNNNNNNNGG 5 cut(s) 116, 274, 425, 548, 659
BsmAI GTCTC 2 cut(s) 79, 525
BsmFI GGGAC 3 cut(s) 78, 649, 864
BsnI GGCC 3 cut(s) 132, 152, 225
Bsp119I TTCGAA 1 cut(s) 21
Bsp143I GATC 1 cut(s) 844
BspACI CCGC 7 cut(s) 26, 133, 153, 244, 246, 289, 546
BspANI GGCC 3 cut(s) 132, 152, 225
BspCNI CTCAG 3 cut(s) 89, 139, 249
BspFNI CGCG 3 cut(s) 135, 246, 291
BspLI GGNNCC 3 cut(s) 205, 211, 417
BspT104I TTCGAA 1 cut(s) 21
BsrI ACTGG 2 cut(s) 567, 876
BssECI CCNNGG 2 cut(s) 244, 273
BssMI GATC 1 cut(s) 844
BssNAI GTATAC 1 cut(s) 760
BssNI GRCGYC 2 cut(s) 5, 29
Bst1107I GTATAC 1 cut(s) 760
Bst2UI CCWGG 2 cut(s) 654, 896
Bst4CI ACNGT 3 cut(s) 145, 640, 778
Bst6I CTCTTC 1 cut(s) 180
BstACI GRCGYC 2 cut(s) 5, 29
BstBI TTCGAA 1 cut(s) 21
BstC8I GCNNGC 3 cut(s) 122, 286, 869
BstDEI CTNAG 4 cut(s) 76, 111, 126, 236
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 2 cut(s) 114, 546
BstFNI CGCG 3 cut(s) 135, 246, 291
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 1 cut(s) 847
BstMAI GTCTC 2 cut(s) 79, 525
BstMBI GATC 1 cut(s) 844
BstMWI GCNNNNNNNGC 2 cut(s) 246, 290
BstNI CCWGG 2 cut(s) 654, 896
BstSCI CCNGG 2 cut(s) 652, 894
BstUI CGCG 3 cut(s) 135, 246, 291
BstV1I GCAGC 1 cut(s) 671
BstZ17I GTATAC 1 cut(s) 760
Bsu36I CCTNAGG 1 cut(s) 126
BsuRI GGCC 3 cut(s) 132, 152, 225
BtgI CCRYGG 1 cut(s) 244
BtgZI GCGATG 1 cut(s) 265
Cac8I GCNNGC 3 cut(s) 122, 286, 869
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 2 cut(s) 130, 415
Cfr42I CCGCGG 1 cut(s) 247
CseI GACGC 2 cut(s) 18, 208
CsiI ACCWGGT 1 cut(s) 894
CviAII CATG 2 cut(s) 489, 842
DdeI CTNAG 4 cut(s) 76, 111, 126, 236
DpnI GATC 1 cut(s) 846
DpnII GATC 1 cut(s) 844
DriI GACNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 2 cut(s) 150, 223
Eam1104I CTCTTC 1 cut(s) 180
Eam1105I GACNNNNNGTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 415
Eco81I CCTNAGG 1 cut(s) 126
EcoNI CCTNNNNNAGG 2 cut(s) 114, 546
EcoRII CCWGG 2 cut(s) 652, 894
EcoRV GATATC 1 cut(s) 253
FaeI CATG 2 cut(s) 492, 845
FaqI GGGAC 3 cut(s) 78, 649, 864
FatI CATG 2 cut(s) 488, 841
FauI CCCGC 1 cut(s) 251
FauNDI CATATG 1 cut(s) 468
FblI GTMKAC 2 cut(s) 759, 911
Fnu4HI GCNGC 6 cut(s) 27, 133, 153, 247, 289, 685
Fsp4HI GCNGC 6 cut(s) 27, 133, 153, 247, 289, 685
FspBI CTAG 2 cut(s) 543, 629
GlaI GCGC 1 cut(s) 292
GluI GCNGC 6 cut(s) 27, 133, 153, 247, 289, 685
GsuI CTGGAG 2 cut(s) 33, 584
HaeIII GGCC 3 cut(s) 132, 152, 225
HapII CCGG 1 cut(s) 207
HgaI GACGC 2 cut(s) 18, 208
HhaI GCGC 1 cut(s) 293
Hin1I GRCGYC 2 cut(s) 5, 29
Hin1II CATG 2 cut(s) 492, 845
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 3 cut(s) 53, 263, 578
HpaII CCGG 1 cut(s) 207
HphI GGTGA 5 cut(s) 133, 531, 626, 674, 901
Hpy166II GTNNAC 4 cut(s) 259, 760, 892, 912
Hpy188I TCNGA 5 cut(s) 58, 79, 691, 802, 828
Hpy188III TCNNGA 2 cut(s) 50, 179
Hpy8I GTNNAC 4 cut(s) 259, 760, 892, 912
Hpy99I CGWCG 3 cut(s) 10, 34, 224
HpyAV CCTTC 4 cut(s) 54, 166, 445, 751
HpyCH4III ACNGT 3 cut(s) 145, 640, 778
HpyCH4IV ACGT 2 cut(s) 5, 618
HpyCH4V TGCA 3 cut(s) 314, 466, 841
HpyF10VI GCNNNNNNNGC 2 cut(s) 246, 290
HpyF3I CTNAG 4 cut(s) 76, 111, 126, 236
HpySE526I ACGT 2 cut(s) 5, 618
Hsp92I GRCGYC 2 cut(s) 5, 29
Hsp92II CATG 2 cut(s) 492, 845
HspAI GCGC 1 cut(s) 291
KspI CCGCGG 1 cut(s) 247
Kzo9I GATC 1 cut(s) 844
LmnI GCTCC 2 cut(s) 568, 728
Lsp1109I GCAGC 1 cut(s) 671
MabI ACCWGGT 1 cut(s) 894
MaeI CTAG 2 cut(s) 543, 629
MaeII ACGT 2 cut(s) 5, 618
MaeIII GTNAC 3 cut(s) 90, 139, 614
MalI GATC 1 cut(s) 846
MboI GATC 1 cut(s) 844
MboII GAAGA 4 cut(s) 167, 298, 740, 759
MluCI AATT 4 cut(s) 182, 336, 410, 461
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 4 cut(s) 437, 686, 697, 760
MroXI GAANNNNTTC 1 cut(s) 807
MseI TTAA 2 cut(s) 296, 573
MslI CAYNNNNRTG 1 cut(s) 846
MspA1I CMGCKG 1 cut(s) 246
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 654, 896
MvaI CCWGG 2 cut(s) 654, 896
MvnI CGCG 3 cut(s) 135, 246, 291
MwoI GCNNNNNNNGC 2 cut(s) 246, 290
NdeI CATATG 1 cut(s) 468
NdeII GATC 1 cut(s) 844
NlaIII CATG 2 cut(s) 492, 845
NlaIV GGNNCC 3 cut(s) 205, 211, 417
NmeAIII GCCGAG 2 cut(s) 298, 531
NmuCI GTSAC 3 cut(s) 90, 139, 614
NspV TTCGAA 1 cut(s) 21
PdmI GAANNNNTTC 1 cut(s) 807
PfeI GAWTC 2 cut(s) 53, 263
PkrI GCNGC 6 cut(s) 28, 134, 154, 248, 290, 686
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
Psp6I CCWGG 2 cut(s) 652, 894
PspGI CCWGG 2 cut(s) 652, 894
PspN4I GGNNCC 3 cut(s) 205, 211, 417
PspPI GGNCC 2 cut(s) 130, 415
RseI CAYNNNNRTG 1 cut(s) 846
SacII CCGCGG 1 cut(s) 247
SaqAI TTAA 2 cut(s) 296, 573
SatI GCNGC 6 cut(s) 27, 133, 153, 247, 289, 685
Sau3AI GATC 1 cut(s) 844
Sau96I GGNCC 2 cut(s) 130, 415
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 2 cut(s) 654, 896
SexAI ACCWGGT 1 cut(s) 894
Sfr303I CCGCGG 1 cut(s) 247
SfuI TTCGAA 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 247
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 1 cut(s) 846
SpeI ACTAGT 1 cut(s) 628
Sse9I AATT 4 cut(s) 182, 336, 410, 461
SsiI CCGC 7 cut(s) 26, 133, 153, 244, 246, 289, 546
SspMI CTAG 2 cut(s) 543, 629
StyD4I CCNGG 2 cut(s) 652, 894
TaaI ACNGT 3 cut(s) 145, 640, 778
TaiI ACGT 2 cut(s) 8, 621
TaqI TCGA 1 cut(s) 21
TasI AATT 4 cut(s) 182, 336, 410, 461
TauI GCSGC 5 cut(s) 29, 135, 155, 249, 291
TfiI GAWTC 2 cut(s) 53, 263
Tru1I TTAA 2 cut(s) 296, 573
Tru9I TTAA 2 cut(s) 296, 573
TseFI GTSAC 3 cut(s) 90, 139, 614
TseI GCWGC 1 cut(s) 684
Tsp45I GTSAC 3 cut(s) 90, 139, 614
TspDTI ATGAA 2 cut(s) 501, 546
VpaK11BI GGWCC 1 cut(s) 415
XagI CCTNNNNNAGG 2 cut(s) 114, 546
XapI RAATTY 1 cut(s) 336
XcmI CCANNNNNNNNNTGG 1 cut(s) 57
XmiI GTMKAC 2 cut(s) 759, 911
XmnI GAANNNNTTC 1 cut(s) 807
XspI CTAG 2 cut(s) 543, 629
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.