Rroxscaffold_4G00301240

tRNA-specific adenosine deaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005289
Physical Location & Seq
Reverse (-)
21649521 .. 21653237
3717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301240.1

Sequence Viewer

Length: 1293 bp
ATGACGTCGCCTCCCTGCTTAGAAACGGCGTCGTTTTGTCCTTCTTCTACAGACTCAGATTGGGGACACACAGTCTCAGACAAGGTGCTCTCACTCTACAACTCCCTCCCTAAGAAAGGCAAGCCTCAGGGCCGCGAAGTCACCGTCTTGGCCGCCTTCCTCCTCTCCTCTCCTCTTCAAGAATTGGAAGTTGTAGCATTGGGAACCGGAACCAAATGCGTCGGCCGTTCACTCCTCAGCCCCCACGGCGATATCGTCAACGATTCCCACGCCGAGGTCATCGCTCGCCGCGCTTTAATCAGGTTCTTCTATGCACAGATACAATGTCTTTCTGAAATTTATACTAGGCAAAGAGATAACAATGGAGGCAAACAGTTGCGAAACGATGATGCCAAAAAGTTCATTTTTGAATTGGACCCTGATGATGGGCAAGGAAAATATAGAATGAGGAAGGGTTGGAAATTGCACATGTATATATCGCAGCTACCTTGTGGGATTGCTTCGCCGAGTTCATTGTTGTCTCCCCCGAAAAATATCTCACCTAGCGGAGGAGGTTCATTACTGGAGGAGCTTAATGAGTCCATAAATAAGGAGGCTTTGATTGAGAATAATGGTGACTTTTCACAACTAATTGGGACGGTTCAAAGAAAGCCTGGTCGTGGTGATACAACATTATCAGTTAGCTGCTCTGACAAGATTGCCCGTTGGAATGTCGTTGGAGTTCAAGGAGCTTTGCTTTCCTTCTTTCTTCAACCTGTATACCTTTCTTCCATTACTGTTGGACAATCCCCTATCAACTCTGAAAAGGTTCTTGTGGTTGATTGTCTGAAACAAGCTTTGCATGATCGTGTCCTACCATTATCAAGCGAGGTGACCAGTCCCTTTCAGGTGAACCAGCCACTACTCTGGGAAGCTCCAATGCCACCAAAGGAATTTCAACATTCTGAGACCGCTTTGACCACTATAACATGCGGGTATTCAATATGTTGGAATAAGTCTGGTTTGCATGAAGTCATTCTTGGAACCATAGGAAGAAAGCAAGGCGCTGCAAAAGGAGCACGCAGTCCGTCTACTGAATCCCCTATATGCAAAAAACGGCTCTTGGAGATTTTCTTGTCCCTAAGGAATGAATGCCCCTCCAATATCCATGTCAATGAGATTTCGTATCGAGAAATTAAGGAAAGGGCTCATGAATACAATGCAATGTCAAAAGTTCTAAAAAGAAGATGTACCTTTAGTAATTGGCTATTGAAGCCCCCAAATTTGGAGGCATTCTCTGCTATGGGAGAGTGA

Protein Analysis

430

Amino Acids

47.44

Weight (kDa)

8.63

Isoelectric Point (pI)

56.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_deamin PF02137 66 - 419 2.1e-80 Adenosine-deaminase (editase) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccI GTMKAC 2 cut(s) 759, 1070
AccII CGCG 2 cut(s) 135, 291
AciI CCGC 6 cut(s) 133, 153, 289, 546, 951, 972
AcoI YGGCCR 2 cut(s) 150, 223
AcsI RAATTY 3 cut(s) 336, 932, 1261
AcyI GRCGYC 2 cut(s) 5, 29
AfaI GTAC 1 cut(s) 1231
AfiI CCNNNNNNNGG 6 cut(s) 116, 274, 425, 548, 659, 1264
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 8 cut(s) 179, 410, 644, 725, 752, 938, 981, 1252
AhdI GACNNNNNGTC 1 cut(s) 71
AjnI CCWGG 1 cut(s) 652
AjuI GAANNNNNNNTTGG 2 cut(s) 1002, 1034
AluBI AGCT 6 cut(s) 484, 571, 684, 731, 836, 914
AluI AGCT 6 cut(s) 484, 571, 684, 731, 836, 914
Alw21I GWGCWC 2 cut(s) 90, 1060
Alw26I GTCTC 3 cut(s) 79, 525, 941
AoxI GGCC 3 cut(s) 130, 150, 223
ApeKI GCWGC 3 cut(s) 481, 684, 1046
ApoI RAATTY 3 cut(s) 336, 932, 1261
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
Asp700I GAANNNNTTC 2 cut(s) 807, 1014
AspLEI GCGC 2 cut(s) 293, 1046
AspS9I GGNCC 2 cut(s) 130, 415
AsuHPI GGTGA 6 cut(s) 133, 531, 626, 674, 883, 901
AvaII GGWCC 1 cut(s) 415
AxyI CCTNAGG 2 cut(s) 126, 1121
BanII GRGCYC 1 cut(s) 1189
Bbv12I GWGCWC 2 cut(s) 90, 1060
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 3 cut(s) 493, 671, 1033
BccI CCATC 1 cut(s) 419
BceAI ACGGC 4 cut(s) 42, 210, 262, 1112
BciT130I CCWGG 1 cut(s) 654
BcoDI GTCTC 3 cut(s) 79, 525, 941
BfaI CTAG 2 cut(s) 345, 543
BfmI CTRYAG 1 cut(s) 48
BfoI RGCGCY 1 cut(s) 1047
BglI GCCNNNNNGGC 1 cut(s) 246
BisI GCNGC 6 cut(s) 133, 153, 289, 482, 685, 1047
BlsI GCNGC 6 cut(s) 134, 154, 290, 483, 686, 1048
Bme1390I CCNGG 1 cut(s) 654
Bme18I GGWCC 1 cut(s) 415
BmeRI GACNNNNNGTC 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 130, 415
BmiI GGNNCC 4 cut(s) 205, 211, 417, 1024
BmrFI CCNGG 1 cut(s) 654
BmsI GCATC 1 cut(s) 379
BplI GAGNNNNNCTC 2 cut(s) 1259, 1291
BpmI CTGGAG 1 cut(s) 584
Bpu10I CCTNAGC 1 cut(s) 236
BsaHI GRCGYC 2 cut(s) 5, 29
BsaI GGTCTC 1 cut(s) 941
BsaJI CCNNGG 2 cut(s) 244, 273
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 6 cut(s) 116, 274, 425, 548, 659, 1264
Bse1I ACTGG 2 cut(s) 567, 876
Bse21I CCTNAGG 2 cut(s) 126, 1121
Bse3DI GCAATG 1 cut(s) 1209
BseBI CCWGG 1 cut(s) 654
BseDI CCNNGG 2 cut(s) 244, 273
BseLI CCNNNNNNNGG 6 cut(s) 116, 274, 425, 548, 659, 1264
BseMI GCAATG 1 cut(s) 1209
BseMII CTCAG 5 cut(s) 69, 90, 140, 250, 936
BseNI ACTGG 2 cut(s) 567, 876
BseRI GAGGAG 6 cut(s) 152, 157, 162, 224, 564, 581
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 3 cut(s) 493, 671, 1033
Bsh1236I CGCG 2 cut(s) 135, 291
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 3 cut(s) 132, 152, 225
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 90, 1060
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 4 cut(s) 78, 649, 864, 1102
BslI CCNNNNNNNGG 6 cut(s) 116, 274, 425, 548, 659, 1264
BsmAI GTCTC 3 cut(s) 79, 525, 941
BsmFI GGGAC 4 cut(s) 78, 649, 864, 1102
BsmI GAATGC 2 cut(s) 1136, 1271
BsnI GGCC 3 cut(s) 132, 152, 225
Bso31I GGTCTC 1 cut(s) 941
Bsp1286I GDGCHC 3 cut(s) 90, 1060, 1189
Bsp143I GATC 1 cut(s) 844
BspACI CCGC 6 cut(s) 133, 153, 289, 546, 951, 972
BspANI GGCC 3 cut(s) 132, 152, 225
BspCNI CTCAG 5 cut(s) 68, 89, 139, 249, 937
BspFNI CGCG 2 cut(s) 135, 291
BspHI TCATGA 1 cut(s) 1189
BspLI GGNNCC 4 cut(s) 205, 211, 417, 1024
BspTNI GGTCTC 1 cut(s) 941
BsrDI GCAATG 1 cut(s) 1209
BsrI ACTGG 2 cut(s) 567, 876
BssECI CCNNGG 2 cut(s) 244, 273
BssMI GATC 1 cut(s) 844
BssNAI GTATAC 1 cut(s) 760
BssNI GRCGYC 2 cut(s) 5, 29
Bst1107I GTATAC 1 cut(s) 760
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 5 cut(s) 73, 145, 375, 640, 778
Bst6I CTCTTC 1 cut(s) 180
BstACI GRCGYC 2 cut(s) 5, 29
BstAPI GCANNNNNTGC 1 cut(s) 1277
BstC8I GCNNGC 3 cut(s) 122, 286, 1060
BstDEI CTNAG 8 cut(s) 19, 55, 76, 111, 126, 236, 945, 1121
BstDSI CCRYGG 1 cut(s) 244
BstEII GGTNACC 1 cut(s) 871
BstENI CCTNNNNNAGG 2 cut(s) 114, 546
BstFNI CGCG 2 cut(s) 135, 291
BstH2I RGCGCY 1 cut(s) 1047
BstHHI GCGC 2 cut(s) 293, 1046
BstKTI GATC 1 cut(s) 847
BstMAI GTCTC 3 cut(s) 79, 525, 941
BstMBI GATC 1 cut(s) 844
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 5 cut(s) 246, 290, 1055, 1252, 1277
BstNI CCWGG 1 cut(s) 654
BstNSI RCATGY 2 cut(s) 472, 972
BstPI GGTNACC 1 cut(s) 871
BstSCI CCNGG 1 cut(s) 652
BstSFI CTRYAG 1 cut(s) 48
BstUI CGCG 2 cut(s) 135, 291
BstV1I GCAGC 3 cut(s) 493, 671, 1033
BstXI CCANNNNNNTGG 1 cut(s) 906
BstZ17I GTATAC 1 cut(s) 760
BstZI CGGCCG 1 cut(s) 223
Bsu36I CCTNAGG 2 cut(s) 126, 1121
BsuRI GGCC 3 cut(s) 132, 152, 225
BtgI CCRYGG 1 cut(s) 244
BtgZI GCGATG 1 cut(s) 265
Cac8I GCNNGC 3 cut(s) 122, 286, 1060
CciI TCATGA 1 cut(s) 1189
CfoI GCGC 2 cut(s) 293, 1046
Cfr13I GGNCC 2 cut(s) 130, 415
CseI GACGC 2 cut(s) 18, 208
Csp6I GTAC 1 cut(s) 1230
CviAII CATG 6 cut(s) 469, 842, 969, 1007, 1148, 1190
CviQI GTAC 1 cut(s) 1230
DdeI CTNAG 8 cut(s) 19, 55, 76, 111, 126, 236, 945, 1121
DpnI GATC 1 cut(s) 846
DpnII GATC 1 cut(s) 844
DriI GACNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 2 cut(s) 150, 223
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 180
Eam1105I GACNNNNNGTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 180
EclXI CGGCCG 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 1189
Eco31I GGTCTC 1 cut(s) 941
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 415
Eco52I CGGCCG 1 cut(s) 223
Eco81I CCTNAGG 2 cut(s) 126, 1121
Eco91I GGTNACC 1 cut(s) 871
EcoNI CCTNNNNNAGG 2 cut(s) 114, 546
EcoO65I GGTNACC 1 cut(s) 871
EcoRII CCWGG 1 cut(s) 652
EcoRV GATATC 1 cut(s) 253
EcoT38I GRGCYC 1 cut(s) 1189
FaeI CATG 6 cut(s) 472, 845, 972, 1010, 1151, 1193
FalI AAGNNNNNCTT 2 cut(s) 1002, 1034
FaqI GGGAC 4 cut(s) 78, 649, 864, 1102
FatI CATG 6 cut(s) 468, 841, 968, 1006, 1147, 1189
FauI CCCGC 1 cut(s) 965
FblI GTMKAC 2 cut(s) 759, 1070
Fnu4HI GCNGC 6 cut(s) 133, 153, 289, 482, 685, 1047
FriOI GRGCYC 1 cut(s) 1189
Fsp4HI GCNGC 6 cut(s) 133, 153, 289, 482, 685, 1047
FspBI CTAG 2 cut(s) 345, 543
GlaI GCGC 2 cut(s) 292, 1045
GluI GCNGC 6 cut(s) 133, 153, 289, 482, 685, 1047
GsuI CTGGAG 1 cut(s) 584
HaeII RGCGCY 1 cut(s) 1047
HaeIII GGCC 3 cut(s) 132, 152, 225
HapII CCGG 1 cut(s) 207
HgaI GACGC 2 cut(s) 18, 208
HhaI GCGC 2 cut(s) 293, 1046
Hin1I GRCGYC 2 cut(s) 5, 29
Hin1II CATG 6 cut(s) 472, 845, 972, 1010, 1151, 1193
Hin6I GCGC 2 cut(s) 291, 1044
HinP1I GCGC 2 cut(s) 291, 1044
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 4 cut(s) 53, 263, 578, 1076
HpaII CCGG 1 cut(s) 207
HphI GGTGA 6 cut(s) 133, 531, 626, 674, 883, 901
Hpy166II GTNNAC 5 cut(s) 230, 259, 760, 892, 1071
Hpy188I TCNGA 7 cut(s) 58, 79, 334, 691, 802, 828, 946
Hpy188III TCNNGA 3 cut(s) 179, 1169, 1190
Hpy8I GTNNAC 5 cut(s) 230, 259, 760, 892, 1071
Hpy99I CGWCG 3 cut(s) 10, 34, 224
HpyAV CCTTC 4 cut(s) 51, 166, 445, 751
HpyCH4III ACNGT 5 cut(s) 73, 145, 375, 640, 778
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 7 cut(s) 314, 466, 841, 1006, 1049, 1089, 1202
HpyF10VI GCNNNNNNNGC 5 cut(s) 246, 290, 1055, 1252, 1277
HpyF3I CTNAG 8 cut(s) 19, 55, 76, 111, 126, 236, 945, 1121
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 2 cut(s) 5, 29
Hsp92II CATG 6 cut(s) 472, 845, 972, 1010, 1151, 1193
HspAI GCGC 2 cut(s) 291, 1044
Kzo9I GATC 1 cut(s) 844
LmnI GCTCC 4 cut(s) 568, 728, 919, 1055
Lsp1109I GCAGC 3 cut(s) 493, 671, 1033
LweI GCATC 1 cut(s) 379
MaeI CTAG 2 cut(s) 345, 543
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 3 cut(s) 139, 614, 871
MalI GATC 1 cut(s) 846
MboI GATC 1 cut(s) 844
MboII GAAGA 7 cut(s) 36, 167, 298, 740, 759, 1044, 1236
MhlI GDGCHC 3 cut(s) 90, 1060, 1189
MluCI AATT 9 cut(s) 182, 336, 410, 461, 630, 932, 1173, 1240, 1261
MlyI GAGTC 2 cut(s) 47, 587
MmeI TCCRAC 5 cut(s) 437, 686, 697, 760, 968
MroXI GAANNNNTTC 2 cut(s) 807, 1014
MseI TTAA 3 cut(s) 296, 573, 1176
MslI CAYNNNNRTG 2 cut(s) 846, 1152
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 654
Mva1269I GAATGC 2 cut(s) 1136, 1271
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 2 cut(s) 135, 291
MwoI GCNNNNNNNGC 5 cut(s) 246, 290, 1055, 1252, 1277
NdeII GATC 1 cut(s) 844
NlaIII CATG 6 cut(s) 472, 845, 972, 1010, 1151, 1193
NlaIV GGNNCC 4 cut(s) 205, 211, 417, 1024
NmeAIII GCCGAG 2 cut(s) 298, 531
NmuCI GTSAC 3 cut(s) 139, 614, 871
NspI RCATGY 2 cut(s) 472, 972
PagI TCATGA 1 cut(s) 1189
PciI ACATGT 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 2 cut(s) 1136, 1271
PdmI GAANNNNTTC 2 cut(s) 807, 1014
PfeI GAWTC 2 cut(s) 263, 1076
PkrI GCNGC 6 cut(s) 134, 154, 290, 483, 686, 1048
PleI GAGTC 2 cut(s) 47, 586
PpsI GAGTC 2 cut(s) 47, 586
PscI ACATGT 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 652
PspEI GGTNACC 1 cut(s) 871
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 4 cut(s) 205, 211, 417, 1024
PspPI GGNCC 2 cut(s) 130, 415
RsaI GTAC 1 cut(s) 1231
RsaNI GTAC 1 cut(s) 1230
RseI CAYNNNNRTG 2 cut(s) 846, 1152
SaqAI TTAA 3 cut(s) 296, 573, 1176
SatI GCNGC 6 cut(s) 133, 153, 289, 482, 685, 1047
Sau3AI GATC 1 cut(s) 844
Sau96I GGNCC 2 cut(s) 130, 415
SchI GAGTC 2 cut(s) 47, 587
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 3 cut(s) 90, 1060, 1189
SfaNI GCATC 1 cut(s) 379
SfcI CTRYAG 1 cut(s) 48
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 2 cut(s) 846, 1152
Sse9I AATT 9 cut(s) 182, 336, 410, 461, 630, 932, 1173, 1240, 1261
SsiI CCGC 6 cut(s) 133, 153, 289, 546, 951, 972
SspMI CTAG 2 cut(s) 345, 543
StyD4I CCNGG 1 cut(s) 652
TaaI ACNGT 5 cut(s) 73, 145, 375, 640, 778
TaiI ACGT 1 cut(s) 8
TaqI TCGA 1 cut(s) 1168
TasI AATT 9 cut(s) 182, 336, 410, 461, 630, 932, 1173, 1240, 1261
TauI GCSGC 3 cut(s) 135, 155, 291
TfiI GAWTC 2 cut(s) 263, 1076
Tru1I TTAA 3 cut(s) 296, 573, 1176
Tru9I TTAA 3 cut(s) 296, 573, 1176
TseFI GTSAC 3 cut(s) 139, 614, 871
TseI GCWGC 3 cut(s) 481, 684, 1046
Tsp45I GTSAC 3 cut(s) 139, 614, 871
TspDTI ATGAA 6 cut(s) 391, 501, 546, 1023, 1143, 1206
TspGWI ACGGA 1 cut(s) 1056
VpaK11BI GGWCC 1 cut(s) 415
XagI CCTNNNNNAGG 2 cut(s) 114, 546
XapI RAATTY 3 cut(s) 336, 932, 1261
XceI RCATGY 2 cut(s) 472, 972
XmiI GTMKAC 2 cut(s) 759, 1070
XmnI GAANNNNTTC 2 cut(s) 807, 1014
XspI CTAG 2 cut(s) 345, 543
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.